- Lim, W.S. and Chua, K.N. and Wang, X.L. and Zhang, P.C. and Lu, H.F. and Leong, K.W. and Mao, H.Q., Transfection of rat primary hepatocytes using a novel polyphosphoramidate gene carrier,
Transactions - 7th World Biomaterials Congress
pp. 1813 - .
(last updated on 2007/04/13)
The transfectability of the hepatocyte spheroids in comparison with hepatocyte monolayer was investigated using a novel non-viral polycationic gene carrier, polyphosphoramidate containing diethylamine side chains (PPA-DEA). The optimal window for transfection after cell seeding and culture was also determined. Using ELISA and HPLC techniques, hepatocyte-specific functions such as albumin secretion and P450 activity were measured. PPA-DEA was found to be a viable non-viral gene carrier since it produces reasonable transgene expression and has no adverse effects on the hapatocyte-specific functions.
Cells;Proteins;Monolayers;Collagen;Tissue;Polystyrenes;Synthesis (chemical);DNA;High performance liquid chromatography;Polyethylene terephthalates;