- Mao, H. Q. and Leong, K. W., Design of polyphosphoester-DNA nanoparticles for non-viral gene delivery,
Adv Genet, vol. 53
pp. 275-306 .
(last updated on 2010/06/11)
Development of safe and effective non-viral gene carriers is still critical to the ultimate success of gene therapy. This review highlights our attempt to design the gene carriers in a systematic manner. We have synthesized a series of polymers with a phosphoester backbone containing different charge groups in the sidechain connected to the backbone through a phosphate (P-O) or a phosphoramide (P-N) bond. These gene carriers have different charge groups, sidechain lengths, and branching structures, but they are structurally related to allow a systematic investigation of the structure-property relationship, including DNA binding capacity, cytotoxicity, DNA protection, biodegradability, DNA release kinetics, and transfection efficiency.
Biological Transport/physiology DNA/genetics/*metabolism Gene Therapy/*methods *Gene Transfer Techniques Genetic Vectors/genetics/*metabolism Imidazoles/chemistry/metabolism *Nanostructures Phosphoric Acid Esters/chemistry/*metabolism Plasmids/genetics/physiology Polymers/chemistry/*metabolism