- Zhelev, Doncho V. and Alteraifi, Abdullatif M. and Hochmuth, R.M., Network formation and contraction in activated human neutrophils,
American Society of Mechanical Engineers, Bioengineering Division (Publication) BED, vol. 29
pp. 395 - 396 .
(last updated on 2007/04/10)
Four processes involve in the formation of new cytoskeletal network and its disassembly have been discussed, which include crawling inside a pipet, phagocytosis, polymerization inside tethers, and pseudogrowth. Network formation and disassembly are thus coupled, and determine the network dynamics. This dynamics, however, cannot be characterized directly; the experimentally measured parameters that is closely related to it is the advancement of network front or network 'boundary'. Accordingly, the front advancement along a tether is studied for different temperatures for a medium containing Ca2+ and for Ca2+-free medium. The slope of the Arrhenius plot of this dependence is hence related to the activation energy of network formation.
Shrinkage;Cytology;Surface tension;Force measurement;Stresses;Stiffness;Stress relaxation;Growth kinetics;Polymerization;Mass transfer;Activation energy;Diffusion;