- Needham, David and Noppl, Doris, Adsorption and adhesion at vesicle surfaces: the influence of grafted polymer on avidin-biotin binding and tests of receptor-mediated adhesion,
American Society of Mechanical Engineers, Bioengineering Division (Publication) BED, vol. 29
pp. 331 - 332 .
(last updated on 2007/04/06)
Micropipette techniques coupled to fluorescence video microscopy are used to carry out two kinds of experiments on adsorption and adhesion at vesicle surfaces. The model system of biotinylated lipid vesicles with avidin is also used. The binding of avidin to a single biotinylated vesicle is measured in the absence and presence of surface grafted polyethylene glycol. Likewise, adhesion energies are measured for two vesicles that are brought into adherent contact where adhesion results only from the formation of biotin-avidin-biotin cross-bridges.
Adhesion;Proteins;Graft copolymers;Thermodynamics;Diffusion;Polyethylene glycols;Lipids;Fluorescence;Microscopic examination;Molecular weight;