Publications of G. Allan Johnson :chronological by type listing:
%%
@article{fds132726,
Author = {G.A. Johnson},
Title = {1. C.F. Beaulieu, X. Zhou, G.P. Cofer, G.A. Johnson.
Diffusion-Weighted MR Microscopy with Fast Spin-Echo.
Magnetic Resonance in Medicine 30, 201-206
(1993).},
Year = {1994},
Key = {fds132726}
}
@article{fds132716,
Author = {G.A. Johnson},
Title = {1. Cho ZH, Ro YM. Multipoint K-Space Mapping Technique.
Reviewed for Magn Reson. in Med.},
Year = {1994},
Key = {fds132716}
}
@booklet{Mcadams85a,
Author = {McAdams, HP and Johnson, GA and Suddarth, SA and Ravin,
CE},
Title = {105 Optimization of Adaptive Filtration for Digital Chest
Imaging},
Journal = {Investigative Radiology},
Volume = {20},
Number = {6},
Pages = {S27-S27},
Publisher = {Ovid Technologies (Wolters Kluwer Health)},
Year = {1985},
Month = {September},
ISSN = {0020-9996},
url = {http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:A1985ARG0500122&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=47d3190e77e5a3a53558812f597b0b92},
Doi = {10.1097/00004424-198509000-00133},
Key = {Mcadams85a}
}
@booklet{Godwin84,
Author = {Godwin, JD and Pelc, NJ and Johnson, GA and Fram, EK and C,
R},
Title = {106. PROGRESS IN ECG-GATED CT},
Journal = {Investigative Radiology},
Volume = {19},
Number = {5},
Pages = {S27-S27},
Publisher = {Ovid Technologies (Wolters Kluwer Health)},
Year = {1984},
Month = {September},
ISSN = {0020-9996},
url = {http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:A1984TL42800126&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=47d3190e77e5a3a53558812f597b0b92},
Doi = {10.1097/00004424-198409000-00131},
Key = {Godwin84}
}
@booklet{Blinder85,
Author = {Blinder, RA and Herfkens, RJ and Johnson, GA and Coleman,
RE},
Title = {121 SURFACE COIL MOTION ARTIFACTS IN MR IMAGING},
Journal = {Investigative Radiology},
Volume = {20},
Number = {6},
Pages = {S31-S31},
Publisher = {Ovid Technologies (Wolters Kluwer Health)},
Year = {1985},
Month = {September},
ISSN = {0020-9996},
url = {http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:A1985ARG0500138&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=47d3190e77e5a3a53558812f597b0b92},
Doi = {10.1097/00004424-198509000-00149},
Key = {Blinder85}
}
@booklet{Johnson84,
Author = {Johnson, GA and Herfkens, RJ and MacFall, JR},
Title = {129. IN VIVO FIELD DEPENDENCE OF TISSUE RELAXATION
TIMES},
Journal = {Investigative Radiology},
Volume = {19},
Number = {5},
Pages = {S33-S33},
Publisher = {Ovid Technologies (Wolters Kluwer Health)},
Year = {1984},
Month = {September},
ISSN = {0020-9996},
url = {http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:A1984TL42800149&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=47d3190e77e5a3a53558812f597b0b92},
Doi = {10.1097/00004424-198409000-00154},
Key = {Johnson84}
}
@booklet{Stude84,
Author = {Stude, R and Hedlund, L and Johnson, GA and Thompson,
WM},
Title = {159. A CT EVALUATION OF THREE HEPATOBILIARY CONTRAST
AGENTS},
Journal = {Investigative Radiology},
Volume = {19},
Number = {5},
Pages = {S40-S40},
Publisher = {Ovid Technologies (Wolters Kluwer Health)},
Year = {1984},
Month = {September},
ISSN = {0020-9996},
url = {http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:A1984TL42800179&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=47d3190e77e5a3a53558812f597b0b92},
Doi = {10.1097/00004424-198409000-00184},
Key = {Stude84}
}
@booklet{Herfkens84,
Author = {Herfkens, RJ and Johnson, GA and Glover, G},
Title = {166. IN VIVO CHEMICAL SHIFT IMAGING OF HYDROGEN},
Journal = {Investigative Radiology},
Volume = {19},
Number = {5},
Pages = {S42-S42},
Publisher = {Ovid Technologies (Wolters Kluwer Health)},
Year = {1984},
Month = {September},
ISSN = {0020-9996},
url = {http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:A1984TL42800185&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=47d3190e77e5a3a53558812f597b0b92},
Doi = {10.1097/00004424-198409000-00191},
Key = {Herfkens84}
}
@booklet{Hedlund87,
Author = {Hedlund, L and Cofer, G and Suddarth, S and Johnson,
GA},
Title = {177. SMALL ANIMAL ANESTHESIA AND MONITORING DURING EXTENDED
MR MICROSCOPY},
Journal = {Investigative Radiology},
Volume = {22},
Number = {9},
Pages = {S44-S44},
Publisher = {Ovid Technologies (Wolters Kluwer Health)},
Year = {1987},
Month = {September},
ISSN = {0020-9996},
url = {http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:A1987K178300189&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=47d3190e77e5a3a53558812f597b0b92},
Doi = {10.1097/00004424-198709000-00193},
Key = {Hedlund87}
}
@booklet{Sherrier84,
Author = {Sherrier, RH and Johnson, GA and Ravin, CE and Suddarth,
SA},
Title = {194. DIGITAL SYNTHESIS OF LUNG NODULES},
Journal = {Investigative Radiology},
Volume = {19},
Number = {5},
Pages = {S49-S49},
Publisher = {Ovid Technologies (Wolters Kluwer Health)},
Year = {1984},
Month = {September},
ISSN = {0020-9996},
url = {http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:A1984TL42800213&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=47d3190e77e5a3a53558812f597b0b92},
Doi = {10.1097/00004424-198409000-00219},
Key = {Sherrier84}
}
@article{fds174087,
Author = {GA Johnson and SJ Boukma and PA Platz},
Title = {2-mercaptobenzothiazole, an inhibitor of dopamine
beta-hydroxylase.},
Journal = {The Journal of pharmacy and pharmacology},
Volume = {22},
Number = {9},
Pages = {710-2},
Year = {1970},
Month = {September},
ISSN = {0022-3573},
Keywords = {Animals • Brain Chemistry • Dopamine • Heart
• Metaraminol • Mice • Mixed Function
Oxygenases • Motor Activity • Myocardium •
Norepinephrine • Rats • Thiazoles • analysis
• antagonists & inhibitors* • drug effects •
pharmacology • pharmacology*},
Language = {eng},
Key = {fds174087}
}
@article{fds132727,
Author = {G.A. Johnson},
Title = {2. H. Benveniste, G. Johnson, in "Microcirculatory Stasis in
the Brain" (M. Tomita, Mchedlishvili, W. Roseblum, W.D.
Heiss, Y. Fukuuchi, Ed.), p. 369-376, Excerpta Medica,
Amsterdam, 1993.},
Year = {1994},
Key = {fds132727}
}
@article{fds132717,
Author = {G.A. Johnson},
Title = {2. Hafner S, Kuhn W. NMR Imaging of Water Contentin Chips.
Reviewed for Magn. Reson. Imaging.},
Year = {1994},
Key = {fds132717}
}
@booklet{Johnson86a,
Author = {JOHNSON, GA and THOMPSON, MB and DRAYER, BP},
Title = {3-DIMENSIONAL MRI MICROSCOPY OF THE RAT-BRAIN},
Journal = {INVESTIGATIVE RADIOLOGY},
Volume = {21},
Number = {9},
Pages = {S25-S25},
Publisher = {LIPPINCOTT-RAVEN PUBL},
Year = {1986},
Month = {September},
ISSN = {0020-9996},
url = {http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:A1986E000900109&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=47d3190e77e5a3a53558812f597b0b92},
Key = {Johnson86a}
}
@booklet{Macfall95,
Author = {MACFALL, IS and JOHNSON, GA},
Title = {3-DIMENSIONAL ROOT-GROWTH, TURNOVER AND TRANSPORT AS SEEN
WITH MAGNETIC-RESONANCE MICROSCOPY},
Journal = {PLANT PHYSIOLOGY},
Volume = {108},
Number = {2},
Pages = {31-31},
Publisher = {AMER SOC PLANT PHYSIOLOGISTS},
Year = {1995},
Month = {June},
ISSN = {0032-0889},
url = {http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:A1995RE28900117&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=47d3190e77e5a3a53558812f597b0b92},
Key = {Macfall95}
}
@booklet{Lester99a,
Author = {Lester, DS and Lyon, RC and McGregor, GN and Engelhardt, RT and Schmued,
LC and Johnson, GA and Johannessen, JN},
Title = {3-Dimensional visualization of lesions in rat brain using
magnetic resonance imaging microscopy.},
Journal = {Neuroreport},
Volume = {10},
Number = {4},
Pages = {737-741},
Year = {1999},
Month = {March},
ISSN = {0959-4965},
url = {http://www.ncbi.nlm.nih.gov/pubmed/10208540},
Abstract = {High-resolution (< 50 microm) magnetic resonance imaging
microscopy (MRM) has been used to identify brain regions and
localization of excitotoxin-induced lesions in fixed rat
brains, subsequently confirmed using standard histology. The
anatomical extent of lesions identified by MRM was identical
to that seen in histological sections and various
histopathological changes could be visualized. In contrast
to the time involved in preparing and examining histological
sections, lesions in intact brains could be rapidly
identified and visualized in three dimensions by examining
digitally generated sections in any plane. This study shows
that MRM has tremendous potential as a prescreening tool for
neurotoxicity and neuropathology. These observations suggest
that MRM has the potential to affect pathology much as
conventional MRI has influenced clinical
imaging.},
Doi = {10.1097/00001756-199903170-00014},
Key = {Lester99a}
}
@article{fds132794,
Author = {DS Lester and RC Lyon and GN McGregor and RT Engelhardt and LC Schmued and GA Johnson and JN Johannessen},
Title = {3-Dimensional visualization of lesions in rat brain using
magnetic resonance imaging microscopy.},
Journal = {Neuroreport, ENGLAND},
Volume = {10},
Number = {4},
Pages = {737-41},
Year = {1999},
Month = {March},
ISSN = {0959-4965},
Keywords = {Animals • Brain • Brain Diseases •
Histocytochemistry • Image Processing,
Computer-Assisted • Kainic Acid • Magnetic
Resonance Imaging • Microscopy • Neurotoxins
• Rats • analogs & derivatives • chemically
induced • pathology • pathology* •
toxicity},
Abstract = {High-resolution (< 50 microm) magnetic resonance imaging
microscopy (MRM) has been used to identify brain regions and
localization of excitotoxin-induced lesions in fixed rat
brains, subsequently confirmed using standard histology. The
anatomical extent of lesions identified by MRM was identical
to that seen in histological sections and various
histopathological changes could be visualized. In contrast
to the time involved in preparing and examining histological
sections, lesions in intact brains could be rapidly
identified and visualized in three dimensions by examining
digitally generated sections in any plane. This study shows
that MRM has tremendous potential as a prescreening tool for
neurotoxicity and neuropathology. These observations suggest
that MRM has the potential to affect pathology much as
conventional MRI has influenced clinical
imaging.},
Key = {fds132794}
}
@article{fds132714,
Author = {G.A. Johnson},
Title = {3. J.C.M. Hsu, G.A. Johnson, W.M. Smith K.A. Reimer, R.E.
Ideker. Magnetic Resonance Imaging of Chronic Myocardial
Infarcts in Formalin Fixed Human Autopsy Specimens.
Circulation 89:2133-2140 (1993).},
Year = {1994},
Key = {fds132714}
}
@article{fds352911,
Author = {Rushmore, RJ and Wilson-Braun, P and Papadimitriou, G and Ng, I and Rathi, Y and Zhang, F and O'Donnell, LJ and Kubicki, M and Bouix, S and Yeterian, E and Lemaire, J-J and Calabrese, E and Johnson, GA and Kikinis, R and Makris, N},
Title = {3D Exploration of the Brainstem in 50-Micron Resolution
MRI.},
Journal = {Front Neuroanat},
Volume = {14},
Pages = {40},
Year = {2020},
url = {http://dx.doi.org/10.3389/fnana.2020.00040},
Abstract = {The brainstem, a structure of vital importance in mammals,
is currently becoming a principal focus in cognitive,
affective, and clinical neuroscience. Midbrain, pontine and
medullary structures serve as the conduit for signals
between the forebrain and spinal cord, are the epicenter of
cranial nerve-circuits and systems, and subserve such
integrative functions as consciousness, emotional
processing, pain, and motivation. In this study, we
parcellated the nuclear masses and the principal fiber
pathways that were visible in a high-resolution T2-weighted
MRI dataset of 50-micron isotropic voxels of a postmortem
human brainstem. Based on this analysis, we generated a
detailed map of the human brainstem. To assess the validity
of our maps, we compared our observations with histological
maps of traditional human brainstem atlases. Given the
unique capability of MRI-based morphometric analysis in
generating and preserving the morphology of 3D objects from
individual 2D sections, we reconstructed the motor, sensory
and integrative neural systems of the brainstem and rendered
them in 3D representations. We anticipate the utilization of
these maps by the neuroimaging community for applications in
basic neuroscience as well as in neurology, psychiatry, and
neurosurgery, due to their versatile computational nature in
2D and 3D representations in a publicly available
capacity.},
Doi = {10.3389/fnana.2020.00040},
Key = {fds352911}
}
@article{fds268810,
Author = {Liu, C and Li, W and Wu, B and Jiang, Y and Johnson,
GA},
Title = {3D fiber tractography with susceptibility tensor
imaging.},
Journal = {Neuroimage},
Volume = {59},
Number = {2},
Pages = {1290-1298},
Year = {2012},
Month = {January},
ISSN = {1095-9572},
url = {http://www.ncbi.nlm.nih.gov/pubmed/21867759},
Keywords = {Algorithms* • Animals • Brain • Diffusion
Tensor Imaging • Image Enhancement • Image
Interpretation, Computer-Assisted • Imaging,
Three-Dimensional • Mice • Mice, Inbred C57BL
• Nerve Fibers, Myelinated • Pattern Recognition,
Automated • Reproducibility of Results •
Sensitivity and Specificity • cytology* • methods
• methods* • ultrastructure*},
Abstract = {Gradient-echo MRI has revealed anisotropic magnetic
susceptibility in the brain white matter. This magnetic
susceptibility anisotropy can be measured and characterized
with susceptibility tensor imaging (STI). In this study, a
method of fiber tractography based on STI is proposed and
demonstrated in the mouse brain. STI experiments of
perfusion-fixed mouse brains were conducted at 7.0T. The
magnetic susceptibility tensor was calculated for each voxel
with regularization and decomposed into its eigensystem. The
major eigenvector is found to be aligned with the underlying
fiber orientation. Following the orientation of the major
eigenvector, we are able to map distinctive fiber pathways
in 3D. As a comparison, diffusion tensor imaging (DTI) and
DTI fiber tractography were also conducted on the same
specimens. The relationship between STI and DTI fiber tracts
was explored with similarities and differences identified.
It is anticipated that the proposed method of STI
tractography may provide a new way to study white matter
fiber architecture. As STI tractography is based on physical
principles that are fundamentally different from DTI, it may
also be valuable for the ongoing validation of DTI
tractography.},
Language = {eng},
Doi = {10.1016/j.neuroimage.2011.07.096},
Key = {fds268810}
}
@article{fds268850,
Author = {Driehuys, B and Walker, J and Pollaro, J and Cofer, GP and Mistry, N and Schwartz, D and Johnson, GA},
Title = {3He MRI in mouse models of asthma.},
Journal = {Magn Reson Med},
Volume = {58},
Number = {5},
Pages = {893-900},
Year = {2007},
Month = {November},
ISSN = {0740-3194},
url = {http://www.ncbi.nlm.nih.gov/pubmed/17969115},
Keywords = {Animals • Asthma • Disease Models, Animal* •
Helium • Magnetic Resonance Imaging • Mice •
Mice, Inbred BALB C • Mice, Inbred C57BL •
methods* • pathology*},
Abstract = {In the study of asthma, a vital role is played by mouse
models, because knockout or transgenic methods can be used
to alter disease pathways and identify therapeutic targets
that affect lung function. Assessment of lung function in
rodents by available methods is insensitive because these
techniques lack regional specificity. A more sensitive
method for evaluating lung function in human asthma patients
uses hyperpolarized (HP) (3)He MRI before and after
bronchoconstriction induced by methacholine (MCh). We now
report the ability to perform such (3)He imaging of MCh
response in mice, where voxels must be approximately 3000
times smaller than in humans and (3)He diffusion becomes an
impediment to resolving the airways. We show
three-dimensional (3D) images that reveal airway structure
down to the fifth branching and visualize ventilation at a
resolution of 125 x 125 x 1000 microm(3). Images of
ovalbumin (OVA)-sensitized mice acquired after MCh show both
airway closure and ventilation loss. To also observe the MCh
response in naive mice, we developed a non-slice-selective
2D protocol with 187 x 187 microm(2) resolution that was
fast enough to record the MCh response and recovery with
12-s temporal resolution. The extension of (3)He MRI to
mouse models should make it a valuable translational tool in
asthma research.},
Doi = {10.1002/mrm.21306},
Key = {fds268850}
}
@article{06059673317,
Author = {Badea, CT and Fubara, B and Hedlund, LW and Johnson,
GA},
Title = {4-D micro-CT of the mouse heart.},
Journal = {Mol Imaging},
Volume = {4},
Number = {2},
Pages = {110-116},
Year = {2005},
ISSN = {1535-3508},
url = {http://www.ncbi.nlm.nih.gov/pubmed/16105509},
Keywords = {Computerized tomography;Cardiology;Photons;Motion
control;Biological organs;Optical resolving
power;Blood;},
Abstract = {PURPOSE: Demonstrate noninvasive imaging methods for in vivo
characterization of cardiac structure and function in mice
using a micro-CT system that provides high photon fluence
rate and integrated motion control. MATERIALS AND METHODS:
Simultaneous cardiac- and respiratory-gated micro-CT was
performed in C57BL/6 mice during constant intravenous
infusion of a conventional iodinated contrast agent
(Isovue-370), and after a single intravenous injection of a
blood pool contrast agent (Fenestra VC). Multiple phases of
the cardiac cycle were reconstructed with contrast to noise
and spatial resolution sufficient for quantitative
assessment of cardiac function. RESULTS: Contrast
enhancement with Isovue-370 increased over time with a
maximum of approximately 500 HU (aorta) and 900 HU (kidney
cortex). Fenestra VC provided more constant enhancement over
3 hr, with maximum enhancement of approximately 620 HU
(aorta) and approximately 90 HU (kidney cortex). The maximum
enhancement difference between blood and myocardium in the
heart was approximately 250 HU for Isovue-370 and
approximately 500 HU for Fenestra VC. In mice with Fenestra
VC, volumetric measurements of the left ventricle were
performed and cardiac function was estimated by ejection
fraction, stroke volume, and cardiac output. CONCLUSION:
Image quality with Fenestra VC was sufficient for
morphological and functional studies required for a
standardized method of cardiac phenotyping of the
mouse.},
Doi = {10.1162/15353500200504187},
Key = {06059673317}
}
@article{fds157102,
Author = {CT Badea and B Fubara and LW Hedlund and GA Johnson},
Title = {4-D micro-CT of the mouse heart.},
Journal = {Molecular imaging : official journal of the Society for
Molecular Imaging, United States},
Volume = {4},
Number = {2},
Pages = {110-6},
ISSN = {1535-3508},
Keywords = {Animals • Contrast Media • Heart • Iodine
• Iopamidol • Mice • Mice, Inbred C57BL
• Tomography, X-Ray Computed* •
radiography*},
Abstract = {PURPOSE: Demonstrate noninvasive imaging methods for in vivo
characterization of cardiac structure and function in mice
using a micro-CT system that provides high photon fluence
rate and integrated motion control. MATERIALS AND METHODS:
Simultaneous cardiac- and respiratory-gated micro-CT was
performed in C57BL/6 mice during constant intravenous
infusion of a conventional iodinated contrast agent
(Isovue-370), and after a single intravenous injection of a
blood pool contrast agent (Fenestra VC). Multiple phases of
the cardiac cycle were reconstructed with contrast to noise
and spatial resolution sufficient for quantitative
assessment of cardiac function. RESULTS: Contrast
enhancement with Isovue-370 increased over time with a
maximum of approximately 500 HU (aorta) and 900 HU (kidney
cortex). Fenestra VC provided more constant enhancement over
3 hr, with maximum enhancement of approximately 620 HU
(aorta) and approximately 90 HU (kidney cortex). The maximum
enhancement difference between blood and myocardium in the
heart was approximately 250 HU for Isovue-370 and
approximately 500 HU for Fenestra VC. In mice with Fenestra
VC, volumetric measurements of the left ventricle were
performed and cardiac function was estimated by ejection
fraction, stroke volume, and cardiac output. CONCLUSION:
Image quality with Fenestra VC was sufficient for
morphological and functional studies required for a
standardized method of cardiac phenotyping of the
mouse.},
Key = {fds157102}
}
@article{fds132728,
Author = {G.A. Johnson},
Title = {4. X. Zhou, G.P. Cofer S. A. Suddarth, G. A. Johnson. High
Field MR Microscopy Using Fast Spin Echoes. Magn Reson Med
30:60-67 (1993).},
Year = {1994},
Key = {fds132728}
}
@article{fds268820,
Author = {Badea, CT and Johnston, SM and Qi, Y and Johnson,
GA},
Title = {4D micro-CT for cardiac and perfusion applications with view
under sampling.},
Journal = {Phys Med Biol},
Volume = {56},
Number = {11},
Pages = {3351-3369},
Year = {2011},
Month = {June},
ISSN = {1361-6560},
url = {http://www.ncbi.nlm.nih.gov/pubmed/21558587},
Keywords = {Animals • Artifacts • Four-Dimensional Computed
Tomography • Heart • Lung • Mice •
Perfusion Imaging • Phantoms, Imaging • X-Ray
Microtomography • methods* • radiography •
radiography*},
Abstract = {Micro-CT is commonly used in preclinical studies to provide
anatomical information. There is growing interest in
obtaining functional measurements from 4D micro-CT. We
report here strategies for 4D micro-CT with a focus on two
applications: (i) cardiac imaging based on retrospective
gating and (ii) pulmonary perfusion using multiple contrast
injections/rotations paradigm. A dual source micro-CT system
is used for image acquisition with a sampling rate of 20
projections per second. The cardiac micro-CT protocol
involves the use of a liposomal blood pool contrast agent.
Fast scanning of free breathing mice is achieved using
retrospective gating. The ECG and respiratory signals are
used to sort projections into ten cardiac phases. The
pulmonary perfusion protocol uses a conventional contrast
agent (Isovue 370) delivered by a micro-injector in four
injections separated by 2 min intervals to allow for
clearance. Each injection is synchronized with the rotation
of the animal, and each of the four rotations is started
with an angular offset of 22.5 from the starting angle of
the previous rotation. Both cardiac and perfusion protocols
result in an irregular angular distribution of projections
that causes significant streaking artifacts in
reconstructions when using traditional filtered
backprojection (FBP) algorithms. The reconstruction involves
the use of the point spread function of the micro-CT system
for each time point, and the analysis of the distribution of
the reconstructed data in the Fourier domain. This enables
us to correct for angular inconsistencies via deconvolution
and identify regions where data is missing. The missing
regions are filled with data from a high quality but
temporally averaged prior image reconstructed with all
available projections. Simulations indicate that
deconvolution successfully removes the streaking artifacts
while preserving temporal information. 4D cardiac micro-CT
in a mouse was performed with adequate image quality at
isotropic voxel size of 88 µm and 10 ms temporal
resolution. 4D pulmonary perfusion images were obtained in a
mouse at 176 µm and 687 ms temporal resolution. Compared
with FBP reconstruction, the streak reduction ratio is 70%
and the contrast to noise ratio is 2.5 times greater in the
deconvolved images. The radiation dose associated with the
proposed methods is in the range of a typical micro-CT dose
(0.17 Gy for the cardiac study and 0.21 Gy for the perfusion
study). The low dose 4D micro-CT imaging presented here can
be applied in high-throughput longitudinal studies in a wide
range of applications, including drug safety and
cardiopulmonary phenotyping.},
Language = {eng},
Doi = {10.1088/0031-9155/56/11/011},
Key = {fds268820}
}
@article{fds268815,
Author = {Guo, X and Johnston, SM and Qi, Y and Johnson, GA and Badea,
CT},
Title = {4D micro-CT using fast prospective gating.},
Journal = {Phys Med Biol},
Volume = {57},
Number = {1},
Pages = {257-271},
Year = {2012},
Month = {January},
ISSN = {1361-6560},
url = {http://www.ncbi.nlm.nih.gov/pubmed/22156062},
Keywords = {Animals • Cardiac-Gated Imaging Techniques •
Four-Dimensional Computed Tomography • Heart •
Mice • Phantoms, Imaging • Rats • Rats,
Sprague-Dawley • Respiratory-Gated Imaging Techniques
• Time Factors • methods* •
radiography},
Abstract = {Micro-CT is currently used in preclinical studies to provide
anatomical information. But, there is also significant
interest in using this technology to obtain functional
information. We report here a new sampling strategy for 4D
micro-CT for functional cardiac and pulmonary imaging. Rapid
scanning of free-breathing mice is achieved with fast
prospective gating (FPG) implemented on a field programmable
gate array. The method entails on-the-fly computation of
delays from the R peaks of the ECG signals or the peaks of
the respiratory signals for the triggering pulses.
Projection images are acquired for all cardiac or
respiratory phases at each angle before rotating to the next
angle. FPG can deliver the faster scan time of retrospective
gating (RG) with the regular angular distribution of
conventional prospective gating for cardiac or respiratory
gating. Simultaneous cardio-respiratory gating is also
possible with FPG in a hybrid retrospective/prospective
approach. We have performed phantom experiments to validate
the new sampling protocol and compared the results from FPG
and RG in cardiac imaging of a mouse. Additionally, we have
evaluated the utility of incorporating respiratory
information in 4D cardiac micro-CT studies with FPG. A
dual-source micro-CT system was used for image acquisition
with pulsed x-ray exposures (80 kVp, 100 mA, 10 ms). The
cardiac micro-CT protocol involves the use of a liposomal
blood pool contrast agent containing 123 mg I ml(-1)
delivered via a tail vein catheter in a dose of 0.01 ml
g(-1) body weight. The phantom experiment demonstrates that
FPG can distinguish the successive phases of phantom motion
with minimal motion blur, and the animal study demonstrates
that respiratory FPG can distinguish inspiration and
expiration. 4D cardiac micro-CT imaging with FPG provides
image quality superior to RG at an isotropic voxel size of
88 μm and 10 ms temporal resolution. The acquisition time
for either sampling approach is less than 5 min. The
radiation dose associated with the proposed method is in the
range of a typical micro-CT dose (256 mGy for the cardiac
study). Ignoring respiration does not significantly affect
anatomic information in cardiac studies. FPG can deliver
short scan times with low-dose 4D micro-CT imaging without
sacrificing image quality. FPG can be applied in
high-throughput longitudinal studies in a wide range of
applications, including drug safety and cardiopulmonary
phenotyping.},
Language = {eng},
Doi = {10.1088/0031-9155/57/1/257},
Key = {fds268815}
}
@article{fds268783,
Author = {Badea, CT and Johnston, SM and Lin, M and Hedlund, LW and Johnson,
GA},
Title = {4D micro-CT-based perfusion imaging in small
animals},
Journal = {Progress in Biomedical Optics and Imaging - Proceedings of
SPIE},
Volume = {7258},
Publisher = {SPIE},
Year = {2009},
Month = {June},
ISBN = {9780819475091},
ISSN = {1605-7422},
url = {http://dx.doi.org/10.1117/12.811213},
Abstract = {Quantitative in-vivo imaging of lung perfusion in rodents
can provide critical information for preclinical studies.
However, the combined challenges of high temporal and
spatial resolution have made routine quantitative perfusion
imaging difficult in rodents. We have recently developed a
dual tube/detector micro-CT scanner that is well suited to
capture first-pass kinetics of a bolus of contrast agent
used to compute perfusion information. Our approach is based
on the paradigm that the same time density curves can be
reproduced in a number of consecutive, small (i.e. 50μL )
injections of iodinated contrast agent at a series of
different angles. This reproducibility is ensured by the
high-level integration of the imaging components of our
system, with a micro-injector, a mechanical ventilator, and
monitoring applications. Sampling is controlled through a
biological pulse sequence implemented in LabVIEW. Image
reconstruction is based on a simultaneous algebraic
reconstruction technique implemented on a GPU. The
capabilities of 4D micro-CT imaging are demonstrated in
studies on lung perfusion in rats. We report 4D micro-CT
imaging in the rat lung with a heartbeat temporal resolution
of 140 ms and reconstructed voxels of 88 μm. The approach
can be readily extended to a wide range of important
preclinical models, such as tumor perfusion and
angiogenesis, and renal function. © 2009
SPIE.},
Doi = {10.1117/12.811213},
Key = {fds268783}
}
@article{fds268700,
Author = {Xie, L and Qi, Y and Subashi, E and Liao, G and Miller-DeGraff, L and Jetten, AM and Johnson, GA},
Title = {4D MRI of polycystic kidneys from rapamycin-treated
Glis3-deficient mice.},
Journal = {NMR Biomed},
Volume = {28},
Number = {5},
Pages = {546-554},
Year = {2015},
Month = {May},
ISSN = {0952-3480},
url = {http://dx.doi.org/10.1002/nbm.3281},
Abstract = {Polycystic kidney disease (PKD) is a life-threatening
disease that leads to a grotesque enlargement of the kidney
and significant loss of function. Several imaging studies
with MRI have demonstrated that cyst size in polycystic
kidneys can determine disease severity and progression. In
the present study, we found that, although kidney volume and
cyst volume decreased with drug treatment, renal function
did not improve with treatment. Here, we applied dynamic
contrast-enhanced MRI to study PKD in a Glis3 (GLI-similar
3)-deficient mouse model. Cysts from this model have a wide
range of sizes and develop at an early age. To capture this
crucial stage and assess cysts in detail, we imaged during
early development (3-17 weeks) and applied high
spatiotemporal resolution MRI (125 × 125 × 125 cubic
microns every 7.7 s). A drug treatment with rapamycin
(also known as sirolimus) was applied to determine whether
disease progression could be halted. The effect and synergy
(interaction) of aging and treatment were evaluated using an
analysis of variance (ANOVA). Structural measurements,
including kidney volume, cyst volume and cyst-to-kidney
volume ratio, changed significantly with age. Drug treatment
significantly decreased these metrics. Functional
measurements of time-to-peak (TTP) mean and TTP variance
were determined. TTP mean did not change with age, whereas
TTP variance increased with age. Treatment with rapamycin
generally did not affect these functional metrics.
Synergistic effects of treatment and age were not found for
any measurements. Together, the size and volume ratio of
cysts decreased with drug treatment, whereas renal function
remained the same. The quantification of renal structure and
function with MRI can comprehensively assess the
pathophysiology of PKD and response to treatment.},
Doi = {10.1002/nbm.3281},
Key = {fds268700}
}
@article{fds174275,
Author = {GA Johnson and EG Kim and SJ Boukma},
Title = {5-hydroxyindole levels in rat brain after inhibition of
dopamine -hydroxylase.},
Journal = {The Journal of pharmacology and experimental
therapeutics},
Volume = {180},
Number = {3},
Pages = {539-46},
Year = {1972},
Month = {March},
ISSN = {0022-3565},
Keywords = {Administration, Oral • Animals • Brain •
Brain Chemistry • Circadian Rhythm • Disulfiram
• Dopamine beta-Hydroxylase • Hydroxyindoleacetic
Acid • Injections, Intraperitoneal • Male •
Methyltyrosines • Mixed Function Oxygenases •
Monoiodotyrosine • Norepinephrine • Pargyline
• Rats • Rats, Inbred Strains • Serotonin
• Thiazoles • Thiourea • Time Factors •
administration & dosage • analysis • analysis*
• antagonists & inhibitors • antagonists &
inhibitors* • biosynthesis • drug effects •
enzymology* • pharmacology • pharmacology*},
Language = {eng},
Key = {fds174275}
}
@article{fds132729,
Author = {G.A. Johnson},
Title = {5. X. Zhou, Z. P. Liang, G.P. Cofer, C.F. Beaulieu, S.A.
Suddarth, G.A. Johnson. Reduction of Ringing and Blurring
Artifacts in Fast Spin-Echo Images. J Magn Reson Imag
3:803-807 (1993).},
Year = {1994},
Key = {fds132729}
}
@booklet{Hedlund86b,
Author = {Hedlund, L and Johnson, GA},
Title = {53 MAGNETIC RESONANCE IMAGING OF THE RAT THORAX AND
ABDOMEN},
Journal = {Investigative Radiology},
Volume = {21},
Number = {9},
Pages = {S14-S14},
Publisher = {Ovid Technologies (Wolters Kluwer Health)},
Year = {1986},
Month = {September},
ISSN = {0020-9996},
url = {http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:A1986E000900063&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=47d3190e77e5a3a53558812f597b0b92},
Doi = {10.1097/00004424-198609000-00071},
Key = {Hedlund86b}
}
@article{fds132715,
Author = {G.A. Johnson},
Title = {6. M.M. Henson, O.W. Henson, S.L. Gewalt, J.L. Wilson, G. A.
Johnson. Imaging the Cochlea by MRM. Hearing Research
75:75-80 (1994).},
Year = {1994},
Key = {fds132715}
}
@article{fds132730,
Author = {G.A. Johnson},
Title = {7. J. MacFall, P. Spaine, R. Doudrick, G. Johnson.
Alterations in Growth and Water Transport Processes in
Fusiform Rust Galls of Pine as Determined by Magnetic
Resonance Microsopy. Phytopathology 84:288-293
(1994).},
Year = {1994},
Key = {fds132730}
}
@booklet{Mcadams85,
Author = {MCADAMS, HP and JOHNSON, GA and SUDDARTH, SA and RAVIN,
CE},
Title = {71 HISTOGRAM DIRECTED PROCESSING OF DIGITAL CHEST
IMAGES},
Journal = {Investigative Radiology},
Volume = {20},
Number = {6},
Pages = {S18-S18},
Publisher = {Ovid Technologies (Wolters Kluwer Health)},
Year = {1985},
Month = {September},
ISSN = {0020-9996},
url = {http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:A1985ARG0500088&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=47d3190e77e5a3a53558812f597b0b92},
Doi = {10.1097/00004424-198509000-00099},
Key = {Mcadams85}
}
@article{fds132731,
Author = {G.A. Johnson},
Title = {8. B.R. Smith, G.A. Johnson, E.V. Groman, E.A. Linney.
Magnetic Resonance Microscopy of Mouse Embryos. Proc. Nat.
Acad. Sci. USA 91:3530-3533 (1994).},
Year = {1994},
Key = {fds132731}
}
@article{fds174091,
Author = {GA Johnson and AE Kimberly},
Title = {A Comparative Review of Current Methods for the
Determination of Organic Matter in Sewage.},
Journal = {Public health papers and reports},
Volume = {31},
Number = {Pt 2},
Pages = {97-108},
Year = {1905},
Language = {ENG},
Key = {fds174091}
}
@article{fds174249,
Author = {GA Johnson and AE Kimberly},
Title = {A Comparative Review of Current Methods for the
Determination of Organic Matter in Sewage.},
Journal = {Public health papers and reports},
Volume = {31},
Number = {Pt 2},
Pages = {97-108},
Year = {1905},
ISSN = {0737-8769},
Language = {eng},
Key = {fds174249}
}
@article{fds204264,
Author = {GA Johnson and JN Bloom and L Szczotka-Flynn and D Zauner and RL
Tomsak},
Title = {A comparative study of resident performance on standardized
training examinations and the american board of
ophthalmology written examination.},
Journal = {Ophthalmology},
Volume = {117},
Number = {12},
Pages = {2435-9},
Year = {2010},
Month = {December},
ISSN = {1549-4713},
url = {http://dx.doi.org/10.1016/j.ophtha.2010.03.056},
Keywords = {Adult • Certification • Clinical Competence •
Cohort Studies • Education, Medical, Graduate •
Educational Measurement* • Female • Humans •
Internship and Residency • Licensure • Male •
Ophthalmology • United States • Young Adult •
education* • standards • standards*},
Abstract = {OBJECTIVE: To investigate the relationships between
ophthalmology resident performance on the United States
Medical Licensing Examination (USMLE), the Ophthalmic
Knowledge Assessment Program (OKAP) exam and the American
Board of Ophthalmology written qualifying examination
(ABO-WQE). METHODS: Cohort study. METHODS: We included 76
residents from 15 consecutive training classes (1991-2006)
at 1 ophthalmologic residency training program. METHODS:
Numeric scores on the USMLE Step 1 and OKAP examinations
during the 3 years of residency, and first attempt pass rate
on the ABO-WQE were recorded for 76 residents. Age and
gender were also noted. Spearman's rank correlations and
univariate and multivariate logistic analyses were performed
to determine relevant associations. METHODS: First-time
attempt pass rate on the ABO-WQE and/or successful
completion of the ABO-WQE within 3 years of graduation from
the residency program. RESULTS: The ABO-WQE first-attempt
pass rate was 72.6%, consistent with the national average.
Resident USMLE scores were not significantly associated with
ABO-WQE performance. The ABO-WQE pass rate was significantly
associated with OKAP examination scores during the 3
residency years (year 1: odds ratio [OR], 8.85 and 95%
confidence interval [CI] 1.82-42.79; year 2: OR, 5.28 and
95% CI, 1.15-25.27; year 3: OR, 11.08 and 95% CI,
1.86-68.96). Passing the OKAP examinations in all 3 years
during residency training was associated with 5.43-fold
increased odds of passing the ABO-WQE and failing all 3 OKAP
examinations was associated with >9-fold lower odds of
passing the ABO-WQE on the first attempt. CONCLUSIONS: Our
results suggest that OKAP examination performance is a
predictor of a resident's success in passing the ABO-WQE on
the first attempt, as well as within 3 years of graduation
from an ophthalmologic training program. Awareness of this
association may permit identification of residents at risk
for failing the ABO-WQE and encourage educational
remediation to prevent this failure.},
Language = {eng},
Doi = {10.1016/j.ophtha.2010.03.056},
Key = {fds204264}
}
@article{fds268739,
Author = {Subashi, E and Moding, EJ and Cofer, GP and MacFall, JR and Kirsch, DG and Qi, Y and Johnson, GA},
Title = {A comparison of radial keyhole strategies for high spatial
and temporal resolution 4D contrast-enhanced MRI in small
animal tumor models.},
Journal = {Med Phys},
Volume = {40},
Number = {2},
Pages = {022304},
Year = {2013},
Month = {February},
ISSN = {0094-2405},
url = {http://www.ncbi.nlm.nih.gov/pubmed/23387766},
Abstract = {PURPOSE: Dynamic contrast-enhanced (DCE) MRI has been widely
used as a quantitative imaging method for monitoring tumor
response to therapy. The simultaneous challenges of
increasing temporal and spatial resolution in a setting
where the signal from the much smaller voxel is weaker have
made this MR technique difficult to implement in
small-animal imaging. Existing protocols employed in
preclinical DCE-MRI acquire a limited number of slices
resulting in potentially lost information in the third
dimension. This study describes and compares a family of
four-dimensional (3D spatial + time), projection
acquisition, radial keyhole-sampling strategies that support
high spatial and temporal resolution. METHODS: The 4D method
is based on a RF-spoiled, steady-state, gradient-recalled
sequence with minimal echo time. An interleaved 3D radial
trajectory with a quasi-uniform distribution of points in
k-space was used for sampling temporally resolved datasets.
These volumes were reconstructed with three different
k-space filters encompassing a range of possible radial
keyhole strategies. The effect of k-space filtering on
spatial and temporal resolution was studied in a 5 mM
CuSO(4) phantom consisting of a meshgrid with 350-μm
spacing and in 12 tumors from three cell lines (HT-29, LoVo,
MX-1) and a primary mouse sarcoma model (three
tumors∕group). The time-to-peak signal intensity was used
to assess the effect of the reconstruction filters on
temporal resolution. As a measure of heterogeneity in the
third dimension, the authors analyzed the spatial
distribution of the rate of transport (K(trans)) of the
contrast agent across the endothelium barrier for several
different types of tumors. RESULTS: Four-dimensional radial
keyhole imaging does not degrade the system spatial
resolution. Phantom studies indicate there is a maximum 40%
decrease in signal-to-noise ratio as compared to a fully
sampled dataset. T1 measurements obtained with the
interleaved radial technique do not differ significantly
from those made with a conventional Cartesian spin-echo
sequence. A bin-by-bin comparison of the distribution of the
time-to-peak parameter shows that 4D radial keyhole
reconstruction does not cause significant temporal blurring
when a temporal resolution of 9.9 s is used for the
subsamples of the keyhole data. In vivo studies reveal
substantial tumor heterogeneity in the third spatial
dimension that may be missed with lower resolution imaging
protocols. CONCLUSIONS: Volumetric keyhole imaging with
projection acquisition provides a means to increase
spatiotemporal resolution and coverage over that provided by
existing 2D Cartesian protocols. Furthermore, there is no
difference in temporal resolution between the higher spatial
resolution keyhole reconstruction and the undersampled
projection data. The technique allows one to measure complex
heterogeneity of kinetic parameters with isotropic,
microscopic spatial resolution.},
Doi = {10.1118/1.4774050},
Key = {fds268739}
}
@article{fds268786,
Author = {Guo, X and Johnston, SM and Johnson, GA and Badea,
CT},
Title = {A comparison of sampling strategies for dual energy
micro-CT},
Journal = {Progress in Biomedical Optics and Imaging - Proceedings of
SPIE},
Volume = {8313},
Publisher = {SPIE},
Year = {2012},
Month = {May},
ISBN = {9780819489623},
ISSN = {1605-7422},
url = {http://dx.doi.org/10.1117/12.911548},
Abstract = {Micro-CT has become a powerful tool for small animal
research. Many micro-CT applications require exogenous
contrast agents, which are most commonly based on iodine.
Despite advancements in contrast agents, single-energy
micro-CT is sometimes limited in the separation of two
different materials that share similar grayscale intensity
values as in the case of bone and iodine. Dual energy
micro-CT offers a solution to this separation problem, while
eliminating the need for pre-injection scanning. Various
dual energy micro-CT sampling strategies are possible,
including 1) single source sequential scanning, 2)
simultaneous dual source acquisition, or 3) single source
with kVp switching. But, no commercial micro-CT system
exists in which all these sampling strategies have been
implemented. This study reports on the implementation and
comparison of these scanning techniques on the same small
animal imaging system. Furthermore, we propose a new
sampling strategy that combines dual source and kVp
switching. Post-sampling and reconstruction, a simple
two-material dual energy decomposition was applied to
differentiate iodine from bone. The results indicate the
time differences and the potential problems associated with
each sampling strategy. Dual source scanning allows for the
fastest acquisition, but is prone to errors in decomposition
associated with scattering and imperfect geometric alignment
of the two imaging chains. KVp switching prevents these
types of artifacts, but requires more time for sampling. The
novel combination between the dual source and kVp switching
has the potential to reduce sampling time and provide better
decomposition performance. © 2012 Copyright Society of
Photo-Optical Instrumentation Engineers (SPIE).},
Doi = {10.1117/12.911548},
Key = {fds268786}
}
@article{fds174294,
Author = {TL Rutledge and SA Kamelle and TD Tillmanns and NS Gould and JD Wright and DE Cohn and TJ Herzog and JS Rader and MA Gold and GA Johnson and JL
Walker, RS Mannel and DS McMeekin},
Title = {A comparison of stages IB1 and IB2 cervical cancers treated
with radical hysterectomy. Is size the real
difference?},
Journal = {Gynecologic oncology},
Volume = {95},
Number = {1},
Pages = {70-6},
Year = {2004},
Month = {October},
ISSN = {0090-8258},
url = {http://dx.doi.org/10.1016/j.ygyno.2004.07.027},
Keywords = {Adult • Aged • Disease-Free Survival • Female
• Follow-Up Studies • Humans • Hysterectomy
• Lymph Node Excision • Lymph Nodes • Middle
Aged • Neoplasm Recurrence, Local • Neoplasm
Staging • Risk Factors • Treatment Outcome •
Uterine Cervical Neoplasms • adverse effects •
methods • pathology • pathology* • surgery
• surgery*},
Abstract = {OBJECTIVE: To compare stages IB1 and IB2 cervical cancers
treated with radical hysterectomy (RH) and to define
predictors of nodal status and recurrence. METHODS: Patients
with stage IB cervical cancers undergoing RH between 1990
and 2000 were evaluated and clinicopathological variables
were abstracted. The perioperative complication rate,
estimated blood loss (EBL), and OR time were also tabulated.
Variables were analyzed using X(2) and t tests. Disease-free
survival (DFS) was calculated by Kaplan-Meier method.
Multivariate analysis was performed via stepwise logistic
regression. Cox-proportional hazards were used to identify
independent predictors of recurrence. RESULTS: RH was
performed on 109 stage IB1 and 86 stage IB2 patients. Mean
age, EBL, and perioperative complication rates were similar.
Overall, 38 patients (14 IB1 vs. 24 IB2) had positive nodes
(P = 0.01) including 9 patients with positive para-aortic
nodes (2 IB1 and 7 IB2). Parametrial involvement (PI) and
outer 2/3 depth of invasion (DOI) were significantly more
common in the IB2 tumors as well. Patients with IB2 disease
received adjuvant radiation more frequently than IB1
patients (52% vs. 37%, P = 0.04). Univariate predictors of
nodal status included lymphovascular space involvement
(LVSI) (P = 0.001), DOI (P = 0.011), PI (P = 0.001), and
stage (P = 0.011). Multivariate analysis identified only
LVSI (OR 6.4, CI 2.4-17, P = 0. 0002) and PI (OR 8, CI
3.1-20, P = 0. 0001) as independent predictors of positive
nodes. With a median follow-up of 35 months, estimates of
DFS revealed tumor size (P = 0.008), nodal status (P =
0.0004), LVSI (P = 0.002), PI (P = 0.004), and DOI (P =
0.0004) as significant univariate predictors. Neoadjuvant
chemotherapy, age, grade, histology, and adjuvant radiation
were not associated with recurrence. The significant
independent predictors of DFS were LVSI (ROR 5.7, CI 2-16, P
= 0.0064) and outer 2/3 DOI (OR 5.8, CI 2-20, P = 0.0029).
Neither tumor size nor nodal status was a significant
predictor of DFS. CONCLUSIONS: The prognosis in stage IB
cervical cancer seems to be most influenced by presence of
LVSI and DOI and not by tumor size as the staging criteria
would suggest. These factors are best determined
pathologically after radical hysterectomy. This report
contains the largest comparison of IB1 and IB2 patients
managed by RH. Tumor size failed to predict recurrence or
nodal status when stratified by LVSI, DOI, and PI. Treatment
decisions based on tumor size alone should be
reconsidered.},
Language = {eng},
Doi = {10.1016/j.ygyno.2004.07.027},
Key = {fds174294}
}
@booklet{Murad90,
Author = {S. Murad and L. C. Walker and G. A. Johnson and S. R.
Pinnell},
Title = {A comparison of the inhibitory potential of minoxidil and
its structural analogs toward lysyl hydroxylase in cultured
fibroblasts},
Journal = {Clinical Research},
Volume = {38},
Number = {2},
Pages = {A667 -- A667},
Year = {1990},
Month = {April},
Key = {Murad90}
}
@booklet{Murad90a,
Author = {S. Murad and L. C. Walker and G. A. Johnson and S. R.
Pinnell},
Title = {A comparison of the inhibitory potential of minoxidil and
its structural analogs toward lysyl hydroxylase in cultured
fibroblasts},
Journal = {Journal Of Investigative Dermatology},
Volume = {94},
Number = {4},
Pages = {557 -- 557},
Year = {1990},
Month = {April},
Key = {Murad90a}
}
@booklet{Rogers00,
Author = {P. Rogers and P. A. Hailey and G. A. Johnson and V. A. Dight and C. Read and A. Shingler and P. Savage and T. Roche and J. Mondry},
Title = {A comprehensive and flexible approach to the
automated-dissolution testing of pharmaceutical drug
products incorporating direct UV-vis fiber-optic analysis,
on-line fluorescence analysis, and off-line storage
options},
Journal = {Laboratory Robotics And Automation},
Volume = {12},
Number = {1},
Pages = {12 -- 22},
Year = {2000},
Month = {March},
Key = {Rogers00}
}
@booklet{Hedlund81,
Author = {Hedlund, LW and Jones, DP and Effmann, EL and Johnson, GA and Bates, WM and Beck, JW and Wolfe, W and Putman, CE},
Title = {A computed tomographic study of the dog lung during
hemorrhagic shock and after resuscitation.},
Journal = {Invest Radiol},
Volume = {16},
Number = {6},
Pages = {466-472},
Year = {1981},
ISSN = {0020-9996},
url = {http://www.ncbi.nlm.nih.gov/pubmed/7319752},
Abstract = {A shock model was used to explore the capability of computed
tomography (CT) to detect changes in lung density during
hypovolemia and after resuscitation. The same level of the
lower thorax was scanned repeatedly during base-line, shock
(aortic pressure 60 mmHg), and after resuscitation with shed
blood. The average baseline CT number (+/- SEM) for 5 areas
of interest for four prone dogs was -754 +/- 16 (air =
-1000, water = 0). This decreased 7.4% to -810 +/- 15 (P
less than .05) during shock. After resuscitation CT density
was -773 +/- 17 or 2.5% less than baseline (P greater than
.1). A dorsal to ventral gradient of increasing CT density
during baseline was maintained in all five areas during
shock and post-resuscitation. From baseline to shock there
were also significant changes in heart rate, mean aortic
pressure, cardiac output, and vascular volume. Extravascular
lung volume after resuscitation was equal to baseline
volume. We conclude that CT is sufficiently sensitive to
detect rapid physiological changes leading to increased or
decreased lung density.},
Doi = {10.1097/00004424-198111000-00003},
Key = {Hedlund81}
}
@article{fds132854,
Author = {LW Hedlund and DP Jones and EL Effmann and GA Johnson and WM Bates and JW
Beck, W Wolfe and CE Putman},
Title = {A computed tomographic study of the dog lung during
hemorrhagic shock and after resuscitation.},
Journal = {Investigative radiology, UNITED STATES},
Volume = {16},
Number = {6},
Pages = {466-72},
ISSN = {0020-9996},
Keywords = {Animals • Dogs • Hemodynamic Processes • Lung
• Resuscitation* • Shock, Hemorrhagic •
Tomography, X-Ray Computed* • physiopathology •
radiography*},
Abstract = {A shock model was used to explore the capability of computed
tomography (CT) to detect changes in lung density during
hypovolemia and after resuscitation. The same level of the
lower thorax was scanned repeatedly during base-line, shock
(aortic pressure 60 mmHg), and after resuscitation with shed
blood. The average baseline CT number (+/- SEM) for 5 areas
of interest for four prone dogs was -754 +/- 16 (air =
-1000, water = 0). This decreased 7.4% to -810 +/- 15 (P
less than .05) during shock. After resuscitation CT density
was -773 +/- 17 or 2.5% less than baseline (P greater than
.1). A dorsal to ventral gradient of increasing CT density
during baseline was maintained in all five areas during
shock and post-resuscitation. From baseline to shock there
were also significant changes in heart rate, mean aortic
pressure, cardiac output, and vascular volume. Extravascular
lung volume after resuscitation was equal to baseline
volume. We conclude that CT is sufficiently sensitive to
detect rapid physiological changes leading to increased or
decreased lung density.},
Key = {fds132854}
}
@article{fds268697,
Author = {Calabrese, E and Badea, A and Cofer, G and Qi, Y and Johnson,
GA},
Title = {A Diffusion MRI Tractography Connectome of the Mouse Brain
and Comparison with Neuronal Tracer Data.},
Journal = {Cereb Cortex},
Volume = {25},
Number = {11},
Pages = {4628-4637},
Year = {2015},
Month = {November},
ISSN = {1047-3211},
url = {http://hdl.handle.net/10161/10325 Duke open
access},
Abstract = {Interest in structural brain connectivity has grown with the
understanding that abnormal neural connections may play a
role in neurologic and psychiatric diseases. Small animal
connectivity mapping techniques are particularly important
for identifying aberrant connectivity in disease models.
Diffusion magnetic resonance imaging tractography can
provide nondestructive, 3D, brain-wide connectivity maps,
but has historically been limited by low spatial resolution,
low signal-to-noise ratio, and the difficulty in estimating
multiple fiber orientations within a single image voxel.
Small animal diffusion tractography can be substantially
improved through the combination of ex vivo MRI with
exogenous contrast agents, advanced diffusion acquisition
and reconstruction techniques, and probabilistic fiber
tracking. Here, we present a comprehensive, probabilistic
tractography connectome of the mouse brain at microscopic
resolution, and a comparison of these data with a neuronal
tracer-based connectivity data from the Allen Brain Atlas.
This work serves as a reference database for future
tractography studies in the mouse brain, and demonstrates
the fundamental differences between tractography and
neuronal tracer data.},
Doi = {10.1093/cercor/bhv121},
Key = {fds268697}
}
@article{fds268695,
Author = {Calabrese, E and Badea, A and Coe, CL and Lubach, GR and Shi, Y and Styner,
MA and Johnson, GA},
Title = {A diffusion tensor MRI atlas of the postmortem rhesus
macaque brain.},
Journal = {Neuroimage},
Volume = {117},
Pages = {408-416},
Year = {2015},
Month = {August},
ISSN = {1053-8119},
url = {http://dx.doi.org/10.1016/j.neuroimage.2015.05.072},
Abstract = {The rhesus macaque (Macaca mulatta) is the most widely used
nonhuman primate for modeling the structure and function of
the brain. Brain atlases, and particularly those based on
magnetic resonance imaging (MRI), have become important
tools for understanding normal brain structure, and for
identifying structural abnormalities resulting from disease
states, exposures, and/or aging. Diffusion tensor imaging
(DTI)-based MRI brain atlases are widely used in both human
and macaque brain imaging studies because of the unique
contrasts, quantitative diffusion metrics, and diffusion
tractography that they can provide. Previous MRI and DTI
atlases of the rhesus brain have been limited by low
contrast and/or low spatial resolution imaging. Here we
present a microscopic resolution MRI/DTI atlas of the rhesus
brain based on 10 postmortem brain specimens. The atlas
includes both structural MRI and DTI image data, a detailed
three-dimensional segmentation of 241 anatomic structures,
diffusion tractography, cortical thickness estimates, and
maps of anatomic variability among atlas specimens. This
atlas incorporates many useful features from previous work,
including anatomic label nomenclature and ontology, data
orientation, and stereotaxic reference frame, and further
extends prior analyses with the inclusion of high-resolution
multi-contrast image data.},
Doi = {10.1016/j.neuroimage.2015.05.072},
Key = {fds268695}
}
@article{fds268814,
Author = {Badea, CT and Johnston, S and Johnson, B and Lin, M and Hedlund, LW and Johnson, GA},
Title = {A dual micro-CT system for small animal imaging.},
Journal = {Proc SPIE Int Soc Opt Eng},
Volume = {6913},
Pages = {691342},
Year = {2008},
Month = {April},
ISSN = {0277-786X},
url = {http://www.ncbi.nlm.nih.gov/pubmed/22049304},
Abstract = {Micro-CT is a non-invasive imaging modality usually used to
assess morphology in small animals. In our previous work, we
have demonstrated that functional micro-CT imaging is also
possible. This paper describes a dual micro-CT system with
two fixed x-ray/detectors developed to address such
challenging tasks as cardiac or perfusion studies in small
animals. A two-tube/detector system ensures simultaneous
acquisition of two projections, thus reducing scanning time
and the number of contrast injections in perfusion studies
by a factor of two. The system is integrated with software
developed in-house for cardio-respiratory monitoring and
gating. The sampling geometry was optimized for 88 microns
in such a way that the geometric blur of the focal spot
matches the Nyquist sample at the detector. A geometric
calibration procedure allows one to combine projection data
from the two chains into a single reconstructed volume.
Image quality was measured in terms of spatial resolution,
uniformity, noise, and linearity. The modulation transfer
function (MTF) at 10% is 3.4 lp/mm for single detector
reconstructions and 2.3 lp/mm for dual tube/detector
reconstructions. We attribute this loss in spatial
resolution to the compounding of slight errors in the
separate single chain calibrations. The dual micro-CT system
is currently used in studies for morphological and
functional imaging of both rats and mice.},
Language = {ENG},
Doi = {10.1117/12.772303},
Key = {fds268814}
}
@article{fds132829,
Author = {X Zhou and ZP Liang and SL Gewalt and GP Cofer and PC Lauterbur and GA
Johnson},
Title = {A fast spin echo technique with circular
sampling.},
Journal = {Magnetic resonance in medicine : official journal of the
Society of Magnetic Resonance in Medicine / Society of
Magnetic Resonance in Medicine, UNITED STATES},
Volume = {39},
Number = {1},
Pages = {23-7},
Year = {1998},
Month = {January},
ISSN = {0740-3194},
Keywords = {Animals • Brain • Echo-Planar Imaging • Image
Processing, Computer-Assisted • Magnetic Resonance
Imaging • Models, Theoretical • Phantoms, Imaging*
• Rats • Sensitivity and Specificity •
anatomy & histology* • instrumentation • methods
• methods*},
Abstract = {This paper presents a fast spin echo (FSE) imaging method
that employs circular sampling of k-space. The technique has
been implemented on a 2 Tesla imaging system and validated
on both phantoms and living animals. Experimental studies
have shown that circular sampling can produce artifact-free
FSE images without the need of phase correction. Although
not fully explored, preliminary results also show that
circular sampling may have advantages over the conventional
rectilinear FSE in signal-to-noise ratio and imaging
efficiency. A major disadvantage is the increased
sensitivity to off-resonance effects. The authors expect
that the FSE technique with circular sampling will find its
applications in magnetic resonance microscopy,
neuro-functional imaging, and real-time dynamic
studies.},
Key = {fds132829}
}
@booklet{Zhou98,
Author = {Zhou, X and Liang, ZP and Gewalt, SL and Cofer, GP and Lauterbur, PC and Johnson, GA},
Title = {A fast spin echo technique with circular
sampling.},
Journal = {Magn Reson Med},
Volume = {39},
Number = {1},
Pages = {23-27},
Year = {1998},
Month = {January},
ISSN = {0740-3194},
url = {http://www.ncbi.nlm.nih.gov/pubmed/9438433},
Abstract = {This paper presents a fast spin echo (FSE) imaging method
that employs circular sampling of k-space. The technique has
been implemented on a 2 Tesla imaging system and validated
on both phantoms and living animals. Experimental studies
have shown that circular sampling can produce artifact-free
FSE images without the need of phase correction. Although
not fully explored, preliminary results also show that
circular sampling may have advantages over the conventional
rectilinear FSE in signal-to-noise ratio and imaging
efficiency. A major disadvantage is the increased
sensitivity to off-resonance effects. The authors expect
that the FSE technique with circular sampling will find its
applications in magnetic resonance microscopy,
neuro-functional imaging, and real-time dynamic
studies.},
Doi = {10.1002/mrm.1910390106},
Key = {Zhou98}
}
@booklet{Johnson82a,
Author = {G. A. Johnson},
Title = {A generalization of n-matrices},
Journal = {Linear Algebra And Its Applications},
Volume = {48},
Number = {DEC},
Pages = {201 -- 217},
Year = {1982},
Key = {Johnson82a}
}
@article{fds268872,
Author = {de Lin, M and Ning, L and Badea, CT and Mistry, NN and Qi, Y and Johnson,
GA},
Title = {A high-precision contrast injector for small animal x-ray
digital subtraction angiography.},
Journal = {IEEE Trans Biomed Eng},
Volume = {55},
Number = {3},
Pages = {1082-1091},
Year = {2008},
Month = {March},
ISSN = {0018-9294},
url = {http://www.ncbi.nlm.nih.gov/pubmed/18334400},
Keywords = {Angiography, Digital Subtraction • Animals •
Contrast Media • Equipment Design • Equipment
Failure Analysis • Iopamidol • Mice •
Microinjections • Radiographic Image Enhancement •
Reproducibility of Results • Sensitivity and
Specificity • administration & dosage •
administration & dosage* • instrumentation* •
methods • veterinary*},
Abstract = {The availability of genetically altered animal models of
human disease for basic research has generated great
interest in new imaging methodologies. Digital subtraction
angiography (DSA) offers an appealing approach to functional
imaging in small animals because of the high spatial and
temporal resolution, and the ability to visualize and
measure blood flow. The micro-injector described here meets
crucial performance parameters to ensure optimal vessel
enhancement without significantly increasing the total blood
volume or producing overlap of enhanced structures. The
micro-injector can inject small, reproducible volumes of
contrast agent at high flow rates with computer-controlled
timing synchronized to cardiopulmonary activity. Iterative
bench-top and live animal experiments with both rat and
mouse have been conducted to evaluate the performance of
this computer-controlled micro-injector, a first
demonstration of a new device designed explicitly for the
unique requirements of DSA in small animals. Injection
protocols were optimized and screened for potential
physiological impact. For the optimized protocols, we found
that changes in the time-density curves for representative
regions of interest in the thorax were due primarily to
physiological changes, independent of micro-injector
parameters.},
Doi = {10.1109/TBME.2007.909541},
Key = {fds268872}
}
@article{fds268703,
Author = {Angeli, S and Befera, N and Peyrat, J-M and Calabrese, E and Johnson,
GA and Constantinides, C},
Title = {A high-resolution cardiovascular magnetic resonance
diffusion tensor map from ex-vivo C57BL/6 murine
hearts.},
Journal = {J Cardiovasc Magn Reson},
Volume = {16},
Number = {1},
Pages = {77},
Year = {2014},
Month = {October},
ISSN = {1097-6647},
url = {http://dx.doi.org/10.1186/s12968-014-0077-x},
Abstract = {BACKGROUND: The complex cardiac fiber structural
organization and spatial arrangement of cardiomyocytes in
laminar sheetlets contributes greatly to cardiac functional
and contractile ejection patterns. This study presents the
first comprehensive, ultra-high resolution, fully
quantitative statistical tensor map of the fixed murine
heart at isotropic resolution of 43 μm using diffusion
tensor (DT) cardiovascular magnetic resonance (CMR).
METHODS: Imaging was completed in approximately 12 hours
using a six-directional encoding scheme, in five ex vivo
healthy C57BL/6 mouse hearts. The tensor map constructed
from this data provides an average description of the murine
fiber architecture visualized with fiber tractography, and
its population variability, using the latest advances in
image tensor analysis and statistics. RESULTS: Results show
that non-normalized cardiac tensor maps are associated with
mean fractional anisotropy of 0.25 ± 0.07 and mean
diffusivity of 8.9 ± 1.6 × 10⁻⁴mm²/s. Moreover,
average mid-ventricular helical angle distributions ranged
between -41 ± 3° and +52 ± 5° and were highly
correlated with transmural depth, in agreement with prior
published results in humans and canines. Calculated
variabilities of local myocyte orientations were 2.0° and
1.4°. Laminar sheet orientation variability was found to be
less stable at 2.6°. Despite such variations, the murine
heart seems to be highly structured, particularly when
compared to canines and humans. CONCLUSIONS: This tensor map
has the potential to yield an accurate mean representation
and identification of common or unique features of the
cardiac myocyte architecture, to establish a baseline
standard reference of DTI indices, and to improve detection
of biomarkers, especially in pathological states or
post-transgenetic modifications.},
Doi = {10.1186/s12968-014-0077-x},
Key = {fds268703}
}
@article{fds356152,
Author = {Adil, SM and Calabrese, E and Charalambous, LT and Cook, JJ and Rahimpour, S and Atik, AF and Cofer, GP and Parente, BA and Johnson, GA and Lad, SP and White, LE},
Title = {A high-resolution interactive atlas of the human brainstem
using magnetic resonance imaging.},
Journal = {Neuroimage},
Volume = {237},
Pages = {118135},
Year = {2021},
Month = {August},
url = {http://dx.doi.org/10.1016/j.neuroimage.2021.118135},
Abstract = {Conventional atlases of the human brainstem are limited by
the inflexible, sparsely-sampled, two-dimensional nature of
histology, or the low spatial resolution of conventional
magnetic resonance imaging (MRI). Postmortem high-resolution
MRI circumvents the challenges associated with both
modalities. A single human brainstem specimen extending from
the rostral diencephalon through the caudal medulla was
prepared for imaging after the brain was removed from a
65-year-old male within 24 h of death. The specimen was
formalin-fixed for two weeks, then rehydrated and placed in
a custom-made MRI compatible tube and immersed in liquid
fluorocarbon. MRI was performed in a 7-Tesla scanner with
120 unique diffusion directions. Acquisition time for
anatomic and diffusion images were 14 h and 208 h,
respectively. Segmentation was performed manually.
Deterministic fiber tractography was done using
strategically chosen regions of interest and avoidance, with
manual editing using expert knowledge of human neuroanatomy.
Anatomic and diffusion images were rendered with isotropic
resolutions of 50 μm and 200 μm, respectively. Ninety
different structures were segmented and labeled, and 11
different fiber bundles were rendered with tractography. The
complete atlas is available online for interactive use at
https://www.civmvoxport.vm.duke.edu/voxbase/login.php?return_url=%2Fvoxbase%2F.
This atlas presents multiple contrasting datasets and
selected tract reconstruction with unprecedented resolution
for MR imaging of the human brainstem. There are immediate
applications in neuroanatomical education, with the
potential to serve future applications for neuroanatomical
research and enhanced neurosurgical planning through "safe"
zones of entry into the human brainstem.},
Doi = {10.1016/j.neuroimage.2021.118135},
Key = {fds356152}
}
@article{fds132839,
Author = {SE Hurlston and WW Brey and SA Suddarth and GA Johnson},
Title = {A high-temperature superconducting Helmholtz probe for
microscopy at 9.4 T.},
Journal = {Magnetic resonance in medicine : official journal of the
Society of Magnetic Resonance in Medicine / Society of
Magnetic Resonance in Medicine, UNITED STATES},
Volume = {41},
Number = {5},
Pages = {1032-8},
Year = {1999},
Month = {May},
ISSN = {0740-3194},
Keywords = {Algorithms • Aluminum Oxide • Animals •
Artifacts • Cold • Copper • Equipment Design
• Heat • Helium • Image Processing,
Computer-Assisted • Magnetic Resonance Imaging •
Mice • Microscopy • Phantoms, Imaging • Radio
Waves • Ruthenium Compounds • Sensitivity and
Specificity • Signal Processing, Computer-Assisted
• Thermal Conductivity • instrumentation •
instrumentation*},
Abstract = {The design and operation of a high-temperature
superconducting (HTS) probe for magnetic resonance
microscopy (MRM) at 400 MHz are presented. The design of the
probe includes a Helmholtz coil configuration and a stable
open-cycle cooling mechanism. Characterization of coil
operating parameters is presented to demonstrate the
suitability of cryo-cooled coils for MRM. Specifically, the
performance of the probe is evaluated by comparison of
signal-to-noise (SNR) performance with that of a copper
Helmholtz pair, analysis of B1 field homogeneity, and
quantification of thermal stability. Images are presented to
demonstrate the SNR advantage of the probe for typical MRM
applications.},
Key = {fds132839}
}
@booklet{Hurlston99,
Author = {Hurlston, SE and Brey, WW and Suddarth, SA and Johnson,
GA},
Title = {A high-temperature superconducting Helmholtz probe for
microscopy at 9.4 T.},
Journal = {Magn Reson Med},
Volume = {41},
Number = {5},
Pages = {1032-1038},
Year = {1999},
Month = {May},
ISSN = {0740-3194},
url = {http://www.ncbi.nlm.nih.gov/pubmed/10332887},
Abstract = {The design and operation of a high-temperature
superconducting (HTS) probe for magnetic resonance
microscopy (MRM) at 400 MHz are presented. The design of the
probe includes a Helmholtz coil configuration and a stable
open-cycle cooling mechanism. Characterization of coil
operating parameters is presented to demonstrate the
suitability of cryo-cooled coils for MRM. Specifically, the
performance of the probe is evaluated by comparison of
signal-to-noise (SNR) performance with that of a copper
Helmholtz pair, analysis of B1 field homogeneity, and
quantification of thermal stability. Images are presented to
demonstrate the SNR advantage of the probe for typical MRM
applications.},
Doi = {10.1002/(sici)1522-2594(199905)41:5<1032::aid-mrm23>3.0.co;2},
Key = {Hurlston99}
}
@booklet{Black93,
Author = {Black, RD and Early, TA and Roemer, PB and Mueller, OM and Mogro-Campero, A and Turner, LG and Johnson, GA},
Title = {A high-temperature superconducting receiver for nuclear
magnetic resonance microscopy.},
Journal = {Science},
Volume = {259},
Number = {5096},
Pages = {793-795},
Year = {1993},
Month = {February},
ISSN = {0036-8075},
url = {http://www.ncbi.nlm.nih.gov/pubmed/8430331},
Abstract = {A high-temperature superconducting-receiver system for use
in nuclear magnetic resonance (NMR) microscopy is described.
The scaling behavior of sources of sample and receiver-coil
noise is analyzed, and it is demonstrated that Johnson, or
thermal, noise in the receiver coil is the factor that
limits resolution. The behavior of superconductors in the
environment of an NMR experiment is examined, and a
prototypical system for imaging biological specimens is
discussed. Preliminary spin-echo images are shown, and the
ultimate limits of the signal-to-noise ratio of the probe
are investigated.},
Doi = {10.1126/science.8430331},
Key = {Black93}
}
@article{fds132865,
Author = {RD Black and TA Early and PB Roemer and OM Mueller and A Mogro-Campero and LG Turner and GA Johnson},
Title = {A high-temperature superconducting receiver for nuclear
magnetic resonance microscopy.},
Journal = {Science (New York, N.Y.), UNITED STATES},
Volume = {259},
Number = {5096},
Pages = {793-5},
Year = {1993},
Month = {February},
ISSN = {0036-8075},
Keywords = {Animals • Humans • Magnetic Resonance Spectroscopy
• Models, Theoretical • instrumentation* •
methods},
Abstract = {A high-temperature superconducting-receiver system for use
in nuclear magnetic resonance (NMR) microscopy is described.
The scaling behavior of sources of sample and receiver-coil
noise is analyzed, and it is demonstrated that Johnson, or
thermal, noise in the receiver coil is the factor that
limits resolution. The behavior of superconductors in the
environment of an NMR experiment is examined, and a
prototypical system for imaging biological specimens is
discussed. Preliminary spin-echo images are shown, and the
ultimate limits of the signal-to-noise ratio of the probe
are investigated.},
Key = {fds132865}
}
@article{fds174305,
Author = {SC Walker and T Shin and GM Zaunbrecher and JE Romano and GA Johnson and FW
Bazer, JA Piedrahita},
Title = {A highly efficient method for porcine cloning by nuclear
transfer using in vitro-matured oocytes.},
Journal = {Cloning and stem cells},
Volume = {4},
Number = {2},
Pages = {105-12},
Year = {2002},
ISSN = {1536-2302},
url = {http://dx.doi.org/10.1089/153623002320253283},
Keywords = {Animals • Cloning, Organism • Culture Techniques
• Embryo Transfer • Female • Microsatellite
Repeats • Nuclear Transfer Techniques* • Oocytes
• Pregnancy • Sex Ratio • Swine •
cytology* • genetics • genetics* •
methods*},
Abstract = {To date, the efficiency of pig cloning by nuclear transfer
of somatic cell nuclei has been extremely low, with less
than 1% of transferred embryos surviving to term. Even the
utilization of complex procedures such as two rounds of
nuclear transfer has not resulted in greater overall
efficiencies. As a result, the applicability of the
technology for the generation of transgenic and cloned
animals has not moved forward rapidly. We report here a
simple nuclear transfer protocol, utilizing commercially
available in vitro-matured oocytes, that results in greater
than 5% overall cloning efficiency. Of five recipients
receiving nuclear transfer embryos produced with a fetal
fibroblast cell line as nuclear donor, all five established
pregnancies by day 28 (100%), and 4/5 (80%) went to term.
Efficiencies for each transfer were 7% (9 piglets/128
doublets transferred), 5% (5/100), 12% (7/59), and 6.6%
(7/106). The overall efficiency in all recipients was 5.5%
and in pregnant recipients 7.7%, with a total of 28 cloned
piglets produced. With the average fusion rate being 58%,
the percentage of fused doublets producing a live piglet
approached 12%. The method described here can be undertaken
by a single micromanipulator at a reasonable cost, and
should facilitate the broad utilization of porcine cloning
technology in transgenic and nontransgenic
applications.},
Language = {eng},
Doi = {10.1089/153623002320253283},
Key = {fds174305}
}
@article{fds174077,
Author = {DJ Waldon and MF Kubicek and GA Johnson and AE Buhl},
Title = {A HPLC-based chloramphenicol acetyltransferase assay for
assessing hair growth: comparison of the sensitivity of UV
and fluorescence detection.},
Journal = {European journal of clinical chemistry and clinical
biochemistry : journal of the Forum of European Clinical
Chemistry Societies},
Volume = {31},
Number = {1},
Pages = {41-5},
Year = {1993},
Month = {January},
ISSN = {0939-4974},
Keywords = {Animals • Chloramphenicol O-Acetyltransferase •
Chromatography, High Pressure Liquid* • Culture
Techniques • Gene Expression • Hair •
Keratins • Mice • Mice, Transgenic • Promoter
Regions, Genetic • Sensitivity and Specificity •
Spectrometry, Fluorescence • Spectrophotometry,
Ultraviolet • analysis* • genetics •
genetics* • growth & development*},
Abstract = {In our attempt to measure hair growth by hair-specific
markers, we used transgenic mice to express the
chloramphenicol acetyltransferase gene under the control of
an ultrahigh sulphur keratin gene promoter. To quantitate
expression of the keratin gene, we required a
chloramphenicol acetyltransferase assay which could measure
enzyme activity in a single follicle and also could be used
to assay a large number of samples without loss of
sensitivity. We achieved this objective by utilizing a
fluorescent substrate for chloramphenicol acetyltransferase.
With HPLC-fluorescence detection, this substrate provides a
sensitivity of less than 1 x 10(-13) mol, which is 1000
times greater than that achievable with HPLC-UV detection in
cultured follicles. Further, the assay was automated to
facilitate the analysis of more than 100 samples/day. It
should be possible to apply this fluorescent assay to a
number of cell or tissue studies.},
Language = {eng},
Key = {fds174077}
}
@booklet{Waldon93a,
Author = {D. J. Waldon and M. F. Kubicek and G. A. Johnson and A. E.
Buhl},
Title = {A hplc-based chloramphenicol acetyltransferase assay for
assessing hair-growth - comparison of the sensitivity of uv
and fluorescence detection},
Journal = {European Journal Of Clinical Chemistry And Clinical
Biochemistry},
Volume = {31},
Number = {1},
Pages = {41 -- 45},
Year = {1993},
Month = {January},
Key = {Waldon93a}
}
@article{fds312813,
Author = {Badea, CT and Hedlund, LW and Johnson, GA},
Title = {A LabVIEW Platform for Preclinical Imaging Using Digital
Subtraction Angiography and Micro-CT.},
Journal = {J Med Eng},
Volume = {2013},
Number = {581617},
Pages = {581617},
Year = {2013},
url = {http://hdl.handle.net/10161/11992 Duke open
access},
Abstract = {CT and digital subtraction angiography (DSA) are ubiquitous
in the clinic. Their preclinical equivalents are valuable
imaging methods for studying disease models and treatment.
We have developed a dual source/detector X-ray imaging
system that we have used for both micro-CT and DSA studies
in rodents. The control of such a complex imaging system
requires substantial software development for which we use
the graphical language LabVIEW (National Instruments,
Austin, TX, USA). This paper focuses on a LabVIEW platform
that we have developed to enable anatomical and functional
imaging with micro-CT and DSA. Our LabVIEW applications
integrate and control all the elements of our system
including a dual source/detector X-ray system, a mechanical
ventilator, a physiological monitor, and a power
microinjector for the vascular delivery of X-ray contrast
agents. Various applications allow cardiac- and
respiratory-gated acquisitions for both DSA and micro-CT
studies. Our results illustrate the application of DSA for
cardiopulmonary studies and vascular imaging of the liver
and coronary arteries. We also show how DSA can be used for
functional imaging of the kidney. Finally, the power of 4D
micro-CT imaging using both prospective and retrospective
gating is shown for cardiac imaging.},
Doi = {10.1155/2013/581617},
Key = {fds312813}
}
@article{fds268870,
Author = {Mukundan, S and Ghaghada, KB and Badea, CT and Kao, C-Y and Hedlund, LW and Provenzale, JM and Johnson, GA and Chen, E and Bellamkonda, RV and Annapragada, A},
Title = {A liposomal nanoscale contrast agent for preclinical CT in
mice.},
Journal = {AJR Am J Roentgenol},
Volume = {186},
Number = {2},
Pages = {300-307},
Year = {2006},
Month = {February},
ISSN = {0361-803X},
url = {http://www.ncbi.nlm.nih.gov/pubmed/16423931},
Keywords = {Animals • Contrast Media • Image Processing,
Computer-Assisted • Liposomes • Mice •
Radiography, Thoracic • Tomography, X-Ray Computed*
• Triiodobenzoic Acids • chemistry •
pharmacokinetics*},
Abstract = {OBJECTIVE: The goal of this study was to determine if an
iodinated, liposomal contrast agent could be used for
high-resolution, micro-CT of low-contrast, small-size
vessels in a murine model. MATERIALS AND METHODS: A
second-generation, liposomal blood pool contrast agent
encapsulating a high concentration of iodine (83-105 mg
I/mL) was evaluated. A total of five mice weighing between
20 and 28 g were infused with equivalent volume doses (500
microL of contrast agent/25 g of mouse weight) and imaged
with our micro-CT system for intervals of up to 240 min
postinfusion. The animals were anesthetized, mechanically
ventilated, and vital signs monitored allowing for
simultaneous cardiac and respiratory gating of image
acquisition. RESULTS: Initial enhancement of about 900 H in
the aorta was obtained, which decreased to a plateau level
of approximately 800 H after 2 hr. Excellent contrast
discrimination was shown between the myocardium and cardiac
blood pool (650-700 H). No significant nephrogram was
identified, indicating the absence of renal clearance of the
agent. CONCLUSION: The liposomal-based iodinated contrast
agent shows long residence time in the blood pool, very high
attenuation within submillimeter vessels, and no significant
renal clearance rendering it an effective contrast agent for
murine vascular imaging using a micro-CT
scanner.},
Doi = {10.2214/AJR.05.0523},
Key = {fds268870}
}
@article{fds268784,
Author = {Johnston, SM and Johnson, GA and Badea, CT},
Title = {A material decomposition method for dual energy
micro-CT},
Journal = {Progress in Biomedical Optics and Imaging - Proceedings of
SPIE},
Volume = {7258},
Publisher = {SPIE},
Year = {2009},
Month = {June},
ISBN = {9780819475091},
ISSN = {1605-7422},
url = {http://dx.doi.org/10.1117/12.811673},
Abstract = {The attenuation of x-rays in matter is dependent on the
energy of the x-rays and the atomic composition of the
matter. Attenuation measurements at multiple x-ray energies
can be used to improve the identification of materials. We
present a method to estimate the fractional composition of
three materials in an object from x-ray CT measurements at
two different energies. The energies can be collected from
measurements from a single source-detector system at two
points in time, or from a dual source-detector system at one
point in time. This method sets up a linear system of
equations from the measurements and finds the solution
through a geometric construction of the inverse matrix
equation. This method enables the estimation of the blood
fraction within a region of living tissue in which blood
containing an iodinated contrast agent is mixed with two
other materials. We verified this method using x-ray CT
simulations implemented in MATLAB, investigated the
parameters needed to optimize the estimation, and then
applied the method to a mouse model of lung cancer. A direct
application of this method is the estimation of blood
fraction in lung tumors in preclinical studies. This work
was performed at the Duke Center for In Vivo Microscopy, an
NCRR/NCI National Resource (P41 RR005959/U24 CA092656), and
also supported by NCI R21 CA124584. ©2009
SPIE.},
Doi = {10.1117/12.811673},
Key = {fds268784}
}
@article{fds359549,
Author = {Feinberg, DA and Johnson, G},
Title = {A methodology for co-registering abdominal MR images over
multiple breath-holds.},
Journal = {Magn Reson Med},
Volume = {34},
Number = {5},
Pages = {770-774},
Year = {1995},
Month = {November},
url = {http://dx.doi.org/10.1002/mrm.1910340517},
Abstract = {Previous studies have demonstrated that the SNR of abdominal
MR images can be increased by averaging images obtained in
different breath-hold acquisitions. In this note, the
authors present a simple new methodology for ensuring that
images acquired in multiple breath-hold periods are
accurately co-registered. Within each breath-hold, a quick
coronal scout scan is followed by a longer axial scan. The
scout is used to position the axial slices in a fixed
position relative to the organ under examination. This MR
technique can, in principle, be automated so as to add less
than 1 s to the imaging time of the axial scan. The method
can be used to increase SNR by signal averaging or to
co-register images acquired during, for example, uptake of
contrast agents. SNR improvement with negligible blurring is
demonstrated in liver images acquired by this method from
healthy volunteers.},
Doi = {10.1002/mrm.1910340517},
Key = {fds359549}
}
@article{fds268847,
Author = {Shofer, S and Badea, C and Auerbach, S and Schwartz, DA and Johnson,
GA},
Title = {A micro-computed tomography-based method for the measurement
of pulmonary compliance in healthy and bleomycin-exposed
mice.},
Journal = {Exp Lung Res},
Volume = {33},
Number = {3-4},
Pages = {169-183},
Year = {2007},
ISSN = {0190-2148},
url = {http://www.ncbi.nlm.nih.gov/pubmed/17558678},
Keywords = {Animals • Bleomycin • Disease Models, Animal
• Female • Image Processing, Computer-Assisted
• Lung • Lung Compliance* • Male • Mice
• Mice, Inbred C57BL • Pulmonary Fibrosis •
Reproducibility of Results • Time Factors •
Tomography, X-Ray Computed • chemically induced •
methods* • pathology • physiopathology •
radiography*},
Abstract = {Micro-computed tomography (microCT) is being increasingly
used to examine small animal models of pulmonary injury. The
authors have developed a microCT technique suitable for the
determination of pulmonary compliance in injured mice. Lung
volumes in normal mice were radiographically determined at
end-inspiration and end-expiration and pulmonary compliance
was calculated at 2 time points 2 weeks apart, whereas a
second group of mice were given bleomycin and imaged 3 weeks
following drug administration. Compliance measurements were
validated using a commercially available ventilator system.
MicroCT pulmonary compliance measurements are suitable for
longitudinal measurements, and correlate with physiologic
measurements of pulmonary compliance.},
Doi = {10.1080/01902140701364458},
Key = {fds268847}
}
@article{fds268854,
Author = {Shofer, S and Badea, C and Qi, Y and Potts, E and Foster, WM and Johnson,
GA},
Title = {A micro-CT analysis of murine lung recruitment in
bleomycin-induced lung injury.},
Journal = {J Appl Physiol (1985)},
Volume = {105},
Number = {2},
Pages = {669-677},
Year = {2008},
Month = {August},
ISSN = {8750-7587},
url = {http://www.ncbi.nlm.nih.gov/pubmed/18566189},
Keywords = {Animals • Antibiotics, Antineoplastic* •
Bleomycin* • Collagen • Female • Image
Processing, Computer-Assisted • Lung Compliance •
Lung Diseases • Mice • Pulmonary Alveoli •
Respiratory Function Tests • Respiratory Mechanics
• Tidal Volume • Tomography, X-Ray Computed •
chemically induced* • metabolism • pathology
• pathology* • physiology •
physiopathology},
Abstract = {The effects of lung injury on pulmonary recruitment are
incompletely understood. X-ray computed tomography (CT) has
been a valuable tool in assessing changes in recruitment
during lung injury. With the development of preclinical CT
scanners designed for thoracic imaging in rodents, it is
possible to acquire high-resolution images during the
evolution of a pulmonary injury in living mice. We
quantitatively assessed changes in recruitment caused by
intratracheal bleomycin at 1 and 3 wk after administration
using micro-CT in 129S6/SvEvTac mice. Twenty female mice
were administered 2.5 U of bleomycin or saline and imaged
with micro-CT at end inspiration and end expiration. Mice
were extubated and allowed to recover from anesthesia and
then reevaluated in vivo for quasi-static compliance
measurements, followed by harvesting of the lungs for
collagen analysis and histology. CT images were converted to
histograms and analyzed for mean lung attenuation (MLA). MLA
was significantly greater for bleomycin-exposed mice at week
1 for both inspiration (P<0.0047) and exhalation (P<0.0377)
but was not significantly different for week 3
bleomycin-exposed mice. However, week 3 bleomycin-exposed
mice did display significant increases in MLA shift from
expiration to inspiration compared with either group of
control mice (P<0.005), suggesting increased lung
recruitment at this time point. Week 1 bleomycin-exposed
mice displayed normal shifts in MLA with inspiration,
suggesting normal lung recruitment despite significant
radiographic and histological changes. Lung alveolar
recruitment is preserved in a mouse model of
bleomycin-induced parenchymal injury despite significant
changes in radiographic and physiological
parameters.},
Doi = {10.1152/japplphysiol.00980.2007},
Key = {fds268854}
}
@booklet{Johnson92b,
Author = {G. A. Johnson and H. S. Borovetz and J. L.
Anderson},
Title = {A model of pulsatile flow in a uniform deformable
vessel},
Journal = {Journal Of Biomechanics},
Volume = {25},
Number = {1},
Pages = {91 -- 100},
Year = {1992},
Month = {January},
Key = {Johnson92b}
}
@article{fds174289,
Author = {GA Johnson and HS Borovetz and JL Anderson},
Title = {A model of pulsatile flow in a uniform deformable
vessel.},
Journal = {Journal of biomechanics},
Volume = {25},
Number = {1},
Pages = {91-100},
Year = {1992},
Month = {January},
ISSN = {0021-9290},
Keywords = {Animals • Blood Circulation • Blood Flow Velocity
• Blood Pressure • Blood Vessels • Carotid
Arteries • Dogs • Models, Cardiovascular* •
Movement • Regional Blood Flow • Rheology •
Stress, Mechanical • anatomy & histology •
physiology • physiology*},
Abstract = {Simulations of blood flow in natural and artificial conduits
usually require large computers for numerical solution of
the Navier-Stokes equations. Often, physical insight into
the fluid dynamics is lost when the solution is purely
numerical. An alternative to solving the most general form
of the Navier-Stokes equations is described here, wherein a
functional form of the solution is assumed in order to
simplify the required computations. The assumed forms for
the axial pressure gradient and velocity profile are chosen
such that conservation of mass is satisfied for fully
established pulsatile flow in a straight, deformable vessel.
The resulting equations are cast in finite-difference form
and solved explicitly. Results for the limiting cases of
rigid wall and zero applied pressure are found to be in good
agreement with analytical solutions. Comparison with the
experimental results of Klanchar et al. [Circ. Res. 66,
1624-1635 (1990]) also shows good agreement. Application of
the model to realistic physiological parameter values
provides insight as to the influence of the pulsatile nature
of the flow field on wall shear development in the presence
of a moving wall boundary. Specifically, the model
illustrates the dependence of flow rate and shear rate on
the amplitude of the vessel wall motion and the phase
difference between the applied pressure difference and the
oscillations of the vessel radius. The present model can
serve as a useful tool for experimentalists interested in
quantifying the magnitude and character of velocity profiles
and shearing forces in natural and artificial biologic
conduits.},
Language = {eng},
Key = {fds174289}
}
@article{fds268709,
Author = {Clark, DP and Johnson, GA and Badea, CT},
Title = {A multi-resolution approach to retrospectively-gated cardiac
micro-CT reconstruction},
Journal = {Progress in Biomedical Optics and Imaging - Proceedings of
SPIE},
Volume = {9033},
Publisher = {SPIE},
Year = {2014},
Month = {January},
ISBN = {9780819498267},
ISSN = {1605-7422},
url = {http://dx.doi.org/10.1117/12.2043044},
Abstract = {In preclinical research, micro-CT is commonly used to
provide anatomical information; however, there is
significant interest in using this technology to obtain
functional information in cardiac studies. The fastest
acquisition in 4D cardiac micro-CT imaging is achieved via
retrospective gating, resulting in irregular angular
projections after binning the projections into phases of the
cardiac cycle. Under these conditions, analytical
reconstruction algorithms, such as filtered back projection,
suffer from streaking artifacts. Here, we propose a novel,
multi-resolution, iterative reconstruction algorithm
inspired by robust principal component analysis which
prevents the introduction of streaking artifacts, while
attempting to recover the highest temporal resolution
supported by the projection data. The algorithm achieves
these results through a unique combination of the split
Bregman method and joint bilateral filtration. We illustrate
the algorithm’s performance using a
contrast-enhanced, 2D slice through the MOBY mouse phantom
and realistic projection acquisition and reconstruction
parameters. Our results indicate that the algorithm is
robust to under sampling levels of only 34 projections per
cardiac phase and, therefore, has high potential in reducing
both acquisition times and radiation dose. Another potential
advantage of the multi-resolution scheme is the natural
division of the reconstruction problem into a large number
of independent sub-problems which can be solved in parallel.
In future work, we will investigate the performance of this
algorithm with retrospectively-gated, cardiac micro-CT data.
© 2014 SPIE.},
Doi = {10.1117/12.2043044},
Key = {fds268709}
}
@article{fds358783,
Author = {Johnson, GA and Laoprasert, R and Anderson, RJ and Cofer, G and Cook, J and Pratson, F and White, LE},
Title = {A multicontrast MR atlas of the Wistar rat
brain.},
Journal = {Neuroimage},
Volume = {242},
Pages = {118470},
Year = {2021},
Month = {November},
url = {http://dx.doi.org/10.1016/j.neuroimage.2021.118470},
Abstract = {We describe a multi-contrast, multi-dimensional atlas of the
Wistar rat acquired at microscopic spatial resolution using
magnetic resonance histology (MRH). Diffusion weighted
images, and associated scalar images were acquired of a
single specimen with a fully sampled Fourier reconstruction,
61 angles and b=3000 s/mm2 yielding 50 um isotropic spatial
resolution. The higher angular sampling allows use of the
GQI algorithm improving the angular invariance of the scalar
images and yielding an orientation distribution function to
assist in delineating subtle boundaries where there are
crossing fibers and track density images providing insight
into local fiber architecture. A multigradient echo image
of the same specimen was acquired at 25 um isotropic spatial
resolution. A quantitative susceptibility map enhances fiber
architecture relative to the magnitude images. An
accompanying multi-specimen atlas (n=6) was acquired with
compressed sensing with the same diffusion protocol as used
for the single specimen atlas. An average was created
using diffeomorphic mapping. Scalar volumes from the
diffusion data, a T2* weighted volume, a quantitative
susceptibility map, and a track density volume, all
registered to the same space provide multiple contrasts to
assist in anatomic delineation. The new template provides
significantly increased contrast in the scalar DTI images
when compared to previous atlases. A compact interactive
viewer based on 3D Slicer is provided to facilitate
comparison among the contrasts in the multiple volumes. The
single volume and average atlas with multiple 3D volumes
provide an improved template for anatomic interrogation of
the Wistar rat brain. The improved contrast to noise in the
scalar DTI images and the addition of other volumes (eg.
QA,QSM,TDI ) will facilitate automated label registration
for MR histology and preclinical imaging.},
Doi = {10.1016/j.neuroimage.2021.118470},
Key = {fds358783}
}
@article{fds268795,
Author = {Johnson, GA and Calabrese, E and Badea, A and Paxinos, G and Watson,
C},
Title = {A multidimensional magnetic resonance histology atlas of the
Wistar rat brain.},
Journal = {Neuroimage},
Volume = {62},
Number = {3},
Pages = {1848-1856},
Year = {2012},
Month = {September},
url = {http://www.ncbi.nlm.nih.gov/pubmed/22634863},
Abstract = {We have produced a multidimensional atlas of the adult
Wistar rat brain based on magnetic resonance histology
(MRH). This MR atlas has been carefully aligned with the
widely used Paxinos-Watson atlas based on optical sections
to allow comparisons between histochemical and immuno-marker
data, and the use of the Paxinos-Watson abbreviation set.
Our MR atlas attempts to make a seamless connection with the
advantageous features of the Paxinos-Watson atlas, and to
extend the utility of the data through the unique
capabilities of MR histology: a) ability to view the brain
in the skull with limited distortion from shrinkage or
sectioning; b) isotropic spatial resolution, which permits
sectioning along any arbitrary axis without loss of detail;
c) three-dimensional (3D) images preserving spatial
relationships; and d) widely varied contrast dependent on
the unique properties of water protons. 3D diffusion tensor
images (DTI) at what we believe to be the highest resolution
ever attained in the rat provide unique insight into white
matter structures and connectivity. The 3D isotropic data
allow registration of multiple data sets into a common
reference space to provide average atlases not possible with
conventional histology. The resulting multidimensional atlas
that combines Paxinos-Watson with multidimensional MRH
images from multiple specimens provides a new, comprehensive
view of the neuroanatomy of the rat and offers a
collaborative platform for future rat brain
studies.},
Doi = {10.1016/j.neuroimage.2012.05.041},
Key = {fds268795}
}
@article{fds132832,
Author = {WL Hall and H Benveniste and LW Hedlund and GA Johnson},
Title = {A new in vivo method for quantitative analysis of stroke
lesions using diffusion-weighted magnetic resonance
microscopy.},
Journal = {NeuroImage, UNITED STATES},
Volume = {3},
Number = {3 Pt 1},
Pages = {158-66},
Year = {1996},
Month = {June},
ISSN = {1053-8119},
Keywords = {Animals • Brain Ischemia • Disease Models, Animal
• Female • Magnetic Resonance Imaging* •
Microscopy • Models, Neurological • Rats •
Rats, Inbred F344 • Stroke Volume • diagnosis
• methods* • pathology* •
physiopathology},
Abstract = {Using three-dimensional diffusion-weighted MR microscopy and
a rat model of focal cerebral ischemia, we evaluated the
statistical characteristics of two parameters: absolute
stroke volumes and change in stroke volumes over 6 h of
middle cerebral artery (MCA) occlusion. In all rats, the
absolute stroke volumes increased linearly over the 6-h MCA
occlusion time period. On average, stroke volume growth rate
was 2.1 +/- 0.5%/h. Sample size power analysis of our data
demonstrated that to demonstrate a 10% reduction of the 6-h
volumes, sample size per group would require 29 animals
(these calculations are based on alpha = 0.05, beta = 0.20
using normal approximation). A similar 30% reduction of
stroke volume at 6 h poststroke (from approximately equal to
200 to 140 mm3) would, in our "slope model," translate into
a reduction of stroke growth rate from the normal + 11.25
mm3/h (150 to 200 mm3 over 4 h) to 7 mm3/h (150 to 178 mm3
over 4 h); power analysis in this case demonstrated that
sample size is reduced to 15 animals per group (these
calculations are based on alpha = 0.05, beta = 0.20 using
normal approximation). We conclude that from a statistical
standpoint our study demonstrates that stroke growth rate
might be a more suitable parameter for evaluating the effect
of treatment in both clinical and experimental stroke
trials.},
Key = {fds132832}
}
@booklet{Hall96,
Author = {Hall, WL and Benveniste, H and Hedlund, LW and Johnson,
GA},
Title = {A new in vivo method for quantitative analysis of stroke
lesions using diffusion-weighted magnetic resonance
microscopy.},
Journal = {Neuroimage},
Volume = {3},
Number = {3 Pt 1},
Pages = {158-166},
Year = {1996},
Month = {June},
ISSN = {1053-8119},
url = {http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:A1996UQ76900002&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=47d3190e77e5a3a53558812f597b0b92},
Abstract = {Using three-dimensional diffusion-weighted MR microscopy and
a rat model of focal cerebral ischemia, we evaluated the
statistical characteristics of two parameters: absolute
stroke volumes and change in stroke volumes over 6 h of
middle cerebral artery (MCA) occlusion. In all rats, the
absolute stroke volumes increased linearly over the 6-h MCA
occlusion time period. On average, stroke volume growth rate
was 2.1 +/- 0.5%/h. Sample size power analysis of our data
demonstrated that to demonstrate a 10% reduction of the 6-h
volumes, sample size per group would require 29 animals
(these calculations are based on alpha = 0.05, beta = 0.20
using normal approximation). A similar 30% reduction of
stroke volume at 6 h poststroke (from approximately equal to
200 to 140 mm3) would, in our "slope model," translate into
a reduction of stroke growth rate from the normal + 11.25
mm3/h (150 to 200 mm3 over 4 h) to 7 mm3/h (150 to 178 mm3
over 4 h); power analysis in this case demonstrated that
sample size is reduced to 15 animals per group (these
calculations are based on alpha = 0.05, beta = 0.20 using
normal approximation). We conclude that from a statistical
standpoint our study demonstrates that stroke growth rate
might be a more suitable parameter for evaluating the effect
of treatment in both clinical and experimental stroke
trials.},
Doi = {10.1006/nimg.1996.0017},
Key = {Hall96}
}
@booklet{Johnson98,
Author = {Allan Johnson and G and Hedlund, L and MacFall, J},
Title = {A new window into the lung},
Journal = {Physics World},
Volume = {11},
Number = {11},
Pages = {35-38},
Publisher = {IOP Publishing},
Year = {1998},
Month = {January},
ISSN = {0953-8585},
url = {http://dx.doi.org/10.1088/2058-7058/11/11/28},
Doi = {10.1088/2058-7058/11/11/28},
Key = {Johnson98}
}
@booklet{Todd01,
Author = {M. D. Todd and G. A. Johnson and B. L. Althouse},
Title = {A novel Bragg grating sensor interrogation system utilizing
a scanning filter, a Mach-Zehnder interferometer and a 3 x 3
coupler},
Journal = {Measurement Science \& Technology},
Volume = {12},
Number = {7},
Pages = {771 -- 777},
Year = {2001},
Month = {July},
Key = {Todd01}
}
@article{fds161597,
Author = {G.A. Johnson},
Title = {A Petiet, GA Johnson, Active Staining of Mouse Embryos for
Magnetic Resonance Microscopy. In: Hewitson Tim D & Darby
Ian A (editors), Histology Protocols (Methods in Molecular
Biology), Springer- Humana Press. Totowa, NJ. USA, in press,
2009},
Year = {2009},
Key = {fds161597}
}
@booklet{Godwin84a,
Author = {Godwin, JD and Johnson, GA and Fram, EK},
Title = {A phantom for testing ECG-gated computed tomography of the
heart.},
Journal = {Invest Radiol},
Volume = {19},
Number = {4},
Pages = {279-283},
Year = {1984},
ISSN = {0020-9996},
url = {http://www.ncbi.nlm.nih.gov/pubmed/6480305},
Abstract = {A mechanical phantom has been built to evaluate
electrocardiographically gated computed tomography of the
heart. The phantom simulates the heart in terms of cyclic
changes in chamber dimensions and wall thickness. Rate and
excursion are variable, and the cavity of the chamber can be
filled with liquid contrast media of different degrees of
radio-opacity. Preliminary experiments with a prototypic
gating system are described.},
Doi = {10.1097/00004424-198407000-00008},
Key = {Godwin84a}
}
@article{fds132753,
Author = {JD Godwin and GA Johnson and EK Fram},
Title = {A phantom for testing ECG-gated computed tomography of the
heart.},
Journal = {Investigative radiology, UNITED STATES},
Volume = {19},
Number = {4},
Pages = {279-83},
ISSN = {0020-9996},
Keywords = {Electrocardiography* • Heart • Humans •
Models, Structural* • Myocardial Contraction •
Tomography, X-Ray Computed • methods* •
radiography*},
Abstract = {A mechanical phantom has been built to evaluate
electrocardiographically gated computed tomography of the
heart. The phantom simulates the heart in terms of cyclic
changes in chamber dimensions and wall thickness. Rate and
excursion are variable, and the cavity of the chamber can be
filled with liquid contrast media of different degrees of
radio-opacity. Preliminary experiments with a prototypic
gating system are described.},
Key = {fds132753}
}
@booklet{Look98,
Author = {K. Y. Look and J. A. Blessing and B. E. Nelson and G. A.
Johnson and W. C. Fowler and G. C. Reid},
Title = {A phase II trial of isotretinoin and alpha interferon in
patients with recurrent squamous cell carcinoma of the
cervix - A Gynecologic Oncology Group Study},
Journal = {American Journal Of Clinical Oncology-cancer Clinical
Trials},
Volume = {21},
Number = {6},
Pages = {591 -- 594},
Year = {1998},
Month = {December},
Key = {Look98}
}
@article{fds174221,
Author = {KY Look and JA Blessing and BE Nelson and GA Johnson and WC Fowler Jr and GC Reid},
Title = {A phase II trial of isotretinoin and alpha interferon in
patients with recurrent squamous cell carcinoma of the
cervix: a Gynecologic Oncology Group study.},
Journal = {American journal of clinical oncology},
Volume = {21},
Number = {6},
Pages = {591-4},
Year = {1998},
Month = {December},
ISSN = {0277-3732},
Keywords = {Adult • Aged • Antineoplastic Agents •
Carcinoma, Squamous Cell • Drug Therapy, Combination
• Female • Humans • Interferon-alpha •
Isotretinoin • Keratolytic Agents • Middle Aged
• Neoplasm Recurrence, Local • Uterine Cervical
Neoplasms • administration & dosage • drug
therapy* • therapeutic use*},
Abstract = {From January 1993 through January 1996, 37 patients with
unresectable squamous carcinoma of the cervix were entered
on study and scheduled to receive oral isotretinoin 1 mg/kg
per day with subcutaneous alpha interferon 6,000,000
units/day. A course was defined as 4 continuous weeks of
therapy. The mean number of four-course cycles delivered was
1.8. One patient was ineligible because of wrong cell type
and two were never treated. Thus, 34 patients were evaluable
for toxicity. Eight patients were inevaluable for response.
