- Oberhauser, A. F. and Marszalek, P. E. and Carrion-Vazquez, M. and Fernandez, J. M., Single protein misfolding events captured by atomic force microscopy,
Nature Structural Biology, vol. 6 no. 11
pp. 1025-1028 .
(last updated on 2007/11/30)
Using single protein atomic force microscopy (AFM) techniques we demonstrate that after repeated mechanical extension/relaxation cycles, tandem modular proteins tan misfold into a structure formed by two neighboring modules. The misfolding is fully reversible and alters the mechanical topology of the modules while it is about as stable as the original fold. Our results show that modular proteins can assume a novel misfolded state and demonstrate that AFM is able to capture, in real time, rare misfolding events at the level of a single protein.