Papers Published

  1. Chua, K.N. and Lim, W.S. and Lu, H.F. and Chai, C. and Zhang, P.C. and Wen, J. and Ramakrishna, S. and Leong, K.W. and Mao, H.Q., Immobilized hepatocyte spheroids on galactose ligand-conjugated nanofiber scaffold, Transactions - 7th World Biomaterials Congress (2004), pp. 1753 - .
    (last updated on 2007/04/13)

    The effect of nanofiber scaffold on hepatocyte attachment, migration, spheroid formation and maintenance if the differentiated functions was analyzed. A hepatocyte specific nanofiber scaffold was synthesized by conjugating 1-O-(6'-aminohexyl)-D-galactopyranoside (AHG) galactose ligands to the poly(acrylic acid) grafted polycaprolactone (PCL)-co- ethylethylene phosphate (EEP) nanofiber mesh. It was found that hepatocytes cultured on galactosylated PCL-co-EEP nanofiber scaffolds and thin film substrates exhibited similarly high hepatocyte attachment (~80-90%). The results show that the nanofiber scaffolds exhibits unique properties of promoting hepatocyte aggregates within the spaces and around the fiber.

    Enzyme inhibition;Fibers;Nanostructured materials;Monolayers;Scaffolds;Polyethylene terephthalates;Thin films;Substrates;Synthesis (chemical);Polymerization;Morphology;