Five did not receive a complete 4-week course and three did
not have additional tumor measurements; thus 26 were
evaluable for response. Prior radiotherapy had been given to
25 patients and prior chemotherapy to 23 patients. There was
no grade 4 neutropenia. The incidence of Gynecologic
Oncology Group (GOG) grade 3 granulocytopenia and
thrombocytopenia was 8.8% and 5.8%, respectively. Six
patients (17.6%) developed grade 3 or worse nausea and
vomiting. Four (11.7%) patients developed grade 3 neurologic
symptoms. There were no complete responses and one partial
response. The overall response rate was 3.8% (95% confidence
interval, 0.1-19.6%). In this pretreated population,
isotretinoin and alpha interferon in the dose and schedule
employed exhibit minimal activity.},
Language = {eng},
Key = {fds174221}
}
@booklet{Johnson82f,
Author = {JOHNSON, GA},
Title = {A PRACTICAL APPROACH TO QUALITY ASSURANCE IN
CT},
Journal = {AMERICAN JOURNAL OF ROENTGENOLOGY},
Volume = {139},
Number = {2},
Pages = {427-427},
Year = {1982},
ISSN = {0361-803X},
url = {http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:A1982NY43600075&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=47d3190e77e5a3a53558812f597b0b92},
Key = {Johnson82f}
}
@article{fds132799,
Author = {ML Banson and GP Cofer and R Black and GA Johnson},
Title = {A probe for specimen magnetic resonance microscopy.},
Journal = {Investigative radiology, UNITED STATES},
Volume = {27},
Number = {2},
Pages = {157-64},
Year = {1992},
Month = {February},
ISSN = {0020-9996},
Keywords = {Copper • Equipment Design • Evaluation Studies
• Magnetic Resonance Spectroscopy • Microscopy
• Polytetrafluoroethylene • Sensitivity and
Specificity • instrumentation •
methods*},
Abstract = {One of the primary limits to spatial resolution in magnetic
resonance (MR) microscopy is the limited signal. The purpose
of this study is to build a radiofrequency (rf) probe for MR
microscopy of fixed specimens at 300 MHz. The design
criteria for the probe were (1) high sensitivity; (2) good
rf homogeneity; (3) minimization of BO variations. All
probes were Helmholtz pairs operating at 300 MHz. Coils were
constructed from copper/Teflon/copper microwave substrate
which eliminated susceptibility problems from solder and
discrete capacitors. Signal-to-noise ratio (SNR) was
compared with a conventional solenoid. Phantoms of agar gels
and saline-filled tubes were used to characterize the SNR
and homogeneity. SNR measurements of the coil pairs showed a
marked improvement (up to 60%) over that of the reference
solenoid. The region of homogeneity was defined as a 10%
variation in signal intensity. This correlated with the
coil's inner diameter. Graphs of SNR versus diameter,
separation, and copper foil width allowed for optimization
of the structure. Using this coil, MR microscopy is now
possible on small, fixed specimens with pixels as small as
20 x 20 x 30 microns. Work is currently under way exploiting
the SNR and homogeneity provided by this probe to determine
the degree to which MR microscopy might add to the
pathologists' diagnostic tools.},
Key = {fds132799}
}
@booklet{Banson92a,
Author = {Banson, ML and Cofer, GP and Black, R and Johnson,
GA},
Title = {A probe for specimen magnetic resonance microscopy.},
Journal = {Invest Radiol},
Volume = {27},
Number = {2},
Pages = {157-164},
Year = {1992},
Month = {February},
ISSN = {0020-9996},
url = {http://www.ncbi.nlm.nih.gov/pubmed/1601608},
Abstract = {One of the primary limits to spatial resolution in magnetic
resonance (MR) microscopy is the limited signal. The purpose
of this study is to build a radiofrequency (rf) probe for MR
microscopy of fixed specimens at 300 MHz. The design
criteria for the probe were (1) high sensitivity; (2) good
rf homogeneity; (3) minimization of BO variations. All
probes were Helmholtz pairs operating at 300 MHz. Coils were
constructed from copper/Teflon/copper microwave substrate
which eliminated susceptibility problems from solder and
discrete capacitors. Signal-to-noise ratio (SNR) was
compared with a conventional solenoid. Phantoms of agar gels
and saline-filled tubes were used to characterize the SNR
and homogeneity. SNR measurements of the coil pairs showed a
marked improvement (up to 60%) over that of the reference
solenoid. The region of homogeneity was defined as a 10%
variation in signal intensity. This correlated with the
coil's inner diameter. Graphs of SNR versus diameter,
separation, and copper foil width allowed for optimization
of the structure. Using this coil, MR microscopy is now
possible on small, fixed specimens with pixels as small as
20 x 20 x 30 microns. Work is currently under way exploiting
the SNR and homogeneity provided by this probe to determine
the degree to which MR microscopy might add to the
pathologists' diagnostic tools.},
Doi = {10.1097/00004424-199202000-00013},
Key = {Banson92a}
}
@article{fds268757,
Author = {Calabrese, E and Badea, A and Watson, C and Johnson,
GA},
Title = {A quantitative magnetic resonance histology atlas of
postnatal rat brain development with regional estimates of
growth and variability.},
Journal = {Neuroimage},
Volume = {71},
Pages = {196-206},
Year = {2013},
Month = {May},
url = {http://www.ncbi.nlm.nih.gov/pubmed/23353030},
Abstract = {There has been growing interest in the role of postnatal
brain development in the etiology of several neurologic
diseases. The rat has long been recognized as a powerful
model system for studying neuropathology and the safety of
pharmacologic treatments. However, the complex
spatiotemporal changes that occur during rat
neurodevelopment remain to be elucidated. This work
establishes the first magnetic resonance histology (MRH)
atlas of the developing rat brain, with an emphasis on
quantitation. The atlas comprises five specimens at each of
nine time points, imaged with eight distinct MR contrasts
and segmented into 26 developmentally defined brain regions.
The atlas was used to establish a timeline of morphometric
changes and variability throughout neurodevelopment and
represents a quantitative database of rat neurodevelopment
for characterizing rat models of human neurologic
disease.},
Doi = {10.1016/j.neuroimage.2013.01.017},
Key = {fds268757}
}
@booklet{Solomon03,
Author = {D. Solomon and M. Schiffman and R. Tarone and E. E.
Partridge and L. Kilgore and S. Hester and J. L. Walker and G. A. Johnson and A. Yadack and R. S. Guido and K.
Mcintyre-seltman and R. P. Edwards and J. Gruss and N. B.
Kiviat and L. Koutsky and C. Mao a},
Title = {A randomized trial on the management of low-grade squamous
intraepithelial lesion cytology interpretations},
Journal = {American Journal Of Obstetrics And Gynecology},
Volume = {188},
Number = {6},
Pages = {1393 -- 1400},
Year = {2003},
Month = {June},
Key = {Solomon03}
}
@article{fds373572,
Author = {Tian, Y and Johnson, GA and Williams, RW and White,
LE},
Title = {A rapid workflow for neuron counting in combined light sheet
microscopy and magnetic resonance histology.},
Journal = {Front Neurosci},
Volume = {17},
Pages = {1223226},
Year = {2023},
url = {http://dx.doi.org/10.3389/fnins.2023.1223226},
Abstract = {Information on regional variation in cell numbers and
densities in the CNS provides critical insight into
structure, function, and the progression of CNS diseases.
However, variability can be real or a consequence of methods
that do not account for technical biases, including
morphologic deformations, errors in the application of cell
type labels and boundaries of regions, errors of counting
rules and sampling sites. We address these issues in a mouse
model by introducing a workflow that consists of the
following steps: 1. Magnetic resonance histology (MRH) to
establish the size, shape, and regional morphology of the
mouse brain in situ. 2. Light-sheet microscopy (LSM) to
selectively label neurons or other cells in the entire brain
without sectioning artifacts. 3. Register LSM volumes to MRH
volumes to correct for dissection errors and both global and
regional deformations. 4. Implement stereological protocols
for automated sampling and counting of cells in 3D LSM
volumes. This workflow can analyze the cell densities of one
brain region in less than 1 min and is highly replicable in
cortical and subcortical gray matter regions and structures
throughout the brain. This method demonstrates the advantage
of not requiring an extensive amount of training data,
achieving a F1 score of approximately 0.9 with just 20
training nuclei. We report deformation-corrected neuron
(NeuN) counts and neuronal density in 13 representative
regions in 5 C57BL/6J cases and 2 BXD strains. The data
represent the variability among specimens for the same brain
region and across regions within the specimen. Neuronal
densities estimated with our workflow are within the range
of values in previous classical stereological studies. We
demonstrate the application of our workflow to a mouse model
of aging. This workflow improves the accuracy of neuron
counting and the assessment of neuronal density on a
region-by-region basis, with broad applications for studies
of how genetics, environment, and development across the
lifespan impact cell numbers in the CNS.},
Doi = {10.3389/fnins.2023.1223226},
Key = {fds373572}
}
@booklet{Bazer99,
Author = {F. W. Bazer and G. A. Johnson and A. G. Stagg and K. M.
Taylor and E. Gootwine and A. Gertler and T. E.
Spencer},
Title = {A servomechanism in the ovine uterus regulates endometrial
gene expression.},
Journal = {Biology Of Reproduction},
Volume = {60},
Pages = {150 -- 150},
Year = {1999},
Key = {Bazer99}
}
@booklet{Johnson95a,
Author = {G. A. Johnson and D. A. Mortensen and A. R.
Martin},
Title = {A simulation of herbicide use based on weed
spatial-distribution},
Journal = {Weed Research},
Volume = {35},
Number = {3},
Pages = {197 -- 205},
Year = {1995},
Month = {June},
Key = {Johnson95a}
}
@booklet{Johnson96d,
Author = {G. A. Johnson and G. A. Livesay and S. L. Y. Woo and K. R.
Rajagopal},
Title = {A single integral finite strain viscoelastic model of
ligaments and tendons},
Journal = {Journal Of Biomechanical Engineering-transactions Of The
Asme},
Volume = {118},
Number = {2},
Pages = {221 -- 226},
Year = {1996},
Month = {May},
Key = {Johnson96d}
}
@article{fds174112,
Author = {GA Johnson and GA Livesay and SL Woo and KR Rajagopal},
Title = {A single integral finite strain viscoelastic model of
ligaments and tendons.},
Journal = {Journal of biomechanical engineering},
Volume = {118},
Number = {2},
Pages = {221-6},
Year = {1996},
Month = {May},
ISSN = {0148-0731},
Keywords = {Adult • Aged • Aged, 80 and over • Animals
• Dogs • Elasticity • Humans • Ligaments
• Middle Aged • Models, Biological* •
Nonlinear Dynamics • Patella • Stress, Mechanical
• Tendons • Viscosity • physiology •
physiology*},
Abstract = {A general continuum model for the nonlinear viscoelastic
behavior of soft biological tissues was formulated. This
single integral finite strain (SIFS) model describes finite
deformation of a nonlinearly viscoelastic material within
the context of a three-dimensional model. The specific form
describing uniaxial extension was obtained, and the idea of
conversion from one material to another (at a microscopic
level) was then introduced to model the nonlinear behavior
of ligaments and tendons. Conversion allowed different
constitutive equations to be used for describing a single
ligament or tendon at different strain levels. The model was
applied to data from uniaxial extension of younger and older
human patellar tendons and canine medial collateral
ligaments. Model parameters were determined from
curve-fitting stress-strain and stress-relaxation data and
used to predict the time-dependent stress generated by
cyclic extensions.},
Language = {eng},
Key = {fds174112}
}
@booklet{Johnson00c,
Author = {G. A. Johnson and S. V. Mantha and T. A.
Day},
Title = {A spectrofluorometric survey of UV-induced blue-green
fluorescence in foliage of 35 species},
Journal = {Journal Of Plant Physiology},
Volume = {156},
Number = {2},
Pages = {242 -- 252},
Year = {2000},
Month = {February},
Key = {Johnson00c}
}
@article{fds132853,
Author = {GA Johnson and CE Ravin},
Title = {A survey of digital chest radiography.},
Journal = {Radiologic clinics of North America, UNITED
STATES},
Volume = {21},
Number = {4},
Pages = {655-64},
Year = {1983},
Month = {December},
ISSN = {0033-8389},
Keywords = {Humans • Methods • Radiographic Image Enhancement
• Radiography, Thoracic* • Subtraction Technique*
• Technology, Radiologic • Tomography, X-Ray
Computed*},
Abstract = {The problems of chest radiography as they relate to digital
systems are described, the current approaches to these
problems are reviewed, and the utility of digital chest
radiography is demonstrated.},
Key = {fds132853}
}
@booklet{Johnson83a,
Author = {Johnson, GA and Ravin, CE},
Title = {A survey of digital chest radiography.},
Journal = {Radiol Clin North Am},
Volume = {21},
Number = {4},
Pages = {655-664},
Year = {1983},
Month = {December},
ISSN = {0033-8389},
url = {http://www.ncbi.nlm.nih.gov/pubmed/6361846},
Abstract = {The problems of chest radiography as they relate to digital
systems are described, the current approaches to these
problems are reviewed, and the utility of digital chest
radiography is demonstrated.},
Key = {Johnson83a}
}
@article{fds268817,
Author = {Bowden, DM and Johnson, GA and Zaborsky, L and Green, WDK and Moore, E and Badea, A and Dubach, MF and Bookstein, FL},
Title = {A symmetrical Waxholm canonical mouse brain for
NeuroMaps.},
Journal = {J Neurosci Methods},
Volume = {195},
Number = {2},
Pages = {170-175},
Year = {2011},
Month = {February},
ISSN = {1872-678X},
url = {http://www.ncbi.nlm.nih.gov/pubmed/21163300},
Keywords = {Animals • Brain • Brain Mapping* • Functional
Laterality • Image Processing, Computer-Assisted •
Magnetic Resonance Imaging • Mice • Mice, Inbred
C57BL • Models, Neurological • anatomy &
histology* • methods • methods*},
Abstract = {NeuroMaps (2010) is a Web-based application that enables
investigators to map data from macaque studies to a
canonical atlas of the macaque brain. It currently serves as
an image processor enabling them to create figures suitable
for publication, presentation and archival purposes.
Eventually it will enable investigators studying any of
several species to analyze the overlap between their data
and multimodality data mapped by others. The purpose of the
current project was to incorporate the Waxholm canonical
mouse brain (Harwylycz, 2009) into NeuroMaps. An enhanced
gradient echo (T2*) magnetic resonance image (MRI) of the
Waxholm canonical brain (Johnson et al., 2010) was warped to
bring the irregular biological midplane of the MRI into line
with the mathematically flat midsagittal plane of the
Waxholm space. The left hemisphere was deleted and the right
hemisphere reflected to produce a symmetrical 3D MR image.
The symmetrical T2* image was imported into NeuroMaps. The
map executing this warp was applied to four other voxellated
volumes based on the same canonical specimen and maintained
at the Center for In-Vitro Microscopy (CIVM): a T2-weighted
MRI, a T1-weighted MRI, a segmented image and an image
reconstructed from Nissl-stained histological sections of
the specimen. Symmetric versions of those images were
returned to the CIVM repository where they are made
available to other laboratories. Utility of the symmetric
atlas was demonstrated by mapping and comparing a number of
cortical areas as illustrated in three conventional mouse
brain atlases. The symmetric Waxholm mouse brain atlas is
now accessible in NeuroMaps where investigators can map
image data to standard templates over the Web and process
them for publication, presentation and archival purposes:
http://braininfo.rprc.washington.edu/MapViewData.aspx.},
Language = {eng},
Doi = {10.1016/j.jneumeth.2010.11.028},
Key = {fds268817}
}
@booklet{Johnson83,
Author = {Johnson, GA and Suddarth, S and Dunnick, NR},
Title = {A system for digital videodensitometry},
Journal = {Proceedings of SPIE - The International Society for Optical
Engineering},
Volume = {419},
Pages = {222-227},
Year = {1983},
Month = {December},
url = {http://dx.doi.org/10.1117/12.936029},
Doi = {10.1117/12.936029},
Key = {Johnson83}
}
@booklet{Johnson83b,
Author = {JOHNSON, GA and DANIELEY, N and SUDDARTH, S and DUNNICK,
NR},
Title = {A SYSTEM FOR DIGITAL VIDEODENSITOMETRY},
Journal = {AMERICAN JOURNAL OF ROENTGENOLOGY},
Volume = {141},
Number = {6},
Pages = {1360-1361},
Year = {1983},
ISSN = {0361-803X},
url = {http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:A1983RT41800084&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=47d3190e77e5a3a53558812f597b0b92},
Key = {Johnson83b}
}
@article{fds359544,
Author = {Xiao, J and Hornburg, KJ and Cofer, G and Cook, JJ and Pratson, F and Qi,
Y and Johnson, GA},
Title = {A time-course study of actively stained mouse brains:
Diffusion tensor imaging parameters and connectomic
stability over 1 year.},
Journal = {NMR Biomed},
Volume = {35},
Number = {1},
Pages = {e4611},
Year = {2022},
Month = {January},
url = {http://dx.doi.org/10.1002/nbm.4611},
Abstract = {While the application of diffusion tensor imaging (DTI),
tractography, and connectomics to fixed tissue is a common
practice today, there have been limited studies examining
the effects of fixation on brain microstructure over
extended periods. This mouse model time-course study reports
the changes of regional brain volumes and diffusion scalar
parameters, such as fractional anisotropy, across 12
representative brain regions as measures of brain structural
stability. The scalar DTI parameters and regional volumes
were highly variable over the first 2 weeks after fixation.
The same parameters were consistent over a 2-8-week window
after fixation, which means confounds from tissue stability
over that scanning window were minimal. Quantitative
connectomes were analyzed over the same time with extension
out to 1 year. While there was some change in the scalar
metrics at 1 year after fixation, these changes were
sufficiently small, particularly in white matter, to support
reproducible connectomes over a period ranging from 2-weeks
to 1-year post-fixation. These findings delineate a scanning
period, during which brain volumes, diffusion scalar
metrics, and connectomes are remarkably consistent.},
Doi = {10.1002/nbm.4611},
Key = {fds359544}
}
@article{fds132740,
Author = {G.A. Johnson},
Title = {A. LeFurgey, S. Gewalt, N. Wallace, D. Kopf, G.A. Johnson,
P. Ingram. Magnetic Resonance Microscoopy of the Adult
Barnacle. in "Proc., MicroCosmopolitan (14th Australian
Conference on Electron Microscopy, 1st Meeting,
International Union of Microbeam Analysis Societies, 9th
Symposium of the Microscopical Society of Australia).
Syndey, Australia, 1996," p. 105.},
Year = {1996},
Key = {fds132740}
}
@booklet{Griffin98,
Author = {W. P. Griffin and E. B. Savage and R. E. Clark and J. J.
Pacella and G. A. Johnson and J. A. Magovern and G. J.
Magovern},
Title = {AB-180 circulatory support system - Summary of development
and phase I clinical trial},
Journal = {Asaio Journal},
Volume = {44},
Number = {5},
Pages = {M719 -- M724},
Year = {1998},
Key = {Griffin98}
}
@article{fds174075,
Author = {WP Griffin and EB Savage and RE Clark and JJ Pacella and GA Johnson and JA
Magovern, GJ Magovern Sr},
Title = {AB-180 circulatory support system: summary of development
and phase I clinical trial.},
Journal = {ASAIO journal (American Society for Artificial Internal
Organs : 1992)},
Volume = {44},
Number = {5},
Pages = {M719-24},
Year = {1999},
Month = {February},
ISSN = {1058-2916},
Keywords = {Feasibility Studies • Heart-Assist Devices* •
Humans • Male • Middle Aged • Prosthesis
Design},
Abstract = {The AB-180 Circulatory Support System (AB-180 CSS; Cardiac
Assist Technologies, Pittsburgh, PA) is a left ventricular
assist system for investigational use in patients with
postcardiotomy cardiogenic shock who are refractory to
standard treatment with an intra-aortic balloon pump,
pharmacologic treatment, or both. The intent of the AB-180
CSS is to provide temporary (up to 14 days) mechanical
circulatory support until the heart recovers adequate
mechanical function. The system consists of a small
implantable centrifugal pump and a controller. A unique
infusion system produces a hydrodynamic bearing between
rotational and stationary components of the AB-180 CSS pump.
This infusion system also provides a source of heparin for
localized anticoagulation. Extensive bench and animal work
has illustrated anticoagulation requirements, established
operating guidelines, and demonstrated safety and efficacy.
An investigational device exemption has been granted for a
Phase I, five patient feasibility study at Allegheny General
Hospital in Pittsburgh, Pennsylvania. To date, the pump has
been implanted in one patient. The results from this first
case are presented here.},
Language = {eng},
Key = {fds174075}
}
@article{fds310029,
Author = {Oliverio, MI and Delnomdedieu, M and Best, CF and Li, P and Morris, M and Callahan, MF and Johnson, GA and Smithies, O and Coffman,
TM},
Title = {Abnormal water metabolism in mice lacking the type 1A
receptor for ANG II.},
Journal = {Am J Physiol Renal Physiol},
Volume = {278},
Number = {1},
Pages = {F75-F82},
Year = {2000},
Month = {January},
ISSN = {1931-857X},
url = {http://www.ncbi.nlm.nih.gov/pubmed/10644657},
Keywords = {Animals • Body Weight • Deamino Arginine
Vasopressin • Female • Genotype • Kidney
• Kidney Concentrating Ability • Losartan •
Male • Mice • Osmolar Concentration •
Receptor, Angiotensin, Type 1 • Receptors, Angiotensin
• Urine • Urodynamics • Vasopressins •
Water • Water Deprivation • anatomy & histology
• antagonists & inhibitors • blood •
chemistry • deficiency* • drug effects •
genetics • metabolism* • pharmacology •
physiology*},
Abstract = {Mice lacking AT(1A) receptors for ANG II have a defect in
urinary concentration manifested by an inability to increase
urinary osmolality to levels seen in controls after
thirsting. This defect results in extreme serum
hypertonicity during water deprivation. In the basal state,
plasma vasopressin levels are similar in wild-type controls
and Agtr1a -/- mice. Plasma vasopressin levels increase
normally in the AT(1A) receptor-deficient mice after 24 h of
water deprivation, suggesting that the defect in urine
concentration is intrinsic to the kidney. Using magnetic
resonance microscopy, we find that the absence of AT(1A)
receptors is associated with a modest reduction in the
distance from the kidney surface to the tip of the papilla.
However, this structural abnormality seems to play little
role in the urinary concentrating defect in Agtr1a -/- mice
since the impairment is largely reproduced in wild-type mice
by treatment with an AT(1)-receptor antagonist. These
studies demonstrate a critical role for the AT(1A) receptor
in maintaining inner medullary structures in the kidney and
in regulating renal water excretion.},
Doi = {10.1152/ajprenal.2000.278.1.F75},
Key = {fds310029}
}
@article{fds341986,
Author = {Wang, N and Cofer, G and Anderson, RJ and Qi, Y and Liu, C and Johnson,
GA},
Title = {Accelerating quantitative susceptibility imaging acquisition
using compressed sensing.},
Journal = {Phys Med Biol},
Volume = {63},
Number = {24},
Pages = {245002},
Year = {2018},
Month = {December},
url = {http://dx.doi.org/10.1088/1361-6560/aaf15d},
Abstract = {To evaluate the feasibility of compressed sensing (CS) for
accelerating quantitative susceptibility mapping (QSM)
acquisition in MR histology, control, demyelination, and
remyelination mice were scanned using a modified 3D gradient
echo (GRE) pulse sequence (allowing undersampling the
k-space in two phase dimensions) at 9.4 T. Fully sampled and
CS data at various acceleration factors (AF) (4.0-16.0) were
acquired with high spatial resolution of 22.5 µm3
isotropic. For prospective scans, the local phase was
reconstructed slice-by-slice using a nonlinear
reconstruction algorithm with phase cycling, and the QSM was
calculated using the iLSQR method. Susceptibility values of
major white matter tracts at various CS AF were compared
with the fully sampled data as ground truth. The
reconstructed QSM preserved major information with
negligible artifacts, even at CS AF of 8.0 and 16.0. The
maximum variation of susceptibility value of control mice
was 5.8% in the fimbria (FI) area with the acceleration
factor of 16.0, compared to the ground truth. The maximum
variation was 9.5% in corpus callosum (CC) area of the
demyelination mouse brain with the acceleration factor of
8.0. The maximum variation was 8.2% in AC area of
remyelination mouse brain with 16.0 acceleration factor.
This preliminary study shows that CS can reduce the
acquisition time of GRE MRI in MR histology without losing
significant accuracy in quantitative susceptibility
values.},
Doi = {10.1088/1361-6560/aaf15d},
Key = {fds341986}
}
@booklet{Scribner00,
Author = {D. R. Scribner and J. Baldwin and G. A. Johnson},
Title = {Actinomycosis mimicking a pelvic malignancy - A case
report},
Journal = {Journal Of Reproductive Medicine},
Volume = {45},
Number = {6},
Pages = {515 -- 518},
Year = {2000},
Month = {June},
Key = {Scribner00}
}
@article{fds174257,
Author = {DR Scribner Jr and J Baldwin and GA Johnson},
Title = {Actinomycosis mimicking a pelvic malignancy. A case
report.},
Journal = {The Journal of reproductive medicine},
Volume = {45},
Number = {6},
Pages = {515-8},
Year = {2000},
Month = {June},
ISSN = {0024-7758},
Keywords = {Abdominal Pain • Actinomycosis • Colorectal
Neoplasms • Diagnosis, Differential • Female
• Humans • Middle Aged • Uterine Diseases
• complications • diagnosis • diagnosis*
• etiology* • pathology •
surgery},
Abstract = {BACKGROUND: Pelvic actinomycosis is difficult to diagnose
preoperatively. The chronic infection is locally
infiltrative and causes a profound induration of infected
tissue planes. This induration, combined with absence of
fever and leukocytosis, can mimic a pelvic malignancy. CASE:
A 55-year-old woman was diagnosed with a pelvic mass after a
two-month history of intermittent lower abdominal pain. The
patient had had an intrauterine device for 12 years; it was
removed two months prior to an exploratory laparotomy for
the symptomatic mass. The mass was highly suggestive of
colorectal cancer, with the rectosigmoid colon indurated and
adherent to the uterus and sacrum. The induration of the
colon extended caudally to within 3 cm of the anal verge. An
abdominoperineal resection was performed along with a total
abdominal hysterectomy, bilateral salpingo-oophorectomy and
colostomy. Pathology revealed acute and chronic
endometritis, left tuboovarian abscess and extensive, acute
inflammation of the rectosigmoid colon without evidence of
diverticuli. Actinomycosis was diagnosed based on the
characteristic sulphur granules seen on hemotoxylin and
eosin staining. CONCLUSION: Actinomycosis can mimic pelvic
and abdominal malignancies. Surgeons should be aware of this
infection to potentially spare women morbidity from
excessive surgical procedures.},
Language = {eng},
Key = {fds174257}
}
@booklet{Jaeger00,
Author = {L. A. Jaeger and R. C. Burghardt and G. A. Johnson and F. W.
Bazer},
Title = {Activation of conceptus and maternal integrins by
transforming growth factor beta latency associated
peptide.},
Journal = {Biology Of Reproduction},
Volume = {62},
Pages = {281 -- 281},
Year = {2000},
Key = {Jaeger00}
}
@article{fds268838,
Author = {Petiet, A and Johnson, GA},
Title = {Active staining of mouse embryos for magnetic resonance
microscopy.},
Journal = {Methods Mol Biol},
Volume = {611},
Pages = {141-149},
Year = {2010},
ISSN = {1940-6029},
url = {http://www.ncbi.nlm.nih.gov/pubmed/19960328},
Keywords = {Animals • Brain • Embryo, Mammalian •
Formaldehyde • Magnetic Resonance Imaging • Mice
• Microscopy • Staining and Labeling • Tissue
Fixation • chemistry • metabolism •
metabolism* • methods • methods*},
Abstract = {Magnetic resonance histology (MRH) has found considerable
application in structural phenotyping in the mouse embryo.
MRH employs the same fundamental principles as clinical MRI,
albeit with spatial resolution up to six orders of magnitude
higher than that in clinical studies. Critical to obtaining
this enormous gain in resolution is the need to enhance the
weak signal from these microscopic voxels. This has been
accomplished through the use of active staining, a method to
simultaneously fix the embryonic/fetal tissues, while
reducing the spin lattice relaxation time (T1). We describe
here the methods that allow one to balance the fixation,
which reduces the nuclear magnetic resonance (NMR) signal,
with the enhancement of signal derived from the reduction in
T1. Methods are included to cover the ranges of embryonic
specimens from E10.5 through E19.5.},
Language = {eng},
Doi = {10.1007/978-1-60327-345-9_11},
Key = {fds268838}
}
@article{fds268699,
Author = {Papp, EA and Leergaard, TB and Calabrese, E and Johnson, GA and Bjaalie,
JG},
Title = {Addendum to “Waxholm Space atlas of the Sprague Dawley rat
brain” [NeuroImage 97 (2014) 374-386].},
Journal = {Neuroimage},
Volume = {105},
Pages = {561-562},
Year = {2015},
Month = {January},
ISSN = {1053-8119},
url = {http://dx.doi.org/10.1016/j.neuroimage.2014.10.017},
Abstract = {The main focus of our original article was to describe the
anatomical delineations constituting the first version of
the WHS Sprague Dawley atlas, apply the Waxholm Space
coordinate system, and publish the associated MRI/DTI
template and segmentation volume in their original format.
To increase usability of the dataset, we have recently
shared an updated version of the volumetric image material
(v1.01). The aims of this addendum are to inform about the
improvements in the updated dataset, in particular related
to navigation in the WHS coordinate system, and provide
guidance for transforming coordinates acquired in the first
version of the atlas.},
Doi = {10.1016/j.neuroimage.2014.10.017},
Key = {fds268699}
}
@booklet{Miyoshi89,
Author = {K. Miyoshi and J. J. Pouch and S. A. Alterovitz and D. M.
Pantic and G. A. Johnson},
Title = {Adhesion, friction, and wear of plasma-deposited thin
silicon-nitride films at temperatures to
700-degrees-c},
Journal = {Wear},
Volume = {133},
Number = {1},
Pages = {107 -- 123},
Year = {1989},
Month = {September},
Key = {Miyoshi89}
}
@article{fds311651,
Author = {Vetreno, RP and Yaxley, R and Paniagua, B and Johnson, GA and Crews,
FT},
Title = {Adult rat cortical thickness changes across age and
following adolescent intermittent ethanol
treatment.},
Journal = {Addict Biol},
Volume = {22},
Number = {3},
Pages = {712-723},
Year = {2017},
Month = {May},
ISSN = {1355-6215},
url = {http://dx.doi.org/10.1111/adb.12364},
Abstract = {Human studies have established that adolescence is a period
of brain maturation that parallels the development of adult
behaviors. However, little is known regarding cortical
development in the adult rat brain. We used magnetic
resonance imaging (MRI) and histology to assess the impact
of age on adult Wistar rat cortical thickness on postnatal
day (P)80 and P220 as well as the effect of adolescent binge
ethanol exposure on adult (P80) cortical thickness. MRI
revealed changes in cortical thickness between P80 and P220
that differ across cortical region. The adult P220 rat
prefrontal cortex increased in thickness whereas cortical
thinning occurred in both the cingulate and parietal
cortices relative to young adult P80 rats. Histological
analysis confirmed the age-related cortical thinning. In the
second series of experiments, an animal model of adolescent
intermittent ethanol (AIE; 5.0 g/kg, intragastrically, 20
percent ethanol w/v, 2 days on/2 days off from P25 to
P55) was used to assess the effects of alcohol on cortical
thickness in young adult (P80) rats. MRI revealed that AIE
resulted in region-specific cortical changes. A small region
within the prefrontal cortex was significantly thinner
whereas medial cortical regions were significantly thicker
in young adult (P80) AIE-treated rats. The observed increase
in cortical thickness was confirmed by histology. Thus, the
rat cerebral cortex continues to undergo cortical thickness
changes into adulthood, and adolescent alcohol exposure
alters the young adult cortex that could contribute to brain
dysfunction in adulthood.},
Doi = {10.1111/adb.12364},
Key = {fds311651}
}
@article{fds174230,
Author = {GA Johnson and VR Gutti and SK Loyalka and KA O'Beirne 2nd and SK
Cochran, HM Dale and GR Kracke},
Title = {Albuterol metered dose inhaler performance under hyperbaric
pressures.},
Journal = {Undersea & hyperbaric medicine : journal of the Undersea and
Hyperbaric Medical Society, Inc},
Volume = {36},
Number = {1},
Pages = {55-63},
Year = {2009},
Month = {April},
ISSN = {1066-2936},
Keywords = {Aerosols • Albuterol • Analysis of Variance •
Asthma • Bronchial Spasm • Bronchodilator Agents
• Diving • Humans • Hyperbaric Oxygenation
• Metered Dose Inhalers • Nanoparticles •
Particle Size • Pressure* • Weights and Measures
• administration & dosage* • adverse effects
• chemistry • contraindications • etiology
• physiology • physiopathology • standards*
• therapy},
Abstract = {The weight change per actuation and aerosol particle size
and number delivered by albuterol metered dose inhalers
(MDIs) were measured in a multiplace hyperbaric chamber at
pressures ranging from one atmosphere absolute (1 ATA, 0
feet of seawater, fsw, 101 kPa) to three ATA (66 fsw, 304
kPa). Weight change per actuation by CFC
(chlorofluorocarbon) and long canister HFA
(hydrofluoroalkane) powered MDIs was 13 +/- 1% and 12 +/- 1%
less, respectively, at 3 ATA compared to 1 ATA. However,
weight change per actuation by short canister HFA MDIs was
not significantly changed with pressure. The geometric mean
diameters of nano particles from the CFC and short canister
HFA MDIs decreased from 50 nm at 0 fsw to 32 nm at 66 fsw
whereas the long canister HFA aerosol diameters were not
affected. The numbers of nanometer size particles delivered
at 66 fsw were only 4-7% of those delivered at 0 fsw for the
CFC and long canister HFA MDIs whereas for the short
canister MDIs it was 26%. We conclude that the weight change
per actuation of albuterol and the sizes and numbers of
aerosol particles emitted from albuterol MDIs actuated in a
hyperbaric environment vary by canister type.},
Language = {eng},
Key = {fds174230}
}
@booklet{Macfall94a,
Author = {MacFall, JS and Spaine, P and Doudrick, R and Johnson,
GA},
Title = {Alterations in growth and water-transport processes in
fusiform rust galls of pine, determined by magnetic
resonance microscopy},
Journal = {Phytopathology},
Volume = {84},
Number = {3},
Pages = {288-293},
Year = {1994},
Month = {January},
url = {http://dx.doi.org/10.1094/Phyto-84-288},
Doi = {10.1094/Phyto-84-288},
Key = {Macfall94a}
}
@article{fds343278,
Author = {Antonsen, BT and Jiang, Y and Veraart, J and Qu, H and Nguyen, HP and Sijbers, J and Hörsten, SV and Johnson, GA and Leergaard,
TB},
Title = {Altered diffusion tensor imaging measurements in aged
transgenic Huntington disease rats},
Journal = {Brain Structure and Function},
Volume = {218},
Number = {3},
Pages = {767-778},
Year = {2013},
url = {http://dx.doi.org/10.1007/s00429-012-0427-0},
Abstract = {Rodent models of Huntington disease (HD) are valuable tools
for investigating HD pathophysiology and evaluating new
therapeutic approaches. Non-invasive characterization of
HD-related phenotype changes is important for monitoring
progression of pathological processes and possible effects
of interventions. The first transgenic rat model for HD
exhibits progressive late-onset affective, cognitive, and
motor impairments, as well as neuropathological features
reflecting observations from HD patients. In this report, we
contribute to the anatomical phenotyping of this model by
comparing high-resolution ex vivo DTI measurements obtained
in aged transgenic HD rats and wild-type controls. By region
of interest analysis supplemented by voxel-based statistics,
we find little evidence of atrophy in basal ganglia regions,
but demonstrate altered DTI measurements in the dorsal and
ventral striatum, globus pallidus, entopeduncular nucleus,
substantia nigra, and hippocampus. These changes are largely
compatible with DTI findings in preclinical and clinical HD
patients. We confirm earlier reports that HD rats express a
moderate neuropathological phenotype, and provide evidence
of altered DTI measures in specific HD-related brain
regions, in the absence of pronounced morphometric changes.
© 2012 The Author(s).},
Doi = {10.1007/s00429-012-0427-0},
Key = {fds343278}
}
@article{fds268771,
Author = {Antonsen, BT and Jiang, Y and Veraart, J and Qu, H and Nguyen, HP and Sijbers, J and von Hörsten, S and Johnson, GA and Leergaard,
TB},
Title = {Altered diffusion tensor imaging measurements in aged
transgenic Huntington disease rats.},
Journal = {Brain Struct Funct},
Volume = {218},
Number = {3},
Pages = {767-778},
Year = {2013},
Month = {May},
ISSN = {1863-2653},
url = {http://dx.doi.org/10.1007/s00429-012-0427-0},
Abstract = {Rodent models of Huntington disease (HD) are valuable tools
for investigating HD pathophysiology and evaluating new
therapeutic approaches. Non-invasive characterization of
HD-related phenotype changes is important for monitoring
progression of pathological processes and possible effects
of interventions. The first transgenic rat model for HD
exhibits progressive late-onset affective, cognitive, and
motor impairments, as well as neuropathological features
reflecting observations from HD patients. In this report, we
contribute to the anatomical phenotyping of this model by
comparing high-resolution ex vivo DTI measurements obtained
in aged transgenic HD rats and wild-type controls. By region
of interest analysis supplemented by voxel-based statistics,
we find little evidence of atrophy in basal ganglia regions,
but demonstrate altered DTI measurements in the dorsal and
ventral striatum, globus pallidus, entopeduncular nucleus,
substantia nigra, and hippocampus. These changes are largely
compatible with DTI findings in preclinical and clinical HD
patients. We confirm earlier reports that HD rats express a
moderate neuropathological phenotype, and provide evidence
of altered DTI measures in specific HD-related brain
regions, in the absence of pronounced morphometric
changes.},
Doi = {10.1007/s00429-012-0427-0},
Key = {fds268771}
}
@article{fds174312,
Author = {X Li and FW Bazer and H Gao and W Jobgen and GA Johnson and P Li, JR
McKnight and MC Satterfield and TE Spencer and G Wu},
Title = {Amino acids and gaseous signaling.},
Journal = {Amino acids},
Volume = {37},
Number = {1},
Pages = {65-78},
Year = {2009},
Month = {May},
ISSN = {1438-2199},
url = {http://dx.doi.org/10.1007/s00726-009-0264-5},
Keywords = {Amino Acids • Animals • Carbon Monoxide •
Cardiovascular Diseases • Energy Metabolism •
Humans • Hydrogen Sulfide • Immune System Diseases
• Nervous System Diseases • Nitric Oxide •
Signal Transduction • Sulfur Dioxide •
biosynthesis* • metabolism • metabolism* •
physiology},
Abstract = {Gases, such as nitric oxide (NO), carbon monoxide (CO),
hydrogen sulfide (H(2)S), and sulfur dioxide (SO(2)) are
known toxic pollutants in the air. However, they are now
recognized as important signaling molecules synthesized in
animals and humans from arginine, glycine (heme), and
cysteine, respectively. At physiological levels, NO, CO, and
SO(2) activate guanylyl cyclase to generate cGMP which
elicits a variety of responses (including relaxation of
vascular smooth muscle cells, hemodynamics,
neurotransmission, and cell metabolism) via cGMP-dependent
protein kinases. H(2)S is also a crucial regulator of both
neurological function and endothelium-dependent relaxation
through cGMP-independent mechanisms involving stimulation of
membrane K(ATP) channels and intracellular cAMP signaling.
Additionally, NO, CO, and H(2)S confer cytoprotective and
immunomodulatory effects. Moreover, NH(3) is a major product
of amino acid catabolism and profoundly affects the function
of neurons and the vasculature through glutamine-dependent
inhibition of NO synthesis. Emerging evidence shows that
amino acids are not only precursors for these endogenous
gases, but are also regulators of their production in a
cell-specific manner. Thus, recent advances on gaseous
signaling have greatly expanded our basic knowledge of amino
acid biochemistry and nutrition. These exciting discoveries
will aid in the design of new nutritional and
pharmacological means to prevent and treat major health
problems related to developmental biology and nutrient
metabolism, including intrauterine growth restriction,
preterm birth, aging, neurological disorders, cancer,
obesity, diabetes, and cardiovascular disease.},
Language = {eng},
Doi = {10.1007/s00726-009-0264-5},
Key = {fds174312}
}
@article{fds268720,
Author = {Subashi, E and Choudhury, KR and Johnson, GA},
Title = {An analysis of the uncertainty and bias in DCE-MRI
measurements using the spoiled gradient-recalled echo pulse
sequence.},
Journal = {Med Phys},
Volume = {41},
Number = {3},
Pages = {032301},
Year = {2014},
Month = {March},
ISSN = {0094-2405},
url = {http://dx.doi.org/10.1118/1.4865790},
Abstract = {PURPOSE: The pharmacokinetic parameters derived from dynamic
contrast-enhanced (DCE) MRI have been used in more than 100
phase I trials and investigator led studies. A comparison of
the absolute values of these quantities requires an
estimation of their respective probability distribution
function (PDF). The statistical variation of the DCE-MRI
measurement is analyzed by considering the fundamental
sources of error in the MR signal intensity acquired with
the spoiled gradient-echo (SPGR) pulse sequence. METHODS:
The variance in the SPGR signal intensity arises from
quadrature detection and excitation flip angle
inconsistency. The noise power was measured in 11 phantoms
of contrast agent concentration in the range [0-1] mM (in
steps of 0.1 mM) and in onein vivo acquisition of a
tumor-bearing mouse. The distribution of the flip angle was
determined in a uniform 10 mM CuSO4 phantom using the spin
echo double angle method. The PDF of a wide range of T1
values measured with the varying flip angle (VFA) technique
was estimated through numerical simulations of the SPGR
equation. The resultant uncertainty in contrast agent
concentration was incorporated in the most common model of
tracer exchange kinetics and the PDF of the derived
pharmacokinetic parameters was studied numerically. RESULTS:
The VFA method is an unbiased technique for measuringT1 only
in the absence of bias in excitation flip angle. The
time-dependent concentration of the contrast agent measured
in vivo is within the theoretically predicted uncertainty.
The uncertainty in measuring K(trans) with SPGR pulse
sequences is of the same order, but always higher than, the
uncertainty in measuring the pre-injection longitudinal
relaxation time (T10). The lowest achievable
bias/uncertainty in estimating this parameter is
approximately 20%-70% higher than the bias/uncertainty in
the measurement of the pre-injection T1 map. The fractional
volume parameters derived from the extended Tofts model were
found to be extremely sensitive to the variance in signal
intensity. The SNR of the pre-injection T1 map indicates the
limiting precision with which K(trans) can be calculated.
CONCLUSIONS: Current small-animal imaging systems and pulse
sequences robust to motion artifacts have the capacity for
reproducible quantitative acquisitions with DCE-MRI. In
these circumstances, it is feasible to achieve a level of
precision limited only by physiologic variability.},
Doi = {10.1118/1.4865790},
Key = {fds268720}
}
@article{fds174264,
Author = {GA Johnson and E Harrington},
Title = {An apparatus for the simultaneous demineralization of
fifty-four specimens.},
Journal = {Stain technology},
Volume = {52},
Number = {3},
Pages = {127-9},
Year = {1977},
Month = {May},
ISSN = {0038-9153},
Keywords = {Evaluation Studies as Topic • Formic Acids •
Histological Techniques • Specimen Handling •
instrumentation*},
Abstract = {An apparatus designed to demineralize 54 specimens
simultaneously is described. A drum with built-in specimen
holders rotates continuously through a bath of acid,
allowing a free exchange of demineralizing fluid over the
specimens. Individual specimens can be easily introduced or
withdrawn from the apparatus without disturbing
others.},
Language = {eng},
Key = {fds174264}
}
@article{fds174195,
Author = {GA JOHNSON},
Title = {An arsine problem: engineering notes.},
Journal = {American Industrial Hygiene Association quarterly},
Volume = {14},
Number = {3},
Pages = {188-90},
Year = {1953},
Month = {September},
ISSN = {0096-820X},
Language = {eng},
Key = {fds174195}
}
@article{fds268752,
Author = {Johnson, GA and Cofer, GP and Gewalt, SL and Hedlund,
LW},
Title = {An engineering approach to image-based phenotyping},
Journal = {2002 IEEE INTERNATIONAL SYMPOSIUM ON BIOMEDICAL IMAGING,
PROCEEDINGS},
Pages = {381-383},
Publisher = {IEEE},
Year = {2002},
ISBN = {0-7803-7584-X},
url = {http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000178000400095&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=47d3190e77e5a3a53558812f597b0b92},
Doi = {10.1109/isbi.2002.1029273},
Key = {fds268752}
}
@booklet{Halvorsen81,
Author = {Halvorsen, RA and Woodfield, S and T., R and Allen, SM and Hedlung, LW and Johnson, GA and Thompson, WM},
Title = {AN EVALUATION OF CONTRAST AGENTS FOR CT OF THE HEPATOBILIARY
SYSTEM},
Journal = {Investigative Radiology},
Volume = {16},
Number = {5},
Pages = {387-387},
Publisher = {Ovid Technologies (Wolters Kluwer Health)},
Year = {1981},
Month = {September},
ISSN = {0020-9996},
url = {http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:A1981MK56200053&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=47d3190e77e5a3a53558812f597b0b92},
Doi = {10.1097/00004424-198109000-00053},
Key = {Halvorsen81}
}
@booklet{Johnson81,
Author = {JOHNSON, GA and KOROBKIN, M and HEINZ, R},
Title = {AN EVALUATION OF MULTIPLANAR IMAGING CAPABILITIES OF 4
CURRENT CT SCANNERS},
Journal = {AMERICAN JOURNAL OF ROENTGENOLOGY},
Volume = {136},
Number = {6},
Pages = {1279-1279},
Year = {1981},
ISSN = {0361-803X},
url = {http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:A1981LU08900097&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=47d3190e77e5a3a53558812f597b0b92},
Key = {Johnson81}
}
@article{fds268891,
Author = {Johnson, GA and O'Foghludha, F},
Title = {An experimental "trans-molybdenum" tube for
mammography.},
Journal = {Radiology},
Volume = {127},
Number = {2},
Pages = {511-516},
Year = {1978},
Month = {May},
ISSN = {0033-8419},
url = {http://www.ncbi.nlm.nih.gov/pubmed/644079},
Keywords = {Fluorescence • Humans • Intubation •
Mammography • Molybdenum • Radiation Dosage •
instrumentation*},
Abstract = {Possible mammographic advantages of "trans-molybdenum"
anodes (atomic number greater than ZMo) are decreased dose
because the fluorescent radiation is more penetrating, and
increased useful output; contrast degradation is known to be
tolerable. The output per mAs, the HVT in Al, and the
penetration in Lucite were measured spectroscopically for an
experimental Rh-anode tube and also for Mo- and W-anode
mammographic tubes. The trans-molybdenum tube was shown to
have output and dose advantages over Mo anodes, and output
and contrast advantages over normal and selectively filtered
W anodes. Possible applications in areas other than
mammography are briefly discussed.},
Doi = {10.1148/127.2.511},
Key = {fds268891}
}
@booklet{Neiswanger87,
Author = {L. Neiswanger and G. A. Johnson and V. P.
Carey},
Title = {An experimental-study of high rayleigh number mixed
convection in a rectangular enclosure with restricted inlet
and outlet openings},
Journal = {Journal Of Heat Transfer-transactions Of The
Asme},
Volume = {109},
Number = {2},
Pages = {446 -- 453},
Year = {1987},
Month = {May},
Key = {Neiswanger87}
}
@article{fds341989,
Author = {Anderson, RJ and Cook, JJ and Delpratt, NA and Nouls, JC and Gu, B and McNamara, JO and Avants, BB and Johnson, GA and Badea,
A},
Title = {An HPC Pipeline with Validation Framework for Small Animal
Multivariate Brain Analysis (SAMBA).},
Journal = {CoRR},
Volume = {abs/1709.10483},
Year = {2017},
Key = {fds341989}
}
@booklet{Johnson80d,
Author = {JOHNSON, GA and FOGHLUDA, F},
Title = {AN INVESTIGATION OF TRANSMOLYBDENUM FLUORESCENT ANODES FOR
MAMMOGRAPHY},
Journal = {AMERICAN JOURNAL OF ROENTGENOLOGY},
Volume = {134},
Number = {4},
Pages = {855-855},
Year = {1980},
ISSN = {0361-803X},
url = {http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:A1980JM11100062&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=47d3190e77e5a3a53558812f597b0b92},
Key = {Johnson80d}
}
@book{fds292752,
Author = {Paxinos, G and Watson, C and Calabrese, E and Badea, A and Johnson,
G},
Title = {An MRI/DTI Atlas of the Rat Brain},
Pages = {224 pages},
Publisher = {Elsevier, Academic Press},
Year = {2015},
Month = {May},
ISBN = {978-0-12-417313-2},
Abstract = {MRI/DTI Atlas of the Rat Brain offers two major enhancements
when compared with earlier attempts to make MRI/DTI rat
brain atlases. First, the spatial resolution at 25μm is
considerably higher than previous data published. Secondly,
the comprehensive set of MRI/DTI contrasts provided has
enabled the authors to identify more than 80% of structures
identified in The Rat Brain in Stereotaxic
Coordinates.},
Key = {fds292752}
}
@article{fds268756,
Author = {Calabrese, E and Johnson, GA and Watson, C},
Title = {An ontology-based segmentation scheme for tracking postnatal
changes in the developing rodent brain with
MRI.},
Journal = {Neuroimage},
Volume = {67},
Pages = {375-384},
Year = {2013},
Month = {February},
url = {http://www.ncbi.nlm.nih.gov/pubmed/23246176},
Abstract = {The postnatal period of neurodevelopment has been implicated
in a number of brain disorders including autism and
schizophrenia. Rodent models have proven to be invaluable in
advancing our understanding of the human brain, and will
almost certainly play a pivotal role in future studies on
postnatal neurodevelopment. The growing field of magnetic
resonance microscopy has the potential to revolutionize our
understanding of neurodevelopment, if it can be successfully
and appropriately assimilated into the vast body of existing
neuroscience research. In this study, we demonstrate the
utility of a developmental neuro-ontology designed
specifically for tracking regional changes in MR biomarkers
throughout postnatal neurodevelopment. Using this
ontological classification as a segmentation guide, we track
regional changes in brain volume in rats between postnatal
day zero and postnatal day 80 and demonstrate differential
growth rates in axial versus paraxial brain regions. Both
the ontology and the associated label volumes are provided
as a foundation for future MR-based studies of postnatal
neurodevelopment in normal and disease states.},
Doi = {10.1016/j.neuroimage.2012.11.037},
Key = {fds268756}
}
@booklet{Pals86,
Author = {D. T. Pals and S. Thaisrivongs and J. A. Lawson and W. M.
Kati and S. R. Turner and G. L. Degraaf and D. W. Harris and G. A. Johnson},
Title = {An orally active inhibitor of renin},
Journal = {Hypertension},
Volume = {8},
Number = {12},
Pages = {1105 -- 1112},
Year = {1986},
Month = {December},
Key = {Pals86}
}
@article{fds174084,
Author = {DT Pals and S Thaisrivongs and JA Lawson and WM Kati, SR Turner and GL
DeGraaf, DW Harris and GA Johnson},
Title = {An orally active inhibitor of renin.},
Journal = {Hypertension},
Volume = {8},
Number = {12},
Pages = {1105-12},
Year = {1986},
Month = {December},
ISSN = {0194-911X},
Keywords = {Administration, Oral • Animals • Blood Pressure
• Dose-Response Relationship, Drug • Heart Rate
• Humans • Macaca fascicularis • Male •
Oligopeptides • Rats • Rats, Inbred Strains •
Renin • Renin-Angiotensin System • Sodium •
antagonists & inhibitors* • drug effects •
pharmacology* • physiology},
Abstract = {A potent renin inhibitor, U-71038 (Boc-Pro-Phe-N-MeHis-Leu
psi[CHOHCH2]Val-Ile-Amp), was tested for oral effectiveness.
Enzyme kinetic studies indicated that U-71038 was a
competitive inhibitor of hog renin with an inhibitor
constant (Ki) value of 12 nM. Intravenous as well as oral
administration of U-71038 to anesthetized, ganglion-blocked
rats infused with hog renin elicited dose-related
hypotensive responses. Intravenous administration of U-71038
to conscious, sodium-depleted monkeys caused dose-related
decreases of blood pressure and plasma renin activity
without affecting heart rate. Similarly, the oral
administration of U-71038 at 50 mg/kg to conscious,
sodium-depleted monkeys elicited a pronounced hypotension
and decrease in plasma renin activity that persisted for 5
hours. The hypotensive responses elicited by intravenous and
oral administration of U-71038 to hog renin-infused rats and
sodium-depleted monkeys were shown to be due entirely to
inhibition of the renin-angiotensin system. A comparison of
the results obtained after the intravenous administration of
U-71038 with the results obtained after the oral
administration of U-71038 implied that at least 10% of the
orally administered U-71038 must have been absorbed to cause
the observed effects in hog renin-infused rats and
sodium-depleted monkeys. The studies demonstrated that an
inhibitor of renin with a long duration of action and with
oral effectiveness is a feasible entity.},
Language = {eng},
Key = {fds174084}
}
@booklet{Pals86a,
Author = {D. T. Pals and S. Thaisrivongs and J. A. Lawson and W. M.
Kati and S. R. Turner and G. L. Degraaf and D. W. Harris and G. A. Johnson},
Title = {An orally-active inhibitor of renin},
Journal = {Hypertension},
Volume = {8},
Number = {9},
Pages = {833 -- 833},
Year = {1986},
Month = {September},
Key = {Pals86a}
}
@booklet{Stafford80,
Author = {M. L. Stafford and K. F. Guin and G. A. Johnson and L. A.
Sanders and S. L. Rockey},
Title = {Analysis of 1,4-dioxane in ethoxylated surfactants},
Journal = {Journal Of The Society Of Cosmetic Chemists},
Volume = {31},
Number = {6},
Pages = {281 -- 287},
Year = {1980},
Key = {Stafford80}
}
@booklet{Garlow02,
Author = {J. E. Garlow and H. Ka and G. A. Johnson and R. C. Burghardt and L. A. Jaeger and F. W. Bazer},
Title = {Analysis of osteopontin at the maternal-placental interface
in pigs},
Journal = {Biology Of Reproduction},
Volume = {66},
Number = {3},
Pages = {718 -- 725},
Year = {2002},
Month = {March},
Key = {Garlow02}
}
@article{fds174252,
Author = {JE Garlow and H Ka and GA Johnson and RC Burghardt and LA Jaeger and FW
Bazer},
Title = {Analysis of osteopontin at the maternal-placental interface
in pigs.},
Journal = {Biology of reproduction},
Volume = {66},
Number = {3},
Pages = {718-25},
Year = {2002},
Month = {March},
ISSN = {0006-3363},
Keywords = {Animals • Cell Membrane • Endometrium •
Epithelial Cells • Female • Frozen Sections •
In Situ Hybridization • Integrins • Oligopeptides
• Osteopontin • Placenta • Pregnancy •
RNA, Messenger • Sialoglycoproteins • Signal
Transduction • Swine* • Trophoblasts • Uterus
• analysis • analysis* • chemistry •
chemistry* • genetics • metabolism •
pharmacology • physiology},
Abstract = {Noninvasive, epitheliochorial placentation in the pig
follows a prolonged preimplantation period characterized by
migration, spacing and elongation of conceptuses, and
secretion of estrogen for maternal recognition of pregnancy.
Osteopontin (OPN) is an extracellular matrix protein that
binds integrins to promote cell-cell attachment and
communication. OPN appears to play a key role in conceptus
implantation and maintenance of pregnancy in sheep; however,
a role for OPN in the porcine uterus has not been
established. Therefore, this study examined OPN expression
and function in the porcine uterus and conceptus
(embryo/fetus and associated extraembryonic membranes).
Northern and slot blot hybridization detected an increase in
endometrial OPN expression between Days 25 and 30, and
levels remained elevated through Day 85 of pregnancy. In
situ hybridization localized OPN mRNA to discrete regions of
the uterine luminal epithelium (LE) on Day 15 of pregnancy
and to the entire LE thereafter. Glandular epithelial (GE)
expression of OPN mRNA was first detected on Day 35 of
pregnancy and increased through Day 85. Both 70- and 45-kDa
forms of OPN protein were detected in cyclic and pregnant
endometrium by Western blotting. OPN protein was localized
to the LE and GE by immunofluorescence; however, only the
70-kDa OPN was detected in uterine flushings. OPN protein
was present along the entire uterine-placental interface
after Day 30 of pregnancy. In addition, OPN mRNA and protein
were localized to immune-like cells within the stratum
compactum of the endometrium in both Day 9 cyclic and
pregnant gilts. Incubation of OPN-coated microbeads with
porcine trophectoderm and uterine luminal epithelial cells
induced Arg-Gly-Asp (RGD)-dependent integrin activation and
transmembrane accumulation of cytoskeletal molecules at the
apical cell surface as assessed by immunofluorescence
detection of talin or alpha-actinin as markers for focal
adhesions. These results suggest that OPN, expressed by
uterine epithelium and immune cells, may interact with
receptors (i.e., integrins) on conceptus and uterus to
promote conceptus development and signaling between these
tissues as key contributors to attachment and placentation
in the pig.},
Language = {eng},
Key = {fds174252}
}
@article{fds268721,
Author = {Ashton, JR and Befera, N and Clark, D and Qi, Y and Mao, L and Rockman, HA and Johnson, GA and Badea, CT},
Title = {Anatomical and functional imaging of myocardial infarction
in mice using micro-CT and eXIA 160 contrast
agent.},
Journal = {Contrast Media Mol Imaging},
Volume = {9},
Number = {2},
Pages = {161-168},
Year = {2014},
ISSN = {1555-4309},
url = {http://dx.doi.org/10.1002/cmmi.1557},
Abstract = {Noninvasive small animal imaging techniques are essential
for evaluation of cardiac disease and potential
therapeutics. A novel preclinical iodinated contrast agent
called eXIA 160 has recently been developed, which has been
evaluated for micro-CT cardiac imaging. eXIA 160 creates
strong contrast between blood and tissue immediately after
its injection and is subsequently taken up by the myocardium
and other metabolically active tissues over time. We focus
on these properties of eXIA and show its use in imaging
myocardial infarction in mice. Five C57BL/6 mice were imaged
~2 weeks after left anterior descending coronary artery
ligation. Six C57BL/6 mice were used as controls.
Immediately after injection of eXIA 160, an enhancement
difference between blood and myocardium of ~340 HU enabled
cardiac function estimation via 4D micro-CT scanning with
retrospective gating. Four hours post-injection, the healthy
perfused myocardium had a contrast difference of ~140 HU
relative to blood while the infarcted myocardium showed no
enhancement. These differences allowed quantification of
infarct size via dual-energy micro-CT. In vivo micro-SPECT
imaging and ex vivo triphenyl tetrazolium chloride (TTC)
staining provided validation for the micro-CT findings. Root
mean squared error of infarct measurements was 2.7% between
micro-CT and SPECT, and 4.7% between micro-CT and TTC. Thus,
micro-CT with eXIA 160 can be used to provide both
morphological and functional data for preclinical studies
evaluating myocardial infarction and potential therapies.
Further studies are warranted to study the potential use of
eXIA 160 as a CT molecular imaging tool for other
metabolically active tissues in the mouse.},
Doi = {10.1002/cmmi.1557},
Key = {fds268721}
}
@booklet{Bristow81,
Author = {M. R. Bristow and W. A. Minobe and M. E. Billingham and J.
B. Marmor and G. A. Johnson and B. M. Ishimoto and W. S.
Sageman and J. R. Daniels},
Title = {Anthracycline-associated cardiac and renal damage in rabbits
- evidence for mediation by vasoactive substances},
Journal = {Laboratory Investigation},
Volume = {45},
Number = {2},
Pages = {157 -- 168},
Year = {1981},
Key = {Bristow81}
}
@article{fds174100,
Author = {MR Bristow and WA Minobe and ME Billingham and JB Marmor and GA Johnson and BM Ishimoto and WS Sageman, JR Daniels},
Title = {Anthracycline-associated cardiac and renal damage in
rabbits. Evidence for mediation by vasoactive
substances.},
Journal = {Laboratory investigation; a journal of technical methods and
pathology},
Volume = {45},
Number = {2},
Pages = {157-68},
Year = {1981},
Month = {August},
ISSN = {0023-6837},
Keywords = {Animals • Arteries • Cardiomyopathies •
Catecholamines • Doxorubicin • Female •
Histamine Antagonists • Histamine Release • Kidney
Diseases • Myocardium • Rabbits •
Vasoconstrictor Agents • Vasodilator Agents •
adverse effects* • blood • chemically induced*
• metabolism • pharmacology •
pharmacology*},
Abstract = {We tested the hypothesis that anthracycline-induced cardiac
and renal damage is mediated by vasoactive substances. A
1-minute exposure to 5 micrograms per ml. of doxorubicin
(DXR, Adriamycin) produced cardiac histamine release in
isolated rabbit hearts. Under conditions in which histamine
uptake and metabolism were impaired, the administration of
DXR, 2 mg. per kg., over 1 minute was associated with
elevations in arterial histamine and catecholamines. The
chronic weekly administration of DXR produced severe cardiac
and renal damage. The administration of combined histaminic
and adrenergic blockade with diphenhydramine, cimetidine,
phentolamine, and propranolol (DCPP) pre- and immediately
post-DXR resulted in near total protection against
DXR-mediated cardiac damage and prevented the majority of
the renal lesions. The combined administration of
diphenhydramine, cimetidine, phentolamine, and propranolol
did not appear to be acting by mechanisms other than
blockade of vasoactive amine receptors as cardiac uptake of
DXR and the DXR antitumor response were not altered by
diphenhydramine, cimetidine, phentolamine, and propranolol.
This study demonstrates that anthracycline-associated
cardiac and renal toxicity may be mediated by vasoactive
substances and that anthracycline cardiomyopathy is
potentially preventable.},
Language = {eng},
Key = {fds174100}
}
@booklet{Burvill95a,
Author = {P. W. Burvill and G. A. Johnson and K. D. Jamrozik and C. S.
Anderson and E. G. Stewartwynne and T. M. H.
Chakera},
Title = {Anxiety disorders after stroke - results from the perth
community stroke study},
Journal = {British Journal Of Psychiatry},
Volume = {166},
Pages = {328 -- 332},
Year = {1995},
Month = {March},
Key = {Burvill95a}
}
@article{fds174259,
Author = {PW Burvill and GA Johnson and KD Jamrozik and CS Anderson and EG
Stewart-Wynne, TM Chakera},
Title = {Anxiety disorders after stroke: results from the Perth
Community Stroke Study.},
Journal = {The British journal of psychiatry : the journal of mental
science},
Volume = {166},
Number = {3},
Pages = {328-32},
Year = {1995},
Month = {March},
ISSN = {0007-1250},
Keywords = {Adaptation, Psychological • Adult • Aged •
Agoraphobia • Anxiety Disorders • Cerebrovascular
Disorders • Comorbidity • Cross-Sectional Studies
• Delirium, Dementia, Amnestic, Cognitive Disorders
• Depressive Disorder • Female • Follow-Up
Studies • Humans • Incidence • Male •
Middle Aged • Sick Role • Western Australia •
diagnosis • epidemiology • epidemiology* •
psychology},
Abstract = {BACKGROUND: The prevalence of anxiety disorders in 294
patients who survived to four months in the Perth Community
Stroke Study (Perth, Australia), and a follow-up of these
patients at 12 months, are presented. METHOD: Diagnoses are
described both in the usual DSM hierarchic format and by a
non-hierarchic approach. Adoption of the hierarchic approach
alone greatly underestimates the prevalence of anxiety
disorders. RESULTS: Most cases were of agoraphobia, and the
remainder were generalised anxiety disorder. The prevalence
of anxiety disorders alone was 5% in men and 19% in women;
in community controls, it was 5% in men and 8% in women.
Adopting a non-hierarchic approach to diagnosis gave a
prevalence of 12% in men and 28% in women. When those who
showed evidence of anxiety disorder before stroke were
subtracted, the latter prevalence was 9% in men and 20% in
women. CONCLUSION: One-third of the men and half of the
women with post-stroke anxiety disorders showed evidence of
either depression or an anxiety disorder at the time of the
stroke. At 12 month follow-up of 49 patients with
agoraphobia by a non-hierarchic approach, 51% had recovered,
and equal proportions of the remainder had died or still had
agoraphobia. The only major difference in outcome between
those with anxiety disorder alone and those with comorbid
depression was the greater mortality in the
latter.},
Language = {eng},
Key = {fds174259}
}
@booklet{Burg02,
Author = {Burg, KJL and Delnomdedieu, M and Beiler, RJ and Culberson, CR and Greene, KG and Halberstadt, CR and Holder, WD and Loebsack, AB and Roland, WD and Johnson, GA},
Title = {Application of magnetic resonance microscopy to tissue
engineering: a polylactide model.},
Journal = {J Biomed Mater Res},
Volume = {61},
Number = {3},
Pages = {380-390},
Year = {2002},
Month = {September},
ISSN = {0021-9304},
url = {http://www.ncbi.nlm.nih.gov/pubmed/12115463},
Abstract = {Absorbable polymers are unique materials that find
application as temporary scaffolds in tissue engineering.
They are often extremely sensitive to histological
processing and, for this reason, studying fragile,
tissue-engineered constructs before implantation can be
quite difficult. This research investigates the use of
noninvasive imaging using magnetic resonance microscopy
(MRM) as a tool to enhance the assessment of these cellular
constructs. A series of cellular, polylactide constructs was
developed and analyzed using a battery of tests, including
MRM. Distribution of rat aortic smooth muscle cells within
the scaffolds was compared as one example of a tissue
engineering MRM application. Cells were loaded in varying
amounts using static and dynamic methods. It was found that
the cellular component was readily identified and the
polymer microstructure readily assessed. Specifically, the
MRM results showed a heterogeneous distribution of cells due
to static loading and a homogenous distribution associated
with dynamic loading, results that were not visible through
biochemical tests, scanning electron microscopy, or
histological evaluation independently. MRM also allowed
differentiation between different levels of cellular
loading. The current state of MRM is such that it is
extremely useful in the refinement of polymer processing and
cell seeding methods. This method has the potential, with
technological advances, to be of future use in the
characterization of cell-polymer interactions.},
Doi = {10.1002/jbm.10146},
Key = {Burg02}
}
@article{fds132755,
Author = {KJ Burg and M Delnomdedieu and RJ Beiler and CR Culberson and KG Greene and CR Halberstadt and WD Holder and AB Loebsack and WD Roland and GA
Johnson},
Title = {Application of magnetic resonance microscopy to tissue
engineering: a polylactide model.},
Journal = {Journal of biomedical materials research, United
States},
Volume = {61},
Number = {3},
Pages = {380-90},
Year = {2002},
Month = {September},
ISSN = {0021-9304},
Keywords = {Absorbable Implants • Animals • Aorta •
Biocompatible Materials • Cell Survival • Magnetic
Resonance Imaging* • Materials Testing •
Microscopy • Muscle, Smooth, Vascular • Polyesters
• Porosity • Rats • Tissue Engineering •
cytology • instrumentation • instrumentation*
• methods • methods*},
Abstract = {Absorbable polymers are unique materials that find
application as temporary scaffolds in tissue engineering.
They are often extremely sensitive to histological
processing and, for this reason, studying fragile,
tissue-engineered constructs before implantation can be
quite difficult. This research investigates the use of
noninvasive imaging using magnetic resonance microscopy
(MRM) as a tool to enhance the assessment of these cellular
constructs. A series of cellular, polylactide constructs was
developed and analyzed using a battery of tests, including
MRM. Distribution of rat aortic smooth muscle cells within
the scaffolds was compared as one example of a tissue
engineering MRM application. Cells were loaded in varying
amounts using static and dynamic methods. It was found that
the cellular component was readily identified and the
polymer microstructure readily assessed. Specifically, the
MRM results showed a heterogeneous distribution of cells due
to static loading and a homogenous distribution associated
with dynamic loading, results that were not visible through
biochemical tests, scanning electron microscopy, or
histological evaluation independently. MRM also allowed
differentiation between different levels of cellular
loading. The current state of MRM is such that it is
extremely useful in the refinement of polymer processing and
cell seeding methods. This method has the potential, with
technological advances, to be of future use in the
characterization of cell-polymer interactions.},
Key = {fds132755}
}
@article{fds268871,
Author = {De Lin and M and Toncheva, G and Nguyen, G and Kim, S and Anderson-Evans,
C and Johnson, GA and Yoshizumi, TT},
Title = {Application of MOSFET detectors for dosimetry in small
animal radiography using short exposure times.},
Journal = {Radiat Res},
Volume = {170},
Number = {2},
Pages = {260-263},
Year = {2008},
Month = {August},
ISSN = {0033-7587},
url = {http://www.ncbi.nlm.nih.gov/pubmed/18666818},
Keywords = {Equipment Design • Equipment Failure Analysis •
Radiation Dosage • Radiographic Image Enhancement
• Radiometry • Sensitivity and Specificity •
Transducers* • Transistors* • instrumentation*
• methods},
Abstract = {Digital subtraction angiography (DSA) X-ray imaging for
small animals can be used for functional phenotyping given
its ability to capture rapid physiological changes at high
spatial and temporal resolution. The higher temporal and
spatial requirements for small-animal imaging drive the need
for short, high-flux X-ray pulses. However, high doses of
ionizing radiation can affect the physiology. The purpose of
this study was to verify and apply metal oxide semiconductor
field effect transistor (MOSFET) technology to dosimetry for
small-animal diagnostic imaging. A tungsten anode X-ray
source was used to expose a tissue-equivalent mouse phantom.
Dose measurements were made on the phantom surface and
interior. The MOSFETs were verified with thermoluminescence
dosimeters (TLDs). Bland-Altman analysis showed that the
MOSFET results agreed with the TLD results (bias, 0.0625).
Using typical small animal DSA scan parameters, the dose
ranged from 0.7 to 2.2 cGy. Application of the MOSFETs in
the small animal environment provided two main benefits: (1)
the availability of results in near real-time instead of the
hours needed for TLD processes and (2) the ability to
support multiple exposures with different X-ray techniques
(various of kVp, mA and ms) using the same MOSFET. This
MOSFET technology has proven to be a fast, reliable small
animal dosimetry method for DSA imaging and is a good system
for dose monitoring for serial and gene expression
studies.},
Doi = {10.1667/RR1328.1},
Key = {fds268871}
}
@article{fds268881,
Author = {Maronpot, RR and Sills, RC and Johnson, GA},
Title = {Applications of magnetic resonance microscopy.},
Journal = {Toxicol Pathol},
Volume = {32 Suppl 2},
Pages = {42-48},
Year = {2004},
ISSN = {0192-6233},
url = {http://www.ncbi.nlm.nih.gov/pubmed/15503663},
Keywords = {Animals • Brain • Fetus • Humans • Image
Processing, Computer-Assisted • Imaging,
Three-Dimensional • Magnetic Resonance Imaging* •
Microscopy • Sulfur Oxides • Teratology •
Urate Oxidase • genetics • metabolism •
methods*},
Abstract = {Magnetic resonance imaging (MRI) has enjoyed enormous
clinical success since the first demonstration of the method
more than 30 years ago. An increasing number of
pharmaceutical manufacturers seeking new biomarkers for
assessing drug efficacy and toxicity are turning to MRI. A
specific application of MRI promises to revolutionize
pathology for the basic scientist in the same way MRI has
forever altered the standard of care in the clinical domain.
More specifically, this application is the use of magnetic
resonance microscopy (MRM) in conjunction with new staining
methodologies that now make MRM routinely available to the
widest range of investigators.},
Doi = {10.1080/01926230490451707},
Key = {fds268881}
}
@article{fds174168,
Author = {RS Dhariwal and RK Fitch and CL Lavelle and GA Johnson},
Title = {Artefacts observed on dental tissues during ion etching in a
scanning electron microscope.},
Journal = {Journal of anatomy},
Volume = {122},
Number = {Pt 1},
Pages = {133-40},
Year = {1976},
Month = {September},
ISSN = {0021-8782},
Keywords = {Dental Amalgam • Dental Enamel • Dental Materials
• Dentin • Histological Techniques • Humans
• Ions • Microscopy, Electron, Scanning •
Tooth • methods* • ultrastructure •
ultrastructure*},
Language = {eng},
Key = {fds174168}
}
@booklet{Anderson93,
Author = {C. S. Anderson and K. D. Jamrozik and P. W. Burvill and T.
M. H. Chakera and G. A. Johnson and E. G.
Stewartwynne},
Title = {Ascertaining the true incidence of stroke - experience from
the perth community stroke study, 1989-1990},
Journal = {Medical Journal Of Australia},
Volume = {158},
Number = {2},
Pages = {80 -- 84},
Year = {1993},
Month = {January},
Key = {Anderson93}
}
@article{fds174290,
Author = {CS Anderson and KD Jamrozik and PW Burvill and TM Chakera and GA
Johnson, EG Stewart-Wynne},
Title = {Ascertaining the true incidence of stroke: experience from
the Perth Community Stroke Study, 1989-1990.},
Journal = {The Medical journal of Australia},
Volume = {158},
Number = {2},
Pages = {80-4},
Year = {1993},
Month = {January},
ISSN = {0025-729X},
Keywords = {Adolescent • Adult • Age Factors • Aged
• Aged, 80 and over • Cerebrovascular Disorders
• Confidence Intervals • Female • Follow-Up
Studies • Humans • Incidence • Ischemic
Attack, Transient • Male • Middle Aged • Sex
Factors • Western Australia • epidemiology •
epidemiology* • mortality},
Abstract = {OBJECTIVE: To determine the age and sex specific incidence,
and case fatality of stroke in Perth, Western Australia.
DESIGN AND SETTING: A population-based descriptive
epidemiological study. SUBJECTS: All residents of a
geographically defined segment of the Perth metropolitan
area (population 138,708) who had a stroke or transient
ischaemic attack between 20 February 1989 and 19 August
1990, inclusive. MAIN OUTCOME MEASURES: Definite acute
"first-ever-in-a-lifetime" (first-ever) and recurrent stroke
classified according to standard definitions and criteria.
RESULTS: During the 18-month study period, 536 stroke events
occurred among 492 patients, 69% of which were first-ever
strokes. The crude annual event rate for all strokes was 258
(95% confidence interval 231-285) per 100,000, and the
overall case fatality at 28 days was 24% (95% CI, 20%-28%).
The crude annual incidence for first-ever strokes was 178
(95% CI, 156-200) per 100,000; 189 (95% CI, 157-221) per
100,000 in males and 166 (95% CI, 136-196) per 100,000 in
females. The corresponding rates, age-adjusted to the
"world" population, were 132 (95% CI, 109-155) for males and
77 (95% CI, 60-94) for females. CONCLUSIONS: In contrast to
mortality rates for ischaemic heart disease, the incidence
of stroke in Australia appears little different from that
for several other Western countries. For both males and
females the incidence of stroke rises exponentially with
increasing age. Although the sex-dependent difference in the
risk of stroke is greatest in middle age, males are at
greater risk of stroke even among the most elderly. To
determine the incidence of stroke accurately,
population-based studies of stroke need exhaustive and
overlapping sources of case ascertainment. If only cases
admitted to hospital had been used, we would have
underestimated the rate of stroke among the most elderly by
almost 40%. We estimate that approximately 37,000 people,
about 50% of whom are over the age of 75, suffer a stroke
each year in Australia.},
Language = {eng},
Key = {fds174290}
}
@article{fds268719,
Author = {Lee, C-L and Min, H and Befera, N and Clark, D and Qi, Y and Das, S and Johnson, GA and Badea, CT and Kirsch, DG},
Title = {Assessing cardiac injury in mice with dual energy-microCT,
4D-microCT, and microSPECT imaging after partial heart
irradiation.},
Journal = {Int J Radiat Oncol Biol Phys},
Volume = {88},
Number = {3},
Pages = {686-693},
Year = {2014},
Month = {March},
ISSN = {0360-3016},
url = {http://dx.doi.org/10.1016/j.ijrobp.2013.11.238},
Abstract = {PURPOSE: To develop a mouse model of cardiac injury after
partial heart irradiation (PHI) and to test whether dual
energy (DE)-microCT and 4-dimensional (4D)-microCT can be
used to assess cardiac injury after PHI to complement
myocardial perfusion imaging using micro-single photon
emission computed tomography (SPECT). METHODS AND MATERIALS:
To study cardiac injury from tangent field irradiation in
mice, we used a small-field biological irradiator to deliver
a single dose of 12 Gy x-rays to approximately one-third of
the left ventricle (LV) of Tie2Cre; p53(FL/+) and Tie2Cre;
p53(FL/-) mice, where 1 or both alleles of p53 are deleted
in endothelial cells. Four and 8 weeks after irradiation,
mice were injected with gold and iodinated
nanoparticle-based contrast agents, and imaged with
DE-microCT and 4D-microCT to evaluate myocardial vascular
permeability and cardiac function, respectively.
Additionally, the same mice were imaged with microSPECT to
assess myocardial perfusion. RESULTS: After PHI with tangent
fields, DE-microCT scans showed a time-dependent increase in
accumulation of gold nanoparticles (AuNp) in the myocardium
of Tie2Cre; p53(FL/-) mice. In Tie2Cre; p53(FL/-) mice,
extravasation of AuNp was observed within the irradiated LV,
whereas in the myocardium of Tie2Cre; p53(FL/+) mice, AuNp
were restricted to blood vessels. In addition, data from
DE-microCT and microSPECT showed a linear correlation (R(2)
= 0.97) between the fraction of the LV that accumulated AuNp
and the fraction of LV with a perfusion defect. Furthermore,
4D-microCT scans demonstrated that PHI caused a markedly
decreased ejection fraction, and higher end-diastolic and
end-systolic volumes, to develop in Tie2Cre; p53(FL/-) mice,
which were associated with compensatory cardiac hypertrophy
of the heart that was not irradiated. CONCLUSIONS: Our
results show that DE-microCT and 4D-microCT with
nanoparticle-based contrast agents are novel imaging
approaches complementary to microSPECT for noninvasive
assessment of the change in myocardial vascular permeability
and cardiac function of mice in whom myocardial injury
develops after PHI.},
Doi = {10.1016/j.ijrobp.2013.11.238},
Key = {fds268719}
}
@article{fds132756,
Author = {HH Qiu and GP Cofer and LW Hedlund and GA Johnson},
Title = {Automated feedback control of body temperature for small
animal studies with MR microscopy.},
Journal = {IEEE transactions on bio-medical engineering, UNITED
STATES},
Volume = {44},
Number = {11},
Pages = {1107-13},
Year = {1997},
Month = {November},
ISSN = {0018-9294},
Keywords = {Animals • Body Temperature • Equipment Design
• Feedback • Ferrets • Guinea Pigs •
Magnetic Resonance Imaging • Mice • Microscopy
• Models, Biological • Monitoring, Physiologic
• Rats • Respiration, Artificial • Signal
Processing, Computer-Assisted* • Thermometers* •
instrumentation • methods • physiology*},
Abstract = {A temperature control system consisting of a thermistor,
signal processor, and computer algorithm was developed for
magnetic resonance (MR) microscopy of small live animals.
With control of body temperature within +/- 0.2 degree C of
the set point, heart rate is stabilized and, in turn,
repetition time (TR) during cardiac-gated studies is less
variable. Thus, image quality and resolution are
improved.},
Key = {fds132756}
}
@booklet{Qiu97,
Author = {Qiu, HH and Cofer, GP and Hedlund, LW and Johnson,
GA},
Title = {Automated feedback control of body temperature for small
animal studies with MR microscopy.},
Journal = {IEEE Trans Biomed Eng},
Volume = {44},
Number = {11},
Pages = {1107-1113},
Year = {1997},
Month = {November},
ISSN = {0018-9294},
url = {http://www.ncbi.nlm.nih.gov/pubmed/9353990},
Abstract = {A temperature control system consisting of a thermistor,
signal processor, and computer algorithm was developed for
magnetic resonance (MR) microscopy of small live animals.
With control of body temperature within +/- 0.2 degree C of
the set point, heart rate is stabilized and, in turn,
repetition time (TR) during cardiac-gated studies is less
variable. Thus, image quality and resolution are
improved.},
Doi = {10.1109/10.641338},
Key = {Qiu97}
}
@booklet{Sherrier85a,
Author = {SHERRIER, RH and SUDDARTH, SA and JOHNSON, GA and RAVIN,
CE},
Title = {AUTOMATED OBSERVER STUDIES ON A DIGITAL CHEST
SYSTEM},
Journal = {INVESTIGATIVE RADIOLOGY},
Volume = {20},
Number = {6},
Pages = {S18-S18},
Publisher = {LIPPINCOTT-RAVEN PUBL},
Year = {1985},
Month = {January},
ISSN = {0020-9996},
url = {http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:A1985ARG0500090&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=47d3190e77e5a3a53558812f597b0b92},
Key = {Sherrier85a}
}
@article{fds268883,
Author = {Ali, AA and Dale, AM and Badea, A and Johnson, GA},
Title = {Automated segmentation of neuroanatomical structures in
multispectral MR microscopy of the mouse
brain.},
Journal = {Neuroimage},
Volume = {27},
Number = {2},
Pages = {425-435},
Year = {2005},
Month = {August},
ISSN = {1053-8119},
url = {http://www.ncbi.nlm.nih.gov/pubmed/15908233},
Keywords = {Algorithms • Animals • Anisotropy • Bayes
Theorem • Brain • Image Processing,
Computer-Assisted • Magnetic Resonance Imaging •
Male • Markov Chains • Mice • Mice, Inbred
C57BL • Models, Statistical • Reproducibility of
Results • anatomy & histology* • methods* •
physiology* • statistics & numerical
data*},
Abstract = {We present the automated segmentation of magnetic resonance
microscopy (MRM) images of the C57BL/6J mouse brain into 21
neuroanatomical structures, including the ventricular
system, corpus callosum, hippocampus, caudate putamen,
inferior colliculus, internal capsule, globus pallidus, and
substantia nigra. The segmentation algorithm operates on
multispectral, three-dimensional (3D) MR data acquired at
90-microm isotropic resolution. Probabilistic information
used in the segmentation is extracted from training datasets
of T2-weighted, proton density-weighted, and
diffusion-weighted acquisitions. Spatial information is
employed in the form of prior probabilities of occurrence of
a structure at a location (location priors) and the pairwise
probabilities between structures (contextual priors).
Validation using standard morphometry indices shows good
consistency between automatically segmented and manually
traced data. Results achieved in the mouse brain are
comparable with those achieved in human brain studies using
similar techniques. The segmentation algorithm shows
excellent potential for routine morphological phenotyping of
mouse models.},
Doi = {10.1016/j.neuroimage.2005.04.017},
Key = {fds268883}
}
@article{fds268864,
Author = {Sharief, AA and Badea, A and Dale, AM and Johnson,
GA},
Title = {Automated segmentation of the actively stained mouse brain
using multi-spectral MR microscopy.},
Journal = {Neuroimage},
Volume = {39},
Number = {1},
Pages = {136-145},
Year = {2008},
Month = {January},
ISSN = {1053-8119},
url = {http://www.ncbi.nlm.nih.gov/pubmed/17933556},
Keywords = {Algorithms • Animals • Artificial Intelligence*
• Brain • Image Enhancement • Image
Interpretation, Computer-Assisted • Magnetic Resonance
Imaging • Male • Mice • Mice, Inbred C57BL
• Microscopy • Pattern Recognition, Automated
• Reproducibility of Results • Sensitivity and
Specificity • Staining and Labeling • Subtraction
Technique • cytology* • methods •
methods*},
Abstract = {Magnetic resonance microscopy (MRM) has created new
approaches for high-throughput morphological phenotyping of
mouse models of diseases. Transgenic and knockout mice serve
as a test bed for validating hypotheses that link genotype
to the phenotype of diseases, as well as developing and
tracking treatments. We describe here a Markov random fields
based segmentation of the actively stained mouse brain, as a
prerequisite for morphological phenotyping. Active staining
achieves higher signal to noise ratio (SNR) thereby enabling
higher resolution imaging per unit time than obtained in
previous formalin-fixed mouse brain studies. The
segmentation algorithm was trained on isotropic 43-mum T1-
and T2-weighted MRM images. The mouse brain was segmented
into 33 structures, including the hippocampus, amygdala,
hypothalamus, thalamus, as well as fiber tracts and
ventricles. Probabilistic information used in the
segmentation consisted of (a) intensity distributions in the
T1- and T2-weighted data, (b) location, and (c) contextual
priors for incorporating spatial information. Validation
using standard morphometric indices showed excellent
consistency between automatically and manually segmented
data. The algorithm has been tested on the widely used
C57BL/6J strain, as well as on a selection of six
recombinant inbred BXD strains, chosen especially for their
largely variant hippocampus.},
Doi = {10.1016/j.neuroimage.2007.08.028},
Key = {fds268864}
}
@article{fds174293,
Author = {GA Johnson and TR Hansen and KJ Austin and EA Van Kirk and WJ
Murdoch},
Title = {Baculovirus-insect cell production of bioactive
choriogonadotropin-immunoglobulin G heavy-chain fusion
proteins in sheep.},
Journal = {Biology of reproduction},
Volume = {52},
Number = {1},
Pages = {68-73},
Year = {1995},
Month = {January},
ISSN = {0006-3363},
Keywords = {Animals • Base Sequence • Cell Line •
Chorionic Gonadotropin • DNA Primers • DNA,
Complementary • Immunoglobulin G • Immunoglobulin
Heavy Chains • Male • Mice • Molecular
Sequence Data • Nucleopolyhedrovirus • Recombinant
Fusion Proteins • Sheep • Spodoptera • Testis
• Testosterone • Tumor Cells, Cultured •
biosynthesis • biosynthesis* • blood • drug
effects • genetics • pathology •
pharmacology},
Abstract = {A hybrid cDNA encoding a fusion protein between the beta
subunit of hCG (beta hCG) and constant domains of a mouse
IgG heavy chain (CH1-3) was inserted into a baculovirus
expression vector. Insect cells transfected with foreign DNA
synthesized multimeric forms of fusion protein that
inhibited hCG-induced steroid hormone secretion by mouse
Leydig tumor cells. Leydig cells were lysed by beta
hCG-CH1-3 in the presence of complement. Intravenous
injection of beta hCG-CH1-3 in rams was associated with
testicular mononuclear leukocyte infiltration, interstitial
tissue damage, and a transient depression in circulatory
testosterone (levels returned to normal within 2 wk). It
appears that targeted cell-killing can be mediated by
recombinant proteins composed of the receptor-binding moiety
of hormones and truncated effector (Fc) regions of lethal
antibodies.},
Language = {eng},
Key = {fds174293}
}
@booklet{Johnson95c,
Author = {G. A. Johnson and T. R. Hansen and K. J. Austin and E. A.
Vankirk and W. J. Murdoch},
Title = {Baculovirus-insect cell production of bioactive
choriogonadotropin-immunoglobulin-g heavy-chain fusion
proteins in sheep},
Journal = {Biology Of Reproduction},
Volume = {52},
Number = {1},
Pages = {68 -- 73},
Year = {1995},
Month = {January},
Key = {Johnson95c}
}
@article{fds292756,
Author = {Rajagopal, S and Kovacs, J and Badea, C and Johnson, GA and Rockman, HA and Piantadosi, CA and Lefkowitz, RJ},
Title = {BETA-ARRESTINS REGULATE SIGNALING BY BONE MORPHOGENETIC
PROTEIN TYPE II RECEPTOR IN PULMONARY ARTERIAL
HYPERTENSION},
Journal = {Journal of the American College of Cardiology},
Volume = {57},
Number = {14},
Pages = {E2046-E2046},
Publisher = {Elsevier BV},
Year = {2011},
Month = {April},
ISSN = {0735-1097},
url = {http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000291695102051&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=47d3190e77e5a3a53558812f597b0b92},
Doi = {10.1016/s0735-1097(11)62046-9},
Key = {fds292756}
}
@article{fds174135,
Author = {BM Markaverich and RR Roberts and MA Alejandro and GA Johnson and BS
Middleditch, JH Clark},
Title = {Bioflavonoid interaction with rat uterine type II binding
sites and cell growth inhibition.},
Journal = {Journal of steroid biochemistry},
Volume = {30},
Number = {1-6},
Pages = {71-8},
Year = {1988},
ISSN = {0022-4731},
Keywords = {Animals • Binding, Competitive • Breast Neoplasms
• Cell Division • Cell Line • Cell Nucleus
• Cytosol • Female • Flavonoids • Humans
• Kinetics • Rats • Receptors, Estradiol
• Receptors, Estrogen • Uterus • cytology
• drug effects • metabolism • metabolism*
• pharmacology*},
Abstract = {Competition analysis with a number of known bioflavonoids
demonstrated that these compounds (luteolin, quercetin,
pelargonin) compete for [3H]estradiol binding to cytosol and
nuclear type II sites in rat uterine preparations. The
inhibition of [3H]estradiol binding to type II sites was
specific and these bioflavonoids did not interact with the
rat uterine estrogen receptor. Since estradiol stimulation
of nuclear type II sites in the rat uterus is highly
correlated with cellular hypertrophy and hyperplasia, we
assessed the effects of these compounds on the growth of
MCF-7 human breast cancer cells in culture and on estradiol
stimulation of uterine growth in the immature rat. The data
demonstrated that addition of quercetin (5-10 micrograms/ml)
to MCF-7 cell cultures resulted in a dose-dependent
inhibition of cell growth (DNA/flask). This effect was
reversible by removal of quercetin from the culture medium,
or by the addition of 10 nM estradiol-17 beta to these cell
cultures containing this bioflavonoid. Since estradiol-17
beta (10 nM) stimulated nuclear type II sites and
proliferation of MCF-7 cells, we believe bioflavonoid
inhibition of MCF-7 cell growth may be mediated through an
interaction with nuclear type II sites. This hypothesis was
confirmed by in vivo studies which demonstrated that
injection of luteolin or quercetin blocked estradiol
stimulation of nuclear type II sites in the immature rat
uterus and this correlated with an inhibition of uterine
growth (wet and dry weight). These studies suggest
bioflavonoids, through an interaction with type II sites,
may be involved in cell growth regulation.},
Language = {eng},
Key = {fds174135}
}
@booklet{Markaverich88a,
Author = {B. M. Markaverich and R. R. Roberts and M. A. Alejandro and G. A. Johnson and B. S. Middleditch and J. H.
Clark},
Title = {Bioflavonoid interaction with rat uterine type-ii
binding-sites and cell-growth inhibition},
Journal = {Journal Of Steroid Biochemistry And Molecular
Biology},
Volume = {30},
Number = {1-6},
Pages = {71 -- 78},
Year = {1988},
Key = {Markaverich88a}
}
@booklet{Spencer03,
Author = {T. E. Spencer and R. C. Burghardt and G. A. Johnson and F.
W. Bazer},
Title = {Biology of progesterone and placental hormone actions on the
uterus.},
Journal = {Biology Of Reproduction},
Volume = {68},
Pages = {96 -- 97},
Year = {2003},
Key = {Spencer03}
}
@booklet{Teixeira97,
Author = {M. G. Teixeira and K. J. Austin and D. J. Perry and V. D.
Dooley and G. A. Johnson and B. R. Francis and T. R.
Hansen},
Title = {Bovine granulocyte chemotactic protein-2 is secreted by the
endometrium in response to interferon-tau
(IFN-tau)},
Journal = {Endocrine},
Volume = {6},
Number = {1},
Pages = {31 -- 37},
Year = {1997},
Month = {February},
Key = {Teixeira97}
}
@article{fds174211,
Author = {MG Teixeira and KJ Austin and DJ Perry and VD Dooley and GA Johnson and BR
Francis, TR Hansen},
Title = {Bovine granulocyte chemotactic protein-2 is secreted by the
endometrium in response to interferon-tau
(IFN-tau).},
Journal = {Endocrine},
Volume = {6},
Number = {1},
Pages = {31-7},
Year = {1997},
Month = {February},
ISSN = {1355-008X},
Keywords = {Amino Acid Sequence • Animals • Blotting, Western
• Cattle • Chemokine CXCL6 • Chemokines
• Chemokines, CXC* • Chromatography, Ion Exchange
• Electrophoresis, Polyacrylamide Gel •
Endometrium • Female • Interferon Type I •
Molecular Sequence Data • Pregnancy Proteins •
Sheep • immunology • metabolism*},
Abstract = {Interferon-tau (IFN-tau) is secreted by the bovine conceptus
and may regulate synthesis of uterine endometrial cytokines
to provide an environment that is conductive to embryo
development and implantation. Interferon-tau stimulates
secretion of an 8-kDa uterine protein (P8) in the cow. P8
was purified, digested to yield internal peptides, and
partially sequenced to determine identity. Two internal
peptides had 100% (13-mer) and 92% (12-mer) amino acid
sequence identity with bovine granulocyte chemotactic
protein-2 (bGCP-2). Bovine GCP-2 is an alpha-chemokine that
acts primarily as a potent chemoattractant for granulocyte
cells of the immune system. A peptide was synthesized based
on a region of bGCP-2 that overlapped with a P8 peptide
amino acid sequence, coupled to keyhole limpet hemocyanin,
and used to generate high titer polyclonal antiserum in
sheep. Western blots revealed that bGCP-2 was not released
by endometrium from day 14 nonpregnant cows, but was
released in response to 25 nM IFN-tau (p<0.05). Uterine
GCP-2 exhibited high affinity to heparin agarose, a
characteristic shared by all alpha chemokines. This is the
first report describing presence of GCP-2 in the uterine
endometrium and regulation by IFN-tau. The regulation of
bGCP-2 by IFN-tau may have important implications for
cytokine networking in the uterus during pregnancy. Also,
the regulation of inflammation and angiogenesis by bGCP-2
working together with other cytokines may be integral to
establishing early pregnancy and implantation in the
cow.},
Language = {eng},
Key = {fds174211}
}
@article{fds204273,
Author = {M Glaucia Teixeira and KJ Austin and DJ Perry and VD Dooley and GA
Johnson, BR Francis and TR Hansen},
Title = {Bovine granulocyte chemotactic protein-2 is secreted by the
endometrium in response to interferon-tau
(IFN-τ).},
Journal = {Endocrine},
Volume = {6},
Number = {1},
Pages = {31-7},
Year = {1997},
Month = {February},
ISSN = {1355-008X},
url = {http://dx.doi.org/10.1007/BF02738799},
Abstract = {Interferon-tau (IFN-τ) is secreted by the bovine conceptus
and may regulate synthesis of uterine endometrial cytokines
to provide an environment that is conducive to embryo
development and implantation. Interferon-τ stimulates
secretion of an 8-kDa uterine protein (P8) in the cow. P8
was purified, digested to yield internal peptides, and
partially sequenced to determine identity. Two internal
peptides had 100% (13-mer) and 92% (12-mer) amino acid
sequence identity with bovine granulocyte chemotactic
protein-2 (bGCP-2). Bovine GCP-2 is an α-chemokine that
acts primarily as a potent chemoattractant for granulocyte
cells of the immune system. A peptide was synthesized based
on a region of bGCP-2 that overlapped with a P8 peptide
amino acid sequence, coupled to keyhole limpet hemocyanin,
and used to generate high titer polyclonal antiserum in
sheep. Western blots revealed that bGCP-2 was not released
by endometrium from day 14 nonpregnant cows, but was
released in response to 25 nM IFN-τ (p<0.05). Uterine GCP-2
exhibited high affinity to heparin agarose, a characteristic
shared by all α chemokines. This is the first report
describing presence of GCP-2 in the uterine endometrium and
regulation by IFN-τ. The regulation of bGCP-2 by IFN-τ may
have important implications for cytokine networking in the
uterus during pregnancy. Also, the regulation of
inflammation and angiogenesis by bGCP-2 working together
with other cytokines may be integral to establishing early
pregnancy and implantation in the cow.},
Language = {eng},
Doi = {10.1007/BF02738799},
Key = {fds204273}
}
@booklet{Binelli01,
Author = {M. Binelli and P. Subramaniam and T. Diaz and G. A. Johnson and T. R. Hansen and L. Badinga and W. W.
Thatcher},
Title = {Bovine interferon-tau stimulates the Janus kinase-signal
transducer and activator of transcription pathway in bovine
endometrial epithelial cells},
Journal = {Biology Of Reproduction},
Volume = {64},
Number = {2},
Pages = {654 -- 665},
Year = {2001},
Month = {February},
Key = {Binelli01}
}
@article{fds174175,
Author = {M Binelli and P Subramaniam and T Diaz and GA Johnson and TR Hansen and L
Badinga, WW Thatcher},
Title = {Bovine interferon-tau stimulates the Janus kinase-signal
transducer and activator of transcription pathway in bovine
endometrial epithelial cells.},
Journal = {Biology of reproduction},
Volume = {64},
Number = {2},
Pages = {654-65},
Year = {2001},
Month = {February},
ISSN = {0006-3363},
Keywords = {Animals • Cattle • DNA-Binding Proteins •
Dimerization • Electrophoresis • Endometrium
• Epithelial Cells • Female • Immunoblotting
• Immunohistochemistry • Interferon Type I •
Janus Kinase 1 • Nuclear Proteins • Phenotype
• Phosphorylation • Precipitin Tests •
Pregnancy Proteins • Protein-Tyrosine Kinases •
STAT1 Transcription Factor • Signal Transduction •
Time Factors • Trans-Activators • cytology •
drug effects • drug effects* • enzymology •
genetics* • metabolism • metabolism* •
pharmacology*},
Abstract = {Trophoblastic bovine interferon-tau (bIFN-tau) suppresses
luteolytic pulses of endometrial prostaglandin F(2alpha)
(PGF(2alpha)) at the time of maternal recognition of
pregnancy. This results in maintenance of the corpus luteum
in cattle. The hypothesis that effects of bIFN-tau in the
endometrium were through activation of the Janus kinase
(JAK)-signal transducer and activator of transcription
(STAT) pathway of signal transduction was tested. Whole
cell, cytosolic, and nuclear extracts from bovine
endometrial cells treated with bIFN-tau were analyzed by
immunoprecipitation, immunoblotting, and electrophoretic
mobility shift assays in a series of dose- and
time-dependency experiments. Bovine IFN-tau stimulated
tyrosine phosphorylation, homo- and heterodimer formation,
nuclear translocation, and DNA binding of STAT proteins 1,
2, and 3. Moreover, bIFN-tau induced synthesis of
interferon-regulatory factor. In conclusion, bIFN-tau
stimulates the JAK-STAT pathway in the bovine endometrium.
It is proposed that activation of the JAK-STAT pathway is
involved in regulating the antiluteolytic effects of
bIFN-tau.},
Language = {eng},
Key = {fds174175}
}
@article{fds268766,
Author = {Bug, W and Wong, WW and Gustafson, C and Johnson, GA and Martone, ME and Price, DL and Rosen, GD and Williams, RW and Zaslavsky, I and Nissanov,
J},
Title = {Brain atlasing tool interoperation: NeuroTerrain-Smart Atlas
synergistic visualization and analysis environment},
Journal = {Proceedings of the 3rd International IEEE EMBS Conference on
Neural Engineering},
Pages = {280-283},
Publisher = {IEEE},
Year = {2007},
Month = {September},
ISBN = {9781424407927},
url = {http://dx.doi.org/10.1109/CNE.2007.369665},
Abstract = {Many research efforts using anatomical image analysis have
as their goal to identify biologically relevant objects
present within the image data, to provide a means to
quantitatively analyze these objects, to compare the
distribution of those objects to other features, and finally
to properly annotate the objects so as to be able share this
analysis in an integrated informatics framework. The
neuroinformatics tools designed to achieve these ends have
been developed in a fragmented manner with each resource
developing access to unique data sets and analytical
capabilities, Their integration would enrich the
neuroinformatic network enabling queries and analysis across
a number of resources. A central objective of the Biomedical
Informatics Research Network (BIRN) is to achieve this
integration and we present here a demonstration of how two
such tools - the NeuroTerrain Atlas/NetOStat client and the
SMART Atlas - can expose their functionality via a re-usable
interface, so as to promote interoperation of tools and
data. © 2007 IEEE.},
Doi = {10.1109/CNE.2007.369665},
Key = {fds268766}
}
@article{fds357209,
Author = {Blocker, SJ and Mowery, YM and Holbrook, MD and Qi, Y and Kirsch, DG and Johnson, GA and Badea, CT},
Title = {Bridging the translational gap: Implementation of multimodal
small animal imaging strategies for tumor burden assessment
in a co-clinical trial.},
Journal = {PLoS One},
Volume = {14},
Number = {4},
Pages = {e0207555},
Year = {2019},
url = {http://dx.doi.org/10.1371/journal.pone.0207555},
Abstract = {In designing co-clinical cancer studies, preclinical imaging
brings unique challenges that emphasize the gap between man
and mouse. Our group is developing quantitative imaging
methods for the preclinical arm of a co-clinical trial
studying immunotherapy and radiotherapy in a soft tissue
sarcoma model. In line with treatment for patients enrolled
in the clinical trial SU2C-SARC032, primary mouse sarcomas
are imaged with multi-contrast micro-MRI (T1 weighted, T2
weighted, and T1 with contrast) before and after immune
checkpoint inhibition and pre-operative radiation therapy.
Similar to the patients, after surgery the mice will be
screened for lung metastases with micro-CT using respiratory
gating. A systems evaluation was undertaken to establish a
quantitative baseline for both the MR and micro-CT systems
against which others systems might be compared. We have
constructed imaging protocols which provide
clinically-relevant resolution and contrast in a genetically
engineered mouse model of sarcoma. We have employed tools in
3D Slicer for semi-automated segmentation of both MR and
micro-CT images to measure tumor volumes efficiently and
reliably in a large number of animals. Assessment of tumor
burden in the resulting images was precise, repeatable, and
reproducible. Furthermore, we have implemented a publicly
accessible platform for sharing imaging data collected
during the study, as well as protocols, supporting
information, and data analyses. In doing so, we aim to
improve the clinical relevance of small animal imaging and
begin establishing standards for preclinical imaging of
tumors from the perspective of a co-clinical
trial.},
Doi = {10.1371/journal.pone.0207555},
Key = {fds357209}
}
@article{fds132744,
Author = {G.A. Johnson},
Title = {C. Zimmer, S.C.J. Wright, R.T. Engelhardt, G.A. Johnson,
X.O. Breakefield, R. Weissleder. Tumor cell endocytosi
(TCE) imaging facilitates delineation of the glioma-brain
interface. (1996).},
Year = {1996},
Key = {fds132744}
}
@booklet{Johnson03a,
Author = {G. A. Johnson and M. M. Joyce and S. Lewis and J. F.
Gonzalez and R. C. Burghardt and S. Woldesenbet and G. R.
