- Pan, Wenxiao and Petersen, Erik and Cai, Ning and Ma, Gang and Lee, Jian Run and Feng, Zhiqin and Liao, Kin and Leong, Kam W., Viscoelastic properties of human mesenchymal stem cells,
Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings, vol. 7 S
pp. 4854 - 4857 .
(last updated on 2007/04/13)
In this study, we investigated the viscoelasticity of individual bone marrow-derived adult human Mesenchymal Stem Cells (hMSCs), and the role of specific cytoskeletal component-F-actin microfilaments on the mechanical properties of individual hMSCs. The mechanical properties of hMSCs were determined using the micropipette aspiration technique coupled with a viscoelastic solid model of the cell. For the hMSCs under control conditions the instantaneous Young's modulus E0 was found to be 886±289(Pa), the equilibrium Young's modulus E infinity 372±125(Pa), and the apparent viscosity μ, 2714±1626(Pa·S). After exposed to 2μM of chemical agent-cytochalasin D that disrupt the F-actin microfilaments, the Young's moduli of hMSCs decreased by up to 72% and the apparent viscosity increased by 167%. These findings suggest that microfilaments are crucial in providing the viscoelastic properties of the hMSCs, and changes in the structure and properties of them may influence the mechanical properties of hMSCs significantly. © 2005 IEEE.
Antibiotics;Elastic moduli;Mechanical properties;Muscle;Proteins;Viscoelasticity;