Papers Published

  1. Needham, D. and Hochmuth, R.M., Electro-mechanical permeabilization of lipid vesicles. Role of membrane tension and compressibility, Biophys. J. (USA), vol. 55 no. 5 (1989), pp. 1001 - 9 .
    (last updated on 2007/04/10)

    A simple micropipet technique was used to determine the critical electric field strength for membrane breakdown as a function of the applied membrane tension for three different reconstituted membranes: stearoyloleoylphosphatidylcholine (SOPC), red blood cell (RBC) lipid extract, and SOPC cholesterol (CHOL), 1:1. For these membranes the elastic area expansivity modulus increases from ~200 to 600 dyn/cm, and the tension at lysis increases from 5.7 to 13.2 dyn/cm, i.e., the membranes become more cohesive with increasing cholesterol content. The critical membrane voltage, Vc, required for breakdown was also found to increase with increasing cholesterol from 1.1 to 1.8 V at zero membrane tension

    bioelectric phenomena;biomechanics;biomembrane transport;lipid bilayers;