- Mackie, L.H. and Frank, R.S. and Hochmuth, R.M., Erythrocyte density separation on discontinuous `Percoll' gradients,
Biorheology (UK), vol. 24 no. 2
pp. 227 - 30 .
(last updated on 2007/04/10)
A relatively easy and straightforward method has been developed to density separate erythrocytes using discontinuous `Percoll' gradients. Percoll (Pharmacia; Uppsala, Sweden, Sigma; St. Louis, MO) is a suspension of polyvinylpyrrolidone coated 21 nm diameter silica particles with a density of 1.13 g/ml and an osmolality of 20 mosmol/kg. Percoll can be used to make continuous (13-15) or discontinuous density gradients (16,17); however, the authors obtained better erythrocyte separation and yield using discontinuous gradients. With discontinuous gradients, cells are separated into discrete bands with densities between the densities of the adjacent layers. With Percoll as the base, solutions can be prepared that range in density from 1.090 g/ml to 1.148 g/ml. This range allows separation over the full span of erythrocyte densities
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