Newton},
Title = {Caprine uterine and placental osteopontin (OPN) expression
is distinct among epitheliochorial implanting
species.},
Journal = {Biology Of Reproduction},
Volume = {68},
Pages = {205 -- 206},
Year = {2003},
Key = {Johnson03a}
}
@article{fds174218,
Author = {MM Joyce and JF González and S Lewis and S Woldesenbet and RC
Burghardt, GR Newton and GA Johnson},
Title = {Caprine uterine and placental osteopontin expression is
distinct among epitheliochorial implanting
species.},
Journal = {Placenta},
Volume = {26},
Number = {2-3},
Pages = {160-70},
Year = {2005},
Month = {July},
ISSN = {0143-4004},
url = {http://dx.doi.org/10.1016/j.placenta.2004.05.009},
Keywords = {Animals • Epithelial Cells • Female •
Gestational Age • Goats* • Molecular Probe
Techniques • Nucleic Acid Hybridization •
Osteopontin • Placenta • Pregnancy •
Pregnancy, Animal • RNA, Messenger • Sheep •
Sialoglycoproteins • Species Specificity • Swine
• Uterus • genetics • metabolism •
metabolism* • methods},
Abstract = {Osteopontin (OPN) is the most highly up-regulated
extracellular matrix/adhesion molecule in the uterus of
humans and domestic animals as it becomes receptive to
implantation. Studies in sheep and pigs have shown that OPN
is a component of ovine and porcine histotroph characterized
by a complex temporal and spatial pattern of uterine and
conceptus expression involving immune, epithelial, and
stromal cells. It is proposed that these expression events
are orchestrated to contribute to conceptus attachment and
placentation. However, differences in OPN expression between
sheep and pigs have been detected that relate to differences
in placentation. Therefore, this study examined OPN
expression in the caprine uterus and conceptus to gain
insight into mechanisms underlying OPN function(s) during
pregnancy through comparative analysis of differences in
placentation between pigs, sheep, and goats. Goats were
hysterectomized (n = 5/day) on Days 5, 11, 13, 15, 17 or 19
of the estrous cycle, and Days 5, 11, 13, 15, 17, 19 or 25
of pregnancy. Slot-blot hybridization showed increases in
endometrial OPN mRNA beginning on Day 17 of the estrous
cycle and Day 19 of pregnancy. In situ hybridization
localized OPN mRNA to endometrial glandular epithelium (GE),
Day 25 myometrium, and cells scattered within the placenta
hypothesized to be immune. Immunofluorescence microscopy
detected OPN protein on the apical surface of endometrial
lumenal epithelium (LE), in GE, and on conceptus (Tr).
Western blot analysis detected primarily the native 70-kDa
OPN protein in endometrial extracts from the estrous cycle
and pregnancy, as well as in uterine flushings from pregnant
goats. Co-induction of OPN and alpha-smooth muscle actin,
but not desmin proteins, was observed in uterine stroma by
Day 25 of pregnancy. OPN in cyclic GE, Day 25 myometrium,
and desmin-negative endometrial stroma is unique and
reflects subtle differences among superficial implanting
species that correlate with the depth of Tr
invasion.},
Language = {eng},
Doi = {10.1016/j.placenta.2004.05.009},
Key = {fds174218}
}
@article{fds174300,
Author = {TL Rutledge and MA Gold and DS McMeekin and WK Huh and MA Powell and SN
Lewin, DG Mutch and GA Johnson and JL Walker and RS
Mannel},
Title = {Carcinosarcoma of the ovary-a case series.},
Journal = {Gynecologic oncology},
Volume = {100},
Number = {1},
Pages = {128-32},
Year = {2006},
Month = {January},
ISSN = {0090-8258},
url = {http://dx.doi.org/10.1016/j.ygyno.2005.07.119},
Keywords = {Adult • Aged • Aged, 80 and over •
Antineoplastic Combined Chemotherapy Protocols •
Carboplatin • Carcinosarcoma • Chemotherapy,
Adjuvant • Cisplatin • Doxorubicin • Female
• Follow-Up Studies • Humans • Ifosfamide
• Middle Aged • Ovarian Neoplasms •
Paclitaxel • Proportional Hazards Models •
Retrospective Studies • Treatment Outcome •
administration & dosage • drug therapy* •
pathology • surgery* • therapeutic
use*},
Abstract = {OBJECTIVE: To evaluate our experience with ovarian
carcinosarcoma and identify prognostic factors. METHODS:
Thirty-one cases of ovarian carcinosarcoma were identified
over a 6-year time period through tumor registry and
pathology records. Fisher exact test and log rank using
Kaplan-Meier method (P < 0.05) were used to compare
variables with outcome. RESULTS: All 31 patients underwent
initial surgical treatment with an appropriate staging
procedure. Stage distribution: 1 stage I, 6 stage II, 23
stage III, and 1 stage IV. The median follow-up was 28
months. The median survival for the entire group was 21
months. Early vs. advanced stage significantly influenced
progression-free interval, P = 0.05. Nineteen patients were
found to have stage IIIC disease and required debulking
procedures. In patients with stage IIIC disease, presence of
residual disease was associated with decreased overall
survival, P = 0.03. 29 patients received adjuvant
chemotherapy with 11 patients receiving ifosfamide/cisplatin
and 16 patients receiving carboplatin/taxol.
Progression-free interval was improved with the use of
ifosfamide/cisplatin vs. carboplatin/taxol. The median PFI
was 12 months in the carbo/taxol group and has not been
reached in the ifos/cisplatin group (P = 0.005). The overall
survival was also significantly improved with the use of
ifosfamide/cisplatin, P = 0.03. In advanced stage patients,
overall survival was not significantly influenced by type of
adjuvant chemotherapy administered, P = 0.13. CONCLUSIONS:
Ovarian carcinosarcoma has a poor overall prognosis with
median survival rates reported in the literature ranging
from 7-10 months. Our series, although limited by a small
number of patients, exhibits a more encouraging median
survival of 21 months for the overall group. Aggressive
debulking to eliminate residual disease and the use of
ifosfamide/cisplatin chemotherapy seem to be factors in this
improved outcome.},
Language = {eng},
Doi = {10.1016/j.ygyno.2005.07.119},
Key = {fds174300}
}
@article{fds268856,
Author = {Badea, CT and Hedlund, LW and Mackel, JFB and Mao, L and Rockman, HA and Johnson, GA},
Title = {Cardiac micro-computed tomography for morphological and
functional phenotyping of muscle LIM protein null
mice.},
Journal = {Mol Imaging},
Volume = {6},
Number = {4},
Pages = {261-268},
Year = {2007},
ISSN = {1535-3508},
url = {http://www.ncbi.nlm.nih.gov/pubmed/17711781},
Keywords = {Animals • Calibration • Diastole •
Echocardiography • Heart Ventricles • Mice •
Mice, Inbred C57BL • Mice, Knockout • Muscle
Proteins • Myocardium • Phenotype •
Tomography, X-Ray Computed • deficiency* •
metabolism* • methods*},
Abstract = {The purpose of this study was to investigate the use of
micro-computed tomography (micro-CT) for morphological and
functional phenotyping of muscle LIM protein (MLP) null mice
and to compare micro-CT with M-mode echocardiography. MLP
null mice and controls were imaged using both micro-CT and
M-mode echocardiography. For micro-CT, we used a
custom-built scanner. Following a single intravenous
injection of a blood pool contrast agent (Fenestra VC, ART
Advanced Research Technologies, Saint-Laurent, QC) and using
a cardiorespiratory gating, we acquired eight phases of the
cardiac cycle (every 15 ms) and reconstructed
three-dimensional data sets with 94-micron isotropic
resolution. Wall thickness and volumetric measurements of
the left ventricle were performed, and cardiac function was
estimated. Micro-CT and M-mode echocardiography showed both
morphological and functional aspects that separate MLP null
mice from controls. End-diastolic and -systolic volumes were
increased significantly three- and fivefold, respectively,
in the MLP null mice versus controls. Ejection fraction was
reduced by an average of 32% in MLP null mice. The data
analysis shows that two imaging modalities provided
different results partly owing to the difference in
anesthesia regimens. Other sources of errors for micro-CT
are also analyzed. Micro-CT can provide the four-dimensional
data (three-dimensional isotropic volumes over time)
required for morphological and functional phenotyping in
mice.},
Doi = {10.2310/7290.2007.00022},
Key = {fds268856}
}
@article{fds268829,
Author = {Bucholz, E and Ghaghada, K and Qi, Y and Mukundan, S and Rockman, HA and Johnson, GA},
Title = {Cardiovascular phenotyping of the mouse heart using a 4D
radial acquisition and liposomal Gd-DTPA-BMA.},
Journal = {Magn Reson Med},
Volume = {63},
Number = {4},
Pages = {979-987},
Year = {2010},
Month = {April},
ISSN = {1522-2594},
url = {http://www.ncbi.nlm.nih.gov/pubmed/20373399},
Keywords = {Algorithms • Analysis of Variance • Animals •
Cardiovascular Physiological Phenomena* • Contrast
Media • Gadolinium DTPA • Imaging,
Three-Dimensional • Liposomes • Magnetic Resonance
Imaging • Mice • Mice, Inbred C57BL • Mice,
Inbred DBA • Phenotype • chemistry •
instrumentation • methods*},
Abstract = {MR microscopy has enormous potential for small-animal
cardiac imaging because it is capable of producing
volumetric images at multiple time points to accurately
measure cardiac function. MR has not been used as frequently
as ultrasound to measure cardiac function in the small
animal because the MR methods required relatively long scan
times, limiting throughput. Here, we demonstrate
four-dimensional radial acquisition in conjunction with a
liposomal blood pool agent to explore functional differences
in three populations of mice: six C57BL/6J mice, six DBA/2J
mice, and six DBA/2J CSQ+ mice, all with the same
gestational age and approximately the same weight.
Cardiovascular function was determined by measuring both
left ventricular and right ventricular end diastolic volume,
end systolic volume, stroke volume, and ejection fraction.
Statistical significance was observed in end diastolic
volume, end systolic volume, and ejection fraction for left
ventricular measurements between all three populations of
mice. No statistically significant difference was observed
in stroke volume in either the left or right ventricle for
any of the three populations of mice. This study shows that
MRI is capable of efficient, high-throughput,
four-dimensional cardiovascular phenotyping of the
mouse.},
Language = {eng},
Doi = {10.1002/mrm.22259},
Key = {fds268829}
}
@booklet{Heinz84,
Author = {HEINZ, ER and HERFKINS, R and JOHNSON, GA and DRAYER,
BP},
Title = {CAROTID-ARTERY IMAGING BY A 1.5-T MR SYSTEM},
Journal = {AMERICAN JOURNAL OF NEURORADIOLOGY},
Volume = {5},
Number = {5},
Pages = {670-670},
Publisher = {AMER SOC NEURORADIOLOGY},
Year = {1984},
Month = {January},
ISSN = {0195-6108},
url = {http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:A1984TG20900067&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=47d3190e77e5a3a53558812f597b0b92},
Key = {Heinz84}
}
@article{fds174172,
Author = {G Song and DW Bailey and KA Dunlap and RC Burghardt and TE Spencer and FW
Bazer, GA Johnson},
Title = {Cathepsin B, cathepsin L, and cystatin C in the porcine
uterus and placenta: potential roles in endometrial/placental
remodeling and in fluid-phase transport of proteins secreted
by uterine epithelia across placental areolae.},
Journal = {Biology of reproduction},
Volume = {82},
Number = {5},
Pages = {854-64},
Year = {2010},
Month = {May},
ISSN = {1529-7268},
url = {http://dx.doi.org/10.1095/biolreprod.109.080929},
Abstract = {Cathepsins (CTSB and CTSL1) and their inhibitor, cystatin C
(CST3), remodel uterine endometrium and placenta for
transport of gases, micronutrients, and macromolecules
essential for development and growth of the conceptus
(embryo/fetus and placental membranes). We examined the
temporal/spatial control of expression for CTSB, CTSL1, and
CST3 mRNAs in endometria and placentae of pigs using three
developmental models: 1) pigs were hysterectomized during
the estrous cycle or pregnancy; 2) cyclic pigs were injected
with estrogen to induce pseudopregnancy and were
hysterectomized; and 3) pigs were ovariectomized, injected
with progesterone, and hysterectomized. The abundance of
CTSB, CTSL1, and CST3 mRNAs increased in endometrial
epithelia during pregnancy and in response to exogenous
progesterone but not estrogen. CST3 was also expressed in
cells scattered within the stratum compactum stroma.
Progesterone decreased epithelial but increased stromal
compartment expression of CST3. CTSB increased in all
chorionic epithelia, but CTSL1 was limited to chorionic
epithelia that form areolae to absorb secretions from
uterine glands. Based on the placental and endometrial
distribution of CTSL1, we examined expression in the
neonatal enterocytes known to transport immunoglobulins from
colostrum. CTSL1 was also expressed in enterocytes of
intestine from neonatal piglets. Therefore, CTSL1 is
expressed by endometrial epithelia, placental areolae, and
neonatal intestine, and it may function in the transport of
macromolecules across these epithelia. Our results support
the idea that reciprocal interactions between CSTL1, CTSB,
and CST3 may be required to remodel endometrial and
placental tissues for close apposition between maternal and
fetal vasculatures and to facilitate transplacental
transport of gases, micronutrients (amino acids, glucose),
and macromolecules (proteins). Cysteine proteases and their
inhibitors may also specifically modify proteins for
successful utilization and fluid-phase transport across
uterine, placental, and neonatal gut epithelia.},
Language = {eng},
Doi = {10.1095/biolreprod.109.080929},
Key = {fds174172}
}
@booklet{Johnson87a,
Author = {G. A. Johnson},
Title = {Cbn wheel grinding},
Journal = {Journal Of Metals},
Volume = {39},
Number = {9},
Pages = {62 -- 63},
Year = {1987},
Month = {September},
Key = {Johnson87a}
}
@booklet{Benveniste99,
Author = {Benveniste, HD and Kim, KR and Hedlund, LW and Johnson, GA and Friedman,
AH},
Title = {Cerebral hemorrhage and edema following brain biopsy in
rats: Significance of mean arterial blood
pressure},
Journal = {ANESTHESIOLOGY},
Volume = {91},
Number = {3A},
Pages = {U371-U371},
Publisher = {LIPPINCOTT WILLIAMS & WILKINS},
Year = {1999},
Month = {September},
ISSN = {0003-3022},
url = {http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000082480600839&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=47d3190e77e5a3a53558812f597b0b92},
Key = {Benveniste99}
}
@article{fds174072,
Author = {JD Tobias and GA Johnson and S Rehman and R Fisher and N
Caron},
Title = {Cerebral oxygenation monitoring using near infrared
spectroscopy during one-lung ventilation in
adults.},
Journal = {Journal of minimal access surgery},
Volume = {4},
Number = {4},
Pages = {104-107},
Year = {2008},
Month = {October},
ISSN = {1998-3921},
Abstract = {BACKGROUND: Changes in oxygenation occur during one-lung
ventilation (OLV) due to intrapulmonary shunt. Although
arterial oxygenation is generally adequate, there are no
studies evaluating the effect of these changes on cerebral
oxygenation. MATERIALS AND METHODS: Cerebral oxygenation
(rSO(2)), heart rate (HR), blood pressure (BP), oxygen
saturation (SaO(2)), and end-tidal carbon dioxide (ETCO(2))
were prospectively monitored during OLV in adults. Cerebral
oxygenation was monitored using near infrared spectroscopy.
No clinical decisions were made based on the rSO2 value. BP
and HR were the inspired oxygen concentration was adjusted
as needed to maintain the SaO(2) >/= 95%. RESULTS: The study
cohort included 40 adult patients. 18,562 rSO(2) values were
collected during OLV. The rSO(2) was >/= baseline at 3,593
of the 18,562 data points (19%). The rSO2 was 0-9 </=
baseline in 7,053 (38%) of the readings, 10-19 </= baseline
in 4,084 (22%) of the readings, and 20-29 </= baseline in
3,898 (21%) of the readings. 2,599 (14%) of the rSO(2)
values were less than 75% of the baseline value. Thirteen
patients (32.5%) had at least one rSO2 value that was less
than 75% of the baseline. Eight patients (20%) had rSO(2)
values less than 75% of baseline for >/= 25% of the duration
of OLV. These patients were older (63.7 +/- 10.2 vs 54.6 +/-
9.8 years, P<0.025), weighed more (95.8 +/- 17.4 vs 82.6 +/-
14.6 kgs, P=0.038), and were more likely to be ASA III vs II
(7 of 8 versus 25 of 32, relative risk 1.75) than the
remainder of the cohort. CONCLUSIONS: Significant changes in
rSO2 occur during OLV for thoracic surgical procedures.
Future studies are needed to determine the impact of such
changes on the postoperative course of these
patients.},
Language = {ENG},
Key = {fds174072}
}
@article{fds204262,
Author = {GA Johnson},
Title = {Changes imposed on GPs make it harder to respond to
patients' needs.},
Journal = {BMJ (Clinical research ed.)},
Volume = {343},
Pages = {d7700},
Year = {2011},
ISSN = {1468-5833},
Keywords = {General Practice • Great Britain •
Physician-Patient Relations* • Quality Assurance,
Health Care* • State Medicine •
standards*},
Language = {eng},
Key = {fds204262}
}
@article{fds204272,
Author = {CL Gipson and GA Johnson and R Fisher and A Stewart and G Giles and JO
Johnson, JD Tobias},
Title = {Changes in cerebral oximetry during peritoneal insufflation
for laparoscopic procedures.},
Journal = {Journal of minimal access surgery},
Volume = {2},
Number = {2},
Pages = {67-72},
Year = {2006},
Month = {June},
ISSN = {0972-9941},
Abstract = {BACKGROUND: Changes in cardiac output may occur during
insufflation for laparoscopic procedures. However, there are
limited data regarding its potential effects on cerebral
oxygenation. METHODS: Cerebral oxygenation (ScO(2)), end
tidal CO(2), heart rate, blood pressure and oxygen
saturation by pulse oximetry were recorded every 5 minutes
prior to insufflation, during insufflation and after
desufflation. Minute ventilation was increased to maintain
normocapnia and the depth of anesthesia was adjusted or
fluids/phenylephrine administered to maintain the blood
pressure within 20% of the baseline. RESULTS: The cohort for
the study included 70 adults for laparoscopic herniorrhaphy,
gastric bypass or cholecystectomy. A total of 1004 ScO(2)
values were obtained during laparoscopy. The ScO(2)
decreased from the baseline in 758 of the 1004 data points.
The ScO(2) was 0-9 less than the baseline in 47.8% of the
values, 10-19 less than the baseline in 24.9% of the values
and 20-29 less than the baseline in 26 values (2.6%).
Eighty-two (8.2%) of the values were less than 80% of the
baseline value, while 25 values (2.5%) were less than 75% of
the baseline value. Twelve patients had at least one ScO(2)
value that was less than 80% of the baseline and 6 had at
least one ScO(2) value that was less than 75% of the
baseline. Four patients of the cohort had ScO(2) values less
than 80% of the baseline for more than 50% of the
laparoscopic procedure. CONCLUSIONS: Although relatively
uncommon, significant changes in cerebral oxygenation do
occur in some patients during insufflation for laparoscopic
surgery.},
Language = {eng},
Key = {fds204272}
}
@article{fds357203,
Author = {Wang, N and Mirando, AJ and Cofer, G and Qi, Y and Hilton, MJ and Johnson,
GA},
Title = {Characterization complex collagen fiber architecture in knee
joint using high-resolution diffusion imaging.},
Journal = {Magn Reson Med},
Volume = {84},
Number = {2},
Pages = {908-919},
Year = {2020},
Month = {August},
url = {http://dx.doi.org/10.1002/mrm.28181},
Abstract = {PURPOSE: To evaluate the complex fiber orientations and 3D
collagen fiber network of knee joint connective tissues,
including ligaments, muscle, articular cartilage, and
meniscus using high spatial and angular resolution diffusion
imaging. METHODS: Two rat knee joints were scanned using a
modified 3D diffusion-weighted spin echo pulse sequence with
the isotropic spatial resolution of 45 μm at 9.4T. The b
values varied from 250 to 1250 s/mm2 with 31 diffusion
encoding directions for 1 rat knee. The b value was fixed to
1000 s/mm2 with 147 diffusion encoding directions for the
second knee. Both the diffusion tensor imaging (DTI) model
and generalized Q-sampling imaging (GQI) method were used to
investigate the fiber orientation distributions and
tractography with the validation of polarized light
microscopy. RESULTS: To better resolve the crossing fibers,
the b value should be great than or equal to 1000 s/mm2 .
The tractography results were comparable between the DTI
model and GQI method in ligament and muscle. However, the
tractography exhibited apparent difference between DTI and
GQI in connective tissues with more complex collagen fibers
network, such as cartilage and meniscus. In articular
cartilage, there were numerous crossing fibers found in
superficial zone and transitional zone. Tractography
generated with GQI also resulted in more intact tracts in
articular cartilage than DTI. CONCLUSION: High-resolution
diffusion imaging with GQI method can trace the complex
collagen fiber orientations and architectures of the knee
joint at microscopic resolution.},
Doi = {10.1002/mrm.28181},
Key = {fds357203}
}
@article{fds268711,
Author = {Lipinski, RJ and Holloway, HT and O'Leary-Moore, SK and Ament, JJ and Pecevich, SJ and Cofer, GP and Budin, F and Everson, JL and Johnson, GA and Sulik, KK},
Title = {Characterization of subtle brain abnormalities in a mouse
model of Hedgehog pathway antagonist-induced cleft lip and
palate.},
Journal = {PLoS One},
Volume = {9},
Number = {7},
Pages = {e102603},
Year = {2014},
url = {http://hdl.handle.net/10161/11679 Duke open
access},
Abstract = {Subtle behavioral and cognitive deficits have been
documented in patient cohorts with orofacial clefts (OFCs).
Recent neuroimaging studies argue that these traits are
associated with structural brain abnormalities but have been
limited to adolescent and adult populations where brain
plasticity during infancy and childhood may be a confounding
factor. Here, we employed high resolution magnetic resonance
microscopy to examine primary brain morphology in a mouse
model of OFCs. Transient in utero exposure to the Hedgehog
(Hh) signaling pathway antagonist cyclopamine resulted in a
spectrum of facial dysmorphology, including unilateral and
bilateral cleft lip and palate, cleft of the secondary
palate only, and a non-cleft phenotype marked by midfacial
hypoplasia. Relative to controls, cyclopamine-exposed
fetuses exhibited volumetric differences in several brain
regions, including hypoplasia of the pituitary gland and
olfactory bulbs, hyperplasia of the forebrain septal region,
and expansion of the third ventricle. However, in affected
fetuses the corpus callosum was intact and normal division
of the forebrain was observed. This argues that
temporally-specific Hh signaling perturbation can result in
typical appearing OFCs in the absence of
holoprosencephaly--a condition classically associated with
Hh pathway inhibition and frequently co-occurring with OFCs.
Supporting the premise that some forms of OFCs co-occur with
subtle brain malformations, these results provide a possible
ontological basis for traits identified in clinical
populations. They also argue in favor of future
investigations into genetic and/or environmental modulation
of the Hh pathway in the etiopathogenesis of orofacial
clefting.},
Doi = {10.1371/journal.pone.0102603},
Key = {fds268711}
}
@booklet{Gerhards97,
Author = {R. Gerhards and D. Y. Wysepester and D. Mortensen and G. A.
Johnson},
Title = {Characterizing spatial stability of weed populations using
interpolated maps},
Journal = {Weed Science},
Volume = {45},
Number = {1},
Pages = {108 -- 119},
Year = {1997},
Key = {Gerhards97}
}
@article{fds174299,
Author = {MC DODD and NJ BIGLEY and GA JOHNSON and RH MCCLUER},
Title = {CHEMICAL ASPECTS ON INHIBITORS OF RH-O(D)
ANTIBODY.},
Journal = {Nature},
Volume = {204},
Pages = {549-52},
Year = {1964},
Month = {November},
ISSN = {0028-0836},
Keywords = {ANTIBODY FORMATION* • BRAIN CHEMISTRY* •
CHEMISTRY* • EXPERIMENTAL LAB STUDY* •
GANGLIOSIDES* • HEMAGGLUTINATION INHIBITION TESTS*
• PEPTIDES* • PSEUDOMONAS* • RABBITS* •
RH FACTORS*},
Language = {eng},
Key = {fds174299}
}
@article{fds132789,
Author = {CH Maynor and HC Charles and RJ Herfkens and SA Suddarth and GA
Johnson},
Title = {Chemical shift imaging of atherosclerosis at 7.0
Tesla.},
Journal = {Investigative radiology, UNITED STATES},
Volume = {24},
Number = {1},
Pages = {52-60},
Year = {1989},
Month = {January},
ISSN = {0020-9996},
Keywords = {Adipose Tissue • Aged • Aorta • Aortic
Diseases • Arteriosclerosis • Cholesterol Esters
• Female • Humans • Magnetic Resonance
Spectroscopy • Male • Middle Aged •
Triglycerides • diagnostic use* • metabolism
• pathology • pathology*},
Abstract = {Chemical shift imaging (CSI) was performed on cadaveric
atherosclerotic fibrous plaques, periaortic adipose tissue,
and cholesterol standards using a 7.0 Tesla horizontal bore
prototype imaging spectrometer. Proton spectroscopy of
intact tissue and deuterated chloroform extracted samples
was done at the equivalent field strength of 7.0 Tesla on a
vertical bore spectrometer, including studies of temperature
dependence and T2 relaxation measurements. Spectra obtained
using CSI on the imaging magnet were comparable with those
from the conventional vertical spectrometer. Fibrous plaques
and adipose tissue had unique spectral features, differing
in the ratios of their water and various fat components.
Chloroform extractions revealed a typical cholesteric ester
spectrum for the fibrous plaque in contrast to the
triglyceride spectrum of the adipose tissue. These two
tissues also had different T2 relaxation measurements of
their major fat resonances, with fibrous plaques having a
short T2 compared to adipose tissue (15.9 milliseconds vs.
46.2 milliseconds). Temperature dependence studies showed
that spectral signal intensity of the fat resonance of the
fibrous plaque increased while linewidth decreased with
increasing temperature from 24 degrees C to 37 degrees C.
Atherosclerotic lesions may be studied at 7.0 Tesla, and NMR
parameters defined in the present study may be used for
further studies at other magnetic field strengths.},
Key = {fds132789}
}
@booklet{Maynor89,
Author = {Maynor, CH and Charles, HC and Herfkens, RJ and Suddarth, SA and Johnson, GA},
Title = {Chemical shift imaging of atherosclerosis at 7.0
Tesla.},
Journal = {Invest Radiol},
Volume = {24},
Number = {1},
Pages = {52-60},
Year = {1989},
Month = {January},
ISSN = {0020-9996},
url = {http://www.ncbi.nlm.nih.gov/pubmed/2917823},
Abstract = {Chemical shift imaging (CSI) was performed on cadaveric
atherosclerotic fibrous plaques, periaortic adipose tissue,
and cholesterol standards using a 7.0 Tesla horizontal bore
prototype imaging spectrometer. Proton spectroscopy of
intact tissue and deuterated chloroform extracted samples
was done at the equivalent field strength of 7.0 Tesla on a
vertical bore spectrometer, including studies of temperature
dependence and T2 relaxation measurements. Spectra obtained
using CSI on the imaging magnet were comparable with those
from the conventional vertical spectrometer. Fibrous plaques
and adipose tissue had unique spectral features, differing
in the ratios of their water and various fat components.
Chloroform extractions revealed a typical cholesteric ester
spectrum for the fibrous plaque in contrast to the
triglyceride spectrum of the adipose tissue. These two
tissues also had different T2 relaxation measurements of
their major fat resonances, with fibrous plaques having a
short T2 compared to adipose tissue (15.9 milliseconds vs.
46.2 milliseconds). Temperature dependence studies showed
that spectral signal intensity of the fat resonance of the
fibrous plaque increased while linewidth decreased with
increasing temperature from 24 degrees C to 37 degrees C.
Atherosclerotic lesions may be studied at 7.0 Tesla, and NMR
parameters defined in the present study may be used for
further studies at other magnetic field strengths.},
Doi = {10.1097/00004424-198901000-00012},
Key = {Maynor89}
}
@article{fds132773,
Author = {GA Johnson and GW Dewald and WR Strand and RK Winkelmann},
Title = {Chromosome studies in 17 patients with the Sézary
syndrome.},
Journal = {Cancer, UNITED STATES},
Volume = {55},
Number = {10},
Pages = {2426-33},
Year = {1985},
Month = {May},
ISSN = {0008-543X},
Keywords = {Adult • Aged • Chromosome Aberrations •
Chromosomes • Female • Genetic Markers •
Humans • Karyotyping • Male • Metaphase
• Middle Aged • Sezary Syndrome • genetics*
• mortality • pathology •
ultrastructure*},
Abstract = {Chromosome studies were done on phytohemagglutinin-stimulated
peripheral blood from 17 patients with Sézary syndrome. A
chromosomally abnormal clone was found in five patients:
each patient had an abnormal chromosome 6 and four had an
abnormal chromosome 1. Six patients without abnormal clones
had more than 20% metaphases with random heteroploidy and
sporadic structural anomalies. Only normal metaphases were
seen in four patients, and no metaphases were found in two.
Four of the five patients with an abnormal clone died, and
their median survival from chromosome analysis was 6 months;
only one of these patients died of lymphoma. The six
patients with increased heteroploidy had long survivals and
no apparent malignant process. Two of the four patients with
normal metaphases died of malignant disease: one had
lymphoma and the other squamous cell carcinoma. A third
patient with normal chromosomes died of extensive visceral
cutaneous T-cell lymphoma.},
Key = {fds132773}
}
@booklet{Johnson85b,
Author = {G. A. Johnson and G. W. Dewald and W. R. Strand and R. K.
Winkelmann},
Title = {Chromosome-studies in 17 patients with the sezary
syndrome},
Journal = {Cancer},
Volume = {55},
Number = {10},
Pages = {2426 -- 2433},
Year = {1985},
Key = {Johnson85b}
}
@article{fds132907,
Author = {AC Brau and LW Hedlund and GA Johnson},
Title = {Cine magnetic resonance microscopy of the rat heart using
cardiorespiratory-synchronous projection
reconstruction.},
Journal = {Journal of magnetic resonance imaging : JMRI, United
States},
Volume = {20},
Number = {1},
Pages = {31-8},
Year = {2004},
Month = {July},
ISSN = {1053-1807},
Keywords = {Animals • Artifacts • Female • Image
Processing, Computer-Assisted* • Magnetic Resonance
Imaging, Cine* • Microscopy • Myocardial
Contraction* • Rats • Rats, Sprague-Dawley •
Respiration* • methods},
Abstract = {PURPOSE: To tailor a cardiac magnetic resonance (MR)
microscopy technique for the rat that combines improvements
in pulse sequence design and physiologic control to acquire
high-resolution images of cardiac structure and function.
MATERIALS AND METHODS: Projection reconstruction (PR) was
compared to conventional Cartesian techniques in
point-spread function simulations and experimental studies
to evaluate its artifact sensitivity. Female Sprague-Dawley
rats were imaged at 2.0 T using PR with direct encoding of
the free induction decay. Specialized physiologic support
and monitoring equipment ensured consistency of biological
motion and permitted synchronization of imaging with the
cardiac and respiratory cycles. RESULTS: The reduced
artifact sensitivity of PR offered improved delineation of
cardiac and pulmonary structures. Ventilatory
synchronization further increased the signal-to-noise ratio
by reducing inter-view variability. High-quality short-axis
and long-axis cine images of the rat heart were acquired
with 10-msec temporal resolution and microscopic spatial
resolution down to 175 microm x 175 microm x 1 mm.
CONCLUSION: Integrating careful biological control with an
optimized pulse sequence significantly limits both the
source and impact of image artifacts. This work represents a
novel integration of techniques designed to support
measurement of cardiac morphology and function in rodent
models of cardiovascular disease.},
Key = {fds132907}
}
@booklet{Brau04,
Author = {Brau, ACS and Hedlund, LW and Johnson, GA},
Title = {Cine magnetic resonance microscopy of the rat heart using
cardiorespiratory-synchronous projection
reconstruction.},
Journal = {J Magn Reson Imaging},
Volume = {20},
Number = {1},
Pages = {31-38},
Year = {2004},
Month = {July},
ISSN = {1053-1807},
url = {http://www.ncbi.nlm.nih.gov/pubmed/15221806},
Abstract = {PURPOSE: To tailor a cardiac magnetic resonance (MR)
microscopy technique for the rat that combines improvements
in pulse sequence design and physiologic control to acquire
high-resolution images of cardiac structure and function.
MATERIALS AND METHODS: Projection reconstruction (PR) was
compared to conventional Cartesian techniques in
point-spread function simulations and experimental studies
to evaluate its artifact sensitivity. Female Sprague-Dawley
rats were imaged at 2.0 T using PR with direct encoding of
the free induction decay. Specialized physiologic support
and monitoring equipment ensured consistency of biological
motion and permitted synchronization of imaging with the
cardiac and respiratory cycles. RESULTS: The reduced
artifact sensitivity of PR offered improved delineation of
cardiac and pulmonary structures. Ventilatory
synchronization further increased the signal-to-noise ratio
by reducing inter-view variability. High-quality short-axis
and long-axis cine images of the rat heart were acquired
with 10-msec temporal resolution and microscopic spatial
resolution down to 175 microm x 175 microm x 1 mm.
CONCLUSION: Integrating careful biological control with an
optimized pulse sequence significantly limits both the
source and impact of image artifacts. This work represents a
novel integration of techniques designed to support
measurement of cardiac morphology and function in rodent
models of cardiovascular disease.},
Doi = {10.1002/jmri.20089},
Key = {Brau04}
}
@booklet{Fleming01,
Author = {J. A. G. W. Fleming and Y. Choi and G. A. Johnson and T. E.
Spencer and F. W. Bazer},
Title = {Cloning of the ovine estrogen receptor-alpha promoter and
functional regulation by ovine interferon-tau},
Journal = {Endocrinology},
Volume = {142},
Number = {7},
Pages = {2879 -- 2887},
Year = {2001},
Month = {July},
Key = {Fleming01}
}
@article{fds174194,
Author = {JA Fleming and Y Choi and GA Johnson and TE Spencer and FW
Bazer},
Title = {Cloning of the ovine estrogen receptor-alpha promoter and
functional regulation by ovine interferon-tau.},
Journal = {Endocrinology},
Volume = {142},
Number = {7},
Pages = {2879-87},
Year = {2001},
Month = {July},
ISSN = {0013-7227},
Keywords = {Animals • Artificial Gene Fusion • Base Sequence
• Cell Line, Transformed • Cloning, Molecular*
• DNA-Binding Proteins • Electrophoresis •
Endometrium • Enhancer Elements, Genetic •
Epithelial Cells • Estrogen Receptor alpha •
Female • Gene Deletion • Interferon Regulatory
Factor-2 • Interferon Type I • Mutation •
Pregnancy Proteins • Promoter Regions, Genetic •
Receptors, Estrogen • Recombinant Proteins •
Repressor Proteins* • Sheep • Thymidine Kinase
• Transcription Factors* • Transcription, Genetic
• cytology • drug effects • genetics •
genetics* • metabolism • metabolism* •
pharmacology • physiology*},
Abstract = {Interferon-tau (IFNtau), the ruminant pregnancy recognition
signal, inhibits transcription of the estrogen receptor
alpha (ERalpha) gene in the endometrial lumenal epithelium
of the sheep uterus, thereby abrogating production of
luteolytic PGF(2alpha) pulses. The effects of IFNtau are
mediated in part by IFN-stimulated response elements (ISREs)
and IFN regulatory factor elements (IRFEs). The
promoter/enhancer region of the ovine ERalpha gene was
cloned, sequenced, and predicted to contain four IRFEs and
one ISRE. Electrophoretic mobility shift assays indicated
that the -2110 IRFE bound only IRF-1, whereas the -1877 IRFE
and the -1284 ISRE were functional in binding IRF-1 and
IRF-2. IFNtau inhibited transcriptional activity of the
2.7-kb ovine ERalpha promoter in transfection assays using
ovine lumenal epithelium cells. Analyses of sequential
5'-deletion mutants of the ovine ERalpha promoter indicated
that the effects of IFNtau may be mediated by IRFEs as well
as other elements. Overexpression of ovine IRF-2, but not
IRF-1, inhibited transcriptional activity of several regions
of the ovine ERalpha promoter containing an IRFE or an ISRE
as well as some, but not all, regions lacking these
elements.},
Language = {eng},
Key = {fds174194}
}
@article{fds132916,
Author = {GA Johnson and J Baker},
Title = {Colonic perforation following mild trauma in a patient with
Crohn's disease.},
Journal = {The American journal of emergency medicine, UNITED
STATES},
Volume = {8},
Number = {4},
Pages = {340-1},
Year = {1990},
Month = {July},
ISSN = {0735-6757},
Keywords = {Adult • Colon • Crohn Disease • Humans •
Intestinal Perforation • Male • complications*
• etiology* • injuries* • pathology •
surgery},
Abstract = {A 26-year-old man with a history of Crohn's disease was
struck in the abdomen by an opponent's shoulder while
playing basketball. He presented to the emergency department
3 hours later with the complaint of abdominal pain and was
admitted to the hospital for observation. Nine hours after
presentation a computed tomography scan showed he had
pneumoperitoneum and then underwent laparotomy. A perforated
segment of sigmoid colon with severe inflammatory disease
was found and resected. The rest of his small and large
bowels were otherwise unremarkable. His localized but severe
inflammatory bowel disease predisposed him to bowel
perforation with minimal trauma. This is the first report of
a patient with inflammatory bowel disease and traumatic
colon perforation; it is also the first report of a patient
with a bowel perforation with minimal traumatic
force.},
Key = {fds132916}
}
@booklet{Johnson90a,
Author = {G. A. Johnson and J. Baker},
Title = {Colonic perforation following mild trauma in a patient with
crohns-disease},
Journal = {American Journal Of Emergency Medicine},
Volume = {8},
Number = {4},
Pages = {340 -- 341},
Year = {1990},
Month = {July},
Key = {Johnson90a}
}
@booklet{Fischer04,
Author = {D. W. Fischer and R. G. Harvey and T. T. Bauman and S.
Phillips and S. E. Hart and G. A. Johnson and J. J. Kells and P. Westra and J. Lindquist},
Title = {Common lambsquarters (Chenopodium album) interference with
corn across the northcentral United States},
Journal = {Weed Science},
Volume = {52},
Number = {6},
Pages = {1034 -- 1038},
Year = {2004},
Key = {Fischer04}
}
@article{fds174261,
Author = {FW Bazer and TE Spencer and GA Johnson and RC Burghardt and G
Wu},
Title = {Comparative aspects of implantation.},
Journal = {Reproduction (Cambridge, England)},
Volume = {138},
Number = {2},
Pages = {195-209},
Year = {2009},
Month = {August},
ISSN = {1741-7899},
url = {http://dx.doi.org/10.1530/REP-09-0158},
Keywords = {Animals • Blastocyst • Embryo Implantation •
Endometrium • Female • Gene Expression Regulation,
Developmental* • Interferons • Pregnancy •
Pregnancy, Animal • Primates • Progesterone •
Rats • Species Specificity • Swine •
metabolism • physiology*},
Abstract = {Uterine receptivity to implantation of blastocysts in
mammals includes hatching from zona pellucida, precontact
with uterine luminal (LE) and superficial glandular (sGE)
epithelia and orientation of blastocyst, apposition between
trophectoderm and uterine LE and sGE, adhesion of
trophectoderm to uterine LE/sGE, and, in some species,
limited or extensive invasion into the endometrial stroma
and induction of decidualization of stromal cells. These
peri-implantation events are prerequisites for pregnancy
recognition signaling, implantation, and placentation
required for fetal-placental growth and development through
the remainder of pregnancy. Although there is a range of
strategies for implantation in mammals, a common feature is
the requirement for progesterone (P(4)) to downregulate
expression of its receptors in uterine epithelia and P(4)
prior to implantation events. P(4) then mediates its effects
via growth factors expressed by stromal cells in most
species; however, uterine luminal epithelium may express a
growth factor in response to P(4) and/or estrogens in
species with a true epitheliochorial placenta. There is also
compelling evidence that uterine receptivity to implantation
involves temporal and cell-specific expression of interferon
(IFN)-stimulated genes that may be induced directly by an
IFN or induced by P(4) and stimulated by an IFN. These genes
have many roles including nutrient transport, cellular
remodeling, angiogenesis and relaxation of vascular tissues,
cell proliferation and migration, establishment of an
antiviral state, and protection of conceptus tissues from
challenges by the maternal immune cells.},
Language = {eng},
Doi = {10.1530/REP-09-0158},
Key = {fds174261}
}
@article{fds174139,
Author = {GA Johnson and EG Kim and PA Platz and MM Mickelson},
Title = {Comparative aspects of tyrosine hydroxylase and tryptophan
hydroxylase inhibition: arterenones and dihydroxyphenylacetamide
(H 22-54).},
Journal = {Biochemical pharmacology},
Volume = {17},
Number = {3},
Pages = {403-10},
Year = {1968},
Month = {March},
ISSN = {0006-2952},
Keywords = {Acetophenones • Adrenal Medulla • Amides •
Animals • Brain • Cattle • Iron •
Mast-Cell Sarcoma • Methyltyrosines • Mice •
Mixed Function Oxygenases* • Norepinephrine •
Phenylacetates* • Phenylalanine • Sarcoma,
Experimental • Tritium • Tryptophan •
Tyrosine • antagonists & inhibitors • enzymology
• enzymology* • metabolism • pharmacology
• pharmacology*},
Language = {eng},
Key = {fds174139}
}
@article{fds174246,
Author = {D Robertson and GA Johnson and RM Robertson and AS Nies and DG Shand and JA
Oates},
Title = {Comparative assessment of stimuli that release neuronal and
adrenomedullary catecholamines in man.},
Journal = {Circulation},
Volume = {59},
Number = {4},
Pages = {637-43},
Year = {1979},
Month = {April},
ISSN = {0009-7322},
Keywords = {Adolescent • Adrenal Medulla • Adult • Blood
Specimen Collection • Caffeine • Catecholamines
• Epinephrine • Female • Humans •
Isometric Contraction • Male • Middle Aged •
Neurons • Norepinephrine • Physical Stimulation
• Posture • Pressoreceptors • Valsalva
Maneuver • blood • pharmacology •
physiology*},
Abstract = {We assessed the release of neuronal and adrenomedullary
catecholamines in response to various stimuli of the
sympathetic nervous system in normal subjects. Plasma
catecholamines and their urinary metabolites,
normetanephrine and metanephrine, were measured. Sodium
restriction increased supine plasma norepinephrine by 37%
and ambulatory plasma norepinephrine by 22%, with urinary
normetanephrine excretion increased 29%. The sodium
restriction did not elevate plasma epinephrine or urinary
metanephrine. The most potent stimuli of norepinephrine were
treadmill exercise, orthostasis, caffeine, the cold pressor
test, sodium restriction and handgrip exercise, in
descending order. Plasma epinephrine was increased by
caffeine, treadmill exercise, the cold pressor test,
handgrip exercise and the Valsalva maneuver, in that order.
Syncope resulted in profound changes in plasma epinephrine
but only modest changes in plasma norepinephrine. We
conclude that in man, there is frequent dissociation between
the effects of different stimuli on neuronal and
adrenomedullary catecholamine release.},
Language = {eng},
Key = {fds174246}
}
@booklet{Macfall91,
Author = {MACFALL, JS and JOHNSON, GA and KRAMER, PJ},
Title = {Comparative water uptake by roots of different ages in
seedlings of loblolly pine (Pinus taeda L.)},
Journal = {New Phytologist},
Volume = {119},
Number = {4},
Pages = {551-560},
Publisher = {WILEY},
Year = {1991},
Month = {January},
ISSN = {0028-646X},
url = {http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:A1991HA17900010&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=47d3190e77e5a3a53558812f597b0b92},
Abstract = {Magnetic resonance (MR) imaging was used to study water
absorption from fine, moist sand by the taproot,
first‐order lateral roots, and tine roots of
9‐month‐old loblolly pine seedlings. Magnetic resonance
imaging provides the opportunity for repeated
non‐destructive measurements of water uptake by roots
growing in a solid medium such as sand. Root systems of
container‐grown seedlings were pruned to a taproot, one or
two first‐order laterals and attached fine roots, and were
planted in small containers. Reference tubes filled with a
mixture of CuSO4/D2O were placed in each container in the
field of view. Roots of individual seedlings were repeatedly
imaged at approximately 3 h intervals. Water uptake by
individual roots was measured and uptake was calculated
based on biomass, root length, and surface area. Based on
weight and surface area, but not on root length, fine roots
were more efficient than the lateral or taproots in water
uptake. Measurement of water content in MR images of wet
sand was confirmed by the imaging of Sand phantoms. These
phantoms were tubes filled with fine sand at varied water
contents (5–25%). Additional tubes of CuSO4 with D2O were
also imaged. A linear relationship between signal intensity
of moist sand (normalized against the CuSO4/D2O) and water
conteni was demonstrated (R2= 0.97). Fitting the normalized
signal intensity of the sand to the calculated linear
regression allowed calculation of the water content of the
sand. Copyright © 1991, Wiley Blackwell. All rights
reserved},
Doi = {10.1111/j.1469-8137.1991.tb01047.x},
Key = {Macfall91}
}
@booklet{Wong92,
Author = {W. W. Wong and L. L. Clarke and G. A. Johnson and M.
Llaurador and P. D. Klein},
Title = {Comparison of 2 elemental-analyzer gas-isotope-ratio
mass-spectrometer systems in the simultaneous measurement of
c-13/c-12 ratios and carbon content in organic-samples},
Journal = {Analytical Chemistry},
Volume = {64},
Number = {4},
Pages = {354 -- 358},
Year = {1992},
Month = {February},
Key = {Wong92}
}
@article{fds268729,
Author = {Befera, NT and Badea, CT and Johnson, GA},
Title = {Comparison of 4D-microSPECT and microCT for murine cardiac
function.},
Journal = {Mol Imaging Biol},
Volume = {16},
Number = {2},
Pages = {235-245},
Year = {2014},
Month = {April},
url = {http://www.ncbi.nlm.nih.gov/pubmed/24037175},
Abstract = {PURPOSE: The objective of this study was to compare a new
generation of four-dimensional micro-single photon emission
computed tomography (microSPECT) with microCT for the
quantitative in vivo assessment of murine cardiac function.
PROCEDURES: Four-dimensional isotropic cardiac images were
acquired from anesthetized normal C57BL/6 mice with either
microSPECT (n = 6) or microCT (n = 6). One additional mouse
with myocardial infarction (MI) was scanned with both
modalities. Prior to imaging, mice were injected with either
technetium tetrofosmin for microSPECT or a liposomal blood
pool contrast agent for microCT. Segmentation of the left
ventricle (LV) was performed using Vitrea (Vital Images)
software, to derive global and regional function. RESULTS:
Measures of global LV function between microSPECT and
microCT groups were comparable (e.g., ejection fraction = 71
± 6 % microSPECT and 68 ± 4 % microCT). Regional
functional indices (wall motion, wall thickening, regional
ejection fraction) were also similar for the two modalities.
In the mouse with MI, microSPECT identified a large
perfusion defect that was not evident with microCT.
CONCLUSIONS: Despite lower spatial resolution, microSPECT
was comparable to microCT in the quantitative evaluation of
cardiac function. MicroSPECT offers an advantage over
microCT in the ability to evaluate simultaneously myocardial
radiotracer distribution and function, simultaneously.
MicroSPECT should be considered as an alternative to microCT
and magnetic resonance for preclinical cardiac imaging in
the mouse.},
Doi = {10.1007/s11307-013-0686-z},
Key = {fds268729}
}
@booklet{Staggs98,
Author = {K. L. Staggs and K. J. Austin and G. A. Johnson and M. G.
Teixeira and C. T. Talbott and V. A. Dooley and T. R.
Hansen},
Title = {Complex induction of bovine uterine proteins by
interferon-tau},
Journal = {Biology Of Reproduction},
Volume = {59},
Number = {2},
Pages = {293 -- 297},
Year = {1998},
Month = {August},
Key = {Staggs98}
}
@article{fds132910,
Author = {KL Staggs and KJ Austin and GA Johnson and MG Teixeira and CT Talbott and VA Dooley and TR Hansen},
Title = {Complex induction of bovine uterine proteins by
interferon-tau.},
Journal = {Biology of reproduction},
Volume = {59},
Number = {2},
Pages = {293-7},
Year = {1998},
Month = {August},
ISSN = {0006-3363},
Keywords = {Animals • Blotting, Western • Cattle • Cells,
Cultured • Chemokine CXCL6 • Chemokines, CXC
• Chromatography, Affinity • Endometrium •
Epithelial Cells • Estrus • Female •
Interferon Type I • Interferon Type I, Recombinant
• Pregnancy • Pregnancy Proteins • Protein
Biosynthesis* • Stimulation, Chemical • Ubiquitins
• Uterus • biosynthesis • drug effects •
metabolism • metabolism* • pharmacology •
pharmacology* • physiology},
Abstract = {Interferon-tau (IFN-tau) is released by the conceptus and
induces two uterine proteins during early pregnancy:
ubiquitin cross-reactive protein (UCRP) and granulocyte
chemotactic protein-2 (GCP-2). The present experiments were
designed to determine whether detection (Western blot) of
cytosolic UCRP and release of GCP-2 could be used to examine
IFN-tau signal transduction in cultured endometrial explants
and primary epithelial cells. Recombinant (r) type 1 IFNs
(rboIFN-tau and rboIFN-alpha; 5, 25, 100 nM) induced UCRP,
but only rboIFN-tau induced GCP-2 in explant culture.
Recombinant boIFN-tau and conceptus secretory proteins
containing native IFN-tau induced UCRP and GCP-2 in cultured
primary epithelial cells. All concentrations of rboIFN-alpha
(25, 50, 100 nM) induced UCRP, but only the highest
concentration induced GCP-2 in cultured primary epithelial
cells. Interestingly, phorbol ester (100, 500, 1000 ng/ml)
induced GCP-2, but it had no effect on UCRP. Because type 1
IFNs induce UCRP, IFN-tau probably interacts with the janus
kinase (Jak)-associated IFN-alpha receptor to phosphorylate
signal transducers and activators of transcription (STAT)
and/or interferon regulatory factor-1 (IRF-1). However,
IFN-tau-specific induction of GCP-2 may involve a variant
type 1 receptor subunit or activators of transcription that
are associated with protein kinase C and the Jak/STAT/IRF-1
pathway.},
Language = {eng},
Key = {fds132910}
}
@article{fds174148,
Author = {B Govenar and M Freeman and DC Bergquist and GA Johnson and CR
Fisher},
Title = {Composition of a one-year-old Riftia pachyptila community
following a clearance experiment: insight to succession
patterns at deep-sea hydrothermal vents.},
Journal = {The Biological bulletin},
Volume = {207},
Number = {3},
Pages = {177-82},
Year = {2004},
Month = {December},
ISSN = {0006-3185},
Keywords = {Animals • Biomass • Ecosystem* • Environment*
• Invertebrates • Pacific Ocean • Population
Dynamics • Species Specificity • growth &
development*},
Language = {eng},
Key = {fds174148}
}
@booklet{Brown87,
Author = {Brown, JM and Fonteno, WC and Cassel, DK and Johnson,
GA},
Title = {COMPUTED TOMOGRAPHIC ANALYSES OF WATER DISTRIBUTION IN THREE
POROUS FOAM MEDIA.},
Journal = {Soil Science Society of America Journal},
Volume = {51},
Number = {5},
Pages = {1121-1125},
Publisher = {Soil Science Society of America},
Year = {1987},
Month = {January},
url = {http://dx.doi.org/10.2136/sssaj1987.03615995005100050005x},
Abstract = {Computer Assisted Tomography (CAT) is commonly used in
diagnostic radiology to make nondestructive images and
analyses of cross sections of the human body. CAT scanning
may also be useful in imaging and measuring spatial
distribution and changes in water distribution in porous
media. The purpose of this paper is to review some of the
details of CAT scanning that are of importance to the
application of CAT scanning to porous media and to evaluate
the use of the CAT scanner to measure the spatial
distribution of water in three different porous media. The
scanner's response to changes in the spatial distribution of
water in three different porous phenolic foam materials
after draining for 16 h was investigated. Water content
distributions were successfully detected with good
resolution on the x-ray image.},
Doi = {10.2136/sssaj1987.03615995005100050005x},
Key = {Brown87}
}
@article{fds268789,
Author = {Badea, CT and Athreya, KK and Espinosa, G and Clark, D and Ghafoori, AP and Li, Y and Kirsch, DG and Johnson, GA and Annapragada, A and Ghaghada,
KB},
Title = {Computed tomography imaging of primary lung cancer in mice
using a liposomal-iodinated contrast agent.},
Journal = {PLoS One},
Volume = {7},
Number = {4},
Pages = {e34496},
Year = {2012},
url = {http://www.ncbi.nlm.nih.gov/pubmed/22485175},
Abstract = {PURPOSE: To investigate the utility of a liposomal-iodinated
nanoparticle contrast agent and computed tomography (CT)
imaging for characterization of primary nodules in
genetically engineered mouse models of non-small cell lung
cancer. METHODS: Primary lung cancers with mutations in
K-ras alone (Kras(LA1)) or in combination with p53
(LSL-Kras(G12D);p53(FL/FL)) were generated. A
liposomal-iodine contrast agent containing 120 mg Iodine/mL
was administered systemically at a dose of 16 µl/gm body
weight. Longitudinal micro-CT imaging with
cardio-respiratory gating was performed pre-contrast and at
0 hr, day 3, and day 7 post-contrast administration.
CT-derived nodule sizes were used to assess tumor growth.
Signal attenuation was measured in individual nodules to
study dynamic enhancement of lung nodules. RESULTS: A good
correlation was seen between volume and diameter-based
assessment of nodules (R(2)>0.8) for both lung cancer
models. The LSL-Kras(G12D);p53(FL/FL) model showed rapid
growth as demonstrated by systemically higher volume changes
compared to the lung nodules in Kras(LA1) mice (p<0.05).
Early phase imaging using the nanoparticle contrast agent
enabled visualization of nodule blood supply. Delayed-phase
imaging demonstrated significant differential signal
enhancement in the lung nodules of LSL-Kras(G12D);p53(FL/FL)
mice compared to nodules in Kras(LA1) mice (p<0.05)
indicating higher uptake and accumulation of the
nanoparticle contrast agent in rapidly growing nodules.
CONCLUSIONS: The nanoparticle iodinated contrast agent
enabled visualization of blood supply to the nodules during
the early-phase imaging. Delayed-phase imaging enabled
characterization of slow growing and rapidly growing nodules
based on signal enhancement. The use of this agent could
facilitate early detection and diagnosis of pulmonary
lesions as well as have implications on treatment response
and monitoring.},
Doi = {10.1371/journal.pone.0034496},
Key = {fds268789}
}
@booklet{Cassel90,
Author = {Cassel, DK and Brown, JM and Johnson, GA},
Title = {Computer tomographic analysis of water distribution and flow
in porous media},
Journal = {Theoretical and Applied Climatology},
Volume = {42},
Number = {4},
Pages = {223-228},
Publisher = {Springer Nature},
Year = {1990},
Month = {December},
ISSN = {0177-798X},
url = {http://dx.doi.org/10.1007/BF00865982},
Abstract = {Computer tomography (CT) is the reconstruction by computer
of a tomographic plane (slice) of an object. The tomograph
is developed from multiple X-ray absorption measurements
(scans) made around the periphery of the object. Recent
research in soil science indicates that CT, which has been
used in the medical field for 17 years, may find
applications in assessing the degree of uniformity, or lack
thereof, of soils and other porous media, in determining the
flow paths of water and solutes through soils and porous
materials, and in determing the flow paths of water and
solutes to roots of plants. This paper discusses the general
concept of CT, some of the capabilities associated with
software used to format the CT readings into the desired
images, and presents some CT scan data for several draining
porous media. © 1990 Springer-Verlag.},
Doi = {10.1007/BF00865982},
Key = {Cassel90}
}
@article{fds268746,
Author = {HEDLUND, L and JONES, D and EFFMANN, E and JOHNSON, GA and BATES, W and WOLFE, W and PUTMAN, C},
Title = {COMPUTERIZED TOMOGRAPHIC STUDY OF THE DOG LUNG DURING
HEMORRHAGIC-SHOCK},
Journal = {INVESTIGATIVE RADIOLOGY},
Volume = {14},
Number = {5},
Pages = {369-369},
Publisher = {LIPPINCOTT-RAVEN PUBL},
Year = {1979},
Month = {January},
ISSN = {0020-9996},
url = {http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:A1979HQ94500028&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=47d3190e77e5a3a53558812f597b0b92},
Key = {fds268746}
}
@booklet{Joyce04,
Author = {M. M. Joyce and R. C. Burghardt and T. E. Spencer and G. A.
Johnson},
Title = {Conceptus secretory factors, other than estrogen, increase
expression of interferon-stimulated genes in the porcine
endometrium during pregnancy.},
Journal = {Biology Of Reproduction},
Pages = {211 -- 211},
Year = {2004},
Key = {Joyce04}
}
@article{fds157088,
Author = {TE Spencer and RC Burghardt and GA Johnson and FW
Bazer},
Title = {Conceptus signals for establishment and maintenance of
pregnancy.},
Journal = {Animal reproduction science},
Volume = {82-83},
Pages = {537-50},
Year = {2004},
Month = {July},
ISSN = {0378-4320},
url = {http://dx.doi.org/10.1016/j.anireprosci.2004.04.014},
Keywords = {Animals • Animals, Domestic • Corpus Luteum •
Female • Fetus • Interferons • Pregnancy
• Pregnancy Maintenance • Progesterone •
Sheep • Signal Transduction* • Swine •
Trophoblasts • metabolism • physiology •
physiology*},
Abstract = {Establishment and maintenance of pregnancy results from
signaling by the conceptus (embryo/fetus and associated
extraembryonic membranes) and requires progesterone produced
by the corpus luteum. In most mammals, hormones produced by
the trophoblast maintain progesterone production by acting
directly or indirectly to maintain the corpus luteum. In
domestic animals (ruminants and pigs), hormones from the
trophoblast are antiluteolytic in that they act on the
endometrium to prevent uterine release of luteolytic
prostaglandin F2alpha. In cyclic and pregnant sheep,
progesterone negatively autoregulates progesterone receptor
gene expression in the endometrial luminal and superficial
glandular epithelium. In cyclic sheep, loss of the
progesterone receptor is closely followed by increases in
epithelial estrogen receptors and then oxytocin receptors,
allowing oxytocin to induce uterine release of luteolytic
prostaglandin F2alpha pulses. In pregnant sheep, the
conceptus trophoblast produces interferon tau that acts on
the endometrium to inhibit transcription of the estrogen
receptor alpha gene directly and the oxytocin receptor gene
indirectly to abrogate development of the endometrial
luteolytic mechanism. Subsequently, sequential, overlapping
actions of progesterone, interferon tau, placental lactogen,
and growth hormone comprise a hormonal servomechanism that
regulates endometrial gland morphogenesis and terminal
differentiated function to maintain pregnancy in sheep. In
pigs, the conceptus trophoblast produces estrogen that
alters the direction of prostaglandin F2alpha secretion from
an endocrine to exocrine direction, thereby sequestering
luteolytic prostaglandin F2alpha within the uterine lumen.
Conceptus estrogen also increases expression of fibroblast
growth factor 7 in the endometrial lumenal epithelium that,
in turn, stimulates proliferation and differentiated
functions of the trophectoderm, which expresses the
fibroblast growth factor 7 receptor. Strategic manipulation
of these physiological mechanisms may improve uterine
capacity, conceptus survival, and reproductive
health.},
Language = {eng},
Doi = {10.1016/j.anireprosci.2004.04.014},
Key = {fds157088}
}
@article{fds174111,
Author = {GA Johnson and FW Bazer and RC Burghardt and TE Spencer and G Wu and KJ
Bayless},
Title = {Conceptus-uterus interactions in pigs: endometrial gene
expression in response to estrogens and interferons from
conceptuses.},
Journal = {Society of Reproduction and Fertility supplement},
Volume = {66},
Pages = {321-32},
Year = {2009},
Keywords = {Animals • Embryo Implantation • Embryo, Mammalian
• Endometrium • Estrogens • Female •
Gene Expression Regulation, Developmental • Interferons
• Pregnancy • Swine • physiology •
physiology*},
Abstract = {This review highlights information on conceptus-uterus
interactions in the pig with respect to uterine gene
expression in response to estrogens and interferons (IFNs)
secreted from elongating conceptuses. Pig conceptuses
release estrogens for pregnancy recognition, but also
secrete IFNs that do not appear to be antiluteolytic.
Estrogens and IFNs induce expression of largely
non-overlapping sets of genes, and evidence suggests that
pig conceptuses orchestrate essential events of early
pregnancy including pregnancy recognition signaling,
implantation and secretion of histotroph by precisely
controlling temporal and spatial (cell-specific) changes in
uterine gene expression through initial secretion of
estrogens, followed by cytokines including IFNG and IFND. By
Day 12 of pregnancy, estrogens increase the expression of
multiple genes in the uterine luminal epithelium including
SPP1, STC1, IRF2 and STAT1 that likely have roles for
implantation. By Day 15 of pregnancy, IFNs upregulate a
large array of IFN responsive genes in the underlying stroma
and glandular epithelium including ISG15, IRF1, STAT1, SLAs
and B2M that likely have roles in uterine remodeling to
support placentation.},
Language = {eng},
Key = {fds174111}
}
@article{fds204271,
Author = {D Srinivasan and N Muthukrishnan and GA Johnson and A Erazo-Oliveras and J Lim and EE Simanek and JP Pellois},
Title = {Conjugation to the cell-penetrating peptide TAT potentiates
the photodynamic effect of carboxytetramethylrhodamine.},
Journal = {PloS one},
Volume = {6},
Number = {3},
Pages = {e17732},
Year = {2011},
ISSN = {1932-6203},
url = {http://dx.doi.org/10.1371/journal.pone.0017732},
Keywords = {Amino Acid Sequence • Animals • Carotenoids •
Cell Death • Cell Line • Cell Membrane • Cell
Membrane Permeability • Cell-Penetrating Peptides
• Drug Synergism • Endocytosis • Endosomes
• Humans • Light • Molecular Sequence Data
• Photochemotherapy* • Photolysis • Protein
Processing, Post-Translational • Rhodamines •
Singlet Oxygen • chemistry • drug effects •
metabolism • metabolism* • pharmacology •
radiation effects • therapeutic use*},
Abstract = {BACKGROUND: Cell-penetrating peptides (CPPs) can transport
macromolecular cargos into live cells. However, the cellular
delivery efficiency of these reagents is often suboptimal
because CPP-cargo conjugates typically remain trapped inside
endosomes. Interestingly, irradiation of fluorescently
labeled CPPs with light increases the release of the peptide
and its cargos into the cytosol. However, the mechanism of
this phenomenon is not clear. Here we investigate the
molecular basis of the photo-induced endosomolytic activity
of the prototypical CPPs TAT labeled to the fluorophore
5(6)-carboxytetramethylrhodamine (TMR). RESULTS: We report
that TMR-TAT acts as a photosensitizer that can destroy
membranes. TMR-TAT escapes from endosomes after exposure to
moderate light doses. However, this is also accompanied by
loss of plasma membrane integrity, membrane blebbing, and
cell-death. In addition, the peptide causes the destruction
of cells when applied extracellularly and also triggers the
photohemolysis of red blood cells. These photolytic and
photocytotoxic effects were inhibited by hydrophobic singlet
oxygen quenchers but not by hydrophilic quenchers.
CONCLUSIONS: Together, these results suggest that TAT can
convert an innocuous fluorophore such as TMR into a potent
photolytic agent. This effect involves the targeting of the
fluorophore to cellular membranes and the production of
singlet oxygen within the hydrophobic environment of the
membranes. Our findings may be relevant for the design of
reagents with photo-induced endosomolytic activity. The
photocytotoxicity exhibited by TMR-TAT also suggests that
CPP-chromophore conjugates could aid the development of
novel Photodynamic Therapy agents.},
Language = {eng},
Doi = {10.1371/journal.pone.0017732},
Key = {fds204271}
}
@booklet{Walker93a,
Author = {L. C. Walker and S. Murad and A. G. Messenger and G. A.
Johnson and S. R. Pinnell},
Title = {Connective-tissue effects of minoxidil and its hydroxy
derivatives on human dermal papilla cells in
culture},
Journal = {Clinical Research},
Volume = {41},
Number = {2},
Pages = {A437 -- A437},
Year = {1993},
Month = {April},
Key = {Walker93a}
}
@booklet{Walker93,
Author = {L. C. Walker and S. Murad and A. G. Messenger and G. A.
Johnson and S. R. Pinnell},
Title = {Connective-tissue effects of minoxidil and its hydroxy
derivatives on human dermal papilla cells in
culture},
Journal = {Journal Of Investigative Dermatology},
Volume = {100},
Number = {4},
Pages = {552 -- 552},
Year = {1993},
Month = {April},
Key = {Walker93}
}
@article{fds268730,
Author = {Clark, D and Badea, A and Johnson, GA and Badea, CT},
Title = {Constructing a 4D murine cardiac micro-CT atlas for
automated segmentation and phenotyping applications},
Journal = {Progress in Biomedical Optics and Imaging - Proceedings of
SPIE},
Volume = {8669},
Publisher = {SPIE},
Year = {2013},
Month = {June},
ISSN = {1605-7422},
url = {http://dx.doi.org/10.1117/12.2007043},
Abstract = {A number of investigators have demonstrated the potential of
preclinical micro-CT in characterizing cardiovascular
disease in mouse models. One major hurdle to advancing this
approach is the extensive user interaction required to
derive quantitative metrics from these 4D image arrays
(space + time). In this work, we present: (1) a method for
constructing an average anatomic cardiac atlas of the mouse
based on 4D micro-CT images, (2) a fully automated approach
for segmenting newly acquired cardiac data sets using the
atlas, and (3) a quantitative characterization of atlasbased
segmentation accuracy and consistency. Employing the
deformable registration toolkit, ANTs, the construction of
minimal deformation fields, and a novel adaptation of joint
bilateral filtration, our atlas construction scheme was used
to integrate 6, C57BL/6 cardiac micro-CT data sets, reducing
the noise standard deviation from ~70 HU in the individual
data sets to ~21 HU in the atlas data set. Using the
segmentation tools in Atropos and our atlas-based
segmentation, we were able to propagate manual labels to 5,
C57BL/6 data sets not used in atlas construction. Average
Dice coefficients and volume accuracies (respectively) over
phases 1 (ventricular diastole), 3, and 5 (ventricular
systole) of these 5 data sets were as follows: left
ventricle, 0.96, 0.96; right ventricle, 0.89, 0.92; left
atrium, 0.88, 0.89; right atrium, 0.86, 0.92; myocardium,
0.90, 0.94. Once the atlas was constructed and segmented,
execution of the proposed automated segmentation scheme took
~6.5 hours per data set, versus more than 50 hours required
for a manual segmentation. © 2013 SPIE.},
Doi = {10.1117/12.2007043},
Key = {fds268730}
}
@booklet{Shengunther97,
Author = {J. Shengunther and R. S. Mannel and J. L. Walker and G. A.
Johnson},
Title = {Construction of the ileocolonic continent urinary reservoir:
Operative technique at the University of
Oklahoma},
Journal = {Journal Of Gynecologic Surgery},
Volume = {13},
Number = {2},
Pages = {83 -- 88},
Year = {1997},
Key = {Shengunther97}
}
@article{fds268742,
Author = {Peterson, RA and Gabrielson, KL and Allan Johnson and G and Pomper, MG and Coatney, RW and Winkelmann, CT},
Title = {Continuing education course #1: non-invasive imaging as a
problem-solving tool and translational biomarker strategy in
toxicologic pathology.},
Journal = {Toxicol Pathol},
Volume = {39},
Number = {1},
Pages = {267-272},
Year = {2011},
Month = {January},
ISSN = {0192-6233},
url = {http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000293379600029&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=47d3190e77e5a3a53558812f597b0b92},
Abstract = {The continuing education course "Non-Invasive Imaging as a
Problem-Solving Tool and Translational Biomarker Strategy in
Toxicologic Pathology" provided a thorough overview of
commonly used imaging modalities and the logistics required
for integration of small animal imaging into toxicologic
pathology. Non-invasive imaging (NIN) is gaining acceptance
as an important modality in toxicologic pathology. This
technology allows nonterminal, time-course evaluation of
functional and morphologic endpoints and can be used to
translate biomarkers between preclinical animal models and
human patients. NIN can support drug development as well as
basic research in academic or industrial environments. An
initial overview of theoretical principles was followed by
focused presentations on magnetic resonance imaging
(MRI)/magnetic resonance microscopy (MRM), positron emission
tomography (PET)/single proton emission computed tomography
(SPECT), ultrasonography (US, primarily focused on
echocardiography), optical (bioluminescent) imaging, and
computed tomography (CT). The choice of imaging modality
will depend on the research question and the needed
resolution.},
Doi = {10.1177/0192623310390392},
Key = {fds268742}
}
@article{fds268770,
Author = {Bolon, B and Garman, RH and Gundersen, HJG and Allan Johnson and G and Kaufmann, W and Krinke, G and Little, PB and Makris, SL and Mellon, RD and Sulik, KK and Jensen, K},
Title = {Continuing education course #3: current practices and future
trends in neuropathology assessment for developmental
neurotoxicity testing.},
Journal = {Toxicol Pathol},
Volume = {39},
Number = {1},
Pages = {289-293},
Year = {2011},
Month = {January},
ISSN = {1533-1601},
url = {http://dx.doi.org/10.1177/0192623310386247},
Abstract = {The continuing education course on Developmental
Neurotoxicity Testing (DNT) was designed to communicate
current practices for DNT neuropathology, describe promising
innovations in quantitative analysis and noninvasive
imaging, and facilitate a discussion among experienced
neuropathologists and regulatory scientists regarding
suitable DNT practices. Conventional DNT neuropathology
endpoints are qualitative histopathology and morphometric
endpoints of particularly vulnerable sites (e.g., cerebral,
cerebellar, or hippocampal thickness). Novel imaging and
stereology measurements hold promise for automated analysis
of factors that cannot be effectively examined in routinely
processed specimens (e.g., cell numbers, fiber tract
integrity). The panel recommended that dedicated DNT
neuropathology data sets be acquired on a minimum of 8
sections (for qualitative assessment) or 3 sections (for
quantitative linear and stereological analyses) using a
small battery of stains to examine neurons and myelin. Where
guidelines permit discretion, immersion fixation is
acceptable for younger animals (postnatal day 22 or
earlier), and peripheral nerves may be embedded in paraffin.
Frequent concerns regarding DNT data sets include
false-negative outcomes due to processing difficulties
(e.g., lack of concordance among sections from different
animals) and insensitive analytical endpoints (e.g.,
qualitative evaluation) as well as false-positive results
arising from overinterpretation or misreading by
inexperienced pathologists.},
Doi = {10.1177/0192623310386247},
Key = {fds268770}
}
@booklet{Halvorsen82,
Author = {Halvorsen, RA and Woodfield, S and Hedlund, L and Johnson, GA and Thompson, WM},
Title = {CONTRAST ENHANCEMENT OF THE HEPATOBILIARY SYSTEM DURING
CT},
Journal = {Investigative Radiology},
Volume = {17},
Number = {4},
Pages = {S27-S27},
Publisher = {Ovid Technologies (Wolters Kluwer Health)},
Year = {1982},
Month = {July},
ISSN = {0020-9996},
url = {http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:A1982PA96700126&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=47d3190e77e5a3a53558812f597b0b92},
Doi = {10.1097/00004424-198207000-00134},
Key = {Halvorsen82}
}
@booklet{Thompson83,
Author = {Thompson, WM and Stude, R and Bates, M and Hedlund, L and Johnson,
GA},
Title = {CONTRAST ENHANCEMENT OF THE LIVER DURING
CT},
Journal = {Investigative Radiology},
Volume = {18},
Number = {4},
Pages = {S11-S11},
Publisher = {Ovid Technologies (Wolters Kluwer Health)},
Year = {1983},
Month = {July},
ISSN = {0020-9996},
url = {http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:A1983QZ74200056&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=47d3190e77e5a3a53558812f597b0b92},
Doi = {10.1097/00004424-198307000-00064},
Key = {Thompson83}
}
@booklet{Stude87,
Author = {R. A. Stude and L. W. Hedlund and G. A. Johnson and W. M.
Thompson},
Title = {Contrast enhancement of the liver evaluation by automated
contiguous pixel search},
Journal = {Investigative Radiology},
Volume = {22},
Number = {2},
Pages = {132 -- 136},
Year = {1987},
Month = {February},
Key = {Stude87}
}
@article{fds268928,
Author = {Stude, RA and Hedlund, LW and Johnson, GA and Thompson,
WM},
Title = {Contrast enhancement of the liver. Evaluation by automated
contiguous pixel search.},
Journal = {Invest Radiol},
Volume = {22},
Number = {2},
Pages = {132-136},
Year = {1987},
Month = {February},
ISSN = {0020-9996},
url = {http://www.ncbi.nlm.nih.gov/pubmed/3030962},
Keywords = {Animals • Contrast Media* • Diatrizoate Meglumine
• Dogs • Ethiodized Oil • Liver •
Radiographic Image Enhancement* • Tomography, X-Ray
Computed* • diagnostic use • radiography*},
Abstract = {Eight dogs were infused with one of three different contrast
agents: meglumine diatrizoate, iosulamide meglumine, or an
ethiodized oil emulsion (EOE 13). Image intensity in the
liver was evaluated globally and regionally by means of an
automated pixel sampling method to determine differences in
the rate of enhancement produced by the three agents. The
results of the automated method were compared with those of
the standard manual cursor method. The automated method
showed that liver parenchyma was enhanced uniformly by all
three contrast agents. The maximum degree of enhancement
(Mean +/- SEM) for the three agents was diatrizoate, 12.0
+/- 3.5 Hounsfield units (HU); iosluamide, 21.3 +/- 3.5 HU;
and EOE 13, 37.2 +/- 4.25 HU. With the manual cursor method,
contrast enhancement was about 20% more than estimated by
the automated method. The automated method is better for
evaluating the magnitude and pattern of contrast agent
enhancement of the entire liver, since the currently
employed cursor technique requires multiple evaluations to
evaluate the entire liver.},
Doi = {10.1097/00004424-198702000-00008},
Key = {fds268928}
}
@article{fds268824,
Author = {Howles, GP and Bing, KF and Qi, Y and Rosenzweig, SJ and Nightingale,
KR and Johnson, GA},
Title = {Contrast-enhanced in vivo magnetic resonance microscopy of
the mouse brain enabled by noninvasive opening of the
blood-brain barrier with ultrasound.},
Journal = {Magn Reson Med},
Volume = {64},
Number = {4},
Pages = {995-1004},
Year = {2010},
Month = {October},
ISSN = {1522-2594},
url = {http://www.ncbi.nlm.nih.gov/pubmed/20740666},
Keywords = {Animals • Blood-Brain Barrier • Brain •
Contrast Media • Fluorocarbons • Gadolinium DTPA
• Image Enhancement • Magnetic Resonance Imaging
• Mice • Mice, Inbred C57BL • Microscopy
• Sonication • anatomy & histology* •
diagnostic use • diagnostic use* • metabolism*
• methods • methods* • pharmacokinetics
• pharmacokinetics* • radiation
effects},
Abstract = {The use of contrast agents for neuroimaging is limited by
the blood-brain barrier (BBB), which restricts entry into
the brain. To administer imaging agents to the brain of
rats, intracarotid infusions of hypertonic mannitol have
been used to open the BBB. However, this technically
challenging approach is invasive, opens only a limited
region of the BBB, and is difficult to extend to mice. In
this work, the BBB was opened in mice, using unfocused
ultrasound combined with an injection of microbubbles. This
technique has several notable features: it (a) can be
performed transcranially in mice; (b) takes only 3 min and
uses only commercially available components; (c) opens the
BBB throughout the brain; (d) causes no observed histologic
damage or changes in behavior (with peak-negative acoustic
pressures of 0.36 MPa); and (e) allows recovery of the BBB
within 4 h. Using this technique, Gadopentetate Dimeglumine
(Gd-DTPA) was administered to the mouse brain parenchyma,
thereby shortening T(1) and enabling the acquisition of
high-resolution (52 × 52 × 100 micrometers(3)) images in
51 min in vivo. By enabling the administration of both
existing anatomic contrast agents and the newer
molecular/sensing contrast agents, this technique may be
useful for the study of mouse models of neurologic function
and pathology with MRI.},
Language = {eng},
Doi = {10.1002/mrm.22411},
Key = {fds268824}
}
@booklet{Sills04,
Author = {Sills, RC and Morgan, DL and Herr, DW and Little, PB and George, NM and Ton, TV and Love, NE and Maronpot, RR and Johnson,
GA},
Title = {Contribution of magnetic resonance microscopy in the 12-week
neurotoxicity evaluation of carbonyl sulfide in Fischer 344
rats.},
Journal = {Toxicol Pathol},
Volume = {32},
Number = {5},
Pages = {501-510},
Year = {2004},
url = {http://dx.doi.org/10.1080/01926230490493918},
Abstract = {In this carbonyl sulfide (COS) study, magnetic resonance
microscopy (MRM) and detailed light microscopic evaluation
effectively functioned in parallel to assure that the
distribution and degree of pathology in the brain was
accurately represented. MRM is a powerful imaging modality
that allows for excellent identification of neuroanatomical
structures coupled with the ability to acquire 200 or more
cross-sectional images of the brain, and the ability to
display them in multiple planes. F344 rats were exposed to
200-600 ppm COS for up to 12 weeks. Prior to MRM, rats were
anesthetized and cardiac perfused with McDowell Trump's
fixative containing a gadolinium MR contrast medium. Fixed
specimens were scanned at the Duke Center for In Vivo
Microscopy on a 9.4 Tesla magnetic resonance system adapted
explicitly for microscopic imaging. An advantage of MRM in
this study was the ability to identify lesions in rats that
appeared clinically normal prior to sacrifice and the
opportunity to identify lesions in areas of the brain which
would not be included in conventional studies. Other
advantages include the ability to examine the brain in
multiple planes (transverse, dorsal, sagittal) and obtain
and save the MRM images in a digital format that allows for
postexperimental data processing and manipulation. MRM
images were correlated with neuroanatomical and
neuropathological findings. All suspected MRM images were
compared to corresponding H&E slides. An important aspect of
this study was that MRM was critical in defining our
strategy for sectioning the brain, and for designing
mechanistic studies (cytochrome oxidase evaluations) and
functional assessments (electrophysiology studies) on
specifically targeted anatomical sites following COS
exposure.},
Doi = {10.1080/01926230490493918},
Key = {Sills04}
}
@article{fds132895,
Author = {RC Sills and DL Morgan and DW Herr and PB Little and NM George and TV Ton and NE Love and RR Maronpot and GA Johnson},
Title = {Contribution of magnetic resonance microscopy in the 12-week
neurotoxicity evaluation of carbonyl sulfide in Fischer 344
rats.},
Journal = {Toxicologic pathology, United States},
Volume = {32},
Number = {5},
Pages = {501-10},
ISSN = {0192-6233},
Keywords = {Administration, Inhalation • Air Pollutants •
Animals • Brain • Dose-Response Relationship, Drug
• Female • Image Processing, Computer-Assisted
• Inhalation Exposure • Magnetic Resonance
Imaging* • Male • Microscopy • Neurotoxicity
Syndromes • Rats • Rats, Inbred F344 •
Recovery of Function • Sulfur Oxides •
administration & dosage • drug effects* •
etiology* • methods • pathology •
toxicity*},
Abstract = {In this carbonyl sulfide (COS) study, magnetic resonance
microscopy (MRM) and detailed light microscopic evaluation
effectively functioned in parallel to assure that the
distribution and degree of pathology in the brain was
accurately represented. MRM is a powerful imaging modality
that allows for excellent identification of neuroanatomical
structures coupled with the ability to acquire 200 or more
cross-sectional images of the brain, and the ability to
display them in multiple planes. F344 rats were exposed to
200-600 ppm COS for up to 12 weeks. Prior to MRM, rats were
anesthetized and cardiac perfused with McDowell Trump's
fixative containing a gadolinium MR contrast medium. Fixed
specimens were scanned at the Duke Center for In Vivo
Microscopy on a 9.4 Tesla magnetic resonance system adapted
explicitly for microscopic imaging. An advantage of MRM in
this study was the ability to identify lesions in rats that
appeared clinically normal prior to sacrifice and the
opportunity to identify lesions in areas of the brain which
would not be included in conventional studies. Other
advantages include the ability to examine the brain in
multiple planes (transverse, dorsal, sagittal) and obtain
and save the MRM images in a digital format that allows for
postexperimental data processing and manipulation. MRM
images were correlated with neuroanatomical and
neuropathological findings. All suspected MRM images were
compared to corresponding H&E slides. An important aspect of
this study was that MRM was critical in defining our
strategy for sectioning the brain, and for designing
mechanistic studies (cytochrome oxidase evaluations) and
functional assessments (electrophysiology studies) on
specifically targeted anatomical sites following COS
exposure.},
Key = {fds132895}
}
@booklet{Sills04a,
Author = {Sills, RC and Morgan, DL and Maronpot, RR and Johnson,
GA},
Title = {Contribution of magnetic resonance microscopy in the
biologic and mechanistic assessment of carbonyl sulfide
neurotoxicity in F344 rats},
Journal = {TOXICOLOGY AND APPLIED PHARMACOLOGY},
Volume = {197},
Number = {3},
Pages = {151-152},
Publisher = {ACADEMIC PRESS INC ELSEVIER SCIENCE},
Year = {2004},
Month = {June},
ISSN = {0041-008X},
url = {http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000222348900052&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=47d3190e77e5a3a53558812f597b0b92},
Key = {Sills04a}
}
@booklet{Shattuck97a,
Author = {Shattuck, MD and Behringer, RP and Johnson, GA and Georgiadis,
JG},
Title = {Convection and flow in porous media. Part 1. Visualization
by magnetic resonance imaging},
Journal = {Journal of Fluid Mechanics},
Volume = {332},
Pages = {215-245},
Publisher = {Cambridge University Press (CUP)},
Year = {1997},
Month = {February},
url = {http://dx.doi.org/10.1017/s0022112096003990},
Abstract = {We describe an experimental study of porous media convection
(PMC) from onset to 8Rac. The goal of this work is to
provide non-invasive imaging and high-precision heat
transport measurements to test theories of convection in
PMC. We obtain velocity information and visualize the
convection patterns using magnetic resonance imaging (MRI).
We study both ordered and disordered packings of
mono-disperse spheres of diameter d = 3.204 ± 0.029 mm, in
circular, rectangular, and hexagonal planforms. In general,
the structure of the medium plays a role which is not
predicted by theories which assume a homogeneous system.
Disordered media are prepared by pouring mono-disperse
spheres into the container. Large ordered regions of close
packing for the spheres, with grain boundaries and isolated
defects, characterize these media. The defects and grain
boundaries play an important role in pattern formation in
disordered media. Any deviation from close packing produces
a region of larger porosity, hence locally larger
permeability. The result is spatial variations in the
Rayleigh number, Ra. We define the critical Ra, Rac, as the
Rayleigh number at the onset of convection in the ordered
regions. We find that stable localized convective regions
exist around grain boundaries and defects at Ra < Rac. These
remain as pinning sites for the convection patterns in the
ordered regions as Ra increases above Rac up to 5Rac, the
highest Ra studied in the disordered media. In ordered
media, spheres are packed such that the only deviations from
close packing occur within a thin (<d) region near the
vertical walls. Stable localized convection begins at 0.5Rac
in the wall regions but appears to play only a weak role in
the pattern formation of the interior regions (bulk), since
different stable patterns are observed in the bulk at the
same Ra after each cycling of Ra below Rac, even for similar
patterns of small rolls in the wall regions. The experiments
provide a test of the following predictions for PMC: (i)
that straight parallel rolls should be linearly stable for
Rac < Ra < 5Rac; (ii) that at onset, the rolls should have a
dimensionless wavevector qc = π; (iii) that at the upper
end of this range rolls should lose stability to
cross-rolls; (iv) that the initial slope of the Nusselt
curve should be 2; (v) that there should be a rapid decay of
vertical vorticity - hence no complex flows, such as those
which occur for Rayleigh-Bénard convection (RBC) within the
nominal regime of stable parallel rolls. These predictions
are in partial agreement with our findings for the bulk
convection in the ordered media. We observe roll-like
structures which relax rapidly to stable patterns between
Rac and 5Rac. However we find a wavenumber which is 0.7π
compared to π derived from linear stability theory. We find
an asymmetry between the size of the upflowing regions and
downflowing regions as Ra grows above Rac. The ratio of the
volume of the upflowing to the volume of the downflowing
regions decreases as Ra increases and leads to a novel
time-dependent state, which does not consist of cross-rolls.
This time-dependent state begins at 6Rac and is observed up
to 8Rac, the largest Ra which we studied. It seems likely
that the occurrence of this state is linked to departures
from the Boussinesq approximation at higher Ra. We also find
that the slope of the Nusselt curve is 0.7, which does not
agree with the predicted value of 2.},
Doi = {10.1017/s0022112096003990},
Key = {Shattuck97a}
}
@article{fds174234,
Author = {S Banerjee and GA Johnson},
Title = {Coregionalized single- and multiresolution spatially varying
growth curve modeling with application to weed
growth.},
Journal = {Biometrics},
Volume = {62},
Number = {3},
Pages = {864-76},
Year = {2006},
Month = {September},
ISSN = {0006-341X},
url = {http://dx.doi.org/10.1111/j.1541-0420.2006.00535.x},
Keywords = {Bayes Theorem • Biometry • Markov Chains •
Models, Biological* • Models, Statistical* • Monte
Carlo Method • Normal Distribution • Setaria Plant
• growth & development*},
Abstract = {Modeling of longitudinal data from agricultural experiments
using growth curves helps understand conditions conducive or
unconducive to crop growth. Recent advances in Geographical
Information Systems (GIS) now allow geocoding of
agricultural data that help understand spatial patterns. A
particularly common problem is capturing spatial variation
in growth patterns over the entire experimental domain.
Statistical modeling in these settings can be challenging
because agricultural designs are often spatially replicated,
with arrays of subplots, and interest lies in capturing
spatial variation at possibly different resolutions. In this
article, we develop a framework for modeling spatially
varying growth curves as Gaussian processes that capture
associations at single and multiple resolutions. We provide
Bayesian hierarchical models for this setting, where
flexible parameterization enables spatial estimation and
prediction of growth curves. We illustrate using data from
weed growth experiments conducted in Waseca, Minnesota, that
recorded growth of the weed Setaria spp. in a spatially
replicated design.},
Language = {eng},
Doi = {10.1111/j.1541-0420.2006.00535.x},
Key = {fds174234}
}
@article{fds372233,
Author = {G.A. Johnson},
Title = {Correction for Johnson et al., Merged magnetic resonance and
light sheet microscopy of the whole mouse
brain.},
Journal = {Proc Natl Acad Sci U S A},
Volume = {120},
Number = {25},
Pages = {e2308718120},
Year = {2023},
Month = {June},
url = {http://dx.doi.org/10.1073/pnas.2308718120},
Doi = {10.1073/pnas.2308718120},
Key = {fds372233}
}
@booklet{Monk94,
Author = {B. J. Monk and J. A. Chapman and G. A. Johnson and B. K.
Brightman and S. P. Wilczynski and M. J. Schell and H.
Fan},
Title = {Correlation of c-myc and her-2/neu amplification and
expression with histopathologic variables in uterine corpus
cancer},
Journal = {American Journal Of Obstetrics And Gynecology},
Volume = {171},
Number = {5},
Pages = {1193 -- 1198},
Year = {1994},
Month = {November},
Key = {Monk94}
}
@article{fds174109,
Author = {BJ Monk and JA Chapman and GA Johnson and BK Brightman and SP
Wilczynski, MJ Schell and H Fan},
Title = {Correlation of C-myc and HER-2/neu amplification and
expression with histopathologic variables in uterine corpus
cancer.},
Journal = {American journal of obstetrics and gynecology},
Volume = {171},
Number = {5},
Pages = {1193-8},
Year = {1994},
Month = {November},
ISSN = {0002-9378},
Keywords = {Adenocarcinoma • Aged • Autoradiography •
Blotting, Northern • Blotting, Southern • Female
• Gene Amplification* • Gene Expression* •
Genes, erbB-2* • Genes, myc* • Humans •
Middle Aged • Uterine Neoplasms • genetics* •
pathology • pathology*},
Abstract = {OBJECTIVE: Initial studies of protooncogenes in uterine
corpus cancer have focused on a single aspect of the gene in
question (deoxyribonucleic acid, ribonucleic acid, protein)
or have studied a small number of patients. Therefore we
evaluated c-myc and HER-2/neu gene amplification and
ribonucleic acid overexpression in such malignancies and
correlated these molecular changes with known pathologic
risk factors. STUDY DESIGN: Quantitative Southern blot
analysis for oncogene deoxyribonucleic acid was used to
examine 37 tumors from patients with primary untreated
uterine corpus cancer referred to the City of Hope National
Medical Center. Six normal endometrial specimens were
controls. Seventeen tumors were also examined by Northern
blotting to assess increased ribonucleic expression.
RESULTS: Histologic types included adenocarcinoma (n = 30),
papillary serous adenocarcinoma (n = 2), adenosquamous
carcinoma (n = 2), mixed mullerian sarcoma (n = 2), and
leiomyosarcoma (n = 1). Carcinomas were stage I (n = 10), II
(n = 18), or III (n = 6). Twenty-three had myometrial
invasion of less than one third, six one third to two
thirds, and eight deeper invasion (greater than two thirds).
According to the criteria of the International Federation of
Gynecology and Obstetrics stage was as follows: I (n = 22),
II (n = 3), III (n = 7), and IV (n = 5). Ten (27%) and four
(11%) tumors showed gene amplification of c-myc and
HER-2/neu, respectively. Six demonstrated overexpression of
either the c-myc or HER-2/neu gene. HER-2/neu gene
amplification was associated more closely with
overexpression. Stepwise logistic analysis demonstrated
c-myc amplification to be associated with higher grade (p =
0.01). CONCLUSION: In this referral population, c-myc
activation is more common than HER-2/neu activation in
uterine corpus cancer and is associated with tumors of
higher grade.},
Language = {eng},
Key = {fds174109}
}
@booklet{Scribner99,
Author = {D. R. Scribner and R. S. Mannel and J. L. Walker and G. A.
Johnson},
Title = {Cost analysis of laparoscopy versus laparotomy for early
endometrial cancer},
Journal = {Gynecologic Oncology},
Volume = {75},
Number = {3},
Pages = {460 -- 463},
Year = {1999},
Month = {December},
Key = {Scribner99}
}
@article{fds174313,
Author = {DR Scribner Jr and RS Mannel and JL Walker and GA
Johnson},
Title = {Cost analysis of laparoscopy versus laparotomy for early
endometrial cancer.},
Journal = {Gynecologic oncology},
Volume = {75},
Number = {3},
Pages = {460-3},
Year = {1999},
Month = {December},
ISSN = {0090-8258},
url = {http://dx.doi.org/10.1006/gyno.1999.5606},
Keywords = {Aged • Costs and Cost Analysis • Endometrial
Neoplasms • Female • Humans • Laparoscopy
• Laparotomy • Lymph Node Excision • Neoplasm
Staging • Retrospective Studies • economics*
• pathology • surgery*},
Abstract = {OBJECTIVE: The purpose of this study was to determine
whether the cost associated with treatment of early stage
endometrial cancer differs on the basis of the surgical
approach. METHODS: A retrospective analysis was performed on
a series of women with presumed early stage endometrial
cancer treated between 5/96 and 1/99 at a single
institution. The patients were grouped according to the
surgical approach utilized. The first group consisted of 19
patients who underwent laparoscopic assisted vaginal
hysterectomy, bilateral salpingo-oophorectomy, and
laparoscopic pelvic and paraaortic lymph node dissection.
The second group consisted of 17 patients who underwent a
total abdominal hysterectomy, bilateral salpingo-oophorectomy,
and pelvic and paraaortic lymph node dissection. The two
groups were compared with a two-tailed Student t test.
Variables analyzed included age, Quetelet index (QI),
surgical stage, number of lymph nodes, surgical time,
estimated blood loss, postoperative complications, number of
days in the hospital, and costs. The cost analysis was
divided into room and board, pharmacy, ancillary services,
operating room equipment, operating room services, and
anesthesia. RESULTS: Both groups were similar in age, QI,
and distribution of stage. The laparoscopic group required
more OR time (237 vs 157 min, P < 0.001); however, the
number of lymph nodes, estimated blood loss, and
postoperative complications were not significantly different
between the groups. The laparoscopic group required
significantly shorter hospitalization than the laparotomy
group (3.7 vs 5.2 days, P < 0.001) resulting in less room
and board ($299 vs $454, P < 0.001) as well as pharmacy
costs ($443 vs $625, P < 0.02). The cost of anesthesia was
higher in the laparoscopic group ($696 vs $444, P < 0.001)
but the costs of OR equipment, OR services, and total costs
were not statistically different between the groups.
CONCLUSION: Laparoscopic surgical management of early stage
endometrial cancer is feasible with minimal morbidity. The
cost savings of early hospital discharge is offset by longer
surgical time and higher anesthetic costs. The total costs
for each surgical approach are not statistically different.
The presumed advantages of less pain, early resumption of
normal activities, and overall improvement of quality of
life await further investigation.},
Language = {eng},
Doi = {10.1006/gyno.1999.5606},
Key = {fds174313}
}
@booklet{Johnson93a,
Author = {G. A. Johnson and M. S. Defelice and Z. R.
Helsel},
Title = {Cover crop management and weed-control in corn
(zea-mays)},
Journal = {Weed Technology},
Volume = {7},
Number = {2},
Pages = {425 -- 430},
Year = {1993},
Key = {Johnson93a}
}
@article{fds174145,
Author = {GA Johnson and SJ Boukma and RA Lahti and J Mathews},
Title = {Cyclic AMP and phosphodiesterase in synaptic vesicles from
mouse brain.},
Journal = {Journal of neurochemistry},
Volume = {20},
Number = {5},
Pages = {1387-92},
Year = {1973},
Month = {May},
ISSN = {0022-3042},
Keywords = {Animals • Brain • Brain Chemistry* • Cattle
• Cell Fractionation • Centrifugation, Density
Gradient • Cyclic AMP • Male • Mice •
Microscopy, Electron • Myocardium • Phosphoric
Diester Hydrolases • Synaptic Vesicles • Tritium
• analysis* • cytology • enzymology},
Language = {eng},
Key = {fds174145}
}
@article{fds349540,
Author = {Wang, N and White, LE and Qi, Y and Cofer, G and Johnson,
GA},
Title = {Cytoarchitecture of the mouse brain by high resolution
diffusion magnetic resonance imaging.},
Journal = {Neuroimage},
Volume = {216},
Pages = {116876},
Year = {2020},
Month = {August},
url = {http://dx.doi.org/10.1016/j.neuroimage.2020.116876},
Abstract = {MRI has been widely used to probe the neuroanatomy of the
mouse brain, directly correlating MRI findings to histology
is still challenging due to the limited spatial resolution
and various image contrasts derived from water relaxation or
diffusion properties. Magnetic resonance histology has the
potential to become an indispensable research tool to
mitigate such challenges. In the present study, we acquired
high spatial resolution MRI datasets, including diffusion
MRI (dMRI) at 25 μm isotropic resolution and
quantitative susceptibility mapping (QSM) at 21.5 μm
isotropic resolution to validate with conventional mouse
brain histology. Diffusion weighted images (DWIs) show
better delineation of cortical layers and glomeruli in the
olfactory bulb than fractional anisotropy (FA) maps.
However, among all the image contrasts, including
quantitative susceptibility mapping (QSM), T1/T2∗ images
and DTI metrics, FA maps highlight unique laminar
architecture in sub-regions of the hippocampus, including
the strata of the dentate gyrus and CA fields of the
hippocampus. The mean diffusivity (MD) and axial diffusivity
(AD) yield higher correlation with DAPI (0.62 and 0.71) and
NeuN (0.78 and 0.74) than with NF-160 (-0.34 and -0.49). The
correlations between FA and DAPI, NeuN, and NF-160 are 0.31,
-0.01, and -0.49, respectively. Our findings demonstrate
that MRI at microscopic resolution deliver a
three-dimensional, non-invasive and non-destructive platform
for characterization of fine structural detail in both gray
matter and white matter of the mouse brain.},
Doi = {10.1016/j.neuroimage.2020.116876},
Key = {fds349540}
}
@article{fds174238,
Author = {GA Johnson and SM Jalal},
Title = {DDT-induced chromosomal damage in mice.},
Journal = {The Journal of heredity},
Volume = {64},
Number = {1},
Pages = {7-8},
Year = {1973},
Month = {June},
ISSN = {0022-1503},
Keywords = {Animals • Chromatids • Chromosome Aberrations*
• Chromosomes • DDT • Mice • Mice,
Inbred BALB C • Mutagens • drug effects •
drug effects* • pharmacology*},
Language = {eng},
Key = {fds174238}
}
@article{fds174292,
Author = {LE Steadman and GA Johnson and EL Belden and WJ Murdoch},
Title = {Decline in sialic acid composition of cellular membranes
isolated from ovine corpora lutea during
prostaglandin-induced luteolysis: apparent independence of
autoimmune recognition.},
Journal = {American journal of reproductive immunology (New York, N.Y.
: 1989)},
Volume = {19},
Number = {1},
Pages = {1-2},
Year = {1989},
Month = {January},
ISSN = {1046-7408},
Keywords = {Animals • Autoantibodies • Cell Membrane •
Corpus Luteum • Dinoprost • Female •
N-Acetylneuraminic Acid • Sheep • Sialic Acids
• analysis • analysis* • drug effects •
pharmacology* • physiology*},
Abstract = {Sialic acid was quantified in plasma membranes of corpora
lutea isolated during prostaglandin (PG) F2 alpha-induced
luteolysis in sheep. Concentrations of sialic acid within
membranes decreased after injection of PGF2 alpha, and
before signs of luteal regression (i.e., a decline in tissue
concentrations of progesterone) were manifested. Removal of
residues of sialic acid from luteal membranes was not
associated with cellular binding of gamma globulin, as
monitored by indirect immunofluorescence microscopy. We
suggest that desialylation of luteal membranes could be an
important aspect of the mechanism of luteolysis. Such a
process does not appear to involve participation of
autoantibody.},
Language = {eng},
Key = {fds174292}
}
@booklet{Steadman89,
Author = {L. E. Steadman and G. A. Johnson and E. L. Belden and W. J.
Murdoch},
Title = {Decline in sialic-acid composition of cellular membranes
isolated from ovine corpora-lutea during
prostaglandin-induced luteolysis - apparent independence of
autoimmune recognition},
Journal = {American Journal Of Reproductive Immunology},
Volume = {19},
Number = {1},
Pages = {1 -- 2},
Year = {1989},
Month = {January},
Key = {Steadman89}
}
@article{fds132725,
Author = {G.A. Johnson},
Title = {Delnomdedieu M, Hedlund LW, maronpot RR, Johson GA, MR
microscopy and histopathology: Comparative approach to
bromobenzene-induced hepatoxicity in the rat. hepatology 27;
526-532 1998},
Year = {1998},
Key = {fds132725}
}
@article{fds268788,
Author = {Clark, D and Johnson, GA and Badea, CT},
Title = {Denoising of 4D Cardiac Micro-CT Data Using Median-Centric
Bilateral Filtration.},
Journal = {Proc SPIE Int Soc Opt Eng},
Volume = {8314},
Year = {2012},
Month = {February},
ISSN = {1605-7422},
url = {http://dx.doi.org/10.1117/12.911478},
Abstract = {Bilateral filtration has proven an effective tool for
denoising CT data. The classic filter utilizes Gaussian
domain and range weighting functions in 2D. More recently,
other distributions have yielded more accurate results in
specific applications, and the bilateral filtration
framework has been extended to higher dimensions. In this
study, brute-force optimization is employed to evaluate the
use of several alternative distributions for both domain and
range weighting: Andrew's Sine Wave, El Fallah Ford,
Gaussian, Flat, Lorentzian, Huber's Minimax, Tukey's
Bi-weight, and Cosine. Two variations on the classic
bilateral filter which use median filtration to reduce bias
in range weights are also investigated: median-centric and
hybrid bilateral filtration. Using the 4D MOBY mouse phantom
reconstructed with noise (stdev. ~ 65 HU), hybrid bilateral
filtration, a combination of the classic and median-centric
filters, with Flat domain and range weighting is shown to
provide optimal denoising results (PSNRs: 31.69, classic;
31.58 median-centric; 32.25, hybrid). To validate these
phantom studies, the optimal filters are also applied to in
vivo, 4D cardiac micro-CT data acquired in the mouse. In a
constant region of the left ventricle, hybrid bilateral
filtration with Flat domain and range weighting is shown to
provide optimal smoothing (stdev: original, 72.2 HU;
classic, 20.3 HU; median-centric, 24.1 HU; hybrid, 15.9 HU).
