- Brown, K.E. and Bathon, J. and Huang, C.H. and Dalal, R. and Leong, K.W., Cationic gelatin as a gene carrier,
Materials Research Society Symposium - Proceedings, vol. 394
pp. 61 - 66 .
(last updated on 2007/04/13)
Cationic gelatin (CG) has been evaluated as a non-viral vector for cell transfection. CG is synthesized by modifying gelatin with hexanediamine. Its complexation with psv-8-gal plasmid caused an electrophoretic mobility shift of the plasmid. CG/DNA complexes are optimized in terms of transfection efficiency in CHODUK XB1 and COS 7 cell lines. Maximal gene expression for both cell types occurred in serum free medium with chloroquine at cationic gelatin/DNA ratios of about two and seven. Compared to DEAF dextran, polysine and Lipofectamine, CG is the most efficient in transfecting COS 7 cells. In a dye reduction cytotoxicity assay, CG caused <5% of cells to become nonviable at a concentration of 100 μg/m1, while other transfection reagents tested caused 25-100% of cell death.
Genes;Cells;Synthesis (chemical);Complexation;DNA;Drug products;Toxicity;Bioassay;