- Chai, C. and Feng, Q. and Jiang, X.S. and Leong, K.W. and Mao, H.Q., Expansion of CD34+ cells on polymeric scaffolds surface-immobilized with hematopoietic growth factors,
Transactions - 7th World Biomaterials Congress
pp. 897 - .
(last updated on 2007/04/13)
The expansion of CD34+ hematopoietic stem cells (HSC) on two-dimensional (2D) and three-dimensional (3D) polymeric scaffolds, surface-immobilized with hematopoietic growth factors (HGF), was investigated. The polymeric scaffolds were prepared by functionalizing polyethylene terephthalate (PET) film disks and non-woven disks with carboxyl (COOH) groups by oxidation with potassium permanganate. Stem cell factor (SCF) and IL-6 HGFs were covalently immobilized individually or concomitantly into PET-COOH via amide bond formation. It was observed that immobilized IL-6 on 2D PET surface induced a dramatic expansion of CD34+ cells, contributed largely by expansion of progenitor cells with multi-lineage potential.
Biomaterials;Polyethylene terephthalates;Growth kinetics;Cell immobilization;Cell membranes;Solubility;Bone;Cytology;Oxidation;