While the optimal results were obtained using 4D filtration,
the 3D hybrid filter is ultimately recommended for denoising
4D cardiac micro-CT data because it is more computationally
tractable and less prone to artifacts (MOBY PSNR: 32.05;
left ventricle stdev: 20.5 HU).},
Doi = {10.1117/12.911478},
Key = {fds268788}
}
@booklet{Todd01a,
Author = {M. D. Todd and G. A. Johnson and S. T. Vohra},
Title = {Depolyment of a fiber Bragg grating-based measurement system
in a structural health monitoring application},
Journal = {Smart Materials \& Structures},
Volume = {10},
Number = {3},
Pages = {534 -- 539},
Year = {2001},
Month = {June},
Key = {Todd01a}
}
@booklet{Hayes84,
Author = {HAYES, CE and JOHNSON, GA and HERFKENS, RJ},
Title = {DESIGN CONSIDERATIONS FOR SMALL ANIMAL IMAGING IN A LARGE
BORE MRI SYSTEM},
Journal = {INVESTIGATIVE RADIOLOGY},
Volume = {19},
Number = {5},
Pages = {S25-S25},
Publisher = {LIPPINCOTT-RAVEN PUBL},
Year = {1984},
Month = {January},
ISSN = {0020-9996},
url = {http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:A1984TL42800118&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=47d3190e77e5a3a53558812f597b0b92},
Key = {Hayes84}
}
@article{fds268848,
Author = {Nouls, JC and Izenson, MG and Greeley, HP and Johnson,
GA},
Title = {Design of a superconducting volume coil for magnetic
resonance microscopy of the mouse brain.},
Journal = {J Magn Reson},
Volume = {191},
Number = {2},
Pages = {231-238},
Year = {2008},
Month = {April},
ISSN = {1090-7807},
url = {http://www.ncbi.nlm.nih.gov/pubmed/18221901},
Keywords = {Animals • Brain • Electric Conductivity •
Equipment Design • Equipment Failure Analysis •
Magnetic Resonance Imaging • Magnetics • Mice
• Microscopy • Transducers • cytology* •
instrumentation* • methods* • veterinary •
veterinary*},
Abstract = {We present the design process of a superconducting volume
coil for magnetic resonance microscopy of the mouse brain at
9.4T. The yttrium barium copper oxide coil has been designed
through an iterative process of three-dimensional
finite-element simulations and validation against room
temperature copper coils. Compared to previous designs, the
Helmholtz pair provides substantially higher B(1)
homogeneity over an extended volume of interest sufficiently
large to image biologically relevant specimens. A
custom-built cryogenic cooling system maintains the
superconducting probe at 60+/-0.1K. Specimen loading and
probe retuning can be carried out interactively with the
coil at operating temperature, enabling much higher
through-put. The operation of the probe is a routine,
consistent procedure. Signal-to-noise ratio in a mouse brain
increased by a factor ranging from 1.1 to 2.9 as compared to
a room-temperature solenoid coil optimized for mouse brain
microscopy. We demonstrate images encoded at 10x10x20mum for
an entire mouse brain specimen with signal-to-noise ratio of
18 and a total acquisition time of 16.5h, revealing
neuroanatomy unseen at lower resolution. Phantom
measurements show an effective spatial resolution better
than 20mum.},
Doi = {10.1016/j.jmr.2007.12.018},
Key = {fds268848}
}
@article{fds132860,
Author = {X Zhou and RR Maronpot and LW Hedlund and GP Cofer and GA
Johnson},
Title = {Detection of bromobenzene-induced hepatocellular necrosis
using magnetic resonance microscopy.},
Journal = {Magnetic resonance in medicine : official journal of the
Society of Magnetic Resonance in Medicine / Society of
Magnetic Resonance in Medicine, UNITED STATES},
Volume = {34},
Number = {6},
Pages = {853-7},
Year = {1995},
Month = {December},
ISSN = {0740-3194},
Keywords = {Animals • Bromobenzenes • Dose-Response
Relationship, Drug • Female • Image Processing,
Computer-Assisted • Liver • Magnetic Resonance
Spectroscopy • Necrosis • Rats • Rats, Inbred
F344 • Sensitivity and Specificity • diagnostic
use* • drug effects* • methods • pathology
• toxicity*},
Abstract = {The authors used magnetic resonance (MR) microscopy to
assess hepatic tissue damage induced by bromobenzene both in
living rats and in fixed rat liver tissues. Experiments were
conducted at 7 Tesla on three groups of Fisher rats treated
with bromobenzene at a single dose of 68, 135, and 269
mg/kg, respectively. Optical microscopy of hematoxylin and
eosin stained sections showed liver damage only at the
highest dose, whereas with MR microscopy, tissue alterations
were detected at all three doses both in vivo and ex vivo.
The contrast mechanism of the superior sensitivity of MR
microscopy is believed to be related to the changes in local
diffusion coefficients that accompany cellular degeneration
and death, although other contrast mechanisms may also be
involved. The superior sensitivity of MR microscopy, as
demonstrated in this study, has many implications for
potential use of MR techniques to perform in vivo
histology.},
Key = {fds132860}
}
@booklet{Zhou95,
Author = {Zhou, X and Maronpot, RR and Hedlund, LW and Cofer, GP and Johnson,
GA},
Title = {Detection of bromobenzene-induced hepatocellular necrosis
using magnetic resonance microscopy.},
Journal = {Magn Reson Med},
Volume = {34},
Number = {6},
Pages = {853-857},
Year = {1995},
Month = {December},
ISSN = {0740-3194},
url = {http://www.ncbi.nlm.nih.gov/pubmed/8598812},
Abstract = {The authors used magnetic resonance (MR) microscopy to
assess hepatic tissue damage induced by bromobenzene both in
living rats and in fixed rat liver tissues. Experiments were
conducted at 7 Tesla on three groups of Fisher rats treated
with bromobenzene at a single dose of 68, 135, and 269
mg/kg, respectively. Optical microscopy of hematoxylin and
eosin stained sections showed liver damage only at the
highest dose, whereas with MR microscopy, tissue alterations
were detected at all three doses both in vivo and ex vivo.
The contrast mechanism of the superior sensitivity of MR
microscopy is believed to be related to the changes in local
diffusion coefficients that accompany cellular degeneration
and death, although other contrast mechanisms may also be
involved. The superior sensitivity of MR microscopy, as
demonstrated in this study, has many implications for
potential use of MR techniques to perform in vivo
histology.},
Doi = {10.1002/mrm.1910340610},
Key = {Zhou95}
}
@article{fds269111,
Author = {Chen, XJ and Hedlund, LW and Möller, HE and Chawla, MS and Maronpot,
RR and Johnson, GA},
Title = {Detection of emphysema in rat lungs by using magnetic
resonance measurements of 3He diffusion.},
Journal = {Proc Natl Acad Sci U S A},
Volume = {97},
Number = {21},
Pages = {11478-11481},
Year = {2000},
Month = {October},
ISSN = {0027-8424},
url = {http://www.ncbi.nlm.nih.gov/pubmed/11027348},
Keywords = {Animals • Emphysema • Helium • Humans •
Lung • Magnetic Resonance Imaging • Male •
Rats • Rats, Inbred F344 • diagnosis* •
pathology*},
Abstract = {Emphysema is a pulmonary disease characterized by alveolar
wall destruction, resulting in enlargement of gas exchange
spaces without fibrosis. This condition is a part of chronic
obstructive pulmonary disease (COPD), which causes 3.5% of
deaths worldwide [Anonymous (1990) World Health Stat. Q.
Special, 1-51] and contributes greatly to the global burden
of disease [Murray, C. J. & Lopez, A. D. (1996) Science 274,
740-743]. Alveolar regeneration has been shown in animal
models and could have potential for clinical treatment of
early-stage emphysema. However, current techniques for
detection of emphysema are not sensitive at the initial
stages. Early-stage human panacinar emphysema is modeled in
elastase-treated animals. Here, we provide an in vivo
imaging method for differentiating normal and emphysematous
rat lungs by measuring the apparent diffusion coefficient
(ADC) of hyperpolarized (3)He by using magnetic resonance
imaging. These data show that the ADC is significantly
larger in elastase-treated rats, indicating alveolar
expansion. Whereas these rats were clinically asymptomatic,
conventional histology confirmed presence of injury. Our
results indicate that measurement of the hyperpolarized
(3)He ADC can be a valuable research tool and has potential
application in the clinical setting.},
Doi = {10.1073/pnas.97.21.11478},
Key = {fds269111}
}
@booklet{Chen00,
Author = {X. J. Chen and L. W. Hedlund and H. E. Moller and M. S.
Chawla and R. R. Maronpot and G. A. Johnson},
Title = {Detection of emphysema in rat lungs by using magnetic
resonance measurements of He-3 diffusion},
Journal = {Proceedings Of The National Academy Of Sciences Of The
United States Of America},
Volume = {97},
Number = {21},
Pages = {11478 -- 11481},
Year = {2000},
Month = {October},
Key = {Chen00}
}
@article{fds268798,
Author = {Smith, WM and Hsu, JCM and Johnson, GA and Reimer, KA and Ideker,
RE},
Title = {Detection of infarcted tissue the heart by magnetic
resonance imaging},
Journal = {Proceedings of the Annual Conference on Engineering in
Medicine and Biology},
Volume = {15},
Number = {pt 1},
Pages = {485-486},
Year = {1993},
Month = {December},
Abstract = {Morphological analysis of myocardial infarct structure has
demonstrated that there is probably an anatomical basis for
the induction and maintenance of ventricular tachycardia.
The further study of this phenomenon requires an efficient,
accurate method for quantitative analysis of pathological
anatomy. We have evaluated the ability of magnetic resonance
imaging to distinguish between normal and infarcted tissue
in formalin-fixed human hearts with distant myocardial
infarctions.},
Key = {fds268798}
}
@article{fds268897,
Author = {Benveniste, H and Einstein, G and Kim, KR and Hulette, C and Johnson,
GA},
Title = {Detection of neuritic plaques in Alzheimer's disease by
magnetic resonance microscopy.},
Journal = {Proc Natl Acad Sci U S A},
Volume = {96},
Number = {24},
Pages = {14079-14084},
Year = {1999},
Month = {November},
ISSN = {0027-8424},
url = {http://www.ncbi.nlm.nih.gov/pubmed/10570201},
Keywords = {Aged • Aged, 80 and over • Alzheimer Disease
• Coloring Agents • Humans • Magnetic
Resonance Imaging • Middle Aged • Protons •
Senile Plaques • methods • pathology*},
Abstract = {Magnetic resonance microscopy (MRM) theoretically provides
the spatial resolution and signal-to-noise ratio needed to
resolve neuritic plaques, the neuropathological hallmark of
Alzheimer's disease (AD). Two previously unexplored MR
contrast parameters, T2* and diffusion, are tested for
plaque-specific contrast to noise. Autopsy specimens from
nondemented controls (n = 3) and patients with AD (n = 5)
were used. Three-dimensional T2* and diffusion MR images
with voxel sizes ranging from 3 x 10(-3) mm(3) to 5.9 x
10(-5) mm(3) were acquired. After imaging, specimens were
cut and stained with a microwave king silver stain to
demonstrate neuritic plaques. From controls, the alveus,
fimbria, pyramidal cell layer, hippocampal sulcus, and
granule cell layer were detected by either T2* or diffusion
contrast. These structures were used as landmarks when
correlating MRMs with histological sections. At a voxel
resolution of 5.9 x 10(-5) mm(3), neuritic plaques could be
detected by T2*. The neuritic plaques emerged as black,
spherical elements on T2* MRMs and could be distinguished
from vessels only in cross-section when presented in three
dimension. Here we provide MR images of neuritic plaques in
vitro. The MRM results reported provide a new direction for
applying this technology in vivo. Clearly, the ability to
detect and follow the early progression of amyloid-positive
brain lesions will greatly aid and simplify the many
possibilities to intervene pharmacologically in
AD.},
Doi = {10.1073/pnas.96.24.14079},
Key = {fds268897}
}
@booklet{Anderson93a,
Author = {C. S. Anderson and K. D. Jamrozik and P. W. Burvill and T.
M. H. Chakera and G. A. Johnson and E. G.
Stewartwynne},
Title = {Determining the incidence of different subtypes of stroke -
results from the perth community stroke study,
1989-1990},
Journal = {Medical Journal Of Australia},
Volume = {158},
Number = {2},
Pages = {85 -- 89},
Year = {1993},
Month = {January},
Key = {Anderson93a}
}
@article{fds174307,
Author = {CS Anderson and KD Jamrozik and PW Burvill and TM Chakera and GA
Johnson, EG Stewart-Wynne},
Title = {Determining the incidence of different subtypes of stroke:
results from the Perth Community Stroke Study,
1989-1990.},
Journal = {The Medical journal of Australia},
Volume = {158},
Number = {2},
Pages = {85-9},
Year = {1993},
Month = {January},
ISSN = {0025-729X},
Keywords = {Adult • Aged • Aged, 80 and over • Cerebral
Hemorrhage • Cerebral Infarction • Cerebrovascular
Disorders • Female • Humans • Intracranial
Embolism and Thrombosis • Male • Middle Aged
• Western Australia • classification* •
epidemiology • epidemiology*},
Abstract = {OBJECTIVE: To determine the incidence and case fatality of
seven distinct subtypes of stroke in Perth, Western
Australia. DESIGN AND SETTING: A population-based
descriptive epidemiological study. SUBJECTS: All residents
of a geographically defined segment of the Perth
metropolitan area (estimated population 138,708 persons) who
had a stroke or transient ischaemic attack between 20
February 1989 and 19 August 1990, inclusive. MAIN OUTCOME
MEASURES: The following subtypes of stroke were classified
according to standard clinical, radiological and
pathological criteria: types of cerebral infarction, namely,
large artery (thrombotic) occlusive infarction (LAOI),
cerebral embolic infarction (EMBI), lacunar infarction
(LACI) and boundary zone infarction (BZI); primary
intracerebral haemorrhage (PICH); subarachnoid haemorrhage
(SAH); and stroke of undetermined cause. RESULTS: Over the
18-month study period 536 stroke events were registered, of
which 86% (95% confidence interval, 83%-89%) had a defined
"pathological" diagnosis on the basis of computed
tomographic scanning, magnetic resonance imaging or
necropsy. Cerebral infarction accounted for 71% of cases
(95% CI, 68%-75%), PICH 11% (95% CI, 9%-14%) and SAH 4% (95%
CI, 2%-5%). The 382 cases of cerebral infarction included
LAOI (in approximately 71%), EMBI (15%), LACI (10%) and BZI
(5%). While the incidence of all subtypes of stroke
increased with age, there were age and sex differences in
their proportional frequency, management and prognosis:
patients with PICH, SAH and EMBI were more likely to be
admitted to hospital, and these conditions carried the
highest early case fatality. Over all, the 28-day case
fatality was 24% (95% CI, 20%-28%), but varied from 0 for
LACI and BZI, to 37% (95% CI, 15%-59%) for SAH and 35% (CI,
23%-47%) for PICH. CONCLUSIONS: In this study, we found
considerable differences in incidence rates, the effect of
age and sex on incidence rates, and prognosis for the
different subtypes of stroke. Hospital-based studies are
likely to be selectively biased by emphasising strokes that
are severe and require admission to hospital. These data
have important implications in the design and evaluation of
clinical trials of therapy for stroke.},
Language = {eng},
Key = {fds174307}
}
@booklet{Johnson99a,
Author = {G. A. Johnson and R. C. Burghardt and G. R. Newton and F. W.
Bazer and T. E. Spencer},
Title = {Development and characterization of immortalized ovine
endometrial cell lines},
Journal = {Biology Of Reproduction},
Volume = {61},
Number = {5},
Pages = {1324 -- 1330},
Year = {1999},
Month = {November},
Key = {Johnson99a}
}
@article{fds174198,
Author = {GA Johnson and RC Burghardt and GR Newton and FW Bazer and TE
Spencer},
Title = {Development and characterization of immortalized ovine
endometrial cell lines.},
Journal = {Biology of reproduction},
Volume = {61},
Number = {5},
Pages = {1324-30},
Year = {1999},
Month = {November},
ISSN = {0006-3363},
Keywords = {Animals • Blotting, Western • Cell Line •
Cell Nucleus • DNA-Binding Proteins • Endocrine
System • Endometrium • Female • Fluorescent
Antibody Technique, Direct • Interferon Regulatory
Factor-1 • Interferon Type I • Microscopy,
Fluorescence • Paracrine Communication •
Phosphoproteins • Pregnancy Proteins • Receptors,
Estrogen • Receptors, Progesterone • Recombinant
Proteins • Sheep • Signal Transduction •
Transcription Factors • Ubiquitins • analogs &
derivatives • biosynthesis • cytology* •
metabolism • physiology},
Abstract = {The objective of this study was to generate immortalized
endometrial epithelial and stromal cell lines from the ovine
uterus. Luminal (LE) and glandular epithelial (GE) cells and
stromal (ST) cells were enzymatically isolated from the
uterus of a Day 5 cyclic ewe (estrus on Day 0), and primary
cultures were immortalized by transduction with a retroviral
vector (LXSN-16E6E7) packaged by the amphotropic fibroblast
line PA-317. Cells having integrated the vector were
selected by resistance to the neomycin analogue G418
(0.6-0.8 mg/ml). Surviving cells were maintained in complete
culture medium containing G418 (0.1 mg/ml) and subcultured
for more than 40 passages. Phase-contrast microscopy
revealed that LE and GE cells exhibited a cobblestone
morphology whereas immortalized ST cells were spindle
shaped. The epithelial origin of LE and GE was confirmed by
positive cytokeratin immunostaining, and ST cells were
vimentin positive. All cell lines were negative for smooth
muscle alpha-actin staining. Western blot analyses of cell
extracts revealed the presence of signal transducers and
activators of transcription (STAT) proteins 1, 2, and 3. In
the LE cells, interferon tau (IFNtau) induced nuclear
translocation of STAT proteins 1 and 2 and up-regulated
several IFN-inducible genes, including STATs 1, 2, and 3 and
ubiquitin cross-reactive protein (UCRP/ISG17). In the LE
cell line, IFN regulatory factor one was transiently
up-regulated and then down-regulated by IFNtau.
Immunostaining revealed the presence of nuclear estrogen
receptor and progesterone receptor in all cell lines. These
ovine endometrial cell lines provide useful in vitro model
systems for the study of hormone and cytokine action, signal
transduction pathways, cell-cell interactions, and gene
expression in specific cell types of the ovine
endometrium.},
Language = {eng},
Key = {fds174198}
}
@booklet{Spencer99b,
Author = {T. E. Spencer and G. A. Johnson and R. C. Burghardt and J.
A. G. W. Fleming and F. W. Bazer},
Title = {Development and characterization of immortalized ovine
endometrial cell lines.},
Journal = {Biology Of Reproduction},
Volume = {60},
Pages = {142 -- 142},
Year = {1999},
Key = {Spencer99b}
}
@booklet{Stern88,
Author = {STERN, RL and CLINE, HE and JOHNSON, GA and RAVIN,
CE},
Title = {DEVELOPMENT OF A 3D RECONSTRUCTED-IMAGE SURGICAL PLANNING
STATION},
Journal = {INVESTIGATIVE RADIOLOGY},
Volume = {23},
Number = {9},
Pages = {S60-S60},
Publisher = {LIPPINCOTT-RAVEN PUBL},
Year = {1988},
Month = {September},
ISSN = {0020-9996},
url = {http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:A1988Q574500240&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=47d3190e77e5a3a53558812f597b0b92},
Key = {Stern88}
}
@booklet{Segars04,
Author = {Segars, WP and Tsui, BMW and Frey, EC and Johnson, GA and Berr,
SS},
Title = {Development of a 4-D digital mouse phantom for molecular
imaging research.},
Journal = {Mol Imaging Biol},
Volume = {6},
Number = {3},
Pages = {149-159},
Year = {2004},
ISSN = {1536-1632},
url = {http://www.ncbi.nlm.nih.gov/pubmed/15193249},
Abstract = {PURPOSE: We develop a realistic and flexible 4-D digital
mouse phantom and investigate its usefulness in molecular
imaging research. METHODS: Organ shapes were modeled with
non-uniform rational B-spline (NURBS) surfaces based on
high-resolution 3-D magnetic resonance microscopy (MRM)
data. Cardiac and respiratory motions were modeled based on
gated magnetic resonance imaging (MRI) data obtained from
normal mice. Pilot simulation studies in single-photon
emission computed tomography (SPECT) and X-ray computed
tomography (CT) were performed to demonstrate the utility of
the phantom. RESULTS: NURBS are an efficient and flexible
way to accurately model the anatomy and cardiac and
respiratory motions for a realistic 4-D digital mouse
phantom. The phantom is capable of producing realistic
molecular imaging data from which imaging devices and
techniques can be evaluated. CONCLUSION: The phantom
provides a unique and useful tool in molecular imaging
research. It can be used in the development of new imaging
instrumentation, image acquisition strategies, and image
processing and reconstruction methods.},
Doi = {10.1016/j.mibio.2004.03.002},
Key = {Segars04}
}
@article{fds132894,
Author = {WP Segars and BM Tsui and EC Frey and GA Johnson and SS
Berr},
Title = {Development of a 4-D digital mouse phantom for molecular
imaging research.},
Journal = {Molecular imaging and biology : MIB : the official
publication of the Academy of Molecular Imaging, United
States},
Volume = {6},
Number = {3},
Pages = {149-59},
ISSN = {1536-1632},
Keywords = {Animals • Image Processing, Computer-Assisted •
Mice • Models, Anatomic* • Models, Animal* •
Phantoms, Imaging* • Tomography, Emission-Computed,
Single-Photon • Tomography, X-Ray Computed •
anatomy & histology* • instrumentation •
instrumentation* • physiology},
Abstract = {PURPOSE: We develop a realistic and flexible 4-D digital
mouse phantom and investigate its usefulness in molecular
imaging research. METHODS: Organ shapes were modeled with
non-uniform rational B-spline (NURBS) surfaces based on
high-resolution 3-D magnetic resonance microscopy (MRM)
data. Cardiac and respiratory motions were modeled based on
gated magnetic resonance imaging (MRI) data obtained from
normal mice. Pilot simulation studies in single-photon
emission computed tomography (SPECT) and X-ray computed
tomography (CT) were performed to demonstrate the utility of
the phantom. RESULTS: NURBS are an efficient and flexible
way to accurately model the anatomy and cardiac and
respiratory motions for a realistic 4-D digital mouse
phantom. The phantom is capable of producing realistic
molecular imaging data from which imaging devices and
techniques can be evaluated. CONCLUSION: The phantom
provides a unique and useful tool in molecular imaging
research. It can be used in the development of new imaging
instrumentation, image acquisition strategies, and image
processing and reconstruction methods.},
Key = {fds132894}
}
@article{fds268840,
Author = {Zhang, X and Badea, C and Jacob, M and Johnson, GA},
Title = {Development of a noncontact 3-D fluorescence tomography
system for small animal in vivo imaging.},
Journal = {Proc SPIE Int Soc Opt Eng},
Volume = {7191},
Pages = {nihpa106691},
Year = {2009},
Month = {February},
ISSN = {0277-786X},
url = {http://www.ncbi.nlm.nih.gov/pubmed/19587837},
Abstract = {Fluorescence imaging is an important tool for tracking
molecular-targeting probes in preclinical studies. It offers
high sensitivity, but nonetheless low spatial resolution
compared to other leading imaging methods such CT and MRI.
We demonstrate our methodological development in small
animal in vivo whole-body imaging using fluorescence
tomography. We have implemented a noncontact fluid-free
fluorescence diffuse optical tomography system that uses a
raster-scanned continuous-wave diode laser as the light
source and an intensified CCD camera as the photodetector.
The specimen is positioned on a motorized rotation stage.
Laser scanning, data acquisition, and stage rotation are
controlled via LabVIEW applications. The forward problem in
the heterogeneous medium is based on a normalized Born
method, and the sensitivity function is determined using a
Monte Carlo method. The inverse problem (image
reconstruction) is performed using a regularized iterative
algorithm, in which the cost function is defined as a
weighted sum of the L-2 norms of the solution image, the
residual error, and the image gradient. The relative weights
are adjusted by two independent regularization parameters.
Our initial tests of this imaging system were performed with
an imaging phantom that consists of a translucent plastic
cylinder filled with tissue-simulating liquid and two
thin-wall glass tubes containing indocyanine green. The
reconstruction is compared to the output of a finite element
method-based software package NIRFAST and has produced
promising results.},
Doi = {10.1117/12.808199},
Key = {fds268840}
}
@booklet{Ruwart90,
Author = {M. J. Ruwart and S. K. Sharma and D. W. Harris and D. B.
Lakings and B. D. Rush and K. F. Wilkinson and J. C.
Cornette and D. B. Evans and J. M. Friis and K. J. Cook and G. A. Johnson},
Title = {Development of a sensitive activity assay for high-volume
evaluation of human renin inhibitory peptides in rat serum -
results with u-71,038},
Journal = {Pharmaceutical Research},
Volume = {7},
Number = {4},
Pages = {407 -- 410},
Year = {1990},
Month = {April},
Key = {Ruwart90}
}
@article{fds174226,
Author = {M Zeiler and R Leiser and GA Johnson and HR Tinneberg and C
Pfarrer},
Title = {Development of an in vitro model for bovine placentation: a
comparison of the in vivo and in vitro expression of
integrins and components of extracellular matrix in bovine
placental cells.},
Journal = {Cells, tissues, organs},
Volume = {186},
Number = {4},
Pages = {229-42},
Year = {2007},
ISSN = {1422-6421},
url = {http://dx.doi.org/10.1159/000107947},
Keywords = {Animals • Cattle • Cells, Cultured •
Cytoskeleton • Embryo, Mammalian • Extracellular
Matrix* • Female • Humans • Integrins •
Placenta* • Pregnancy • Protein Subunits •
Trophoblasts • Uterus • chemistry • cytology
• genetics • metabolism • metabolism* •
physiology},
Abstract = {BACKGROUND/AIMS: Interaction of trophoblastic integrins with
the extracellular matrix plays a role in embryo implantation
and trophoblast invasion. The phenomenon of restricted
trophoblast invasion, observed in the bovine
epitheliochorial placenta offers intriguing conditions to
study invasive processes. The migration of bovine
trophoblast giant cells is accompanied by the expression of
specific integrins and corresponding extracellular matrix
ligands. METHODS: Primary cultures of different cell
populations from cow placentomes were established and
characterized, and in vitro phenotypes were compared with in
vivo conditions by immunofluorescence. RESULTS: Propagated
epithelial cells were positive for cytokeratin and vimentin,
while fibroblasts contained alpha-smooth muscle actin,
desmin and vimentin. Epithelial cells coexpressed integrin
subunits alpha(6) and beta(1) with laminin, and fibroblast
cells were positive for alpha(v), beta(3), fibronectin and
laminin. In contrast to cells in vivo, cultured epithelial
cells secreted fibronectin, while collagen IV was not
detected. The occurrence of integrin subunits was confirmed
at mRNA level by RT-PCR. CONCLUSION: We have established
cell cultures isolated from maternal and fetal components of
bovine placentomes expressing typical cytoskeletal filaments
and integrin receptors also present in their in vivo
counterparts. These bovine placentomal cells provide a
suitable in vitro model for the study of cell-cell
interactions.},
Language = {eng},
Doi = {10.1159/000107947},
Key = {fds174226}
}
@booklet{Gray01,
Author = {C. A. Gray and F. F. Bartol and B. J. Tarleton and A. A.
Wiley and G. A. Johnson and F. W. Bazer and T. E.
Spencer},
Title = {Developmental biology of uterine glands},
Journal = {Biology Of Reproduction},
Volume = {65},
Number = {5},
Pages = {1311 -- 1323},
Year = {2001},
Month = {November},
Key = {Gray01}
}
@article{fds174253,
Author = {CA Gray and FF Bartol and BJ Tarleton and AA Wiley and GA Johnson and FW
Bazer, TE Spencer},
Title = {Developmental biology of uterine glands.},
Journal = {Biology of reproduction},
Volume = {65},
Number = {5},
Pages = {1311-23},
Year = {2001},
Month = {November},
ISSN = {0006-3363},
Keywords = {Animals • Endometrium • Estradiol • Female
• Humans • Morphogenesis • Prolactin •
Receptors, Estradiol • Receptors, Prolactin •
Uterus • embryology • embryology* •
physiology},
Abstract = {All mammalian uteri contain endometrial glands that
synthesize or transport and secrete substances essential for
survival and development of the conceptus (embryo/fetus and
associated extraembryonic membranes). In rodents, uterine
secretory products of the endometrial glands are
unequivocally required for establishment of uterine
receptivity and conceptus implantation. Analyses of the
ovine uterine gland knockout model support a primary role
for endometrial glands and, by default, their secretions in
peri-implantation conceptus survival and development.
Uterine adenogenesis is the process whereby endometrial
glands develop. In humans, this process begins in the fetus,
continues postnatally, and is completed during puberty. In
contrast, endometrial adenogenesis is primarily a postnatal
event in sheep, pigs, and rodents. Typically, endometrial
adenogenesis involves differentiation and budding of
glandular epithelium from luminal epithelium, followed by
invagination and extensive tubular coiling and branching
morphogenesis throughout the uterine stroma to the
myometrium. This process requires site-specific alterations
in cell proliferation and extracellular matrix (ECM)
remodeling as well as paracrine cell-cell and cell-ECM
interactions that support the actions of specific hormones
and growth factors. Studies of uterine development in
neonatal ungulates implicate prolactin, estradiol-17 beta,
and their receptors in mechanisms regulating endometrial
adenogenesis. These same hormones appear to regulate
endometrial gland morphogenesis in menstruating primates and
humans during reconstruction of the functionalis from the
basalis endometrium after menses. In sheep and pigs,
extensive endometrial gland hyperplasia and hypertrophy
occur during gestation, presumably to provide increasing
histotrophic support for conceptus growth and development.
In the rabbit, sheep, and pig, a servomechanism is proposed
to regulate endometrial gland development and differentiated
function during pregnancy that involves sequential actions
of ovarian steroid hormones, pregnancy recognition signals,
and lactogenic hormones from the pituitary or placenta. That
disruption of uterine development during critical
organizational periods can alter the functional capacity and
embryotrophic potential of the adult uterus reinforces the
importance of understanding the developmental biology of
uterine glands. Unexplained high rates of peri-implantation
embryonic loss in humans and livestock may reflect defects
in endometrial gland morphogenesis due to genetic errors,
epigenetic influences of endocrine disruptors, and
pathological lesions.},
Language = {eng},
Key = {fds174253}
}
@booklet{Wu03,
Author = {G. Y. Wu and J. T. Self and G. A. Johnson and F. W. Bazer and T. E. Spencer},
Title = {Developmental changes in placental nitric oxide synthesis in
pigs},
Journal = {Biology Of Reproduction},
Volume = {68},
Pages = {153 -- 153},
Year = {2003},
Key = {Wu03}
}
@booklet{Kelly01,
Author = {S. J. Kelly and M. Delnomdedieu and M. I. Oliverio and L. D.
Williams and M. G. P. Saifer and M. R. Sherman and T. M.
Coffman and G. A. Johnson and M. S. Hershfield},
Title = {Diabetes insipidus in uricase-deficient mice: A model for
evaluating therapy with poly(ethylene glycol)-modified
uricase},
Journal = {Journal Of The American Society Of Nephrology},
Volume = {12},
Number = {5},
Pages = {1001 -- 1009},
Year = {2001},
Month = {May},
Key = {Kelly01}
}
@article{fds269101,
Author = {Kelly, SJ and Delnomdedieu, M and Oliverio, MI and Williams, LD and Saifer, MGP and Sherman, MR and Coffman, TM and Johnson, GA and Hershfield, MS},
Title = {Diabetes insipidus in uricase-deficient mice: a model for
evaluating therapy with poly(ethylene glycol)-modified
uricase.},
Journal = {J Am Soc Nephrol},
Volume = {12},
Number = {5},
Pages = {1001-1009},
Year = {2001},
Month = {May},
ISSN = {1046-6673},
url = {http://www.ncbi.nlm.nih.gov/pubmed/11316859},
Keywords = {Animals • Body Water • Diabetes Insipidus •
Disease Models, Animal • Gout • Humans •
Kidney Concentrating Ability • Mice • Mice, Inbred
C57BL • Mice, Knockout • Polyethylene Glycols
• Recombinant Proteins • Urate Oxidase • Uric
Acid • deficiency* • drug therapy • drug
therapy* • enzymology* • genetics •
metabolism • pathology • physiopathology •
therapeutic use • therapeutic use* •
urine},
Abstract = {Uricase-deficient mice develop uric acid nephropathy, with
high mortality rates before weaning. Urate excretion was
quantitated and renal function was better defined in this
study, to facilitate the use of these mice as a model for
evaluating poly(ethylene glycol)-modified recombinant
mammalian uricases (PEG-uricase) as a potential therapy for
gout and uric acid nephropathy. The uric acid/creatinine
ratio in the urine of uricase-deficient mice ranges from 10
to >30; on a weight basis, these mice excrete 20- to 40-fold
more urate than do human subjects. These mice consistently
develop a severe defect in renal concentrating ability,
resulting in an approximately sixfold greater urine volume
and a fivefold greater fluid requirement, compared with
normal mice. This nephrogenic diabetes insipidus leads to
dehydration and death of nursing mice but, with adequate
water replacement, high urine flow protects adults from
progressive renal damage. Treatment of uricase-deficient
mice with PEG-uricase markedly reduced urate levels and,
when initiated before weaning, preserved the renal
architecture (as evaluated by magnetic resonance
micros-copy) and prevented the loss of renal concentrating
function. PEG-uricase was far more effective and less
immunogenic than unmodified uricase. Retention of uricase in
most mammals and its loss in humans and some other primates
may reflect the evolution of renal function under different
environmental conditions. PEG-uricase could provide an
effective therapy for uric acid nephropathy and refractory
gout in human patients.},
Doi = {10.1681/ASN.V1251001},
Key = {fds269101}
}
@article{fds174151,
Author = {X Li and FW Bazer and GA Johnson and RC Burghardt and DW Erikson and JW
Frank, TE Spencer and I Shinzato and G Wu},
Title = {Dietary Supplementation with 0.8% L-Arginine between Days 0
and 25 of Gestation Reduces Litter Size in
Gilts.},
Journal = {The Journal of nutrition},
Year = {2010},
Month = {April},
ISSN = {1541-6100},
url = {http://dx.doi.org/10.3945/jn.110.121350},
Abstract = {In this study, we determined the effects of l-arginine
supplementation during early pregnancy on embryonic/fetal
survival and growth in gilts. Gilts were housed individually
in pens and fed twice daily 1 kg of a corn- and soybean
meal-based diet supplemented with 0.0, 0.4, or 0.8%
l-arginine (wt:wt) between d 0 and 25 of gestation (10
gilts/treatment). The diets were made isonitrogenous by
addition of appropriate amounts of l-alanine. At d 25 of
gestation, gilts were fed l-alanine or l-arginine and
hysterectomized 30 min later to obtain uteri and conceptuses
(embryos and associated fetal membranes and fluids). Dietary
supplementation with 0.4 or 0.8% l-arginine enhanced (P <
0.05) its concentrations in maternal plasma (64 and 98%,
respectively) as well as the vascularity of chorionic and
allantoic membranes, compared with the control group.
Reproductive performance [numbers of corpora lutea (CL) and
fetuses, placental and fetal weights, and embryonic
mortality] did not differ between the 0.4% Arg and control
groups. However, supplementation with 0.8% l-arginine
decreased (P < 0.05) uterine weight (-20%), total number of
fetuses (-24%), CL number (-17%), total fetal weight (-34%),
total volume of allantoic and amniotic fluids (-34 to 42%),
concentrations of progesterone in maternal plasma (-33%), as
well as total amounts of progesterone (-35%), estrone
(-40%), and estrone sulfate (-37%) in allantoic fluid,
compared with the control group. These results indicate that
dietary supplementation with 0.8% l-arginine between d 0 and
25 of gestation, while increasing placental vascularity,
adversely affects the reproductive performance of
gilts.},
Language = {ENG},
Doi = {10.3945/jn.110.121350},
Key = {fds174151}
}
@article{fds174309,
Author = {GA Johnson and EG Kim and W Veldkamp and R Russell},
Title = {Difference in oral effectiveness of two tyrosine hydroxylase
inhibitors.},
Journal = {Biochemical pharmacology},
Volume = {16},
Number = {2},
Pages = {401-3},
Year = {1967},
Month = {February},
ISSN = {0006-2952},
Keywords = {Animals • Body Weight • Brain • Brain
Chemistry* • Dextroamphetamine • Diet •
Dopamine • Eating • Enzymes • Male •
Methyltyrosines • Mice • Movement •
Norepinephrine • Oxidoreductases • Reserpine
• Serotonin • Tyrosine • analysis • drug
effects • pharmacology • pharmacology*},
Language = {eng},
Key = {fds174309}
}
@booklet{Spencer99a,
Author = {T. E. Spencer and A. G. Stagg and T. L. Ott and G. A.
Johnson and W. S. Ramsey and F. W. Bazer},
Title = {Differential effects of intrauterine and subcutaneous
administration of recombinant ovine interferon tau on the
endometrium of cyclic ewes},
Journal = {Biology Of Reproduction},
Volume = {61},
Number = {2},
Pages = {464 -- 470},
Year = {1999},
Month = {August},
Key = {Spencer99a}
}
@article{fds174157,
Author = {TE Spencer and AG Stagg and TL Ott and GA Johnson and WS Ramsey and FW
Bazer},
Title = {Differential effects of intrauterine and subcutaneous
administration of recombinant ovine interferon tau on the
endometrium of cyclic ewes.},
Journal = {Biology of reproduction},
Volume = {61},
Number = {2},
Pages = {464-70},
Year = {1999},
Month = {August},
ISSN = {0006-3363},
Keywords = {Animals • Antiviral Agents • Carrier Proteins
• Endometrium • Female • GTP
Phosphohydrolases • GTP-Binding Proteins • Gene
Expression Regulation, Developmental • In Situ
Hybridization • Injections, Subcutaneous •
Interferon Type I • Leucine Zippers • Pregnancy
• Pregnancy Proteins • Protein Biosynthesis •
Proteins • Receptors, Estrogen • Receptors,
Oxytocin • Recombinant Proteins • Sheep •
Ubiquitins • Uterus • administration & dosage
• administration & dosage* • analogs & derivatives
• biosynthesis • drug effects* • genetics
• pharmacology • pharmacology*},
Abstract = {Interferon tau (IFNtau) is the antiluteolytic signal
produced by the conceptus of ruminants. Intrauterine
administration of recombinant ovine IFNtau suppresses
expression of endometrial estrogen receptor (ER) and
oxytocin receptor (OTR) in the luminal and superficial
glandular epithelia to abrogate the production of luteolytic
prostaglandin F(2alpha) (PGF(2alpha)) pulses. Subcutaneous
(s.c.) injections of recombinant ovine (o) IFNtau appear to
extend the interestrous interval by altering uterine
PGF(2alpha) response to oxytocin. The present study tested
the hypothesis that antiluteolytic effects of roIFNtau
injected into the uterine lumen (paracrine) or s.c.
(endocrine) are equivalent in suppressing expression of
endometrial ER and OTR and inducing uterine expression of
type I IFN-regulated Mx and ubiquitin cross-reactive
proteins (UCRP). Sixteen cyclic ewes were fitted with
uterine catheters on Day 5 (Day 0 = estrus), were assigned
randomly to receive treatment with control proteins or
roIFNtau (2 x 10(7) antiviral units/day) by either
intrauterine or s.c. injections from Days 11 to 15, and were
ovariohysterectomized on Day 16. Results indicated that
expression of ER and OTR mRNAs in endometrial epithelium was
suppressed by intrauterine but not by s.c. injections of
roIFNtau. Intrauterine injections of roIFNtau increased
expression of Mx and UCRP mRNA in the endometrium.
Subcutaneous injections of roIFNtau increased endometrial Mx
mRNA levels but not UCRP mRNA. Unexpectedly, intrauterine
and s.c. injections of roIFNtau were equally effective in
inducing expression of Mx and UCRP mRNA in the corpus
luteum. Although s.c. injections of roIFNtau induced Mx mRNA
in the endometrial epithelium, s.c. injections of roIFNtau
did not abrogate activation of the uterine luteolytic
mechanism by suppressing epithelial ER and OTR expression.
Therefore, results of this study failed to support the
assumption that endocrine roIFNtau mimics antiluteolytic
effects of paracrine IFNtau to improve pregnancy rates in
sheep.},
Language = {eng},
Key = {fds174157}
}
@article{fds132763,
Author = {RH Sherrier and C Chiles and GA Johnson and CE Ravin},
Title = {Differentiation of benign from malignant pulmonary nodules
with digitized chest radiographs.},
Journal = {Radiology, UNITED STATES},
Volume = {162},
Number = {3},
Pages = {645-9},
Year = {1987},
Month = {March},
ISSN = {0033-8419},
Keywords = {Coin Lesion, Pulmonary • Diagnosis, Differential •
Humans • Lung Neoplasms • Radiographic Image
Enhancement • methods* • radiography •
radiography*},
Abstract = {To assess whether it is possible to distinguish benign from
malignant solitary pulmonary nodules with digital
techniques, a retrospective study of 68 patients with proved
solitary nodules was performed. The conventional chest
radiograph for each patient was digitized to 2,048 X 2,048 X
12 bits, and changes in the optical density within the
nodule were analyzed. A number (the corrected gradient
number) was then generated that reflected this variation.
Striking differences were noted between 26 malignant nodules
and 21 calcified granulomas. The technique was then applied
to 21 benign nodules that had initially required thoracotomy
or further study for diagnosis. In nine of these 21 patients
(43%), the corrected gradient number allowed correct
classification as a benign lesion.},
Key = {fds132763}
}
@booklet{Sherrier87a,
Author = {Sherrier, RH and Chiles, C and Johnson, GA and Ravin,
CE},
Title = {Differentiation of benign from malignant pulmonary nodules
with digitized chest radiographs.},
Journal = {Radiology},
Volume = {162},
Number = {3},
Pages = {645-649},
Year = {1987},
Month = {March},
ISSN = {0033-8419},
url = {http://www.ncbi.nlm.nih.gov/pubmed/3809476},
Abstract = {To assess whether it is possible to distinguish benign from
malignant solitary pulmonary nodules with digital
techniques, a retrospective study of 68 patients with proved
solitary nodules was performed. The conventional chest
radiograph for each patient was digitized to 2,048 X 2,048 X
12 bits, and changes in the optical density within the
nodule were analyzed. A number (the corrected gradient
number) was then generated that reflected this variation.
Striking differences were noted between 26 malignant nodules
and 21 calcified granulomas. The technique was then applied
to 21 benign nodules that had initially required thoracotomy
or further study for diagnosis. In nine of these 21 patients
(43%), the corrected gradient number allowed correct
classification as a benign lesion.},
Doi = {10.1148/radiology.162.3.3809476},
Key = {Sherrier87a}
}
@booklet{Macfall91b,
Author = {J. R. Macfall and J. H. Maki and G. A. Johnson and L.
Hedlund and H. Benveniste and G. Copher},
Title = {Diffusion microcirculation mri in the rat-brain},
Journal = {Magnetic Resonance In Medicine},
Volume = {19},
Number = {2},
Pages = {305 -- 310},
Year = {1991},
Month = {June},
Key = {Macfall91b}
}
@article{fds268743,
Author = {O'Leary-Moore, SK and Johnson, GA and Calabrese, E and Budin, F and Oguz, I and Styner, MA and Parnell, SE and Sulik,
KK},
Title = {DIFFUSION TENSOR IMAGING (DTI)-BASED ANALYSIS OF FIBER TRACT
ABNORMALITIES IN A MOUSE MODEL OF PRENATAL ALCOHOL
EXPOSURE},
Journal = {ALCOHOLISM-CLINICAL AND EXPERIMENTAL RESEARCH},
Volume = {36},
Pages = {312A-312A},
Year = {2012},
ISSN = {0145-6008},
url = {http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000304806002399&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=47d3190e77e5a3a53558812f597b0b92},
Key = {fds268743}
}
@article{fds268723,
Author = {Calabrese, E and Du, F and Garman, RH and Johnson, GA and Riccio, C and Tong, LC and Long, JB},
Title = {Diffusion tensor imaging reveals white matter injury in a
rat model of repetitive blast-induced traumatic brain
injury.},
Journal = {J Neurotrauma},
Volume = {31},
Number = {10},
Pages = {938-950},
Year = {2014},
Month = {May},
url = {http://www.ncbi.nlm.nih.gov/pubmed/24392843},
Abstract = {Blast-induced traumatic brain injury (bTBI) is one of the
most common combat-related injuries seen in U.S. military
personnel, yet relatively little is known about the
underlying mechanisms of injury. In particular, the effects
of the primary blast pressure wave are poorly understood.
Animal models have proven invaluable for the study of
primary bTBI, because it rarely occurs in isolation in human
subjects. Even less is known about the effects of repeated
primary blast wave exposure, but existing data suggest
cumulative increases in brain damage with a second blast.
MRI and, in particular, diffusion tensor imaging (DTI), have
become important tools for assessing bTBI in both clinical
and preclinical settings. Computational statistical methods
such as voxelwise analysis have shown promise in localizing
and quantifying bTBI throughout the brain. In this study, we
use voxelwise analysis of DTI to quantify white matter
injury in a rat model of repetitive primary blast exposure.
Our results show a significant increase in microstructural
damage with a second blast exposure, suggesting that primary
bTBI may sensitize the brain to subsequent
injury.},
Doi = {10.1089/neu.2013.3144},
Key = {fds268723}
}
@article{fds336018,
Author = {Nouls, JC and Badea, A and Anderson, RBJ and Cofer, GP and Allan
Johnson, G},
Title = {Diffusion tensor imaging using multiple coils for mouse
brain connectomics.},
Journal = {NMR Biomed},
Volume = {31},
Number = {6},
Pages = {e3921},
Year = {2018},
Month = {June},
url = {http://dx.doi.org/10.1002/nbm.3921},
Abstract = {The correlation between brain connectivity and psychiatric
or neurological diseases has intensified efforts to develop
brain connectivity mapping techniques on mouse models of
human disease. The neural architecture of mouse brain
specimens can be shown non-destructively and
three-dimensionally by diffusion tensor imaging, which
enables tractography, the establishment of a connectivity
matrix and connectomics. However, experiments on cohorts of
animals can be prohibitively long. To improve throughput in
a 7-T preclinical scanner, we present a novel two-coil
system in which each coil is shielded, placed off-isocenter
along the axis of the magnet and connected to a receiver
circuit of the scanner. Preservation of the quality factor
of each coil is essential to signal-to-noise ratio (SNR)
performance and throughput, because mouse brain specimen
imaging at 7 T takes place in the coil-dominated noise
regime. In that regime, we show a shielding configuration
causing no SNR degradation in the two-coil system. To
acquire data from several coils simultaneously, the coils
are placed in the magnet bore, around the isocenter, in
which gradient field distortions can bias diffusion tensor
imaging metrics, affect tractography and contaminate
measurements of the connectivity matrix. We quantified the
experimental alterations in fractional anisotropy and
eigenvector direction occurring in each coil. We showed
that, when the coils were placed 12 mm away from the
isocenter, measurements of the brain connectivity matrix
appeared to be minimally altered by gradient field
distortions. Simultaneous measurements on two mouse brain
specimens demonstrated a full doubling of the diffusion
tensor imaging throughput in practice. Each coil produced
images devoid of shading or artifact. To further improve the
throughput of mouse brain connectomics, we suggested a
future expansion of the system to four coils. To better
understand acceptable trade-offs between imaging throughput
and connectivity matrix integrity, studies may seek to
clarify how measurement variability, post-processing
techniques and biological variability impact mouse brain
connectomics.},
Doi = {10.1002/nbm.3921},
Key = {fds336018}
}
@article{fds268736,
Author = {Calabrese, E and Johnson, GA},
Title = {Diffusion tensor magnetic resonance histology reveals
microstructural changes in the developing rat
brain.},
Journal = {Neuroimage},
Volume = {79},
Pages = {329-339},
Year = {2013},
Month = {October},
url = {http://www.ncbi.nlm.nih.gov/pubmed/23648962},
Abstract = {The postnatal period is a remarkably dynamic phase of brain
growth and development characterized by large-scale
macrostructural changes, as well as dramatic microstructural
changes, including myelination and cortical layering. This
crucial period of neurodevelopment is uniquely susceptible
to a wide variety of insults that may lead to neurologic
disease. MRI is an important tool for studying both normal
and abnormal neurodevelopmental changes, and quantitative
imaging strategies like diffusion tensor imaging (DTI) allow
visualization of many of the complex microstructural changes
that occur during postnatal life. Diffusion tensor magnetic
resonance histology (DT-MRH) provides particularly unique
insight into cytoarchitectural changes in the developing
brain. In this study, we used DT-MRH to track
microstructural changes in the rat brain throughout normal
postnatal neurodevelopment. We provide examples of diffusion
tensor parameter changes in both white matter and gray
matter structures, and correlate these changes with changes
in cytoarchitecture. Finally, we provide a comprehensive
database of image sets as a foundation for future studies
using DT-MRH to characterize abnormal neurodevelopment in
rodent models of neurodevelopmental disease.},
Doi = {10.1016/j.neuroimage.2013.04.101},
Key = {fds268736}
}
@article{fds341985,
Author = {Wang, N and Mirando, AJ and Cofer, G and Qi, Y and Hilton, MJ and Johnson,
GA},
Title = {Diffusion tractography of the rat knee at microscopic
resolution.},
Journal = {Magn Reson Med},
Volume = {81},
Number = {6},
Pages = {3775-3786},
Year = {2019},
Month = {June},
url = {http://dx.doi.org/10.1002/mrm.27652},
Abstract = {PURPOSE: To evaluate whole knee joint tractography,
including articular cartilage, ligaments, meniscus, and
growth plate using diffusion tensor imaging (DTI) at
microscopic resolution. METHODS: Three rat knee joints were
scanned using a modified 3D diffusion-weighted spin echo
pulse sequence with 90- and 45-μm isotropic spatial
resolution at 9.4T. The b values varied from 250 to 1250
s/mm2 with 4 times undersampling in phase directions.
Fractional anisotropy (FA) and mean diffusivity (MD) were
compared at different spatial resolution and b values.
Tractography was evaluated at multiple b values and angular
resolutions in different connective tissues, and compared
with conventional histology. The mean tract length and tract
volume in various types of tissues were also quantified.
RESULTS: DTI metrics (FA and MD) showed consistent
quantitative results at 90- and 45-μm isotropic spatial
resolutions. Tractography of various connective tissues was
found to be sensitive to the spatial resolution, angular
resolution, and diffusion weightings. Higher spatial
resolution (45 μm) supported tracking the cartilage
collagen fiber tracts from the superficial zone to the deep
zone, in a continuous and smooth progression in the
transitional zone. Fiber length and fiber volume in the
growth plate were strongly dependent on angular resolution
and b values, whereas tractography in ligaments was found to
be less dependent on spatial resolution. CONCLUSION: High
spatial and angular resolution DTI and diffusion
tractography can be valuable for knee joint research because
of its visualization capacity for collagen fiber
orientations and quantitative evaluation of tissue's
microscopic properties.},
Doi = {10.1002/mrm.27652},
Key = {fds341985}
}
@article{fds132826,
Author = {CF Beaulieu and X Zhou and GP Cofer and GA Johnson},
Title = {Diffusion-weighted MR microscopy with fast
spin-echo.},
Journal = {Magnetic resonance in medicine : official journal of the
Society of Magnetic Resonance in Medicine / Society of
Magnetic Resonance in Medicine, UNITED STATES},
Volume = {30},
Number = {2},
Pages = {201-6},
Year = {1993},
Month = {August},
ISSN = {0740-3194},
Keywords = {Animals • Diffusion • Image Processing,
Computer-Assisted • Magnetic Resonance Imaging •
Microscopy • Models, Structural • Rats •
methods*},
Abstract = {A diffusion-weighted fast spin-echo (FSE) imaging sequence
for high-field MR microscopy was developed and
experimentally validated in a phantom and in a live rat.
Pulsed diffusion gradients were executed before and after
the initial 180 degrees pulse in the FSE pulse train. This
produced diffusion-related reductions in image signal
intensity corresponding to gradient ("b") factors between
1.80 and 1352 s/mm2. The degree of diffusion weighting was
demonstrated to be independent of echo train length for
experiments using trains up to 16 echoes long. Quantitative
measurements on a phantom and on a live rat produced
diffusion coefficients consistent with literature values.
Importantly, the eight- to 16-fold increase in imaging
efficiency with FSE was not accompanied by a significant
loss of spatial resolution or contrast. This permits
acquisition of in vivo three-dimensional data in time
periods that are appropriate for evolving biological
processes. The combination of accurate diffusion weighting
and high spatial resolution provided by FSE makes the
technique particularly useful for MR microscopy.},
Key = {fds132826}
}
@booklet{Beaulieu93,
Author = {Beaulieu, CF and Zhou, X and Cofer, GP and Johnson,
GA},
Title = {Diffusion-weighted MR microscopy with fast
spin-echo.},
Journal = {Magn Reson Med},
Volume = {30},
Number = {2},
Pages = {201-206},
Year = {1993},
Month = {August},
ISSN = {0740-3194},
url = {http://www.ncbi.nlm.nih.gov/pubmed/8366801},
Abstract = {A diffusion-weighted fast spin-echo (FSE) imaging sequence
for high-field MR microscopy was developed and
experimentally validated in a phantom and in a live rat.
Pulsed diffusion gradients were executed before and after
the initial 180 degrees pulse in the FSE pulse train. This
produced diffusion-related reductions in image signal
intensity corresponding to gradient ("b") factors between
1.80 and 1352 s/mm2. The degree of diffusion weighting was
demonstrated to be independent of echo train length for
experiments using trains up to 16 echoes long. Quantitative
measurements on a phantom and on a live rat produced
diffusion coefficients consistent with literature values.
Importantly, the eight- to 16-fold increase in imaging
efficiency with FSE was not accompanied by a significant
loss of spatial resolution or contrast. This permits
acquisition of in vivo three-dimensional data in time
periods that are appropriate for evolving biological
processes. The combination of accurate diffusion weighting
and high spatial resolution provided by FSE makes the
technique particularly useful for MR microscopy.},
Doi = {10.1002/mrm.1910300208},
Key = {Beaulieu93}
}
@article{fds269122,
Author = {MacFall, JR and Maki, JH and Johnson, GA and Hedlund, L and Benveniste,
H and Copher, G},
Title = {Diffusion/microcirculation MRI in the rat
brain.},
Journal = {Magn Reson Med},
Volume = {19},
Number = {2},
Pages = {305-310},
Year = {1991},
Month = {June},
ISSN = {0740-3194},
url = {http://www.ncbi.nlm.nih.gov/pubmed/1908936},
Keywords = {Animals • Brain • Carbon Dioxide •
Cerebrovascular Circulation • Diffusion • Magnetic
Resonance Imaging* • Microcirculation • Rats
• Rats, Inbred Strains • administration & dosage
• anatomy & histology • blood supply •
metabolism • pharmacokinetics •
physiology*},
Abstract = {The CO2 fraction of an anesthetized rat's breathing mixture
was changed (from 0 to 10%) to attempt to change the brain
microcirculation and observe these changes in diffusion
measurements of the neural tissue. Brain apparent diffusion
coefficients were measured to be (0.71 +/- 0.01) X 10(-3)
mm2/s before sacrifice and (0.39 +/- 0.01) X 10(-3) mm2/s
after sacrifice. Multiple diffusion components were
observed, consistent with flowing material, but the extra
components did not increase with increased CO2. It is
proposed that the additional components may be due to
extracellular, extravascular water such as
CSF.},
Doi = {10.1002/mrm.1910190219},
Key = {fds269122}
}
@article{fds268821,
Author = {Hawrylycz, M and Baldock, RA and Burger, A and Hashikawa, T and Johnson,
GA and Martone, M and Ng, L and Lau, C and Larson, SD and Nissanov, J and Puelles, L and Ruffins, S and Verbeek, F and Zaslavsky, I and Boline,
J},
Title = {Digital atlasing and standardization in the mouse
brain.},
Journal = {PLoS Comput Biol},
Volume = {7},
Number = {2},
Pages = {e1001065},
Year = {2011},
Month = {February},
ISSN = {1553-7358},
url = {http://www.ncbi.nlm.nih.gov/pubmed/21304938},
Keywords = {Anatomy, Artistic • Animals • Atlases as Topic
• Brain • Computational Biology • Male •
Mice • Mice, Inbred C57BL • Models, Anatomic*
• Models, Neurological • anatomy & histology*
• standards • statistics & numerical
data},
Language = {eng},
Doi = {10.1371/journal.pcbi.1001065},
Key = {fds268821}
}
@article{fds174203,
Author = {RM Vandemark and ME Fay and FR Porter and GA Johnson},
Title = {Digital image-intensifier radiography at a level I trauma
center.},
Journal = {AJR. American journal of roentgenology},
Volume = {168},
Number = {4},
Pages = {944-6},
Year = {1997},
Month = {April},
ISSN = {0361-803X},
Keywords = {Humans • Pelvis • Radiographic Image Enhancement
• Radiography, Thoracic • Spinal Injuries •
Spine • Trauma Centers • Wounds and Injuries
• injuries • instrumentation* •
radiography},
Language = {eng},
Key = {fds174203}
}
@booklet{Vandemark97,
Author = {Vandemark, RM and Fay, ME and Porter, FR and Johnson,
GA},
Title = {Digital image-intensifier radiography at a level I trauma
center.},
Journal = {AJR Am J Roentgenol},
Volume = {168},
Number = {4},
Pages = {944-946},
Year = {1997},
Month = {April},
ISSN = {0361-803X},
url = {http://www.ncbi.nlm.nih.gov/pubmed/9124145},
Doi = {10.2214/ajr.168.4.9124145},
Key = {Vandemark97}
}
@booklet{Dunnick85,
Author = {N. R. Dunnick and K. K. Ford and G. A. Johnson and J. C.
Gunnells},
Title = {Digital intravenous subtraction angiography for
investigating renovascular hypertension - comparison with
hypertensive urography},
Journal = {Southern Medical Journal},
Volume = {78},
Number = {6},
Pages = {690 -- 693},
Year = {1985},
Key = {Dunnick85}
}
@article{fds132748,
Author = {NR Dunnick and KK Ford and GA Johnson and JC Gunnells},
Title = {DIgital intravenous subtraction angiography for
investigating renovascular hypertension: comparison with
hypertensive urography.},
Journal = {Southern medical journal, UNITED STATES},
Volume = {78},
Number = {6},
Pages = {690-3},
Year = {1985},
Month = {June},
ISSN = {0038-4348},
Keywords = {Adolescent • Adult • Aged • Female •
Humans • Hypertension • Hypertension, Renovascular
• Male • Middle Aged • Renal Artery •
Renin • Subtraction Technique* • Urography* •
blood • diagnosis • radiography •
radiography*},
Abstract = {We used digital intravenous subtraction angiography (DSA) to
evaluate 105 patients with suspected renovascular
hypertension. Unilateral renal artery stenoses were
identified in 14 patients, two of whom had previously had
contralateral nephrectomy. In addition, one of three renal
transplant recipients was found to have stenosis of the
nutrient artery. Bilateral renal artery stenosis was
demonstrated by DSA in three patients. Of the 88 patients
who had concomitant minute sequence (hypertensive) urography
(HIVP) delayed excretion suggested a renal artery lesion in
only 8 patients. In the group of 88 patients, HIVP was able
to detect renal artery stenosis in only 50% (eight of the
16) of patients whose stenosis was detected by DSA. When the
patients with a single kidney are excluded, HIVP showed 62%
(eight of 13) of the lesions detected by DSA. There were no
significant complications in the patients examined by either
modality. DSA has replaced HIVP as the screening examination
for renovascular causes of hypertension in our
institution.},
Key = {fds132748}
}
@booklet{Johnson81,
Author = {Johnson, GA and Ford, K and Heinz, R},
Title = {Digital radiography using the quantex ds-20},
Journal = {Proceedings of SPIE - The International Society for Optical
Engineering},
Volume = {273},
Pages = {114-119},
Year = {1981},
Month = {July},
url = {http://dx.doi.org/10.1117/12.931792},
Abstract = {The Quantex DS-20 digital image processor has been evaluated
as a low cost alternative for digital vascular radiography.
The processor is used in conjunction with a conventional
remote radiographic/fluoroscopic system with no
generator/processor interface required. (Use of a video-tape
system reduces timing problems with minimal image
degradation). Phantom studies indicate vessels as small as
1.5 mm can be seen with concentrations of contrast as low as
10 mgγ/ml. Animal studies show that subtraction images of
diagnostic quality can be generated. © 1981
SPIE.},
Doi = {10.1117/12.931792},
Key = {Johnson81}
}
@booklet{Johnson81e,
Author = {JOHNSON, GA and FORD, KK and HEINZ, R},
Title = {DIGITAL RADIOGRAPHY USING THE QUANTEX DS-20},
Journal = {AMERICAN JOURNAL OF ROENTGENOLOGY},
Volume = {136},
Number = {6},
Pages = {1274-1274},
Year = {1981},
ISSN = {0361-803X},
url = {http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:A1981LU08900069&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=47d3190e77e5a3a53558812f597b0b92},
Key = {Johnson81e}
}
@article{fds292751,
Author = {Spiliopoulos, D and Kagadis, GC and Karnabatidis, D and Johnson, GA and Badea, CT},
Title = {Digital Subtracted Angiography of Small Animals},
Pages = {67-75},
Booktitle = {Handbook of Small Animal Imaging: Preclinical Imaging,
Therapy, and Applications},
Publisher = {Taylor & Francis Books, Inc., CRC Press},
Editor = {Kagadis, GC and Ford, NL and Loudos, GK and Karnabatidis,
D},
Year = {2016},
Month = {March},
ISBN = {978-1466555686},
url = {http://dx.doi.org/10.1201/b19052-9},
Doi = {10.1201/b19052-9},
Key = {fds292751}
}
@article{fds132843,
Author = {RH Sherrier and GA Johnson and SA Suddarth and C Chiles and C Hulka and CE
Ravin},
Title = {Digital synthesis of lung nodules.},
Journal = {Investigative radiology, UNITED STATES},
Volume = {20},
Number = {9},
Pages = {933-7},
Year = {1985},
Month = {December},
ISSN = {0020-9996},
Keywords = {Coin Lesion, Pulmonary • Humans • Lung Neoplasms
• Radiography, Thoracic • Software • methods*
• radiography*},
Abstract = {Studies evaluating observer accuracy and visual perception
of pulmonary nodules usually are based upon test films
obtained from clinical practice in patients with proven
pulmonary nodules. Unfortunately, such nodules do not always
occur in the optimal size and location to facilitate
testing. Such studies would be enhanced by the ability to
place nodules of desired size and location on chest
radiographs. This report describes a method of placing a
computer-generated (synthesized) nodule on a digitized chest
radiograph. To demonstrate the similarity of these
synthesized nodules to real nodules, each digitized
radiograph with a computer-generated nodule was paired with
a digitized chest radiograph of a patient with a clinically
proven pulmonary nodule. A total of 22 pairs of chest
radiographs were then shown to 13 radiologists, who were
asked to distinguish the synthesized nodule from the real
nodule. With this two alternative forced-choice test, the
radiologists were only able to distinguish the synthesized
nodule in 51% of the cases, strongly suggesting that
computer generated nodules may be used to simulate real
pulmonary nodules in future tests of nodule
detection.},
Key = {fds132843}
}
@booklet{Sherrier85,
Author = {Sherrier, RH and Johnson, GA and Suddarth, SA and Chiles, C and Hulka,
C and Ravin, CE},
Title = {Digital synthesis of lung nodules.},
Journal = {Invest Radiol},
Volume = {20},
Number = {9},
Pages = {933-937},
Year = {1985},
Month = {December},
ISSN = {0020-9996},
url = {http://www.ncbi.nlm.nih.gov/pubmed/3841098},
Abstract = {Studies evaluating observer accuracy and visual perception
of pulmonary nodules usually are based upon test films
obtained from clinical practice in patients with proven
pulmonary nodules. Unfortunately, such nodules do not always
occur in the optimal size and location to facilitate
testing. Such studies would be enhanced by the ability to
place nodules of desired size and location on chest
radiographs. This report describes a method of placing a
computer-generated (synthesized) nodule on a digitized chest
radiograph. To demonstrate the similarity of these
synthesized nodules to real nodules, each digitized
radiograph with a computer-generated nodule was paired with
a digitized chest radiograph of a patient with a clinically
proven pulmonary nodule. A total of 22 pairs of chest
radiographs were then shown to 13 radiologists, who were
asked to distinguish the synthesized nodule from the real
nodule. With this two alternative forced-choice test, the
radiologists were only able to distinguish the synthesized
nodule in 51% of the cases, strongly suggesting that
computer generated nodules may be used to simulate real
pulmonary nodules in future tests of nodule
detection.},
Doi = {10.1097/00004424-198512000-00008},
Key = {Sherrier85}
}
@article{fds268753,
Author = {Perez, BA and Ghafoori, AP and Johnston, SM and Jeffords, LB and Kim, Y and Badea, CT and Johnson, GA and Kirsch, DG},
Title = {Dissecting the Mechanism of Tumor Response to Radiation
Therapy with Primary Lung Cancers in Mice},
Journal = {International Journal of Radiation Oncology*Biology*Physics},
Volume = {75},
Number = {3},
Pages = {S537-S537},
Publisher = {Elsevier BV},
Year = {2009},
Month = {November},
ISSN = {0360-3016},
url = {http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000270573602102&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=47d3190e77e5a3a53558812f597b0b92},
Doi = {10.1016/j.ijrobp.2009.07.1227},
Key = {fds268753}
}
@article{fds132824,
Author = {JD Godwin and JM Speckman and EK Fram and GA Johnson and CE Putman and M
Korobkin, RS Breiman},
Title = {Distinguishing benign from malignant pulmonary nodules by
computed tomography.},
Journal = {Radiology, UNITED STATES},
Volume = {144},
Number = {2},
Pages = {349-51},
Year = {1982},
Month = {July},
ISSN = {0033-8419},
Keywords = {Calcinosis • Coin Lesion, Pulmonary • Humans
• Lung Neoplasms • Technology, Radiologic •
Tomography, X-Ray Computed* • radiography*},
Abstract = {Investigators have been able to distinguish benign pulmonary
nodules from malignant ones in about two-thirds of the cases
studied by detecting high computed tomography (CT) numbers
(attributed to microscopic calcifications) within many
benign nodules. This paper reports a similar analysis on a
series of 22 benign and 14 malignant pulmonary nodules.
Although about one-third of the benign nodules gave high CT
numbers, all but one of the nodules diagnosed as benign by
CT could also be diagnosed by detection of calcification on
plain radiographs or conventional tomograms.},
Key = {fds132824}
}
@booklet{Godwin82,
Author = {Godwin, JD and Speckman, JM and Fram, EK and Johnson, GA and Putman, CE and Korobkin, M and Breiman, RS},
Title = {Distinguishing benign from malignant pulmonary nodules by
computed tomography.},
Journal = {Radiology},
Volume = {144},
Number = {2},
Pages = {349-351},
Year = {1982},
Month = {July},
ISSN = {0033-8419},
url = {http://www.ncbi.nlm.nih.gov/pubmed/7089288},
Abstract = {Investigators have been able to distinguish benign pulmonary
nodules from malignant ones in about two-thirds of the cases
studied by detecting high computed tomography (CT) numbers
(attributed to microscopic calcifications) within many
benign nodules. This paper reports a similar analysis on a
series of 22 benign and 14 malignant pulmonary nodules.
Although about one-third of the benign nodules gave high CT
numbers, all but one of the nodules diagnosed as benign by
CT could also be diagnosed by detection of calcification on
plain radiographs or conventional tomograms.},
Doi = {10.1148/radiology.144.2.7089288},
Key = {Godwin82}
}
@booklet{Veres91,
Author = {Veres, JS and Cofer, GP and Johnson, GA},
Title = {Distinguishing Plant Tissues with Magnetic Resonance
Microscopy},
Journal = {American Journal of Botany},
Volume = {78},
Number = {12},
Pages = {1704-1704},
Publisher = {Wiley},
Year = {1991},
Month = {December},
ISSN = {0002-9122},
url = {http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:A1991GY25000009&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=47d3190e77e5a3a53558812f597b0b92},
Doi = {10.2307/2444849},
Key = {Veres91}
}
@article{fds325756,
Author = {Wetzel, AW and Pomerantz, S and Nave, D and Meixner, W and Johnson,
GA},
Title = {Distributed multiuser visualization of time varying
anatomical data},
Journal = {Proceedings - Applied Imagery Pattern Recognition
Workshop},
Volume = {2001-January},
Pages = {109-114},
Publisher = {IEEE Comput. Soc},
Year = {2001},
Month = {January},
ISBN = {0769512453},
url = {http://dx.doi.org/10.1109/AIPR.2001.991211},
Abstract = {We describe a networked environment for navigating and
visualizing 3-dimensional anatomical data with extensions
for time varying volumes. The Duke Center for In Vivo
Microscopy (CIVM) has been capturing volumetric data of mice
using magnetic resonance microscopy. Current data sets are
51Z3 with 16 bit precision per voxel at an isotropic
resolution of 50 microns. A new instrument will provide
larger 512 ∗ 512 ∗ 2048 volumes. Because magnetic
resonance imaging is nondestructive, both rapid time series
and longer interval developmental series can be taken from
living specimens. Our work builds on techniques put in place
at the Pittsburgh Supercomputing Center and the University
of Michigan for navigating Visible Human data using a
client-server implementation, but applied to CIVM mouse
data. Extension of the system to 4-dimensional data sets
involves changes to compressed data representations and
client viewing mechanisms. An essential aspect of the mouse
studies is to facilitate comparison between different
specimens, or even the same specimen over time, for studies
of morphologic phenotype expression in gene
knockouts.},
Doi = {10.1109/AIPR.2001.991211},
Key = {fds325756}
}
@booklet{Al-ramadan02,
Author = {S. Y. Al-ramadan and G. A. Johnson and L. A. Jaeger and S.
P. Brinsko and R. C. Burghardt},
Title = {Distribution of integrin subunits, MUC-1, and osteopontin in
equine uterine epithelium and conceptuses during early
pregnancy.},
Journal = {Biology Of Reproduction},
Volume = {66},
Pages = {323 -- 323},
Year = {2002},
Key = {Al-ramadan02}
}
@booklet{Helson80a,
Author = {L. Helson and A. Majeranowski and C. Helson and M. Schwartz and G. A. Johnson and J. Nisselbaum},
Title = {Dopa decarboxylase (ddc) and tryptophan decarboxylase(tdc)
in neuroectodermal tumors - 2 separate enzymes},
Journal = {Proceedings Of The American Association For Cancer
Research},
Volume = {21},
Number = {MAR},
Pages = {322 -- 322},
Year = {1980},
Key = {Helson80a}
}
@booklet{Helson80,
Author = {L. Helson and G. A. Johnson and R. Smith},
Title = {Dopa metabolism in neuro-blastoma},
Journal = {Medical And Pediatric Oncology},
Volume = {8},
Number = {4},
Pages = {317 -- 322},
Year = {1980},
Key = {Helson80}
}
@article{fds174104,
Author = {L Helson and GA Johnson and R Smith},
Title = {DOPA metabolism in neuroblastoma.},
Journal = {Medical and pediatric oncology},
Volume = {8},
Number = {4},
Pages = {317-22},
Year = {1980},
ISSN = {0098-1532},
Keywords = {Adolescent • Bone Neoplasms • Brain Neoplasms
• Child • Dihydroxyphenylalanine • False
Negative Reactions • Female • Humans •
Leukemia • Lymphoma • Neoplasm Recurrence, Local
• Neuroblastoma • Time Factors •
Vanilmandelic Acid • blood • cerebrospinal fluid
• metabolism • metabolism* •
urine},
Abstract = {Blood plasma samples from 60 neuroblastoma patients prior
to, during, and following treatment were studied for their
content of circulating DOPA using a radioenzymatic assay.
Normal values were established from children who were
tumor-free or had other nonneurogenic tumors. The highest
plasma DOPA concentration in tumor-free or nonneuroblastoma
controls was 5.3 ng/ml with a mean of 2.15 ng/ml. Most
neuroblastoma patients (28/31) with active disease had DOPA
values above this level. Only one out of 30 "successfully"
treated patients without evidence of disease was encountered
with an abnormally high level. In treated patients, elevated
values forewarned of impending clinical recurrence or
persistent tumor. Cerebrospinal fluid DOPA levels in one
patient with cerebral neuroblastoma were extraordinarily
high and suggests that this assay may prove useful to
distinguish neuroblastoma from other central neuroectodermal
or metastatic tumors. Plasma DOPA appears to be a reliable
predictive and diagnostic test for neuroblastoma.},
Language = {eng},
Key = {fds174104}
}
@article{fds174247,
Author = {PF Von Voigtlander and SJ Boukma and GA Johnson},
Title = {Dopaminergic denervation supersensitivity and dopamine
stimulated adenyl cyclase activity.},
Journal = {Neuropharmacology},
Volume = {12},
Number = {11},
Pages = {1081-6},
Year = {1973},
Month = {November},
ISSN = {0028-3908},
Keywords = {Adenylate Cyclase • Animals • Apomorphine •
Corpus Striatum • Cyclic AMP • Dopamine •
Dose-Response Relationship, Drug • Hydroxydopamines
• Male • Methyltyrosines • Mice • Nerve
Endings • Receptors, Drug • Stimulation, Chemical
• Sympathectomy • biosynthesis • drug effects
• enzymology • metabolism • metabolism*
• pharmacology • pharmacology* •
physiology},
Language = {eng},
Key = {fds174247}
}
@article{fds268734,
Author = {Bhavane, R and Badea, C and Ghaghada, KB and Clark, D and Vela, D and Moturu, A and Annapragada, A and Johnson, GA and Willerson, JT and Annapragada, A},
Title = {Dual-energy computed tomography imaging of atherosclerotic
plaques in a mouse model using a liposomal-iodine
nanoparticle contrast agent.},
Journal = {Circ Cardiovasc Imaging},
Volume = {6},
Number = {2},
Pages = {285-294},
Year = {2013},
Month = {March},
ISSN = {1941-9651},
url = {http://dx.doi.org/10.1161/CIRCIMAGING.112.000119},
Abstract = {BACKGROUND: The accumulation of macrophages in inflamed
atherosclerotic plaques has long been recognized. In an
attempt to develop an imaging agent for detection of
vulnerable plaques, we evaluated the feasibility of a
liposomal-iodine nanoparticle contrast agent for computed
tomography imaging of macrophage-rich atherosclerotic
plaques in a mouse model. METHODS AND RESULTS:
Liposomal-iodine formulations varying in particle size and
polyethylene glycol coating were fabricated and shown to
stably encapsulate the iodine compound. In vitro uptake
studies using optical and computed tomography imaging in the
RAW 264.7 macrophage cell line identified the formulation
that promoted maximal uptake. Dual-energy computed
tomography imaging using this formulation in apolipoprotein
E-deficient (ApoE(-/-)) mice (n=8) and control C57BL/6 mice
(n=6) followed by spectral decomposition of the dual-energy
images enabled imaging of the liposomes localized in the
plaque. Imaging cytometry confirmed the presence of
liposomes in the plaque and their colocalization with a
small fraction (≈2%) of the macrophages in the plaque.
CONCLUSIONS: The results demonstrate the feasibility of
imaging macrophage-rich atherosclerotic plaques using a
liposomal-iodine nanoparticle contrast agent and dual-energy
computed tomography.},
Doi = {10.1161/CIRCIMAGING.112.000119},
Key = {fds268734}
}
@article{fds268806,
Author = {Moding, EJ and Clark, DP and Qi, Y and Li, Y and Ma, Y and Ghaghada, K and Johnson, GA and Kirsch, DG and Badea, CT},
Title = {Dual-energy micro-computed tomography imaging of
radiation-induced vascular changes in primary mouse
sarcomas.},
Journal = {Int J Radiat Oncol Biol Phys},
Volume = {85},
Number = {5},
Pages = {1353-1359},
Year = {2013},
Month = {April},
url = {http://www.ncbi.nlm.nih.gov/pubmed/23122984},
Abstract = {PURPOSE: To evaluate the effects of radiation therapy on
primary tumor vasculature using dual-energy (DE)
micro-computed tomography (micro-CT). METHODS AND MATERIALS:
Primary sarcomas were generated with mutant Kras and p53.
Unirradiated tumors were compared with tumors irradiated
with 20 Gy. A liposomal-iodinated contrast agent was
administered 1 day after treatment, and mice were imaged
immediately after injection (day 1) and 3 days later (day 4)
with DE micro-CT. CT-derived tumor sizes were used to assess
tumor growth. After DE decomposition, iodine maps were used
to assess tumor fractional blood volume (FBV) at day 1 and
tumor vascular permeability at day 4. For comparison, tumor
vascularity and vascular permeability were also evaluated
histologically by use of CD31 immunofluorescence and
fluorescently-labeled dextrans. RESULTS: Radiation treatment
significantly decreased tumor growth from day 1 to day 4
(P<.05). There was a positive correlation between CT
measurement of tumor FBV on day 1 and extravasated iodine on
day 4 with microvascular density (MVD) on day 4 (R(2)=0.53)
and dextran accumulation (R(2)=0.63) on day 4, respectively.
Despite no change in MVD measured by histology, tumor FBV
significantly increased after irradiation as measured by DE
micro-CT (0.070 vs 0.091, P<.05). Both dextran and
liposomal-iodine accumulation in tumors increased
significantly after irradiation, with dextran fractional
area increasing 5.2-fold and liposomal-iodine concentration
increasing 4.0-fold. CONCLUSIONS: DE micro-CT is an
effective tool for noninvasive assessment of vascular
changes in primary tumors. Tumor blood volume and vascular
permeability increased after a single therapeutic dose of
radiation treatment.},
Doi = {10.1016/j.ijrobp.2012.09.027},
Key = {fds268806}
}
@article{fds268785,
Author = {Badea, CT and Johnston, SM and Qi, Y and Ghaghada, K and Johnson,
GA},
Title = {Dual-energy micro-CT imaging for differentiation of iodine-
and gold-based nanoparticles},
Journal = {Progress in Biomedical Optics and Imaging - Proceedings of
SPIE},
Volume = {7961},
Publisher = {SPIE},
Year = {2011},
Month = {May},
ISBN = {9780819485038},
ISSN = {1605-7422},
url = {http://dx.doi.org/10.1117/12.878043},
Abstract = {Spectral CT imaging is expected to play a major role in the
diagnostic arena as it provides material decomposition on an
elemental basis. One fascinating possibility is the ability
to discriminate multiple contrast agents targeting different
biological sites. We investigate the feasibility of dual
energy micro-CT for discrimination of iodine (I) and gold
(Au) contrast agents when simultaneously present in the
body. Simulations and experiments were performed to measure
the CT enhancement for I and Au over a range of voltages
from 40-to-150 kVp using a dual source micro-CT system. The
selected voltages for dual energy micro-CT imaging of Au and
I were 40 kVp and 80 kVp. On a massconcentration basis, the
relative average enhancement of Au to I was 2.75 at 40 kVp
and 1.58 at 80 kVp. We have demonstrated the method in a
preclinical model of colon cancer to differentiate vascular
architecture and extravasation. The concentration maps of Au
and I allow quantitative measure of the bio-distribution of
both agents. In conclusion, dual energy micro-CT can be used
to discriminate probes containing I and Au with immediate
impact in pre-clinical research. © 2011
SPIE.},
Doi = {10.1117/12.878043},
Key = {fds268785}
}
@article{fds268714,
Author = {Badea, CT and Befera, N and Clark, D and Qi, Y and Johnson,
GA},
Title = {Dual-energy micro-CT imaging of pulmonary airway
obstruction: Correlation with micro-SPECT},
Journal = {Progress in Biomedical Optics and Imaging - Proceedings of
SPIE},
Volume = {9038},
Publisher = {SPIE},
Year = {2014},
Month = {January},
ISBN = {9780819498311},
ISSN = {1605-7422},
url = {http://dx.doi.org/10.1117/12.2043094},
Abstract = {To match recent clinical dual energy (DE) CT studies
focusing on the lung, similar developments for DE micro-CT
of the rodent lung are required. Our group has been actively
engaged in designing pulmonary gating techniques for micro-
CT, and has also introduced the first DE micro-CT imaging
method of the rodent lung. The aim of this study was to
assess the feasibility of DE micro-CT imaging for the
evaluation of airway obstruction in mice, and to compare the
method with micro single photon emission computed tomography
(micro-SPECT) using technetium-99m labeled macroaggregated
albumin (99mTc-MAA). The results suggest that the induced
pulmonary airway obstruction causes either atelectasis, or
air-trapping similar to asthma or chronic bronchitis.
Atelectasis could only be detected at early time points in
DE micro-CT images, and is associated with a large increase
in blood fraction and decrease in air fraction. Air trapping
had an opposite effect with larger air fraction and
decreased blood fraction shown by DE micro-CT. The decrease
in perfusion to the hypoventilated lung (hypoxic
vasoconstriction) is also seen in micro-SPECT. The proposed
DE micro-CT technique for imaging localized airway
obstruction performed well in our evaluation, and provides a
higher resolution compared to micro-SPECT. Both DE micro-CT
and micro-SPECT provide critical, quantitative lung
biomarkers for image-based anatomical and functional
information in the small animal. The methods are readily
linked to clinical methods allowing direct comparison of
preclinical and clinical results. © 2014
SPIE.},
Doi = {10.1117/12.2043094},
Key = {fds268714}
}
@article{fds268698,
Author = {Subashi, E and Qi, Y and Johnson, GA},
Title = {Dynamic contrast-enhanced MR microscopy identifies regions
of therapeutic response in a preclinical model of colorectal
adenocarcinoma.},
Journal = {Med Phys},
Volume = {42},
Number = {5},
Pages = {2482-2488},
Year = {2015},
Month = {May},
ISSN = {0094-2405},
url = {http://dx.doi.org/10.1118/1.4917525},
Abstract = {PURPOSE: A typical dynamic contrast-enhanced (DCE)-MRI study
often compares the derived pharmacokinetic parameters on
manually selected tumor regions or over the entire tumor
volume. These measurements include domains where the
interpretation of the biomarkers may be unclear (such as in
necrotic areas). Here, the authors describe a technique for
increasing the sensitivity and specificity of DCE-MRI by
identifying tumor regions with a variable response to
therapy. METHODS: Two cohorts (n = 8/group) of nu/nu mice
with LS-174T implanted in the mammary fat pad were imaged at
five time points over four weeks. The treatment/control
group received bevacizumab/saline at a dose of 5 mg/kg or 5
ml/kg twice weekly; imaging experiments were performed
weekly. MR images were acquired at an isotropic resolution
of 156 μm(3)(2.4 nl) and with a sampling rate of 9.9 s. The
histogram of the time-to-peak (TTP) was used to identify two
(fast- and slow-enhancing) regions based on a threshold of
TTP = 1000 s. The regions were correlated with histology,
and the effect of therapy was locally examined. RESULTS:
Tumors in the treatment group had a significantly longer
doubling time. The regions defined by thresholding the TTP
histogram identified two distinct domains correlating
significantly with tumor permeability and microvessel
density. In the fast-enhancing region, the mean permeability
constant (K(trans)) was significantly lower in the treatment
group at day 9; in the slow-enhancing region, K(trans) was
not different between the control and treatment groups. At
day 9, the relative volume of the fast-enhancing region was
significantly lower in the treatment group, while that of
the slow-enhancing region was significantly higher.
CONCLUSIONS: Two regions with distinct kinetic parameters
were identified based on the histogram of TTP. The effect of
bevacizumab, as measured by a decrease in K(trans), was
confined to one of these regions. High spatiotemporal
resolution MR studies may contribute unique insights into
the response of the tumor microenvironment to
therapy.},
Doi = {10.1118/1.4917525},
Key = {fds268698}
}
@article{fds341984,
Author = {Privratsky, JR and Wang, N and Qi, Y and Ren, J and Morris, BT and Hunting,
JC and Johnson, GA and Crowley, SD},
Title = {Dynamic contrast-enhanced MRI promotes early detection of
toxin-induced acute kidney injury.},
Journal = {Am J Physiol Renal Physiol},
Volume = {316},
Number = {2},
Pages = {F351-F359},
Year = {2019},
Month = {February},
url = {http://dx.doi.org/10.1152/ajprenal.00416.2018},
Abstract = {Acute kidney injury (AKI) is a common cause of morbidity and
mortality in hospitalized patients. Nevertheless, there is
limited ability to diagnose AKI in its earliest stages
through the collection of structural and functional
information. Magnetic resonance imaging (MRI) is
increasingly being used to provide structural and functional
data that characterize the injured kidney. Dynamic
contrast-enhanced (DCE) MRI is an imaging modality with
robust spatial and temporal resolution; however, its ability
to detect changes in kidney function following AKI has not
been determined. We hypothesized that DCE MRI would detect a
prolongation in contrast transit time following
toxin-induced AKI earlier than commonly used serum and
tissue biomarkers. To test our hypothesis, we injected mice
with either vehicle or cisplatin (30 mg/kg) and performed
DCE MRI at multiple time points. We found that commonly used
kidney injury biomarkers, including creatinine, blood urea
nitrogen, and neutrophil gelatinase-associated lipocalin,
did not rise until day 2 following cisplatin. Tissue levels
of the proinflammatory cytokines and chemokines, tumor
necrosis factor-α, interleukin (IL)-1β, IL-1α, IL-6, C-C
motif chemokine ligand 2, and C-X-C motif chemokine ligand 2
similarly did not upregulate until day 2 following
cisplatin. However, the time to peak intensity of contrast
in the renal collecting system was already prolonged at day
1 following cisplatin compared with vehicle-treated mice.
This intensity change mirrored changes in kidney injury as
measured by histological analysis and in transporter
expression in the proximal tubule. Taken together, DCE MRI
is a promising preclinical imaging modality that is useful
for assessing functional capacity of the kidney in the
earliest stages following AKI.},
Doi = {10.1152/ajprenal.00416.2018},
Key = {fds341984}
}
@article{fds292754,
Author = {Xie, L and Layton, AT and Wang, N and Larson, PEZ and Zhang, JL and Lee,
VS and Liu, C and Johnson, GA},
Title = {Dynamic contrast-enhanced quantitative susceptibility
mapping with ultrashort echo time MRI for evaluating renal
function.},
Journal = {Am J Physiol Renal Physiol},
Volume = {310},
Number = {2},
Pages = {F174-F182},
Year = {2016},
Month = {January},
ISSN = {1931-857X},
url = {http://dx.doi.org/10.1152/ajprenal.00351.2015},
Abstract = {Dynamic contrast-enhanced (DCE) MRI can provide key insight
into renal function. DCE MRI is typically achieved through
an injection of a gadolinium (Gd)-based contrast agent,
which has desirable T1 quenching and tracer kinetics.
However, significant T2* blooming effects and signal voids
can arise when Gd becomes very concentrated, especially in
the renal medulla and pelvis. One MRI sequence designed to
alleviate T2* effects is the ultrashort echo time (UTE)
sequence. In the present study, we observed T2* blooming in
the inner medulla of the mouse kidney, despite using UTE at
an echo time of 20 microseconds and a low dose of 0.03
mmol/kg Gd. We applied quantitative susceptibility mapping
(QSM) and resolved the signal void into a positive
susceptibility signal. The susceptibility values [in parts
per million (ppm)] were converted into molar concentrations
of Gd using a calibration curve. We determined the
concentrating mechanism (referred to as the concentrating
index) as a ratio of maximum Gd concentration in the inner
medulla to the renal artery. The concentrating index was
assessed longitudinally over a 17-wk course (3, 5, 7, 9, 13,
17 wk of age). We conclude that the UTE-based DCE method is
limited in resolving extreme T2* content caused by the
kidney's strong concentrating mechanism. QSM was able to
resolve and confirm the source of the blooming effect to be
the large positive susceptibility of concentrated Gd. UTE
with QSM can complement traditional magnitude UTE and offer
a powerful tool to study renal pathophysiology.},
Doi = {10.1152/ajprenal.00351.2015},
Key = {fds292754}
}
@article{fds132914,
Author = {BT Chen and GA Johnson},
Title = {Dynamic lung morphology of methacholine-induced
heterogeneous bronchoconstriction.},
Journal = {Magnetic resonance in medicine : official journal of the
Society of Magnetic Resonance in Medicine / Society of
Magnetic Resonance in Medicine, United States},
Volume = {52},
Number = {5},
Pages = {1080-6},
Year = {2004},
Month = {November},
ISSN = {0740-3194},
Keywords = {Animals • Bronchoconstriction* • Female •
Helium • Injections, Intravenous • Isotopes •
Magnetic Resonance Imaging • Methacholine Chloride
• Rats • Rats, Sprague-Dawley • Respiratory
System • administration & dosage • diagnostic use
• drug effects* • methods* •
pharmacology*},
Abstract = {Hyperpolarized (HP) 3helium (3He) dynamic MRI was used to
investigate airway response in rats following intravenous
(i.v.) bolus administration of a contractile agent,
methacholine (MCh). The method provides direct visualization
of the ventilated regions within the lung. Heterogeneous
bronchoconstriction following the i.v. MCh injection was
evident using this technique. These 3He dynamic lung images
revealed that the inspired fresh air was shunted to the
less-constricted regions after the MCh challenge in a
similar manner as described by Laplace's relationship for
the stability between adjacent alveoli. The airways in the
more-constricted regions became nearly closed, resulting in
air trapping, while the airways in the less-constricted
regions remained effectively open, leading to overinflation.
These data suggest a lung model of airway constriction
partitioned into ventilated and nonventilated regions. These
nonventilated regions are heterogeneously distributed in the
lung and this distribution cannot be deduced from
spirometric measurement of the whole lung. We demonstrate
that a combination of functional 3He images and anatomical
1H images provide an effective method to diagnose regional
lung abnormalities in rats.},
Key = {fds132914}
}
@booklet{Chen04,
Author = {Chen, BT and Johnson, GA},
Title = {Dynamic lung morphology of methacholine-induced
heterogeneous bronchoconstriction.},
Journal = {Magn Reson Med},
Volume = {52},
Number = {5},
Pages = {1080-1086},
Year = {2004},
Month = {November},
ISSN = {0740-3194},
url = {http://www.ncbi.nlm.nih.gov/pubmed/15508158},
Abstract = {Hyperpolarized (HP) 3helium (3He) dynamic MRI was used to
investigate airway response in rats following intravenous
(i.v.) bolus administration of a contractile agent,
methacholine (MCh). The method provides direct visualization
of the ventilated regions within the lung. Heterogeneous
bronchoconstriction following the i.v. MCh injection was
evident using this technique. These 3He dynamic lung images
revealed that the inspired fresh air was shunted to the
less-constricted regions after the MCh challenge in a
similar manner as described by Laplace's relationship for
the stability between adjacent alveoli. The airways in the
more-constricted regions became nearly closed, resulting in
air trapping, while the airways in the less-constricted
regions remained effectively open, leading to overinflation.
These data suggest a lung model of airway constriction
partitioned into ventilated and nonventilated regions. These
nonventilated regions are heterogeneously distributed in the
lung and this distribution cannot be deduced from
spirometric measurement of the whole lung. We demonstrate
that a combination of functional 3He images and anatomical
1H images provide an effective method to diagnose regional
lung abnormalities in rats.},
Doi = {10.1002/mrm.20251},
Key = {Chen04}
}
@article{fds132844,
Author = {GA Johnson and G Cates and XJ Chen and GP Cofer and B Driehuys and W
Happer, LW Hedlund and B Saam, MD Shattuck and J
Swartz},
Title = {Dynamics of magnetization in hyperpolarized gas MRI of the
lung.},
Journal = {Magnetic resonance in medicine : official journal of the
Society of Magnetic Resonance in Medicine / Society of
Magnetic Resonance in Medicine, UNITED STATES},
Volume = {38},
Number = {1},
Pages = {66-71},
Year = {1997},
Month = {July},
ISSN = {0740-3194},
Keywords = {Animals • Guinea Pigs • Helium • Isotopes
• Lung • Magnetic Resonance Imaging • Male
• Respiration • anatomy & histology* •
diagnostic use* • methods*},
Abstract = {The magnetization in hyperpolarized gas (HP) MRI is
generated by laser polarization that is independent of the
magnet and imaging process. As a consequence, there is no
equilibrium magnetization during the image acquisition. The
competing processes of gas inflow and depolarization of the
spins lead to large changes in signal as one samples
k-space. A model is developed of dynamic changes in
polarization of hyperpolarized 3He during infusion and in
vivo imaging of the lung and verified experimentally in a
live guinea pig. Projection encoding is used to measure the
view-to-view variation with temporal resolution < 4 ms.
Large excitation angles effectively sample the magnetization
in the early stages of inflow, highlighting larger airways,
while smaller excitation angles produce images of the more
distal spaces. The work provides a basis for pulse sequences
designed to effectively exploit HP MRI in the
lung.},
Key = {fds132844}
}
@booklet{Johnson97b,
Author = {Johnson, GA and Cates, G and Chen, XJ and Cofer, GP and Driehuys, B and Happer, W and Hedlund, LW and Saam, B and Shattuck, MD and Swartz,
J},
Title = {Dynamics of magnetization in hyperpolarized gas MRI of the
lung.},
Journal = {Magn Reson Med},
Volume = {38},
Number = {1},
Pages = {66-71},
Year = {1997},
Month = {July},
ISSN = {0740-3194},
url = {http://www.ncbi.nlm.nih.gov/pubmed/9211381},
Abstract = {The magnetization in hyperpolarized gas (HP) MRI is
generated by laser polarization that is independent of the
magnet and imaging process. As a consequence, there is no
equilibrium magnetization during the image acquisition. The
competing processes of gas inflow and depolarization of the
spins lead to large changes in signal as one samples
k-space. A model is developed of dynamic changes in
polarization of hyperpolarized 3He during infusion and in
vivo imaging of the lung and verified experimentally in a
live guinea pig. Projection encoding is used to measure the
view-to-view variation with temporal resolution < 4 ms.
Large excitation angles effectively sample the magnetization
in the early stages of inflow, highlighting larger airways,
while smaller excitation angles produce images of the more
distal spaces. The work provides a basis for pulse sequences
designed to effectively exploit HP MRI in the
lung.},
Doi = {10.1002/mrm.1910380111},
Key = {Johnson97b}
}
@booklet{Kodali03,
Author = {K. Kodali and C. M. Davitt and G. A. Johnson and T. L.
Ott},
Title = {Dynamin family member and antiviral protein, Mx,
co-localizes with autocrine motility factor receptor in an
ovine uterine lumenal epithelial cell line.},
Journal = {Biology Of Reproduction},
Volume = {68},
Pages = {181 -- 181},
Year = {2003},
Key = {Kodali03}
}
@booklet{Lindquist98,
Author = {J. L. Lindquist and J. A. Dieleman and D. A. Mortensen and G. A. Johnson and D. Y. Wyse-pester},
Title = {Economic importance of managing spatially heterogeneous weed
populations},
Journal = {Weed Technology},
Volume = {12},
Number = {1},
Pages = {7 -- 13},
Year = {1998},
Key = {Lindquist98}
}
@article{fds174258,
Author = {GA Johnson and RA Lahti and TL Lemke and RV Heinzelman},
Title = {Effect of alpha-methyl-5-hydroxytryptophan ethyl ester upon
tissue norepinephrine levels in rats and
mice.},
Journal = {Biochemical pharmacology},
Volume = {18},
Number = {7},
Pages = {1593-600},
Year = {1969},
Month = {July},
ISSN = {0006-2952},
Keywords = {5-Hydroxytryptophan • Adrenal Glands • Animals
• Brain • Brain Chemistry • Esters •
Mice • Mixed Function Oxygenases • Myocardium
• Norepinephrine • Rats • Time Factors •
Tyrosine • administration & dosage • analysis
• analysis* • enzymology • metabolism •
pharmacology • pharmacology*},
Language = {eng},
Key = {fds174258}
}
@booklet{Baker85,
Author = {C. A. Baker and D. W. Harris and G. A. Johnson},
Title = {Effect of atriopeptin-ii on cyclic-nucleotide production in
tissue-culture cells},
Journal = {Federation Proceedings},
Volume = {44},
Number = {4},
Pages = {1108 -- 1108},
Year = {1985},
Key = {Baker85}
}
@article{fds174178,
Author = {SA Warnke and SY Chen and DL Wyse and GA Johnson and PM
Porter},
Title = {Effect of Rotation Crops on Heterodera glycines Population
Density in a Greenhouse Screening Study.},
Journal = {Journal of nematology},
Volume = {38},
Number = {3},
Pages = {391-8},
Year = {2006},
Month = {September},
ISSN = {0022-300X},
Abstract = {Crop rotation is a common means of reducing pathogen
populations in soil. Several rotation crops have been shown
to reduce soybean cyst nematode (Heterodera glycines)
populations, but a comprehensive study of the optimal crops
is needed. A greenhouse study was conducted to determine the
effect of growth and decomposition of 46 crops on population
density of H. glycines. Crops were sown in soil infested
with H. glycines. Plants were maintained until 75 days after
planting, when the soil was mixed, a sample of the soil
removed to determine egg density, and shoots and roots
chopped and mixed into the soil. After 56 days, soil samples
were again taken for egg counts, and a susceptible soybean
('Sturdy') was planted in the soil as a bioassay to
determine egg viability. Sunn hemp (Crotalaria juncea),
forage pea (Pisum sativum), lab-lab bean (Lablab purpureus),
Illinois bundleflower (Desman-thus illinoensis), and alfalfa
(Medicago sativa) generally resulted in smaller egg
population density in soil or number of cysts formed on
soybean in the bioassay than the fallow control. Sunn hemp
most consistently showed the lowest numbers of eggs and
cysts. As a group, legumes resulted in lower egg population
densities than monocots, Brassica species, and other
dicots.},
Language = {eng},
Key = {fds174178}
}
@booklet{Johnson02b,
Author = {G. A. Johnson and T. R. Hoverstad},
Title = {Effect of row spacing and herbicide application timing on
weed control and grain yield in corn (Zea
mays)},
Journal = {Weed Technology},
Volume = {16},
Number = {3},
Pages = {548 -- 553},
Year = {2002},
Key = {Johnson02b}
}
@booklet{Stahl99,
Author = {L. A. B. Stahl and G. A. Johnson and D. L. Wyse and D. D.
Buhler and J. L. Gunsolus},
Title = {Effect of tillage on timing of Setaria spp. emergence and
growth},
Journal = {Weed Science},
Volume = {47},
Number = {5},
Pages = {563 -- 570},
Year = {1999},
Key = {Stahl99}
}
@article{fds132769,
Author = {GA Johnson and CE Ravin},
Title = {Effect of vertical cassette holder design and construction
on scatter-to-primary radiation ratios.},
Journal = {Radiology, UNITED STATES},
Volume = {138},
Number = {2},
Pages = {461-4},
Year = {1981},
Month = {February},
ISSN = {0033-8419},
Keywords = {Humans • Radiography • Scattering, Radiation*
• Technology, Radiologic • instrumentation*},
Abstract = {This paper analyzes the contribution to scattered radiation
at the film plane by individual components of a conventional
wall-mounted cassette holder. With grid technique, scatter
from the front panel of the cassette holder is effectively
reduced, but considerable scatter reaches the film plane
from the automatic exposure control pickup interposed
between the grid and the film cassette. With air-gap
technique (grid removed), the front panel of the cassette
holder and the automatic exposure control pickup contribute
equally to increased scattered radiation at the film plane.
The contribution to the total scatter from the air-gap
positioning plate is less significant.},
Key = {fds132769}
}
@booklet{Johnson81a,
Author = {Johnson, GA and Ravin, CE},
Title = {Effect of vertical cassette holder design and construction
on scatter-to-primary radiation ratios.},
Journal = {Radiology},
Volume = {138},
Number = {2},
Pages = {461-464},
Year = {1981},
Month = {February},
ISSN = {0033-8419},
url = {http://www.ncbi.nlm.nih.gov/pubmed/7455131},
Abstract = {This paper analyzes the contribution to scattered radiation
at the film plane by individual components of a conventional
wall-mounted cassette holder. With grid technique, scatter
from the front panel of the cassette holder is effectively
reduced, but considerable scatter reaches the film plane
from the automatic exposure control pickup interposed
between the grid and the film cassette. With air-gap
technique (grid removed), the front panel of the cassette
holder and the automatic exposure control pickup contribute
equally to increased scattered radiation at the film plane.
The contribution to the total scatter from the air-gap
positioning plate is less significant.},
Doi = {10.1148/radiology.138.2.7455131},
Key = {Johnson81a}
}
@article{fds325755,
Author = {O'Leary-Moore, SK and Godin, EA and Parnell, SE and Dehart, DB and Ament, JJ and Johnson, GA and Styner, M and Sulik,
KK},
Title = {EFFECTS OF ACUTE GESTATIONAL DAY 10 ETHANOL EXPOSURE ON THE
DEVELOPING MOUSE BRAIN: A HIGH-RESOLUTION MAGNETIC RESONANCE
MICROSCOPY STUDY},
Journal = {ALCOHOLISM-CLINICAL AND EXPERIMENTAL RESEARCH},
Volume = {33},
Number = {6},
Pages = {132A-132A},
Year = {2009},
Key = {fds325755}
}
@article{8407765,
Author = {Mai, W. and Badea, C.T. and Wheeler, C.T. and Hedlund, L.W. and Johnson, G.A.},
Title = {Effects of breathing and cardiac motion on spatial
resolution in the microscopic imaging of
rodents},
Journal = {Magn. Reson. Med. (USA)},
Volume = {53},
Number = {4},
Pages = {858 - 65},
Year = {2005},
url = {http://dx.doi.org/10.1002/mrm.20400},
Keywords = {biomedical MRI;cardiovascular system;diagnostic
radiography;pneumodynamics;surgery;},
Abstract = {One can acquire high-resolution pulmonary and cardiac images
in live rodents with MR microscopy by synchronizing the
image acquisition to the breathing cycle across multiple
breaths, and gating to the cardiac cycle. The precision with
which one can synchronize image acquisition to the motion
defines the ultimate resolution limit that can be attained
in such studies. The present work was performed to evaluate
how reliably the pulmonary and cardiac structures return to
the same position from breath to breath and beat to beat
across the prolonged period required for MR microscopy.
Radiopaque beads were surgically glued to the abdominal
surface of the diaphragm and on the cardiac ventricles of
anesthetized, mechanically ventilated rats. We evaluated the
range of motion for the beads (relative to a reference
vertebral bead) using digital microradiography with two
specific biological gating methods: 1) ventilation
synchronous acquisition, and 2) both ventilation synchronous
and cardiac-gated acquisitions. The standard deviation (SD)
of the displacement was μm100 μm, which is comparable
to the resolution limit for in vivo MRI imposed by
signal-to-noise ratio (SNR) constraints. With careful
control of motion, its impact on resolution can be limited.
This work provides the first quantitative measure of the
motion-imposed resolution limits for in vivo
imaging},
Key = {8407765}
}
@article{fds268882,
Author = {Maï, W and Badea, CT and Wheeler, CT and Hedlund, LW and Johnson,
GA},
Title = {Effects of breathing and cardiac motion on spatial
resolution in the microscopic imaging of
rodents.},
Journal = {Magn Reson Med},
Volume = {53},
Number = {4},
Pages = {858-865},
Year = {2005},
Month = {April},
ISSN = {0740-3194},
url = {http://www.ncbi.nlm.nih.gov/pubmed/15799053},
Keywords = {Animals • Cardiovascular Physiology* • Diaphragm
• Magnetic Resonance Imaging • Male •
Myocardial Contraction • Rats • Rats, Inbred F344
• Reproducibility of Results • Tungsten •
methods* • physiology* • radiography},
Abstract = {One can acquire high-resolution pulmonary and cardiac images
in live rodents with MR microscopy by synchronizing the
image acquisition to the breathing cycle across multiple
breaths, and gating to the cardiac cycle. The precision with
which one can synchronize image acquisition to the motion
defines the ultimate resolution limit that can be attained
in such studies. The present work was performed to evaluate
how reliably the pulmonary and cardiac structures return to
the same position from breath to breath and beat to beat
across the prolonged period required for MR microscopy.
