- Brown, Kimberly E. and Shao, Wen and Bathon, Joan and Leong, Kam W., Controlled drug delivery to the joints by enzymatically degradable microspheres,
Materials Research Society Symposium Proceedings, vol. 331
pp. 73 - 78 .
(last updated on 2007/04/13)
An intra-articular polymeric controlled release system was developed that is tailored to, and responsive to, the intensity of joint inflammation. Microspheres composed of the naturally occurring polyelectrolytes, gelatin and chondroitin sulfate, were synthesized by complex coacervation and the kinetics of release of encapsulated 14C-catalase, was evaluated in vitro in the presence of inflammatory and non-inflammatory human joint fluids. The relative activity of gelatinase, a metalloprotease enzyme, was quantified in each of the joints fluids. Rate of degradation of microspheres, and consequent release of 14C-catalase, was found to parallel the relative gelatinase activities in the joint fluids. Furthermore, various methods of crosslinking were found to affect the kinetics of microsphere degradation in the fluids. A catalase loading level of up to 28% was achieved, and the encapsulated catalase was found to retain up to 58% of its biological activity.
Joints (anatomy);Biodegradation;Biomedical engineering;Biopolymers;Polyelectrolytes;Reaction kinetics;Crosslinking;Enzymes;