- Feng, Q. and Jiang, X.S. and Chai, C. and Lim, W.S. and Leong, K.W. and Mao, H.Q., Ex vivo expansion of hematopoietic primitive cells on ECM molecule-immobilized scaffold,
Transactions - 7th World Biomaterials Congress
pp. 898 - .
(last updated on 2007/04/13)
The ex vivo expansion of hematopoietic stem sells (HSC) on extracellular matrix (ECM) molecule-immobilized scaffold was investigated. The scaffold used was a fibronectin and collagen conjugated polyethylene terephthalate (PET) scaffold. Bone marrow CD34+ cells were cultured in the modified PET scaffolds in a 96-well plate using serum free StemSpam medium supplemented with cytokines. It was observed that the scaffolds surface-conjugated with ECM molecules served as an effective substrate for the expansion of hematopoietic primitive cells. Fibronectin conjugated scaffold was found to exhibit the highest extent of CD34+ cell expansion.
Cell immobilization;Bone;Collagen;Polyethylene terephthalates;Transplantation (surgical);Medical problems;Disease control;Cell membranes;Solubility;Suspensions (fluids);pH effects;Crosslinking;