Radiopaque beads were surgically glued to the abdominal
surface of the diaphragm and on the cardiac ventricles of
anesthetized, mechanically ventilated rats. We evaluated the
range of motion for the beads (relative to a reference
vertebral bead) using digital microradiography with two
specific biological gating methods: 1) ventilation
synchronous acquisition, and 2) both ventilation synchronous
and cardiac-gated acquisitions. The standard deviation (SD)
of the displacement was < or =100 microm, which is
comparable to the resolution limit for in vivo MRI imposed
by signal-to-noise ratio (SNR) constraints. With careful
control of motion, its impact on resolution can be limited.
This work provides the first quantitative measure of the
motion-imposed resolution limits for in vivo
imaging.},
Doi = {10.1002/mrm.20400},
Key = {fds268882}
}
@article{fds268777,
Author = {Mai, W and Badea, CT and Wheeler, CT and Hedlund, LW and Johnson,
GA},
Title = {Effects of breathing motion on the spatial resolution in
microscopic imaging techniques of rodents},
Journal = {2004 2nd IEEE International Symposium on Biomedical Imaging:
Macro to Nano},
Volume = {1},
Pages = {472-475},
Year = {2004},
Month = {December},
ISBN = {0780383885},
Abstract = {Magnetic resonance microscopy is capable of producing
high-resolution pulmonary images in live rodents by
synchronizing the image acquisition across multiple breaths.
The precision with which one can control motion will
probably define the resolution limit that can be attained in
such studies. This work was performed to evaluate how
reliably the respiratory structures return to the same
position from breath to breath each time data are acquired.
Radio-opaque beads were surgically glued on the diaphragm of
anesthetized, mechanically ventilated rats. Their range of
motion (relative to a reference vertebral bead) was
evaluated using digital micro-radiography with two specific
biological pulse sequences: (1) ventilation synchronous
acquisition, and (2) both ventilation synchronous and
cardiac gated acquisition. The standard deviation of the
displacement was on the order of, or less than 100 microns,
which is comparable to the resolution limit for in vivo
magnetic resonance imaging imposed by signal to noise
constraints. With careful control of motion, its impact on
resolution can be limited. This work provides the first
quantitative measure of the motion imposed resolution limits
for in vivo imaging. ©2004 IEEE.},
Key = {fds268777}
}
@article{fds325752,
Author = {Parnell, SE and Holloway, HT and Paniagua, B and Oguz, I and Styner, MA and Johnson, GA and Sulik, KK},
Title = {EFFECTS OF CHRONIC EARLY GESTATIONAL ETHANOL EXPOSURE ON THE
DEVELOPING BRAIN: A MAGNETIC RESONANCE MICROSCOPY
STUDY},
Journal = {ALCOHOLISM-CLINICAL AND EXPERIMENTAL RESEARCH},
Volume = {36},
Pages = {279A-279A},
Year = {2012},
Key = {fds325752}
}
@article{fds132873,
Author = {RH Sherrier and C Chiles and WE Wilkinson and GA Johnson and CE
Ravin},
Title = {Effects of image processing on nodule detection rates in
digitized chest radiographs: ROC study of observer
performance.},
Journal = {Radiology, UNITED STATES},
Volume = {166},
Number = {2},
Pages = {447-50},
Year = {1988},
Month = {February},
ISSN = {0033-8419},
Keywords = {Coin Lesion, Pulmonary • Filtration • Humans
• Lung Neoplasms • ROC Curve* • Radiographic
Image Enhancement • methods • methods* •
radiography*},
Abstract = {To evaluate the effects of image processing in digitized
chest radiographs when high-resolution images are used, an
examination was done in which the detection of pulmonary
nodules in unprocessed digitized chest radiographs was
compared with that in images that had undergone processing
with two methods, adaptive filtration and histogram
equalization. The processing techniques have been optimized
in previous work to selectively enhance the retrocardiac and
subdiaphragmatic areas without significant alteration of
detail in the lung. Eight observers were shown 150 test
radiographs (50 unprocessed, 50 processed with adaptive
filtration, 50 processed with histogram equalization)
containing 150 nodules. The results indicate a statistically
significant (P less than .03) difference, with highest
observer performance in the chest radiographs processed with
adaptive filtration (median area under ROC curve = 0.78),
compared with unprocessed images (median = 0.68) and chest
radiographs processed with histogram equalization (median =
0.62). Performance in the lung was not significantly
different. Adaptive filtration applied to selectively
enhance underexposed areas of film images may improve nodule
detection. Histogram equalization provided no improvement in
performance.},
Key = {fds132873}
}
@booklet{Sherrier88a,
Author = {Sherrier, RH and Chiles, C and Wilkinson, WE and Johnson, GA and Ravin,
CE},
Title = {Effects of image processing on nodule detection rates in
digitized chest radiographs: ROC study of observer
performance.},
Journal = {Radiology},
Volume = {166},
Number = {2},
Pages = {447-450},
Year = {1988},
Month = {February},
ISSN = {0033-8419},
url = {http://www.ncbi.nlm.nih.gov/pubmed/3336719},
Abstract = {To evaluate the effects of image processing in digitized
chest radiographs when high-resolution images are used, an
examination was done in which the detection of pulmonary
nodules in unprocessed digitized chest radiographs was
compared with that in images that had undergone processing
with two methods, adaptive filtration and histogram
equalization. The processing techniques have been optimized
in previous work to selectively enhance the retrocardiac and
subdiaphragmatic areas without significant alteration of
detail in the lung. Eight observers were shown 150 test
radiographs (50 unprocessed, 50 processed with adaptive
filtration, 50 processed with histogram equalization)
containing 150 nodules. The results indicate a statistically
significant (P less than .03) difference, with highest
observer performance in the chest radiographs processed with
adaptive filtration (median area under ROC curve = 0.78),
compared with unprocessed images (median = 0.68) and chest
radiographs processed with histogram equalization (median =
0.62). Performance in the lung was not significantly
different. Adaptive filtration applied to selectively
enhance underexposed areas of film images may improve nodule
detection. Histogram equalization provided no improvement in
performance.},
Doi = {10.1148/radiology.166.2.3336719},
Key = {Sherrier88a}
}
@booklet{Stewart00,
Author = {M. D. Stewart and G. A. Johnson and R. C. Burghardt and C.
A. Vyhlidal and S. H. Safe and F. W. Bazer and T. E.
Spencer},
Title = {Effects of interferon tau on short- and long-term activation
of STAT proteins in immortalized ovine uterine luminal
epithelial cells.},
Journal = {Biology Of Reproduction},
Volume = {62},
Pages = {118 -- 118},
Year = {2000},
Key = {Stewart00}
}
@article{fds180938,
Author = {DW Bailey and KA Dunlap and DW Erikson and AK Patel and FW Bazer and RC
Burghardt, GA Johnson},
Title = {Effects of long-term progesterone exposure on porcine
uterine gene expression: progesterone alone does not induce
secreted phosphoprotein 1 (osteopontin) in glandular
epithelium.},
Journal = {Reproduction (Cambridge, England)},
Volume = {140},
Number = {4},
Pages = {595-604},
Year = {2010},
Month = {October},
ISSN = {1741-7899},
url = {http://dx.doi.org/10.1530/REP-10-0169},
Abstract = {Pigs experience significant conceptus loss near
mid-gestation, correlating with increasing glandular
epithelial (GE) development and secretory activity. Secreted
phosphoprotein 1 (SPP1, osteopontin) increases in GE between
days 30 and 40 of pregnancy and is expressed in the GE of
day 90 pseudopregnant pigs, suggesting that progesterone
(P(4)) from corpora lutea is responsible for induction of
SPP1 in GE. In this study, pigs were ovariectomized and
treated daily with P(4) to assess effects of 40 days of P(4)
exposure on SPP1, P(4) receptor (PGR), uteroferrin (ACP5),
and fibroblast growth factor 7 (FGF7) expression in porcine
endometria. PGR mRNA decreased in pigs injected with P(4)
compared with pigs injected with corn oil (CO), and PGRs
were downregulated in the luminal epithelium (LE) and GE.
ACP5 mRNA increased in pigs injected with P(4) compared with
pigs injected with CO, and ACP5 was induced in the GE of
P(4)-treated pigs. FGF7 mRNA increased in pigs injected with
P(4) compared with pigs injected with CO, and FGF7 was
induced in the LE and GE of P(4)-treated pigs. SPP1 mRNA was
not different between pigs injected with P(4) compared with
pigs injected with CO, and SPP1 was not present in the GE of
P(4)-treated pigs. Therefore, long-term P(4), in the absence
of ovarian and/or conceptus factors, does not induce SPP1
expression in GE. We hypothesize that a servomechanism
involving sequential effects of multiple hormones and
cytokines, similar to those for sheep and humans, is
required for GE differentiation and function, including the
synthesis and secretion of SPP1.},
Language = {eng},
Doi = {10.1530/REP-10-0169},
Key = {fds180938}
}
@article{fds180940,
Author = {DW Bailey and KA Dunlap and JW Frank and DW Erikson and BG White and FW
Bazer, RC Burghardt and GA Johnson},
Title = {Effects of long-term progesterone on developmental and
functional aspects of porcine uterine epithelia and
vasculature: progesterone alone does not support development
of uterine glands comparable to that of pregnancy.},
Journal = {Reproduction (Cambridge, England)},
Volume = {140},
Number = {4},
Pages = {583-94},
Year = {2010},
Month = {October},
ISSN = {1741-7899},
url = {http://dx.doi.org/10.1530/REP-10-0170},
Abstract = {In pigs, endometrial functions are regulated primarily by
progesterone and placental factors including estrogen.
Progesterone levels are high throughout pregnancy to
stimulate and maintain secretion of histotroph from uterine
epithelia necessary for growth, implantation, placentation,
and development of the conceptus (embryo and its
extra-embryonic membranes). This study determined effects of
long-term progesterone on development and histoarchitecture
of endometrial luminal epithelium (LE), glandular epithelium
(GE), and vasculature in pigs. Pigs were ovariectomized
during diestrus (day 12), and then received daily injections
of either corn oil or progesterone for 28 days. Prolonged
progesterone treatment resulted in increased weight and
length of the uterine horns, and thickness of the
endometrium and myometrium. Hyperplasia and hypertrophy of
GE were not evident, but LE cell height increased,
suggesting elevated secretory activity. Although GE
development was deficient, progesterone supported increased
endometrial angiogenesis comparable to that of pregnancy.
Progesterone also supported alterations to the apical and
basolateral domains of LE and GE. Dolichos biflorus
agglutinin lectin binding and α(v) integrin were
downregulated at the apical surfaces of LE and GE.
Claudin-4, α(2)β(1) integrin, and vimentin were increased
at basolateral surfaces, whereas occludins-1 and -2,
claudin-3, and E-cadherin were unaffected by progesterone
treatment indicating structurally competent trans-epithelial
adhesion and tight junctional complexes. Collectively, the
results suggest that progesterone affects LE, GE, and
vascular development and histoarchitecture, but in the
absence of ovarian or placental factors, it does not support
development of GE comparable to pregnancy. Furthermore, LE
and vascular development are highly responsive to the
effects of progesterone.},
Language = {eng},
Doi = {10.1530/REP-10-0170},
Key = {fds180940}
}
@booklet{Gibson83,
Author = {J. K. Gibson and J. A. Gifford and P. F. Kane and G. L.
Degraaf and F. G. Robinson and J. P. Hansen and D. W. Harris and G. A. Johnson and H. S. Greenberg and D. W.
Ducharme},
Title = {Effects of non-steroidal anti-inflammatory drugs on
myocardial healing after myocardial-infarction},
Journal = {Federation Proceedings},
Volume = {42},
Number = {3},
Pages = {633 -- 633},
Year = {1983},
Key = {Gibson83}
}
@booklet{Spencer00,
Author = {T. E. Spencer and G. A. Johnson and M. D. Stewart and M. M.
Joyce and C. A. Gray and K. M. Taylor and A. Gertler and E.
Gootwine and F. W. Bazer},
Title = {Effects of ovine placental lactogen and growth hormone on
ovine endometrial function.},
Journal = {Biology Of Reproduction},
Volume = {62},
Pages = {264 -- 264},
Year = {2000},
Key = {Spencer00}
}
@booklet{Lee00,
Author = {C. K. Lee and R. L. Weaks and G. A. Johnson and F. W. Bazer and J. A. Piedrahita},
Title = {Effects of protease inhibitors and antioxidants on in vitro
survival of porcine primordial germ cells},
Journal = {Biology Of Reproduction},
Volume = {63},
Number = {3},
Pages = {887 -- 897},
Year = {2000},
Month = {September},
Key = {Lee00}
}
@article{fds174143,
Author = {CK Lee and RL Weaks and GA Johnson and FW Bazer and JA
Piedrahita},
Title = {Effects of protease inhibitors and antioxidants on In vitro
survival of porcine primordial germ cells.},
Journal = {Biology of reproduction},
Volume = {63},
Number = {3},
Pages = {887-97},
Year = {2000},
Month = {September},
ISSN = {0006-3363},
Keywords = {Acetylcysteine • Animals • Antioxidants •
Apoptosis • Cell Survival • Cells, Cultured •
DNA Fragmentation • Dose-Response Relationship, Drug
• Fetus • Germ Cells • In Situ Nick-End
Labeling • Microscopy, Electron • Protease
Inhibitors • Swine • administration & dosage
• alpha-Macroglobulins • cytology • drug
effects • drug effects* • pharmacology •
pharmacology* • physiology*},
Abstract = {One of the problems associated with in vitro culture of
primordial germ cells (PGCs) is the large loss of cells
during the initial period of culture. This study
characterized the initial loss and determined the
effectiveness of two classes of apoptosis inhibitors,
protease inhibitors, and antioxidants on the ability of
porcine PGCs to survive in culture. Results from electron
microscopic analysis and in situ DNA fragmentation assay
indicated that porcine PGCs rapidly undergo apoptosis when
placed in culture. Additionally, alpha(2)-macroglobulin, a
protease inhibitor and cytokine carrier, and
N:-acetylcysteine, an antioxidant, increased the survival of
PGCs in vitro. While other protease inhibitors tested did
not affect survival of PGCs, all antioxidants tested
improved survival of PGCs (P: < 0.05). Further results
indicated that the beneficial effect of the antioxidants was
critical only during the initial period of culture. Finally,
it was determined that in short-term culture, in the absence
of feeder layers, antioxidants could partially replace the
effect(s) of growth factors and reduce apoptosis.
Collectively, these results indicate that the addition of
alpha(2)-macroglobulin and antioxidants can increase the
number of PGCs in vitro by suppressing apoptosis.},
Language = {eng},
Key = {fds174143}
}
@booklet{Spencer99,
Author = {T. E. Spencer and A. Gray and G. A. Johnson and K. M. Taylor and A. Gertler and E. Gootwine and T. L. Ott and F. W.
Bazer},
Title = {Effects of recombinant ovine interferon tau, placental
lactogen, and growth hormone on the ovine
uterus},
Journal = {Biology Of Reproduction},
Volume = {61},
Number = {6},
Pages = {1409 -- 1418},
Year = {1999},
Month = {December},
Key = {Spencer99}
}
@article{fds174304,
Author = {TE Spencer and A Gray and GA Johnson and KM Taylor and A Gertler and E
Gootwine, TL Ott and FW Bazer},
Title = {Effects of recombinant ovine interferon tau, placental
lactogen, and growth hormone on the ovine
uterus.},
Journal = {Biology of reproduction},
Volume = {61},
Number = {6},
Pages = {1409-18},
Year = {1999},
Month = {December},
ISSN = {0006-3363},
Keywords = {Animals • Corpus Luteum • Endometrium •
Female • Gene Expression • Glycoproteins •
Growth Hormone • Interferon Type I • Ki-67 Antigen
• Ovariectomy • Placental Lactogen •
Pregnancy Proteins • RNA, Messenger • Receptors,
Estrogen • Receptors, Oxytocin • Receptors,
Progesterone • Recombinant Proteins • Serpins*
• Sheep* • analysis • chemistry • drug
effects • genetics • pharmacology •
pharmacology* • physiology • physiology*},
Abstract = {Studies were conducted to determine effects of intrauterine
administration of recombinant ovine interferon tau (IFNtau),
placental lactogen (PL), and growth hormone (GH) on
endometrial function. In the first study, administration of
IFNtau to cyclic ewes for one period (Days 11-15) resulted
in an interestrous interval (IEI) of approximately 30 days,
whereas administration for two periods (Days 11-15 and Days
21-25) extended the IEI to greater than 50 days.
Administration of IFNtau from Days 11 to 15 and of PL or GH
from Days 21 to 25 failed to extend the IEI more than for
IFNtau alone. In the second study, effects of IFNtau, PL,
and GH on endometrial differentiation and function were
determined in ovariectomized ewes receiving ovarian steroid
replacement therapy. Endometrial expression of mRNAs for
estrogen receptor (ER), progesterone receptor (PR), and
oxytocin receptor (OTR) were not affected by PL or GH
treatment; however, uterine milk protein mRNA levels and
stratum spongiosum gland density were increased by both PL
and GH treatments. Collectively, results indicated that 1)
PL and GH do not regulate endometrial PR, ER, and OTR
expression or affect corpus luteum life span; 2)
down-regulation of epithelial PR expression is requisite for
progesterone induction of secretory gene expression in
uterine glandular epithelium; 3) effects of PL and GH on
endometrial function require IFNtau; and 4) PL and GH
regulate endometrial gland proliferation and perhaps
differentiated function.},
Language = {eng},
Key = {fds174304}
}
@booklet{Johnson01b,
Author = {G. A. Johnson and M. D. Stewart and C. A. Gray and Y. Choi and R. C. Burghardt and L. Y. Yu-lee and F. W. Bazer and T.
E. Spencer},
Title = {Effects of the estrous cycle, pregnancy, and interferon tau
on 2 ',5 '-oligoadenylate synthetase expression in the ovine
uterus},
Journal = {Biology Of Reproduction},
Volume = {64},
Number = {5},
Pages = {1392 -- 1399},
Year = {2001},
Month = {May},
Key = {Johnson01b}
}
@article{fds174204,
Author = {GA Johnson, MD Stewart and CA Gray and Y Choi and RC Burghardt and LY
Yu-Lee, FW Bazer and TE Spencer},
Title = {Effects of the estrous cycle, pregnancy, and interferon tau
on 2',5'-oligoadenylate synthetase expression in the ovine
uterus.},
Journal = {Biology of reproduction},
Volume = {64},
Number = {5},
Pages = {1392-9},
Year = {2001},
Month = {May},
ISSN = {0006-3363},
Keywords = {2',5'-Oligoadenylate Synthetase • Animals • Cell
Line • Endometrium • Estrus • Female •
Gene Expression* • Interferon Type I • Pregnancy
• Pregnancy Proteins • Recombinant Proteins •
Sheep • Uterus • administration & dosage •
drug effects • genetics* • metabolism •
metabolism* • pharmacology • pharmacology* •
physiology*},
Abstract = {The enzymes which comprise the 2',5'-oligoadenylate
synthetase (OAS) family are interferon (IFN) stimulated
genes which regulate ribonuclease L antiviral responses and
may play additional roles in control of cellular growth and
differentiation. This study characterized OAS expression in
the endometrium of cyclic and pregnant ewes as well as
determined effects of IFNtau and progesterone on OAS
expression in cyclic or ovariectomized ewes and in
endometrial epithelial and stromal cell lines. In cyclic
ewes, low levels of OAS protein were detected in the
endometrial stroma (S) and glandular epithelium (GE). In
early pregnant ewes, OAS expression increased in the S and
GE on Day 15. OAS expression in the lumenal epithelium (LE)
was not detected in uteri from either cyclic or pregnant
ewes. Intrauterine administration of IFNtau stimulated OAS
expression in the S and GE, and this effect of IFNtau was
dependent on progesterone. Ovine endometrial LE, GE, and S
cell lines responded to IFNtau with induction of OAS
proteins. In all three cell lines, the 40/46-kDa OAS forms
were induced by IFNtau, whereas the 100-kDa OAS form
appeared to be constitutively expressed and not affected by
IFNtau. The 69/71-kDa OAS forms were induced by IFNtau in
the S and GE cell lines, but not in the LE. Collectively,
these results indicate that OAS expression in the
endometrial S and GE of the early pregnant ovine uterus is
directly regulated by IFNtau from conceptus and requires the
presence of progesterone.},
Language = {eng},
Key = {fds174204}
}
@article{fds326004,
Author = {Wolbarsht, ML and O'foghludha, FA and Sliney, DH and Guy, AW and Smith,
AA and Johnson, GA},
Title = {Electromagnetic emission from visual display units: A
non-hazard},
Journal = {Proceedings of SPIE - The International Society for Optical
Engineering},
Volume = {229},
Pages = {187-195},
Publisher = {SPIE},
Year = {1980},
Month = {October},
url = {http://dx.doi.org/10.1117/12.958802},
Abstract = {An analysis of the electromagnetic emissions of an IBM Model
32772 visual display unit showed no hazardous levels in any
portion of the spectrum. The actual level of emission was
measured throughout the spectrum from low frequency radio
waves through x-radiation, extending from 10 KHz through 10
GHz, then 0.2 to 10 μm, and from 5 to over 40 keV. In many
parts of the spectrum, the level of emission was below the
sensitivity of available instrumentation. In the radio
frequency range, including the microwave region,
measurements were also made on black and white and color
television sets for the purpose of comparison. © 1980,
SPIE.},
Doi = {10.1117/12.958802},
Key = {fds326004}
}
@booklet{Johnson99d,
Author = {G. A. Johnson and K. J. Austin and A. M. Collins and W. J.
Murdoch and T. R. Hansen},
Title = {Endometrial ISG17 mRNA and a related mRNA are induced by
interferon-tau and localized to glandular epithelial and
stromal cells from pregnant cows},
Journal = {Endocrine},
Volume = {10},
Number = {3},
Pages = {243 -- 252},
Year = {1999},
Month = {June},
Key = {Johnson99d}
}
@article{fds174237,
Author = {GA Johnson and KJ Austin and AM Collins and WJ Murdoch and TR
Hansen},
Title = {Endometrial ISG17 mRNA and a related mRNA are induced by
interferon-tau and localized to glandular epithelial and
stromal cells from pregnant cows.},
Journal = {Endocrine},
Volume = {10},
Number = {3},
Pages = {243-52},
Year = {1999},
Month = {June},
ISSN = {1355-008X},
url = {http://dx.doi.org/10.1007/BF02738623},
Keywords = {Animals • Blotting, Northern • Cattle • Cell
Line • Endometrium • Epithelial Cells •
Female • In Situ Hybridization • Interferon Type I
• Nuclear Proteins • Pregnancy • Pregnancy
Proteins • RNA, Messenger • Recombinant Proteins
• Stromal Cells • Tissue Distribution •
analysis • biosynthesis* • chemistry •
genetics* • metabolism* • pharmacology •
pharmacology*},
Abstract = {The interferon stimulated gene product, ISG17, conjugates to
bovine uterine proteins in response to conceptus-derived
interferon (IFN)-tau. The objectives of the present
experiments were to examine induction of ISG17 (0.65 kb) and
a related 2.5 kb mRNA in response to IFN-tau and pregnancy
using Northern blotting procedures, and to determine cell
types in the endometrium that expressed ISG17 mRNA using in
situ hybridization. RNA was isolated from endometrial
explants or from bovine endometrial (BEND) cells cultured in
the absence (control) or presence of 25 nM recombinant (r)
bolFN-tau for 0, 3, 6, 12, 24, or 48 h. The major ISG17 0.65
kb mRNA and a minor 2.5 kb mRNA were induced (p<0.05) after
6 h (explants) or 3 h (BEND cells) treatment with
rboIFN-tau. Both mRNAs were present in endometrium from day
18 pregnant cows, but were absent in endometrium from
nonpregnant cows. The ISG17 mRNA was localized to stromal
and glandular epithelial cells on d 18 of pregnancy. The 2.5
kb mRNA may encode a novel ISG17 homolog, or a unique
polyISG17 repeat that is similar in structure to the
polyubiquitin genes. Because ISG17 mRNA is induced in
stromal and glandular epithelial cells, it could be assumed
that ISG17 has a role in regulating intracellular proteins
in both cell types.},
Language = {eng},
Doi = {10.1007/BF02738623},
Key = {fds174237}
}
@booklet{Ede01,
Author = {P. N. Ede and G. A. Johnson},
Title = {Energy relations of gas estimated from flare radiation in
Nigeria},
Journal = {International Journal Of Energy Research},
Volume = {25},
Number = {1},
Pages = {85 -- 91},
Year = {2001},
Month = {January},
Key = {Ede01}
}
@article{fds174233,
Author = {RC Burghardt, JR Burghardt and JD Taylor 2nd and AT Reeder and BT
Nguen, TE Spencer and KJ Bayless and GA Johnson},
Title = {Enhanced focal adhesion assembly reflects increased
mechanosensation and mechanotransduction at
maternal-conceptus interface and uterine wall during ovine
pregnancy.},
Journal = {Reproduction (Cambridge, England)},
Volume = {137},
Number = {3},
Pages = {567-82},
Year = {2009},
Month = {March},
ISSN = {1741-7899},
url = {http://dx.doi.org/10.1530/REP-08-0304},
Keywords = {Animals • Cytoskeletal Proteins • Embryo
Implantation • Extracellular Matrix Proteins •
Female • Fluorescent Antibody Technique • Focal
Adhesions • Integrins • Mechanotransduction,
Cellular • Models, Animal • Pregnancy • Sheep
• Trophoblasts • Uterus • analysis •
metabolism* • physiology • physiology*},
Abstract = {The integrity of the fetal-maternal interface is critical
for proper fetal nourishment during pregnancy. Integrins are
important adhesion molecules present at the interface during
implantation; however, in vivo evidence for integrin
activation and focal adhesion formation at the
maternal-conceptus interface is limited. We hypothesized
that focal adhesion assembly in uterine luminal epithelium
(LE) and conceptus trophectoderm (Tr) results from integrin
binding of extracellular matrix (ECM) at this interface to
provide increased tensile forces and signaling to coordinate
utero-placental development. An ovine model of unilateral
pregnancy was used to evaluate mechanotransduction events
leading to focal adhesion assembly at the maternal-conceptus
interface and within the uterine wall. Animals were
hysterectomized on days 40, 80, or 120 of pregnancy, and
uteri immunostained for integrins (ITGAV, ITGA4, ITGA5,
ITGB1, ITGB3, and ITGB5), ECM proteins (SPP1, LGALS15,
fibronectin (FN), and vitronectin (VTN)), cytoskeletal
molecules (ACTN and TLN1), and a signal generator (PTK2).
Focal adhesion assembly in myometrium and stroma was also
studied to provide a frame of reference for mechanical
stretch of the uterine wall. Large focal adhesions
containing aggregates of ITGAV, ITGA4, ITGA5, ITGB1, ITGB5,
ACTN, and PTK2 were detected in interplacentomal uterine LE
and Tr of gravid but not non-gravid uterine horns and
increased during pregnancy. SPP1 and LGALS15, but not FN or
VTN, were present along LE and Tr interfaces in both uterine
horns. These data support the idea that focal adhesion
assembly at the maternal-conceptus interface reflects
adaptation to increasing forces caused by the growing fetus.
Cooperative binding of multiple integrins to SPP1 deposited
at the maternal-conceptus interface forms an adhesive mosaic
to maintain a tight connection between uterine and placental
surfaces along regions of epitheliochorial placentation in
sheep.},
Language = {eng},
Doi = {10.1530/REP-08-0304},
Key = {fds174233}
}
@article{fds174128,
Author = {DJ Waldon and TT Kawabe and CA Baker and GA Johnson and AE
Buhl},
Title = {Enhanced in vitro hair growth at the air-liquid interface:
minoxidil preserves the root sheath in cultured whisker
follicles.},
Journal = {In vitro cellular & developmental biology.
Animal},
Volume = {29A},
Number = {7},
Pages = {555-61},
Year = {1993},
Month = {July},
ISSN = {1071-2690},
Keywords = {Air • Animals • Cells, Cultured •
Collagenases • Cysteine • Gelatin Sponge,
Absorbable • Glycoproteins • Guanidines •
Hair • Keratins • Mice • Minoxidil •
Pyridines • Sulfotransferases • Tissue Inhibitor
of Metalloproteinases • Vibrissae • analysis
• antagonists & inhibitors • chemistry •
cytology* • growth & development* • metabolism
• pharmacology • pharmacology*},
Abstract = {Inasmuch as hair follicles are difficult to maintain in
culture, the study of hair biology using cultured hair
follicles has met with only limited success. In our attempts
to solve the problem of follicle degeneration, we cultured
follicles at the air-surface interface on a modified
collagen matrix (Gelfoam). In follicles cultured at the
air-surface or submerged, we examined follicular morphology,
hair shaft growth, sulfotransferase levels, cysteine
incorporation, an expression of a tissue inhibitor of
metalloproteinase (TIMP), and ultra-high sulfur keratin
(UHSK). Follicles cultured at the air-liquid interface
produced a 2.7-fold increase in hair growth and maintained
an anagen-like morphology. Substrates such as nylon mesh
seeded with fibroblasts, Full Thickness Skin, or 5-microns
polycarbonate filter also supported hair growth, whereas
Gelfilm, GF-A glass filter, filter paper, or 1-micron
polycarbonate filter did not. The UHSK expression was
significantly higher in the air-liquid interface cultures
compared to the submerged culture. Several potassium channel
openers, including minoxidil, a minoxidil analog, and the
pinacidil analog (P-1075), all stimulated significant
cysteine incorporation in follicles. Minoxidil and its
analog specifically preserved the follicular root sheath, in
contrast to P-1075 which did not, indicating a difference in
the two drug types. The preservation of the root sheath was
measured by increased TIMP expression and sulfotransferase
activity and indicates that the root sheath is a target
tissue for minoxidil. Our results show that follicles
cultured at the air-liquid interface maintain a better
morphology and produced greater hair growth than follicles
cultured on tissue culture plastic.},
Language = {eng},
Key = {fds174128}
}
@booklet{Waldon93,
Author = {D. J. Waldon and T. T. Kawabe and C. A. Baker and G. A.
Johnson and A. E. Buhl},
Title = {Enhanced in-vitro hair-growth at the air-liquid interface -
minoxidil preserves the root sheath in cultured whisker
follicles},
Journal = {In Vitro Cellular \& Developmental Biology-animal},
Volume = {29A},
Number = {7},
Pages = {555 -- 561},
Year = {1993},
Month = {July},
Key = {Waldon93}
}
@article{fds157113,
Author = {AA Sharief and GA Johnson},
Title = {Enhanced T2 contrast for MR histology of the mouse
brain.},
Journal = {Magnetic resonance in medicine : official journal of the
Society of Magnetic Resonance in Medicine / Society of
Magnetic Resonance in Medicine, United States},
Volume = {56},
Number = {4},
Pages = {717-25},
Year = {2006},
Month = {October},
ISSN = {0740-3194},
Keywords = {Animals • Brain Mapping • Contrast Media •
Heterocyclic Compounds • Imaging, Three-Dimensional
• Least-Squares Analysis • Magnetic Resonance
Imaging • Male • Mice • Mice, Inbred C57BL
• Organometallic Compounds • Staining and Labeling
• methods*},
Abstract = {A 3D Carr-Purcell-Meiboom-Gill (CPMG) sequence was
implemented to obtain enhanced T(2) contrast in actively
stained (perfusion with fixative and contrast agent) mouse
brains at 9.4 T. Short interecho spacing was used to
minimize diffusion and susceptibility losses. The sequence
produced 16 3D volumes with an interecho spacing of 7 ms for
isotropic 43-mu-resolution images of the mouse brains in a
scan time of 4 hr. To enhance the signal-to-noise ratio
(SNR) and contrast, the multiecho frequency domain image
contrast (MEFIC) method was applied, resulting in a
composite image with T(2)-weighted contrast. The high SNR
and contrast thus achieved revealed aspects of mouse brain
morphology, such as multiple cortical layers, groups of
thalamic nuclei, layers of the inferior and superior
colliculus, and molecular and granular layers of the
cerebellum, with a high degree of definition and contrast
that was not previously achieved in T(2)-weighted
acquisitions at high fields.},
Key = {fds157113}
}
@article{9150726,
Author = {Sharief, AA and Johnson, GA},
Title = {Enhanced T2 contrast for MR histology of the mouse
brain.},
Journal = {Magn Reson Med},
Volume = {56},
Number = {4},
Pages = {717-725},
Year = {2006},
Month = {October},
ISSN = {0740-3194},
url = {http://www.ncbi.nlm.nih.gov/pubmed/16964618},
Keywords = {biomedical MRI;brain;data acquisition;image
sequences;neurophysiology;},
Abstract = {A 3D Carr-Purcell-Meiboom-Gill (CPMG) sequence was
implemented to obtain enhanced T(2) contrast in actively
stained (perfusion with fixative and contrast agent) mouse
brains at 9.4 T. Short interecho spacing was used to
minimize diffusion and susceptibility losses. The sequence
produced 16 3D volumes with an interecho spacing of 7 ms for
isotropic 43-mu-resolution images of the mouse brains in a
scan time of 4 hr. To enhance the signal-to-noise ratio
(SNR) and contrast, the multiecho frequency domain image
contrast (MEFIC) method was applied, resulting in a
composite image with T(2)-weighted contrast. The high SNR
and contrast thus achieved revealed aspects of mouse brain
morphology, such as multiple cortical layers, groups of
thalamic nuclei, layers of the inferior and superior
colliculus, and molecular and granular layers of the
cerebellum, with a high degree of definition and contrast
that was not previously achieved in T(2)-weighted
acquisitions at high fields.},
Doi = {10.1002/mrm.21026},
Key = {9150726}
}
@booklet{Johnson02,
Author = {G. A. Johnson and T. A. Day},
Title = {Enhancement of photosynthesis in Sorghum bicolor by
ultraviolet radiation},
Journal = {Physiologia Plantarum},
Volume = {116},
Number = {4},
Pages = {554 -- 562},
Year = {2002},
Month = {December},
Key = {Johnson02}
}
@article{fds132898,
Author = {KD Meisheri and GA Johnson and L Puddington},
Title = {Enzymatic and non-enzymatic sulfation mechanisms in the
biological actions of minoxidil.},
Journal = {Biochemical pharmacology, ENGLAND},
Volume = {45},
Number = {2},
Pages = {271-9},
Year = {1993},
Month = {January},
ISSN = {0006-2952},
Keywords = {Animals • Biotransformation • Humans •
Minoxidil • Muscle, Smooth, Vascular • Potassium
Channels • Proteins • Sulfotransferases •
Triazines • Vasodilation • drug effects •
metabolism • metabolism* • pharmacokinetics •
pharmacology},
Key = {fds132898}
}
@booklet{Meisheri93,
Author = {K. D. Meisheri and G. A. Johnson and L. Puddington},
Title = {Enzymatic and nonenzymatic sulfation mechanisms in the
biological actions of minoxidil},
Journal = {Biochemical Pharmacology},
Volume = {45},
Number = {2},
Pages = {271 -- 279},
Year = {1993},
Month = {January},
Key = {Meisheri93}
}
@booklet{Johnson97c,
Author = {G. A. Johnson and R. Mannel and M. Khalifa and J. L. Walker and M. Wren and K. W. Min and D. M. Benbrook},
Title = {Epidermal growth factor receptor in vulvar malignancies and
its relationship to metastasis and patient
survival},
Journal = {Gynecologic Oncology},
Volume = {65},
Number = {3},
Pages = {425 -- 429},
Year = {1997},
Month = {June},
Key = {Johnson97c}
}
@article{fds174146,
Author = {GA Johnson and R Mannel and M Khalifa and JL Walker and M Wren and KW Min and DM Benbrook},
Title = {Epidermal growth factor receptor in vulvar malignancies and
its relationship to metastasis and patient
survival.},
Journal = {Gynecologic oncology},
Volume = {65},
Number = {3},
Pages = {425-9},
Year = {1997},
Month = {June},
ISSN = {0090-8258},
url = {http://dx.doi.org/10.1006/gyno.1997.4660},
Keywords = {Carcinoma, Squamous Cell • Disease-Free Survival •
Female • Humans • Lymphatic Metastasis •
Receptor, Epidermal Growth Factor • Survival Rate
• Vulvar Neoplasms • biosynthesis* •
metabolism* • mortality • mortality* •
pathology},
Abstract = {OBJECTIVE: To evaluate the level of epidermal growth factor
receptor (EGF-R) expression in vulvar malignancies and to
determine if a correlation exists between EGF-R levels and
metastasis or patient survival. METHODS: All patients with a
diagnosis of invasive squamous cell carcinoma of the vulva
who were treated at our institution with a primary radical
vulvectomy and inguinal lymph node dissection from 1983 to
1993 were eligible for the study. Sixty-one patients with
available tissue blocks of benign vulvar epithelium, the
primary malignant vulvar lesion, and groin node metastasis
(when positive) were included in the study. Semiquantitative
EGF-R expression was determined in a blinded fashion
utilizing immunohistochemical staining of appropriate tissue
samples. Survival was calculated utilizing Kaplan-Meier life
table analysis based upon disease-free survival. RESULTS: A
significant increase (P < 0.001) in mean EGF-R levels was
demonstrated in the primary tumor (67%) versus benign vulvar
epithelium (31%). In the 14 patients with lymph node
metastasis, the mean EGF-R level in the primary tumor was
65% versus 88% in the metastatic lesion (P < 0.001). The
likelihood of lymph node metastasis was elevated in those
patients with a benign tissue EGF-R level > or =40% (P <
0.03) and in those patients with a primary tumor EGF-R level
> or =90% (P < 0.025). Life table analysis revealed a
cumulative disease-free survival of 45% for all patients.
Disease-free survival in those patients with EGF-R levels >
or =90% in the primary tumor was 25%, contrasting with a
disease-free survival of 54% in those patients with EGF-R
levels <90% (P < 0.05). CONCLUSIONS: There is a progressive
increase in EGF-R expression from benign vulvar epithelium
to primary malignant tissue to metastatic lesions within the
same patient. Increased expression of EGF-R in the primary
vulvar malignancy is significantly associated with lymph
node metastasis and decreased patient survival. Increased
expression of EGF-R in histologically benign vulvar
epithelium has a significant association with lymph node
metastasis and may predict decreased patient
survival.},
Language = {eng},
Doi = {10.1006/gyno.1997.4660},
Key = {fds174146}
}
@booklet{Choi01a,
Author = {Y. Choi and G. A. Johnson and R. C. Burghardt and L. R.
Berghman and M. M. Joyce and T. E. Spencer and F. W.
Bazer},
Title = {Epithelial expression of interferon regulatory factor two
restricts expression of interferon tau-stimulated genes to
the endometrial stroma and glandular epithelium of the ovine
uterus.},
Journal = {Biology Of Reproduction},
Volume = {64},
Pages = {108 -- 108},
Year = {2001},
Key = {Choi01a}
}
@article{fds268763,
Author = {Smith, BR and Sattuck, MD and Hedlund, LW and Johnson,
GA},
Title = {Erratum: Time-course imaging of rat embryos in utero with
magnetic resonance microscopy (Magnetic Resonance in
Medicine (1998) 39 (673-677))},
Journal = {Magnetic Resonance in Medicine},
Volume = {39},
Number = {6},
Pages = {x},
Year = {1998},
Month = {January},
Key = {fds268763}
}
@article{fds174255,
Author = {BM Markaverich and RR Gregory and MA Alejandro and RS Varma and GA
Johnson, BS Middleditch},
Title = {Estrogen regulation of methyl p-hydroxyphenyllactate
hydrolysis: correlation with estrogen stimulation of rat
uterine growth.},
Journal = {Journal of steroid biochemistry},
Volume = {33},
Number = {5},
Pages = {867-76},
Year = {1989},
Month = {November},
ISSN = {0022-4731},
Keywords = {Animals • Cell Compartmentation • Chromatography
• Cytosol • Esterases • Estrogens •
Female • Hot Temperature • Kinetics •
Lactates • Phenylpropionates • Rats •
Substrate Specificity • Uterus • enzymology*
• metabolism • metabolism* •
physiology*},
Abstract = {We have recently demonstrated that methyl
p-hydroxyphenyllactate (MeHPLA) is the endogenous ligand for
nuclear type II binding sites in the rat uterus and other
estrogen target and non-target tissues. MeHPLA binds to
nuclear type II binding sites with a very high binding
affinity (Kd approximately 4-5 nM), blocks uterine growth in
vivo, and inhibits MCF-7 human breast cancer cell growth in
vitro. Conversely, the free acid (p-hydroxyphenyllactic
acid, HPLA) interacts with type II binding sites with a much
lower affinity (Kd approximately 200 nM) and does not
inhibit estrogen-induced uterine growth in vivo or MCF-7
cell growth in vitro. On the basis of these observations, we
suggested that one way that estrogen may override MeHPLA
inhibition of rat uterine growth may be to stimulate
esterase hydrolysis of MeHPLA to HPLA. The present studies
demonstrate that the rat uterus does contain an esterase
(mol. wt approximately 50,000) which cleaves MeHPLA to HPLA,
and that this enzyme is under estrogen regulation. This
conclusion is supported by the observations that MeHPLA
esterase activity is increased 2-3-fold above controls
within 2-4 h following a single injection of estradiol, and
is maintained at high levels for 16-24 h following hormone
administration. This sustained elevation of MeHPLA esterase
activity correlates with estradiol stimulation of true
uterine growth and DNA synthesis.},
Language = {eng},
Key = {fds174255}
}
@booklet{Markaverich89,
Author = {B. M. Markaverich and R. R. Gregory and M. A. Alejandro and R. S. Varma and G. A. Johnson and B. S. Middleditch},
Title = {Estrogen regulation of methyl para-hydroxyphenylactate
hydrolysis - correlation with estrogen stimulation of rat
uterine growth},
Journal = {Journal Of Steroid Biochemistry And Molecular
Biology},
Volume = {33},
Number = {5},
Pages = {867 -- 876},
Year = {1989},
Month = {November},
Key = {Markaverich89}
}
@article{fds268774,
Author = {Lipinski, RJ and Hammond, P and O'Leary-Moore, SK and Ament, JJ and Pecevich, SJ and Jiang, Y and Budin, F and Parnell, SE and Suttie, M and Godin, EA and Everson, JL and Dehart, DB and Oguz, I and Holloway, HT and Styner, MA and Johnson, GA and Sulik, KK},
Title = {Ethanol-induced face-brain dysmorphology patterns are
correlative and exposure-stage dependent.},
Journal = {PLoS One},
Volume = {7},
Number = {8},
Pages = {e43067},
Year = {2012},
ISSN = {1932-6203},
url = {http://dx.doi.org/10.1371/journal.pone.0043067},
Abstract = {Prenatal ethanol exposure is the leading preventable cause
of congenital mental disability. Whereas a diagnosis of
fetal alcohol syndrome (FAS) requires identification of a
specific pattern of craniofacial dysmorphology, most
individuals with behavioral and neurological sequelae of
heavy prenatal ethanol exposure do not exhibit these
defining facial characteristics. Here, a novel integration
of MRI and dense surface modeling-based shape analysis was
applied to characterize concurrent face-brain phenotypes in
C57Bl/6J fetuses exposed to ethanol on gestational day (GD)7
or GD8.5. The facial phenotype resulting from ethanol
exposure depended upon stage of insult and was predictive of
unique patterns of corresponding brain abnormalities.
Ethanol exposure on GD7 produced a constellation of
dysmorphic facial features characteristic of human FAS,
including severe midfacial hypoplasia, shortening of the
palpebral fissures, an elongated upper lip, and deficient
philtrum. In contrast, ethanol exposure on GD8.5 caused mild
midfacial hypoplasia and palpebral fissure shortening, a
shortened upper lip, and a preserved philtrum. These
distinct, stage-specific facial phenotypes were associated
with unique volumetric and shape abnormalities of the septal
region, pituitary, and olfactory bulbs. By demonstrating
that early prenatal ethanol exposure can cause more than one
temporally-specific pattern of defects, these findings
illustrate the need for an expansion of current diagnostic
criteria to better capture the full range of facial and
brain dysmorphology in fetal alcohol spectrum
disorders.},
Doi = {10.1371/journal.pone.0043067},
Key = {fds268774}
}
@booklet{Sherrier85b,
Author = {SHERRIER, RH and CHILES, C and SUDDARTH, SA and JOHNSON, GA and RAVIN,
CE},
Title = {EVALUATION OF COMPUTER SYNTHESIZED PULMONARY
NODULES},
Journal = {INVESTIGATIVE RADIOLOGY},
Volume = {20},
Number = {6},
Pages = {S27-S27},
Publisher = {LIPPINCOTT-RAVEN PUBL},
Year = {1985},
Month = {January},
ISSN = {0020-9996},
url = {http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:A1985ARG0500124&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=47d3190e77e5a3a53558812f597b0b92},
Key = {Sherrier85b}
}
@booklet{Johnson81a,
Author = {Johnson, GA and Korobkin, M and Heinz, ER},
Title = {Evaluation of multiplanar imaging capabilities of four
current computed tomography (CT) scanners},
Journal = {Proceedings of SPIE - The International Society for Optical
Engineering},
Volume = {273},
Pages = {318-325},
Publisher = {SPIE},
Year = {1981},
Month = {July},
url = {http://dx.doi.org/10.1117/12.931819},
Abstract = {Four state of the art CT scanners were evaluated with
respect to optimal techniques for multiplanar imaging. The
four scanners were a G. E. 8800, Pfizer 0^50, Picker
Synerview 600 and Siemens Somatom 2. Patient movement
artefacts can be minimized by choice of techniques that
provide rapid data acquisition. By deferring reconstruction,
using batch mode acquisition, suppressing screen display and
operator interaction and minimizing tube loading as many as
33 thin slices can be acquired in < 8 minutes. This rapid
scan technique makes use of the narrow collimation over a
large (5-0 cm) distance quite reasonable. A high contrast
star resolution phantom was scanned using these rapid scan
techniques. The multiplanar Images produced from narrow
slices are much higher resolution than those created with
more widely collimated slices. A low contrast (2.5%)
resolution object scanned with the same rapid acquisition
method shows improved resolution for the narrow collimation
even in the presence of increased noise accompanying the
narrow collimation. © 1981 SPIE.},
Doi = {10.1117/12.931819},
Key = {Johnson81a}
}
@article{fds268818,
Author = {Ghaghada, KB and Badea, CT and Karumbaiah, L and Fettig, N and Bellamkonda, RV and Johnson, GA and Annapragada,
A},
Title = {Evaluation of tumor microenvironment in an animal model
using a nanoparticle contrast agent in computed tomography
imaging.},
Journal = {Acad Radiol},
Volume = {18},
Number = {1},
Pages = {20-30},
Year = {2011},
Month = {January},
ISSN = {1878-4046},
url = {http://www.ncbi.nlm.nih.gov/pubmed/21145026},
Keywords = {Animals • Contrast Media • Disease Models, Animal
• Female • Imaging, Three-Dimensional •
Mammary Neoplasms, Experimental • Mice • Mice,
Inbred BALB C • Nanoparticles • Radiographic Image
Enhancement • Reverse Transcriptase Polymerase Chain
Reaction • Tomography, X-Ray Computed •
Triiodobenzoic Acids • Tumor Microenvironment* •
blood supply* • diagnostic use • diagnostic use*
• methods • methods* • radiography*},
Abstract = {RATIONALE AND OBJECTIVES: Non-invasive longitudinal imaging
of tumor vasculature could provide new insights into the
development of solid tumors, facilitating efficient delivery
of therapeutics. In this study, we report three-dimensional
imaging and characterization of tumor vascular architecture
using a nanoparticle contrast agent and high-resolution
computed tomography (CT) imaging. MATERIALS AND METHODS:
Five Balb/c mice implanted with 4T1/Luc syngeneic breast
tumors cells were used for the study. The nanoparticle
contrast agent was systemically administered and
longitudinal CT imaging was performed pre-contrast and at
serial time points post-contrast, for up to 7 days for
studying the characteristics of tumor-associated blood
vessels. Gene expression of tumor angiogenic biomarkers was
measured using quantitative real-time polymerase chain
reaction. RESULTS: Early-phase imaging demonstrated the
presence of co-opted and newly developed tumor vessels. The
co-opted vessels demonstrated wall-permeability and
"leakiness" characteristics evident by an increase in
extravascular nanoparticle-based signal enhancement visible
well beyond the margins of tumor. Diameters of
tumor-associated vessels were larger than the contralateral
normal vessels. Delayed-phase imaging also demonstrated
significant accumulation of nanoparticle contrast agent both
within and in areas surrounding the tumor. A heterogeneous
pattern of signal enhancement was observed both within and
among individual tumors. Gene-expression profiling
demonstrated significant variability in several angiogenic
biomarkers both within and among individual tumors.
CONCLUSIONS: The nanoparticle contrast agent and
high-resolution CT imaging facilitated visualization of
co-opted and newly developed tumors vessels as well as
imaging of nanoparticle accumulation within tumors. The use
of this agent could provide novel insights into tumor
vascular biology and could have implications on the
monitoring of tumor status.},
Language = {eng},
Doi = {10.1016/j.acra.2010.09.003},
Key = {fds268818}
}
@booklet{Muggia04,
Author = {F. M. Muggia and J. A. Blessing and M. Method and D. S.
Miller and G. A. Johnson and R. B. Lee and A.
Menzin},
Title = {Evaluation of vinorelbine in persistent or recurrent
squamous cell carcinoma of the cervix: a Gynecologic
Oncology Group study},
Journal = {Gynecologic Oncology},
Volume = {92},
Number = {2},
Pages = {639 -- 643},
Year = {2004},
Month = {February},
Key = {Muggia04}
}
@article{fds174223,
Author = {FM Muggia and JA Blessing and M Method and DS Miller and GA Johnson and RB
Lee, A Menzin and Gynecologic Oncology Group
study},
Title = {Evaluation of vinorelbine in persistent or recurrent
squamous cell carcinoma of the cervix: a Gynecologic
Oncology Group study.},
Journal = {Gynecologic oncology},
Volume = {92},
Number = {2},
Pages = {639-43},
Year = {2004},
Month = {February},
ISSN = {0090-8258},
url = {http://dx.doi.org/10.1016/j.ygyno.2003.10.045},
Keywords = {Adult • Aged • Antineoplastic Agents, Phytogenic
• Carcinoma, Squamous Cell • Drug Administration
Schedule • Female • Humans • Middle Aged
• Uterine Cervical Neoplasms • Vinblastine •
analogs & derivatives* • drug therapy* •
therapeutic use*},
Abstract = {PURPOSE: Vinorelbine is being explored by the Gynecologic
Oncology Group (GOG) for its possible use in advanced or
recurrent squamous cell carcinoma of the uterine cervix. The
objective of this Phase II trial was to evaluate a days 1
and 8 every-21-days schedule and determine its activity in
patients who had failed standard chemotherapy. PATIENTS AND
METHODS: Eligible patients with measurable disease and
satisfactory baseline bone marrow, liver, and kidney
functions were treated with vinorelbine 30 mg/m(2) given on
days 1 and 8 every 21 days. A two-stage sampling design was
used, proceeding to a second stage accrual if sufficient
activity was documented in the first 25 patients. RESULTS:
The study did proceed to the second stage and accrued 44
patients. There were six objective responses (one complete,
five partial) for a response rate of 13.7% (95% confidence
interval: 5.2-27.4%). There were three patients with
response in extra-pelvic sites (including the complete
response) and three with response in the pelvis. The overall
frequency of grades 3 and 4 neutropenia was 41%, whereas
neuropathy was reported in 27% and was severe in three.
Treatment-related pain, very severe in two instances, was
also reported in 27%. CONCLUSION: Vinorelbine has moderate
activity in a pretreated population with squamous cell
carcinoma of the cervix. Accordingly, vinorelbine in this
days 1 and 8 schedule is being studied further in
combination with cisplatin by the GOG.},
Language = {eng},
Doi = {10.1016/j.ygyno.2003.10.045},
Key = {fds174223}
}
@booklet{Joyce01,
Author = {M. M. Joyce and T. E. Spencer and F. W. Bazer and R. C.
Burghardt and C. Pfarrer and G. A. Johnson},
Title = {Evidence for stromal decidualization in the pregnant ovine
uterus.},
Journal = {Biology Of Reproduction},
Volume = {64},
Pages = {317 -- 318},
Year = {2001},
Key = {Joyce01}
}
@booklet{Mantha01,
Author = {S. V. Mantha and G. A. Johnson and T. A.
Day},
Title = {Evidence from action and fluorescence spectra that
UV-induced violet-blue-green fluorescence enhances leaf
photosynthesis},
Journal = {Photochemistry And Photobiology},
Volume = {73},
Number = {3},
Pages = {249 -- 256},
Year = {2001},
Month = {March},
Key = {Mantha01}
}
@article{fds174166,
Author = {SV Mantha and GA Johnson and TA Day},
Title = {Evidence from action and fluorescence spectra that
UV-induced violet-blue-green fluorescence enhances leaf
photosynthesis.},
Journal = {Photochemistry and photobiology},
Volume = {73},
Number = {3},
Pages = {249-56},
Year = {2001},
Month = {March},
ISSN = {0031-8655},
Keywords = {Angiosperms • Photosynthesis • Plant Leaves •
Spectrometry, Fluorescence • Ultraviolet Rays* •
physiology • radiation effects*},
Abstract = {We assessed the contribution of UV-induced violet-blue-green
leaf fluorescence to photosynthesis in Poa annua, Sorghum
halepense and Nerium oleander by measuring UV-induced
fluorescence spectra (280-380 nm excitation, 400-550 nm
emission) from leaf surfaces and determining the
monochromatic UV action spectra for leaf photosynthetic
O2-evolution. Peak fluorescence emission wavelengths from
leaf surfaces ranged from violet (408 nm) to blue (448 nm),
while excitation peaks for these maxima ranged from 333 to
344 nm. Action spectra were developed by supplementing
monochromatic radiation from 280 to 440 nm, in 20 nm
increments, to a visible nonsaturating background of 500
mumol m-2 s-1 photosynthetically active radiation and
measuring photosynthetic O2-evolution rates. Photosynthetic
rates tended to be higher with the 340 nm supplement than
with higher or lower wavelength UV supplements. Comparing
photosynthetic rates with the 340 nm supplement to those
with the 400 nm supplement, the percentage enhancement in
photosynthetic rates at 340 nm ranged from 7.8 to 9.8%. We
suspect that 340 nm UV improves photosynthetic rates via
fluorescence that provides violet-blue-green photons for
photosynthetic energy conversion because (1) the peak
excitation wavelength (340 nm) for violet-blue-green
fluorescence from leaves was also the most effective UV
wavelength at enhancing photosynthetic rates, and (2) the
magnitude of photosynthetic enhancements attributable to
supplemental 340 nm UV was well correlated (R2 = 0.90) with
the apparent intensity of 340 nm UV-induced
violet-blue-green fluorescence emission from
leaves.},
Language = {eng},
Key = {fds174166}
}
@booklet{Gray02,
Author = {C. A. Gray and R. C. Burghardt and G. A. Johnson and F. W.
Bazer and T. E. Spencer},
Title = {Evidence that absence of endometrial gland secretions in
uterine gland knockout ewes compromises conceptus survival
and elongation},
Journal = {Reproduction},
Volume = {124},
Number = {2},
Pages = {289 -- 300},
Year = {2002},
Month = {August},
Key = {Gray02}
}
@article{fds174138,
Author = {CA Gray and RC Burghardt and GA Johnson and FW Bazer and TE
Spencer},
Title = {Evidence that absence of endometrial gland secretions in
uterine gland knockout ewes compromises conceptus survival
and elongation.},
Journal = {Reproduction (Cambridge, England)},
Volume = {124},
Number = {2},
Pages = {289-300},
Year = {2002},
Month = {August},
ISSN = {1470-1626},
Keywords = {Animals • Blastocyst • Blotting, Western •
Embryonic and Fetal Development • Endometrium •
Female • Integrins • Interferon Type I •
Mucin-1 • Mucins • Osteopontin • Pregnancy
• Pregnancy Proteins • Pregnancy, Animal •
Progesterone • Sheep • Sialoglycoproteins •
Uterus • analysis • anatomy & histology •
blood • chemistry • metabolism* • physiology*
• secretion*},
Abstract = {Endometrial glands are necessary for conceptus implantation
and growth. In the ovine uterine gland knockout (UGKO)
model, blastocysts hatch normally but fail to survive or
elongate. This peri-implantation defect in UGKO ewes may be
due to the absence of endometrial glands or, alternatively,
to the lack of certain epithelial adhesion molecules or the
inability of the endometrium to respond to signals from the
conceptus. Two studies were performed to examine these
hypotheses. In study one, normal (n = 8) and UGKO (n = 12)
ewes were mated at oestrus (day 0) with intact rams and
their uteri were flushed 14 days after oestrus. Normal ewes
(n = 4) were also flushed on 14 days after oestrus. Uterine
flushes from bred normal ewes contained filamentous
conceptuses (n = 7 of 8), whereas those from UGKO ewes
contained no conceptus (n = 5 of 12), a growth-retarded,
tubular conceptus (n = 6 of 12), or a fragmented,
filamentous conceptus (n = 1 of 12). In all groups,
expression of mucin 1 and integrin alpha(v), alpha(5),
beta(3) and beta(5) was localized at the apical surface of
the endometrial luminal epithelium with no detectable
differences between normal and UGKO ewes. Uterine flushes
from pregnant ewes, but not cyclic or UGKO ewes, contained
abundant immunoreactive interferon tau and the cell adhesion
proteins, osteopontin and glycosylation-dependent cell
adhesion molecule one. In study two, UGKO ewes were fitted
with uterine catheters 5 days after oestrus, infused with
recombinant ovine interferon tau or control proteins from 11
to 15 days after oestrus, and underwent hysterectomy 16 days
after oestrus. Expression of several interferon
tau-stimulated genes (ISG17, STAT1, STAT2 and IRF-1) was
increased in the endometrium from interferon tau-infused
UGKO ewes. These results support the hypothesis that the
defects in conceptus elongation and survival in UGKO ewes
are due to the absence of endometrial glands and their
secretions rather than to alterations in expression of
anti-adhesive or adhesive molecules on the endometrial
luminal epithelium or to the responsiveness of the
endometrium to the conceptus pregnancy recognition
signal.},
Language = {eng},
Key = {fds174138}
}
@article{fds357199,
Author = {Blocker, SJ and Cook, J and Mowery, YM and Everitt, JI and Qi, Y and Hornburg, KJ and Cofer, GP and Zapata, F and Bassil, AM and Badea, CT and Kirsch, DG and Johnson, GA},
Title = {Ex Vivo MR Histology and Cytometric Feature Mapping Connect
Three-dimensional in Vivo MR Images to Two-dimensional
Histopathologic Images of Murine Sarcomas.},
Journal = {Radiol Imaging Cancer},
Volume = {3},
Number = {3},
Pages = {e200103},
Year = {2021},
Month = {May},
url = {http://dx.doi.org/10.1148/rycan.2021200103},
Abstract = {Purpose To establish a platform for quantitative
tissue-based interpretation of cytoarchitecture features
from tumor MRI measurements. Materials and Methods In a
pilot preclinical study, multicontrast in vivo MRI of murine
soft-tissue sarcomas in 10 mice, followed by ex vivo MRI of
fixed tissues (termed MR histology), was performed.
Paraffin-embedded limb cross-sections were stained with
hematoxylin-eosin, digitized, and registered with MRI.
Registration was assessed by using binarized tumor maps and
Dice similarity coefficients (DSCs). Quantitative cytometric
feature maps from histologic slides were derived by using
nuclear segmentation and compared with registered MRI,
including apparent diffusion coefficients and transverse
relaxation times as affected by magnetic field heterogeneity
(T2* maps). Cytometric features were compared with each MR
image individually by using simple linear regression
analysis to identify the features of interest, and the
goodness of fit was assessed on the basis of R2 values.
Results Registration of MR images to histopathologic slide
images resulted in mean DSCs of 0.912 for ex vivo MR
histology and 0.881 for in vivo MRI. Triplicate repeats
showed high registration repeatability (mean DSC, >0.9).
Whole-slide nuclear segmentations were automated to detect
nuclei on histopathologic slides (DSC = 0.8), and feature
maps were generated for correlative analysis with MR images.
Notable trends were observed between cell density and in
vivo apparent diffusion coefficients (best line fit: R2 =
0.96, P < .001). Multiple cytoarchitectural features
exhibited linear relationships with in vivo T2* maps,
including nuclear circularity (best line fit: R2 = 0.99, P <
.001) and variance in nuclear circularity (best line fit: R2
= 0.98, P < .001). Conclusion An infrastructure for
registering and quantitatively comparing in vivo tumor MRI
with traditional histologic analysis was successfully
implemented in a preclinical pilot study of soft-tissue
sarcomas. Keywords: MRI, Pathology, Animal Studies, Tissue
Characterization Supplemental material is available for this
article. © RSNA, 2021.},
Doi = {10.1148/rycan.2021200103},
Key = {fds357199}
}
@article{fds268744,
Author = {BENVENISTE, H and JOHNSON, GA and KOZNIEWSKA, E and MAIESE, K and NARITOMI, H and POVLISHOCK, JT and HOSSMANN, KA},
Title = {EXCITOTOXICITY AND ISCHEMIA IN THE RAT-BRAIN STUDIED IN-VIVO
WITH HIGH-RESOLUTION MAGNETIC-RESONANCE-IMAGING},
Journal = {MICROCIRCULATORY STASIS IN THE BRAIN},
Volume = {1031},
Pages = {369-376},
Publisher = {ELSEVIER SCIENCE PUBL B V},
Editor = {Tomita, M and Mchedlishvili, F and Rosenblum, W and Heiss, WD and Fukuuchi, Y},
Year = {1993},
Month = {January},
ISBN = {0-444-89669-4},
ISSN = {0531-5131},
url = {http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:A1993BZ68Q00043&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=47d3190e77e5a3a53558812f597b0b92},
Key = {fds268744}
}
@article{fds174149,
Author = {GA Johnson and TK Hung and AM Brant and HS Borovetz},
Title = {Experimental determination of wall shear rate in canine
carotid arteries perfused in vitro.},
Journal = {Journal of biomechanics},
Volume = {22},
Number = {11-12},
Pages = {1141-50},
Year = {1989},
ISSN = {0021-9290},
Keywords = {Animals • Biomechanics • Carotid Arteries •
Dogs • Endothelium, Vascular • Hemodynamics •
Lipoproteins, LDL • Mathematical Computing •
Models, Cardiovascular • metabolism • physiology*
• physiopathology},
Abstract = {The mathematical model of Hung (Tsai and Hung, 1984) is
employed to determine the wall shear rate acting on canine
carotid arteries perfused in vitro. Model equations for
pulsatile flow in a deformable vessel are coupled with
experimental data of dynamic pressure drop, flow rate,
vessel radius and radial wall motion. Derived quantities,
e.g. velocity profiles and wall shear, are obtained for
vessels exposed to 'normotensive' hemodynamics,
'hypertension' simulations and perfusions in which the
compliance of the vessel wall is deliberately altered. Our
results indicate that wall shear varies markedly as a
function of the hemodynamic environment. The effects of
vessel radius vs flow rate on the development of wall shear
are also demonstrated. It is found that convective processes
correlate with the magnitude of wall shear in the
'hypertension' simulations. The present findings and
complementary published data may explain, at least in part,
the variations in vessel wall transport and endothelial cell
biology we observe as a function of the hemodynamic
environment. For example we have documented that the
exposure of canine carotids to 'hypertensive' (vs
'normotensive') hemodynamics is associated with an increased
flux of lipoproteins (LDL) into the intima and luminal
media. Alternations in wall compliance, on the other hand,
profoundly influence endothelial shape, orientation and
cytoskeletal array.},
Language = {eng},
Key = {fds174149}
}
@booklet{Johnson89,
Author = {G. A. Johnson and T. K. Hung and A. M. Brant and H. S.
Borovetz},
Title = {Experimental-determination of wall shear rate in canine
carotid arteries perfused invitro},
Journal = {Journal Of Biomechanics},
Volume = {22},
Number = {11-12},
Pages = {1141 -- 1150},
Year = {1989},
Key = {Johnson89}
}
@booklet{Johnson98a,
Author = {G. A. Johnson and C. A. Gray and J. B. Word and T. L. Ott and T. E. Spencer and R. C. Burghardt and F. W.
Bazer},
Title = {Expression and secretion of osteopontin by ovine endometrium
and conceptuses.},
Journal = {Biology Of Reproduction},
Volume = {58},
Pages = {119 -- 119},
Year = {1998},
Key = {Johnson98a}
}
@booklet{Pfarrer02,
Author = {C. D. Pfarrer and S. Hallack and G. A. Johnson and R. C.
Burghardt and F. W. Bazer and R. Leiser},
Title = {Expression of osteopontin in bovine placentomes and
interplacentomal areas from early placentation until
term.},
Journal = {Biology Of Reproduction},
Volume = {66},
Pages = {229 -- 230},
Year = {2002},
Key = {Pfarrer02}
}
@article{fds174089,
Author = {MD Ashworth and JW Ross and J Hu and FJ White and DR Stein and U Desilva and GA Johnson and TE Spencer and RD Geisert},
Title = {Expression of porcine endometrial prostaglandin synthase
during the estrous cycle and early pregnancy, and following
endocrine disruption of pregnancy.},
Journal = {Biology of reproduction},
Volume = {74},
Number = {6},
Pages = {1007-15},
Year = {2006},
Month = {June},
ISSN = {0006-3363},
url = {http://dx.doi.org/10.1095/biolreprod.105.046557},
Keywords = {Animals • Embryo Loss • Endometrium •
Estrogens • Estrous Cycle • Female •
Fertilization • Gene Expression Profiling • Gene
Expression Regulation, Developmental • Gene Expression
Regulation, Enzymologic • Interleukin-1 •
Placentation • Pregnancy • Pregnancy, Animal
• Prostaglandin-Endoperoxide Synthases • RNA,
Messenger • Swine • Transcription, Genetic •
analysis • drug effects • drug effects* •
enzymology* • genetics • genetics* •
metabolism* • pharmacology* • physiology •
physiopathology • secretion},
Abstract = {Porcine trophoblast attachment to the uterine surface is
associated with increased conceptus and endometrial
production of prostaglandins. Conceptus secretion of
estrogen on Day 12 of gestation is important for
establishment of pregnancy; however, early (Days 9 and 10)
exposure to exogenous estrogens results in embryonic
mortality. Present studies established the temporal and
spatial pattern of endometrial PTGS1 (prostaglandin-endoperoxide
synthase 1) and PTGS2 expression during the estrous cycle
and early pregnancy and determined the effect of early
estrogen treatment on endometrial PTGS expression in
pregnant gilts. Endometrial PTGS1 mRNA expression increased
2- to 3-fold after Day 10 of the estrous cycle and
pregnancy, whereas PTGS2 mRNA expression increased 76-fold
between Days 5 and 15 of the estrous cycle and pregnancy.
Increased expression of the PTGS2 transcript was detected in
the lumenal epithelium after Day 10 in both cyclic and
pregnant gilts. There was a 10- and 20-fold increase in
endometrial PTGS2 protein expression between Days 5 and 18
of the estrous cycle and pregnancy respectively.
Administration of estrogen on Days 9 and 10 of gestation
increased endometrial PTGS2 mRNA and protein on Day 10, but
decreased PTGS2 mRNA and protein in lumenal epithelium (LE)
on Day 12 of gestation compared to vehicle-treated gilts.
The present study demonstrates that an increase in uterine
epithelial PTGS2 expression occurs after Day 10 of the
estrous cycle and early pregnancy in the pig. The
conceptus-independent increase in the uterine LE indicates
that a novel pathway exists for endometrial induction PTGS2
expression before conceptus elongation and attachment to the
uterine surface. Epithelial expression of PTGS2 may serve as
one of the signals for placental attachment and embryo
survival in the pig. Early administration of estrogen on
Days 9 and 10 of pregnancy alters endometrial PTGS2 mRNA and
protein expression, which may, at least in part, represent a
mechanism by which endocrine disruption of pregnancy causes
total embryonic loss during implantation in the
pig.},
Language = {eng},
Doi = {10.1095/biolreprod.105.046557},
Key = {fds174089}
}
@booklet{Hicks00,
Author = {B. A. Hicks and K. G. Carnahan and J. A. Baldock and S. J.
Yankee and F. W. Bazer and G. A. Johnson and T. E. Spencer and T. L. Ott},
Title = {Expression of the antiviral protein Mx in an immortalized
ovine uterine glandular epithelial cell line.},
Journal = {Biology Of Reproduction},
Volume = {62},
Pages = {288 -- 289},
Year = {2000},
Key = {Hicks00}
}
@booklet{Johnson99f,
Author = {G. A. Johnson and T. E. Spencer and T. R. Hansen and K. J.
Austin and R. C. Burghardt and F. W. Bazer},
Title = {Expression of the interferon tau inducible ubiquitin
cross-reactive protein in the ovine uterus},
Journal = {Biology Of Reproduction},
Volume = {61},
Number = {1},
Pages = {312 -- 318},
Year = {1999},
Month = {July},
Key = {Johnson99f}
}
@article{fds174235,
Author = {GA Johnson and TE Spencer and TR Hansen and KJ Austin and RC Burghardt and FW Bazer},
Title = {Expression of the interferon tau inducible ubiquitin
cross-reactive protein in the ovine uterus.},
Journal = {Biology of reproduction},
Volume = {61},
Number = {1},
Pages = {312-8},
Year = {1999},
Month = {July},
ISSN = {0006-3363},
Keywords = {Animals • Blotting, Northern • Blotting, Western
• Cattle • Endometrium • Epithelium •
Female • Gene Expression Regulation* • Gestational
Age • In Situ Hybridization • Interferon Type I
• Ovariectomy • Pregnancy • Pregnancy
Proteins • RNA, Messenger • Recombinant Proteins
• Sheep* • Stromal Cells • Ubiquitins •
Uterus • analogs & derivatives* • analysis •
chemistry • genetics • metabolism* •
pharmacology • pharmacology*},
Abstract = {Ubiquitin cross-reactive protein (UCRP) is a 17-kDa protein
that shows cross-reactivity with ubiquitin antisera and
retains the carboxyl-terminal Leu-Arg-Gly-Gly amino acid
sequence of ubiquitin that ligates to, and directs
degradation of, cytosolic proteins. It has been reported
that bovine endometrial UCRP is synthesized and secreted in
response to conceptus-derived interferon-tau (IFNtau). In
the present studies, UCRP mRNA and protein were detected in
ovine endometrium. Ovine UCRP mRNA was detectable on Day 13,
peaked at Day 15, and remained high through Day 19 of
pregnancy. The UCRP mRNA was localized to the luminal
epithelium (LE), stromal cells (ST) immediately beneath the
LE, and shallow glandular epithelium (GE) on Day 13, but it
extended to the deep GE, deep ST, and myometrium of uterine
tissues by Day 15 of pregnancy. Western blotting revealed
induction of UCRP in the endometrial extracts from pregnant,
but not cyclic, ewes. Ovine UCRP was also detected in
uterine flushings from Days 15 and 17 of pregnancy and
immunoprecipitated from Day 17 pregnant endometrial
explant-conditioned medium. Treatment of immortalized ovine
LE cells with recombinant ovine (ro) IFNtau induced
cytosolic expression of UCRP, and intrauterine injection of
roIFNtau into ovariectomized cyclic ewes induced endometrial
expression of UCRP mRNA. These results are the first to
describe temporal and spatial alterations in the cellular
localization of UCRP in the ruminant uterus. Collectively,
UCRP is synthesized and secreted by the ovine endometrium in
response to IFNtau during early pregnancy. Because UCRP is
present in the uterus and uterine flushings, it may regulate
endometrial proteins associated with establishment and
maintenance of early pregnancy in ruminants.},
Language = {eng},
Key = {fds174235}
}
@booklet{Hicks03,
Author = {B. A. Hicks and S. J. Etter and K. G. Carnahan and M. M.
Joyce and A. A. Assiri and S. J. Carling and K. Kodali and G. A. Johnson and T. R. Hansen and M. A. Mirando and G. L.
Woods and D. K. Vanderwall and T. L. Ott},
Title = {Expression of the uterine Mx protein in cyclic and pregnant
cows, gilts, and mares},
Journal = {Journal Of Animal Science},
Volume = {81},
Number = {6},
Pages = {1552 -- 1561},
Year = {2003},
Month = {June},
Key = {Hicks03}
}
@article{fds174174,
Author = {BA Hicks and SJ Etter and KG Carnahan and MM Joyce and AA Assiri and SJ
Carling, K Kodali and GA Johnson and TR Hansen and MA Mirando and GL
Woods, DK Vanderwall and TL Ott},
Title = {Expression of the uterine Mx protein in cyclic and pregnant
cows, gilts, and mares.},
Journal = {Journal of animal science},
Volume = {81},
Number = {6},
Pages = {1552-61},
Year = {2003},
Month = {June},
ISSN = {0021-8812},
Keywords = {Animals • Blotting, Northern • Blotting, Western
• Cattle • Estrus • Female • GTP-Binding
Proteins • Gene Expression Regulation • Horses
• In Situ Hybridization • Pregnancy •
Pregnancy, Animal • Swine • Uterus •
biosynthesis* • metabolism • metabolism* •
physiology* • veterinary},
Abstract = {Pregnancy and interferon-tau (IFN tau) upregulate uterine Mx
gene expression in ewes; however, the only known role for Mx
is in the immune response to viral infection. We hypothesize
that Mx functions as a conceptus-induced component of the
anti-luteolytic mechanism and/or regulator of endometrial
secretion or uterine remodeling during early pregnancy. This
study was conducted to determine the effects of early
pregnancy on uterine Mx expression in domestic farm species
with varied mechanisms of pregnancy recognition. Endometrium
from cows, gilts, and mares was collected during the first
20 d of the estrous cycle or pregnancy, and total messenger
RNA (mRNA) and protein were analyzed for steady-state levels
of Mx mRNA and protein. Northern blot analysis of Mx mRNA
detected an approximately 2.5 Kb of mRNA in endometrium from
each species. In pregnant cows, steady-state levels of Mx
mRNA increased 10-fold (P < 0.05) above levels observed in
cyclic cows by d 15 to 18. In cyclic gilts, slot blot
analysis indicated that endometrial Mx mRNA levels did not
change between d 5 and 18 of the cycle. However, in pregnant
gilts, Mx levels tended (P = 0.06) to be elevated two-fold
on d 16 only, and in situ hybridization indicated that this
increase occurred in the stroma. In mares, Mx mRNA was low,
but detectable, and did not change between ovulation (d 0)
and d 20, regardless of reproductive status. Western blot
analysis revealed multiple immunoreactive Mx protein bands
in each species. One band was specific to pregnancy in cows.
As in ewes, in situ hybridization analysis indicated that Mx
mRNA was strongly expressed in the luminal epithelium,
stroma, and myometrium by d 18 in cows. However, on d 14 in
gilts, Mx was expressed primarily in the stroma, and on d 14
in mares, low levels of Mx expression were confined largely
to the luminal epithelium. The uteruses of cows, gilts, and
mares express Mx, and expression is upregulated during
pregnancy in cows and gilts--animals whose conceptuses
secrete interferons during early pregnancy, but that possess
different mechanisms for pregnancy recognition.},
Language = {eng},
Key = {fds174174}
}
@booklet{Spencer97,
Author = {T. A. Spencer and D. S. Clark and G. A. Johnson and S. K.
Erickson and L. K. Curtiss},
Title = {Feasibility of an immunoassay for mevalonolactone},
Journal = {Bioorganic \& Medicinal Chemistry},
Volume = {5},
Number = {5},
Pages = {873 -- 882},
Year = {1997},
Month = {May},
Key = {Spencer97}
}
@article{fds174267,
Author = {TA Spencer and DS Clark and GA Johnson and SK Erickson and LK
Curtiss},
Title = {Feasibility of an immunoassay for mevalonolactone.},
Journal = {Bioorganic & medicinal chemistry},
Volume = {5},
Number = {5},
Pages = {873-82},
Year = {1997},
Month = {May},
ISSN = {0968-0896},
Keywords = {Animals • Antibody Formation • Haptens •
Hemocyanin • Hydrogen-Ion Concentration •
Immunoassay • Mevalonic Acid • Rabbits •
Stereoisomerism • analogs & derivatives* •
analysis • chemistry • immunology •
methods*},
Abstract = {Mevalonic acid is a key intermediate in a broad spectrum of
cellular biological processes and their regulation.
Availability of a rapid, sensitive and accurate method for
its assay would be highly useful. Therefore, the feasibility
of developing an immunoassay for mevalonic acid in
biological samples was explored. The strategy employed was
to synthesize several racemic haptens structurally
resembling R-mevalonolactone, the cyclic form of mevalonic
acid present at lower pH and presumed to be more antigenic.
Two of these haptens were coupled to keyhole limpet
hemocyanin, and the resulting conjugates were used
successfully to generate antibodies in rabbits. The first
antiserum bound to R,S-mevalonolactone much more effectively
at pH 4.0 than at pH 6.0, consistent with the structural
resemblance of the haptens to the lactone form. This
antiserum also bound the free hapten from which it was
generated and two others of different structure with
comparable effectiveness; and slightly better than it bound
R,S-mevalonolactone at pH 4.0. Similar results were obtained
with the antiserum to the second hapten. The binding of
either antiserum to the natural enantiomer,
R-mevalonolactone, was 20 times weaker than to
R,S-mevalonolactone, suggesting that the nonbiological
enantiomer was more antigenic. Nevertheless, the results
demonstrate that an immunochemical approach to accurate
quantitation of mevalonic acid in biological samples is
feasible.},
Language = {eng},
Key = {fds174267}
}
@article{fds174202,
Author = {TE Spencer and GA Johnson and FW Bazer and RC Burghardt},
Title = {Fetal-maternal interactions during the establishment of
pregnancy in ruminants.},
Journal = {Society of Reproduction and Fertility supplement},
Volume = {64},
Pages = {379-96},
Year = {2007},
Keywords = {Animals • Blastocyst • Embryo Implantation •
Embryonic Development • Endometrium • Female
• Hormones • Luteolysis • Pregnancy •
Sheep • metabolism* • physiology •
physiology*},
Abstract = {This review integrates established information with new
insights into molecular and physiological mechanisms
responsible for events leading to pregnancy recognition,
endometrial receptivity, and implantation with emphasis on
sheep. After formation of the corpus luteum, progesterone
acts on the endometrium and stimulates blastocyst growth and
elongation to form a filamentous conceptus (embryo/fetus and
associated extraembryonic membranes). Recurrent early
pregnancy loss in the uterine gland knockout ewe model
indicates that endometrial epithelial secretions are
essential for peri-implantation blastocyst survival and
growth. The elongating sheep conceptus secretes interferon
tau (IFNT) that acts on the endometrium to inhibit
development of the luteolytic mechanism by inhibiting
transcription of the estrogen receptor alpha (ESR1) gene in
the luminal (LE) and superficial ductal glandular (sGE)
epithelia, which prevents estrogen-induction of oxytocin
receptors (OXTR) and production of luteolytic prostaglandin
F2-alpha pulses. Progesterone downregulates its receptors
(PGR) in LE and then GE, correlating with a reduction of
anti-adhesive MUC1 (mucin glycoprotein one) and induction of
secreted LGALS15 (galectin 15) and SPP1 (secreted
phosphoprotein one), that are proposed to regulate
trophectoderm growth and adhesion. IFNT acts on the LE to
induce WNT7A (wingless-type MMTV integration site family
member 7A) and to stimulate LGALS15, CTSL (cathepsin L), and
CST3 (cystatin C), which may regulate conceptus development
and implantation. During the peri-implantation period,
trophoblast giant binucleate cells (BNC) begin to
differentiate from mononuclear trophectoderm cells, migrate
and then fuse with the uterine LE as well as each other to
form multinucleated syncytial plaques. Trophoblast giant BNC
secrete chorionic somatomammotropin (CSH1 or placental
lactogen) that acts on the endometrial glands to stimulate
their morphogenesis and differentiated function. The
interactive, coordinated and stage-specific effects of
ovarian and placental hormones regulate endometrial events
necessary for fetal-maternal interactions and successful
establishment of pregnancy.},
Language = {eng},
Key = {fds174202}
}
@booklet{Jefferies01,
Author = {B. Jefferies and G. A. Johnson},
Title = {Feynman's operational calculi for noncommuting operators:
Definitions and elementary properties},
Journal = {Russian Journal Of Mathematical Physics},
Volume = {8},
Number = {2},
Pages = {153 -- 171},
Year = {2001},
Key = {Jefferies01}
}
@booklet{Johnson00a,
Author = {G. A. Johnson and M. D. Todd and B. L. Althouse and C. C.
Chang},
Title = {Fiber Bragg grating interrogation and multiplexing with a 3
x 3 coupler and a scanning filter},
Journal = {Journal Of Lightwave Technology},
Volume = {18},
Number = {8},
Pages = {1101 -- 1105},
Year = {2000},
Month = {August},
Key = {Johnson00a}
}
@booklet{Brau02,
Author = {A. C. S. Brau and C. T. Wheeler and L. W. Hedlund and G. A.
Johnson},
Title = {Fiber-optic stethoscope: A cardiac monitoring and gating
system for magnetic resonance microscopy},
Journal = {Magnetic Resonance In Medicine},
Volume = {47},
Number = {2},
Pages = {314 -- 321},
Year = {2002},
Month = {February},
Key = {Brau02}
}
@article{fds269015,
Author = {Brau, ACS and Wheeler, CT and Hedlund, LW and Johnson,
GA},
Title = {Fiber-optic stethoscope: a cardiac monitoring and gating
system for magnetic resonance microscopy.},
Journal = {Magn Reson Med},
Volume = {47},
Number = {2},
Pages = {314-321},
Year = {2002},
Month = {February},
ISSN = {0740-3194},
url = {http://www.ncbi.nlm.nih.gov/pubmed/11810675},
Keywords = {Animals • Electrocardiography • Esophagoscopy*
• Fiber Optics* • Heart Atria • Heart Rate
• Heart Ventricles • Hemodynamic Processes •
Magnetic Resonance Imaging • Mice • Mice, Inbred
C57BL • Microscopy • Myocardial Contraction •
Rats • Stethoscopes* • anatomy & histology* •
instrumentation* • physiology},
Abstract = {A fundamental problem associated with using the conventional
electrocardiograph (ECG) to monitor a subject's cardiac
activity during magnetic resonance imaging (MRI) is the
distortion of the ECG due to electromagnetic interference.
This problem is particularly pronounced in MR microscopy
(MRI of small animals at microscopic resolutions (< 0.03
mm(3))) because the strong, rapidly-switching magnetic field
gradients induce artifacts in the animal's ECG that often
mimic electrophysiologic activity, impairing the use of the
ECG for cardiac monitoring and gating purposes. The
fiber-optic stethoscope system offers a novel approach to
measuring cardiac activity that, unlike the ECG, is immune
to electromagnetic effects. The fiber-optic stethoscope is
perorally inserted into the esophagus of small animals to
optically detect pulsatile compression of the esophageal
wall. The optical system is shown to provide a robust
cardiac monitoring and gating signal in rats and mice during
routine cardiac MR microscopy.},
Doi = {10.1002/mrm.10049},
Key = {fds269015}
}
@booklet{Sherrier88,
Author = {Sherrier, RH and Suddarth, SA and Johnson, GA},
Title = {Film-Based Digital Tomosynthesis Of The Chest},
Journal = {Optical Engineering},
Volume = {27},
Number = {8},
Pages = {691-695},
Publisher = {SPIE-Intl Soc Optical Eng},
Year = {1988},
Month = {August},
ISSN = {0091-3286},
url = {http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:A1988P713200014&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=47d3190e77e5a3a53558812f597b0b92},
Doi = {10.1117/12.7976742},
Key = {Sherrier88}
}
@booklet{Todd98a,
Author = {M. D. Todd and G. A. Johnson and B. A. Althouse and S. T.
Vohra},
Title = {Flexural beam-based fiber Bragg grating accelerometers},
Journal = {Ieee Photonics Technology Letters},
Volume = {10},
Number = {11},
Pages = {1605 -- 1607},
Year = {1998},
Month = {November},
Key = {Todd98a}
}
@booklet{Todd98,
Author = {M. D. Todd and G. A. Johnson and B. A. Althouse and S. T.
Vohra},
Title = {Flexural beam-based fiber Bragg grating accelerometers (vol
10, pg 1605, 1998)},
Journal = {Ieee Photonics Technology Letters},
Volume = {10},
Number = {12},
Pages = {1799 -- 1799},
Year = {1998},
Month = {December},
Key = {Todd98}
}
@booklet{Croft97,
Author = {P. J. Croft and R. L. Pfost and J. M. Medlin and G. A.
Johnson},
Title = {Fog forecasting for the southern region: A conceptual model
approach},
Journal = {Weather And Forecasting},
Volume = {12},
Number = {3},
Pages = {545 -- 556},
Year = {1997},
Month = {September},
Key = {Croft97}
}
@article{fds268866,
Author = {Bucholz, E and Ghaghada, K and Qi, Y and Mukundan, S and Johnson,
GA},
Title = {Four-dimensional MR microscopy of the mouse heart using
radial acquisition and liposomal gadolinium contrast
agent.},
Journal = {Magn Reson Med},
Volume = {60},
Number = {1},
Pages = {111-118},
Year = {2008},
Month = {July},
ISSN = {0740-3194},
url = {http://www.ncbi.nlm.nih.gov/pubmed/18581419},
Keywords = {Animals • Contrast Media • Gadolinium • Heart
• Liposomes • Magnetic Resonance Imaging •
Mice • Microscopy • anatomy & histology •
diagnostic use • methods • methods* •
physiology*},
Abstract = {Magnetic resonance microscopy (MRM) has become an important
tool for small animal cardiac imaging. In relation to
competing technologies (microCT and ultrasound), MR is
limited by spatial resolution, temporal resolution, and
acquisition time. All three of these limitations have been
addressed by developing a four-dimensional (4D) (3D plus
time) radial acquisition (RA) sequence. The signal-to-noise
ratio (SNR) has been optimized by minimizing the echo time
(TE) (300 us). The temporal resolution and throughput have
been improved by center-out trajectories resulting in
repetition time (TR) <2.5 ms. The contrast has been enhanced
through the use of a liposomal blood pool agent that reduces
the T(1) of the blood to <400 ms. We have developed
protocols for three specific applications: 1)
high-throughput with spatial resolution of 87 x 87 x 352
um(3) (voxel volume = 2.7 nL) and acquisition time of 16
min; 2) high-temporal resolution with spatial resolution of
87 x 87 x 352 um(3) (voxel volume = 2.7 nL) and temporal
resolution at 4.8 ms and acquisition time of 32 minutes; and
3) high-resolution isotropic imaging at 87 x 87 x 87 um(3)
(voxel volume = 0.68 nL) and acquisition time of 31 min. The
4D image arrays allow direct measure of cardiac functional
parameters dependent on chamber volumes, e.g., ejection
fraction (EF), end diastolic volume (EDV), and end systolic
volume (ESV).},
Doi = {10.1002/mrm.21618},
Key = {fds268866}
}
@article{fds268710,
Author = {Xie, L and Subashi, E and Qi, Y and Knepper, MA and Johnson,
GA},
Title = {Four-dimensional MRI of renal function in the developing
mouse.},
Journal = {NMR Biomed},
Volume = {27},
Number = {9},
Pages = {1094-1102},
Year = {2014},
Month = {September},
ISSN = {0952-3480},
url = {http://dx.doi.org/10.1002/nbm.3162},
Abstract = {The major roles of filtration, metabolism and high blood
flow make the kidney highly vulnerable to drug-induced
toxicity and other renal injuries. A method to follow kidney
function is essential for the early screening of toxicity
and malformations. In this study, we acquired high
spatiotemporal resolution (four dimensional) datasets of
normal mice to follow changes in kidney structure and
function during development. The data were acquired with
dynamic contrast-enhanced MRI (via keyhole imaging) and a
cryogenic surface coil, allowing us to obtain a full
three-dimensional image (isotropic resolution,
125 microns) every 7.7 s over a 50-min scan. This time
course permitted the demonstration of both contrast
enhancement and clearance. Functional changes were measured
over a 17-week course (at 3, 5, 7, 9, 13 and 17 weeks).
The time dimension of the MRI dataset was processed to
produce unique image contrasts to segment the four regions
of the kidney: cortex (CO), outer stripe (OS) of the outer
medulla (OM), inner stripe (IS) of the OM and inner medulla
(IM). Local volumes, time-to-peak (TTP) values and decay
constants (DC) were measured in each renal region. These
metrics increased significantly with age, with the exception
of DC values in the IS and OS. These data will serve as a
foundation for studies of normal renal physiology and future
studies of renal diseases that require early detection and
intervention.},
Doi = {10.1002/nbm.3162},
Key = {fds268710}
}
@article{fds292760,
Author = {Johnson, GA},
Title = {Free radicals formed by exposure of pyrimidine solids to
sodium atoms: an electron spin resonance
study.},
Journal = {Proc Natl Acad Sci U S A},
Volume = {72},
Number = {3},
Pages = {974-978},
Year = {1975},
Month = {March},
ISSN = {0027-8424},
url = {http://www.ncbi.nlm.nih.gov/pubmed/165496},
Keywords = {Alloxan • Bromouracil • Cytosine • Electron
Spin Resonance Spectroscopy • Fluorouracil • Free
Radicals • Pyrimidines* • Sodium* • Thymine
• Uracil • analogs & derivatives},
Abstract = {Sodium atoms have been deposited on various pyrimidine
powders under high vacuum at 77 degrees K and the electron
spin resonance spectra of the resulting free radicals have
been observed. Generally, the spectra show that the electron
of the Na goes into a molecular orbital of the pyrimidine
ring and the Na+ ions become attached to a carbonyl oxygen
of the resulting pyrimidine anion. In 5-fluorouracil and
5-chlorouracil, however, the halogen is evidently abstracted
by the Na to form NaF or NaC1 and the neutral uracil
radical. Thymine shows evidence for H-addition radicals as
well as the Na+-[thymine]-complex. The H source for the
addition radicals may be an H-2-0 impurity in the sample,
with which the Na atoms combine to release the H atoms. In
addition to a resonance with g equals 2.00 from the
pyrimidine anion radical, broad resonances with g greater
than 2 were observed for 5-bromouracil, 5-chlorouracil and
5-iodouracil, as well as for alloxan and cytosine. These
resonances, generally unstable at room temperature, are
believed to arise from electrons trapped in interstitial
sties or vacancies in the lattice.},
Language = {eng},
Doi = {10.1073/pnas.72.3.974},
Key = {fds292760}
}
@article{fds268816,
Author = {Zhang, X and Badea, CT and Hood, G and Wetzel, AW and Stiles, JR and Johnson, GA},
Title = {Free-space fluorescence tomography with adaptive sampling
based on anatomical information from microCT.},
Journal = {Proc SPIE Int Soc Opt Eng},
Volume = {7757},
Number = {775706},
Year = {2010},
ISSN = {0277-786X},
url = {http://www.ncbi.nlm.nih.gov/pubmed/21743784},
Abstract = {Image reconstruction is one of the main challenges for
fluorescence tomography. For in vivo experiments on small
animals, in particular, the inhomogeneous optical properties
and irregular surface of the animal make free-space image
reconstruction challenging because of the difficulties in
accurately modeling the forward problem and the finite
dynamic range of the photodetector. These two factors are
fundamentally limited by the currently available forward
models and photonic technologies. Nonetheless, both
limitations can be significantly eased using a signal
processing approach. We have recently constructed a
free-space panoramic fluorescence diffuse optical tomography
system to take advantage of co-registered microCT data
acquired from the same animal. In this article, we present a
data processing strategy that adaptively selects the optical
sampling points in the raw 2-D fluorescent CCD images.
Specifically, the general sampling area and sampling density
are initially specified to create a set of potential
sampling points sufficient to cover the region of interest.
Based on 3-D anatomical information from the microCT and the
fluorescent CCD images, data points are excluded from the
set when they are located in an area where either the
forward model is known to be problematic (e.g., large
wrinkles on the skin) or where the signal is unreliable
(e.g., saturated or low signal-to-noise ratio). Parallel
Monte Carlo software was implemented to compute the
sensitivity function for image reconstruction. Animal
experiments were conducted on a mouse cadaver with an
artificial fluorescent inclusion. Compared to our previous
results using a finite element method, the newly developed
parallel Monte Carlo software and the adaptive sampling
strategy produced favorable reconstruction
results.},
Language = {ENG},
Doi = {10.1117/12.841891},
Key = {fds268816}
}
@booklet{Johnson85d,
Author = {G. A. Johnson},
Title = {From constant air to variable},
Journal = {Ashrae Journal-american Society Of Heating Refrigerating And
Air-conditioning Engineers},
Volume = {27},
Number = {1},
Pages = {106 -- \&},
Year = {1985},
Key = {Johnson85d}
}
@booklet{Jaeger00a,
Author = {L. A. Jaeger and L. S. Bustamante and G. A. Johnson and F.
W. Bazer and R. C. Burghardt},
Title = {Functional activation of conceptus and maternal
integrins},
Journal = {Faseb Journal},
Volume = {14},
Number = {4},
Pages = {A783 -- A783},
Year = {2000},
Month = {March},
Key = {Jaeger00a}
}
@article{fds174181,
Author = {LA Jaeger and GA Johnson and H Ka and JG Garlow and RC Burghardt and TE
Spencer, FW Bazer},
Title = {Functional analysis of autocrine and paracrine signalling at
the uterine-conceptus interface in pigs.},
Journal = {Reproduction (Cambridge, England) Supplement},
Volume = {58},
Pages = {191-207},
Year = {2001},
ISSN = {1477-0415},
Keywords = {Animals • Autocrine Communication • Blastocyst
• Cell Communication • Cytokines • Embryo
Implantation • Estrogens • Extracellular Matrix
Proteins • Female • Growth Substances •
Integrins • Paracrine Communication • Pregnancy
• Progesterone • Receptors, Estrogen •
Receptors, Progesterone • Swine • Uterus •
metabolism • metabolism* • physiology •
physiology*},
Abstract = {The complexity of implantation necessitates intimate
dialogue between conceptus and maternal cells, and precise
coordination of maternal and conceptus signalling events.
Maternal and conceptus-derived steroid hormones, growth
factors and cytokines, as well as integrins and their
ligands, have important and inter-related roles in mediating
adhesion between apical aspects of conceptus trophectoderm
and maternal uterine luminal epithelium that leads to
formation of an epitheliochorial placenta. Integrin
receptors appear to play fundamental roles in the
implantation cascade and may interact with extracellular
matrix molecules and other ligands to transduce cellular
signals through autocrine and paracrine mechanisms.
Functional in vitro analyses can be used to monitor
individual contributions of specific integrin receptors and
ligands to the signalling cascades of the maternal-conceptus
interface. Integrative studies of implantation in pigs,
using in vivo and in vitro approaches, are required to
understand conceptus attachment and implantation in this
species, and provide valuable opportunities to understand
the fundamental mechanisms of implantation in all
species.},
Language = {eng},
Key = {fds174181}
}
@article{fds174096,
Author = {LA Jaeger and AK Spiegel and NH Ing and GA Johnson and FW Bazer and RC
Burghardt},
Title = {Functional effects of transforming growth factor beta on
adhesive properties of porcine trophectoderm.},
Journal = {Endocrinology},
Volume = {146},
Number = {9},
Pages = {3933-42},
Year = {2005},
Month = {September},
ISSN = {0013-7227},
url = {http://dx.doi.org/10.1210/en.2005-0090},
Keywords = {Animals • Blastocyst • Cell Adhesion • Cell
Line, Transformed • Embryo Implantation •
Endometrium • Female • Fibronectins •
Integrins • Oligopeptides • RNA, Messenger •
Signal Transduction • Swine • Talin •
Transforming Growth Factor beta • Transforming Growth
Factor beta1 • analysis • cytology* •
genetics • metabolism • metabolism* •
pharmacology • physiology • physiology*},
Abstract = {In pigs, expression and amounts of biologically active
TGFbetas at the conceptus-maternal interface increase
significantly as conceptuses elongate and begin the
implantation process. Before their activation, secreted
TGFbetas are noncovalently associated with their respective,
isoform-specific latency-associated peptides (LAPs), which
contain the Arg-Gly-Asp (RGD) amino acid sequence that
serves as a ligand for numerous integrins. Objectives of
this study were to determine whether TGFbeta1 increases
production of fibronectin by porcine trophectoderm, whether
porcine trophectoderm adheres specifically to fibronectin
and LAP, and whether functional interactions between porcine
trophectoderm and the two TGFbeta-associated proteins,
fibronectin and LAP, are integrin mediated. Porcine
trophectoderm cells (pTr2) were cultured in presence of
TGFbeta1, LAP, or pan-neutralizing anti-TGFbeta antibody;
TGFbeta specifically increased (P < 0.05) fibronectin mRNA
levels, as determined by Northern and slot blot analyses.
Immunofluorescence microscopy demonstrated a TGFbeta-induced
increase in fibronectin in pTr2 cells. In dispersed cell
adhesion assays, adhesion of pTr2 cells to fibronectin was
inhibited by an RGD-containing peptide (P < 0.05) and pTr2
cells attached to recombinant LAP but not to an LAP mutant,
which contained an RGE sequence rather than the RGD site (P
< 0.05). Fibronectin- and LAP-coated microbeads induced
integrin activation at apical surfaces of both trophectoderm
and uterine luminal epithelial cells, as indicated by
aggregation and transmembrane accumulation of talin detected
with immunofluorescence microscopy. Cell surface
biotinylation and immunoprecipitation revealed integrin
subunits alphav and beta1 on apical membranes of pTr2 cells.
These results suggest multiple effects of TGFbeta at the
porcine conceptus-maternal interface, including
integrin-mediated conceptus-maternal communication through
LAP.},
Language = {eng},
Doi = {10.1210/en.2005-0090},
Key = {fds174096}
}
@article{064610243504,
Author = {Badea, CT and Hedlund, LW and Lin, MD and Boslego, JF and Johnson,
GA},
Title = {Functional imaging in small animals using tomographic
digital subtraction angiography},
Journal = {2006 3rd IEEE International Symposium on Biomedical Imaging:
From Nano to Macro - Proceedings},
Volume = {2006},
Pages = {1208-1211},
Address = {Arlington, VA, United States},
Year = {2006},
Month = {November},
ISBN = {9780780395770},
Keywords = {Imaging systems;Angiography;Computerized tomography;Optical
resolving power;Algorithms;Data structures;},
Abstract = {We propose the use of Tomographic Digital Subtraction
Angiography (TDSA) for functional imaging in small animals.
TDSA combines the advantages of high temporal resolution of
digital subtraction angiography (DSA) and high spatial
resolution of micro-computed tomography (CT). TDSA augments
projection imaging methods such as DSA by providing
three-dimensional information using tomosynthesis or CT
reconstruction algorithms. Thus, four-dimensional (4D)
datasets with a temporal resolution on the order of 100 ms
and spatial resolution ranging from 100 microns to 1 mm,
depending on the scanning angle, can be obtained. The
approach is based on the novel paradigm that the same time
density curves can be reproduced in a number of consecutive
injections of microL volumes of contrast at a series of
different angles of rotation. © 2006 IEEE.},
Key = {064610243504}
}
@article{9054429,
Author = {Badea, C.T. and Hedlund, L.W. and Ming De Lin and Boslego,
J.F. and Johnson, G.A.},
Title = {Functional imaging in small animals using tomographic
digital subtraction angiography},
Journal = {2006 3rd IEEE International Symposium on Biomedical Imaging:
Macro to Nano (IEEE Cat. No.06EX1231C)},
Pages = {1208 - 11},
Address = {Arlington, VA, USA},
Year = {2006},
Keywords = {computerised tomography;diagnostic radiography;image
reconstruction;image resolution;medical image
processing;},
Abstract = {We propose the use of tomographic digital subtraction
angiography (TDSA) for functional imaging in small animals.
TDSA combines the advantages of high temporal resolution of
digital subtraction angiography (DSA) and high spatial
resolution of micro-computed tomography (CT). TDSA augments
projection imaging methods such as DSA by providing
three-dimensional information using tomosynthesis or CT
reconstruction algorithms. Thus, four-dimensional (4D)
datasets with a temporal resolution on the order of 100 ms
and spatial resolution ranging from 100 microns to 1 mm,
depending on the scanning angle, can be obtained. The
approach is based on the novel paradigm that the same time
density curves can be reproduced in a number of consecutive
injections of microL volumes of contrast at a series of
different angles of rotation},
Key = {9054429}
}
@article{fds132766,
Author = {GA Johnson and LW Hedlund},
Title = {Functional imaging of the lung.},
Journal = {Nature medicine, UNITED STATES},
Volume = {2},
Number = {11},
Pages = {1192},
Year = {1996},
Month = {November},
ISSN = {1078-8956},
Keywords = {Lung • Magnetic Resonance Imaging* •
physiology*},
Key = {fds132766}
}
@booklet{Johnson96,
Author = {Johnson, GA and Hedlund, LW},
Title = {Functional imaging of the lung.},
Journal = {Nat Med},
Volume = {2},
Number = {11},
Pages = {1192},
Year = {1996},
Month = {November},
ISSN = {1078-8956},
url = {http://www.ncbi.nlm.nih.gov/pubmed/8898741},
Doi = {10.1038/nm1196-1192},
Key = {Johnson96}
}
@article{fds268976,
Author = {Viallon, M and Cofer, GP and Suddarth, SA and Möller, HE and Chen, XJ and Chawla, MS and Hedlund, LW and Crémillieux, Y and Johnson,
GA},
Title = {Functional MR microscopy of the lung using hyperpolarized
3He.},
Journal = {Magn Reson Med},
Volume = {41},
Number = {4},
Pages = {787-792},
Year = {1999},
Month = {April},
ISSN = {0740-3194},
url = {http://www.ncbi.nlm.nih.gov/pubmed/10332855},
Keywords = {Animals • Guinea Pigs • Helium • Isotopes
• Lung • Magnetic Resonance Imaging, Cine •
Microscopy • cytology • diagnostic use* •
methods • methods* • physiology*},
Abstract = {A new strategy designed to provide functional magnetic
resonance images of the lung in small animals at microscopic
resolution using hyperpolarized 3He is described. The pulse
sequence is based on a combination of radial acquisition
(RA) and CINE techniques, referred to as RA-CINE, and is
designed for use with hyperpolarized 3He to explore lung
ventilation with high temporal and spatial resolution in
small animal models. Ventilation of the live guinea pig is
demonstrated with effective temporal resolution of 50 msec
and in-plane spatial resolution of <100 microm using
hyperpolarized 3He. The RA-CINE sequence allows one to
follow gas inflow and outflow in the airways as well as in
the distal part of the lungs. Regional analysis of signal
intensity variations can be performed and can help assess
functional lung parameters such as residual gas volume and
lung compliance to gas inflow.},
Doi = {10.1002/(sici)1522-2594(199904)41:4<787::aid-mrm18>3.0.co;2-},
Key = {fds268976}
}
@booklet{Viallon99,
Author = {M. Viallon and G. P. Cofer and S. A. Suddarth and H. E.
