publications by David Needham.

Papers Published

1. Hochmuth, R.M. and Ting-Beall, H.P. and Beaty, B.B. and Needham, D. and Tran-Son-Tay, R., Viscosity of passive human neutrophils undergoing small deformations, Biophys. J. (USA), vol. 64 no. 5 (1993), pp. 1596 - 601 .
   (last updated on 2007/04/06)

   Abstract:
   At issue is the type of constitutive equation that can be used to describe all possible types of deformation of the neutrophil. A neutrophil undergoing small deformations is studied by aspirating it into a glass pipet with a diameter that is only slightly smaller than the diameter of the spherically shaped cell. After being held in the pipet for at least seven seconds, the cell is rapidly expelled and allowed to recover its undeformed, spherical shape. The recovery takes ~15 s. An analysis of the recovery process that treats the cell as a simple Newtonian liquid drop with a constant cortical (surface) tension gives a value of 3.3×10^-5 cm/s for the ratio of the cortical tension to cytoplasmic viscosity. This value is about twice as large as a previously published value obtained with the same model from studies of large deformations of neutrophils. This discrepancy indicates that the cytoplasmic viscosity decreases as the amount of deformation decreases

   Keywords:
   cellular biophysics;haemorheology;viscosity;