- Lu, Hong-Fang and Lim, Wei Seng and Wang, Jun and Tang, Zhi-Qun and Zhang, Peng-Chi and Leong, Kam W. and Chia, Ser Mien and Yu, Hanry and Mao, Hai-Quan, Galactosylated PVDF membrane promotes hepatocyte attachment and functional maintenance,
Biomaterials, vol. 24 no. 27
pp. 4893 - 4903 [S0142-9612(03)00404-6] .
(last updated on 2007/04/13)
One of the major challenges in BLAD design is to develop functional substrates suitable for hepatocyte attachment and functional maintenance. In the present study, we designed a poly(vinylidene difluoride) (PVDF) surface coated with galactose-tethered Pluronic polymer. The galactose-derived Pluronic F68 (F68-Gal) was adsorbed on PVDF membrane through hydrophobic-hydrophobic interaction between PVDF and the polypropylene oxide segment in Pluronic. The galactose density on the modified PVDF surface increased with the concentration of the F68-Gal solution, reaching 15.4nmol galactosyl groups per cm2 when a 1mg/ml of F68-Gal solution was used. The adsorbed F68-Gal remained relatively stable in culture medium. Rat hepatocytes attachment efficiency on F68-Gal modified PVDF membrane was similar to that on collagen-coated surface. The attached hepatocytes on PVDF/F68-Gal membrane self-assembled into multi-cellular spheroids after 1 day of culture. These attached hepatocytes in spheroids exhibited higher cell functions such as albumin synthesis and P450 1A1 detoxification function compared to unmodified PVDF membrane and collagen-coated surface. These results suggest the potential of this galactose-immobilized PVDF membrane as a suitable substrate for hepatocyte culture. © 2003 Elsevier Ltd. All rights reserved.
Adsorption;Hydrophobicity;Density (specific gravity);Solutions;Collagen;Substrates;