Moller and X. J. Chen and M. S. Chawla and L. W. Hedlund and Y. Cremillieux and G. A. Johnson},
Title = {Functional MR microscopy of the lung using hyperpolarized
He-3},
Journal = {Magnetic Resonance In Medicine},
Volume = {41},
Number = {4},
Pages = {787 -- 792},
Year = {1999},
Month = {April},
Key = {Viallon99}
}
@article{fds319489,
Author = {Howles, GP and Qi, Y and Rosenzweig, SJ and Nightingale, KR and Johnson,
GA},
Title = {Functional neuroimaging using ultrasonic blood-brain barrier
disruption and manganese-enhanced MRI.},
Journal = {Journal of visualized experiments : JoVE},
Number = {65},
Pages = {e4055},
Year = {2012},
Month = {October},
url = {http://dx.doi.org/10.3791/4055},
Abstract = {Although mice are the dominant model system for studying the
genetic and molecular underpinnings of neuroscience,
functional neuroimaging in mice remains technically
challenging. One approach, Activation-Induced
Manganese-enhanced MRI (AIM MRI), has been used successfully
to map neuronal activity in rodents. In AIM MRI, Mn(2+) acts
a calcium analog and accumulates in depolarized neurons.
Because Mn(2+) shortens the T1 tissue property, regions of
elevated neuronal activity will enhance in MRI. Furthermore,
Mn(2+) clears slowly from the activated regions; therefore,
stimulation can be performed outside the magnet prior to
imaging, enabling greater experimental flexibility. However,
because Mn(2+) does not readily cross the blood-brain
barrier (BBB), the need to open the BBB has limited the use
of AIM MRI, especially in mice. One tool for opening the BBB
is ultrasound. Though potentially damaging, if ultrasound is
administered in combination with gas-filled microbubbles
(i.e., ultrasound contrast agents), the acoustic pressure
required for BBB opening is considerably lower. This
combination of ultrasound and microbubbles can be used to
reliably open the BBB without causing tissue damage. Here, a
method is presented for performing AIM MRI by using
microbubbles and ultrasound to open the BBB. After an
intravenous injection of perflutren microbubbles, an
unfocused pulsed ultrasound beam is applied to the shaved
mouse head for 3 minutes. For simplicity, we refer to this
technique of BBB Opening with Microbubbles and UltraSound as
BOMUS. Using BOMUS to open the BBB throughout both cerebral
hemispheres, manganese is administered to the whole mouse
brain. After experimental stimulation of the lightly sedated
mice, AIM MRI is used to map the neuronal response. To
demonstrate this approach, herein BOMUS and AIM MRI are used
to map unilateral mechanical stimulation of the vibrissae in
lightly sedated mice. Because BOMUS can open the BBB
throughout both hemispheres, the unstimulated side of the
brain is used to control for nonspecific background
stimulation. The resultant 3D activation map agrees well
with published representations of the vibrissae regions of
the barrel field cortex. The ultrasonic opening of the BBB
is fast, noninvasive, and reversible; and thus this approach
is suitable for high-throughput and/or longitudinal studies
in awake mice.},
Doi = {10.3791/4055},
Key = {fds319489}
}
@article{fds268772,
Author = {Howles, GP and Qi, Y and Rosenzweig, SJ and Nightingale, KR and Johnson,
GA},
Title = {Functional neuroimaging using ultrasonic blood-brain barrier
disruption and manganese-enhanced MRI.},
Journal = {J Vis Exp},
Number = {65},
Pages = {e4055},
Year = {2012},
Month = {July},
ISSN = {1940-087X},
url = {http://dx.doi.org/10.3791/4055},
Abstract = {Although mice are the dominant model system for studying the
genetic and molecular underpinnings of neuroscience,
functional neuroimaging in mice remains technically
challenging. One approach, Activation-Induced
Manganese-enhanced MRI (AIM MRI), has been used successfully
to map neuronal activity in rodents. In AIM MRI, Mn(2+) acts
a calcium analog and accumulates in depolarized neurons.
Because Mn(2+) shortens the T1 tissue property, regions of
elevated neuronal activity will enhance in MRI. Furthermore,
Mn(2+) clears slowly from the activated regions; therefore,
stimulation can be performed outside the magnet prior to
imaging, enabling greater experimental flexibility. However,
because Mn(2+) does not readily cross the blood-brain
barrier (BBB), the need to open the BBB has limited the use
of AIM MRI, especially in mice. One tool for opening the BBB
is ultrasound. Though potentially damaging, if ultrasound is
administered in combination with gas-filled microbubbles
(i.e., ultrasound contrast agents), the acoustic pressure
required for BBB opening is considerably lower. This
combination of ultrasound and microbubbles can be used to
reliably open the BBB without causing tissue damage. Here, a
method is presented for performing AIM MRI by using
microbubbles and ultrasound to open the BBB. After an
intravenous injection of perflutren microbubbles, an
unfocused pulsed ultrasound beam is applied to the shaved
mouse head for 3 minutes. For simplicity, we refer to this
technique of BBB Opening with Microbubbles and UltraSound as
BOMUS. Using BOMUS to open the BBB throughout both cerebral
hemispheres, manganese is administered to the whole mouse
brain. After experimental stimulation of the lightly sedated
mice, AIM MRI is used to map the neuronal response. To
demonstrate this approach, herein BOMUS and AIM MRI are used
to map unilateral mechanical stimulation of the vibrissae in
lightly sedated mice. Because BOMUS can open the BBB
throughout both hemispheres, the unstimulated side of the
brain is used to control for nonspecific background
stimulation. The resultant 3D activation map agrees well
with published representations of the vibrissae regions of
the barrel field cortex. The ultrasonic opening of the BBB
is fast, noninvasive, and reversible; and thus this approach
is suitable for high-throughput and/or longitudinal studies
in awake mice.},
Doi = {10.3791/4055},
Key = {fds268772}
}
@booklet{Chen04a,
Author = {Chen, BT and Yordanov, AT and Johnson, GA},
Title = {Functional pulmonary MR microscopy in mice},
Journal = {FASEB JOURNAL},
Volume = {18},
Number = {5},
Pages = {A785-A785},
Publisher = {FEDERATION AMER SOC EXP BIOL},
Year = {2004},
Month = {March},
ISSN = {0892-6638},
url = {http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000220470700119&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=47d3190e77e5a3a53558812f597b0b92},
Key = {Chen04a}
}
@booklet{Pecora97,
Author = {L. M. Pecora and T. L. Carroll and G. A. Johnson and D. J.
Mar and J. F. Heagy},
Title = {Fundamentals of synchronization in chaotic systems,
concepts, and applications},
Journal = {Chaos},
Volume = {7},
Number = {4},
Pages = {520 -- 543},
Year = {1997},
Month = {December},
Key = {Pecora97}
}
@article{fds174115,
Author = {LM Pecora and TL Carroll and GA Johnson and DJ Mar and JF
Heagy},
Title = {Fundamentals of synchronization in chaotic systems,
concepts, and applications.},
Journal = {Chaos (Woodbury, N.Y.)},
Volume = {7},
Number = {4},
Pages = {520-543},
Year = {1997},
Month = {December},
ISSN = {1089-7682},
url = {http://dx.doi.org/10.1063/1.166278},
Abstract = {The field of chaotic synchronization has grown considerably
since its advent in 1990. Several subdisciplines and
"cottage industries" have emerged that have taken on bona
fide lives of their own. Our purpose in this paper is to
collect results from these various areas in a review article
format with a tutorial emphasis. Fundamentals of chaotic
synchronization are reviewed first with emphases on the
geometry of synchronization and stability criteria. Several
widely used coupling configurations are examined and, when
available, experimental demonstrations of their success
(generally with chaotic circuit systems) are described.
Particular focus is given to the recent notion of
synchronous substitution-a method to synchronize chaotic
systems using a larger class of scalar chaotic coupling
signals than previously thought possible. Connections
between this technique and well-known control theory results
are also outlined. Extensions of the technique are presented
that allow so-called hyperchaotic systems (systems with more
than one positive Lyapunov exponent) to be synchronized.
Several proposals for "secure" communication schemes have
been advanced; major ones are reviewed and their strengths
and weaknesses are touched upon. Arrays of coupled chaotic
systems have received a great deal of attention lately and
have spawned a host of interesting and, in some cases,
counterintuitive phenomena including bursting above
synchronization thresholds, destabilizing transitions as
coupling increases (short-wavelength bifurcations), and
riddled basins. In addition, a general mathematical
framework for analyzing the stability of arrays with
arbitrary coupling configurations is outlined. Finally, the
topic of generalized synchronization is discussed, along
with data analysis techniques that can be used to decide
whether two systems satisfy the mathematical requirements of
generalized synchronization. (c) 1997 American Institute of
Physics.},
Language = {ENG},
Doi = {10.1063/1.166278},
Key = {fds174115}
}
@article{fds132720,
Author = {G.A. Johnson},
Title = {G.A. Johnson, G. Cates, X.J. Chen, G.P. Cofer, B.
Driehuys, W. Happer, L.W. Hedlund, B. Saam, M. Shattuck,
J. Swartz. Dynamics of magnetization in hyperpolarized
gas MRI of the lung. Magnetic Resonance in Medicine
submitted (1996).},
Year = {1996},
Key = {fds132720}
}
@article{fds132738,
Author = {G.A. Johnson},
Title = {G.A. Johnson, H. Beneveniste, R.T. Engelhardt, H. Qiu, L. W.
Hedlund, Magnetic resonance microscopy in basic studies of
brain structure and function. New York Academy of
Sciences. In press (1996).},
Year = {1996},
Key = {fds132738}
}
@article{fds132721,
Author = {G.A. Johnson},
Title = {G.A. Johnson, L.W. Hedlund. Functional imaging of the
lung. Nature Medicine 2, 1192 (1996).},
Year = {1996},
Key = {fds132721}
}
@article{fds132739,
Author = {G.A. Johnson},
Title = {G.A. Johnson, R.D. Black, G.D. Cates, X.J. Chen, B.
Driehuys, W. Happer, L.W. Hedlund, H. Middleton, M.D.
Shattuck, J. Swartz. Polarization dynamics of
hyperpolarized 3He in lung imaging. in "Proc., SMR 4th
Annual Scientific Meeting. New York, NY, 1996," p.
19.},
Year = {1996},
Key = {fds132739}
}
@article{fds174137,
Author = {SK Lewis and JL Farmer and RC Burghardt and GR Newton and GA Johnson and DL
Adelson, FW Bazer and TE Spencer},
Title = {Galectin 15 (LGALS15): a gene uniquely expressed in the
uteri of sheep and goats that functions in trophoblast
attachment.},
Journal = {Biology of reproduction},
Volume = {77},
Number = {6},
Pages = {1027-36},
Year = {2007},
Month = {December},
ISSN = {0006-3363},
url = {http://dx.doi.org/10.1095/biolreprod.107.063594},
Keywords = {Amino Acid Sequence • Animals • Cattle •
Cells, Cultured • Embryo Implantation •
Endometrium • Female • Galectins • Gene
Expression • Goats • Molecular Sequence Data
• Pregnancy • RNA, Messenger • Rabbits •
Ruminants • Sheep • Swine • Trophoblasts
• genetics • genetics* • metabolism •
metabolism* • physiology • physiology*},
Abstract = {Galectins are a family of secreted animal lectins with
biological roles in cell adhesion and migration. In sheep,
galectin 15 (LGALS15) is expressed specifically in the
endometrial luminal (LE) and superficial glandular (sGE)
epithelia of the uterus in concert with blastocyst
elongation during the peri-implantation period. The present
study examined LGALS15 expression in the uterus of cattle,
goats, and pigs. Although the bovine genome contains an
LGALS15-like gene, expressed sequence tags encoding LGALS15
mRNA were found only for sheep, and full-length LGALS15
cDNAs were cloned only from endometrial total RNA isolated
from pregnant sheep and goats, but not pregnant cattle or
pigs. Ovine and caprine LGALS15 were highly homologous at
the mRNA (95%) and protein (91%) levels, and all contained a
conserved carbohydrate recognition domain and RGD
recognition sequence for integrin binding. Endometrial
LGALS15 mRNA levels increased after Day 11 of both the
estrous cycle and pregnancy, and were considerably increased
after Day 15 of pregnancy in goats. In situ hybridization
detected abundant LGALS15 mRNA in endometrial LE and sGE of
early pregnant goats, but not in cattle or pigs.
Immunoreactive LGALS15 protein was present in endometrial
epithelia and conceptus trophectoderm of goat uteri and
detected within intracellular crystal structures in
trophectoderm and LE. Recombinant ovine and caprine LGALS15
proteins elicited a dose-dependent increase in ovine
trophectoderm cell attachment in vitro that was comparable
to bovine fibronectin. These results support the hypothesis
that LGALS15 is uniquely expressed in Caprinae endometria
and functions as an attachment factor important for
peri-implantation blastocyst elongation.},
Language = {eng},
Doi = {10.1095/biolreprod.107.063594},
Key = {fds174137}
}
@booklet{Thompson82,
Author = {W. M. Thompson and W. C. Meyers and M. Shaw and M. Bates and G. A. Johnson and L. W. Hedlund},
Title = {Gallbladder density and iodine concentration in humans
during oral cholecystography - a comparison of iopanoic acid
and iopronic acid},
Journal = {Investigative Radiology},
Volume = {17},
Number = {6},
Pages = {621 -- 628},
Year = {1982},
Key = {Thompson82}
}
@booklet{Thompson80a,
Author = {THOMPSON, WM and AMBERG, JR and LOWTHER, DT and SHAW, M and BATES, M and JOHNSON, GA},
Title = {GALLBLADDER DENSITY AND IODINE CONCENTRATION IN HUMANS
DURING ORAL CHOLECYSTOGRAPHY - A COMPARISON OF IOPANOIC ACID
AND IOPRONIC ACID},
Journal = {GASTROINTESTINAL RADIOLOGY},
Volume = {5},
Number = {1},
Pages = {83-83},
Publisher = {SPRINGER VERLAG},
Year = {1980},
Month = {January},
ISSN = {0364-2356},
url = {http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:A1980JN17700030&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=47d3190e77e5a3a53558812f597b0b92},
Key = {Thompson80a}
}
@article{fds132888,
Author = {WM Thompson and WC Meyers and M Shaw and M Bates and GA Johnson and LW
Hedlund},
Title = {Gallbladder density and iodine concentration in humans
during oral cholecystography. A comparison of iopanoic acid
and iopronic acid.},
Journal = {Investigative radiology, UNITED STATES},
Volume = {17},
Number = {6},
Pages = {621-8},
ISSN = {0020-9996},
Keywords = {Administration, Oral • Bile Acids and Salts •
Cholecystography* • Clinical Trials • Contrast
Media • Double-Blind Method • Gallbladder •
Humans • Iodine • Iodobenzenes • Iopanoic
Acid • administration & dosage* •
analysis},
Abstract = {A comparison of two oral cholecystopaques, iopanoic acid
(Telepaque) and iopronic acid (Oravue), was performed using
normal volunteers. Using a double-blind crossover design,
comparisons were made between the degree of gallbladder
opacification and the amount of iodine recovered from the
gallbladder. Bile was collected via a double lumen
intestinal tube before, during, and after stimulating
gallbladder contraction. There were no differences between
the two agents in terms of opacification or iodine
concentration. Only 19% of the administered dose of either
agent was recovered, and the maximum iodine concentration in
bile was 10 mg I/ml. The results suggest that this technique
has merit for future comparative studies of agents
concentrated in the gallbladder.},
Key = {fds132888}
}
@article{fds132816,
Author = {GA Johnson and JD Godwin and EK Fram},
Title = {Gated multiplanar cardiac computed tomography.},
Journal = {Radiology, UNITED STATES},
Volume = {145},
Number = {1},
Pages = {195-7},
Year = {1982},
Month = {October},
ISSN = {0033-8419},
Keywords = {Animals • Computers • Diatrizoate • Dogs
• Electrocardiography* • Heart • Tomography,
X-Ray Computed • diagnostic use • methods* •
radiography*},
Abstract = {Multiplanar reformatting was combined with
electrocardiographically gated computed tomography (CT) to
provide a three-dimensional assessment of the beating heart
of a live dog. Separate systolic and diastolic images were
made in transverse, sagittal, coronal, and paraxial views.
Three-dimensional contour images that outlined the cardiac
chambers and the myocardium were then made using a separate
research computer. This three-dimensional appreciation of
cardiac morphology could be extended to assess regional
function. Practical problems that limit the application of
these methods are discussed, along with the proposed
solutions.},
Key = {fds132816}
}
@booklet{Johnson82c,
Author = {Johnson, GA and Godwin, JD and Fram, EK},
Title = {Gated multiplanar cardiac computed tomography.},
Journal = {Radiology},
Volume = {145},
Number = {1},
Pages = {195-197},
Year = {1982},
Month = {October},
ISSN = {0033-8419},
url = {http://www.ncbi.nlm.nih.gov/pubmed/7122877},
Abstract = {Multiplanar reformatting was combined with
electrocardiographically gated computed tomography (CT) to
provide a three-dimensional assessment of the beating heart
of a live dog. Separate systolic and diastolic images were
made in transverse, sagittal, coronal, and paraxial views.
Three-dimensional contour images that outlined the cardiac
chambers and the myocardium were then made using a separate
research computer. This three-dimensional appreciation of
cardiac morphology could be extended to assess regional
function. Practical problems that limit the application of
these methods are discussed, along with the proposed
solutions.},
Doi = {10.1148/radiology.145.1.7122877},
Key = {Johnson82c}
}
@booklet{Wang99,
Author = {G. Y. Wang and G. A. Johnson and T. E. Spencer and F. W.
Bazer},
Title = {Generation of immortalized porcine uterine cell
lines.},
Journal = {Biology Of Reproduction},
Volume = {60},
Pages = {260 -- 261},
Year = {1999},
Key = {Wang99}
}
@article{fds268844,
Author = {Badea, A and Johnson, GA and Williams, RW},
Title = {Genetic dissection of the mouse brain using high-field
magnetic resonance microscopy.},
Journal = {Neuroimage},
Volume = {45},
Number = {4},
Pages = {1067-1079},
Year = {2009},
Month = {May},
ISSN = {1095-9572},
url = {http://www.ncbi.nlm.nih.gov/pubmed/19349225},
Keywords = {Animals • Brain • Magnetic Resonance Imaging
• Mice • Mice, Inbred C57BL • Mice, Inbred
DBA • Mice, Inbred Strains • Microscopy •
Species Specificity • anatomy & histology •
cytology* • genetics* • methods* •
physiology*},
Abstract = {Magnetic resonance (MR) imaging has demonstrated that
variation in brain structure is associated with differences
in behavior and disease state. However, it has rarely been
practical to prospectively test causal models that link
anatomical and functional differences in humans. In the
present study we have combined classical mouse genetics with
high-field MR to systematically explore and test such
structure-functional relations across multiple brain
regions. We segmented 33 regions in two parental
strains-C57BL/6J (B) and DBA/2J (D)-and in nine BXD
recombinant inbred strains. All strains have been studied
extensively for more than 20 years using a battery of
genetic, functional, anatomical, and behavioral assays. We
compared levels of variation within and between strains and
sexes, by region, and by system. Average within-strain
variation had a coefficient of variation (CV) of 1.6% for
the whole brain; while the CV ranged from 2.3 to 3.6% for
olfactory bulbs, cortex and cerebellum, and up to
approximately 18% for septum and laterodorsal thalamic
nucleus. Variation among strain averages ranged from 6.7%
for cerebellum, 7.6% for whole brain, 9.0% for cortex, up to
approximately 26% for the ventricles, laterodorsal thalamic
nucleus, and the interpeduncular nucleus. Heritabilities
averaged 0.60+/-0.18. Sex differences were not significant
with the possible (and unexpected) exception of the pons (
approximately 20% larger in males). A correlation matrix of
regional volumes revealed high correlations among
functionally related parts of the CNS (e.g., components of
the limbic system), and several high correlations between
regions that are not anatomically connected, but that may
nonetheless be functionally or genetically
coupled.},
Doi = {10.1016/j.neuroimage.2009.01.021},
Key = {fds268844}
}
@article{fds268843,
Author = {Badea, A and Johnson, GA and Williams, RW},
Title = {Genetic dissection of the mouse CNS using magnetic resonance
microscopy.},
Journal = {Curr Opin Neurol},
Volume = {22},
Number = {4},
Pages = {379-386},
Year = {2009},
Month = {August},
ISSN = {1473-6551},
url = {http://www.ncbi.nlm.nih.gov/pubmed/19542887},
Abstract = {PURPOSE OF REVIEW: Advances in magnetic resonance microscopy
(MRM) make it practical to map gene variants responsible for
structural variation in brains of many species, including
mice and humans. We review results of a systematic genetic
analysis of MRM data using as a case study a family of well
characterized lines of mice. RECENT ADVANCES: MRM has
matured to the point that we can generate high contrast,
high-resolution images even for species as small as a mouse,
with a brain merely 1/3000th the size of humans. We
generated 21.5-micron data sets for a diverse panel of BXD
mouse strains to gauge the extent of genetic variation, and
as a prelude to comprehensive genetic and genomic analyses.
Here we review MRM capabilities and image segmentation
methods; heritability of brain variation; covariation of the
sizes of brain regions; and correlations between MRM and
classical histological data sets. SUMMARY: The combination
of high throughput MRM and genomics will improve our
understanding of the genetic basis of structure-function
correlations. Sophisticated mouse models will be critical in
converting correlations into mechanisms and in determining
genetic and epigenetic causes of differences in disease
susceptibility.},
Doi = {10.1097/WCO.0b013e32832d9b86},
Key = {fds268843}
}
@article{fds268857,
Author = {Johnston, SM and Johnson, GA and Badea, CT},
Title = {Geometric calibration for a dual tube/detector micro-CT
system.},
Journal = {Med Phys},
Volume = {35},
Number = {5},
Pages = {1820-1829},
Year = {2008},
Month = {May},
ISSN = {0094-2405},
url = {http://www.ncbi.nlm.nih.gov/pubmed/18561657},
Keywords = {Algorithms • Animals • Artifacts •
Calibration • Equipment Design • Humans •
Metals • Mice • Models, Statistical • Models,
Theoretical • Phantoms, Imaging • Radiographic
Image Interpretation, Computer-Assisted •
Reproducibility of Results • Tomography Scanners, X-Ray
Computed • Tomography, X-Ray Computed • X-Rays
• instrumentation* • methods •
methods*},
Abstract = {The authors describe a dual tube/detector micro-computed
tomography (micro-CT) system that has the potential to
improve temporal resolution and material contrast in small
animal imaging studies. To realize this potential, it is
necessary to precisely calibrate the geometry of a dual
micro-CT system to allow the combination of projection data
acquired with each individual tube/detector in a single
reconstructed image. The authors present a geometric
calibration technique that uses multiple projection images
acquired with the two imaging chains while rotating a
phantom containing a vertical array of regularly spaced
metallic beads. The individual geometries of the imaging
chains are estimated from the phantom projection images
using analytical methods followed by a refinement procedure
based on nonlinear optimization. The geometric parameters
are used to create the cone beam projection matrices
required by the reconstruction process for each imaging
chain. Next, a transformation between the two projection
matrices is found that allows the combination of projection
data in a single reconstructed image. The authors describe
this technique, test it with a series of computer
simulations, and then apply it to data collected from their
dual tube/detector micro-CT system. The results demonstrate
that the proposed technique is accurate, robust, and
produces images free of misalignment artifacts.},
Doi = {10.1118/1.2900000},
Key = {fds268857}
}
@booklet{Middleditch89,
Author = {B. S. Middleditch and G. A. Johnson and R. R. Gregory and M.
A. Alejandro and B. M. Markaverich},
Title = {Gingerol analysis without artifact formation},
Journal = {Hrc-journal Of High Resolution Chromatography},
Volume = {12},
Number = {10},
Pages = {677 -- 679},
Year = {1989},
Month = {October},
Key = {Middleditch89}
}
@article{fds359545,
Author = {Nair, KS and Srivastava, C and Brown, RV and Koli, S and Choquet, H and Kang, HS and Kuo, Y-M and Grimm, SA and Sutherland, C and Badea, A and Johnson, GA and Zhao, Y and Yin, J and Okamoto, K and Clark, G and Borrás,
T and Zode, G and Kizhatil, K and Chakrabarti, S and John, SWM and Jorgenson, E and Jetten, AM},
Title = {GLIS1 regulates trabecular meshwork function and intraocular
pressure and is associated with glaucoma in
humans.},
Journal = {Nat Commun},
Volume = {12},
Number = {1},
Pages = {4877},
Year = {2021},
Month = {August},
url = {http://dx.doi.org/10.1038/s41467-021-25181-7},
Abstract = {Chronically elevated intraocular pressure (IOP) is the major
risk factor of primary open-angle glaucoma, a leading cause
of blindness. Dysfunction of the trabecular meshwork (TM),
which controls the outflow of aqueous humor (AqH) from the
anterior chamber, is the major cause of elevated IOP. Here,
we demonstrate that mice deficient in the Krüppel-like zinc
finger transcriptional factor GLI-similar-1 (GLIS1) develop
chronically elevated IOP. Magnetic resonance imaging and
histopathological analysis reveal that deficiency in GLIS1
expression induces progressive degeneration of the TM,
leading to inefficient AqH drainage from the anterior
chamber and elevated IOP. Transcriptome and cistrome
analyses identified several glaucoma- and extracellular
matrix-associated genes as direct transcriptional targets of
GLIS1. We also identified a significant association between
GLIS1 variant rs941125 and glaucoma in humans (P = 4.73 ×
10-6), further supporting a role for GLIS1 into glaucoma
etiology. Our study identifies GLIS1 as a critical regulator
of TM function and maintenance, AqH dynamics, and
IOP.},
Doi = {10.1038/s41467-021-25181-7},
Key = {fds359545}
}
@article{fds174286,
Author = {JT Self and TE Spencer and GA Johnson and J Hu and FW Bazer and G
Wu},
Title = {Glutamine synthesis in the developing porcine
placenta.},
Journal = {Biology of reproduction},
Volume = {70},
Number = {5},
Pages = {1444-51},
Year = {2004},
Month = {May},
ISSN = {0006-3363},
url = {http://dx.doi.org/10.1095/biolreprod.103.025486},
Keywords = {3-Methyl-2-Oxobutanoate Dehydrogenase (Lipoamide) •
Alanine • Alanine Transaminase • Amino Acids
• Amino Acids, Branched-Chain • Animals •
Biological Transport • Female • Glutamate-Ammonia
Ligase • Glutaminase • Glutamine • Placenta
• Pregnancy • Swine • Transaminases •
biosynthesis • biosynthesis* • enzymology •
growth & development* • metabolism •
metabolism*},
Abstract = {Glutamine plays a vital role in fetal carbon and nitrogen
metabolism and exhibits the highest fetal:maternal plasma
ratio among all amino acids in pigs. Such disparate
glutamine levels between mother and fetus suggest that
glutamine may be actively synthesized and released into the
fetal circulation by the porcine placenta. We hypothesized
that branched-chain amino acid (BCAA) metabolism in the
placenta plays an important role in placental glutamine
synthesis. This hypothesis was tested by studying
conceptuses from gilts on Days 20, 30, 35, 40, 45, 50, 60,
90, or 110 of gestation (n = 6 per day). Placental tissue
was analyzed for amino acid concentrations, BCAA transport,
BCAA degradation, and glutamine synthesis as well as the
activities of related enzymes (including BCAA transaminase,
branched-chain alpha-ketoacid dehydrogenase, glutamine
synthetase, glutamate-pyruvate transaminase, and
glutaminase). On all days of gestation, rates of BCAA
transamination were much greater than rates of
branched-chain alpha-ketoacid decarboxylation. The glutamate
generated from BCAA transamination was primarily directed to
glutamine synthesis and, to a much lesser extent, alanine
production. Placental BCAA transport, BCAA transamination,
glutamine synthesis, and activities of related enzymes
increased markedly between Days 20 and 40 of gestation, as
did glutamine in fetal allantoic fluid. Accordingly,
placental BCAA levels decreased after Day 20 of gestation in
association with a marked increase in BCAA catabolism and
concentrations of glutamine. There was no detectable
catabolism of glutamine in pig placenta throughout
pregnancy, which would ensure maximum output of glutamine by
this tissue. These novel results demonstrate glutamine
synthesis from BCAAs in pig placentae, aid in explaining the
abundance of glutamine in the fetus, and provide valuable
insight into the dynamic role of the placenta in fetal
metabolism and nutrition.},
Language = {eng},
Doi = {10.1095/biolreprod.103.025486},
Key = {fds174286}
}
@booklet{Self04,
Author = {J. T. Self and T. E. Spencer and G. A. Johnson and J. B. Hu and F. W. Bazer and G. Y. Wu},
Title = {Glutamine synthesis in the developing porcine
placental},
Journal = {Biology Of Reproduction},
Volume = {70},
Number = {5},
Pages = {1444 -- 1451},
Year = {2004},
Month = {May},
Key = {Self04}
}
@article{fds174092,
Author = {JJ Muniz and MM Joyce and JD Taylor 2nd, JR Burghardt and RC
Burghardt, GA Johnson},
Title = {Glycosylation dependent cell adhesion molecule 1-like
protein and L-selectin expression in sheep interplacentomal
and placentomal endometrium.},
Journal = {Reproduction (Cambridge, England)},
Volume = {131},
Number = {4},
Pages = {751-61},
Year = {2006},
Month = {April},
ISSN = {1470-1626},
url = {http://dx.doi.org/10.1530/rep.1.00855},
Keywords = {Actins • Animals • Blotting, Western •
Endometrium • Female • Fluorescent Antibody
Technique • Keratins • L-Selectin • Mucins
• Placenta • Pregnancy • Pregnancy, Animal
• Sheep • analysis • analysis* •
chemistry* • metabolism* • methods},
Abstract = {Glycosylation dependent cell adhesion molecule 1 (GlyCAM-1),
a mucin component of sheep histotroph produced by glandular
epithelium (GE) during early pregnancy, is hypothesized to
function in implantation. However, GlyCAM-1 is present in
uterine tissues subsequent to implantation suggesting
additional functions of this l-selectin-binding ligand. This
study focused on uterine GlyCAM-1 expression during
placentome development in sheep. Western blot analysis of
day 50 pregnant sheep identified 45, 40, and 25 kDa bands in
interplacentomal endometrium, 40 and 25 kDa bands in
placentomes, and 80 and 40 kDa bands in chorioallantois. The
GlyCAM-1 proteins in interplacentomal regions were
comparable to those detected in day 15-19 pregnant sheep,
however, the 80 kDa form was unique to chorioallantois, and
the absence of the 45 kDa GlyCAM-1 in placentomes indicated
differences between interplacentomal and placentomal
endometrium. Immunofluorescence identified GlyCAM-1 in
lumenal epithelium (LE), stromal fibroblasts, and vascular
smooth muscle cells. To better define its cellular
distribution, GlyCAM-1 was co-localized with either
epithelium-specific cytokeratin, smooth muscle-specific
alpha-smooth muscle actin (alpha SMA), or stromal-specific
vimentin. In interplacentomal endometrium, GlyCAM-1
co-localized with cytokeratin in LE but not in GE. GlyCAM-1
did not co-localize with alpha SMA, and was localized in the
extracellular matrix of vimentin-positive stroma. In
placentomes, GlyCAM-1 did not co-localize with cytokeratin,
but did co-localize with alpha SMA and vimentin. Thus, in
contrast to interplacentomal regions, GlyCAM-1 in
placentomes was predominantly localized in vasculature
rather than epithelial cells. Further, leukocytes expressing
L-selectin were localized to the endothelial surface of
GlyCAM-1-expressing vessels within placentomes. These data
suggest that GlyCAM-1 assumes distinct functions in
compartment-specific regions of the sheep
uterus.},
Language = {eng},
Doi = {10.1530/rep.1.00855},
Key = {fds174092}
}
@booklet{Muniz04,
Author = {J. J. Muniz and M. M. Joyce and R. C. Burghardt and G. A.
Johnson},
Title = {Glycosylation-dependent cell adhesion molecule 1 (GlyCAM-1)
expression in ovine placentomes implies roles in
hematotrophic support for conceptus development.},
Journal = {Biology Of Reproduction},
Pages = {212 -- 212},
Year = {2004},
Key = {Muniz04}
}
@article{fds268722,
Author = {Johnston, SM and Johnson, GA and Badea, CT},
Title = {GPU-based iterative reconstruction with total variation
minimization for micro-CT},
Journal = {Progress in Biomedical Optics and Imaging - Proceedings of
SPIE},
Volume = {7622},
Number = {PART 2},
Year = {2010},
ISBN = {9780819480231},
ISSN = {1605-7422},
url = {http://dx.doi.org/10.1117/12.844368},
Abstract = {Dynamic imaging with micro-CT often produces
poorly-distributed sets of projections, and reconstructions
of this data with filtered backprojection algorithms (FBP)
may be affected by artifacts. Iterative reconstruction
algorithms and total variation (TV) denoising are promising
alternatives to FBP, but may require running times that are
frustratingly long. This obstacle can be overcome by
implementing reconstruction algorithms on graphics
processing units (GPU). This paper presents an
implementation of a family of iterative reconstruction
algorithms with TV denoising on a GPU, and a series of tests
to optimize and compare the ability of different algorithms
to reduce artifacts. The mathematical and computational
details of the implementation are explored. The performance,
measured by the accuracy of the reconstruction versus the
running time, is assessed in simulations with a virtual
phantom and in an in vivo scan of a mouse. We conclude that
the simultaneous algebraic reconstruction technique with TV
minimization (SART-TV) is a time-effective reconstruction
algorithm for producing reconstructions with fewer artifacts
than FBP. © 2010 SPIE.},
Doi = {10.1117/12.844368},
Key = {fds268722}
}
@booklet{Teixeira96,
Author = {M. G. Teixeira and K. J. Austin and D. J. Perry and V. D.
Dooley and G. A. Johnson and T. R. Hansen},
Title = {Granulocyte chemotactic protein-2 (GCP-2) is a
pregnancy-associated uterine chemokine.},
Journal = {Biology Of Reproduction},
Volume = {54},
Pages = {190 -- 190},
Year = {1996},
Key = {Teixeira96}
}
@booklet{Newton99,
Author = {G. R. Newton and S. Woldesenbet and T. Green and H. Powell and K. Sonnier and G. A. Johnson},
Title = {H antigen expression by ovine endometrial
cells.},
Journal = {Biology Of Reproduction},
Volume = {60},
Pages = {120 -- 120},
Year = {1999},
Key = {Newton99}
}
@article{fds132743,
Author = {G.A. Johnson},
Title = {H. Qiu, L.W. Hedlund, H. Benveniste, S.L. Gewalt, G.A.
Johnson. Evaluation of a glycine antagonist in rat focal
cerebral ischemia by diffusion-weighted MR microscopy.
in "Proc., SMR 4th Annual Scientific Meeting. New
York, NY, 1996," p. 505.},
Year = {1996},
Key = {fds132743}
}
@article{fds174164,
Author = {AR Diani and MJ Mulholland and KL Shull and MF Kubicek and GA Johnson and HJ Schostarez and MN Brunden and AE Buhl},
Title = {Hair growth effects of oral administration of finasteride, a
steroid 5 alpha-reductase inhibitor, alone and in
combination with topical minoxidil in the balding stumptail
macaque.},
Journal = {The Journal of clinical endocrinology and
metabolism},
Volume = {74},
Number = {2},
Pages = {345-50},
Year = {1992},
Month = {February},
ISSN = {0021-972X},
Keywords = {Administration, Oral • Administration, Topical •
Androstenes • Animals • Azasteroids •
Chromatography, High Pressure Liquid •
Dihydrotestosterone • Drug Interactions •
Finasteride • Hair • Macaca • Male •
Minoxidil • Reference Values • Testosterone •
Testosterone 5-alpha-Reductase • administration &
dosage • antagonists & inhibitors* • blood •
drug effects* • pharmacology* • physiology •
urine},
Abstract = {A 5 alpha-reductase inhibitor, finasteride, was administered
orally at 0.5 mg/day, alone or in combination with topical
2% minoxidil, for 20 weeks to determine the effects on scalp
hair growth in balding adult male stumptail macaque monkeys.
A 7-day dose-finding study showed that both 0.5- and 2.0-mg
doses of the drug produced a similar diminution in serum
dihydrotestosterone (DHT) in male stumptails. Hair growth
was evaluated by shaving and weighing scalp hair at baseline
and at 4-week intervals during treatment to obtain
cumulative delta hair weight (sum of the 4-week changes in
hair weight from baseline) for the 20-week study. The
activity of the 5 alpha-reductase enzyme was assessed by RIA
of serum testosterone (T) and DHT at 4-week intervals. The
combination of finasteride and minoxidil generated
significant augmentation of hair weight (additive effect)
compared to either drug alone. Finasteride increased hair
weight in four of five monkeys. When the data of the one
nonresponsive monkey were excluded, finasteride elicited a
significant elevation in hair weight compared to topical
vehicle alone. Minoxidil also evoked a significant increase
in hair weight compared to vehicle alone. Serum T was
unchanged, whereas serum DHT was significantly depressed in
monkeys that received either finasteride or the combination
of finasteride and minoxidil. These data suggest that
inhibition of the conversion of T to DHT by this 5
alpha-reductase inhibitor reverses the balding process and
enhances hair regrowth by topical minoxidil in the male
balding stumptail macaque.},
Language = {eng},
Key = {fds174164}
}
@booklet{Diani92,
Author = {A. R. Diani and M. J. Mulholland and K. L. Shull and M. F.
Kubicek and G. A. Johnson and H. J. Schostarez and M. N.
Brunden and A. E. Buhl},
Title = {Hair-growth effects of oral-administration of finasteride, a
steroid 5-alpha-reductase inhibitor, alone and in
combination with topical minoxidil in the balding stumptail
macaque},
Journal = {Journal Of Clinical Endocrinology And Metabolism},
Volume = {74},
Number = {2},
Pages = {345 -- 350},
Year = {1992},
Month = {February},
Key = {Diani92}
}
@article{fds268693,
Author = {Johnston, SM and Johnson, GA and Badea, CT},
Title = {Helical dual source cone-beam micro-CT},
Journal = {2014 IEEE 11th International Symposium on Biomedical
Imaging, ISBI 2014},
Pages = {177-180},
Year = {2014},
Month = {July},
ISBN = {9781467319591},
url = {http://dx.doi.org/10.1109/isbi.2014.6867838},
Abstract = {While helical scanning is well established in the clinical
arena, most micro-CT scanners use circular cone beam
trajectories and approximate reconstructions based on a
filtered backprojection (FBP) algorithm. This may be
sufficient for some applications, but in studies of larger
animals, such as rats, the size of the detector can
constrain the field of view and extend scan time. To address
this problem, we have designed and implemented helical
scanning and reconstruction procedures for an
in-house-developed dual source cone-beam micro-CT system.
The reconstruction uses a simultaneous algebraic
reconstruction technique combined with total variation
regularization (SART-TV). We implemented this algorithm on a
graphics processing unit (GPU) to reduce run time. The
results demonstrate the speed and accuracy of the GPU-based
SART-TV algorithm. The helical scan enables the
reconstruction of volumes with extended field of view for
whole body micro-CT imaging of large rodents.},
Doi = {10.1109/isbi.2014.6867838},
Key = {fds268693}
}
@article{fds174225,
Author = {DG Johnston and GA Johnson and KG Alberti and GH Millward-Sadler and J
Mitchell, R Wright},
Title = {Hepatic regeneration and metabolism after partial
hepatectomy in diabetic rats: effects of insulin
therapy.},
Journal = {European journal of clinical investigation},
Volume = {16},
Number = {5},
Pages = {384-90},
Year = {1986},
Month = {October},
ISSN = {0014-2972},
Keywords = {Adenine Nucleotides • Animals • DNA •
Diabetes Mellitus, Experimental • Glucose •
Hepatectomy* • Insulin • Lactates • Lactic
Acid • Liver • Liver Glycogen • Liver
Regeneration • Male • Organ Size • Pyruvates
• Pyruvic Acid • Rats • Rats, Inbred Strains
• Water • analysis • biosynthesis •
blood • drug effects* • drug therapy •
metabolism • metabolism* • therapeutic
use*},
Abstract = {The effect of insulin deficiency on liver regeneration has
been assessed in untreated v. insulin-treated streptozotocin
(65 mg kg-1) diabetic rats, 12, 24 and 48 h after partial
hepatectomy. Dry weight of regenerating liver increased from
12 to 48 h after partial hepatectomy and insulin treatment
caused a further minor increase at 24 h. [6-3H]Thymidine
uptake in untreated rats peaked at 24 h (12.5 +/- 3.4% of
total cells labelled). Insulin therapy produced a delayed
168% rise in uptake at 48 h. Insulin deficiency alone in
sham-operated animals caused a 33% decrease in hepatic
[ATP], while [ADP] rose by 43% and [AMP] by 86% at 12 h.
Partial hepatectomy produced only minor further
abnormalities in untreated animals. Insulin therapy
increased hepatic [ATP] and decreased [ADP] and [AMP] 12 h
after partial hepatectomy, but [ATP] remained decreased
(15%) and [ADP] and [AMP] increased (45% and 73%
respectively) compared with insulin-treated sham-operated
controls. Metabolite changes observed after partial
hepatectomy in untreated animals, including a decrease in
hepatic [glycogen] and increases in [triglyceride] and the
ratios of [lactate]:[pyruvate] and [3-hydroxybutyrate]:[acetoacetate],
were partially reversed by insulin treatment. Insulin
deficiency thus impairs regeneration after partial
hepatectomy and magnifies the decline in hepatic
intracellular energy state and the metabolite changes
associated with liver regrowth.},
Language = {eng},
Key = {fds174225}
}
@article{fds161595,
Author = {DG Johnston and GA Johnson and KG Alberti and GH Millward-Sadler and J
Mitchell, R Wright},
Title = {Hepatic regeneration and metabolism after partial
hepatectomy in normal rats: effects of insulin
therapy.},
Journal = {European journal of clinical investigation,
ENGLAND},
Volume = {16},
Number = {5},
Pages = {376-83},
Year = {1986},
Month = {October},
ISSN = {0014-2972},
Keywords = {Adenine Nucleotides • Animals • DNA • Glucose
• Glycogen • Hepatectomy* • Insulin •
Liver • Liver Regeneration • Male • Organ
Size • Rats • Rats, Inbred Strains •
Thymidine • Water • analysis • biosynthesis
• cytology • drug effects* • metabolism
• metabolism* • therapeutic use*},
Abstract = {The effect of insulin therapy on liver regeneration has been
studied in normal fed rats 12, 24 and 48 h after partial
hepatectomy. Dry weight of regenerating liver increased
between 12 and 48 h after partial hepatectomy and was
unaffected by insulin therapy. [6-3H] Thymidine uptake
peaked at 24-h (24.7 +/- 2.4% of total liver cells) and
insulin treatment had no additional effect. At 12-h after
partial hepatectomy, hepatic [ATP] was decreased 15%, while
[ADP] and [AMP] were increased 47% and 83% respectively
compared with sham-operated animals. Partial hepatectomy
also caused an increase in hepatic [triglyceride], a
decrease in hepatic [glycogen] and an increase in the levels
of glucose and several glycolytic intermediates. The hepatic
redox ratios, [lactate]:[pyruvate] and [3-hydroxybutyrate]:[acetoacetate],
were elevated. Insulin therapy had only minor effects on
hepatic adenine nucleotide levels, intermediary metabolite
concentrations or intrahepatic redox ratios after partial
hepatectomy. These findings suggest a decreased hepatic
intracellular energy state in regenerating liver; insulin
therapy in normal rats does not influence this metabolic
change nor the regenerative response.},
Key = {fds161595}
}
@booklet{Johnston86a,
Author = {D. G. Johnston and G. A. Johnson and K. G. M. M. Alberti and G. H. Millwardsadler and J. Mitchell and R.
Wright},
Title = {Hepatic regeneration and metabolism after
partial-hepatectomy in diabetic rats - effects of insulin
therapy},
Journal = {European Journal Of Clinical Investigation},
Volume = {16},
Number = {5},
Pages = {384 -- 390},
Year = {1986},
Month = {October},
Key = {Johnston86a}
}
@booklet{Johnston86,
Author = {D. G. Johnston and G. A. Johnson and K. G. M. M. Alberti and G. H. Millwardsadler and J. Mitchell and R.
Wright},
Title = {Hepatic regeneration and metabolism after
partial-hepatectomy in normal rats - effects of insulin
therapy},
Journal = {European Journal Of Clinical Investigation},
Volume = {16},
Number = {5},
Pages = {376 -- 383},
Year = {1986},
Month = {October},
Key = {Johnston86}
}
@article{fds174120,
Author = {J Shen-Gunther and JL Walker and GA Johnson and RS
Mannel},
Title = {Hepatic venoocclusive disease as a complication of whole
abdominopelvic irradiation and treatment with the
transjuglar intrahepatic portosystemic shunt: case report
and literature review.},
Journal = {Gynecologic oncology},
Volume = {61},
Number = {2},
Pages = {282-6},
Year = {1996},
Month = {May},
ISSN = {0090-8258},
Keywords = {Abdomen • Adenocarcinoma • Ascites •
Endometrial Neoplasms • Female • Hepatic
Veno-Occlusive Disease • Humans • Jugular Veins
• Middle Aged • Pelvis • Portasystemic Shunt,
Surgical* • Radiotherapy • Randomized Controlled
Trials as Topic • Stents • adverse effects* •
complications • etiology* • instrumentation •
radiotherapy • surgery},
Abstract = {We report the novel use of the transjugular intrahepatic
portosystemic shunt (TIPS) procedure for the treatment of
intractable ascites due to hepatic venooclusive disease as a
result of whole abdominopelvic radiotherapy. A patient with
Stage III endometrioid carcinoma of the endometrium treated
with postoperative whole abdominopelvic irradiation
developed intractable ascites. Multiple paracenteses and
computerized tomography were negative for recurrent
carcinoma. Liver biopsy demonstrated hepatic venoocclusive
disease, a rare complication of therapeutic radiation
involving the liver. Successful relief of ascites and its
adverse symptomology were achieved with the transjugular
intrahepatic portosystemic shunt. Relevant literature
regarding the pathogenesis, prognosis, and treatment of
radiotherapy-related hepatic venoocclusive disease are
reviewed.},
Language = {eng},
Key = {fds174120}
}
@booklet{Shengunther96,
Author = {J. Shengunther and J. L. Walker and G. A. Johnson and R. S.
Mannel},
Title = {Hepatic venoocclusive disease as a complication of whole
abdominopelvic irradiation and treatment with the
transjugular intrahepatic portosystemic shunt: Case report
and literature review},
Journal = {Gynecologic Oncology},
Volume = {61},
Number = {2},
Pages = {282 -- 286},
Year = {1996},
Month = {May},
Key = {Shengunther96}
}
@article{fds371720,
Author = {Johnston, DG and Johnson, GA and Alberti, KG},
Title = {Hepatotrophic factors: implications for diabetes
mellitus.},
Journal = {Ciba Found Symp},
Number = {55},
Pages = {357-373},
Year = {1977},
ISSN = {0300-5208},
url = {http://dx.doi.org/10.1002/9780470720363.ch18},
Keywords = {Adenine Nucleotides • Animals • Diabetes
Complications • Diabetes Mellitus • Diabetes
Mellitus, Experimental • Dogs • Fatty Liver •
Glucose • Humans • Injections, Subcutaneous •
Insulin • Liver • Liver Cirrhosis • Liver
Glycogen • Liver Regeneration • Portal Vein •
Rats • administration & dosage* • analysis •
drug therapy* • etiology • metabolism},
Abstract = {In view of the importance of insulin in hepatic cell
proliferation and regeneration, disturbances might be
expected in these processes in diabetics. The relative
importnace of insulin replacement given intraportally rather
than subcutaneously is discussed. Results are presented
showing that even when normoglycaemia is achieved with
peripheral insulin infusion using the 'artificial pancreas'
there are still abnormalities in intermediary metabolism.
The incidence of cirrhosis in diabetes is reviewed and it is
concluded that the evidence is poor for an increase in
diabetics. Finally it is shown that in the normal diabetic
rat changes are observed after partial hepatectomy
consistent with an increase in redox potential within the
regenerating liver. Insulin treatment improves redox status
but does not completely reverse the changes
shown.},
Language = {eng},
Doi = {10.1002/9780470720363.ch18},
Key = {fds371720}
}
@booklet{Johnson99,
Author = {G. A. Johnson and N. M. Ghoniem},
Title = {Hierarchical modeling of C and Si nano-cluster nucleation
utilizing quantum and statistical mechanics},
Journal = {Journal Of Computer-aided Materials Design},
Volume = {6},
Number = {2-3},
Pages = {337 -- 347},
Year = {1999},
Key = {Johnson99}
}
@booklet{Thompson83a,
Author = {W. M. Thompson and R. A. Halvorsen and R. K. Gedgaudas and F. M. Kelvin and R. P. Rice and S. Woodfield and G. A.
Johnson and L. W. Hedlund and D. B. Jorgensen},
Title = {High kvp vs low kvp for t-tube and operative
cholangiography},
Journal = {Radiology},
Volume = {146},
Number = {3},
Pages = {635 -- 642},
Year = {1983},
Key = {Thompson83a}
}
@article{fds268910,
Author = {Thompson, WM and Halvorsen, RA and Gedgaudas, RK and Kelvin, FM and Rice, RP and Woodfield, S and Johnson, GA and Hedlund, LW and Jorgensen,
DB},
Title = {High kVp vs. low kVp for T-tube and operative
cholangiography.},
Journal = {Radiology},
Volume = {146},
Number = {3},
Pages = {635-642},
Year = {1983},
Month = {March},
ISSN = {0033-8419},
url = {http://www.ncbi.nlm.nih.gov/pubmed/6828675},
Keywords = {Cholangiography • Cholecystectomy • Cholelithiasis
• Diatrizoate • Diatrizoate Meglumine •
Humans • Intraoperative Care • Models, Structural
• Radiation Dosage • analogs & derivatives* •
diagnostic use* • methods* • radiography*},
Abstract = {Based on several considerations, high kVp and high contrast
agent concentration should produce better-quality operative
and T-tube cholangiograms than the currently recommended low
kVp and low contrast agent concentration. To test this
theory, two kinds of studies were performed. In a laboratory
phantom, the influence of kVp and contrast agent
concentration on detectability of different size phantom
stones was evaluated. High kVp and high contrast agent
concentration (110 kVp, 38% iodine) were also compared with
low kVp and low contrast agent concentration (75 kVp, 15%
iodine) in 62 patients undergoing operative or T-tube
cholangiography. Almost all phantom stones were well shown
with all kVps and iodine concentrations. As the kVp was
raised there was a mild decrease in stone detectability but
this decrease was partially corrected by raising the iodine
concentration. Overall stone detectability with high kVp and
high contrast agent concentration technique was better than
or similar to the currently recommended low kVp and low
contrast agent concentration technique. Evaluation of the
direct cholangiograms by five radiologists revealed that the
high kVp, high contrast agent concentration studies were
superior or similar to the low kVp and low contrast agent
concentration radiographs in 70% of the cases. Based on
these results high kVp (100-110) and a high contrast agent
concentration (38%) are recommended for direct
cholangiography.},
Doi = {10.1148/radiology.146.3.6828675},
Key = {fds268910}
}
@booklet{Silverman82b,
Author = {Silverman, PM and Johnson, GA and Korobkin, M and Thompson,
WM},
Title = {HIGH RESOLUTION MULTIPLANAR CT IMAGES OF THE
LARYNX},
Journal = {Investigative Radiology},
Volume = {17},
Number = {4},
Pages = {S34-S34},
Publisher = {Ovid Technologies (Wolters Kluwer Health)},
Year = {1982},
Month = {July},
ISSN = {0020-9996},
url = {http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:A1982PA96700153&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=47d3190e77e5a3a53558812f597b0b92},
Doi = {10.1097/00004424-198207000-00161},
Key = {Silverman82b}
}
@article{fds268808,
Author = {Liu, C and Li, W and Johnson, GA and Wu, B},
Title = {High-field (9.4 T) MRI of brain dysmyelination by
quantitative mapping of magnetic susceptibility.},
Journal = {Neuroimage},
Volume = {56},
Number = {3},
Pages = {930-938},
Year = {2011},
Month = {June},
ISSN = {1095-9572},
url = {http://www.ncbi.nlm.nih.gov/pubmed/21320606},
Keywords = {Algorithms • Animals • Anisotropy • Axons
• Brain • Brain Mapping • Demyelinating
Diseases • Diffusion Tensor Imaging • Echo-Planar
Imaging • Electromagnetic Fields* • Fourier
Analysis • Image Processing, Computer-Assisted •
Mice • Mice, Inbred C3H • Mice, Neurologic Mutants
• Myelin Sheath • Tissue Fixation • methods*
• pathology • pathology* •
physiology},
Abstract = {The multilayered myelin sheath wrapping around nerve axons
is essential for proper functioning of the central nervous
system. Abnormal myelination leads to a wide range of
neurological diseases and developmental disorders.
Non-invasive imaging of myelin content is of great clinical
importance. The present work demonstrated that loss of
myelin in the central nervous system of the shiverer mouse
results in a dramatic reduction of magnetic susceptibility
in white matter axons. The reduction resulted in a near
extinction of susceptibility contrast between gray and white
matter. Quantitative magnetic susceptibility imaging and
diffusion tensor imaging were conducted on a group of
control and shiverer mice at 9.4 T. We measured the
resonance frequency distribution of the whole brain for each
mouse. Magnetic susceptibility maps were computed and
compared between the two groups. It was shown that the
susceptibility contrast between gray and white matter was
reduced by 96% in the shiverer compared to the controls.
Diffusion measurements further confirmed intact fiber
pathways in the shiverer mice, ruling out the possibility of
axonal injury and its potential contribution to the altered
susceptibility. As an autosomal recessive mutation, shiverer
is characterized by an almost total lack of central nervous
system myelin. Our data provide new evidences indicating
that myelin is the predominant source of susceptibility
differences between deep gray and white matter observed in
magnetic resonance imaging. More importantly, the present
study suggests that quantitative magnetic susceptibility is
a potential endogenous biomarker for myelination.},
Language = {eng},
Doi = {10.1016/j.neuroimage.2011.02.024},
Key = {fds268808}
}
@article{fds132762,
Author = {X Zhou and GP Cofer and SA Suddarth and GA Johnson},
Title = {High-field MR microscopy using fast spin-echoes.},
Journal = {Magnetic resonance in medicine : official journal of the
Society of Magnetic Resonance in Medicine / Society of
Magnetic Resonance in Medicine, UNITED STATES},
Volume = {30},
Number = {1},
Pages = {60-7},
Year = {1993},
Month = {July},
ISSN = {0740-3194},
Keywords = {Animals • Brain • Brain Neoplasms • Cerebral
Cortex • Corpus Callosum • Humans • Image
Enhancement • Image Processing, Computer-Assisted
• Magnetic Resonance Imaging* • Melanoma •
Microscopy* • Models, Structural • Rats •
anatomy & histology • methods •
pathology},
Abstract = {Fast spin-echo imaging has been investigated with attention
to the requirements and opportunities for high-field MR
microscopy. Two- and three-dimensional versions were
implemented at 2.0 T, 7.1 T, and 9.4 T. At these fields, at
least eight echoes were collectable with a 10 ms TE from
fixed tissue specimens and living animals, giving an
eightfold improvement in imaging efficiency. To reduce the
phase-encoding gradient amplitude and its duty cycle, a
modified pulse sequence with phase accumulation was
developed. Images obtained using this pulse sequence
exhibited comparable signal-to-noise (SNR) to those obtained
from the conventional fast spin-echo pulse sequences. Signal
losses due to imperfections in RF pulses and lack of phase
rewinders were offset in this sequence by reduced diffusion
losses incurred with the gradients required for MR
microscopy. Image SNR, contrast, edge effects and spatial
resolution for three k-space sampling schemes were studied
experimentally and theoretically. One method of sampling
k-space, 4-GROUP FSE, was found particularly useful in
producing varied T2 contrast at high field. Two-dimensional
images of tissue specimens were obtained in a total
acquisition time of 1 to 2 min with in-plane resolution
between 30 to 70 microns, and 3D images with 256(3) arrays
were acquired from fixed rat brain tissue (isotropic voxel =
70 microns) and a living rat (isotropic voxel = 117 microns)
in approximately 4.5 h.},
Key = {fds132762}
}
@booklet{Zhou93a,
Author = {Zhou, X and Cofer, GP and Suddarth, SA and Johnson,
GA},
Title = {High-field MR microscopy using fast spin-echoes.},
Journal = {Magn Reson Med},
Volume = {30},
Number = {1},
Pages = {60-67},
Year = {1993},
Month = {July},
ISSN = {0740-3194},
url = {http://www.ncbi.nlm.nih.gov/pubmed/8371676},
Abstract = {Fast spin-echo imaging has been investigated with attention
to the requirements and opportunities for high-field MR
microscopy. Two- and three-dimensional versions were
implemented at 2.0 T, 7.1 T, and 9.4 T. At these fields, at
least eight echoes were collectable with a 10 ms TE from
fixed tissue specimens and living animals, giving an
eightfold improvement in imaging efficiency. To reduce the
phase-encoding gradient amplitude and its duty cycle, a
modified pulse sequence with phase accumulation was
developed. Images obtained using this pulse sequence
exhibited comparable signal-to-noise (SNR) to those obtained
from the conventional fast spin-echo pulse sequences. Signal
losses due to imperfections in RF pulses and lack of phase
rewinders were offset in this sequence by reduced diffusion
losses incurred with the gradients required for MR
microscopy. Image SNR, contrast, edge effects and spatial
resolution for three k-space sampling schemes were studied
experimentally and theoretically. One method of sampling
k-space, 4-GROUP FSE, was found particularly useful in
producing varied T2 contrast at high field. Two-dimensional
images of tissue specimens were obtained in a total
acquisition time of 1 to 2 min with in-plane resolution
between 30 to 70 microns, and 3D images with 256(3) arrays
were acquired from fixed rat brain tissue (isotropic voxel =
70 microns) and a living rat (isotropic voxel = 117 microns)
in approximately 4.5 h.},
Doi = {10.1002/mrm.1910300110},
Key = {Zhou93a}
}
@booklet{Drayer84,
Author = {DRAYER, BP and HERFKENS, RJ and JOHNSON, GA and HEINZ, ER and YEATES,
AE},
Title = {HIGH-FIELD NMR IMAGING (1.5T) IN A HOSPITAL
ENVIRONMENT},
Journal = {AMERICAN JOURNAL OF NEURORADIOLOGY},
Volume = {5},
Number = {5},
Pages = {669-669},
Year = {1984},
ISSN = {0195-6108},
url = {http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:A1984TG20900058&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=47d3190e77e5a3a53558812f597b0b92},
Key = {Drayer84}
}
@article{fds268875,
Author = {Nahrendorf, M and Badea, C and Hedlund, LW and Figueiredo, J-L and Sosnovik, DE and Johnson, GA and Weissleder, R},
Title = {High-resolution imaging of murine myocardial infarction with
delayed-enhancement cine micro-CT.},
Journal = {Am J Physiol Heart Circ Physiol},
Volume = {292},
Number = {6},
Pages = {H3172-H3178},
Year = {2007},
Month = {June},
ISSN = {0363-6135},
url = {http://www.ncbi.nlm.nih.gov/pubmed/17322414},
Keywords = {Animals • Cineradiography • Contrast Media •
Coronary Vessels • Disease Models, Animal •
Feasibility Studies • Female • Iopamidol •
Ligation • Mice • Mice, Inbred C57BL •
Myocardial Infarction • Radiographic Image
Interpretation, Computer-Assisted* • Reproducibility of
Results • Time Factors • Tomography, X-Ray
Computed* • Ventricular Function, Left •
Ventricular Remodeling • diagnostic use • methods*
• physiopathology • radiography* •
surgery},
Abstract = {The objective of this study was to determine the feasibility
of delayed-enhancement micro-computed tomography (microCT)
imaging to quantify myocardial infarct size in experimental
mouse models. A total of 20 mice were imaged 5 or 35 days
after surgical ligation of the left coronary artery or sham
surgery (n=6 or 7 per group). We utilized a prototype
microCT that covers a three-dimensional (3D) volume with an
isotropic spatial resolution of 100 microm. A series of
image acquisitions were started after a 200 microl bolus of
a high-molecular-weight blood pool CT agent to outline the
ventricles. CT imaging was continuously performed over 60
min, while an intravenous constant infusion with iopamidol
370 was started at a dosage of 1 ml/h. Thirty minutes after
the initiation of this infusion, signal intensity in
Hounsfield units was significantly higher in the infarct
than in the remote, uninjured myocardium. Cardiac morphology
and motion were visualized with excellent contrast and in
fine detail. In vivo CT determination of infarct size at the
midventricular level was in good agreement with ex vivo
staining with triphenyltetrazolium chloride [5 days
post-myocardial infarction (MI): r(2)=0.86, P<0.01; 35 days
post-MI: r(2)=0.92, P<0.01]. In addition, we detected
significant left ventricular remodeling consisting of left
ventricular dilation and decreased ejection fraction. 3D
cine microCT reliably and rapidly quantifies infarct size
and assesses murine anatomy and physiology after coronary
ligation, despite the small size and fast movement of the
mouse heart. This efficient imaging tool is a valuable
addition to the current phenotyping armamentarium and will
allow rapid testing of novel drugs and cell-based
interventions in murine models.},
Doi = {10.1152/ajpheart.01307.2006},
Key = {fds268875}
}
@article{fds268832,
Author = {Howles, GP and Ghaghada, KB and Qi, Y and Mukundan, S and Johnson,
GA},
Title = {High-resolution magnetic resonance angiography in the mouse
using a nanoparticle blood-pool contrast
agent.},
Journal = {Magn Reson Med},
Volume = {62},
Number = {6},
Pages = {1447-1456},
Year = {2009},
Month = {December},
ISSN = {1522-2594},
url = {http://www.ncbi.nlm.nih.gov/pubmed/19902507},
Keywords = {Animals • Cerebral Arteries • Contrast Media
• Gadolinium • Image Enhancement • Liposomes
• Magnetic Resonance Angiography • Male •
Mice • Mice, Inbred C57BL • Nanoparticles •
anatomy & histology* • chemistry • chemistry*
• diagnostic use* • methods*},
Abstract = {High-resolution magnetic resonance angiography is already a
useful tool for studying mouse models of human disease.
Magnetic resonance angiography in the mouse is typically
performed using time-of-flight contrast. In this work, a new
long-circulating blood-pool contrast agent-a liposomal
nanoparticle with surface-conjugated gadolinium (SC-Gd
liposomes)-was evaluated for use in mouse neurovascular
magnetic resonance angiography. A total of 12 mice were
imaged. Scan parameters were optimized for both
time-of-flight and SC-Gd contrast. Compared to
time-of-flight contrast, SC-Gd liposomes (0.08 mmol/kg)
enabled improved small-vessel contrast-to-noise ratio,
larger field of view, shorter scan time, and imaging of
venous structures. For a limited field of view,
time-of-flight and SC-Gd were not significantly different;
however, SC-Gd provided better contrast-to-noise ratio when
the field of view encompassed the whole brain (P < 0.001) or
the whole neurovascular axis (P < 0.001). SC-Gd allowed
acquisition of high-resolution magnetic resonance
angiography (52 x 52 x 100 micrometer(3) or 0.27 nL), with
123% higher (P < 0.001) contrast-to-noise ratio in
comparable scan time ( approximately 45 min). Alternatively,
SC-Gd liposomes could be used to acquire high-resolution
magnetic resonance angiography (0.27 nL) with 32% higher
contrast-to-noise ratio (P < 0.001) in 75% shorter scan time
(12 min).},
Language = {eng},
Doi = {10.1002/mrm.22154},
Key = {fds268832}
}
@article{fds268873,
Author = {Petiet, AE and Kaufman, MH and Goddeeris, MM and Brandenburg, J and Elmore, SA and Johnson, GA},
Title = {High-resolution magnetic resonance histology of the
embryonic and neonatal mouse: a 4D atlas and morphologic
database.},
Journal = {Proc Natl Acad Sci U S A},
Volume = {105},
Number = {34},
Pages = {12331-12336},
Year = {2008},
Month = {August},
ISSN = {1091-6490},
url = {http://www.ncbi.nlm.nih.gov/pubmed/18713865},
Keywords = {Anatomy, Cross-Sectional • Animals • Animals,
Newborn • Databases, Factual • Embryo, Mammalian
• Embryonic Development* • Heart Septal Defects
• Imaging, Three-Dimensional • Magnetic Resonance
Imaging • Mice • Mice, Mutant Strains • Mice,
Transgenic • Microscopy • Time Factors •
anatomy & histology* • instrumentation •
methods*},
Abstract = {Engineered mice play an ever-increasing role in defining
connections between genotype and phenotypic expression. The
potential of magnetic resonance microscopy (MRM) for
morphologic phenotyping in the mouse has previously been
demonstrated; however, applications have been limited by
long scan times, availability of the technology, and a
foundation of normative data. This article describes an
integrated environment for high-resolution study of normal,
transgenic, and mutant mouse models at embryonic and
neonatal stages. Three-dimensional images are shown at an
isotropic resolution of 19.5 microm (voxel volumes of 8 pL),
acquired in 3 h at embryonic days 10.5-19.5 (10 stages) and
postnatal days 0-32 (6 stages). A web-accessible atlas
encompassing this data was developed, and for critical
stages of embryonic development (prenatal days 14.5-18.5),
>200 anatomical structures have been identified and labeled.
Also, matching optical histology and analysis tools are
provided to compare multiple specimens at multiple
developmental stages. The utility of the approach is
demonstrated in characterizing cardiac septal defects in
conditional mutant embryos lacking the Smoothened receptor
gene. Finally, a collaborative paradigm is presented that
allows sharing of data across the scientific community. This
work makes magnetic resonance microscopy of the mouse embryo
and neonate broadly available with carefully annotated
normative data and an extensive environment for
collaborations.},
Doi = {10.1073/pnas.0805747105},
Key = {fds268873}
}
@booklet{Blinder85a,
Author = {BLINDER, RA and HERFKENS, RJ and COLEMAN, RE and JOHNSON, GA and SCHENCK, JF and HART, HR and FOSTER, TH and EDELSTEIN,
WA},
Title = {HIGH-RESOLUTION MRI IMAGING AT 1.5T USING SURFACE
COILS},
Journal = {JOURNAL OF NUCLEAR MEDICINE},
Volume = {26},
Number = {5},
Pages = {P8-P8},
Year = {1985},
ISSN = {0161-5505},
url = {http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:A1985AGS2100042&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=47d3190e77e5a3a53558812f597b0b92},
Key = {Blinder85a}
}
@booklet{Silverman82,
Author = {Silverman, PM and Johnson, GA and Korobkin, M and Thompson,
WM},
Title = {High-resolution multiplanar CT images of the
larynx.},
Journal = {Invest Radiol},
Volume = {17},
Number = {6},
Pages = {634-637},
Year = {1982},
ISSN = {0020-9996},
url = {http://www.ncbi.nlm.nih.gov/pubmed/7152866},
Abstract = {The standard technique for computed tomographic evaluation
of the larynx utilizes 5-mm contiguous transaxial sections.
Multiplanar images reformatted with these sections have not
been of clinical use. We have evaluated the practicality of
utilizing coronal and sagittal reformatted images produced
from contiguous 1.5-mm transaxial sections. The technique of
rapid sequential scanning with automatic table
incrementation allows 36 contiguous thin section scans to be
acquired in less than 9 minutes. Phantom studies showed a
marked improvement in spatial resolution with thin section
reconstructions. Preliminary clinical evaluation shows
visualization of smaller structures with improved edge
definition of both low- and high-contrast
structures.},
Doi = {10.1097/00004424-198211000-00019},
Key = {Silverman82}
}
@article{fds132770,
Author = {PM Silverman and GA Johnson and M Korobkin and WM
Thompson},
Title = {High-resolution multiplanar CT images of the
larynx.},
Journal = {Investigative radiology, UNITED STATES},
Volume = {17},
Number = {6},
Pages = {634-7},
ISSN = {0020-9996},
Keywords = {Humans • Larynx • Models, Structural •
Tomography, X-Ray Computed • methods* •
radiography*},
Abstract = {The standard technique for computed tomographic evaluation
of the larynx utilizes 5-mm contiguous transaxial sections.
Multiplanar images reformatted with these sections have not
been of clinical use. We have evaluated the practicality of
utilizing coronal and sagittal reformatted images produced
from contiguous 1.5-mm transaxial sections. The technique of
rapid sequential scanning with automatic table
incrementation allows 36 contiguous thin section scans to be
acquired in less than 9 minutes. Phantom studies showed a
marked improvement in spatial resolution with thin section
reconstructions. Preliminary clinical evaluation shows
visualization of smaller structures with improved edge
definition of both low- and high-contrast
structures.},
Key = {fds132770}
}
@article{fds268733,
Author = {O'Leary-Moore, SK and Budin, F and Paniagua, B and Oguz, I and Johnson,
GA and Sulik, KK},
Title = {HIGH-RESOLUTION NEUROIMAGING REVEALS A RANGE OF CORPUS
CALLOSUM INSULT INDUCED BY ETHANOL ON GESTATIONAL DAY 7
INMICE},
Journal = {ALCOHOLISM-CLINICAL AND EXPERIMENTAL RESEARCH},
Volume = {37},
Pages = {167A-167A},
Year = {2013},
ISSN = {0145-6008},
url = {http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000318998300625&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=47d3190e77e5a3a53558812f597b0b92},
Key = {fds268733}
}
@article{fds268813,
Author = {Zhang, X and Badea, C and Hood, G and Wetzel, A and Qi, Y and Stiles, J and Johnson, GA},
Title = {High-resolution reconstruction of fluorescent inclusions in
mouse thorax using anatomically guided sampling and parallel
Monte Carlo computing.},
Journal = {Biomed Opt Express},
Volume = {2},
Number = {9},
Pages = {2449-2460},
Year = {2011},
Month = {September},
ISSN = {2156-7085},
url = {http://www.ncbi.nlm.nih.gov/pubmed/21991539},
Abstract = {We present a method for high-resolution reconstruction of
fluorescent images of the mouse thorax. It features an
anatomically guided sampling method to retrospectively
eliminate problematic data and a parallel Monte Carlo
software package to compute the Jacobian matrix for the
inverse problem. The proposed method was capable of
resolving microliter-sized femtomole amount of quantum dot
inclusions closely located in the middle of the mouse
thorax. The reconstruction was verified against
co-registered micro-CT data. Using the proposed method, the
new system achieved significantly higher resolution and
sensitivity compared to our previous system consisting of
the same hardware. This method can be applied to any system
utilizing similar imaging principles to improve imaging
performance.},
Language = {eng},
Doi = {10.1364/BOE.2.002449},
Key = {fds268813}
}
@article{fds132867,
Author = {PM Silverman and GA Johnson and M Korobkin},
Title = {High-resolution sagittal and coronal reformatted CT images
of the larynx.},
Journal = {AJR. American journal of roentgenology, UNITED
STATES},
Volume = {140},
Number = {4},
Pages = {819-22},
Year = {1983},
Month = {April},
ISSN = {0361-803X},
Keywords = {Computers • Humans • Laryngeal Neoplasms •
Larynx • Time Factors • Tomography, X-Ray Computed
• methods* • radiography* •
radiotherapy},
Key = {fds132867}
}
@booklet{Silverman83,
Author = {Silverman, PM and Johnson, GA and Korobkin, M},
Title = {High-resolution sagittal and coronal reformatted CT images
of the larynx.},
Journal = {AJR Am J Roentgenol},
Volume = {140},
Number = {4},
Pages = {819-822},
Year = {1983},
Month = {April},
ISSN = {0361-803X},
url = {http://www.ncbi.nlm.nih.gov/pubmed/6601398},
Doi = {10.2214/ajr.140.4.819},
Key = {Silverman83}
}
@article{fds268859,
Author = {Johnson, GA and Ali-Sharief, A and Badea, A and Brandenburg, J and Cofer, G and Fubara, B and Gewalt, S and Hedlund, LW and Upchurch,
L},
Title = {High-throughput morphologic phenotyping of the mouse brain
with magnetic resonance histology.},
Journal = {Neuroimage},
Volume = {37},
Number = {1},
Pages = {82-89},
Year = {2007},
Month = {August},
ISSN = {1053-8119},
url = {http://www.ncbi.nlm.nih.gov/pubmed/17574443},
Keywords = {Animals • Brain • Databases as Topic •
Dominance, Cerebral • Image Enhancement • Image
Processing, Computer-Assisted • Imaging,
Three-Dimensional • Magnetic Resonance Imaging •
Mice • Mice, Inbred C57BL • Phenotype* •
Sensitivity and Specificity • Software* • anatomy
& histology* • methods* • physiology},
Abstract = {The Mouse Biomedical Informatics Research Network (MBIRN)
has been established to integrate imaging studies of the
mouse brain ranging from three-dimensional (3D) studies of
the whole brain to focused regions at a sub-cellular scale.
Magnetic resonance (MR) histology provides the entry point
for many morphologic comparisons of the whole brain. We
describe a standardized protocol that allows acquisition of
3D MR histology (43-microm resolution) images of the fixed,
stained mouse brain with acquisition times <30 min. A higher
resolution protocol with isotropic spatial resolution of
21.5 microm can be executed in 2 h. A third acquisition
protocol provides an alternative image contrast (at
43-microm isotropic resolution), which is exploited in a
statistically driven algorithm that segments 33 of the most
critical structures in the brain. The entire process, from
specimen perfusion, fixation and staining, image acquisition
and reconstruction, post-processing, segmentation,
archiving, and analysis, is integrated through a structured
workflow. This yields a searchable database for archive and
query of the very large (1.2 GB) images acquired with this
standardized protocol. These methods have been applied to a
collection of both male and female adult murine brains
ranging over 4 strains and 6 neurologic knockout models.
These collection and acquisition methods are now available
to the neuroscience community as a standard web-deliverable
service.},
Doi = {10.1016/j.neuroimage.2007.05.013},
Key = {fds268859}
}
@article{fds132872,
Author = {HP McAdams and GA Johnson and SA Suddarth and CE Ravin},
Title = {Histogram-directed processing of digital chest
images.},
Journal = {Investigative radiology, UNITED STATES},
Volume = {21},
Number = {3},
Pages = {253-9},
Year = {1986},
Month = {March},
ISSN = {0020-9996},
Keywords = {Computers • Humans • Lasers • Lung •
Mediastinum • Radiographic Image Enhancement •
Radiography, Thoracic • methods* •
radiography},
Abstract = {One of the potential advantages of digital chest imaging is
the ability to process these images. However, such
processing, when uniformly applied to the entire image, is
often unsatisfactory due to the different processing
requirements of lung field and mediastinum. Therefore, a
method to selectively process these regions based upon the
histogram of the original image has been developed. Thirteen
conventional chest films were digitized with a laser film
scanner. Analysis of individual lung field and mediastinum
histograms showed that the chest image histogram is
essentially bimodal with significant lung field-mediastinum
histogram peak separation; overlap between these peaks is
small (9% of the total histogram) and insensitive to minor
pathologic change or radiographic technique. Using these
histograms, a gray level threshold distinguishing
mediastinum from lung field was selected and used to direct
the regionally-selective processing of several chest images.
This technique may prove especially useful for digital
enhancement of the underexposed mediastinum often
encountered on conventional chest radiographs.},
Key = {fds132872}
}
@booklet{Mcadams86,
Author = {McAdams, HP and Johnson, GA and Suddarth, SA and Ravin,
CE},
Title = {Histogram-directed processing of digital chest
images.},
Journal = {Invest Radiol},
Volume = {21},
Number = {3},
Pages = {253-259},
Year = {1986},
Month = {March},
ISSN = {0020-9996},
url = {http://www.ncbi.nlm.nih.gov/pubmed/3957599},
Abstract = {One of the potential advantages of digital chest imaging is
the ability to process these images. However, such
processing, when uniformly applied to the entire image, is
often unsatisfactory due to the different processing
requirements of lung field and mediastinum. Therefore, a
method to selectively process these regions based upon the
histogram of the original image has been developed. Thirteen
conventional chest films were digitized with a laser film
scanner. Analysis of individual lung field and mediastinum
histograms showed that the chest image histogram is
essentially bimodal with significant lung field-mediastinum
histogram peak separation; overlap between these peaks is
small (9% of the total histogram) and insensitive to minor
pathologic change or radiographic technique. Using these
histograms, a gray level threshold distinguishing
mediastinum from lung field was selected and used to direct
the regionally-selective processing of several chest images.
This technique may prove especially useful for digital
enhancement of the underexposed mediastinum often
encountered on conventional chest radiographs.},
Doi = {10.1097/00004424-198603000-00011},
Key = {Mcadams86}
}
@article{fds132878,
Author = {GA Johnson and H Benveniste and RD Black and LW Hedlund and RR Maronpot and BR Smith},
Title = {Histology by magnetic resonance microscopy.},
Journal = {Magnetic resonance quarterly, UNITED STATES},
Volume = {9},
Number = {1},
Pages = {1-30},
Year = {1993},
Month = {March},
ISSN = {0899-9422},
Keywords = {Animals • Histological Techniques • Histology*
• Image Enhancement • Magnetic Resonance Imaging
• Microscopy • Tissue Fixation •
instrumentation • methods*},
Abstract = {Magnetic resonance microscopy (MRM) has advanced from a
technical challenge to a practical tool in a wide range of
basic sciences. This article focuses on the use of MRM as a
tool for histological studies. The technical challenges of
limited signal to noise have been overcome by improved
radio-frequency (rf) coil design and 3DFT encoding with
large arrays. Resolution limits imposed by motion in in vivo
studies have been overcome by improved physiologic
monitoring and control and projection encoding. Integration
of technologies now permits routine studies in vivo down to
50 microns. MRM has also been applied to in vitro studies of
fixed tissues where absence of motion allows studies down to
10 microns. The nondestructive nature of the technique
allows repeated studies of the same sample, retrospective
studies through any arbitrary plane, registered studies
using different contrast mechanisms, and examination of
valuable specimens. The many and unique proton contrasts
provided by MRM, i.e., T1, T2, and diffusion weighting,
permit direct examination of the state of water in tissues,
something not possible with other microscopic techniques.
Finally, the inherent three-dimensional nature of MRM allows
acquisition of perfectly registered isotropic 3D arrays
that, when displayed with appropriate visualization tools,
provide new perspectives to histologic examination. The
technology of MRM continues to develop rapidly. New pulse
sequences are reducing acquisition times. New computer
architectures allow larger arrays. A new class of
superconducting rf probe has increased the signal to noise
ratio by 10 times. These developments promise routine use of
MRM in histology studies with resolution to 1 micron in the
near future.},
Key = {fds132878}
}
@booklet{Johnson93,
Author = {Johnson, GA and Benveniste, H and Black, RD and Hedlund, LW and Maronpot, RR and Smith, BR},
Title = {Histology by magnetic resonance microscopy.},
Journal = {Magn Reson Q},
Volume = {9},
Number = {1},
Pages = {1-30},
Year = {1993},
Month = {March},
ISSN = {0899-9422},
url = {http://www.ncbi.nlm.nih.gov/pubmed/8512830},
Abstract = {Magnetic resonance microscopy (MRM) has advanced from a
technical challenge to a practical tool in a wide range of
basic sciences. This article focuses on the use of MRM as a
tool for histological studies. The technical challenges of
limited signal to noise have been overcome by improved
radio-frequency (rf) coil design and 3DFT encoding with
large arrays. Resolution limits imposed by motion in in vivo
studies have been overcome by improved physiologic
monitoring and control and projection encoding. Integration
of technologies now permits routine studies in vivo down to
50 microns. MRM has also been applied to in vitro studies of
fixed tissues where absence of motion allows studies down to
10 microns. The nondestructive nature of the technique
allows repeated studies of the same sample, retrospective
studies through any arbitrary plane, registered studies
using different contrast mechanisms, and examination of
valuable specimens. The many and unique proton contrasts
provided by MRM, i.e., T1, T2, and diffusion weighting,
permit direct examination of the state of water in tissues,
something not possible with other microscopic techniques.
Finally, the inherent three-dimensional nature of MRM allows
acquisition of perfectly registered isotropic 3D arrays
that, when displayed with appropriate visualization tools,
provide new perspectives to histologic examination. The
technology of MRM continues to develop rapidly. New pulse
sequences are reducing acquisition times. New computer
architectures allow larger arrays. A new class of
superconducting rf probe has increased the signal to noise
ratio by 10 times. These developments promise routine use of
MRM in histology studies with resolution to 1 micron in the
near future.},
Key = {Johnson93}
}
@booklet{Green80,
Author = {D. J. Green and G. A. Johnson and S. T. Hutchison},
Title = {Hot-pressing sodium beta-alumina using solution spray-dried
and freeze-dried powders},
Journal = {Journal Of The Canadian Ceramic Society},
Volume = {49},
Pages = {7 -- 12},
Year = {1980},
Key = {Green80}
}
@article{fds174078,
Author = {GA Johnson and PA Van Pernis},
Title = {How pathology kept pace with expansion.},
Journal = {Modern hospital},
Volume = {105},
Number = {5},
Pages = {105-7},
Year = {1965},
Month = {November},
ISSN = {0026-783X},
Keywords = {Hospital Design and Construction* • Humans •
Illinois • Laboratories* • Pathology*},
Language = {eng},
Key = {fds174078}
}
@article{fds132793,
Author = {JR MacFall and HC Charles and RD Black and H Middleton and JC Swartz and B
Saam, B Driehuys and C Erickson and W Happer and GD Cates and GA
Johnson, CE Ravin},
Title = {Human lung air spaces: potential for MR imaging with
hyperpolarized He-3.},
Journal = {Radiology, UNITED STATES},
Volume = {200},
Number = {2},
Pages = {553-8},
Year = {1996},
Month = {August},
ISSN = {0033-8419},
Keywords = {Adult • Helium • Humans • Image Enhancement
• Isotopes • Lung • Magnetic Resonance
Imaging • Male • Middle Aged • anatomy &
histology* • diagnostic use* • instrumentation
• methods • methods*},
Abstract = {Two healthy volunteers who had inhaled approximately 0.75 L
of laser-polarized helium-3 gas underwent magnetic resonance
imaging at 1.5 T with fast gradient-echo pulse sequences and
small flip angles ( < 10 degrees). Thick-section (20 mm)
coronal images, time-course data (30 images collected every
1.8 seconds), and thin-section (6 mm) images were acquired.
Subjects were able to breathe the gas (12% polarization)
without difficulty. Thick-section images were of good
quality and had a signal-to-noise ratio (S/N) of 32:1 near
the surface coil and 16:1 farther away. The time images
showed regional differences, which indicated potential value
for quantitation. High-resolution images showed greater
detail and a S/N of approximately 6:1.},
Key = {fds132793}
}
@booklet{Macfall96,
Author = {MacFall, JR and Charles, HC and Black, RD and Middleton, H and Swartz,
JC and Saam, B and Driehuys, B and Erickson, C and Happer, W and Cates, GD and Johnson, GA and Ravin, CE},
Title = {Human lung air spaces: potential for MR imaging with
hyperpolarized He-3.},
Journal = {Radiology},
Volume = {200},
Number = {2},
Pages = {553-558},
Year = {1996},
Month = {August},
ISSN = {0033-8419},
url = {http://www.ncbi.nlm.nih.gov/pubmed/8685356},
Abstract = {Two healthy volunteers who had inhaled approximately 0.75 L
of laser-polarized helium-3 gas underwent magnetic resonance
imaging at 1.5 T with fast gradient-echo pulse sequences and
small flip angles ( < 10 degrees). Thick-section (20 mm)
coronal images, time-course data (30 images collected every
1.8 seconds), and thin-section (6 mm) images were acquired.
Subjects were able to breathe the gas (12% polarization)
without difficulty. Thick-section images were of good
quality and had a signal-to-noise ratio (S/N) of 32:1 near
the surface coil and 16:1 farther away. The time images
showed regional differences, which indicated potential value
for quantitation. High-resolution images showed greater
detail and a S/N of approximately 6:1.},
Doi = {10.1148/radiology.200.2.8685356},
Key = {Macfall96}
}
@booklet{Kudlacek95,
Author = {P. E. Kudlacek and R. J. Anderson and D. K. Liebentritt and G. A. Johnson and C. J. Huerter},
Title = {Human skin and platelet minoxidil sulfotransferase
activities - biochemical-properties, correlations and
contribution of thermolabile phenol sulfotransferase},
Journal = {Journal Of Pharmacology And Experimental
Therapeutics},
Volume = {273},
Number = {2},
Pages = {582 -- 590},
Year = {1995},
Month = {May},
Key = {Kudlacek95}
}
@article{fds174118,
Author = {PE Kudlacek and RJ Anderson and DK Liebentritt and GA Johnson and CJ
Huerter},
Title = {Human skin and platelet minoxidil sulfotransferase
activities: biochemical properties, correlations and
contribution of thermolabile phenol sulfotransferase.},
Journal = {The Journal of pharmacology and experimental
therapeutics},
Volume = {273},
Number = {2},
Pages = {582-90},
Year = {1995},
Month = {May},
ISSN = {0022-3565},
Keywords = {Arylsulfotransferase • Blood Platelets •
Chromatography, High Pressure Liquid • Chromatography,
Ion Exchange • Enzyme Stability • Humans •
Minoxidil • Nitrophenols • Skin • Sodium
Chloride • Substrate Specificity •
Sulfotransferases • Temperature • antagonists &
inhibitors • enzymology* • isolation &
purification • metabolism* • pharmacology},
Abstract = {Human scalp skin high speed supernatants were used to test
whether minoxidil sulfotransferase (MNX-ST) and phenol
sulfotransferase (PST) activities were present. Platelet
homogenates from the same skin donors were used to test
whether levels of sulfotransferase activities in the blood
platelet would reflect levels of the enzyme activities in
skin. Dopamine, p-nitrophenol and minoxidil were used as
substrates for skin and platelet thermolabile (TL PST),
thermostable (TS PST) and MNX-ST activities, respectively.
Biochemical properties of each skin enzyme were the same as
the platelet enzymes with respect to apparent Km values for
substrates, pH optima, thermal stabilities and responses to
inhibition by 2,6-dichloro-4-nitrophenol (DCNP). An
unexpected finding was that skin and platelet MNX-ST thermal
stabilities and responses to DCNP were more similar to TL
PST than to TS PST, the enzyme reported to be responsible
for MNX-ST activity. There were significant positive
correlations of platelet sulfotransferases with the relative
levels of activities of the same skin sulfotransferases.
Unexpected findings were significant positive correlations
of MNX-ST and TL PST activities. Partially purified platelet
TS PST assayed with minoxidil as the substrate showed a
response to DCNP and thermal stability that were the same as
TS PST. Platelet TL PST assayed with minoxidil showed
thermal stability and a response to DCNP that were
essentially the same as TL PST. The results indicated that
not only TS PST, but also TL PST activities in human skin
and platelet contributed to MNX-ST activity. It will be
feasible to test whether measures of platelet PST activities
will predict physiologic responses to minoxidil.},
Language = {eng},
Key = {fds174118}
}
@article{fds132745,
Author = {G.A. Johnson},
Title = {Hurlston SE, Brey WW, Suddarth SA, Yap M, Johson GA. A high
temperature superconducting Helmholtz probe for microscopy
at 9.4 T Magn Res Med. In press 1998},
Year = {1998},
Key = {fds132745}
}
@article{fds269056,
Author = {Chawla, MS and Chen, XJ and Cofer, GP and Hedlund, LW and Kerby, MB and Ottoboni, TB and Johnson, GA},
Title = {Hyperpolarized 3He microspheres as a novel vascular signal
source for MRI.},
Journal = {Magn Reson Med},
Volume = {43},
Number = {3},
Pages = {440-445},
Year = {2000},
Month = {March},
ISSN = {0740-3194},
url = {http://www.ncbi.nlm.nih.gov/pubmed/10725887},
Keywords = {Animals • Helium • Image Enhancement •
Magnetic Resonance Angiography* • Male •
Microscopy, Electron, Scanning • Microspheres •
Pelvis • Phantoms, Imaging • Rats • blood
supply • chemistry* • methods},
Abstract = {Hyperpolarized (HP) 3He can be encapsulated within
biologically compatible microspheres while retaining
sufficient polarization to be used as a signal source for
MRI. Two microsphere sizes were used, with mean diameters of
5.3 +/- 1.3 microm and 10.9 +/- 3.0 microm. These
suspensions ranged in concentration from 0.9-7.0% gas by
volume. Spectroscopic measurements in phantoms at 2 T
yielded 3He relaxation times that varied with gas
concentration. At the highest 3He concentration, the
spinlattice relaxation time, T1, was 63.8 +/- 9.4 sec, while
the transverse magnetization decayed with a time constant of
T2* = 11.0 +/- 0.4 msec. In vivo MR images of the pelvic
veins in a rat were acquired during intravenous injection of
3He microspheres (SNR approximately equal 15). Advantages
such as intravascular confinement, lack of background
signal, and limited recirculation indicate quantitative
perfusion measurements may be improved using this novel
signal source.},
Doi = {10.1002/(sici)1522-2594(200003)43:3<440::aid-mrm16>3.0.co;2-},
Key = {fds269056}
}
@article{fds269124,
Author = {Chen, XJ and Chawla, MS and Cofer, GP and Hedlund, LW and Möller, HE and Johnson, GA},
Title = {Hyperpolarized 3He NMR lineshape measurements in the live
guinea pig lung.},
Journal = {Magn Reson Med},
Volume = {40},
Number = {1},
Pages = {61-65},
Year = {1998},
Month = {July},
ISSN = {0740-3194},
url = {http://www.ncbi.nlm.nih.gov/pubmed/9660554},
Keywords = {Animals • Guinea Pigs • Helium • Isotopes
• Lung • Magnetic Resonance Spectroscopy •
Pulmonary Gas Exchange • Reference Values •
Respiratory Mechanics • Sensitivity and Specificity
• anatomy & histology* • diagnostic use* •
methods*},
Abstract = {Spatially localized lineshapes of hyperpolarized (HP) 3He in
guinea pig lungs have been measured in vivo. Three different
axial slice locations, each containing different
compositions of airway sizes and orientations, were studied.
Gas peaks from major bronchi (2 ppm) and alveoli (-2 ppm)
were distinguished. The gas phase spectra show structural
features that are a result of frequency shifts caused by
bulk magnetic susceptibility. For a given slice, the
spectral lineshapes reflect the airway composition within
the slice location, according to theory. The peak
assignments given here also agree with previous studies done
by Wagshul et al. with HP 129Xe. At each of the slice
locations, data were acquired during two phases of the
breathing cycle, resulting in a relative frequency shift of
approximately 0.3 ppm in the superior slices. Spectra
obtained over a number of breaths show the dynamics of the
gas buildup in the lung and provide further evidence
supporting the peak assignments.},
Doi = {10.1002/mrm.1910400109},
Key = {fds269124}
}
@booklet{Chawla00,
Author = {M. S. Chawla and X. J. Chen and G. P. Cofer and L. W.
Hedlund and M. B. Kerby and T. B. Ottoboni and G. A.
Johnson},
Title = {Hyperpolarized He-3 microspheres as a novel vascular signal
source for MRI},
Journal = {Magnetic Resonance In Medicine},
Volume = {43},
Number = {3},
Pages = {440 -- 445},
Year = {2000},
Month = {March},
Key = {Chawla00}
}
@booklet{Chen98,
Author = {X. J. Chen and M. S. Chawla and G. P. Cofer and L. W.
Hedlund and H. E. Moller and G. A. Johnson},
Title = {Hyperpolarized He-3 NMR lineshape measurements in the live
guinea pig lung},
Journal = {Magnetic Resonance In Medicine},
Volume = {40},
Number = {1},
Pages = {61 -- 65},
Year = {1998},
Month = {July},
Key = {Chen98}
}
@article{fds174086,
Author = {GA Johnson},
Title = {HYPOCHLORITE TREATMENT OF PUBLIC WATER SUPPLIES: ITS
ADAPTIBILITY AND LIMITATIONS.},
Journal = {Journal. American Public Health Association},
Volume = {1},
Number = {8},
Pages = {562-74},
Year = {1911},
Month = {August},
ISSN = {0273-1975},
Language = {eng},
Key = {fds174086}
}
@article{fds174158,
Author = {GA Johnson},
Title = {HYPOCHLORITE TREATMENT OF PUBLIC WATER SUPPLIES: ITS
ADAPTIBILITY AND LIMITATIONS.},
Journal = {Journal. American Public Health Association},
Volume = {1},
Number = {8},
Pages = {562-574},
Year = {1911},
Month = {August},
Language = {ENG},
Key = {fds174158}
}
@booklet{Spencer99c,
Author = {T. E. Spencer and F. F. Bartol and F. W. Bazer and G. A.
Johnson and M. M. Joyce},
Title = {Identification and characterization of glycosylation-dependent
cell adhesion molecule 1-like protein expression in the
ovine uterus},
Journal = {Biology Of Reproduction},
Volume = {60},
Number = {2},
Pages = {241 -- 250},
Year = {1999},
Month = {February},
Key = {Spencer99c}
}
@article{fds174088,
Author = {TE Spencer and FF Bartol and FW Bazer and GA Johnson and MM
Joyce},
Title = {Identification and characterization of glycosylation-dependent
cell adhesion molecule 1-like protein expression in the
ovine uterus.},
Journal = {Biology of reproduction},
Volume = {60},
Number = {2},
Pages = {241-50},
Year = {1999},
Month = {February},
ISSN = {0006-3363},
Keywords = {Animals • Blotting, Western • Embryonic
Development • Endometrium • Epithelium •
Estrus • Female • Gestational Age •
Immunosorbent Techniques • Mucins • Myometrium
• Pregnancy • Sheep • Uterus • analysis*
• chemistry • chemistry*},
Abstract = {Glycosylation-dependent cell adhesion molecule 1 (GlyCAM-1)
is an endothelial glycoprotein secreted in lymph nodes that
serves as a ligand for leukocyte cell surface selectin and
mediates lymphocyte extravasation. In the present studies,
rabbit anti-rat GlyCAM-1 IgG was used in immunochemical
analyses of GlyCAM-1-like protein in the ovine uterus. In
cyclic ewes, GlyCAM-1 expression increased in the
endometrial luminal epithelium (LE) and shallow glandular
epithelium (cGE) between Days 1 and 5 and then decreased
between Days 11 and 15. In pregnant ewes, GlyCAM-1 in the LE
and cGE was low on Days 11 and 13, increased on Day 15, and
was abundant on Days 17 and 19. Immunoreactive GlyCAM-1 was
also detected in the conceptus trophectoderm on Days 13-19.
Staining for GlyCAM-1 in the smooth muscle of the
vasculature and myometrium was constitutive, and no staining
was detected in the stroma. An immunoreactive protein of
approximately 45 kDa was identified in endometrial extracts
and uterine flushings from cyclic and pregnant ewes. In
pregnant ewes, the relative amount of immunoreactive
GlyCAM-1 in uterine flushings was low on Days 11 and 13 but
high on Days 15 and 17. Results suggest that a GlyCAM-1-like
protein may be a secretory product of the endometrial
epithelium and/or conceptus trophectoderm. Patterns of
distribution observed for immunoreactive GlyCAM-1-like
protein in the endometrial epithelium, combined with
proposed functions for lymphoid GlyCAM-1, suggest that this
mucin glycoprotein may be involved in conceptus-maternal
interactions during the periimplantation period of pregnancy
in sheep.},
Language = {eng},
Key = {fds174088}
}
@article{fds357210,
Author = {Badea, A and Wu, W and Shuff, J and Wang, M and Anderson, RJ and Qi, Y and Johnson, GA and Wilson, JG and Koudoro, S and Garyfallidis, E and Colton, CA and Dunson, DB},
Title = {Identifying Vulnerable Brain Networks in Mouse Models of
Genetic Risk Factors for Late Onset Alzheimer's
Disease.},
Journal = {Front Neuroinform},
Volume = {13},
Pages = {72},
Year = {2019},
url = {http://dx.doi.org/10.3389/fninf.2019.00072},
Abstract = {The major genetic risk for late onset Alzheimer's disease
has been associated with the presence of APOE4 alleles.
However, the impact of different APOE alleles on the brain
aging trajectory, and how they interact with the brain local
environment in a sex specific manner is not entirely clear.
We sought to identify vulnerable brain circuits in novel
mouse models with homozygous targeted replacement of the
mouse ApoE gene with either human APOE3 or APOE4 gene
alleles. These genes are expressed in mice that also model
the human immune response to age and disease-associated
challenges by expressing the human NOS2 gene in place of the
mouse mNos2 gene. These mice had impaired learning and
memory when assessed with the Morris water maze (MWM) and
novel object recognition (NOR) tests. Ex vivo MRI-DTI
analyses revealed global and local atrophy, and areas of
reduced fractional anisotropy (FA). Using tensor network
principal component analyses for structural connectomes, we
inferred the pairwise connections which best separate APOE4
from APOE3 carriers. These involved primarily
interhemispheric connections among regions of olfactory
areas, the hippocampus, and the cerebellum. Our results also
suggest that pairwise connections may be subdivided and
clustered spatially to reveal local changes on a finer
scale. These analyses revealed not just genotype, but also
sex specific differences. Identifying vulnerable networks
may provide targets for interventions, and a means to
stratify patients.},
Doi = {10.3389/fninf.2019.00072},
Key = {fds357210}
}
@article{fds204260,
Author = {C Abad and DF Antczak and J Carvalho and LW Chamley and Q Chen and S Daher and AE Damiano and V Dantzer and P Díaz and CE Dunk and E Daly and C Escudero and B Falcón and M Guillomot and YW Han and LK Harris and JP Huidobro-Toro and N Illsley and H Jammes and T Jansson and GA Johnson, JR Kfoury Jr and R
Marín, P Murthi and B Novakovic and L Myatt and MG Petroff and FT
Pereira, C Pfarrer and CW Redman and G Rice and R Saffery and JM Tolosa and C Vaillancourt and M Wareing and R Yuen and GE Lash},
Title = {IFPA Meeting 2010 Workshop Report I: Immunology; ion
transport; epigenetics; vascular reactivity;
epitheliochorial placentation; proteomics.},
Journal = {Placenta},
Volume = {32 Suppl 2},
Pages = {S81-9},
Year = {2011},
Month = {March},
ISSN = {1532-3102},
url = {http://dx.doi.org/10.1016/j.placenta.2010.12.019},
Keywords = {Animals • Education • Epigenesis, Genetic •
Female • Fetus • Humans • Ion Transport
• Maternal-Fetal Exchange • Placenta •
Placentation • Pregnancy • Proteomics •
Trophoblasts • blood supply • cytology •
immunology • methods • physiology •
physiology*},
Abstract = {Workshops are an important part of the IFPA annual meeting.
At IFPA Meeting 2010 there were twelve themed workshops, six
of which are summarized in this report. 1. The immunology
workshop focused on normal and pathological functions of the
maternal immune system in pregnancy. 2. The transport
workshop dealt with regulation of ion and water transport
across the syncytiotrophoblast of human placenta. 3. The
epigenetics workshop covered DNA methylation and its
potential role in regulating gene expression in placental
development and disease. 4. The vascular reactivity workshop
concentrated on methodological approaches used to study
placental vascular function. 5. The workshop on
epitheliochorial placentation covered current advances from
in vivo and in vitro studies of different domestic species.
