Papers Published

1. Ho, Y. P. and Chen, H. H. and Leong, K. W. and Wang, T. H., The convergence of quantum-dot-mediated fluorescence resonance energy transfer and microfluidics for monitoring DNA polyplex self-assembly in real time, Nanotechnology, vol. 20 no. 9 (2009), pp. - .
   (last updated on 2010/06/11)

   Abstract:
   We present a novel convergence of quantum-dot-mediated fluorescence resonance energy transfer (QD-FRET) and microfluidics, through which molecular interactions were precisely controlled and monitored using highly sensitive quantum-dot-mediated FRET. We demonstrate its potential in studying the kinetics of self-assembly of DNA polyplexes under laminar flow in real time with millisecond resolution. The integration of nanophotonics and microfluidics offers a powerful tool for elucidating the formation of polyelectrolyte polyplexes, which is expected to provide better control and synthesis of uniform and customizable polyplexes for future nucleic acid-based therapeutics.

   Keywords:
   gene-therapy progress delivery chitosan nanoparticles complexes efficiency condensation channels flow deacetylation