- Chua, K.N. and Lim, W.S. and Lu, H.F. and Chai, C. and Zhang, P.C. and Wen, J. and Ramakrishna, S. and Leong, K.W. and Mao, H.Q., Immobilized hepatocyte spheroids on galactose ligand-conjugated nanofiber scaffold,
Transactions - 7th World Biomaterials Congress
pp. 1753 - .
(last updated on 2007/04/13)
The effect of nanofiber scaffold on hepatocyte attachment, migration, spheroid formation and maintenance if the differentiated functions was analyzed. A hepatocyte specific nanofiber scaffold was synthesized by conjugating 1-O-(6'-aminohexyl)-D-galactopyranoside (AHG) galactose ligands to the poly(acrylic acid) grafted polycaprolactone (PCL)-co- ethylethylene phosphate (EEP) nanofiber mesh. It was found that hepatocytes cultured on galactosylated PCL-co-EEP nanofiber scaffolds and thin film substrates exhibited similarly high hepatocyte attachment (~80-90%). The results show that the nanofiber scaffolds exhibits unique properties of promoting hepatocyte aggregates within the spaces and around the fiber.
Enzyme inhibition;Fibers;Nanostructured materials;Monolayers;Scaffolds;Polyethylene terephthalates;Thin films;Substrates;Synthesis (chemical);Polymerization;Morphology;