- Yin, Chao and Ying, Lei and Zhang, Peng-Chi and Zhuo, Ren-Xi and Kang, En-Tang and Leong, Kam W. and Mao, Hai-Quan, High density of immobilized galactose ligand enhances hepatocyte attachment and function,
Journal of Biomedical Materials Research - Part A, vol. 67 no. 4
pp. 1093 - 1104 [jbm.a.10033] .
(last updated on 2007/04/13)
Galactosylated surface is an attractive substrate for hepatocyte culture because of the specific interaction between the galactose ligand and the asialoglycoprotein receptor on hepatocytes. In this study, we described a scheme to achieve high density of immobilized galactose ligands on polyethylene terephthalate (PET) surface by first surface-grafting polyacrylic acid on plasma-pretreated PET film under UV irradiation, followed by conjugation of a galactose derivative (1-O-(6 prime -aminohexyl)-D-galactopyranoside) to the grafted polyacrylic acid chains. A high galactose density of 513 nmol/cm 2 on the PET surface was used in this study to investigate the behavior of cultured hepatocyte. This engineered substrate showed high affinity to fluorescein isothiocyanate-lectin binding. Primary rat hepatocytes, when seeded at a density of 2 x 105 cells/cm2, attached to the galactosylated PET substrate at a similar efficiency compared with collagen-coated substrate. The hepatocytes spontaneously formed aggregates 1 day after cell seeding and showed better maintenance of albumin secretion and urea synthesis functions than those cultured on collagen-coated surface. © 2003 Wiley Periodicals, Inc.
Collagen;Polyethylene terephthalates;Grafting (chemical);Ultraviolet radiation;Irradiation;Substrates;Synthesis (chemical);