- Albarran, Brian and Ting-Beall, H. Ping and Zhelev, Doncho V. and Hochmuth, Robert M., Effect of membrane area expansion on the cortical tension of human neutrophils,
Annals of Biomedical Engineering, vol. 28 no. SUPPL 1
pp. -75 - .
(last updated on 2007/04/10)
Micropipette techniques were used to measure the effect of membrane area expansion on cortical tension. Two sets of experiments were made: one set had neutrophils suspended in hypnotic solutions and the other had neutrophils aspirated into one pipette. Thus, the postulated, persistent cortical tension doesn't appear to be a unique and constant parameter for neutrophils as the membrane area is dilated by either osmotic or mechanical force. Furthermore, cells osmotically contracted to varying degrees did not behave as a liquid drop but rather as a viscoelastic solid and exhibited values of approx. 3.5-8 pN/μm2 for the Young's modulos, E, in an osmolaritydependent fushion.
Blood;Cytology;Cell membranes;Biomedical engineering;Elastic moduli;