Publications of Daniele Armaleo :chronological combined listing:
%% Papers Published
@article{fds152793,
Author = {D. Armaleo and Yi Zhang and Sonia Cheung},
Title = {Light might regulate divergently depside and depsidone
accumulation in the lichen Parmotrema hypotropum by
affecting thallus temperature and water potential},
Journal = {Mycologia},
Volume = {100},
Number = {4},
Pages = {565-576},
Year = {2008},
ISSN = {Online: 1557-2536 Print: 0027-5514},
url = {http://www.mycologia.org/content/vol100/issue4/#LICHENS},
Abstract = {Depsides and depsidones are the most common secondary
products uniquely produced in lichens by the fungal
symbiont, and they accumulate on the outer surface of its
hyphae. Their biological roles are subject to debate.
Quantitatively the compounds typical of a given lichen can
vary dramatically from thallus to thallus. Several studies
have addressed whether this variability is correlated with
the light reaching different thalli, but the conclusions are
contradictory. We addressed the question with the lichen
Parmotrema hypotropum growing on unshaded, vertical tree
trunks, a controlled natural environment where the light
absorbed by each thallus over its lifetime is the only major
position-dependent variable. The exact north-east-south-west
orientation of each thallus was used to calculate its yearly
light exposure based on astronomical and meteorological
considerations. The calculated irradiation around the trunk,
distributed over a continuous 40-fold intensity range, then
was compared with the amount of compound per unit thallus
weight, determined by quantitative thin layer
chromatography. P. hypotropum accumulates the depside
atranorin in the cortex and the depsidone norstictic acid in
the medulla and around the algae. A direct correlation was
observed between the yearly amount of light reaching the
lichen and the amount of atranorin. In contrast, the amount
of norstictic acid decreased with increasing light. Although
we did not measure thallus temperature and water potential,
a unifying interpretation of these and other published data
is that depside/depsidone accumulation in lichens is
mediated by localized changes in temperature and water
potential produced by light absorption within each thallus.
This suggests water relations-based functions for depsides
and depsidones.},
Key = {fds152793}
}
@article{fds33077,
Author = {D. Armaleo and V. Miao},
Title = {Symbiosis and DNA methylation in the Cladonia lichen
fungus},
Volume = {26},
Pages = {143-163},
Year = {1999},
Abstract = {In eukaryotes, the modification of DNA by addition or
removal of specific methyl groups is thought to affect gene
activity and differentiation. We began to investigate the
relationship between DNA methylation and differentiation in
lichens, organisms in which the symbiosis between a fungus
and a unicellular alga or cyanobacterium generates
remarkable morphological and biochemical complexity.
Restriction analysis of DNA from the lichen Cladonia grayi
indicated that overall DNA methylation is low in the fungus
cultured in absence of the alga and high in the natural
lichen. Within the lichen, however, fungal DNA methylation
is not uniform: it is high in the body of the goblet-shaped
thalli (podetia) and in the vegetative propagules (soredia),
somatic tissues in which the fungus is associated with the
alga; it is low in the fungal ascocarps (apothecia) which
develop without algae on the upper rim of the podetia and
produce meiotic spores. Methylation remains low in the
mycelia derived from spores in axenic culture. These results
suggest a correlation between symbiosis and methylation of
fungal DNA in Cladonia. DNA methylation was also observed in
two other lichens tested, a Parmelia and an Usnea. To relate
the overall genomic changes found in Cladonia to the
behavior of individual genes, we evaluated through Southern
blotting the methylation of four fungal genes presumably
involved in the production of lichen secondary compounds:
three appear more methylated in the lichen than in the
isolated fungus, while one shows the opposite
behavior.},
Key = {fds33077}
}
@article{fds33076,
Author = {D. Armaleo and Philippe Clerc},
Title = {A rapid and inexpensive method for the purification of DNA
from lichens and their symbionts},
Journal = {The Lichenologist},
Volume = {27},
Pages = {207-213},
Year = {1995},
Key = {fds33076}
}
@article{fds33075,
Author = {D. Armaleo},
Title = {Factors affecting depside and depsidone biosynthesis in a
cultured lichen fungus},
Journal = {Cryptogamic Botany},
Volume = {5},
Pages = {14-21},
Year = {1992},
Key = {fds33075}
}
@article{fds33074,
Author = {C.F. Culberson and D. Armaleo},
Title = {Induction of a complete secondary-product pathway in a
cultured lichen fungus},
Journal = {Experimental Mycology},
Volume = {16},
Pages = {53-63},
Year = {1992},
Key = {fds33074}
}
@article{fds33073,
Author = {D. Armaleo},
Title = {Experimental microbiology of lichens: mycobiont
fragmentation, a novel growth chamber, and the origins of
thallus differentiation},
Journal = {Symbiosis},
Volume = {11},
Pages = {163-178},
Year = {1991},
Key = {fds33073}
}
@article{fds33072,
Author = {D. Armaleo and P. Clerc},
Title = {Lichen chimeras: DNA analysis suggests that one fungus forms
two morphotypes},
Journal = {Experimental Mycology},
Volume = {15},
Pages = {1-10},
Year = {1991},
Key = {fds33072}
}
@article{fds33071,
Author = {D. Armaleo and G. Ye and T.M. Klein and K. Shark and S. Johnston and J.C. Sanford},
Title = {Biolistic nuclear transformation of Saccharomyces cerevisiae
and other fungi},
Journal = {Current Genetics},
Volume = {17},
Pages = {97-103},
Year = {1990},
Key = {fds33071}
}
@article{fds33070,
Author = {D. Armaleo},
Title = {Structure and evolution of prokaryotic and eukaryotic RNA
polymerases: a model},
Journal = {Journal of Theoretical Biology},
Volume = {127},
Pages = {301-314},
Year = {1987},
Key = {fds33070}
}
@article{fds33068,
Author = {D. Armaleo and S.R. Gross},
Title = {Structural studies on Neurospora RNA polymerases and
associated proteins},
Journal = {Journal of Biological Chemistry},
Volume = {260},
Pages = {16174-16180},
Year = {1985},
Key = {fds33068}
}
@article{fds33069,
Author = {D. Armaleo and S.R. Gross},
Title = {Effect of alpha-isopropylmalate on the synthesis of RNA and
protein in Neurospora},
Journal = {Molecular and General Genetics},
Volume = {200},
Pages = {346-349},
Year = {1985},
Key = {fds33069}
}
@article{fds33065,
Author = {D. Armaleo and S.R. Gross},
Title = {Purification of the three nuclear RNA polymerases from
Neurospora crassa},
Journal = {Journal of Biological Chemistry},
Volume = {260},
Pages = {16169-16173},
Year = {1985},
Key = {fds33065}
}
%% Papers Submitted
@article{fds152795,
Author = {D. Armaleo and Susan May},
Title = {Sizing the fungal and algal genomes of the lichen Cladonia
grayi through quantitative PCR},
Journal = {Symbiosis},
Year = {2009},
Abstract = {Using a method based on quantitative PCR, we determined that
the nuclear genome sizes for the mycobiont and photobiont of
the lichen Cladonia grayi are 30 Mb and 100 Mb respectively.
This is the first genome size determination for lichens, and
suggests that between 15,000 and 30,000 genes are needed for
this symbiosis. While the genome size for the mycobiont is
average among fungi, that for the photobiont is at the
smaller end of the algal range. Genomes in this size range
can be sequenced at relatively low cost with current
pyrosequencing-based methods. The genome sizing method
requires very small amounts of DNA and should be applicable
to any lichen whose symbionts can be reliably isolated from
one another. Since the symbionts used in this project were
isolated from soredia, the lichen’s vegetative propagules,
we also describe a method for the establishment of axenic
symbiont cultures from large numbers of soredia.},
Key = {fds152795}
}
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