6. The proteomics workshop focused on a variety of
techniques and procedures necessary for proteomic analysis
and how they may be implemented for placental
research.},
Language = {eng},
Doi = {10.1016/j.placenta.2010.12.019},
Key = {fds204260}
}
@booklet{Johnson02e,
Author = {Johnson, GA and Hedlund, LW},
Title = {Image based phenotyping: The visible mouse},
Journal = {FASEB JOURNAL},
Volume = {16},
Number = {5},
Pages = {A1091-A1091},
Year = {2002},
ISSN = {0892-6638},
url = {http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000174593902021&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=47d3190e77e5a3a53558812f597b0b92},
Key = {Johnson02e}
}
@article{fds268888,
Author = {Sherrier, RH and Chotas, HG and Johnson, GA and Chiles, C and Ravin,
CE},
Title = {Image optimization in a computed-radiography/photostimulable-phosphor
system.},
Journal = {J Digit Imaging},
Volume = {2},
Number = {4},
Pages = {212-219},
Year = {1989},
Month = {November},
ISSN = {0897-1889},
url = {http://www.ncbi.nlm.nih.gov/pubmed/2488166},
Keywords = {Humans • Image Processing, Computer-Assisted* •
Radiography, Thoracic • Tomography, X-Ray Computed*
• X-Ray Film},
Abstract = {Photostimulable phosphor imaging is an exciting new
technology that has several advantages over film/screen
radiography, the most important of which is the linearity of
the photostimulable phosphor system over a wide exposure
latitude. The photostimulable phosphor image is digital, and
as such, provides options of how the image is viewed by
radiologists. This report discusses the various
image-processing parameters available for a photostimulable
phosphor system and describes a rational approach for
selecting these parameters in portable chest radiography. As
photostimulable phosphor imaging becomes more widely
implemented, an understanding of the processing parameters
will facilitate the production of images that take full
advantage of the benefits of these systems.},
Doi = {10.1007/BF03170408},
Key = {fds268888}
}
@article{fds132851,
Author = {GA Johnson and M Korobkin},
Title = {Image techniques for multiplanar computed
tomography.},
Journal = {Radiology, UNITED STATES},
Volume = {144},
Number = {4},
Pages = {829-34},
Year = {1982},
Month = {September},
ISSN = {0033-8419},
Keywords = {Humans • Models, Structural • Radiographic Image
Enhancement • Technology, Radiologic • Tomography,
X-Ray Computed • methods*},
Abstract = {Hardware and software options of the GE 8800 CT scanner were
analyzed with respect to their effect on image quality for
multiplanar images. Phantom studies were undertaken to
demonstrate the effect of collimation, thick pixel
reconstruction, and interpolation of both high- and
low-contrast multiplanar images. Noise and spatial
resolution were measured. Thick pixel reconstruction was
found to be most useful in aiding in the delineation of
low-contrast lesion boundaries. In addition, this option
permits use of lower techniques, thus speeding data
acquisition and reducing patient dose. Clinical examples are
included.},
Key = {fds132851}
}
@booklet{Johnson82e,
Author = {Johnson, GA and Korobkin, M},
Title = {Image techniques for multiplanar computed
tomography.},
Journal = {Radiology},
Volume = {144},
Number = {4},
Pages = {829-834},
Year = {1982},
Month = {September},
ISSN = {0033-8419},
url = {http://www.ncbi.nlm.nih.gov/pubmed/7111733},
Abstract = {Hardware and software options of the GE 8800 CT scanner were
analyzed with respect to their effect on image quality for
multiplanar images. Phantom studies were undertaken to
demonstrate the effect of collimation, thick pixel
reconstruction, and interpolation of both high- and
low-contrast multiplanar images. Noise and spatial
resolution were measured. Thick pixel reconstruction was
found to be most useful in aiding in the delineation of
low-contrast lesion boundaries. In addition, this option
permits use of lower techniques, thus speeding data
acquisition and reducing patient dose. Clinical examples are
included.},
Doi = {10.1148/radiology.144.4.7111733},
Key = {Johnson82e}
}
@article{fds320200,
Author = {Johnson, GA and Anderson, RJ and Cook, JJ and Long, C and Badea,
A},
Title = {Image-processing pipelines: Applications in magnetic
resonance histology},
Journal = {Progress in Biomedical Optics and Imaging - Proceedings of
SPIE},
Volume = {9784},
Publisher = {SPIE},
Year = {2016},
Month = {January},
ISBN = {9781510600195},
url = {http://dx.doi.org/10.1117/12.2203525},
Abstract = {Image processing has become ubiquitous in imaging research -
so ubiquitous that it is easy to loose track of how diverse
this processing has become. The Duke Center for In Vivo
Microscopy has pioneered the development of Magnetic
Resonance Histology (MRH), which generates large
multidimensional data sets that can easily reach into the
tens of gigabytes. A series of dedicated image-processing
workstations and associated software have been assembled to
optimize each step of acquisition, reconstruction,
post-processing, registration, visualization, and
dissemination. This talk will describe the image-processing
pipelines from acquisition to dissemination that have become
critical to our everyday work.},
Doi = {10.1117/12.2203525},
Key = {fds320200}
}
@booklet{Yelbuz03,
Author = {Yelbuz, TM and Zhang, X and Choma, MA and Stadt, HA and Zdanowicz, M and Johnson, GA and Kirby, ML},
Title = {Images in cardiovascular medicine. Approaching cardiac
development in three dimensions by magnetic resonance
microscopy.},
Journal = {Circulation},
Volume = {108},
Number = {22},
Pages = {e154-e155},
Year = {2003},
Month = {December},
ISSN = {0009-7322},
url = {http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000186894500017&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=47d3190e77e5a3a53558812f597b0b92},
Doi = {10.1161/01.CIR.0000102940.17908.CA},
Key = {Yelbuz03}
}
@article{fds268862,
Author = {Driehuys, B and Cofer, GP and Pollaro, J and Mackel, JB and Hedlund, LW and Johnson, GA},
Title = {Imaging alveolar-capillary gas transfer using hyperpolarized
129Xe MRI.},
Journal = {Proc Natl Acad Sci U S A},
Volume = {103},
Number = {48},
Pages = {18278-18283},
Year = {2006},
Month = {November},
ISSN = {0027-8424},
url = {http://www.ncbi.nlm.nih.gov/pubmed/17101964},
Keywords = {Animals • Erythrocytes • Magnetic Resonance
Imaging • Microcirculation • Oxygen • Rats
• Rats, Inbred F344 • Respiration* • Xenon
Isotopes • metabolism • metabolism* •
methods*},
Abstract = {Effective pulmonary gas exchange relies on the free
diffusion of gases across the thin tissue barrier separating
airspace from the capillary red blood cells (RBCs).
Pulmonary pathologies, such as inflammation, fibrosis, and
edema, which cause an increased blood-gas barrier thickness,
impair the efficiency of this exchange. However, definitive
assessment of such gas-exchange abnormalities is
challenging, because no methods currently exist to directly
image the gas transfer process. Here we exploit the
solubility and chemical shift of (129)Xe, the magnetic
resonance signal of which has been enhanced by 10(5) with
hyperpolarization, to differentially image its transfer from
the airspaces into the tissue barrier spaces and RBCs in the
gas exchange regions of the lung. Based on a simple
diffusion model, we estimate that this MR imaging method for
measuring (129)Xe alveolar-capillary transfer is sensitive
to changes in blood-gas barrier thickness of approximately 5
microm. We validate the successful separation of tissue
barrier and RBC images and show the utility of this method
in a rat model of pulmonary fibrosis where (129)Xe
replenishment of the RBCs is severely impaired in regions of
lung injury.},
Doi = {10.1073/pnas.0608458103},
Key = {fds268862}
}
@article{fds132842,
Author = {PJ Gareau and AC Wymore and GP Cofer and GA Johnson},
Title = {Imaging inflammation: direct visualization of perivascular
cuffing in EAE by magnetic resonance microscopy.},
Journal = {Journal of magnetic resonance imaging : JMRI, United
States},
Volume = {16},
Number = {1},
Pages = {28-36},
Year = {2002},
Month = {July},
ISSN = {1053-1807},
Keywords = {Animals • Brain • Disease Models, Animal •
Encephalomyelitis, Autoimmune, Experimental • Female
• Guinea Pigs • Inflammation • Magnetic
Resonance Imaging • Microscopy • Multiple
Sclerosis • methods* • pathology •
pathology*},
Abstract = {PURPOSE: To determine if the architectural features revealed
by magnetic resonance microscopy (MRM) allow one to detect
microscopic abnormalities associated with neuroinflammation
in fixed brain sections from animals with experimental
allergic encephalomyelitis (EAE), an animal model for
multiple sclerosis (MS). MATERIALS AND METHODS: Imaging was
performed at the Center for In Vivo Microscopy (CIVM) using
a 9.4-Tesla, 89-mm bore, superconducting magnet with
actively shielded gradients capable of 850 mT/m. A number of
MR contrasts and spatial resolutions were explored. RESULTS:
The assessment of EAE brain showed that it is possible to
visualize perivascular cuffing in vitro by MRM on
three-dimensional T1 proton stains. CONCLUSION: Inflammatory
cell infiltration is a prerequisite for the development of
lesions in EAE and MS. Thus, the ability to directly detect
individual perivascular cuffs of inflammation may provide a
useful means of monitoring the time course of inflammatory
events, as conventional histopathological scoring of
perivascular cuffs is utilized, but in the absence of
sectioning and staining.},
Key = {fds132842}
}
@booklet{Gareau02,
Author = {Gareau, PJ and Wymore, AC and Cofer, GP and Johnson,
GA},
Title = {Imaging inflammation: direct visualization of perivascular
cuffing in EAE by magnetic resonance microscopy.},
Journal = {J Magn Reson Imaging},
Volume = {16},
Number = {1},
Pages = {28-36},
Year = {2002},
Month = {July},
ISSN = {1053-1807},
url = {http://www.ncbi.nlm.nih.gov/pubmed/12112500},
Abstract = {PURPOSE: To determine if the architectural features revealed
by magnetic resonance microscopy (MRM) allow one to detect
microscopic abnormalities associated with neuroinflammation
in fixed brain sections from animals with experimental
allergic encephalomyelitis (EAE), an animal model for
multiple sclerosis (MS). MATERIALS AND METHODS: Imaging was
performed at the Center for In Vivo Microscopy (CIVM) using
a 9.4-Tesla, 89-mm bore, superconducting magnet with
actively shielded gradients capable of 850 mT/m. A number of
MR contrasts and spatial resolutions were explored. RESULTS:
The assessment of EAE brain showed that it is possible to
visualize perivascular cuffing in vitro by MRM on
three-dimensional T1 proton stains. CONCLUSION: Inflammatory
cell infiltration is a prerequisite for the development of
lesions in EAE and MS. Thus, the ability to directly detect
individual perivascular cuffs of inflammation may provide a
useful means of monitoring the time course of inflammatory
events, as conventional histopathological scoring of
perivascular cuffs is utilized, but in the absence of
sectioning and staining.},
Doi = {10.1002/jmri.10136},
Key = {Gareau02}
}
@booklet{Johnson81d,
Author = {Johnson, GA and Nelson, CE and OʼFoghludha, F},
Title = {IMAGING LIMITS IN DIGITAL RADIOGRAPHY},
Journal = {Investigative Radiology},
Volume = {16},
Number = {5},
Pages = {377-377},
Publisher = {Ovid Technologies (Wolters Kluwer Health)},
Year = {1981},
Month = {September},
ISSN = {0020-9996},
url = {http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:A1981MK56200033&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=47d3190e77e5a3a53558812f597b0b92},
Doi = {10.1097/00004424-198109000-00033},
Key = {Johnson81d}
}
@article{fds268855,
Author = {Badea, CT and Bucholz, E and Hedlund, LW and Rockman, HA and Johnson,
GA},
Title = {Imaging methods for morphological and functional phenotyping
of the rodent heart.},
Journal = {Toxicol Pathol},
Volume = {34},
Number = {1},
Pages = {111-117},
Year = {2006},
ISSN = {0192-6233},
url = {http://www.ncbi.nlm.nih.gov/pubmed/16507552},
Keywords = {Animals • Biological Markers • Heart •
Magnetic Resonance Imaging • Mice •
Microradiography • Myocardium • Rats •
Tomography, X-Ray Computed • methods* • pathology*
• radiography*},
Abstract = {Small animal imaging has a critical role in phenotyping,
drug discovery, and in providing a basic understanding of
mechanisms of disease. Translating imaging methods from
humans to small animals is not an easy task. The purpose of
this work is to compare two cardiac imaging modalities,
i.e., magnetic resonance microscopy (MRM) and microcomputed
tomography (CT) for preclinical studies on rodents. We
present the two technologies, the parameters that they can
measure, the types of alterations that they can detect, and
show how these imaging methods compare to techniques
available in clinical medicine. While this paper does not
refer per se to the cardiac risk assessment for drug or
chemical development, we hope that the information will
effectively address how MRM and micro-CT might be exploited
to measure biomarkers critical for safety
assessment.},
Doi = {10.1080/01926230500404126},
Key = {fds268855}
}
@article{fds268782,
Author = {Johnson, K and Badea, C and Hedlund, L and Johnson,
GA},
Title = {Imaging techniques for small animal imaging models of
pulmonary disease: Micro-CT (Toxicologic Pathology (2007)
35, 5 (9-64))},
Journal = {Toxicologic Pathology},
Volume = {36},
Number = {6},
Pages = {895},
Publisher = {SAGE Publications},
Year = {2008},
Month = {October},
ISSN = {0192-6233},
url = {http://dx.doi.org/10.1177/0192623308323921},
Doi = {10.1177/0192623308323921},
Key = {fds268782}
}
@article{fds132818,
Author = {MM Henson and OW Henson and SL Gewalt and JL Wilson and GA
Johnson},
Title = {Imaging the cochlea by magnetic resonance
microscopy.},
Journal = {Hearing research, NETHERLANDS},
Volume = {75},
Number = {1-2},
Pages = {75-80},
Year = {1994},
Month = {May},
ISSN = {0378-5955},
Keywords = {Animals • Chiroptera • Cochlea • Magnetic
Resonance Imaging* • Scala Tympani • Spiral
Ganglion • Tissue Fixation • Vestibular Nerve
• Vestibulocochlear Nerve • anatomy & histology
• anatomy & histology*},
Abstract = {The isolated, fixed cochlea of the mustached bat was studied
with three dimensional magnetic resonance (MR) microscopy.
The cochlea of this animal is about 4 mm in diameter and its
entire volume was imaged. With the field of view and matrix
size used, the volume elements (voxels) making up the volume
data set were isotropic 25 x 25 x 25 micron cubes. Three
dimensional (3D) MR microscopy based on isotropic voxels has
many advantages over commonly used light microscopy: 1) it
is non destructive; 2) it is much less time consuming; 3) no
dehydration is required and shrinkage is minimized; 4) the
data set can be used to create sections in any desired
plane; 5) the proper alignment of sections is inherent in
the 3D acquisition so that no reference points are required;
6) the entire data set can be viewed from any point of view
in a volume rendered image; 7) the data is digital and
features can be enhanced by computer image processing; and
8) the isotropic dimensions of the voxels make the data
well-suited for structural reconstructions and measurements.
Good images of the osseous spiral lamina, spiral ligament,
scala tympani, scala vestibuli, and nerve bundles were
obtained. The vestibular (Reissner's) membrane was easily
identified in the mustached bat and it appears to bulge into
the scala vestibuli. The visibility of this structure
suggests that MR microscopy would be well-suited for studies
of endolymphatic hydrops.},
Key = {fds132818}
}
@booklet{Henson94,
Author = {Henson, MM and Henson, OW and Gewalt, SL and Wilson, JL and Johnson,
GA},
Title = {Imaging the cochlea by magnetic resonance
microscopy.},
Journal = {Hear Res},
Volume = {75},
Number = {1-2},
Pages = {75-80},
Year = {1994},
Month = {May},
ISSN = {0378-5955},
url = {http://www.ncbi.nlm.nih.gov/pubmed/8071156},
Abstract = {The isolated, fixed cochlea of the mustached bat was studied
with three dimensional magnetic resonance (MR) microscopy.
The cochlea of this animal is about 4 mm in diameter and its
entire volume was imaged. With the field of view and matrix
size used, the volume elements (voxels) making up the volume
data set were isotropic 25 x 25 x 25 micron cubes. Three
dimensional (3D) MR microscopy based on isotropic voxels has
many advantages over commonly used light microscopy: 1) it
is non destructive; 2) it is much less time consuming; 3) no
dehydration is required and shrinkage is minimized; 4) the
data set can be used to create sections in any desired
plane; 5) the proper alignment of sections is inherent in
the 3D acquisition so that no reference points are required;
6) the entire data set can be viewed from any point of view
in a volume rendered image; 7) the data is digital and
features can be enhanced by computer image processing; and
8) the isotropic dimensions of the voxels make the data
well-suited for structural reconstructions and measurements.
Good images of the osseous spiral lamina, spiral ligament,
scala tympani, scala vestibuli, and nerve bundles were
obtained. The vestibular (Reissner's) membrane was easily
identified in the mustached bat and it appears to bulge into
the scala vestibuli. The visibility of this structure
suggests that MR microscopy would be well-suited for studies
of endolymphatic hydrops.},
Doi = {10.1016/0378-5955(94)90058-2},
Key = {Henson94}
}
@article{fds316054,
Author = {Wei, H and Xie, L and Dibb, R and Li, W and Decker, K and Zhang, Y and Johnson, GA and Liu, C},
Title = {Imaging whole-brain cytoarchitecture of mouse with MRI-based
quantitative susceptibility mapping.},
Journal = {Neuroimage},
Volume = {137},
Pages = {107-115},
Year = {2016},
Month = {August},
ISSN = {1053-8119},
url = {http://dx.doi.org/10.1016/j.neuroimage.2016.05.033},
Abstract = {The proper microstructural arrangement of complex neural
structures is essential for establishing the functional
circuitry of the brain. We present an MRI method to resolve
tissue microstructure and infer brain cytoarchitecture by
mapping the magnetic susceptibility in the brain at high
resolution. This is possible because of the heterogeneous
magnetic susceptibility created by varying concentrations of
lipids, proteins and irons from the cell membrane to
cytoplasm. We demonstrate magnetic susceptibility maps at a
nominal resolution of 10-μm isotropic, approaching the
average cell size of a mouse brain. The maps reveal many
detailed structures including the retina cell layers,
olfactory sensory neurons, barrel cortex, cortical layers,
axonal fibers in white and gray matter. Olfactory glomerulus
density is calculated and structural connectivity is traced
in the optic nerve, striatal neurons, and brainstem nerves.
The method is robust and can be readily applied on MRI
scanners at or above 7T.},
Doi = {10.1016/j.neuroimage.2016.05.033},
Key = {fds316054}
}
@article{fds174276,
Author = {AL Asirvatham and GA Johnson and EL Belden and EA Van Kirk and GE Moss and WJ Murdoch},
Title = {Immunization of mice against a synthetic N-terminal
extracellular domain gonadotropin-releasing hormone receptor
peptide: evidence for a direct uterine effect.},
Journal = {American journal of reproductive immunology (New York, N.Y.
: 1989)},
Volume = {32},
Number = {2},
Pages = {95-100},
Year = {1994},
Month = {September},
ISSN = {1046-7408},
Keywords = {Amino Acid Sequence • Animals • Female •
Immunization, Passive • Male • Mice • Mice,
Inbred BALB C • Molecular Sequence Data • Peptide
Fragments • Receptors, LHRH • Uterus •
Vaccination • chemistry • immunology •
immunology* • pathology},
Abstract = {PROBLEM: Immature male and female mice were immunized with a
synthetic peptide corresponding to amino acids 5-17
(ASLEQDPNHCSAI) of the mouse hypophyseal
gonadotropin-releasing hormone (GnRH) receptor. METHOD:
Effect of immunization (postpuberal) was restricted to the
uterus. Pituitary-gonadal functions were not altered.
RESULTS: The endometrial lining of immunized females was
thin and lacked glandular development. These observations
were corroborated in actively immunized and passively
immunized adult females. CONCLUSIONS: Apparently endometrial
cells express a unique surface antigen, though reactive with
antipeptide antibodies, that differs from the prototype
pituitary GnRH receptor. Antibodies that selectively inhibit
endometrial maturation might be used to treat proliferative
diseases of the uterus.},
Language = {eng},
Key = {fds174276}
}
@booklet{Asirvatham94,
Author = {A. L. Asirvatham and G. A. Johnson and E. L. Belden and E.
A. Vankirk and G. E. Moss and W. J. Murdoch},
Title = {Immunization of mice against a synthetic n-terminal
extracellular domain gonadotropin-releasing-hormone receptor
peptide - evidence for a direct uterine effect},
Journal = {American Journal Of Reproductive Immunology},
Volume = {32},
Number = {2},
Pages = {95 -- 100},
Year = {1994},
Month = {September},
Key = {Asirvatham94}
}
@booklet{Zelei90,
Author = {B. V. Zelei and C. J. Walker and G. A. Sawada and T. T.
Kawabe and K. A. Knight and A. E. Buhl and G. A. Johnson and A. R. Diani},
Title = {Immunohistochemical and autoradiographic findings suggest
that minoxidil is not localized in specific cells of
vibrissa, pelage, or scalp follicles},
Journal = {Cell And Tissue Research},
Volume = {262},
Number = {3},
Pages = {407 -- 413},
Year = {1990},
Month = {December},
Key = {Zelei90}
}
@article{fds174107,
Author = {BV Zelei and CJ Walker and GA Sawada and TT Kawabe and KA Knight and AE
Buhl, GA Johnson and AR Diani},
Title = {Immunohistochemical and autoradiographic findings suggest
that minoxidil is not localized in specific cells of
vibrissa, pelage, or scalp follicles.},
Journal = {Cell and tissue research},
Volume = {262},
Number = {3},
Pages = {407-13},
Year = {1990},
Month = {December},
ISSN = {0302-766X},
Keywords = {Animals • Autoradiography • Hair •
Immunohistochemistry • Male • Melanins •
Minoxidil • Rats • Scalp • Vibrissae •
cytology* • metabolism • metabolism*},
Abstract = {Immunohistochemistry with a minoxidil antibody suggested
that minoxidil-immunoreactivity is associated with the root
sheaths, laterally orientated differentiating matrix cells,
and dividing epithelial cells of cultured vibrissa follicles
of pigmented and albino neonatal mice. The dermal papilla
and connective tissue sheath were devoid of
minoxidil-immunoreactivity. To verify that
minoxodil-immunoreactivity in the follicles was specific,
immunostaining was conducted with dissected whisker pads,
formalin-fixed "dead" follicles, and sections of spleen,
liver and kidney (non-haired organs) cultured with
minoxidil. Microscopic examination revealed
minoxidil-immunoreactivity in all of these tissues.
Follicles and whisker pads cultured with minoxidil, then
washed for one h in media were devoid of
minoxidil-immunoreactivity. These data suggest that
minoxidil-immunoreactivity in cultured vibrissa follicles is
probably non-specific. Sections of skin from C3H and CF1
mice which were topically dosed with minoxidil (in vivo)
showed no minoxidil-immunoreactivity. Autoradiography
demonstrated that tritiated minoxidil was bound in vivo and
in vitro only to melanin granules in pigmented follicles of
rodent and human tissue. This is probably non-specific
binding since melanin is known to accumulate several
chemically and pharmacologically unrelated drugs. It is
reasonable to conclude that, under the conditions of these
experiments, minoxidil is not specifically localized in any
cells of whisker, pelage or, scalp follicles.},
Language = {eng},
Key = {fds174107}
}
@booklet{Harris86,
Author = {D. W. Harris and D. M. Sutter and G. A. Johnson and J. H.
Ludens},
Title = {Immunoreactive atrial natriuretic factors (ir-anf) in plasma
of conscious, unperturbed doca-salt hypertensive
rats},
Journal = {Clinical Research},
Volume = {34},
Number = {2},
Pages = {A479 -- A479},
Year = {1986},
Month = {April},
Key = {Harris86}
}
@article{fds180932,
Author = {G Wu and FW Bazer and RC Burghardt and GA Johnson and SW Kim and XL Li and MC
Satterfield, TE Spencer},
Title = {Impacts of amino acid nutrition on pregnancy outcome in
pigs: mechanisms and implications for swine
production.},
Journal = {Journal of animal science},
Volume = {88},
Number = {13 Suppl},
Pages = {E195-204},
Year = {2010},
Month = {April},
ISSN = {1525-3163},
url = {http://dx.doi.org/10.2527/jas.2009-2446},
Keywords = {Adipose Tissue • Amino Acids • Animal Nutritional
Physiological Phenomena • Animals • Diet •
Female • Fetal Development • Fetal Growth
Retardation • Litter Size • Muscle, Skeletal
• Placenta • Pregnancy • Pregnancy Outcome
• Swine • drug effects • embryology •
growth & development • metabolism • physiology
• physiology* • veterinary •
veterinary*},
Abstract = {Pigs suffer up to 50% embryonic and fetal loss during
gestation and exhibit the most severe naturally occurring
intrauterine growth retardation among livestock species.
Placental insufficiency is a major factor contributing to
suboptimal reproductive performance and reduced birth
weights of pigs. Enhancement of placental growth and
function through nutritional management offers an effective
solution to improving embryonic and fetal survival and
growth. We discovered an unusual abundance of the arginine
family of AA in porcine allantoic fluid (a reservoir of
nutrients) during early gestation, when placental growth is
most rapid. Arginine is metabolized to ornithine, proline,
and nitric oxide, and these compounds possess a plethora of
physiological functions. Nitric oxide is a vasodilator and
angiogenic factor, whereas both ornithine and proline are
substrates for placental synthesis of polyamines, which are
key regulators of protein synthesis and angiogenesis.
Additionally, arginine, leucine, glutamine, and proline
activate the mammalian target of rapamycin cell-signaling
pathway to enhance protein synthesis and cell proliferation
in placentae. To translate basic research on AA biochemistry
and nutrition into application, dietary supplementation with
0.83% l-arginine to gilts on d 14 to 28 or d 30 to 114 of
gestation increased the number and litter birth weight of
live-born piglets. In addition, supplementing the gestation
diet with 0.4% l-arginine plus 0.6% l-glutamine enhanced the
efficiency of nutrient utilization, reduced variation in
piglet birth weight, and increased litter birth weight. By
regulating syntheses of nitric oxide, polyamines, and
proteins, functional AA stimulate placental growth and the
transfer of nutrients from mother to embryo or fetus to
promote conceptus survival, growth, and development.},
Language = {eng},
Doi = {10.2527/jas.2009-2446},
Key = {fds180932}
}
@article{fds174076,
Author = {TE Spencer and GA Johnson and FW Bazer and RC Burghardt},
Title = {Implantation mechanisms: insights from the
sheep.},
Journal = {Reproduction (Cambridge, England)},
Volume = {128},
Number = {6},
Pages = {657-68},
Year = {2004},
Month = {December},
ISSN = {1470-1626},
url = {http://dx.doi.org/10.1530/rep.1.00398},
Keywords = {Animals • Blastocyst • Cell Adhesion Molecules
• Embryo Implantation • Embryonic Development
• Female • Gestational Age • Pregnancy •
Sheep • Uterus • physiology •
physiology*},
Abstract = {Implantation in all mammals involves shedding of the zona
pellucida, followed by orientation, apposition, attachment
and adhesion of the blastocyst to the endometrium.
Endometrial invasion does not occur in domestic ruminants;
thus, definitive implantation is achieved by adhesion of the
mononuclear trophoblast cells to the endometrial lumenal
epithelium (LE) and formation of syncytia by the fusion of
trophoblast binucleate cells with the LE. This review
highlights new information on mechanisms regulating the
implantation cascade in sheep. The embryo enters the uterus
on day 4 at the morula stage of development and then
develops into a blastocyst by day 6. The blastocyst sheds
the zona pellucida (day 8), elongates to a filamentous form
(days 11-16), and adheres to the endometrial LE (day 16).
Between days 14 and 16, the binucleate cells begin to
differentiate in the trophoblast and subsequently migrate
and fuse with the endometrial LE to form syncytia.
Continuous exposure of the endometrium to progesterone in
early pregnancy downregulates the progesterone receptors in
the epithelia, a process which is associated with loss of
the cell-surface mucin MUC1 and induction of several
secreted adhesion proteins. Recurrent early pregnancy loss
in the uterine gland knockout ewe model indicates that
secretions of the endometrial epithelia have a physiologic
role in blastocyst elongation and implantation. A number of
endometrial proteins have been identified as potential
regulators of blastocyst development and implantation in
sheep, including glycosylated cell adhesion molecule 1
(GlyCAM-1), galectin-15, integrins and osteopontin. The
epithelial derived secreted adhesion proteins (GlyCAM-1,
galectin-15 and osteopontin) are expressed in a dynamic
temporal and spatial manner and regulated by progesterone
and/or interferon tau, which is the pregnancy recognition
signal produced by the trophoblast during blastocyst
elongation. The noninvasive and protracted nature of
implantation in domestic animals provides valuable
opportunities to investigate fundamental processes of
implantation that are shared among all mammals.
Understanding of the cellular and molecular signals that
regulate uterine receptivity and implantation can be used to
diagnose and identify causes of recurrent pregnancy loss and
to improve pregnancy outcome in domestic animals and
humans.},
Language = {eng},
Doi = {10.1530/rep.1.00398},
Key = {fds174076}
}
@booklet{Farmer89,
Author = {T. H. R. Farmer and G. A. Johnson and G. P. Cofer and R. R.
Maronpot and D. Dixon and L. W. Hedlund},
Title = {Implanted coil mr microscopy of renal pathology},
Journal = {Magnetic Resonance In Medicine},
Volume = {10},
Number = {3},
Pages = {310 -- 323},
Year = {1989},
Month = {June},
Key = {Farmer89}
}
@article{fds269011,
Author = {Farmer, TH and Johnson, GA and Cofer, GP and Maronpot, RR and Dixon, D and Hedlund, LW},
Title = {Implanted coil MR microscopy of renal pathology.},
Journal = {Magn Reson Med},
Volume = {10},
Number = {3},
Pages = {310-323},
Year = {1989},
Month = {June},
ISSN = {0740-3194},
url = {http://www.ncbi.nlm.nih.gov/pubmed/2733588},
Keywords = {Animals • Electrodes, Implanted • Kidney •
Kidney Diseases • Magnetic Resonance Imaging •
Microscopy • Rats • Rats, Inbred Strains •
chemically induced • metabolism • methods* •
pathology*},
Abstract = {Inductively coupled implanted coils have been shown to
provide up to a 10-fold increase in signal-to-noise ratio
when compared to whole-body imaging of small animals. The
current study was designed to extend the implanted coil
imaging technique to a rodent model of renal pathology.
Resonant radiofrequency (RF) coils were implanted around the
left kidney of four rats and inductively coupled from within
a birdcage body coil. All images were acquired at 2 T using
a T1-weighted spin-echo sequence with TR = 500 ms and TE =
20 ms. In vivo MR microscopy with voxels of 117 x 117 x 2000
microns demonstrated cortex, inner and outer medulla, and
major vascular structures on baseline images. Mercuric
chloride-induced nephrotoxic acute tubular necrosis (ATN)
diminished cortico-medullary contrast at 24 h after dosing
with pathologic evaluation demonstrating nephrotoxic changes
in the inner cortex. The kidney regained a baseline MR
appearance 360 h after dosing and resolution of the damage
was confirmed with histology. T1 data were gathered on
excised kidneys as an adjunct to the images to help
correlate the loss and return of cortico-medullary contrast
with the pathology and pathophysiology of nephrotoxic ATN.
With implanted RF coils we were able to demonstrate renal
pathology and follow its subsequent resolution.
Specifically, loss and return of cortico-medullary contrast
as a result of nephrotoxic ATN were serially documented in
four rats. Such serial in vivo studies performed on single
animals should further the use of MR microscopy by
minimizing the number of animals required for adequate
biostatistics.},
Doi = {10.1002/mrm.1910100304},
Key = {fds269011}
}
@booklet{Mcadams87,
Author = {McAdams, HP and Johnson, GA and Suddarth, SA and Sherrier, RH and Ravin,
CE},
Title = {IMPLEMENTATION OF ADAPTIVE FILTRATION FOR DIGITAL CHEST
IMAGING.},
Journal = {Optical Engineering},
Volume = {26},
Number = {7},
Pages = {669-674},
Publisher = {SPIE-Intl Soc Optical Eng},
Year = {1987},
Month = {January},
url = {http://dx.doi.org/10.1117/12.7974133},
Abstract = {Previous work has demonstrated the potential for adaptive
filtration in processing digital chest images. The technique
uses the histogram of the image to determine the pixels (and
regions) in which edge enhancement is applied. This paper
extends that work by investigating the choice of parameters
used in selectively enhancing the mediastinum. The image is
separated into its low and high frequency components by
convolution with a square kernel. The effect of kernel size
was studied with a choice of 17 multiplied by 17 mm, which
was found to be sufficient to include the frequencies of
interest. A serious deficiency in previous implementations
of this technique is the existence of ringing artifacts at
the juncture of the lung and mediastinum. These result in
part from the use of a step function to specify the low
frequency image intensity above which high frequencies are
amplified. By replacing this step with a smoother (cosine)
function, the artifact can be removed.},
Doi = {10.1117/12.7974133},
Key = {Mcadams87}
}
@booklet{Zhang03,
Author = {X. W. Zhang and T. M. Yelbuz and G. P. Cofer and M. A. Choma and M. L. Kirby and G. A. Johnson},
Title = {Improved preparation of chick embryonic samples for magnetic
resonance microscopy},
Journal = {Magnetic Resonance In Medicine},
Volume = {49},
Number = {6},
Pages = {1192 -- 1195},
Year = {2003},
Month = {June},
Key = {Zhang03}
}
@article{fds269008,
Author = {Zhang, X and Yelbuz, TM and Cofer, GP and Choma, MA and Kirby, ML and Johnson, GA},
Title = {Improved preparation of chick embryonic samples for magnetic
resonance microscopy.},
Journal = {Magn Reson Med},
Volume = {49},
Number = {6},
Pages = {1192-1195},
Year = {2003},
Month = {June},
ISSN = {0740-3194},
url = {http://www.ncbi.nlm.nih.gov/pubmed/12768599},
Keywords = {Animals • Chick Embryo • Contrast Media •
Heart • Image Enhancement* • Magnetic Resonance
Imaging* • Microscopy • anatomy & histology •
embryology* • instrumentation*},
Abstract = {Previous work demonstrated the power of three-dimensional
(3D) magnetic resonance microscopy (MRM) to follow
complicated morphologic development in the embryonic
cardiovascular system. In this study we describe a new
dual-contrast method for specimen preparation that combines
perfusion fixation and immersion in fixative with macro- and
small molecular gadolinium agents to provide enhanced
definition of both the heart wall and chamber. MRM was
performed at 9.4 T with image resolutions of 25, 31, and 50
microm isotropic voxels for three stages of chick embryos
(day 4, day 5.5, and day 9), and compared to histological
sections of the same embryos. The results show considerable
improvement of image quality over previous efforts, with
better signal-to-noise ratio (SNR) and contrast between the
cardiac chamber and myocardial wall. Excellent correlation
was shown between the MRM images and histological sections.
Thus, 3D high-resolution MRM in combination with the
dual-contrast technique is useful for acquiring quantitative
3D morphologic data regarding heart development.},
Doi = {10.1002/mrm.10460},
Key = {fds269008}
}
@article{fds268822,
Author = {Brinegar, C and Schmitter, SS and Mistry, NN and Johnson, GA and Liang,
Z-P},
Title = {Improving temporal resolution of pulmonary perfusion imaging
in rats using the partially separable functions
model.},
Journal = {Magn Reson Med},
Volume = {64},
Number = {4},
Pages = {1162-1170},
Year = {2010},
Month = {October},
ISSN = {1522-2594},
url = {http://www.ncbi.nlm.nih.gov/pubmed/20564601},
Keywords = {Algorithms* • Animals • Blood Flow Velocity •
Computer Simulation • Female • Image Enhancement
• Image Interpretation, Computer-Assisted •
Magnetic Resonance Angiography • Models,
Cardiovascular* • Pulmonary Artery • Pulmonary
Circulation • Rats • Rats, Inbred F344 •
Reproducibility of Results • Sensitivity and
Specificity • anatomy & histology • methods •
methods* • physiology • physiology*},
Abstract = {Dynamic contrast-enhanced MRI (or DCE-MRI) is a useful tool
for measuring blood flow and perfusion, and it has found use
in the study of pulmonary perfusion in animal models.
However, DCE-MRI experiments are difficult in small animals
such as rats. A recently developed method known as
Interleaved Radial Imaging and Sliding window-keyhole (IRIS)
addresses this problem by using a data acquisition scheme
that covers (k,t)-space with data acquired from multiple
bolus injections of a contrast agent. However, the temporal
resolution of IRIS is limited by the effects of temporal
averaging inherent in the sliding window and keyhole
operations. This article describes a new method to cover
(k,t)-space based on the theory of partially separable
functions (PSF). Specifically, a sparse sampling of
(k,t)-space is performed to acquire two data sets, one with
high-temporal resolution and the other with extended k-space
coverage. The high-temporal resolution training data are
used to determine the temporal basis functions of the PSF
model, whereas the other data set is used to determine the
spatial variations of the model. The proposed method was
validated by simulations and demonstrated by an experimental
study. In this particular study, the proposed method
achieved a temporal resolution of 32 msec.},
Language = {eng},
Doi = {10.1002/mrm.22500},
Key = {fds268822}
}
@booklet{Cserhati89,
Author = {A. F. Cserhati and V. K. Lucian and E. K. Austin and G. A.
Johnson and P. D. Sarmiento and G. A. West and C.
Magee},
Title = {Impurity content, redistribution and interface topography in
poly and amorphous-silicon based cobalt polycides},
Journal = {Applied Surface Science},
Volume = {38},
Number = {1-4},
Pages = {195 -- 195},
Year = {1989},
Month = {September},
Key = {Cserhati89}
}
@article{fds132776,
Author = {MD Hollett and GP Cofer and GA Johnson},
Title = {In situ magnetic resonance microscopy.},
Journal = {Investigative radiology, UNITED STATES},
Volume = {22},
Number = {12},
Pages = {965-8},
Year = {1987},
Month = {December},
ISSN = {0020-9996},
Keywords = {Animals • Kidney • Magnetic Resonance Imaging
• Models, Structural • Rats • anatomy &
histology* • methods*},
Abstract = {Recent developments in MR permit imaging at microscopic
resolution. Efforts have focused on small samples that fit
entirely in the imaging probe. Extension of the techniques
to imaging of individual organs in small animals is
complicated by both the need to acquire an excessive number
of phase encodings and limited signal to noise. Implantable
radiofrequency coils described in this work eliminate both
problems, permitting MR microscopy in the kidney of a live
200-g rat with spatial resolution of 117 X 117 X 1250 mu
(.02 mm3). Inductive coupling permits complete freedom from
external leads. A phantom designed to evaluate dielectric
losses is described. Both phantom and in vivo comparison of
live kidney images demonstrate the tenfold improvement in
signal to noise obtained with the implantable
coil.},
Key = {fds132776}
}
@booklet{Hollett87,
Author = {Hollett, MD and Cofer, GP and Johnson, GA},
Title = {In situ magnetic resonance microscopy.},
Journal = {Invest Radiol},
Volume = {22},
Number = {12},
Pages = {965-968},
Year = {1987},
Month = {December},
ISSN = {0020-9996},
url = {http://www.ncbi.nlm.nih.gov/pubmed/3440731},
Abstract = {Recent developments in MR permit imaging at microscopic
resolution. Efforts have focused on small samples that fit
entirely in the imaging probe. Extension of the techniques
to imaging of individual organs in small animals is
complicated by both the need to acquire an excessive number
of phase encodings and limited signal to noise. Implantable
radiofrequency coils described in this work eliminate both
problems, permitting MR microscopy in the kidney of a live
200-g rat with spatial resolution of 117 X 117 X 1250 mu
(.02 mm3). Inductive coupling permits complete freedom from
external leads. A phantom designed to evaluate dielectric
losses is described. Both phantom and in vivo comparison of
live kidney images demonstrate the tenfold improvement in
signal to noise obtained with the implantable
coil.},
Doi = {10.1097/00004424-198712000-00009},
Key = {Hollett87}
}
@article{fds174140,
Author = {CA Matyac and GP Cofer and JE Bailey and GA Johnson},
Title = {In Situ Observations of Root-gall Formation Using Nuclear
Magnetic Resonance Imaging.},
Journal = {Journal of nematology},
Volume = {21},
Number = {1},
Pages = {131-4},
Year = {1989},
Month = {January},
ISSN = {0022-300X},
Language = {eng},
Key = {fds174140}
}
@booklet{Matyac89,
Author = {Matyac, CA and Cofer, GP and Bailey, JE and Johnson,
GA},
Title = {In Situ Observations of Root-gall Formation Using Nuclear
Magnetic Resonance Imaging.},
Journal = {J Nematol},
Volume = {21},
Number = {1},
Pages = {131-134},
Year = {1989},
Month = {January},
ISSN = {0022-300X},
url = {http://www.ncbi.nlm.nih.gov/pubmed/19287587},
Key = {Matyac89}
}
@article{fds132788,
Author = {CT Huesgen and PC Burger and BJ Crain and GA Johnson},
Title = {In vitro MR microscopy of the hippocampus in Alzheimer's
disease.},
Journal = {Neurology, UNITED STATES},
Volume = {43},
Number = {1},
Pages = {145-52},
Year = {1993},
Month = {January},
ISSN = {0028-3878},
Keywords = {Aged • Aged, 80 and over • Alzheimer Disease
• Fourier Analysis • Hippocampus • Humans
• Magnetic Resonance Imaging* • Middle Aged •
Neurofibrillary Tangles • pathology •
pathology*},
Abstract = {We used MR microscopy at 7 tesla to identify the anatomy of
the degenerating hippocampus in Alzheimer's disease (AD),
which we then correlated with the histopathologic findings
in the same specimens. The specimens studied were resected
postmortem from 13 patients with confirmed AD and from nine
age-matched controls. We imaged the specimens in the coronal
plane using either three-dimensional Fourier encoding or
single-slice Carr, Purcell, Meiboom, Gill (CPMG) spin echo
sequences. On all specimens imaged with the CPMG pulse
sequence, we calculated the T2 relaxation times for
subfields within the hippocampus. Histologic sections were
taken from each specimen and compared with the corresponding
MR image. Using histologic boundaries, we quantified the
number of neuritic plaques and neurofibrillary tangles in
each hippocampal subfield. We measured the area,
morphometric characteristics, and width of identifiable
signal variant regions on each image and compared these
measurements with the histopathologic findings. The mean
cross-sectional area of the hippocampus in AD was decreased
by 31% compared with the control group. This atrophy was
highly correlated with tangle counts within the hippocampus,
but not with plaque counts. The width of the gray matter in
hippocampal area CA1, as identified by MR, correlated with
the total area of the hippocampus. An age-related decrease
in the size of a low-signal region that corresponds
histologically to input projections comprising part of the
perforant pathway was identified. Measurements of the T2
relaxation times of hippocampal subfields showed little
regional variability and were not accurate indicators of
disease presence or severity (p > 0.05).},
Key = {fds132788}
}
@booklet{Huesgen93,
Author = {Huesgen, CT and Burger, PC and Crain, BJ and Johnson,
GA},
Title = {In vitro MR microscopy of the hippocampus in Alzheimer's
disease.},
Journal = {Neurology},
Volume = {43},
Number = {1},
Pages = {145-152},
Year = {1993},
Month = {January},
ISSN = {0028-3878},
url = {http://www.ncbi.nlm.nih.gov/pubmed/8423879},
Abstract = {We used MR microscopy at 7 tesla to identify the anatomy of
the degenerating hippocampus in Alzheimer's disease (AD),
which we then correlated with the histopathologic findings
in the same specimens. The specimens studied were resected
postmortem from 13 patients with confirmed AD and from nine
age-matched controls. We imaged the specimens in the coronal
plane using either three-dimensional Fourier encoding or
single-slice Carr, Purcell, Meiboom, Gill (CPMG) spin echo
sequences. On all specimens imaged with the CPMG pulse
sequence, we calculated the T2 relaxation times for
subfields within the hippocampus. Histologic sections were
taken from each specimen and compared with the corresponding
MR image. Using histologic boundaries, we quantified the
number of neuritic plaques and neurofibrillary tangles in
each hippocampal subfield. We measured the area,
morphometric characteristics, and width of identifiable
signal variant regions on each image and compared these
measurements with the histopathologic findings. The mean
cross-sectional area of the hippocampus in AD was decreased
by 31% compared with the control group. This atrophy was
highly correlated with tangle counts within the hippocampus,
but not with plaque counts. The width of the gray matter in
hippocampal area CA1, as identified by MR, correlated with
the total area of the hippocampus. An age-related decrease
in the size of a low-signal region that corresponds
histologically to input projections comprising part of the
perforant pathway was identified. Measurements of the T2
relaxation times of hippocampal subfields showed little
regional variability and were not accurate indicators of
disease presence or severity (p > 0.05).},
Doi = {10.1212/wnl.43.1_part_1.145},
Key = {Huesgen93}
}
@booklet{Benveniste99a,
Author = {Benveniste, H and Qui, H and Hedlund, LW and Hüttemeier, PC and Steele,
SM and Johnson, GA},
Title = {In vivo diffusion-weighted magnetic resonance microscopy of
rat spinal cord: effect of ischemia and intrathecal
hyperbaric 5% lidocaine.},
Journal = {Reg Anesth Pain Med},
Volume = {24},
Number = {4},
Pages = {311-318},
Year = {1999},
ISSN = {1098-7339},
url = {http://www.ncbi.nlm.nih.gov/pubmed/10445769},
Abstract = {BACKGROUND AND OBJECTIVES: Pathophysiologic mechanisms
underlying persistent neurologic deficits after continuous
spinal anesthesia using hyperbaric 5% lidocaine are still
not well understood. It has been suggested that high-dose
intrathecal lidocaine induces irreversible conduction block
and even ischemia in white matter tracts by breakdown of the
blood-nerve barrier. In this study, we use
diffusion-weighted magnetic resonance microscopy to
characterize the effect of intrathecal hyperbaric 5%
lidocaine in rat spinal cord. The parameter measured with
DWM, is an "apparent diffusion coefficient," (ADC), which
can be used to exclude the presence of ischemia. METHODS:
Female Fischer CDF rats were used. Group 1 (n = 5) was
exposed to ischemia, group 2 (n = 7) was exposed to
intrathecal 5% hyperbaric lidocaine, and group 3 (n = 5) was
exposed to intrathecal 7.5% glucose. Diffusion-weighted MR
images in group 1 were acquired before and after ischemia
induced by cardiac arrest and in groups 2 and 3 rats prior
to and during perfusion of the spinal catheter with either
5% hyperbaric lidocaine or 7.5% glucose. RESULTS: Ischemia
decreased the ADC by 40% in gray matter and by 30% in white
matter of spinal cord. Continuous intrathecal anesthesia
with hyperbaric 5% lidocaine did not affect the spinal cord
ADC. Further, 7.5% intrathecal glucose had no effect on ADCs
in gray or white matter of spinal cord. CONCLUSIONS:
Ischemia reduced the ADC in both spinal cord white and gray
matter. Hyperbaric 5% lidocaine did not affect the spinal
cord ADC during the first 1.5 hours. We suggest that 5%
hyperbaric lidocaine does not induce irreversible neurologic
deficits by causing spinal cord ischemia.},
Doi = {10.1016/s1098-7339(99)90104-9},
Key = {Benveniste99a}
}
@article{fds132840,
Author = {H Benveniste and H Qui and LW Hedlund and PC Hüttemeier and SM Steele and GA Johnson},
Title = {In vivo diffusion-weighted magnetic resonance microscopy of
rat spinal cord: effect of ischemia and intrathecal
hyperbaric 5% lidocaine.},
Journal = {Regional anesthesia and pain medicine, UNITED
STATES},
Volume = {24},
Number = {4},
Pages = {311-8},
ISSN = {1098-7339},
Keywords = {Anesthetics, Local • Animals • Blood Pressure
• Catheterization • Dose-Response Relationship,
Drug • Female • Heart Rate • Injections,
Spinal • Ischemia • Lidocaine • Magnetic
Resonance Imaging • Microscopy • Rats • Rats,
Inbred F344 • Spinal Cord • anatomy & histology*
• blood supply* • chemically induced* •
cytology • diagnosis* • drug effects •
methods • pathology • physiopathology •
toxicity*},
Abstract = {BACKGROUND AND OBJECTIVES: Pathophysiologic mechanisms
underlying persistent neurologic deficits after continuous
spinal anesthesia using hyperbaric 5% lidocaine are still
not well understood. It has been suggested that high-dose
intrathecal lidocaine induces irreversible conduction block
and even ischemia in white matter tracts by breakdown of the
blood-nerve barrier. In this study, we use
diffusion-weighted magnetic resonance microscopy to
characterize the effect of intrathecal hyperbaric 5%
lidocaine in rat spinal cord. The parameter measured with
DWM, is an "apparent diffusion coefficient," (ADC), which
can be used to exclude the presence of ischemia. METHODS:
Female Fischer CDF rats were used. Group 1 (n = 5) was
exposed to ischemia, group 2 (n = 7) was exposed to
intrathecal 5% hyperbaric lidocaine, and group 3 (n = 5) was
exposed to intrathecal 7.5% glucose. Diffusion-weighted MR
images in group 1 were acquired before and after ischemia
induced by cardiac arrest and in groups 2 and 3 rats prior
to and during perfusion of the spinal catheter with either
5% hyperbaric lidocaine or 7.5% glucose. RESULTS: Ischemia
decreased the ADC by 40% in gray matter and by 30% in white
matter of spinal cord. Continuous intrathecal anesthesia
with hyperbaric 5% lidocaine did not affect the spinal cord
ADC. Further, 7.5% intrathecal glucose had no effect on ADCs
in gray or white matter of spinal cord. CONCLUSIONS:
Ischemia reduced the ADC in both spinal cord white and gray
matter. Hyperbaric 5% lidocaine did not affect the spinal
cord ADC during the first 1.5 hours. We suggest that 5%
hyperbaric lidocaine does not induce irreversible neurologic
deficits by causing spinal cord ischemia.},
Key = {fds132840}
}
@article{fds132806,
Author = {RD Black and HL Middleton and GD Cates and GP Cofer and B Driehuys and W
Happer, LW Hedlund and GA Johnson, MD Shattuck and JC
Swartz},
Title = {In vivo He-3 MR images of guinea pig lungs.},
Journal = {Radiology, UNITED STATES},
Volume = {199},
Number = {3},
Pages = {867-70},
Year = {1996},
Month = {June},
ISSN = {0033-8419},
Keywords = {Animals • Guinea Pigs • Helium • Lung •
Magnetic Resonance Imaging • Male • Radioisotopes
• anatomy & histology* • instrumentation •
methods* • statistics & numerical data},
Abstract = {The authors imaged the lungs of live guinea pigs with
hyperpolarized (HP) helium-3 as a magnetic resonance (MR)
signal source. HP He-3 gas produced through spin exchange
with rubidium metal vapor was delivered through an
MR-compatible, small-animal ventilator. Two- and
three-dimensional lung images acquired with
ventilation-gated, radial k-space sampling showed complete
ventilation of both lungs. All images were of high quality,
demonstrating that HP He-3 allows high-signal-intensity MR
imaging in living systems.},
Key = {fds132806}
}
@booklet{Black96,
Author = {Black, RD and Middleton, HL and Cates, GD and Cofer, GP and Driehuys, B and Happer, W and Hedlund, LW and Johnson, GA and Shattuck, MD and Swartz,
JC},
Title = {In vivo He-3 MR images of guinea pig lungs.},
Journal = {Radiology},
Volume = {199},
Number = {3},
Pages = {867-870},
Year = {1996},
Month = {June},
ISSN = {0033-8419},
url = {http://www.ncbi.nlm.nih.gov/pubmed/8638019},
Abstract = {The authors imaged the lungs of live guinea pigs with
hyperpolarized (HP) helium-3 as a magnetic resonance (MR)
signal source. HP He-3 gas produced through spin exchange
with rubidium metal vapor was delivered through an
MR-compatible, small-animal ventilator. Two- and
three-dimensional lung images acquired with
ventilation-gated, radial k-space sampling showed complete
ventilation of both lungs. All images were of high quality,
demonstrating that HP He-3 allows high-signal-intensity MR
imaging in living systems.},
Doi = {10.1148/radiology.199.3.8638019},
Key = {Black96}
}
@article{fds204265,
Author = {CT Badea and LW Hedlund and Y Qi and B Berridge and GA
Johnson},
Title = {In vivo imaging of rat coronary arteries using bi-plane
digital subtraction angiography.},
Journal = {Journal of pharmacological and toxicological
methods},
Volume = {64},
Number = {2},
Pages = {151-7},
Year = {2011},
Month = {June},
ISSN = {1873-488X},
url = {http://dx.doi.org/10.1016/j.vascn.2011.05.008},
Keywords = {Angiography, Digital Subtraction • Animals •
Contrast Media • Coronary Angiography • Coronary
Circulation • Male • Myocardial Perfusion Imaging
• Nitroprusside • Rats • Rats, Sprague-Dawley
• Time Factors • Vasodilator Agents •
administration & dosage • drug effects* •
instrumentation • methods* • pharmacology},
Abstract = {BACKGROUND: X-ray based digital subtraction angiography
(DSA) is a common clinical imaging method for vascular
morphology and function. Coronary artery characterization is
one of its most important applications. We show that
bi-plane DSA of rat coronary arteries can provide a powerful
imaging tool for translational safety assessment in drug
discovery. METHODS: A novel, dual tube/detector system,
constructed explicitly for preclinical imaging, supports
image acquisition at 10 frames/s with 88-micron spatial
resolution. Ventilation, x-ray exposure, and contrast
injection are all precisely synchronized using a biological
sequence controller implemented as a LabVIEW application. A
set of experiments were performed to test and optimize the
sampling and image quality. We applied the DSA imaging
protocol to record changes in the visualization of
coronaries and myocardial perfusion induced by a vasodilator
drug, nitroprusside. The drug was infused into a tail vein
catheter using a peristaltic infusion pump at a rate of 0.07
mL/h for 3 min (dose: 0.0875 mg). Multiple DSA sequences
were acquired before, during, and up to 25 min after drug
infusion. Perfusion maps of the heart were generated in
MATLAB to compare the drug effects over time. RESULTS: The
best trade-off between the injection time, pressure, and
image quality was achieved at 60 PSI, with the injection of
150 ms occurring early in diastole (60 ms delay) and
resulting in the delivery of 113 μL of contrast agent. DSA
images clearly show the main branches of the coronary
arteries in an intact, beating heart. The drug test
demonstrated that DSA can detect relative changes in
coronary circulation via perfusion maps. CONCLUSIONS: The
methodology for DSA imaging of rat coronary arteries can
serve as a template for future translational studies to
assist in safety evaluation of new pharmaceuticals. Although
x-ray imaging involves radiation, the associated dose (0.4
Gy) is not a major limitation.},
Language = {eng},
Doi = {10.1016/j.vascn.2011.05.008},
Key = {fds204265}
}
@article{fds268811,
Author = {Badea, CT and Hedlund, LW and Qi, Y and Berridge, B and Johnson,
GA},
Title = {In vivo imaging of rat coronary arteries using bi-plane
digital subtraction angiography.},
Journal = {J Pharmacol Toxicol Methods},
Volume = {64},
Number = {2},
Pages = {151-157},
Year = {2011},
url = {http://www.ncbi.nlm.nih.gov/pubmed/21683146},
Abstract = {INTRODUCTION: X-ray based digital subtraction angiography
(DSA) is a common clinical imaging method for vascular
morphology and function. Coronary artery characterization is
one of its most important applications. We show that
bi-plane DSA of rat coronary arteries can provide a powerful
imaging tool for translational safety assessment in drug
discovery. METHODS: A novel, dual tube/detector system,
constructed explicitly for preclinical imaging, supports
image acquisition at 10 frames/s with 88-micron spatial
resolution. Ventilation, x-ray exposure, and contrast
injection are all precisely synchronized using a biological
sequence controller implemented as a LabVIEW application. A
set of experiments were performed to test and optimize the
sampling and image quality. We applied the DSA imaging
protocol to record changes in the visualization of
coronaries and myocardial perfusion induced by a vasodilator
drug, nitroprusside. The drug was infused into a tail vein
catheter using a peristaltic infusion pump at a rate of 0.07
mL/h for 3 min (dose: 0.0875 mg). Multiple DSA sequences
were acquired before, during, and up to 25 min after drug
infusion. Perfusion maps of the heart were generated in
MATLAB to compare the drug effects over time. RESULTS: The
best trade-off between the injection time, pressure, and
image quality was achieved at 60 PSI, with the injection of
150 ms occurring early in diastole (60 ms delay) and
resulting in the delivery of 113 μL of contrast agent. DSA
images clearly show the main branches of the coronary
arteries in an intact, beating heart. The drug test
demonstrated that DSA can detect relative changes in
coronary circulation via perfusion maps. CONCLUSIONS: The
methodology for DSA imaging of rat coronary arteries can
serve as a template for future translational studies to
assist in safety evaluation of new pharmaceuticals. Although
x-ray imaging involves radiation, the associated dose (0.4
Gy) is not a major limitation.},
Doi = {10.1016/j.vascn.2011.05.008},
Key = {fds268811}
}
@article{fds174200,
Author = {GA Johnson and SJ Boukma and EG Kim},
Title = {In vivo inhibition of dopamine beta-hydroxylase by
1-phenyl-3-(2-thiazolyl)-2-thiourea (U-14,624).},
Journal = {The Journal of pharmacology and experimental
therapeutics},
Volume = {171},
Number = {1},
Pages = {80-7},
Year = {1970},
Month = {January},
ISSN = {0022-3565},
Keywords = {Adrenal Glands • Adrenalectomy • Animals •
Brain • Brain Chemistry • Carbon Isotopes •
Catecholamines • Disulfiram • Dopamine •
Dopamine Antagonists* • Hydrolases • Male •
Metaraminol • Mice • Monoamine Oxidase Inhibitors
• Myocardium • Norepinephrine • Rats •
Species Specificity • Thiazoles • Thiourea •
Time Factors • Tritium • Tyrosine • analysis
• antagonists & inhibitors* • biosynthesis •
drug effects • enzymology • metabolism •
metabolism* • pharmacology • pharmacology* •
physiology},
Language = {eng},
Key = {fds174200}
}
@booklet{Veres91a,
Author = {Veres, JS and Johnson, GA and Kramer, PJ},
Title = {In vivo Magnetic Resonance Imaging of Blechnum Ferns:
Changes in T1 and N (H) During Dehydration and
Rehydration},
Journal = {American Journal of Botany},
Volume = {78},
Number = {1},
Pages = {80-80},
Publisher = {Wiley},
Year = {1991},
Month = {January},
ISSN = {0002-9122},
url = {http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:A1991EW16700009&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=47d3190e77e5a3a53558812f597b0b92},
Doi = {10.2307/2445231},
Key = {Veres91a}
}
@article{fds132893,
Author = {M Brouwer and DW Engel and J Bonaventura and GA Johnson},
Title = {In vivo magnetic resonance imaging of the blue crab,
Callinectes sapidus: effect of cadmium accumulation in
tissues on proton relaxation properties.},
Journal = {The Journal of experimental zoology, UNITED
STATES},
Volume = {263},
Number = {1},
Pages = {32-40},
Year = {1992},
Month = {August},
ISSN = {0022-104X},
Keywords = {Animals • Brachyura • Cadmium • Copper •
Diet • Liver • Magnetic Resonance Imaging* •
Male • Organ Specificity • Pancreas • Trace
Elements • administration & dosage • analysis
• anatomy & histology • anatomy & histology*
• metabolism},
Abstract = {Nuclear magnetic resonance imaging (MRI) has been used to
visualize the internal anatomy of a living blue crab. The
resolution obtained in these studies was sufficient to
distinguish individual organs by the differences in their
proton densities and proton relaxation properties. T1
(spin-lattice relaxation time)-weighted imaging revealed the
lipid-rich nature of the hepatopancreas and gonadal tissue.
To evaluate the effect of metal-induced stress on the
different organs, crabs were exposed to elevated levels of
cadmium in their diet, which resulted in increased
concentrations of both cadmium and copper in the
hepatopancreas. The spin-spin relaxation time, T2, of mobile
protons in the metal-exposed tissue was significantly
greater than T2 in the control tissues. These measurements
suggest that the excess copper in the exposed tissues was
diamagnetic [Cu(I)], since the presence of paramagnetic
copper [Cu(II)] would result in a decrease of observed T2
values. We hypothesize that the increased T2 value is a
reflection of increased free water in the hepatopancreas.
These studies show that magnetic resonance imaging is an
important nondestructive tool for the study of morphological
and physiological changes that occur in marine invertebrates
in response to anthropogenic and natural
stresses.},
Key = {fds132893}
}
@booklet{Brouwer92,
Author = {Brouwer, M and Engel, DW and Bonaventura, J and Johnson,
GA},
Title = {In vivo magnetic resonance imaging of the blue crab,
Callinectes sapidus: effect of cadmium accumulation in
tissues on proton relaxation properties.},
Journal = {J Exp Zool},
Volume = {263},
Number = {1},
Pages = {32-40},
Year = {1992},
Month = {August},
ISSN = {0022-104X},
url = {http://www.ncbi.nlm.nih.gov/pubmed/1645119},
Abstract = {Nuclear magnetic resonance imaging (MRI) has been used to
visualize the internal anatomy of a living blue crab. The
resolution obtained in these studies was sufficient to
distinguish individual organs by the differences in their
proton densities and proton relaxation properties. T1
(spin-lattice relaxation time)-weighted imaging revealed the
lipid-rich nature of the hepatopancreas and gonadal tissue.
To evaluate the effect of metal-induced stress on the
different organs, crabs were exposed to elevated levels of
cadmium in their diet, which resulted in increased
concentrations of both cadmium and copper in the
hepatopancreas. The spin-spin relaxation time, T2, of mobile
protons in the metal-exposed tissue was significantly
greater than T2 in the control tissues. These measurements
suggest that the excess copper in the exposed tissues was
diamagnetic [Cu(I)], since the presence of paramagnetic
copper [Cu(II)] would result in a decrease of observed T2
values. We hypothesize that the increased T2 value is a
reflection of increased free water in the hepatopancreas.
These studies show that magnetic resonance imaging is an
important nondestructive tool for the study of morphological
and physiological changes that occur in marine invertebrates
in response to anthropogenic and natural
stresses.},
Doi = {10.1002/jez.1402630105},
Key = {Brouwer92}
}
@booklet{Cofer89,
Author = {Cofer, GP and Brown, JM and Allan Johnson and G},
Title = {In vivo magnetic resonance microscopy at 5
μm},
Journal = {Journal of Magnetic Resonance (1969)},
Volume = {83},
Number = {3},
Pages = {608-616},
Publisher = {Elsevier BV},
Year = {1989},
Month = {January},
ISSN = {0022-2364},
url = {http://dx.doi.org/10.1016/0022-2364(89)90354-5},
Doi = {10.1016/0022-2364(89)90354-5},
Key = {Cofer89}
}
@article{fds157096,
Author = {JM Brown and GA Johnson and PJ Kramer},
Title = {In Vivo Magnetic Resonance Microscopy of Changing Water
Content in Pelargonium hortorum Roots.},
Journal = {Plant physiology},
Volume = {82},
Number = {4},
Pages = {1158-1160},
Year = {1986},
Month = {December},
ISSN = {0032-0889},
Abstract = {Magnetic resonance imaging (MRI) was used to
nondestructively observe changes in water content in roots
of Pelargonium hortorum x Bailey during a period of
relatively rapid transpiration. Anatomical regions of the
root could be differentiated with a spatial resolution of
0.1 x 0.1 mm. MRI shows great potential for study of
plant-water relations.},
Key = {fds157096}
}
@booklet{Brown86,
Author = {Brown, JM and Johnson, GA and Kramer, PJ},
Title = {In Vivo Magnetic Resonance Microscopy of Changing Water
Content in Pelargonium hortorum Roots.},
Journal = {Plant Physiol},
Volume = {82},
Number = {4},
Pages = {1158-1160},
Year = {1986},
Month = {December},
ISSN = {0032-0889},
url = {http://www.ncbi.nlm.nih.gov/pubmed/16665154},
Abstract = {Magnetic resonance imaging (MRI) was used to
nondestructively observe changes in water content in roots
of Pelargonium hortorum x Bailey during a period of
relatively rapid transpiration. Anatomical regions of the
root could be differentiated with a spatial resolution of
0.1 x 0.1 mm. MRI shows great potential for study of
plant-water relations.},
Doi = {10.1104/pp.82.4.1158},
Key = {Brown86}
}
@article{fds269086,
Author = {Chawla, MS and Chen, XJ and Möller, HE and Cofer, GP and Wheeler, CT and Hedlund, LW and Johnson, GA},
Title = {In vivo magnetic resonance vascular imaging using
laser-polarized 3He microbubbles.},
Journal = {Proc Natl Acad Sci U S A},
Volume = {95},
Number = {18},
Pages = {10832-10835},
Year = {1998},
Month = {September},
ISSN = {0027-8424},
url = {http://www.ncbi.nlm.nih.gov/pubmed/9724790},
Keywords = {Angiography • Animals • Helium • Lasers
• Magnetic Resonance Imaging • Male • Rats
• Rats, Sprague-Dawley • methods*},
Abstract = {Laser-polarized gases (3He and 129Xe) are currently being
used in magnetic resonance imaging as strong signal sources
that can be safely introduced into the lung. Recently,
researchers have been investigating other tissues using
129Xe. These studies use xenon dissolved in a carrier such
as lipid vesicles or blood. Since helium is much less
soluble than xenon in these materials, 3He has been used
exclusively for imaging air spaces. However, considering
that the signal of 3He is more than 10 times greater than
that of 129Xe for presently attainable polarization levels,
this work has focused on generating a method to introduce
3He into the vascular system. We addressed the low
solubility issue by producing suspensions of 3He
microbubbles. Here, we provide the first vascular images
obtained with laser-polarized 3He. The potential increase in
signal and absence of background should allow this technique
to produce high-resolution angiographic images. In addition,
quantitative measurements of blood flow velocity and tissue
perfusion will be feasible.},
Doi = {10.1073/pnas.95.18.10832},
Key = {fds269086}
}
@booklet{Chawla98,
Author = {M. S. Chawla and X. J. Chen and H. E. Moller and G. P. Cofer and C. T. Wheeler and L. W. Hedlund and G. A.
Johnson},
Title = {In vivo magnetic resonance vascular imaging using
laser-polarized He-3 microbubbles},
Journal = {Proceedings Of The National Academy Of Sciences Of The
United States Of America},
Volume = {95},
Number = {18},
Pages = {10832 -- 10835},
Year = {1998},
Month = {September},
Key = {Chawla98}
}
@article{fds132790,
Author = {GA Johnson and JH Maki},
Title = {In vivo measurement of proton diffusion in the presence of
coherent motion.},
Journal = {Investigative radiology, UNITED STATES},
Volume = {26},
Number = {6},
Pages = {540-5},
Year = {1991},
Month = {June},
ISSN = {0020-9996},
Keywords = {Acetone • Animals • Diffusion • Dimethyl
Sulfoxide • Magnetic Resonance Imaging • Movement
• Protons* • Rats • Water •
methods*},
Abstract = {Measurement of the self-diffusion coefficient D of water in
tissue has been performed traditionally using the technique
proposed by Stejskal and Tanner. A variant of that technique
is shown here, employing flow-compensated gradients that
significantly reduce the sensitivity to small coherent
motions that are common in body imaging. An interleaved
sequence with four values of diffusion-sensitizing gradient
(b) minimizes registration errors. Eddy currents and other
systematic errors are reduced, permitting the measurement of
standards in an imaging context within 5% of nonimaging
values in the literature. The flow-compensated sequence
permits the measure of D for tissues in the abdominal cavity
of the rat. We present in vivo measurements of D for the
following rat tissues; liver, kidney (cortex), kidney
(medulla) muscle, brain, fat.},
Key = {fds132790}
}
@booklet{Johnson91a,
Author = {Johnson, GA and Maki, JH},
Title = {In vivo measurement of proton diffusion in the presence of
coherent motion.},
Journal = {Invest Radiol},
Volume = {26},
Number = {6},
Pages = {540-545},
Year = {1991},
Month = {June},
ISSN = {0020-9996},
url = {http://www.ncbi.nlm.nih.gov/pubmed/1650333},
Abstract = {Measurement of the self-diffusion coefficient D of water in
tissue has been performed traditionally using the technique
proposed by Stejskal and Tanner. A variant of that technique
is shown here, employing flow-compensated gradients that
significantly reduce the sensitivity to small coherent
motions that are common in body imaging. An interleaved
sequence with four values of diffusion-sensitizing gradient
(b) minimizes registration errors. Eddy currents and other
systematic errors are reduced, permitting the measurement of
standards in an imaging context within 5% of nonimaging
values in the literature. The flow-compensated sequence
permits the measure of D for tissues in the abdominal cavity
of the rat. We present in vivo measurements of D for the
following rat tissues; liver, kidney (cortex), kidney
(medulla) muscle, brain, fat.},
Doi = {10.1097/00004424-199106000-00006},
Key = {Johnson91a}
}
@article{fds268853,
Author = {Badea, CT and Drangova, M and Holdsworth, DW and Johnson,
GA},
Title = {In vivo small-animal imaging using micro-CT and digital
subtraction angiography.},
Journal = {Phys Med Biol},
Volume = {53},
Number = {19},
Pages = {R319-R350},
Year = {2008},
Month = {October},
ISSN = {0031-9155},
url = {http://www.ncbi.nlm.nih.gov/pubmed/18758005},
Keywords = {Angiography, Digital Subtraction • Animals •
Humans • X-Ray Microtomography • instrumentation
• methods*},
Abstract = {Small-animal imaging has a critical role in phenotyping,
drug discovery and in providing a basic understanding of
mechanisms of disease. Translating imaging methods from
humans to small animals is not an easy task. The purpose of
this work is to review in vivo x-ray based small-animal
imaging, with a focus on in vivo micro-computed tomography
(micro-CT) and digital subtraction angiography (DSA). We
present the principles, technologies, image quality
parameters and types of applications. We show that both
methods can be used not only to provide morphological, but
also functional information, such as cardiac function
estimation or perfusion. Compared to other modalities, x-ray
based imaging is usually regarded as being able to provide
higher throughput at lower cost and adequate resolution. The
limitations are usually associated with the relatively poor
contrast mechanisms and potential radiation damage due to
ionizing radiation, although the use of contrast agents and
careful design of studies can address these limitations. We
hope that the information will effectively address how x-ray
based imaging can be exploited for successful in vivo
preclinical imaging.},
Doi = {10.1088/0031-9155/53/19/R01},
Key = {fds268853}
}
@article{fds174241,
Author = {CW Flanagan and RS Mannel and JL Walker and GA Johnson},
Title = {Incidence and location of para-aortic lymph node metastases
in gynecologic malignancies.},
Journal = {Journal of the American College of Surgeons},
Volume = {181},
Number = {1},
Pages = {72-4},
Year = {1995},
Month = {July},
ISSN = {1072-7515},
Keywords = {Abdomen • Endometrial Neoplasms • Female •
Genital Neoplasms, Female • Humans • Lymph Node
Excision • Lymphatic Metastasis* • Neoplasm
Staging • Ovarian Neoplasms • Retrospective
Studies • Uterine Cervical Neoplasms • pathology
• pathology*},
Abstract = {BACKGROUND: We sought to determine the location of
metastases to para-aortic lymph nodes in patients with
gynecologic malignancies. STUDY DESIGN: A retrospective
chart review was performed for all cases of endometrial,
ovarian, and cervical carcinoma in which right and left
para-aortic lymph node dissection was done at our
institution from 1985 to 1993. Records were assessed for
tumor type as well as for presence and location of
metastases to para-aortic lymph nodes. RESULTS: A total of
315 patients had bilateral para-aortic lymphadenectomy
performed at the time of laparotomy as part of staging or
therapy for their gynecologic malignancies. A total of 47
patients (15 percent) had metastasis to the para-aortic
lymph nodes. Para-aortic metastasis were identified in 22
(30 percent) of 73 patients with ovarian carcinoma, 11 (8
percent) of 141 patients with cervical carcinoma, and 14 (14
percent) of 101 patients sampled. Unilateral left-sided
para-aortic node involvement was observed in 13 patients,
unilateral right-sided involvement was present in 14
patients, and bilateral involvement occurred in 20 patients.
Regarding tumor type or origin, no significant difference
was noted in right-sided compared with left-sided
para-aortic metastases. CONCLUSIONS: Our data suggest no
difference in the incidence of metastases to right-sided
compared with left para-aortic lymph nodes in patients with
gynecologic malignancies, emphasizing the need for bilateral
evaluation of the para-aortic lymph nodes. This information
is important in the clinical staging of gynecologic
malignancies and in establishing protocols requiring
para-aortic lymph node dissection.},
Language = {eng},
Key = {fds174241}
}
@booklet{Flanagan95,
Author = {C. W. Flanagan and R. S. Mannel and J. L. Walker and G. A.
Johnson},
Title = {Incidence and location of paraaortic lymph-node metastases
in gynecologic malignancies},
Journal = {Journal Of The American College Of Surgeons},
Volume = {181},
Number = {1},
Pages = {72 -- 74},
Year = {1995},
Month = {July},
Key = {Flanagan95}
}
@article{fds174179,
Author = {GA Johnson and EG Kim},
Title = {Increase of brain levels of tryptophan induced by inhibition
of dopamine beta-hydroxylase (EC 1.14.2.1).},
Journal = {Journal of neurochemistry},
Volume = {20},
Number = {6},
Pages = {1761-4},
Year = {1973},
Month = {June},
ISSN = {0022-3042},
Keywords = {Animals • Brain • Cresols • Disulfides •
Dopamine beta-Hydroxylase • Hydroxyindoleacetic Acid
• Imidazoles • Kinetics • Male •
Norepinephrine • Oxazoles • Phenylthiourea •
Rats • Serotonin • Thiazoles • Tryptophan
• antagonists & inhibitors* • drug effects •
metabolism • metabolism* • pharmacology},
Language = {eng},
Key = {fds174179}
}
@booklet{Johnson91b,
Author = {G. A. Johnson and V. J. Kapoor and M. Shokrani and L. J.
Messick and R. Nguyen and R. A. Stall and M. A.
Mckee},
Title = {Indium gallium-arsenide microwave-power transistors},
Journal = {Ieee Transactions On Microwave Theory And
Techniques},
Volume = {39},
Number = {7},
Pages = {1069 -- 1076},
Year = {1991},
Month = {July},
Key = {Johnson91b}
}
@article{fds132904,
Author = {MJ Upfal and GA Johnson and AP Jacobson and PA Brady and JA
Campbell},
Title = {Indoor radon and lung cancer in China.},
Journal = {Journal of the National Cancer Institute, UNITED
STATES},
Volume = {82},
Number = {21},
Pages = {1722-3},
Year = {1990},
Month = {November},
ISSN = {0027-8874},
Keywords = {Air Pollutants • Carcinogens • China • Dust
• Female • Humans • Lung Neoplasms •
Radon • adverse effects • epidemiology •
etiology* • toxicity • toxicity*},
Key = {fds132904}
}
@booklet{Upfal90,
Author = {M. J. Upfal and G. A. Johnson and A. P. Jacobson and P. A.
Brady and J. A. Campbell},
Title = {Indoor radon and lung-cancer in china},
Journal = {Journal Of The National Cancer Institute},
Volume = {82},
Number = {21},
Pages = {1722 -- 1722},
Year = {1990},
Month = {November},
Key = {Upfal90}
}
@article{fds174153,
Author = {RC Ackerman and GA Johnson and EA Van Kirk and AL Asirvatham and WJ
Murdoch},
Title = {Induction of apoptotic or lytic death in an ovarian
adenocarcinoma cell line by antibodies generated against a
synthetic N-terminal extracellular domain
gonadotropin-releasing hormone receptor peptide.},
Journal = {Cancer letters},
Volume = {81},
Number = {2},
Pages = {177-84},
Year = {1994},
Month = {June},
ISSN = {0304-3835},
Keywords = {Adenocarcinoma • Amino Acid Sequence • Animals
• Apoptosis* • Cytotoxicity, Immunologic •
Female • Humans • Immune Sera • Mice •
Molecular Sequence Data • Ovarian Neoplasms •
Peptide Fragments • Receptors, LHRH • Sheep •
Tumor Cells, Cultured • immunology* •
pathology*},
Abstract = {A polyclonal antiserum was generated in ovariectomized sheep
against a synthetic peptide corresponding to amino acids
5-17 of the deduced mouse pituitary gonadotropin-releasing
hormone (GnRH) receptor. Antipeptide antibodies did not bind
native cells, but did react strongly with a human ovarian
cancer cell line (OVCAR-3) reportedly sensitive to GnRH.
Growth of cultured OVCAR-3 cells was specifically suppressed
by antipeptide serum. This was attributed in part to
programmed death (chromatin condensation and DNA
fragmentation) of cells by antibody-induced apoptosis.
Antibodies also exhibited a cytolytic effect (lactate
dehydrogenase release) toward OVCAR-3 cells in the presence
of the complement. Endometria of passively immunized mice
lacked development; thus, antipeptide antibodies evidently
recognize Mullerian duct derivatives. Experiments are in
progress to determine whether the putative antigen is a
variant of the pituitary GnRH receptor or a largely
dissimilar protein. Effector-functional antibodies could be
useful in the management of ovarian or uterine
neoplasia.},
Language = {eng},
Key = {fds174153}
}
@booklet{Ackerman94,
Author = {R. C. Ackerman and G. A. Johnson and E. A. Vankirk and A. L.
Asirvatham and W. J. Murdoch},
Title = {Induction of apoptotic or lytic death in an ovarian
adenocarcinoma cell-line by antibodies generated against a
synthetic n-terminal extracellular domain
gonadotropin-releasing-hormone receptor peptide},
Journal = {Cancer Letters},
Volume = {81},
Number = {2},
Pages = {177 -- 184},
Year = {1994},
Month = {June},
Key = {Ackerman94}
}
@booklet{Johnson91c,
Author = {G. A. Johnson and C. A. Baker and K. A. Knight},
Title = {Induction of minoxidil sulfotransferase (mst) activity
during differentiation of normal human epidermal-keratinocytes
(nheks)},
Journal = {Faseb Journal},
Volume = {5},
Number = {6},
Pages = {A1749 -- A1749},
Year = {1991},
Month = {March},
Key = {Johnson91c}
}
@booklet{Johnson92a,
Author = {G. A. Johnson and V. J. Kapoor and H. Jurgensen and D.
Schmitz},
Title = {Ingaas field-effect transistors with submicron gates for
k-band applications},
Journal = {Ieee Transactions On Microwave Theory And
Techniques},
Volume = {40},
Number = {3},
Pages = {429 -- 433},
Year = {1992},
Month = {March},
Key = {Johnson92a}
}
@article{fds371722,
Author = {Woods, HF and Stubbs, WA and Johnson, G and Alberti,
KG},
Title = {Inhibition by salicylate of gluconeogenesis in the isolated
perfused rat liver.},
Journal = {Clin Exp Pharmacol Physiol},
Volume = {1},
Number = {6},
Pages = {535-540},
Year = {1974},
url = {http://dx.doi.org/10.1111/j.1440-1681.1974.tb00574.x},
Doi = {10.1111/j.1440-1681.1974.tb00574.x},
Key = {fds371722}
}
@article{fds174222,
Author = {GA Johnson and EG Kim and SJ Boukma and D Lednicer and GA
Youngdale},
Title = {Inhibition of dopamine -hydroxylase by 5-phenoxymethyl-2-oxazolidinethiones.},
Journal = {Journal of medicinal chemistry},
Volume = {15},
Number = {3},
Pages = {327-9},
Year = {1972},
Month = {March},
ISSN = {0022-2623},
Keywords = {Adrenal Glands • Animals • Brain • Brain
Chemistry • Cattle • Dopamine beta-Hydroxylase
• Food Habits • Male • Mice • Mice,
Inbred Strains • Mixed Function Oxygenases • Motor
Activity • Norepinephrine • Oxazoles •
Phenols • Structure-Activity Relationship •
analysis • antagonists & inhibitors • antagonists
& inhibitors* • drug effects • enzymology •
pharmacology • pharmacology*},
Language = {eng},
Key = {fds174222}
}
@article{fds174273,
Author = {GA Johnson and SJ Boukma and EG Kim},
Title = {Inhibition of dopamine beta-hydroxylase by aromatic and
alkyl thioureas.},
Journal = {The Journal of pharmacology and experimental
therapeutics},
Volume = {168},
Number = {2},
Pages = {229-34},
Year = {1969},
Month = {August},
ISSN = {0022-3565},
Keywords = {Animals • Brain Chemistry • Disulfiram •
Dopamine • Mice • Mixed Function Oxygenases •
Norepinephrine • Rats • Thiourea • Tyramine
• administration & dosage • analysis •
antagonists & inhibitors* • metabolism •
pharmacology • pharmacology*},
Language = {eng},
Key = {fds174273}
}
@booklet{Johnston81,
Author = {D. G. Johnston and G. A. Johnson and H. Millwardsadler},
Title = {Insulin and liver-regeneration after partial-hepatectomy
(ph) in normal and diabetic rats},
Journal = {Diabetologia},
Volume = {21},
Number = {3},
Pages = {288 -- 288},
Year = {1981},
Key = {Johnston81}
}
@article{fds174127,
Author = {RM Simmons and DW Erikson and J Kim and RC Burghardt and FW Bazer and GA
Johnson, TE Spencer},
Title = {Insulin-like growth factor binding protein-1 in the ruminant
uterus: potential endometrial marker and regulator of
conceptus elongation.},
Journal = {Endocrinology},
Volume = {150},
Number = {9},
Pages = {4295-305},
Year = {2009},
Month = {September},
ISSN = {1945-7170},
url = {http://dx.doi.org/10.1210/en.2009-0060},
Keywords = {Animals • Cattle • Cell Movement • Cell
Proliferation • Endometrium • Female •
Insulin-Like Growth Factor Binding Protein 1 •
Insulin-Like Growth Factor Binding Protein 3 •
Interferon Type I • Pregnancy • Pregnancy Proteins
• Progesterone • Sheep • Uterus • drug
effects • metabolism • metabolism* •
pharmacology • physiology*},
Abstract = {Establishment of pregnancy in ruminants requires conceptus
elongation and production of interferon-tau (IFNT), the
pregnancy recognition signal that maintains ovarian
progesterone (P4) production. These studies determined
temporal and spatial alterations in IGF binding protein
(IGFBP)-1 and IGFBP3 in the ovine and bovine uterus; effects
of P4 and IFNT on their expression in the ovine uterus; and
effects of IGFBP1 on ovine trophectoderm cell proliferation,
migration, and attachment. IGFBP1 and IGFBP3 were studied
because they are the only IGFBPs specifically expressed by
the endometrial luminal epithelia in sheep. In sheep, IGFBP1
and IGFBP3 expression was coordinate with the period of
conceptus elongation, whereas only IGFBP1 expression was
coordinate with conceptus elongation in cattle. IGFBP1 mRNA
in the ovine endometria was between 5- and 29-fold more
abundant between d 12 and 16 of pregnancy compared with the
estrous cycle and greater on d 16 of pregnancy than
nonpregnancy in the bovine uterus. In sheep, P4 induced and
IFNT stimulated expression of IGFBP1 but not IGFBP3;
however, the effect of IFNT did not mimic the abundant
increase observed in pregnant ewes. Therefore, IGFBP1
expression in the endometrium is regulated by another factor
from the conceptus. IGFBP1 did not affect the proliferation
of ovine trophectoderm cells in vitro but did stimulate
their migration and mediate their attachment. These studies
reveal that IGFBP1 is a common endometrial marker of
conceptus elongation in sheep and cattle and most likely
regulates conceptus elongation by stimulating migration and
attachment of the trophectoderm.},
Language = {eng},
Doi = {10.1210/en.2009-0060},
Key = {fds174127}
}
@article{fds174184,
Author = {J Kim and G Song and H Gao and JL Farmer and MC Satterfield and RC
Burghardt, G Wu and GA Johnson and TE Spencer and FW
Bazer},
Title = {Insulin-like growth factor II activates phosphatidylinositol
3-kinase-protooncogenic protein kinase 1 and
mitogen-activated protein kinase cell Signaling pathways,
and stimulates migration of ovine trophectoderm
cells.},
Journal = {Endocrinology},
Volume = {149},
Number = {6},
Pages = {3085-94},
Year = {2008},
Month = {June},
ISSN = {0013-7227},
url = {http://dx.doi.org/10.1210/en.2007-1367},
Keywords = {Animals • Cell Differentiation • Cell Division
• Cloning, Molecular • Female • Insulin-Like
Growth Factor II • MAP Kinase Signaling System •
Male • Pregnancy • Proto-Oncogene Proteins c-akt
• RNA, Messenger • Ribosomal Protein S6 Kinases,
90-kDa • Sheep • Uterus • Vasectomy •
genetics • metabolism • p38 Mitogen-Activated
Protein Kinases • physiology •
physiology*},
Abstract = {IGF-II, a potent stimulator of cellular proliferation,
differentiation, and development, regulates uterine function
and conceptus growth in several species. In situ
hybridization analyses found that IGF-II mRNA was most
abundant in the caruncular endometrial stroma of both
cyclical and pregnant ewes. In the intercaruncular
endometrium, IGF-II mRNA transitioned from stroma to luminal
epithelium between d 14 and 20 of pregnancy. IGF-II mRNA was
present in all cells of the conceptus but was particularly
abundant in the yolk sac. Immunohistochemical analyses
revealed that phosphorylated (p)-protooncogenic protein
kinase 1, p-ribosomal protein S6 kinase, p-ERK1/2, and p-P38
MAPK proteins were present at low levels in a majority of
endometrial cells but were most abundant in the nuclei of
endometrial luminal epithelium and conceptus trophectoderm
of pregnant ewes. In mononuclear trophectoderm cells
isolated from d-15 conceptuses, IGF-II increased the
abundance of p-pyruvate dehydrogenase kinase 1,
p-protooncogenic protein kinase 1, p-glycogen synthase
kinase 3B, p-FK506 binding protein 12-rapamycin associated
protein 1, and p-ribosomal protein S6 kinase protein within
15 min, and the increase was maintained for 90 min. IGF-II
also elicited a rapid increase in p-ERK1/2 and p-P38 MAPK
proteins that was maximal at 15 or 30 min posttreatment.
Moreover, IGF-II increased migration of trophectoderm cells.
Collectively, these results support the hypothesis that
IGF-II coordinately activates multiple cell signaling
pathways critical to survival, growth, and differentiation
of the ovine conceptus during early pregnancy.},
Language = {eng},
Doi = {10.1210/en.2007-1367},
Key = {fds174184}
}
@article{fds268760,
Author = {Johnson, GA and Chotas, HG and Suddarth, SA and Ziv, SB and Todd,
BE},
Title = {Integrated network for medical imaging research},
Journal = {Proceedings of SPIE - The International Society for Optical
Engineering},
Volume = {1234 pt 1},
Pages = {159-166},
Year = {1990},
Abstract = {There are a number of generic problems that researchers in
medical imaging have in common. For example, researchers in
MR, CT, PET, SPECT, DSA, and digital radiography all need to
display and window digital images. We describe here an
integrated with tools applicable to all of the current areas
of medical imaging that enable researchers at Duke to share
resources and solve software and hardware problems in a
unified effort. We will show examples where efforts in a
specific area of imaging research can be readily applied in
new ways to different imaging modalities through the
facilities provided in this integrated approach. We will
point out some of the problems and opportunities in the
research environment that are different from those
encountered in clinical PACS system.},
Key = {fds268760}
}
@booklet{Johnson98d,
Author = {G. A. Johnson and T. R. Hoverstad and R. E.
Greenwald},
Title = {Integrated weed management using narrow corn row spacing,
herbicides, and cultivation},
Journal = {Agronomy Journal},
Volume = {90},
Number = {1},
Pages = {40 -- 46},
Year = {1998},
Key = {Johnson98d}
}
@article{fds174274,
Author = {PS Bridger and S Haupt and R Leiser and GA Johnson and RC Burghardt and HR
Tinneberg, C Pfarrer},
Title = {Integrin activation in bovine placentomes and in caruncular
epithelial cells isolated from pregnant cows.},
Journal = {Biology of reproduction},
Volume = {79},
Number = {2},
Pages = {274-82},
Year = {2008},
Month = {August},
ISSN = {0006-3363},
url = {http://dx.doi.org/10.1095/biolreprod.108.067637},
Keywords = {Animals • Cattle • Cell Proliferation • Cell
Separation • Cells, Cultured • Epithelial Cells
• Extracellular Matrix • Female •
Fibronectins • Focal Adhesions • Integrins •
Models, Biological • Placenta • Pregnancy •
Pregnancy, Animal* • Trophoblasts • cytology
• metabolism • metabolism* •
physiology},
Abstract = {In the bovine synepitheliochorial placenta, restricted
trophoblast invasion requires complex interactions of
integrin receptors with proteins of the extracellular matrix
(ECM) and integrin receptors of neighboring cells. Activated
integrins assemble to focal adhesions and are linked to the
actin cytoskeleton via signaling molecules including
alpha-actinin (ACTN), focal adhesion kinase (PTK2 or FAK),
phosphotyrosine, and talin (TLN1). Aims of this study were
to assess integrin activation and focal adhesion assembly
within epithelial cells of bovine placentomes and
low-passage (not transformed) placentomal caruncular
epithelial cells cultured on dishes coated with ECM
proteins. Immunofluorescence analysis was performed to
colocalize the signaling molecules ACTN, PTK2,
phosphotyrosine, and TLN1 with each other and with
beta(1)-integrin (ITGB1) in placentomal cryosections
throughout pregnancy and in caruncular epithelial cells in
vitro. Antibody specificity was confirmed by Western blot.
Cells were cultured on uncoated dishes, and the dishes were
coated with fibronectin (FN), laminin (LAMA), and collagen
type IV (COL4), thereby statistically assessing cell number
and qualitatively assessing the expression pattern of ITGB1,
phosphotyrosine, and TLN1. Results demonstrated integrin
activation and focal adhesion assembly in the placentome and
that low-passage caruncular epithelial cells maintain
integrin-associated properties observed in vivo. Expression
and/or colocalization of signaling molecules with ITGB1
confirmed, for the first time, integrin activation and
participation in "outside-in" and "inside-out" signaling
pathways. The prominent role of ECM, and FN in particular,
in integrin signaling is supported by the in vitro
enhancement of proliferation and focal adhesion expression.
Thus, this in vitro model provides excellent potential for
further mechanistic studies designed to elucidate
feto-maternal interactions in the bovine
placentome.},
Language = {eng},
Doi = {10.1095/biolreprod.108.067637},
Key = {fds174274}
}
@booklet{Burghardt02,
Author = {R. C. Burghardt and G. A. Johnson and L. A. Jaeger and H. Ka and J. E. Garlow and T. E. Spencer and F. W.
Bazer},
Title = {Integrins and extracellular matrix proteins at the
maternal-fetal interface in domestic animals},
Journal = {Cells Tissues Organs},
Volume = {172},
Number = {3},
Pages = {202 -- 217},
Year = {2002},
Key = {Burghardt02}
}
@article{fds174284,
Author = {RC Burghardt and GA Johnson and LA Jaeger and H Ka and JE Garlow and TE
Spencer, FW Bazer},
Title = {Integrins and extracellular matrix proteins at the
maternal-fetal interface in domestic animals.},
Journal = {Cells, tissues, organs},
Volume = {172},
Number = {3},
Pages = {202-17},
Year = {2002},
ISSN = {1422-6405},
Keywords = {Animals • Embryo Implantation • Endometrium •
Extracellular Matrix Proteins • Female • Humans
• Integrins • Maternal-Fetal Exchange* •
Osteopontin • Peptide Fragments • Placenta •
Pregnancy • Protein Precursors • Sheep •
Sialoglycoproteins • Swine • Transforming Growth
Factor beta1 • cytology • genetics •
metabolism • metabolism* • physiology •
physiology*},
Abstract = {Establishment of pregnancy in mammals requires coordinated
conceptus-maternal interactions involving numerous hormones,
growth factors and cytokines acting via specific receptors
in the uterus. Uterine secretions play an important role in
establishing synchrony between development of the conceptus
and uterine receptivity, as well as in conceptus remodeling,
adhesion, implantation and placentation in domestic species.
Studies of non-invasive implantation in domestic livestock
provide valuable opportunities to investigate fundamental
processes of the initial events of apposition, attachment
and adhesive interactions that are shared among species. In
pigs and sheep, it appears that integrins play a dominant
role in these fundamental processes via interactions with
extracellular matrix molecules and other ligands to
transduce cellular signals in uterine epithelial cells and
conceptus trophectoderm. This review considers several of
the potential integrin-binding ligands involved in the
complex implantation adhesion cascade in pigs and sheep
along with in vitro evidence for the transduction of
cytoplasmic signals that may be required to sustain fetal
and maternal contributions to the formation of the
epitheliochorial placenta.},
Language = {eng},
Key = {fds174284}
}
@booklet{Buhl92,
Author = {A. E. Buhl and T. T. Kawabe and D. K. Maccallum and D. J.
Waldon and K. A. Knight and G. A. Johnson},
Title = {Interaction of minoxidil with pigment in cells of the hair
follicle - an example of binding without apparent biological
effects},
Journal = {Skin Pharmacology},
Volume = {5},
Number = {2},
Pages = {114 -- 123},
Year = {1992},
Key = {Buhl92}
}
@article{fds174260,
Author = {AE Buhl and TT Kawabe and DK MacCallum and DJ Waldon and KA Knight and GA
Johnson},
Title = {Interaction of minoxidil with pigment in cells of the hair
follicle: an example of binding without apparent biological
effects.},
Journal = {Skin pharmacology : the official journal of the Skin
Pharmacology Society},
Volume = {5},
Number = {2},
Pages = {114-23},
Year = {1992},
ISSN = {1011-0283},
Keywords = {Animals • Autoradiography • Cell Differentiation
• Cell Division • Cysteine • Melanins •
Melanocyte-Stimulating Hormones • Mice • Mice,
Inbred C3H • Mice, Inbred C57BL • Mice, Inbred
Strains • Minoxidil • Pigments, Biological •
Protein Binding • Thymidine • Vibrissae •
drug effects • metabolism • metabolism* •
pharmacokinetics* • pharmacology},
Abstract = {To identify minoxidil target cells in hair follicles we
followed the uptake of radiolabeled drug in mouse vibrissae
follicles both in vitro and in vivo. Autoradiography showed
that both 3H-minoxidil and 3H-minoxidil sulfate accumulated
in the differentiating epithelial matrix cells superior to
the dermal papilla, a distribution similar to that of
pigment. Minoxidil localized in melanocytes, melanocyte
processes, and areas of greater melanin concentrations
within the epithelial cells. Although uptake of minoxidil
was significantly less in unpigmented follicles, the drug
stimulated proliferation and differentiation of both
pigmented and unpigmented follicles. Labeled minoxidil bound
to Sepia melanin and was displaced with unlabeled minoxidil
and other electron donor drugs. This interaction with
melanin acts as a targeting mechanism of minoxidil to
pigmented hair follicles but has no apparent functional
significance in hair growth. This work illustrates how
measurement of drugs in hair may be biased by
pigmentation.},
Language = {eng},
Key = {fds174260}
}
@booklet{Choi01,
Author = {Y. S. Choi and G. A. Johnson and R. C. Burghardt and L. R.
Berghman and M. M. Joyce and K. M. Taylor and M. D. Stewart and F. W. Bazer and T. E. Spencer},
Title = {Interferon regulatory factor-two restricts expression of
interferon-stimulated genes to the endometrial stroma and
glandular epithelium of the ovine uterus},
Journal = {Biology Of Reproduction},
Volume = {65},
Number = {4},
Pages = {1038 -- 1049},
Year = {2001},
Month = {October},
Key = {Choi01}
}
@article{fds174124,
Author = {Y Choi and GA Johnson and RC Burghardt and LR Berghman and MM Joyce and KM
Taylor, MD Stewart and FW Bazer and TE Spencer},
Title = {Interferon regulatory factor-two restricts expression of
interferon-stimulated genes to the endometrial stroma and
glandular epithelium of the ovine uterus.},
Journal = {Biology of reproduction},
Volume = {65},
Number = {4},
Pages = {1038-49},
Year = {2001},
Month = {October},
ISSN = {0006-3363},
Keywords = {Animals • DNA-Binding Proteins • Endometrium
• Epithelium • Estrous Cycle • Female •
Fluorescent Antibody Technique • Gene Expression*
• Interferon Regulatory Factor-1 • Interferon
Regulatory Factor-2 • Interferon Type I •
Interferon-Stimulated Gene Factor 3 • Interferons
• Phosphoproteins • Pregnancy Proteins •
Promoter Regions, Genetic • RNA, Messenger •
Recombinant Proteins • Repressor Proteins* • STAT1
Transcription Factor • STAT2 Transcription Factor
• Sheep* • Stromal Cells • Trans-Activators
• Transcription Factors • Transcriptional
Activation • Transfection • Uterus • analysis
• drug effects • genetics • metabolism •
metabolism* • pharmacology • pharmacology* •
physiology • physiology*},
Abstract = {Interferon tau (IFNtau) is the signal for maternal
recognition of pregnancy in ruminants. The positive effects
of IFNtau on IFN-stimulated gene (ISG) expression are
mediated by ISG factor 3 (ISGF3), which is composed of
signal transducer and activator of transcription (Stat) 1,
Stat 2, and IFN regulatory factor-9 (IRF-9), and by
gamma-activated factor (GAF), which is a Stat 1 homodimer.
Induction of ISGs, such as ISG17 and 2',5'-oligoadenylate
synthetase, by IFNtau during pregnancy is limited to the
endometrial stroma (S) and glandular epithelium (GE) of the
ovine uterus. The IRF-2, a potent transcriptional repressor
of ISG expression, is expressed in the luminal epithelium
(LE). This study determined effects of the estrous cycle,
pregnancy, and IFNtau on expression of Stat 1, Stat 2,
IRF-9, IRF-1, and IRF-2 genes in the ovine endometrium. In
cyclic ewes, Stat 1, Stat 2, IRF-1, and IRF-9 mRNA and
protein were detected at low levels in the S and GE. During
pregnancy, expression of these genes increased only in the S
and GE. Expression of IRF-2 was detected only in the LE and
superficial GE (sGE) of both cyclic and pregnant ewes. In
cyclic ewes, intrauterine administration of IFNtau
stimulated Stat 1, Stat 2, IRF-9, and IRF-1 expression in
the endometrium. Ovine IRF-2 repressed transcriptional
activity driven by IFN-stimulated response elements that
bind ISGF3, but not by gamma-activation sequences that bind
GAF. These results suggest that IRF-2 in the LE and sGE
restricts IFNtau induction of ISGs to the S and GE. In the S
and GE, IFNtau hyperactivation of ISG expression likely
involves formation and actions of the transcription factors
ISGF3 and, perhaps, IRF-1.},
Language = {eng},
Key = {fds174124}
}
@article{fds174113,
Author = {MM Joyce and FJ White and RC Burghardt and JJ Muñiz and TE Spencer and FW
Bazer, GA Johnson},
Title = {Interferon stimulated gene 15 conjugates to endometrial
cytosolic proteins and is expressed at the uterine-placental
interface throughout pregnancy in sheep.},
Journal = {Endocrinology},
Volume = {146},
Number = {2},
Pages = {675-84},
Year = {2005},
Month = {February},
ISSN = {0013-7227},
url = {http://dx.doi.org/10.1210/en.2004-1224},
Keywords = {Animals • Blotting, Western • Cytokines •
Cytosol • Endometrium • Female • Gene
Expression • Immunohistochemistry • In Situ
Hybridization • Interferon Type I • Male •
Placenta • Pregnancy • Pregnancy Proteins •
RNA, Messenger • Sheep • Ubiquitin • analogs
& derivatives* • analysis • genetics •
genetics* • metabolism • metabolism* •
physiology*},
Abstract = {Interferon-stimulated gene 15 (ISG15) is a ubiquitin homolog
expressed in uteri of ruminants in response to interferon
(IFN)-tau and is also induced during pregnancy in the uteri
of mice, pigs, humans, and baboons. This study examined
expression of ISG15 and its conjugation to target proteins
in the ovine uterus beyond the period of IFNtau secretion by
the conceptus. Although steady-state levels of ISG15 mRNA
decreased after d 25 of pregnancy, ISG15 persisted in
endometrium through d 120. In situ hybridization and
immunocytochemistry localized ISG15 across the entire
uterine wall through d 25, after which expression was
restricted to endometrial stroma along the
maternal-placental interface. Western blots revealed ISG15
and ISG15-conjugated proteins in endometrium. Treatment of
ovariectomized sheep with progesterone and IFNtau increased
both free and conjugated ISG15. These results are the first
to show in vivo regulation of ISG15 function (i.e.
conjugation to target proteins) by a type I IFN in the
uterus of any species and that ISG15 is expressed at
contacts between the placenta and uterus when trophectoderm
no longer produces IFNtau. Interestingly, mRNA for the type
II IFNgamma was present in the endometrial stromal
compartment on d 15-50, which may stimulate the synthesis of
ISG15 through later pregnancy. We hypothesize that ISG15 is
not merely a consequence of an antiviral state induced by
trophoblast IFNtau but represents a critical component of
the microenvironment at the uterine-placental interface
during the progressive events of conceptus development,
implantation, and placentation in sheep and perhaps other
mammalian species.},
Language = {eng},
Doi = {10.1210/en.2004-1224},
Key = {fds174113}
}
@booklet{Bazer00,
Author = {F. W. Bazer and J. A. G. W. Fleming and G. A. Johnson and Y.
S. Choi and M. D. Stewart and T. E. Spencer},
Title = {Interferon tau inhibits transcription of the ovine estrogen
receptor alpha gene: Involvement of STATs and
IRFs.},
Journal = {Biology Of Reproduction},
Volume = {62},
Pages = {292 -- 292},
Year = {2000},
Key = {Bazer00}
}
@booklet{Joyce02,
Author = {M. M. Joyce and T. R. Hansen and G. A. Johnson},
Title = {Interferon-stimulated gene 17 is expressed in the porcine
uterus and may be critical to placental development across
species.},
Journal = {Biology Of Reproduction},
Volume = {66},
Pages = {185 -- 186},
Year = {2002},
Key = {Joyce02}
}
@booklet{Joyce03,
Author = {M. M. Joyce and R. C. Burghardt and F. W. Bazer and G. M.
Zaunbrecher and G. A. Johnson},
Title = {Interferon-stimulated genes (ISGs) are induced in the
endometrium of pregnant but not pseudopregnant
pigs.},
Journal = {Biology Of Reproduction},
Volume = {68},
Pages = {206 -- 206},
Year = {2003},
Key = {Joyce03}
}
@booklet{Stewart01,
Author = {M. D. Stewart and G. A. Johnson and F. W. Bazer and T. E.
Spencer},
Title = {Interferon-tau (IFN tau) regulation of IFN-stimulated gene
expression in cell lines lacking specific IFN-signaling
components},
Journal = {Endocrinology},
Volume = {142},
Number = {5},
Pages = {1786 -- 1794},
Year = {2001},
Month = {May},
Key = {Stewart01}
}
@article{fds174144,
Author = {MD Stewart and GA Johnson and FW Bazer and TE Spencer},
Title = {Interferon-tau (IFNtau) regulation of IFN-stimulated gene
expression in cell lines lacking specific IFN-signaling
components.},
Journal = {Endocrinology},
Volume = {142},
Number = {5},
Pages = {1786-94},
Year = {2001},
Month = {May},
ISSN = {0013-7227},
Keywords = {2',5'-Oligoadenylate Synthetase • Cell Line •
DNA-Binding Proteins • Fibroblasts • Gene
Expression Regulation • Humans • Interferon Type I
• Interferon-Stimulated Gene Factor 3 •
Interferon-Stimulated Gene Factor 3, gamma Subunit •
Phosphorylation • Pregnancy Proteins • STAT1
Transcription Factor • STAT2 Transcription Factor
• STAT3 Transcription Factor • Trans-Activators
• Transcription Factors • biosynthesis • drug
effects* • genetics* • metabolism •
pharmacology* • physiology},
Abstract = {Interferon-tau (IFNtau) is a unique type I IFN secreted by
the ruminant conceptus that acts in a paracrine manner on
the endometrial epithelium to signal pregnancy recognition.
In the ovine endometrium, IFNtau suppresses estrogen
receptor alpha and oxytocin receptor gene expression, but
increases or induces expression of IFN-simulated genes
(ISGs), including signal transducer and activator of
transcription-1 (STAT1), STAT2, ISG factor-3gamma
(ISGF3gamma)/p48/IFN regulatory factor-9, and
2',5'-oligoadenylate synthetase (OAS). Human fibroblast cell
lines lacking specific IFN signaling components were
employed to determine the roles of STAT1, STAT2, and
ISGF3gamma in the effects of IFNtau on ISG protein
expression. Results indicated that STAT1alpha or STAT1beta
is required for IFNtau effects on STAT2, ISGF3gamma, and OAS
(40/46, 69/71, and 100 kDa). STAT2 is required for effects
on STAT1, ISGF3gamma, and all OAS forms. ISGF3gamma is
required for effects of IFNtau on STAT2 and 40/46- and
69/71-kDa OAS and plays a role in the effects of IFNtau on
100-kDa OAS and STAT1. Mutation of Tyr(701), but not
Ser(727), of STAT1 abolished the effects of IFNtau on ISG
expression. Mutation of the SH2 domain of STAT1 abolished
the effects of IFNtau on all ISGs and reduced increases in
100-kDa OAS. These data illustrate the importance of
transcription factors composed of STAT1, STAT2, and
ISGF3gamma in the signaling pathway mediating the effects of
IFNtau on ISG expression.},
Language = {eng},
Key = {fds174144}
}
@booklet{Stewart01a,
Author = {D. M. Stewart and G. A. Johnson and C. A. Vyhlidal and R. C.
Burghardt and S. H. Safe and L. Y. Yu-lee and F. W. Bazer and T. E. Spencer},
Title = {Interferon-tau activates multiple signal transducer and
activator of transcription proteins and has complex effects
on interferon-responsive gene transcription in ovine
endometrial epithelial cells},
Journal = {Endocrinology},
Volume = {142},
Number = {1},
Pages = {98 -- 107},
Year = {2001},
Month = {January},
Key = {Stewart01a}
}
@article{fds174117,
Author = {DM Stewart and GA Johnson and CA Vyhlidal and RC Burghardt and SH Safe and LY Yu-Lee and FW Bazer and TE Spencer},
Title = {Interferon-tau activates multiple signal transducer and
activator of transcription proteins and has complex effects
on interferon-responsive gene transcription in ovine
endometrial epithelial cells.},
Journal = {Endocrinology},
Volume = {142},
Number = {1},
Pages = {98-107},
Year = {2001},
Month = {January},
ISSN = {0013-7227},
Keywords = {Animals • Cell Nucleus • Cells, Cultured •
DNA-Binding Proteins • Endometrium • Epithelial
Cells • Female • Interferon Type I •
Interferon-Stimulated Gene Factor 3 • Luciferases
• Phosphorylation • Pregnancy Proteins •
Promoter Regions, Genetic • Protein Transport •
Recombinant Fusion Proteins • STAT1 Transcription
Factor • STAT2 Transcription Factor • Sheep •
Signal Transduction • Trans-Activators •
Transcription Factors • Transcription, Genetic •
Transfection • analysis • cytology • drug
effects • genetics* • metabolism •
metabolism* • pharmacology* • physiology •
physiology*},
Abstract = {Interferon-tau (IFNtau), a type I IFN produced by sheep
conceptus trophectoderm, is the signal for maternal
recognition of pregnancy. Although it is clear that IFNtau
suppresses transcription of the estrogen receptor alpha and
oxytocin receptor genes and induces expression of various
IFN-stimulated genes within the endometrial epithelium,
little is known of the signal transduction pathway activated
by the hormone. This study determined the effects of IFNtau
on signal transducer and activator of transcription (STAT)
activation, expression, DNA binding, and transcriptional
activation using an ovine endometrial epithelial cell line.
IFNtau induced persistent tyrosine phosphorylation and
nuclear translocation of STAT1 and -2 (10 min to 48 h), but
transient phosphorylation and nuclear translocation of
STAT3, -5a/b, and -6 (10 to <60 min). IFNtau increased
expression of STAT1 and -2, but not STAT3, -5a/b, and -6.
IFN-stimulated gene factor-3 and STAT1 homodimers formed and
bound an IFN-stimulated response element (ISRE) and
gamma-activated sequence (GAS) element, respectively. IFNtau
increased transcription of GAS-driven promoters at 3 h, but
suppressed their activity at 24 h. In contrast, the activity
of an ISRE-driven promoter was increased at 3 and 24 h.
These results indicate that IFNtau activates multiple STATs
and has differential effects on ISRE- and GAS-driven gene
transcription.},
Language = {eng},
Key = {fds174117}
}
@booklet{Johnson00d,
Author = {G. A. Johnson and T. E. Spencer and R. C. Burghardt and M.
M. Joyce and F. W. Bazer},
Title = {Interferon-tau and progesterone regulate ubiquitin
cross-reactive protein expression in the ovine
uterus},
Journal = {Biology Of Reproduction},
Volume = {62},
Number = {3},
Pages = {622 -- 627},
Year = {2000},
Month = {March},
Key = {Johnson00d}
}
@article{fds174133,
Author = {GA Johnson and TE Spencer and RC Burghardt and MM Joyce and FW
Bazer},
Title = {Interferon-tau and progesterone regulate ubiquitin
cross-reactive protein expression in the ovine
uterus.},
Journal = {Biology of reproduction},
Volume = {62},
Number = {3},
Pages = {622-7},
Year = {2000},
Month = {March},
ISSN = {0006-3363},
Keywords = {Animals • Blotting, Western • Female •
Hormone Antagonists • Hysterectomy • In Situ
Hybridization • Interferon Type I • Pregnancy
Proteins • Progesterone • Sheep • Ubiquitins
• Uterus • analogs & derivatives* •
antagonists & inhibitors • drug effects • genetics
• metabolism • metabolism* • pharmacology
• physiology},
Abstract = {Ubiquitin cross-reactive protein (UCRP) is a functional
ubiquitin homolog synthesized by the ruminant endometrium in
response to conceptus-derived interferon-tau (IFNtau).
Progesterone is required for IFNtau to exert antiluteolytic
actions on the endometrium. Therefore, this study was
designed to determine whether progesterone is requisite for
IFNtau induction of UCRP expression within the ovine uterus.
Cyclic ewes were ovariectomized and fitted with intrauterine
(i.u.) catheters on Day 5 and treated daily with steroids
(i.m.) and protein (i.u.) as follows: 1) progesterone (P,
Days 5-24) and control serum proteins (CX, Days 11-24); 2) P
and ZK 137.316 (ZK; progesterone receptor antagonist, Days
11-24) and CX proteins; 3) P and recombinant ovine IFNtau
(roIFNtau, Days 11-24); or 4) P and ZK and roIFNtau. All
ewes were hysterectomized on Day 25. In P-treated ewes,
roIFNtau increased endometrial UCRP mRNA and protein levels.
However, administration of ZK to ewes ablated roIFNtau
induction of UCRP. Recombinant ovine IFNtau induced
expression of UCRP mRNA in progestinized endometrial luminal
(LE) and glandular (GE) epithelium as well as in both
stratum compactum and spongiosum layers of the stroma (ST).
Progesterone receptor protein was located in endometrial ST,
but not in LE and GE from these ewes. Results support the
hypothesis that progesterone is required for IFNtau
induction of type I IFN-responsive genes, such as UCRP, in
the ruminant uterus.},
Language = {eng},
Key = {fds174133}
}
@booklet{Staggs97,
Author = {K. L. Staggs and K. J. Austin and G. A. Johnson and C. T.
Talbott and T. R. Hansen},
Title = {Interferon-tau may elicit specific responses in the
endometrium through signal transduction pathways other than
the Jak/Stat system.},
Journal = {Biology Of Reproduction},
Volume = {56},
Pages = {159 -- 159},
Year = {1997},
Key = {Staggs97}
}
@article{fds174185,
Author = {FW Bazer and RC Burghardt and GA Johnson and TE Spencer and G
Wu},
Title = {Interferons and progesterone for establishment and
maintenance of pregnancy: interactions among novel cell
signaling pathways.},
Journal = {Reproductive biology},
Volume = {8},
Number = {3},
Pages = {179-211},
Year = {2008},
Month = {November},
ISSN = {1642-431X},
Keywords = {Animals • Embryo Implantation • Estrogens •
Female • Humans • Interferon Type I •
Interferons • Pregnancy • Pregnancy Maintenance
• Pregnancy Proteins • Progesterone • Signal
Transduction • Swine • Uterus • drug effects
• physiology • physiology*},
Abstract = {Type I and/or type II interferons (IFNs) are important in
establishing uterine receptivity to implantation in mammals.
Gene expression effected by IFNs may be induced, stimulated
or inhibited, but most are IFN-stimulated genes (ISGs).
Effects of IFNs range from pregnancy recognition signaling
in ruminants by IFN tau (IFNT) to effects on cellular
functions of the uterus and uterine vasculature. For most,
if not all, actions of IFNs on the uterus, progesterone
(P(4)) is permissive to ISG expression, with genes being
induced by IFN or induced by P(4) and stimulated by IFN.
Uterine receptivity to implantation is P(4)-dependent;
however, implantation events are preceded by loss of
expression of progesterone (PGR) and estrogen (ESR1)
receptors by uterine epithelia. Thus, P4 likely stimulates
PGR-positive stromal cells to express one or more
progestamedins, e.g., fibroblast growth factors-7 and -10,
and/or hepatocyte growth factor, that act via their
respective receptors on uterine epithelia and trophectoderm
to regulate expression of ISGs. FGF10 appears to be the most
important progestamedin in sheep uteri during pregnancy.
Sequential effects of P(4) to induce and IFNs to stimulate
gene expression suggest that P(4) and IFNs activate
complimentary cell signaling pathways to modulate expression
of genes for attachment of trophectoderm to uterine lumenal
and superficial glandular epithelia (LE/sGE), modify
phenotype of uterine stromal cells, silence PGR and ESR1
genes, signal pregnancy recognition, suppress genes for
immune recognition, alter membrane permeability to enhance
conceptus-maternal exchange of factors, increase endometrial
vascularity and activate genes for transport of nutrients
into the uterine lumen. In ewes, IFNT abrogrates the uterine
luteolytic mechanism and stimulates expression of classical
ISGs by GE and stromal cells, whereas LE/sGE express
P(4)-induced and IFNT-stimulated genes important for uterine
receptivity to implantation and conceptus development. These
include wingless-type MMTV (mouse mammary tumor virus)
integration site family member 7A (WNT7A) induced by IFNT,
as well as galectin, proteases, protease inhibitors,
transporters for glucose and amino acids, gastrin releasing
polypeptide, insulin-like growth factor binding protein 1
and a hypoxia inducible factor. The specific functions of
IFNs and ISGs induced in primates, pigs and other mammals
during pregnancy are not known, but likely are important in
establishment of pregnancy. Understanding the roles of IFNs
and ISGs in uterine receptivity for implantation is
necessary to develop strategies to enhance reproductive
health and fertility in humans and domestic animals. The
magnitude of the LH surge was reduced in cows receiving
endotoxin.},
Language = {eng},
Key = {fds174185}
}
@article{fds174306,
Author = {FW Bazer and TE Spencer and GA Johnson},
Title = {Interferons and uterine receptivity.},
Journal = {Seminars in reproductive medicine},
Volume = {27},
Number = {1},
Pages = {90-102},
Year = {2009},
Month = {January},
ISSN = {1526-4564},
url = {http://dx.doi.org/10.1055/s-0028-1108013},
Keywords = {Animals • Embryo Implantation • Estrogens •
Female • Interferons • Models, Biological •
Pregnancy • Primates • Rodentia • Ruminants
• Swine • Uterus • physiology •
physiology*},
Abstract = {This article focuses on the potential roles of interferons
(IFNs) in establishing uterine receptivity to implantation.
A common feature of the peri-implantation period of
pregnancy in most mammals is production of type I and/or
type II IFNs by trophoblasts that induce and/or stimulate
expression of an array of IFN-stimulate genes (ISGs). These
effects range from pregnancy recognition signaling in
ruminants through IFN tau to effects on cellular functions
of the uterus and uterine vasculature. For actions of IFNs,
progesterone (P4) is permissive to the expression of many
effects and to the expression of ISGs that are induced
directly by an IFN or induced by P4 and stimulated by an IFN
in a temporal and/or cell-specific manner. Uterine
receptivity to implantation is P4 dependent; however,
implantation events are preceded by loss of expression of
progesterone (PGR) and estrogen (ESR1) receptors by uterine
epithelia. Therefore, P4 likely acts via PGR-positive
stromal cells to induce expression of fibroblast growth
factors-7 and -10 and/or hepatocyte growth factor
(progestamedins) that then act via their respective
receptors on uterine epithelia and trophectoderm to affect
expression of ISGs. The permissive effects of P4 on the
expression of ISGs and the effects of P4 to induce and IFNs
to stimulate gene expression raise the question of whether
uterine receptivity to implantation requires P4 and IFN to
activate unique, but complementary, cell signaling pathways.
Uterine receptivity to implantation, depending on species,
involves changes in the expression of genes for the
attachment of trophectoderm to the uterine lumenal
epithelium (LE) and superficial glandular epithelium (sGE),
modification of the phenotype of uterine stromal cells, the
silencing of PGR and ESR1 genes, the suppression of genes
for immune recognition, alterations in membrane permeability
to enhance conceptus-maternal exchange of factors, increased
vascularity of the endometrium, activation of genes for
transport of nutrients into the uterine lumen, and enhanced
signaling for pregnancy recognition. Differential expression
of genes by uterine LE/sGE, mid- to deep-glandular epithelia
(GE), and stromal cells in response to P4 and IFNs is likely
to influence uterine receptivity to implantation in most
mammals. Understanding the roles of IFNs in uterine
receptivity for implantation is necessary to develop
approaches to enhance reproductive health and fertility in
humans and domestic animals.},
Language = {eng},
Doi = {10.1055/s-0028-1108013},
Key = {fds174306}
}
@article{fds268849,
Author = {Goddeeris, MM and Rho, S and Petiet, A and Davenport, CL and Johnson,
GA and Meyers, EN and Klingensmith, J},
Title = {Intracardiac septation requires hedgehog-dependent cellular
contributions from outside the heart.},
Journal = {Development},
Volume = {135},
Number = {10},
Pages = {1887-1895},
Year = {2008},
Month = {May},
ISSN = {0950-1991},
url = {http://www.ncbi.nlm.nih.gov/pubmed/18441277},
Keywords = {Animals • Fetal Heart • Heart Atria • Heart
Septal Defects, Ventricular • Hedgehog Proteins •
Mesoderm • Mice • Mice, Mutant Strains •
Mutation • Signal Transduction • cytology •
cytology* • embryology • genetics •
metabolism*},
Abstract = {Septation of the mammalian heart into four chambers requires
the orchestration of multiple tissue progenitors.
Abnormalities in this process can result in potentially
fatal atrioventricular septation defects (AVSD). The
contribution of extracardiac cells to atrial septation has
recently been recognized. Here, we use a genetic marker and
novel magnetic resonance microscopy techniques to
demonstrate the origins of the dorsal mesenchymal protrusion
in the dorsal mesocardium, and its substantial contribution
to atrioventricular septation. We explore the functional
significance of this tissue to atrioventricular septation
through study of the previously uncharacterized AVSD
phenotype of Shh(-/-) mutant mouse embryos. We demonstrate
that Shh signaling is required within the dorsal mesocardium
for its contribution to the atria. Failure of this addition
results in severe AVSD. These studies demonstrate that AVSD
can result from a primary defect in dorsal mesocardium,
providing a new paradigm for the understanding of human
AVSD.},
Doi = {10.1242/dev.016147},
Key = {fds268849}
}
@article{fds174231,
Author = {DA Massuto and RN Hooper and EC Kneese and GA Johnson and NH Ing and BR
Weeks, LA Jaeger},
Title = {Intrauterine infusion of latency-associated peptide (LAP)
during early porcine pregnancy affects conceptus elongation
and placental size.},
Journal = {Biology of reproduction},
Volume = {82},
Number = {3},
Pages = {534-42},
Year = {2010},
Month = {March},
ISSN = {1529-7268},
url = {http://dx.doi.org/10.1095/biolreprod.109.081893},
Abstract = {In the pig, transforming growth factor beta (TGFB), TGFB
receptors (TGFBRs), and integrins are present during the
peri-implantation period. Latency-associated peptide (LAP),
a part of latent TGFB, can bind to integrin heterodimers via
its Arg-Gly-Asp (RGD) sequence; therefore, ligand-receptor
interactions between TGFB and TGFBRs, along with LAP and
integrin heterodimers, may be functional in mediating events
supporting conceptus elongation and attachment. With the use
of surgically implantable osmotic pumps, we were able to
maintain pregnancy with the aim of mechanistically altering
in vivo receptor-ligand interactions involving TGFB with
TGFBRs and LAP with integrins during porcine pregnancy. Day
9 pregnant gilts received intrauterine infusions of LAP-RGD,
a recombinant mutant of LAP (LAP-RGE), or vehicle control
and were ovariohysterectomized on Day 13 or 24 of pregnancy.
We hypothesized that intrauterine infusion of LAP-RGD would
decrease downstream signaling of TGFB while increasing
LAP-integrin interactions and that net effect would enhance
conceptus survival and attachment early in the
peri-implantation period but possibly increase the chance of
abnormal placentation later in pregnancy. Additionally, we
hypothesized that infusion of LAP-RGE would disrupt TGFB
signals but not alter integrin signaling, and thus the net
result would be decreased conceptus survival and abnormal
development. Unexpectedly, LAP-RGD intrauterine infusions
resulted in a reduction of conceptus elongation, whereas
infusions of LAP-RGE permitted implantation and placentation
but resulted in larger fetal weight, allantois length, and
allantoic fluid volume. Results suggest TGFB and integrins
are contributing factors in the regulation of conceptus
elongation and placental and fetal size.},
Language = {eng},
Doi = {10.1095/biolreprod.109.081893},
Key = {fds174231}
}
@booklet{Johnson85c,
Author = {G. A. Johnson},
Title = {Inverse no-matrices},
Journal = {Linear Algebra And Its Applications},
Volume = {64},
Number = {JAN},
Pages = {215 -- 222},
Year = {1985},
Key = {Johnson85c}
}
@article{fds268712,
Author = {Calabrese, E and Badea, A and Coe, CL and Lubach, GR and Styner, MA and Johnson, GA},
Title = {Investigating the tradeoffs between spatial resolution and
diffusion sampling for brain mapping with diffusion
tractography: time well spent?},
Journal = {Hum Brain Mapp},
Volume = {35},
Number = {11},
Pages = {5667-5685},
Year = {2014},
Month = {November},
ISSN = {1065-9471},
url = {http://dx.doi.org/10.1002/hbm.22578},
Abstract = {Interest in mapping white matter pathways in the brain has
peaked with the recognition that altered brain connectivity
may contribute to a variety of neurologic and psychiatric
diseases. Diffusion tractography has emerged as a popular
method for postmortem brain mapping initiatives, including
the ex-vivo component of the human connectome project, yet
it remains unclear to what extent computer-generated tracks
fully reflect the actual underlying anatomy. Of particular
concern is the fact that diffusion tractography results vary
widely depending on the choice of acquisition protocol. The
two major acquisition variables that consume scan time,
spatial resolution, and diffusion sampling, can each have
profound effects on the resulting tractography. In this
analysis, we determined the effects of the temporal tradeoff
between spatial resolution and diffusion sampling on
tractography in the ex-vivo rhesus macaque brain, a close
primate model for the human brain. We used the wealth of
autoradiography-based connectivity data available for the
rhesus macaque brain to assess the anatomic accuracy of six
time-matched diffusion acquisition protocols with varying
balance between spatial and diffusion sampling. We show that
tractography results vary greatly, even when the subject and
the total acquisition time are held constant. Further, we
found that focusing on either spatial resolution or
diffusion sampling at the expense of the other is
counterproductive. A balanced consideration of both sampling
domains produces the most anatomically accurate and
consistent results.},
Doi = {10.1002/hbm.22578},
Key = {fds268712}
}
@article{fds359552,
Author = {Johnson, GA and O’Foghludha, F},
Title = {Investigation of transmolybdenum fluorescent anodes for
mammography},
Journal = {Proceedings of SPIE - The International Society for Optical
Engineering},
Volume = {233},
Pages = {158-163},
Year = {1980},
Month = {August},
url = {http://dx.doi.org/10.1117/12.958917},
Abstract = {Recent work has suggested a new approach to film/screen
mammography through the use of transmolybdenum (Z > 42)
fluorescent anodes. An experimental rhodium (Z = 45) anode
with K emission at 20.0 - 23.2 keV has been compared to a
conventional molybdenum anode. Previous spectral
measurements have indicated a dose advantage for the Rh
anode but no comparison of contrast has been made. A series
of step wedges of different materials was imaged at several
kVp and for several phantom thicknesses. The resulting data
permits quantitative comparison of the radiographic
contrast. At 40 kVp for an equivalent tissue thickness of
4.8 cm the radiographic contrast provided by the Rh anode is
at least equal to and under some conditions actually
superior to that provided by a Mo anode. © 1980
SPIE.},
Doi = {10.1117/12.958917},
Key = {fds359552}
}
@article{fds268787,
Author = {Badea, CT and Stanton, IN and Johnston, SM and Johnson, GA and Therien,
MJ},
Title = {Investigations on X-ray luminescence CT for small animal
imaging.},
Journal = {Proc SPIE Int Soc Opt Eng},
Volume = {8313},
Pages = {83130T},
Year = {2012},
ISSN = {0277-786X},
url = {http://www.ncbi.nlm.nih.gov/pubmed/23227300},
Abstract = {X-ray Luminescence CT (XLCT) is a hybrid imaging modality
combining x-ray and optical imaging in which x-ray
luminescent nanophosphors (NPs) are used as emissive imaging
probes. NPs are easily excited using common CT energy x-ray
beams, and the NP luminescence is efficiently collected
using sensitive light based detection systems. XLCT can be
recognized as a close analog to fluorescence diffuse optical
tomography (FDOT). However, XLCT has remarkable advantages
over FDOT due to the substantial excitation penetration
depths provided by x-rays relative to laser light sources,
long term photo-stability of NPs, and the ability to tune NP
emission within the NIR spectral window. Since XCLT uses an
x-ray pencil beam excitation, the emitted light can be
measured and back-projected along the x-ray path during
reconstruction, where the size of the X-ray pencil beam
determines the resolution for XLCT. In addition, no
background signal competes with NP luminescence (i.e., no
auto fluorescence) in XLCT. Currently, no small animal XLCT
system has been proposed or tested. This paper investigates
an XLCT system built and integrated with a dual source
micro-CT system. Two novel sampling paradigms that result in
more efficient scanning are proposed and tested via
simulations. Our preliminary experimental results in
phantoms indicate that a basic CT-like reconstruction is
able to recover a map of the NP locations and differences in
NP concentrations. With the proposed dual source system and
faster scanning approaches, XLCT has the potential to
revolutionize molecular imaging in preclinical
studies.},
Doi = {10.1117/12.911465},
Key = {fds268787}
}
@booklet{Maynor88,
Author = {MAYNOR, CH and CHARLES, HC and HERFKENS, RJ and SUDDARTH, SA and JOHNSON, GA},
Title = {INVITRO CHEMICAL-SHIFT IMAGING OF ATHEROSCLEROSIS AT
7.0-TESLA},
Journal = {INVESTIGATIVE RADIOLOGY},
Volume = {23},
Number = {9},
Pages = {S3-S3},
Publisher = {LIPPINCOTT-RAVEN PUBL},
Year = {1988},
Month = {September},
ISSN = {0020-9996},
url = {http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:A1988Q574500021&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=47d3190e77e5a3a53558812f597b0b92},
Key = {Maynor88}
}
@booklet{Thompson80,
Author = {Thompson, WM and Amberg, JR and Shaw, M},
Title = {Iodine concentration and radiographic density during oral
cholecystography},
Journal = {Investigative Radiology},
Volume = {15},
Number = {5},
Pages = {397},
Publisher = {Ovid Technologies (Wolters Kluwer Health)},
Year = {1980},
Month = {January},
ISSN = {0020-9996},
url = {http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:A1980KK17000048&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=47d3190e77e5a3a53558812f597b0b92},
Doi = {10.1097/00004424-198009000-00049},
Key = {Thompson80}
}
@booklet{Waldon89,
Author = {D. J. Waldon and A. E. Buhl and C. A. Baker and G. A.
Johnson},
Title = {Is minoxidil sulfate the active metabolite for
hair-growth},
Journal = {Clinical Research},
Volume = {37},
Number = {2},
Pages = {A769 -- A769},
Year = {1989},
Month = {April},
Key = {Waldon89}
}
@booklet{Waldon89a,
Author = {D. J. Waldon and A. E. Buhl and C. A. Baker and G. A.
Johnson},
Title = {Is minoxidil sulfate the active metabolite for
hair-growth},
Journal = {Journal Of Investigative Dermatology},
Volume = {92},
Number = {3},
Pages = {538 -- 538},
Year = {1989},
Month = {March},
Key = {Waldon89a}
}
@article{fds174206,
Author = {GA JOHNSON and RH MCCLUER},
Title = {ISOLATION AND ANALYSIS OF MONO-, DI-, AND
TRISIALOGANGLIOSIDES.},
Journal = {Biochimica et biophysica acta},
Volume = {70},
Pages = {487-90},
Year = {1963},
Month = {August},
ISSN = {0006-3002},
Keywords = {BRAIN* • CHROMATOGRAPHY* • EXPERIMENTAL LAB STUDY*
• GANGLIOSIDES* • HEXOSAMINES* • HEXOSES*
• NEURAMINIC ACIDS* • NEUROCHEMISTRY*},
Language = {eng},
Key = {fds174206}
}
@booklet{Wang00,
Author = {G. Y. Wang and G. A. Johnson and T. E. Spencer and F. W.
Bazer},
Title = {Isolation, immortalization, and initial characterization of
uterine cell lines: An in vitro model system for the porcine
uterus},
Journal = {In Vitro Cellular \& Developmental Biology-animal},
Volume = {36},
Number = {10},
Pages = {650 -- 656},
Year = {2000},
Key = {Wang00}
}
@article{fds174283,
Author = {G Wang and GA Johnson and TE Spencer and FW Bazer},
Title = {Isolation, immortalization, and initial characterization of
uterine cell lines: an in vitro model system for the porcine
uterus.},
Journal = {In vitro cellular & developmental biology.
Animal},
Volume = {36},
Number = {10},
Pages = {650-6},
Year = {2001},
Month = {May},
ISSN = {1071-2690},
Keywords = {Animals • Blotting, Western • Cell Division •
Cell Line, Transformed • Female • Models,
Biological* • Swine • Uterus •
cytology*},
Abstract = {The aim of this study was to develop immortalized cell lines
from porcine uterus. Endometrial cells including luminal
epithelium (LE), glandular epithelium (GE), stroma (ST), and
myometrium (MYO) were enzymatically isolated from the uterus
of a day 12 pregnant gilt. Primary cultures were
immortalized by transduction with a retroviral vector
containing the E6 and E7 open reading frames of human
papillomavirus type 16 (LXSN-16E6E7) packaged by the
amphotropic fibroblast line PA-317. Cells having integrated
the vector were selected by resistance to the neomycin
analog G418 (0.4-1.5 mg/ml). Surviving cells were maintained
in complete culture medium containing G418 (0.1 mg/ml) and
subcultured for 1 yr. Expression of the E7 protein was
confirmed in all cell lines by Western blotting. Phase
contrast microscopy revealed that LE and GE cells exhibited
cobblestone morphology, whereas ST and MYO cells exhibited
spindle-shaped morphology. The epithelial origin of LE and
GE was confirmed by positive immunostaining for cytokeratin.
Stromal and MYO cells were vimentin-positive, but
cytokeratin-negative. The MYO cell lines were positive for
smooth muscle alpha-actin staining, whereas LE, GE, and ST
cell lines were negative for alpha-actin. Western blotting
indicated that all cell lines expressed both estrogen and
progesterone receptors, but only GE cells secreted
uteroferrin (UF). Collectively, these porcine uterine cell
lines provide an in vitro model for studying cell
type-specific actions of hormones and cytokines, signal
transduction pathways, cell-cell interactions, and gene
expression.},
Language = {eng},
Key = {fds174283}
}
@article{fds132741,
Author = {G.A. Johnson},
Title = {J.R. MacFall, H.C.Charles, R.D. Black, H. Middleton,
J.Swartz, B. Saam, B. Dreihuys, C. Erickson, W. Happer,
G. Cates, G.A. Johnson, C.E. Ravin. Human lung air
spaces: potential for MR imaging with hyperpolarized He-3.
Radiology 200, 553-558. (1996).},
Year = {1996},
Key = {fds132741}
}
@article{fds132742,
Author = {G.A. Johnson},
Title = {J.R. MacFall, H.C.Charles, R.D. Black, H. Middleton,
J.Swartz, B. Saam, W. Happer, G. Cates, G.A. Johnson,
C.E. Ravin. MR imaging of lung air spaces with
hyperpolarized 3He. in "Proc., SMR 4th Annual Scientific
Meeting. New York, NY, 1996," p. 21.},
Year = {1996},
Key = {fds132742}
}
@article{fds132722,
Author = {G.A. Johnson},
Title = {J.S. MacFall, G.A. Johnson, in "Encyclopedia of Nuclear
Magnetic Resonance" (D.M. Grant, R.K. Harris, Ed.), p.
3633-3640, John Wiley & Sons, London, 1996.},
Year = {1996},
Key = {fds132722}
}
@article{fds132746,
Author = {G.A. Johnson},
Title = {Johnson GA, Hedlund LW, MacFall JR. A new window into the
lung. Physics in Medicine 35-38 1998},
Year = {1998},
Key = {fds132746}
}
@booklet{Ka01,
Author = {H. Ka and L. A. Jaeger and G. A. Johnson and T. E. Spencer and F. W. Bazer},
Title = {Keratinocyte growth factor is up-regulated by estrogen in
the porcine uterine endometrium and functions in
trophectoderm cell proliferation and differentiation},
Journal = {Endocrinology},
Volume = {142},
Number = {6},
Pages = {2303 -- 2310},
Year = {2001},
Month = {June},
Key = {Ka01}
}
@article{fds174110,
Author = {H Ka and LA Jaeger and GA Johnson and TE Spencer and FW
Bazer},
Title = {Keratinocyte growth factor is up-regulated by estrogen in
the porcine uterine endometrium and functions in
trophectoderm cell proliferation and differentiation.},
Journal = {Endocrinology},
Volume = {142},
Number = {6},
Pages = {2303-10},
Year = {2001},
Month = {June},
ISSN = {0013-7227},
Keywords = {Animals • Cell Differentiation • Cell Division
• Culture Techniques • Endometrium • Enzyme
Activation • Estradiol • Estrogens •
Estrogens, Catechol • Female • Fibroblast Growth
Factor 7 • Fibroblast Growth Factors • Gene
Expression Regulation • Gestational Age •
Mitogen-Activated Protein Kinase 1 • Mitogen-Activated
Protein Kinase 3 • Mitogen-Activated Protein Kinases
• Phosphorylation • Pregnancy • Progesterone
• Proliferating Cell Nuclear Antigen • Receptor,
Fibroblast Growth Factor, Type 2 • Receptors,
Fibroblast Growth Factor* • Receptors, Growth Factor
• Recombinant Proteins • Swine* •
Trophoblasts • analysis • cytology • drug
effects • genetics* • metabolism •
metabolism* • pharmacology • pharmacology*},
Abstract = {Keratinocyte growth factor (KGF) is expressed by uterine
endometrial epithelial cells during the estrous cycle and
during pregnancy in pigs, whereas KGF receptor is expressed
in conceptus trophectoderm and endometrial epithelia. In
particular, KGF expression in the endometrium is highest on
day 12 of pregnancy. This corresponds to the period of
maternal recognition of pregnancy in pigs, which is signaled
by large amounts of estrogen secreted by conceptus
trophectoderm acting on the endometrium. Our hypothesis is
that estrogens of conceptus origin stimulate endometrial
epithelial KGF expression, and, in turn, secreted KGF
stimulates proliferation and differentiation of conceptus
trophectoderm. To determine the factors affecting KGF
expression in the uterus, endometrial explants from gilts on
day 9 of the estrous cycle were cultured in the presence of
17beta-estradiol, catechol estrogens, or progesterone.
17beta-Estradiol stimulated the expression of KGF (P <
0.05), whereas catechol estrogens had no effect (P > 0.05).
Between days 9 and 15 of pregnancy, proliferating cell
nuclear antigen was abundant in conceptuses, but was barely
detectable in uterine endometrial epithelia. To determine
the effects of KGF on conceptus trophectoderm, porcine
trophectoderm (pTr) cells were treated with recombinant rat
KGF (rKGF). rKGF increased the proliferation of pTr cells (P
< 0.01) as measured by [(3)H]thymidine incorporation. rKGF
elicited phosphorylation of KGF receptor and activated the
mitogen-activated protein kinase (ERK1/2) cascade in pTr
cells. pTr cell differentiation was affected by rKGF,
because it increased expression of urokinase-type
plasminogen activator, a marker for differentiation in pTr
cells. Collectively, these results indicate that estrogen,
the pregnancy recognition signal from the conceptus in pigs,
increases uterine epithelial KGF expression, and, in turn,
KGF stimulates the proliferation and differentiation of
conceptus trophectoderm.},
Language = {eng},
Key = {fds174110}
}
@booklet{Ka00a,
Author = {H. Ka and T. E. Spencer and G. A. Johnson and F. W.
Bazer},
Title = {Keratinocyte growth factor: Expression by endometrial
epithelia of the porcine uterus},
Journal = {Biology Of Reproduction},
Volume = {62},
Number = {6},
Pages = {1772 -- 1778},
Year = {2000},
Month = {June},
Key = {Ka00a}
}
@article{fds174080,
Author = {H Ka and TE Spencer and GA Johnson and FW Bazer},
Title = {Keratinocyte growth factor: expression by endometrial
epithelia of the porcine uterus.},
Journal = {Biology of reproduction},
Volume = {62},
Number = {6},
Pages = {1772-8},
Year = {2000},
Month = {June},
ISSN = {0006-3363},
Keywords = {Animals • Blotting, Northern • Cloning, Molecular
• DNA, Complementary • Endometrium •
Epithelium • Estrus • Female • Fibroblast
Growth Factor 10 • Fibroblast Growth Factor 7 •
Fibroblast Growth Factors* • Gene Expression* •
Growth Substances • In Situ Hybridization •
Pregnancy • RNA, Messenger • Swine* •
analysis • genetics* • metabolism •
metabolism*},
Abstract = {Keratinocyte growth factor/fibroblast growth factor-7
(KGF/FGF-7) is an established paracrine mediator of
hormone-regulated epithelial growth and differentiation. In
all organs studied, KGF is uniquely expressed in cells of
mesenchymal origin. To determine whether KGF and its
receptor, keratinocyte growth factor receptor (KGFR) or
fibroblast growth factor receptor-2IIIb, were expressed in
the porcine uterus as a potential paracrine system mediating
progesterone action, we cloned KGF and KGFR partial cDNAs
from the porcine endometrium. KGF and KGFR expression was
detected in endometrium by Northern blot hybridization.
Interestingly, in situ hybridization results demonstrated
that KGF was expressed by endometrial epithelia and was
particularly abundant between Days 12 and 15 of the estrous
cycle and pregnancy. KGF secretion into the lumen of the
porcine uterus was also detected on Day 12 of the estrous
cycle and pregnancy. KGFR was expressed in both endometrial
epithelia and conceptus trophectoderm. These novel findings
suggest that KGF may act on the uterine endometrial
epithelium in an autocrine manner and on the conceptus
trophectoderm in a paracrine manner in the pig, which is the
only species possessing a true epitheliochorial type of
placentation.},
Language = {eng},
Key = {fds174080}
}
@article{fds132719,
Author = {G.A. Johnson},
Title = {L.W. Hedlund, M.D. Shattuck, G.A. Johnson.
Three-dimensional MR microscopy of pulmonary dynamics.
in "Proc., 1996. New York, NY, 1996,"
p.327.},
Year = {1996},
Key = {fds132719}
}
@article{fds132736,
Author = {G.A. Johnson},
Title = {L.W. Hedlund, S.L. Gewalt, G.P. Cofer, G.A. Johnson, in
"Application of Magnetic Resonance to the Study of the
Lung" (A. Cutillo, Ed.), p. 401-415, Futura Press, Mt.
Kisko, NY, 1996.},
Year = {1996},
Key = {fds132736}
}
@booklet{Shen-gunther98,
Author = {J. Shen-gunther and R. S. Mannel and J. L. Walker and G. A.
Johnson and A. E. Sienko},
Title = {Laparoscopic paraaortic lymphadenectomy using laparosonic
coagulating shears},
Journal = {Journal Of The American Association Of Gynecologic
Laparoscopists},
Volume = {5},
Number = {1},
Pages = {47 -- 50},
Year = {1998},
Month = {February},
Key = {Shen-gunther98}
}
@article{fds174302,
Author = {J Shen-Gunther and RS Mannel and JL Walker and GA Johnson and AE
Sienko},
Title = {Laparoscopic paraaortic lymphadenectomy using laparosonic
coagulating shears.},
Journal = {The Journal of the American Association of Gynecologic
Laparoscopists},
Volume = {5},
Number = {1},
Pages = {47-50},
Year = {1998},
Month = {February},
ISSN = {1074-3804},
Keywords = {Adult • Carcinoma, Squamous Cell • Female •
Hemostasis, Surgical • Humans • Laparoscopes*
• Laparoscopy • Lymph Node Excision • Middle
Aged • Surgical Instruments • Time Factors •
Ultrasonics • Uterine Cervical Neoplasms •
instrumentation • methods • methods* •
surgery},
Abstract = {With marked innovations in endosurgical instrumentation,
operative laparoscopy to include lymphadenectomy has become
feasible and has a valuable role in the management of
gynecologic malignancy. We used laparosonic coagulating
shears (LCS) for laparoscopic paraaortic lymphadenectomy in
two women with cervical carcinoma. Operating times for the
laparoscopic portion were 55 and 65 minutes and blood loss
was 20 and 30 ml, respectively. No surgical complications
were encountered. Lymphatic tissues were evaluated
histologically and no thermal artifacts were identified. The
major advantage of the ultrasonically activated scalpel of
the LCS is the ability to cut and coagulate tissues
simultaneously without electrical current. The LCS may
afford the surgeon a greater margin of safety than unipolar
electrocoagulation scissors by eliminating potential thermal
and electrical injury to vital structures.
Ultrasonic-activated technology deserves extended clinical
investigation in laparoscopic lymphadenectomy to
substantiate our preliminary findings, as well as to explore
its potential in gynecologic oncology.},
Language = {eng},
Key = {fds174302}
}
@booklet{Scribner02,
Author = {D. R. Scribner and J. L. Walker and G. A. Johnson and D. S.
Mcmeekin and M. A. Gold and R. S. Mannel},
Title = {Laparoscopic pelvic and paraaortic lymph node dissection in
the obese},
Journal = {Gynecologic Oncology},
Volume = {84},
Number = {3},
Pages = {426 -- 430},
Year = {2002},
Month = {March},
Key = {Scribner02}
}
@article{fds174296,
Author = {DR Scribner Jr and JL Walker and GA Johnson and DS McMeekin and MA Gold and RS Mannel},
Title = {Laparoscopic pelvic and paraaortic lymph node dissection in
the obese.},
Journal = {Gynecologic oncology},
Volume = {84},
Number = {3},
Pages = {426-30},
Year = {2002},
Month = {March},
ISSN = {0090-8258},
url = {http://dx.doi.org/10.1006/gyno.2001.6548},
Keywords = {Adult • Aged • Aged, 80 and over • Aorta,
Abdominal • Endometrial Neoplasms • Female •
Humans • Laparoscopy • Lymph Node Excision •
Lymphatic Metastasis • Middle Aged • Obesity
• Pelvis • Retrospective Studies •
complications • complications* • methods •
methods* • pathology • surgery*},
Abstract = {OBJECTIVE: The aim of this study was to determine the
utility of laparoscopic pelvic and paraaortic lymph node
dissection in obese women. METHODS: We performed a
retrospective analysis from 1/8/96 to 1/14/01 at the
University of Oklahoma Health Science Center, evaluating
patients who had a Quetelet index (QI) > or =28 and had
planned laparoscopic bilateral pelvic and paraaortic lymph
node dissections (lnd) for their gynecologic cancer. This
group was compared to a matched group of patients that had
lnd done by laparotomy. Patients were identified by our
institution's database and data were collected by review of
their medical records. Data were collected regarding
demographics, stage, histology, length of stay, and
procedural information including completion rates, estimated
blood loss (EBL), operating room (OR) time, lymph node
count, assistant, and complications. Associations between
variables were analyzed using Student t tests and chi(2)
testing, Excel v9.0. RESULTS: Fifty-five patients had
planned laparoscopic lnd (Group 1) and 45 patients had lnd
via laparotomy (Group 2). All patients had the diagnosis of
endometrial cancer. The percentage of stage I patients did
not differ between groups (42/55, 71.2% versus 37/45, 82.2%,
P = n.s.). Age and QI were also similar between groups,
(64.6 versus 58.4, 40.0 versus 39.3, P = n.s.). Laparoscopy
was completed in 35/55 (63.6%) cases. Reasons for conversion
included obesity (23.6%), adhesions (1.8%), intraperitoneal
cancer (5.5%), and bleeding (5.5%). QI > or =35 was
associated with a decreased success rate compared to QI <35
(44.4% versus 82.1%, P = 0.004). There was no difference in
successful laparoscopy when the first assistant was a fellow
or a community obstetrician/gynecologist (61.0% versus
50.0%, P = n.s.). The patients in Group 1 who had
laparoscopy completed had a longer OR time compared to those
in Group 2 (265.3 versus 140.7 min, P < 0.0001), EBL and
transfusion rates were equivalent (361.8 versus 344.2 ml,
5.6% versus 6.7%, P = n.s.), and length of stay was shorter
(2.8 versus 4.5 days, P = 0.0004). Group 1 had significantly
fewer postoperative fevers (5.5% versus 31.1%, P = 0.0007),
fewer postoperative ileus (0% versus 13.3%, P = 0.005), and
a trend for fewer wound infections (9.0% versus 22.2%, P =
0.07). CONCLUSIONS: Obesity is not a contraindication to
laparoscopic pelvic and paraaortic lymph node dissection.
The overall success rate was significantly higher in those
patients with a QI <35. Advantages include shorter hospital
stay, fewer postoperative fevers, fewer postoperative ileus,
and possibly fewer wound infections.},
Language = {eng},
Doi = {10.1006/gyno.2001.6548},
Key = {fds174296}
}
@booklet{Scribner01a,
Author = {D. R. Scribner and J. L. Walker and G. A. Johnson and S. D.
Mcmeekin and M. A. Gold and R. S. Mannel},
Title = {Laparoscopic pelvic and paraaortic lymph node dissection:
Analysis of the first 100 cases},
Journal = {Gynecologic Oncology},
Volume = {82},
Number = {3},
Pages = {498 -- 503},
Year = {2001},
Month = {September},
Key = {Scribner01a}
}
@article{fds174212,
Author = {DR Scribner Jr and JL Walker and GA Johnson and SD McMeekin and MA Gold and RS Mannel},
Title = {Laparoscopic pelvic and paraaortic lymph node dissection:
analysis of the first 100 cases.},
Journal = {Gynecologic oncology},
Volume = {82},
Number = {3},
Pages = {498-503},
Year = {2001},
Month = {September},
ISSN = {0090-8258},
url = {http://dx.doi.org/10.1006/gyno.2001.6314},
Keywords = {Adult • Aged • Aged, 80 and over • Aorta,
Thoracic • Endometrial Neoplasms • Female •
Humans • Laparoscopy • Lymph Node Excision •
Middle Aged • Neoplasm Staging • Ovarian Neoplasms
• Pelvis • Retrospective Studies • adverse
effects • methods* • pathology •
surgery*},
Abstract = {OBJECTIVE: The aim of this study was to analyze the first
100 cases of planned laparoscopic pelvic and paraaortic
lymph node dissection (LND) done for staging of gynecologic
cancers. The goal of the study was to assess prognostic
factors for conversion to laparotomy and document
complications. METHODS: A retrospective review of patients
who had planned laparoscopic bilateral pelvic and bilateral
paraaortic LND for staging of their gynecologic cancer was
performed. Patients were identified by our institutional
database and data were collected by review of their medical
records. Data were obtained regarding demographics, stage,
histology, length of stay, and procedural information
including completion rates, operating room time, estimated
blood loss, assistant, lymph node count, and complications.
Associations between variables were analyzed using Student t
tests, analysis of variance, and chi(2) testing (Excel
v7.0). RESULTS: A total of 103 patients were identified from
12/15/95 to 8/28/00. Demographics included mean age of 66.2
(25-92) and mean Quetelet index (QI) of 30.8 (15.9-56.1). A
total of 34/103 (33.0%) had > or =1 previous laparotomy.
Ninety-five patients had endometrial cancer and 8 had
ovarian cancer. Eighty-six of 103 (83.5%) were stage I or
II. The length of stay was shorter for those who had
laparoscopy than for those who needed conversion to
laparotomy (2.8 vs 5.6 days, P < 0.0001). Laparoscopy was
completed in 73/103 (70.9%) of the cases. Completion rates
were 62/76 (81.6%) with QI < 35 vs 11/27 (40.7%) with QI >
or = 35, P < 0.001. Significantly more patients had their
laparoscopy completed when an attending gynecologic
oncologist was the first assistant compared to a fellow or a
community obstetrician/gynecologist (92.9%, 69.0%, 64.5%, P
< 0.0001). The top three reasons for conversion to
laparotomy were obesity, 12/30 (29.1%), adhesions, 5/30
(16.7%), and intraperitoneal disease, 5/30 (16.7%). Pelvic,
common iliac, and paraaortic lymph node counts did not
differ when compared to those of patients who had conversion
to laparotomy (18.1, 5.1, 6.8 vs 17.3, 5.7, 6.8, P = ns).
Complications included 2 urinary tract injuries, 2 pulmonary
embolisms, and 6 wound infections (all in the laparotomy
group). Two deaths occurred, 1 due to a vascular injury on
initial trocar insertion and 1 due to a pulmonary embolism
after a laparotomy for bowel herniation through a trocar
incision. CONCLUSION: Laparoscopic bilateral pelvic and
paraaortic LND can be completed successfully in 70.9% of
patients. Age, obesity, previous surgery, and the need to
perform this procedure in the community were not
contraindications. Advantages include a shorter hospital
stay, similar nodal counts, and acceptable
complications.},
Language = {eng},
Doi = {10.1006/gyno.2001.6314},
Key = {fds174212}
}
@article{fds268842,
Author = {Song, J and Liu, Y and Gewalt, SL and Cofer, G and Johnson, GA and Liu,
QH},
Title = {Least-square NUFFT methods applied to 2-D and 3-D radially
encoded MR image reconstruction.},
Journal = {IEEE Trans Biomed Eng},
Volume = {56},
Number = {4},
Pages = {1134-1142},
Year = {2009},
Month = {April},
ISSN = {1558-2531},
url = {http://www.ncbi.nlm.nih.gov/pubmed/19174334},
Keywords = {Animals • Computer Simulation • Image Processing,
Computer-Assisted • Least-Squares Analysis* •
Magnetic Resonance Imaging • Mice • Models,
Statistical • Phantoms, Imaging •
methods*},
Abstract = {Radially encoded MRI has gained increasing attention due to
its motion insensitivity and reduced artifacts. However,
because its samples are collected nonuniformly in the
k-space, multidimensional (especially 3-D) radially sampled
MRI image reconstruction is challenging. The objective of
this paper is to develop a reconstruction technique in high
dimensions with on-the-fly kernel calculation. It implements
general multidimensional nonuniform fast Fourier transform
(NUFFT) algorithms and incorporates them into a k-space
image reconstruction framework. The method is then applied
to reconstruct from the radially encoded k-space data,
although the method is applicable to any non-Cartesian
patterns. Performance comparisons are made against the
conventional Kaiser-Bessel (KB) gridding method for 2-D and
3-D radially encoded computer-simulated phantoms and
physically scanned phantoms. The results show that the NUFFT
reconstruction method has better accuracy-efficiency
tradeoff than the KB gridding method when the kernel weights
are calculated on the fly. It is found that for a particular
conventional kernel function, using its corresponding
deapodization function as a scaling factor in the NUFFT
framework has the potential to improve accuracy. In
particular, when a cosine scaling factor is used, the NUFFT
method is faster than KB gridding method since a closed-form
solution is available and is less computationally expensive
than the KB kernel (KB griding requires computation of
Bessel functions). The NUFFT method has been successfully
applied to 2-D and 3-D in vivo studies on small
animals.},
Doi = {10.1109/TBME.2009.2012721},
Key = {fds268842}
}
@article{fds268861,
Author = {Badea, CT and Wetzel, AW and Mistry, N and Pomerantz, S and Nave, D and Johnson, GA},
Title = {Left ventricle volume measurements in cardiac micro-CT: the
impact of radiation dose and contrast agent.},
Journal = {Comput Med Imaging Graph},
Volume = {32},
Number = {3},
Pages = {239-250},
Year = {2008},
Month = {April},
ISSN = {0895-6111},
url = {http://www.ncbi.nlm.nih.gov/pubmed/18243656},
Keywords = {Animals • Contrast Media • Heart Ventricles •
Imaging, Three-Dimensional • Mice • Mice, Inbred
C57BL • Radiation Dosage • Radiographic Image
Interpretation, Computer-Assisted • Tomography, X-Ray
Computed • administration & dosage • methods*
• radiography*},
Abstract = {Micro-CT-based cardiac function estimation in small animals
requires measurement of left ventricle (LV) volume at
multiple time points during the cardiac cycle. Measurement
accuracy depends on the image resolution, its signal and
noise properties, and the analysis procedure. This work
compares the accuracy of the Otsu thresholding and a region
sampled binary mixture approach, for live mouse LV volume
measurement using 100 microm resolution datasets. We
evaluate both analysis methods after varying the volume of
injected contrast agent and the number of projections used
for CT reconstruction with a goal of permitting reduced
levels of both X-ray and contrast agent doses.},
Doi = {10.1016/j.compmedimag.2007.12.004},
Key = {fds268861}
}
@article{fds292761,
Author = {Spielmann, AL and Nelson, RC and Lowry, CR and Johnson, GA and Sundaramoothy, G and Sheafor, DH and Paulson, EK},
Title = {Liver: single breath-hold dynamic subtraction CT with
multi-detector row helical technology feasibility
study.},
Journal = {Radiology},
Volume = {222},
Number = {1},
Pages = {278-283},
Year = {2002},
Month = {January},
ISSN = {0033-8419},
url = {http://www.ncbi.nlm.nih.gov/pubmed/11756737},
Keywords = {Adult • Aged • Feasibility Studies • Female
• Humans • Liver Neoplasms • Male •
Middle Aged • Neovascularization, Pathologic •
Prospective Studies • Subtraction Technique •
Tomography, X-Ray Computed • methods* •
radiography • radiography* • secondary},
Abstract = {Fifty-two patients with known or suspected hypervascular
malignancy were examined to determine the technical
feasibility of performing single-breath-hold dynamic
subtraction computed tomography (CT) of the liver with
multi-detector row helical CT. The precontrast and hepatic
arterial CT scans, which were acquired during the same
breath hold, were subtracted. The mean liver-to-muscle
contrast ratio on the precontrast, hepatic arterial, and
subtracted images was 1.3, 1.4, and 2.3, respectively. In 13
patients with lesions, the subtracted images showed a
2.5-fold increase in mean lesion contrast compared with the
hepatic arterial CT scans.},
Language = {eng},
Doi = {10.1148/radiol.2221010190},
Key = {fds292761}
}
@article{fds268694,
Author = {Borg, JS and Vu, M-A and Badea, C and Badea, A and Johnson, GA and Dzirasa,
K},
Title = {Localization of Metal Electrodes in the Intact Rat Brain
Using Registration of 3D Microcomputed Tomography Images to
a Magnetic Resonance Histology Atlas.},
Journal = {eNeuro},
Volume = {2},
Number = {4},
Pages = {e0017},
Year = {2015},
url = {http://hdl.handle.net/10161/10327 Duke open
access},
Abstract = {Simultaneous neural recordings taken from multiple areas of
the rodent brain are garnering growing interest due to the
insight they can provide about spatially distributed neural
circuitry. The promise of such recordings has inspired great
progress in methods for surgically implanting large numbers
of metal electrodes into intact rodent brains. However,
methods for localizing the precise location of these
electrodes have remained severely lacking. Traditional
histological techniques that require slicing and staining of
physical brain tissue are cumbersome, and become
increasingly impractical as the number of implanted
electrodes increases. Here we solve these problems by
describing a method that registers 3-D computerized
tomography (CT) images of intact rat brains implanted with
metal electrode bundles to a Magnetic Resonance Imaging
Histology (MRH) Atlas. Our method allows accurate
visualization of each electrode bundle's trajectory and
location without removing the electrodes from the brain or
surgically implanting external markers. In addition, unlike
physical brain slices, once the 3D images of the electrode
bundles and the MRH atlas are registered, it is possible to
verify electrode placements from many angles by "re-slicing"
the images along different planes of view. Further, our
method can be fully automated and easily scaled to
applications with large numbers of specimens. Our digital
imaging approach to efficiently localizing metal electrodes
offers a substantial addition to currently available
methods, which, in turn, may help accelerate the rate at
which insights are gleaned from rodent network
neuroscience.},
Doi = {10.1523/ENEURO.0017-15.2015},
Key = {fds268694}
}
@booklet{Ford82a,
Author = {Ford, KK and Heinz, ER and Johnson, GA and Drayer, BP and Dubois,
PJ},
Title = {Low-cost digital subtraction},
Journal = {American Journal Of Neuroradiology},
Volume = {3},
Number = {1},
Pages = {99-99},
Year = {1982},
Key = {Ford82a}
}
@booklet{Ford82,
Author = {Ford, KK and Heinz, ER and Johnson, GA and Drayer, BD and Dubois,
PJ},
Title = {Low-cost digital subtraction angiography.},
Journal = {AJNR Am J Neuroradiol},
Volume = {3},
Number = {4},
Pages = {448-451},
Year = {1982},
ISSN = {0195-6108},
url = {http://www.ncbi.nlm.nih.gov/pubmed/6810680},
Key = {Ford82}
}
@article{fds132892,
Author = {KK Ford and ER Heinz and GA Johnson and BD Drayer and PJ
Dubois},
Title = {Low-cost digital subtraction angiography.},
Journal = {AJNR. American journal of neuroradiology, UNITED
STATES},
Volume = {3},
Number = {4},
Pages = {448-51},
ISSN = {0195-6108},
Keywords = {Angiography • Humans • Radiographic Image
Enhancement • Subtraction Technique • Videotape
Recording • economics • methods*},
Key = {fds132892}
}
@article{fds268830,
Author = {Badea, CT and Johnston, SM and Subashi, E and Qi, Y and Hedlund, LW and Johnson, GA},
Title = {Lung perfusion imaging in small animals using 4D micro-CT at
heartbeat temporal resolution.},
Journal = {Med Phys},
Volume = {37},
Number = {1},
Pages = {54-62},
Year = {2010},
Month = {January},
ISSN = {0094-2405},
url = {http://www.ncbi.nlm.nih.gov/pubmed/20175466},
Keywords = {Animals • Cardiac-Gated Imaging Techniques •
Contrast Media • Iopamidol • Lung •
Microinjections • Perfusion Imaging • Radiographic
Image Enhancement • Rats • Rats, Inbred F344
• Reproducibility of Results • Sensitivity and
Specificity • Tomography, X-Ray Computed •
administration & dosage • diagnostic use* •
methods • methods* • radiography* •
veterinary • veterinary*},
Abstract = {PURPOSE: Quantitative in vivo imaging of lung perfusion in
rodents can provide critical information for preclinical
studies. However, the combined challenges of high temporal
and spatial resolution have made routine quantitative
perfusion imaging difficult in small animals. The purpose of
this work is to demonstrate 4D micro-CT for perfusion
imaging in rodents at heartbeat temporal resolution and
isotropic spatial resolution. METHODS: We have recently
developed a dual tube/detector micro-CT scanner that is well
suited to capture first pass kinetics of a bolus of contrast
agent used to compute perfusion information. Our approach is
based on the paradigm that similar time density curves can
be reproduced in a number of consecutive, small volume
injections of iodinated contrast agent at a series of
different angles. This reproducibility is ensured by the
high-level integration of the imaging components of our
system with a microinjector, a mechanical ventilator, and
monitoring applications. Sampling is controlled through a
biological pulse sequence implemented in LABVIEW. Image
reconstruction is based on a simultaneous algebraic
reconstruction technique implemented on a graphic processor
unit. The capabilities of 4D micro-CT imaging are
demonstrated in studies on lung perfusion in rats. RESULTS:
We report 4D micro-CT imaging in the rat lung with a
heartbeat temporal resolution (approximately 150 ms) and
isotropic 3D reconstruction with a voxel size of 88 microm
based on sampling using 16 injections of 50 microL each. The
total volume of contrast agent injected during the
experiments (0.8 mL) was less than 10% of the total blood
volume in a rat. This volume was not injected in a single
bolus, but in multiple injections separated by at least 2
min interval to allow for clearance and adaptation. We
assessed the reproducibility of the time density curves with
multiple injections and found that these are very similar.
The average time density curves for the first eight and last
eight injections are slightly different, i.e., for the last
eight injections, both the maximum of the average time
density curves and its area under the curve are decreased by
3.8% and 7.2%, respectively, relative to the average time
density curves based on the first eight injections. The
radiation dose associated with our 4D micro-CT imaging is
0.16 Gy and is therefore in the range of a typical micro-CT
dose. CONCLUSIONS: 4D micro-CT-based perfusion imaging
demonstrated here has immediate application in a wide range
of preclinical studies such as tumor perfusion,
angiogenesis, and renal function. Although our imaging
system is in many ways unique, we believe that our approach
based on the multiple injection paradigm can be used with
the newly developed flat-panel slip-ring-based micro-CT to
increase their temporal resolution in dynamic perfusion
studies.},
Language = {eng},
Doi = {10.1118/1.3264619},
Key = {fds268830}
}
@article{fds132734,
Author = {G.A. Johnson},
Title = {M. Delnomdedieu, L. W. Hedlund, R.R. Maronpot, G.A. Johnson.
MR microscopy to follow bromobenzene-induced hepatoxicity
in the rat. in "Proc., SMR 4th Annual Scientific Meeting.
New York, NY, 1996," p.325.},
Year = {1996},
Key = {fds132734}
}
@article{fds132733,
Author = {G.A. Johnson},
Title = {M. Delnomdedieu, L.W. Hedlund, G.A. Johnson, R.R. Maronpot,
Magnetic Resonance Microscopy-A New Tool for the
Toxicologic Pathologist. Toxicologic Pathology 24, 36-44
(1996).},
Year = {1996},
Key = {fds132733}
}
@article{fds132723,
Author = {G.A. Johnson},
Title = {M.D. Shattuck, G.P. Cofer, G.H. Glover, L.W. Hedlund, G.A.
Johnson. Three-dimensional projection microscopy of the
lung. in "Proc., SMR 4th Scientific Meeting. New York,
NY, 1996," p. 18.},
Year = {1996},
Key = {fds132723}
}
@article{fds268750,
Author = {Sulik, KK and O'Leary-Moore, SK and Parnell, SE and Lipinski, RJ and Pecevich, S and Holloway, HT and Ament, J and Oguz, I and Budin, F and Jiang, Y and Dehart, DB and Styner, MA and Johnson,
GA},
Title = {Magnetic resonance and diffusion tensor imaging of pre- and
postnatal brains in a mouse Fetal Alcohol Spectrum Disorders
model},
Journal = {ALCOHOL},
Volume = {45},
Number = {3},
Pages = {282-282},
Publisher = {ELSEVIER SCIENCE INC},
Year = {2011},
Month = {May},
ISSN = {0741-8329},
url = {http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000289538300070&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=47d3190e77e5a3a53558812f597b0b92},
Key = {fds268750}
}
@article{fds269032,
Author = {Möller, HE and Chawla, MS and Chen, XJ and Driehuys, B and Hedlund, LW and Wheeler, CT and Johnson, GA},
Title = {Magnetic resonance angiography with hyperpolarized 129Xe
dissolved in a lipid emulsion.},
Journal = {Magn Reson Med},
Volume = {41},
Number = {5},
Pages = {1058-1064},
Year = {1999},
Month = {May},
ISSN = {0740-3194},
url = {http://www.ncbi.nlm.nih.gov/pubmed/10332890},
Keywords = {Abdomen • Animals • Artifacts • Blood Flow
Velocity • Blood Volume • Contrast Media* •
Electron Spin Resonance Spectroscopy • Fat Emulsions,
Intravenous • Iliac Vein • Injections, Intravenous
• Lasers • Magnetic Resonance Angiography •
Magnetic Resonance Spectroscopy • Male • Pelvis
• Rats • Renal Veins • Signal Processing,
Computer-Assisted • Veins • Vena Cava, Inferior
• Xenon Isotopes* • administration & dosage •
anatomy & histology • blood supply • diagnostic
use* • methods*},
Abstract = {Hyperpolarized (HP) 129Xe can be dissolved in biologically
compatible lipid emulsions while maintaining sufficient
polarization for in vivo vascular imaging. For xenon in
Intralipid 30%, in vitro spectroscopy at 2 T yielded a
chemical shift of 197 +/- 1 ppm with reference to xenon gas,
a spin-lattice relaxation time T1 = 25.3 +/- 2.1 sec, and a
T2* time constant of 37 +/- 5 msec. Angiograms of the
abdominal and pelvic veins in the rat obtained with 129Xe
MRI after intravenous injection of HP 129Xe/Intralipid 30%
into the tail demonstrated signal-to-noise ratios between 8
and 29. An analysis of the inflow effect on time-of-flight
images of two segments of the inferior vena cava yielded
additional information. The mean blood flow velocity was
34.7 +/- 1.0 mm/sec between the junction of the caudal veins
and the kidneys and 13.3 +/- 0.8 mm/sec at the position of
the diaphragm. The mean volume flow rates in these segments
were 7.2 +/- 3.4 ml/min and 11.0 +/- 2.8 ml/min,
respectively. Intravenous delivery of HP 129Xe dissolved in
a carrier may lead to novel biomedical applications of
laser-polarized gases.},
Doi = {10.1002/(sici)1522-2594(199905)41:5<1058::aid-mrm26>3.0.co;2},
Key = {fds269032}
}
@booklet{Moller99a,
Author = {H. E. Moller and M. S. Chawla and X. J. Chen and B. Driehuys and L. W. Hedlund and C. T. Wheeler and G. A.
Johnson},
Title = {Magnetic resonance angiography with hyperpolarized Xe-129
dissolved in a lipid emulsion},
Journal = {Magnetic Resonance In Medicine},
Volume = {41},
Number = {5},
Pages = {1058 -- 1064},
Year = {1999},
Month = {May},
Key = {Moller99a}
}
@article{fds132780,
Author = {GA Johnson and GP Cofer and B Fubara and SL Gewalt and LW Hedlund and RR
Maronpot},
Title = {Magnetic resonance histology for morphologic
phenotyping.},
Journal = {Journal of magnetic resonance imaging : JMRI, United
States},
Volume = {16},
Number = {4},
Pages = {423-9},
Year = {2002},
Month = {October},
ISSN = {1053-1807},
Keywords = {Animals • Magnetic Resonance Imaging* • Mice
• Mice, Inbred C57BL • Phenotype •
methods},
Abstract = {Magnetic resonance histology (MRH) images of the whole mouse
have been acquired at 100-micron isotropic resolution at 2.0
T with image arrays of 256 x 256 x 1024. Higher resolution
(50 x 50 x 50 microns) of limited volumes has been acquired
at 7.1T with image arrays of 512 x 512 x 512. Even higher
resolution images (20 x 20 x 20 microns) of isolated organs
have been acquired at 9.4 T. The volume resolution
represents an increase of 625000 x over conventional
clinical MRI. The technological basis is summarized that
will allow basic scientists to begin using MRH as a routine
method for morphologcic phenotyping of the mouse. MRH
promises four unique attributes over conventional histology:
1). MRH is non-destructive; 2). MRH exploits the unique
contrast mechanisms that have made MRI so successful
clinically; 3). MRH is 3-dimensional; and 4). the data are
inherently digital. We demonstrate the utility in
morphologic phenotyping a whole C57BL/6J
mouse.},
Key = {fds132780}
}
@booklet{Johnson02a,
Author = {Johnson, GA and Cofer, GP and Fubara, B and Gewalt, SL and Hedlund, LW and Maronpot, RR},
Title = {Magnetic resonance histology for morphologic
phenotyping.},
Journal = {J Magn Reson Imaging},
Volume = {16},
Number = {4},
Pages = {423-429},
Year = {2002},
Month = {October},
ISSN = {1053-1807},
url = {http://www.ncbi.nlm.nih.gov/pubmed/12353257},
Abstract = {Magnetic resonance histology (MRH) images of the whole mouse
have been acquired at 100-micron isotropic resolution at 2.0
T with image arrays of 256 x 256 x 1024. Higher resolution
(50 x 50 x 50 microns) of limited volumes has been acquired
at 7.1T with image arrays of 512 x 512 x 512. Even higher
resolution images (20 x 20 x 20 microns) of isolated organs
have been acquired at 9.4 T. The volume resolution
represents an increase of 625000 x over conventional
clinical MRI. The technological basis is summarized that
will allow basic scientists to begin using MRH as a routine
method for morphologcic phenotyping of the mouse. MRH
promises four unique attributes over conventional histology:
1). MRH is non-destructive; 2). MRH exploits the unique
contrast mechanisms that have made MRI so successful
clinically; 3). MRH is 3-dimensional; and 4). the data are
inherently digital. We demonstrate the utility in
morphologic phenotyping a whole C57BL/6J
mouse.},
Doi = {10.1002/jmri.10175},
Key = {Johnson02a}
}
@article{fds268773,
Author = {Xie, L and Cianciolo, RE and Hulette, B and Lee, HW and Qi, Y and Cofer, G and Johnson, GA},
Title = {Magnetic resonance histology of age-related nephropathy in
the Sprague Dawley rat.},
Journal = {Toxicol Pathol},
Volume = {40},
Number = {5},
Pages = {764-778},
Year = {2012},
Month = {July},
url = {http://www.ncbi.nlm.nih.gov/pubmed/22504322},
Abstract = {Magnetic resonance histology (MRH) has become a valuable
tool in evaluating drug-induced toxicity in preclinical
models. However, its application in renal injury has been
limited. This study tested the hypothesis that MRH could
detect image-based biomarkers of chronic disease,
inflammation, or age-related degeneration in the kidney,
laying the foundation for more extensive use in evaluating
drug toxicity. We examined the entire intact kidney in a
spontaneous model of chronic progressive nephropathy.
Kidneys from male Sprague Dawley rats were imaged at 8 weeks
(n = 4) and 52 weeks (n =4) on a 9.4 T system dedicated to
MR microscopy. Several potential contrast mechanisms were
explored to optimize the scanning protocols. Full coverage
of the entire kidney was achieved with isotropic spatial
resolution at 31 microns (voxel volume = 30 pL) using a
gradient recalled echo sequence. Isotropic spatial
resolution of 15 microns (voxel volume < 4 pL) was achieved
in a biopsy core specimen. Qualitative age-related
structural changes, such as renal cortical microvasculature,
tubular dilation, interstitial fibrosis, and glomerular
architecture, were apparent. The nondestructive 3D images
allowed measurement of quantitative differences of kidney
volume, pelvis volume, main vessel volume, glomerular size,
as well as thickness of the cortex, outer medulla, and inner
medulla.},
Doi = {10.1177/0192623312441408},
Key = {fds268773}
}
@article{fds268706,
Author = {Johnson, GA},
Title = {Magnetic resonance histology.},
Journal = {J Magn Reson Imaging},
Volume = {42},
Number = {1},
Pages = {1-2},
Year = {2015},
Month = {July},
ISSN = {1053-1807},
url = {http://dx.doi.org/10.1002/jmri.24774},
Doi = {10.1002/jmri.24774},
Key = {fds268706}
}
@article{fds268728,
Author = {Johnson, GA and Badea, A and Calabrese, E and Liu, C and Xie,
L},
Title = {Magnetic resonance histology: cool images- but who
cares?},
Journal = {TOXICOLOGY LETTERS},
Volume = {221},
Pages = {S50-S50},
Publisher = {Elsevier BV},
Year = {2013},
ISSN = {0378-4274},
url = {http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000323865800154&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=47d3190e77e5a3a53558812f597b0b92},
Doi = {10.1016/j.toxlet.2013.06.183},
Key = {fds268728}
}
@article{fds268705,
Author = {Johnson, GA and Badea, A and Calabrese, E and Liu, C and Xie,
L},
Title = {Magnetic resonance histology—Applications in
toxicology},
Journal = {Toxicology Letters},
Volume = {229},
Pages = {S31-S32},
Publisher = {Elsevier BV},
Year = {2014},
Month = {September},
ISSN = {0378-4274},
url = {http://dx.doi.org/10.1016/j.toxlet.2014.06.148},
Doi = {10.1016/j.toxlet.2014.06.148},
Key = {fds268705}
}
@booklet{Dixon88,
Author = {Dixon, D and Johnson, GA and Cofer, GP and Hedlund, LW and Maronpot,
RR},
Title = {Magnetic resonance imaging (MRI): a new tool in experimental
toxicologic pathology.},
Journal = {Toxicol Pathol},
Volume = {16},
Number = {3},
Pages = {386-391},
Year = {1988},
ISSN = {0192-6233},
url = {http://www.ncbi.nlm.nih.gov/pubmed/3194661},
Abstract = {Magnetic Resonance Imaging (MRI) is a noninvasive imaging
technique that provides multidimensional images of the soft
tissues of the body. This imaging technique has proven to be
an excellent diagnostic and experimental tool for the
detection of pathologic alterations in soft tissues, as well
as an adjunct screening method for following the genesis,
progression, or regression of chemically induced lesions in
the same live animal. Future applications of MRI technology
in small animals include MRI microscopy, mapping of vascular
or circulatory alterations, measurement of perfusion and
diffusion rates of body fluids, and acquisition of cell
metabolic states in combination with Nuclear Magnetic
Resonance (NMR) spectroscopy, all of which will contribute
immensely to the advancement of toxicologic and biomolecular
research.},
Doi = {10.1177/019262338801600311},
Key = {Dixon88}
}
@article{fds132761,
Author = {D Dixon and GA Johnson and GP Cofer and LW Hedlund and RR
Maronpot},
Title = {Magnetic resonance imaging (MRI): a new tool in experimental
toxicologic pathology.},
Journal = {Toxicologic pathology, UNITED STATES},
Volume = {16},
Number = {3},
Pages = {386-91},
Year = {1988},
ISSN = {0192-6233},
Keywords = {Animals • Magnetic Resonance Imaging* • Pathology*
• Rats • Toxicology*},
Abstract = {Magnetic Resonance Imaging (MRI) is a noninvasive imaging
technique that provides multidimensional images of the soft
tissues of the body. This imaging technique has proven to be
an excellent diagnostic and experimental tool for the
detection of pathologic alterations in soft tissues, as well
as an adjunct screening method for following the genesis,
progression, or regression of chemically induced lesions in
the same live animal. Future applications of MRI technology
in small animals include MRI microscopy, mapping of vascular
or circulatory alterations, measurement of perfusion and
diffusion rates of body fluids, and acquisition of cell
metabolic states in combination with Nuclear Magnetic
Resonance (NMR) spectroscopy, all of which will contribute
immensely to the advancement of toxicologic and biomolecular
research.},
Key = {fds132761}
}
@article{fds268893,
Author = {Herfkens, RJ and Johnson, GA},
Title = {Magnetic resonance imaging at high-strength magnetic
fields.},
Journal = {Magn Reson Annu},
Pages = {197-215},
Year = {1985},
ISSN = {8756-9787},
url = {http://www.ncbi.nlm.nih.gov/pubmed/3917235},
Keywords = {Animals • Brain • Electromagnetic Fields •
Heart • Humans • Magnetic Resonance Imaging •
Magnetic Resonance Spectroscopy • anatomy & histology
• instrumentation • methods*},
Key = {fds268893}
}
@article{fds268898,
Author = {Cyr, M and Caron, MG and Johnson, GA and Laakso, A},
Title = {Magnetic resonance imaging at microscopic resolution reveals
subtle morphological changes in a mouse model of
dopaminergic hyperfunction.},
Journal = {Neuroimage},
Volume = {26},
Number = {1},
Pages = {83-90},
Year = {2005},
Month = {May},
ISSN = {1053-8119},
url = {http://www.ncbi.nlm.nih.gov/pubmed/15862208},
Abstract = {Structural abnormalities of the basal ganglia have been
documented in several neuropsychiatric conditions associated
with dysregulation of the dopamine system. However, the
histological nature underlying these changes is largely
unknown. Using magnetic resonance imaging at microscopic
resolution (MRI, 9.4 T with 43 microm isotropic spatial
resolution) and stereological techniques, we have
investigated the effect of increased dopamine
neurotransmission on brain morphology in mice with elevated
extracellular dopamine, the dopamine transporter knockout
(DAT-KO) mice. We first demonstrate the usefulness of MRI at
microscopic resolution for the accurate identification and
measurement of volumes of specific subregions, accounting
for less than 0.03% (0.16 mm(3)) of the volume of a mouse
brain. Furthermore, the MRI analysis reveals a significantly
lower volume (-9%) of the anterior striatum of DAT-KO mice,
while the volume of other dopamine-related structures such
as the posterior striatum and the substantia nigra pars
reticulata is unchanged in comparison to wild type
littermates. Stereological analysis performed in the same
brains reveals that one important structural factor
accounting for this selective change in volume is a
reduction of 18% in the absolute number of neuronal cell
bodies. The feasibility of assessing accurately small
morphological alterations in mouse models, where the
molecular and histological pathologies can be easily
compared in a controlled manner, provides a paradigm to
examine the relevance of selective brain volumetric changes
associated with a number of neuropathological
conditions.},
Doi = {10.1016/j.neuroimage.2005.01.039},
Key = {fds268898}
}
@article{fds132905,
Author = {M Cyr and MG Caron and GA Johnson and A Laakso},
Title = {Magnetic resonance imaging at microscopic resolution reveals
subtle morphological changes in a mouse model of
dopaminergic hyperfunction.},
Journal = {NeuroImage, United States},
Volume = {26},
Number = {1},
Pages = {83-90},
Year = {2005},
Month = {May},
ISSN = {1053-8119},
Keywords = {Animals • Basal Ganglia • Brain • Cell Count
• Dopamine • Dopamine Plasma Membrane Transport
Proteins • Fluorescent Antibody Technique • Image
Interpretation, Computer-Assisted • Magnetic Resonance
Imaging • Male • Membrane Glycoproteins •
Membrane Transport Proteins • Mice • Mice,
Knockout • Models, Neurological • Neostriatum
• Nerve Tissue Proteins • Neurons • anatomy &
histology • anatomy & histology* • cytology •
genetics • physiology • physiology*},
Abstract = {Structural abnormalities of the basal ganglia have been
documented in several neuropsychiatric conditions associated
with dysregulation of the dopamine system. However, the
histological nature underlying these changes is largely
unknown. Using magnetic resonance imaging at microscopic
resolution (MRI, 9.4 T with 43 microm isotropic spatial
resolution) and stereological techniques, we have
investigated the effect of increased dopamine
neurotransmission on brain morphology in mice with elevated
extracellular dopamine, the dopamine transporter knockout
(DAT-KO) mice. We first demonstrate the usefulness of MRI at
microscopic resolution for the accurate identification and
measurement of volumes of specific subregions, accounting
for less than 0.03% (0.16 mm(3)) of the volume of a mouse
brain. Furthermore, the MRI analysis reveals a significantly
lower volume (-9%) of the anterior striatum of DAT-KO mice,
while the volume of other dopamine-related structures such
as the posterior striatum and the substantia nigra pars
reticulata is unchanged in comparison to wild type
littermates. Stereological analysis performed in the same
brains reveals that one important structural factor
accounting for this selective change in volume is a
reduction of 18% in the absolute number of neuronal cell
bodies. The feasibility of assessing accurately small
morphological alterations in mouse models, where the
molecular and histological pathologies can be easily
compared in a controlled manner, provides a paradigm to
examine the relevance of selective brain volumetric changes
associated with a number of neuropathological
conditions.},
Key = {fds132905}
}
@article{fds132879,
Author = {BP Drayer and P Burger and B Hurwitz and D Dawson and J Cain and J Leong and R
Herfkens, GA Johnson},
Title = {Magnetic resonance imaging in multiple sclerosis: decreased
signal in thalamus and putamen.},
Journal = {Annals of neurology, UNITED STATES},
Volume = {22},
Number = {4},
Pages = {546-50},
Year = {1987},
Month = {October},
ISSN = {0364-5134},
Keywords = {Humans • Magnetic Resonance Imaging* • Multiple
Sclerosis • Putamen • Thalamus • diagnosis*
• pathology*},
Abstract = {High-field strength (1.5 Tesla) magnetic resonance imaging
in 15 patients with multiple and extensive white-matter
lesions and clinically definite multiple sclerosis
delineated a previously undescribed finding of abnormally
decreased signal intensity on T2-weighted images in the
thalamus and putamen. The decreased signal intensity
(preferential decreased T2 relaxation time) is most likely
to be related to abnormally increased iron accumulation
causing local magnetic field heterogeneities.},
Key = {fds132879}
}
@booklet{Drayer87,
Author = {Drayer, BP and Burger, P and Hurwitz, B and Dawson, D and Cain, J and Leong, J and Herfkens, R and Johnson, GA},
Title = {Magnetic resonance imaging in multiple sclerosis: decreased
signal in thalamus and putamen.},
Journal = {Ann Neurol},
Volume = {22},
Number = {4},
Pages = {546-550},
Year = {1987},
Month = {October},
ISSN = {0364-5134},
url = {http://www.ncbi.nlm.nih.gov/pubmed/3435073},
Abstract = {High-field strength (1.5 Tesla) magnetic resonance imaging
in 15 patients with multiple and extensive white-matter
lesions and clinically definite multiple sclerosis
delineated a previously undescribed finding of abnormally
decreased signal intensity on T2-weighted images in the
thalamus and putamen. The decreased signal intensity
(preferential decreased T2 relaxation time) is most likely
to be related to abnormally increased iron accumulation
causing local magnetic field heterogeneities.},
Doi = {10.1002/ana.410220418},
Key = {Drayer87}
}
@article{fds132827,
Author = {JC Hsu and GA Johnson and WM Smith and KA Reimer and RE
Ideker},
Title = {Magnetic resonance imaging of chronic myocardial infarcts in
formalin-fixed human autopsy hearts.},
Journal = {Circulation, UNITED STATES},
Volume = {89},
Number = {5},
Pages = {2133-40},
Year = {1994},
Month = {May},
ISSN = {0009-7322},
Keywords = {Algorithms • Formaldehyde • Fourier Analysis
• Humans • Image Processing, Computer-Assisted
• Magnetic Resonance Imaging • Magnetic Resonance
Spectroscopy • Myocardial Infarction • Myocardium
• Signal Processing, Computer-Assisted • Tissue
Fixation • methods • methods* •
pathology*},
Abstract = {BACKGROUND: In post-myocardial infarction patients,
three-dimensional structure of the infarct as well as
infarct size are likely to be important factors affecting
mortality, cardiac function, and arrhythmias. Current
morphological methods for determining three-dimensional
infarct structure in autopsied hearts are inexact and time
consuming. The cardiac magnetic resonance imaging techniques
used in living patients have shown potential in determining
infarct size and structure but have limited resolution for
morphometric postmortem studies. The recent development of
magnetic resonance microscopy raises the possibility that
three-dimensional infarct structure can be quantified at
microscopic levels in autopsied hearts. The purpose of this
study was to determine the ability of magnetic resonance
imaging at different spatial resolutions to differentiate
infarcted from noninfarcted myocardium. METHODS AND RESULTS:
Magnetic resonance imaging was performed at 2.0 T on cross
sections taken from 10 autopsied hearts containing old
myocardial infarcts. T1 was derived from six images with
repetition times (TRs) for each image ranging from 100 to
3200 milliseconds. T2 was derived from multi-echo images
with echo times (TEs) ranging from 10 to 60 milliseconds.
Resolution was approximately 400 x 400 microns in 2-mm-thick
slices. Sites of infarcted and noninfarcted tissue were
identified from histological sections taken from each slice,
and the T1 and T2 values of these sites were obtained.
Microscopic images were acquired with voxels of 100 x 100 x
625 microns, representing tissue volumes more than 1000-fold
smaller than conventional clinical images. In all cases, T1
of infarcted tissue (459 +/- 266 milliseconds, mean +/- SD)
was greater than that of noninfarcted tissue (272 +/- 163
milliseconds). Also, in all cases, T2 of infarcted tissue
(49 +/- 14 milliseconds) was greater than that of
noninfarcted tissue (35 +/- 8 milliseconds). CONCLUSIONS: T1
and T2 values for infarcted tissue are significantly
different from those of noninfarcted tissue (P < .001).
Based on these findings, it should be possible to develop
techniques to perform three-dimensional imaging and
quantitation of infarcts with a resolution of 400 microns or
less. When volumetric three-dimensional imaging was
performed using a T1-weighted sequence, the resulting 256(3)
arrays supported isotropic resolution at 400 microns (voxel
volume, 0.064 mm3). Subsequent volume rendering using a
compositing algorithm clearly shows the infarcted areas in
three dimensions. The techniques demonstrate the potential
for quantitative three-dimensional cardiac morphometry using
magnetic resonance imaging.},
Key = {fds132827}
}
@booklet{Hsu94,
Author = {Hsu, JC and Johnson, GA and Smith, WM and Reimer, KA and Ideker,
RE},
Title = {Magnetic resonance imaging of chronic myocardial infarcts in
formalin-fixed human autopsy hearts.},
Journal = {Circulation},
Volume = {89},
Number = {5},
Pages = {2133-2140},
Year = {1994},
Month = {May},
ISSN = {0009-7322},
url = {http://www.ncbi.nlm.nih.gov/pubmed/8181138},
Abstract = {BACKGROUND: In post-myocardial infarction patients,
three-dimensional structure of the infarct as well as
infarct size are likely to be important factors affecting
mortality, cardiac function, and arrhythmias. Current
morphological methods for determining three-dimensional
infarct structure in autopsied hearts are inexact and time
consuming. The cardiac magnetic resonance imaging techniques
used in living patients have shown potential in determining
infarct size and structure but have limited resolution for
morphometric postmortem studies. The recent development of
magnetic resonance microscopy raises the possibility that
three-dimensional infarct structure can be quantified at
microscopic levels in autopsied hearts. The purpose of this
study was to determine the ability of magnetic resonance
imaging at different spatial resolutions to differentiate
infarcted from noninfarcted myocardium. METHODS AND RESULTS:
Magnetic resonance imaging was performed at 2.0 T on cross
sections taken from 10 autopsied hearts containing old
myocardial infarcts. T1 was derived from six images with
repetition times (TRs) for each image ranging from 100 to
3200 milliseconds. T2 was derived from multi-echo images
with echo times (TEs) ranging from 10 to 60 milliseconds.
Resolution was approximately 400 x 400 microns in 2-mm-thick
slices. Sites of infarcted and noninfarcted tissue were
identified from histological sections taken from each slice,
and the T1 and T2 values of these sites were obtained.
Microscopic images were acquired with voxels of 100 x 100 x
625 microns, representing tissue volumes more than 1000-fold
smaller than conventional clinical images. In all cases, T1
of infarcted tissue (459 +/- 266 milliseconds, mean +/- SD)
was greater than that of noninfarcted tissue (272 +/- 163
milliseconds). Also, in all cases, T2 of infarcted tissue
(49 +/- 14 milliseconds) was greater than that of
noninfarcted tissue (35 +/- 8 milliseconds). CONCLUSIONS: T1
and T2 values for infarcted tissue are significantly
different from those of noninfarcted tissue (P < .001).
Based on these findings, it should be possible to develop
techniques to perform three-dimensional imaging and
quantitation of infarcts with a resolution of 400 microns or
less. When volumetric three-dimensional imaging was
performed using a T1-weighted sequence, the resulting 256(3)
arrays supported isotropic resolution at 400 microns (voxel
volume, 0.064 mm3). Subsequent volume rendering using a
compositing algorithm clearly shows the infarcted areas in
three dimensions. The techniques demonstrate the potential
for quantitative three-dimensional cardiac morphometry using
magnetic resonance imaging.},
Doi = {10.1161/01.cir.89.5.2133},
Key = {Hsu94}
}
@article{fds268762,
Author = {Bcnveniste, H and Hüttemeier, PC and Qiu, H and Steele, S and Hedlund,
LW and Allan Johnson and G},
Title = {Magnetic resonance imaging of continuous spinal anesthesia
with hyperbaric lidocaine: Root or white matter
lesion?},
Journal = {Regional Anesthesia},
Volume = {22},
Number = {2 SUPPL.},
Pages = {15},
Year = {1997},
Month = {December},
ISSN = {0146-521X},
Abstract = {Introduction: Persistent neurologic deficits in patients
after continuous spinal anesthesia with hyperbaric lidocaine
may have been erroneously characterized as a cauda equina
syndrome (1,2). First, clinical reports of symptoms rarely
include pain (typical for cauda equina lesions) but most
often consist of saddle anesthesia, paraplegia and sphincter
dysfunction (typical for conus medullaris lesions).
Secondly, spinal cord damage in dogs after intrathecal
procaine (3) or lidocaine (4) is confined only to the myelin
sheath beneath the pia mater and not nerve roots. In this
study we use diffusion-weighted magnetic resonance imaging
(DWI) to further characterize hyperbaric lidocaine-induced
spinal cord damage. DWI measures diffusion of free protons
and is a very sensitive detector of white or gray matter
injury (5). Normal spinal cord gray and white matter as well
as nerve roots are clearly visualized by DWI because the
proton diffusion coefficient (DC) - the critical parameter
measured with DWI - within these tissues differs. The effect
of hyperbaric 5% lidocaine on the spinal cord diffusion
coefficient will be compared with those found in ischémie
conditions. Methods: The study was approved by the Duke
University Animal Care Institutional Committee. Nine female
adult Fisher rats (weighing 150-190g) were anesthetized with
isoflurane, intubated and mechanically ventilated. Catheters
were inserted into the right external jugular vein and
carotid artery. Heart rate, arterial blood pressure and body
temperature was monitored continuously. Diffusion-weighted
MR images (DWI) were acquired at the level of C6 in five
rats before and after ischemia induced by cardiac arrest. In
another four rats catheters were first placed intrathecally
via the atlanto-occipital membrane. Subsequently, DWI's were
acquired at the level of C6 before and during 3 hr
continuous intrathecal perfusion with 5% hyperbaric
lidocaine. Hemodynamic stability during lidocaine
administration was maintained with i.v. hydration, atropine
and ephedrine as needed. All imaging was done on a 7 T
magnet using a standard spin echo diffusion pulse sequence
(5). Results: Ischemia reduced the diffusion coefficient
(DC) of white matter by 25%, gray matter by 45% and nerve
roots/dorsal root ganglia by 35%. Figure 1 shows that one
hour of continuous intrathecal administration of 5%
hyperbaric lidocaine reduces the DC of white matter by 15%
and that the changes are not as profound as those found
during ischemia (results are presented as mean ± SD).
Figure 2 shows that in contrast to white matter, DCs of
adjacent nerve roots are unaffected by 5 % hyperbaric
lidocaine. Discussion: The present study has led to the
following conclusion: (1) diffusion-weighted imaging of
spinal cord in rats with indwelling intrathecal catheters is
possible in vivo, (2) the diffusion coefficient of white
matter is reduced by 15% after 1 hr of 5% hyperbaric
lidocaine indicating onset of cytotoxic edema, (3) the
lidocaine-induced changes in the white matter DC do not
reach ischémie levels, (4) adjacent gray matter as well as
nerve roots are unaffected. Ongoing work will provide
information as to the exact pathophysiological sequence of
events which lead to irreversible spinal cord damage during
continuous spinal anesthesia with high dose hyperbaric 5%
lidocaine. It is our goal that this research will be
applicable to local anesthesia toxicity studies and useful
as a future neurotoxicity screening tool for new intralhecal
drugs designed for both anesthesia and analgesia.},
Key = {fds268762}
}
@booklet{Smith99,
Author = {Smith, BR and Huff, DS and Johnson, GA},
Title = {Magnetic resonance imaging of embryos: an Internet resource
for the study of embryonic development.},
Journal = {Comput Med Imaging Graph},
Volume = {23},
Number = {1},
Pages = {33-40},
Year = {1999},
ISSN = {0895-6111},
url = {http://www.ncbi.nlm.nih.gov/pubmed/10091866},
Abstract = {The recent amassing of gene expression data to study
development in mammals has led to an increased demand for
access to human embryological data. The difficulty of
obtaining well-preserved human embryos presents an important
challenge to studying human development. The
Multidimensional Human Embryo project is generating an image
data set based on magnetic resonance microscopy of specimens
from the highly respected Carnegie Collection of Human
Embryos. The data are available from a web site to
facilitate the work of clinicians, investigators, and
students of human development. A consequence of the project
will be to preserve a highly respected, yet impermanent,
collection of human embryos and minimize the need for
collecting new specimens.},
Doi = {10.1016/s0895-6111(98)00061-5},
Key = {Smith99}
}
@article{fds132767,
Author = {BR Smith and DS Huff and GA Johnson},
Title = {Magnetic resonance imaging of embryos: an Internet resource
for the study of embryonic development.},
Journal = {Computerized medical imaging and graphics : the official
journal of the Computerized Medical Imaging Society, UNITED
STATES},
Volume = {23},
Number = {1},
Pages = {33-40},
ISSN = {0895-6111},
Keywords = {Embryo • Humans • Image Processing,
Computer-Assisted* • Internet* • Magnetic
Resonance Imaging* • anatomy & histology*},
Abstract = {The recent amassing of gene expression data to study
development in mammals has led to an increased demand for
access to human embryological data. The difficulty of
obtaining well-preserved human embryos presents an important
challenge to studying human development. The
Multidimensional Human Embryo project is generating an image
data set based on magnetic resonance microscopy of specimens
from the highly respected Carnegie Collection of Human
Embryos. The data are available from a web site to
facilitate the work of clinicians, investigators, and
students of human development. A consequence of the project
will be to preserve a highly respected, yet impermanent,
collection of human embryos and minimize the need for
collecting new specimens.},
Key = {fds132767}
}
@article{fds268702,
Author = {Cutlip, RG and Hollander, MS and Johnson, GA and Johnson, BW and Friend,
SA and Baker, BA},
Title = {Magnetic resonance imaging of graded skeletal muscle injury
in live rats.},
Journal = {Environ Health Insights},
Volume = {8},
Number = {Suppl 1},
Pages = {31-39},
Year = {2014},
url = {http://hdl.handle.net/10161/10309 Duke open
access},
Abstract = {INTRODUCTION: Increasing number of stretch-shortening
contractions (SSCs) results in increased muscle injury.
METHODS: Fischer Hybrid rats were acutely exposed to an
increasing number of SSCs in vivo using a custom-designed
dynamometer. Magnetic resonance imaging (MRI) imaging was
conducted 72 hours after exposure when rats were infused
with Prohance and imaged using a 7T rodent MRI system (GE
Epic 12.0). Images were acquired in the transverse plane
with typically 60 total slices acquired covering the entire
length of the hind legs. Rats were euthanized after MRI, the
lower limbs removed, and tibialis anterior muscles were
prepared for histology and quantified stereology. RESULTS:
Stereological analyses showed myofiber degeneration, and
cellular infiltrates significantly increased following 70
and 150 SSC exposure compared to controls. MRI images
revealed that the percent affected area significantly
increased with exposure in all SSC groups in a graded
fashion. Signal intensity also significantly increased with
increasing SSC repetitions. DISCUSSION: These results
suggest that contrast-enhanced MRI has the sensitivity to
differentiate specific degrees of skeletal muscle strain
injury, and imaging data are specifically representative of
cellular histopathology quantified via stereological
analyses.},
Doi = {10.4137/EHI.S15255},
Key = {fds268702}
}
@article{fds132815,
Author = {GA Johnson and MB Thompson and GP Cofer and D Campen and RR
Maronpot},
Title = {Magnetic resonance imaging of hepatic neoplasms in the
rat.},
Journal = {Veterinary pathology, UNITED STATES},
Volume = {26},
Number = {4},
Pages = {303-8},
Year = {1989},
Month = {July},
ISSN = {0300-9858},
Keywords = {Animals • Female • Liver Neoplasms, Experimental
• Magnetic Resonance Imaging* • Ovariectomy •
Rats • Rats, Inbred Strains • diagnosis •
pathology* • veterinary},
Abstract = {Magnetic resonance imaging (MRI) at microscopic resolution
was done on a live rat that had chemically induced hepatic
neoplasms. Beginning at the anterior aspect of the liver, 16
contiguous transaxial slices (each 1.25 mm thick) were
produced using three-dimensional Fourier transform
sequences. The rat had been treated with diethylnitrosamine
(200 mg/kg) at 70 days of age, and, subsequently, received
periodic implants of 17a-ethynylestradiol for 60 weeks.
Carr-Purcell-Meiboom-Gill (CPMG) sequences (repetition time
= 2,000 and echo time = 20, 40, 60, 80 ms) were done to give
quantitative measures of spin-spin relaxation times (T2).
Pixel-by-pixel curve fitting from these multiple images
yielded calculated T2 images. Histologic evaluation of three
abnormal areas in the liver revealed solid and cystic
hepatocellular adenomas. Although lesions were evident in
early-echo images of the CPMG sequence, they were more
apparent in the late-echo images. This was consistent with
longer T2 relaxation times for the lesions. The voxels of
dimensions (230 x 230 x 1,250 microns) permitted resolution
of volume elements less than 0.07 mm3. This in turn
permitted clear delineation of focal lesions less than 3 mm
in diameter. The potential for MRI at microscopic resolution
in toxicologic research is clearly demonstrated.},
Key = {fds132815}
}
@booklet{Johnson89a,
Author = {Johnson, GA and Thompson, MB and Cofer, GP and Campen, D and Maronpot,
RR},
Title = {Magnetic resonance imaging of hepatic neoplasms in the
rat.},
Journal = {Vet Pathol},
Volume = {26},
Number = {4},
Pages = {303-308},
Year = {1989},
Month = {July},
ISSN = {0300-9858},
url = {http://www.ncbi.nlm.nih.gov/pubmed/2763419},
Abstract = {Magnetic resonance imaging (MRI) at microscopic resolution
was done on a live rat that had chemically induced hepatic
neoplasms. Beginning at the anterior aspect of the liver, 16
contiguous transaxial slices (each 1.25 mm thick) were
produced using three-dimensional Fourier transform
sequences. The rat had been treated with diethylnitrosamine
(200 mg/kg) at 70 days of age, and, subsequently, received
periodic implants of 17a-ethynylestradiol for 60 weeks.
Carr-Purcell-Meiboom-Gill (CPMG) sequences (repetition time
= 2,000 and echo time = 20, 40, 60, 80 ms) were done to give
quantitative measures of spin-spin relaxation times (T2).
Pixel-by-pixel curve fitting from these multiple images
yielded calculated T2 images. Histologic evaluation of three
abnormal areas in the liver revealed solid and cystic
hepatocellular adenomas. Although lesions were evident in
early-echo images of the CPMG sequence, they were more
apparent in the late-echo images. This was consistent with
longer T2 relaxation times for the lesions. The voxels of
dimensions (230 x 230 x 1,250 microns) permitted resolution
of volume elements less than 0.07 mm3. This in turn
permitted clear delineation of focal lesions less than 3 mm
in diameter. The potential for MRI at microscopic resolution
in toxicologic research is clearly demonstrated.},
Doi = {10.1177/030098588902600403},
Key = {Johnson89a}
}
@booklet{Levron98,
Author = {Levron, D and Walter, DK and Appelt, S and Fitzgerald, RJ and Kahn, D and Korbly, SE and Sauer, KL and Happer, W and Earles, TL and Mawst, LJ and al,
E},
Title = {Magnetic resonance imaging of hyperpolarized 129Xe produced
by spin exchange with diode-laser pumped
Cs},
Journal = {Applied Physics Letters},
Volume = {73},
Number = {18},
Pages = {2666-2668},
Publisher = {AIP Publishing},
Year = {1998},
url = {http://dx.doi.org/10.1063/1.122547},
Abstract = {We report the results of experiments leading to the
production of an image of a polarized 129Xe sample prepared
by spin exchange with Cs, optically pumped with a spectrally
narrowed 894.3 nm diode laser. Representative images of the
average electron spin polarization are shown. Appreciable
cesium electron polarization values were achieved, and a
nuclear polarization of about 2.5% was measured for 129Xe.
The absolute nuclear polarization was measured by
water-calibrated free induction decay of the nuclear
magnetic resonance signal, and the polarized xenon imaged
using a 2 T magnetic resonance imaging system. © 1998
American Institute of Physics.},
Doi = {10.1063/1.122547},
Key = {Levron98}
}
@article{fds268776,
Author = {Shattuck, M and Behringer, R and Geordiadis, J and Johnson,
GA},
Title = {Magnetic resonance imaging of interstitial velocity
distributions in porous media},
Journal = {American Society of Mechanical Engineers, Fluids Engineering
Division (Publication) FED},
Volume = {125},
Pages = {39-45},
Year = {1991},
Month = {December},
Abstract = {In this article we report on a promising application of
Magnetic Resonance Imaging (MRI), which can measure local
interstitial velocity distributions inside fully-saturated
porous media. We have extended the standard
three-dimensional MRI sequence to include local velocity
information. During flow, the moving spins of the fluid (in
this case water) accumulate phase in the presence of
magnetic field gradients. This phase is proportional to the
local velocity. By repeating the measurement under the
influence of different magnetic gradient strengths, we can
reconstruct the velocity distribution in each volume element
(voxel). Using this technique to study pressure-driven flow
through a fully-saturated, cylindrical packed bed, we have
observed flow channeling near the walls and an exponential
distribution of velocities.},
Key = {fds268776}
}
@article{fds343279,
Author = {Shattuck, M and Behringer, R and Geordiadis, J and Johnson,
GA},
Title = {Magnetic resonance imaging of interstitial velocity
distributions in porous media},
Year = {1991},
Month = {January},
Abstract = {In this article we report on a promising application of
Magnetic Resonance Imaging (MRI), which can measure local
interstitial velocity distributions inside fully-saturated
porous media. We have extended the standard
three-dimensional MRI sequence to include local velocity
information. During flow, the moving spins of the fluid (in
this case water) accumulate phase in the presence of
magnetic field gradients. This phase is proportional to the
local velocity. By repeating the measurement under the
influence of different magnetic gradient strengths, we can
reconstruct the velocity distribution in each volume element
(voxel). Using this technique to study pressure-driven flow
through a fully-saturated, cylindrical packed bed, we have
observed flow channeling near the walls and an exponential
distribution of velocities. (A)},
Key = {fds343279}
}
@article{fds340705,
Author = {Shattuck, M and Behringer, R and Geordiadis, J and Johnson,
GA},
Title = {Magnetic resonance imaging of interstitial velocity
distributions in porous media},
Year = {1991},
Month = {January},
Abstract = {In this article we report on a promising application of
Magnetic Resonance Imaging (MRI), which can measure local
interstitial velocity distributions inside fully-saturated
porous media. We have extended the standard
three-dimensional MRI sequence to include local velocity
information. During flow, the moving spins of the fluid (in
this case water) accumulate phase in the presence of
magnetic field gradients. This phase is proportional to the
local velocity. By repeating the measurement under the
influence of different magnetic gradient strengths, we can
reconstruct the velocity distribution in each volume element
(voxel). Using this technique to study pressure-driven flow
through a fully-saturated, cylindrical packed bed, we have
observed flow channeling near the walls and an exponential
distribution of velocities. (A)},
Key = {fds340705}
}
@booklet{Veres93,
Author = {VERES, JS and COFER, GP and JOHNSON, GA},
Title = {Magnetic resonance imaging of leaves},
Journal = {New Phytologist},
Volume = {123},
Number = {4},
Pages = {769-774},
Publisher = {WILEY},
Year = {1993},
Month = {January},
ISSN = {0028-646X},
url = {http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:A1993LE53500015&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=47d3190e77e5a3a53558812f597b0b92},
Abstract = {Several aspects of leaf anatomy and morphology make it
difficult to image leaves with magnetic resonance imaging.
Since most fully expanded leaves contain much intercellular
air space and are often thin in one plane, relatively few
water protons are available to provide signal. Their
heterogeneous structure and the presence of many air‐water
interfaces cause imaging problems as a result of differences
in magnetic susceptibility of tissues and cells. Significant
progress has been made in overcoming these difficulties,
primarily by greatly reducing the echo time, permitting in
vivo imaging of leaves at 7 and 9.4 Tesla with an in‐plane
resolution of less than 15 μm2. Examples are shown and the
potential for gaining information regarding in vivo water
relations of leaves is discussed. Copyright © 1993, Wiley
Blackwell. All rights reserved},
Doi = {10.1111/j.1469-8137.1993.tb03788.x},
Key = {Veres93}
}
@booklet{Stern90,
Author = {Stern, RL and Johnson, GA and Ravin, CE},
Title = {Magnetic resonance imaging of the thoracic cavity using a
paused 3DFT acquisition technique.},
Journal = {Magn Reson Imaging},
Volume = {8},
Number = {6},
Pages = {747-753},
Year = {1990},
ISSN = {0730-725X},
url = {http://www.ncbi.nlm.nih.gov/pubmed/2266801},
Abstract = {A new pulse sequence designed for magnetic resonance imaging
of the entire thoracic cavity is described. This sequence,
called 3DPAUSE, is a rapid three-dimensional Fourier
transform (3DFT) sequences with periodic pauses for
breathing and additional rf pulses after each pause to
restore the magnetization to steady-state before data
acquisition resumes. Cardiac motion artifacts are
effectively removed by signal averaging. Respiratory motion
artifacts are removed by breath hold. Image artifacts caused
by an inadequate number of pauses or by inappropriate
placement of the pauses within a scan are shown, and ways to
avoid these artifacts are discussed. 3DPAUSE provides the
ability to acquire three-dimensional arrays in the thoracic
cavity with minimal artifacts from respiratory and cardiac
motions in a clinically reasonable time.},
Doi = {10.1016/0730-725x(90)90010-y},
Key = {Stern90}
}
@article{fds132801,
Author = {RL Stern and GA Johnson and CE Ravin},
Title = {Magnetic resonance imaging of the thoracic cavity using a
paused 3DFT acquisition technique.},
Journal = {Magnetic resonance imaging, UNITED STATES},
Volume = {8},
Number = {6},
Pages = {747-53},
Year = {1990},
ISSN = {0730-725X},
Keywords = {Animals • Dogs • Humans • Magnetic Resonance
Imaging • Thorax • anatomy & histology* •
methods*},
Abstract = {A new pulse sequence designed for magnetic resonance imaging
of the entire thoracic cavity is described. This sequence,
called 3DPAUSE, is a rapid three-dimensional Fourier
transform (3DFT) sequences with periodic pauses for
breathing and additional rf pulses after each pause to
restore the magnetization to steady-state before data
acquisition resumes. Cardiac motion artifacts are
effectively removed by signal averaging. Respiratory motion
artifacts are removed by breath hold. Image artifacts caused
by an inadequate number of pauses or by inappropriate
placement of the pauses within a scan are shown, and ways to
avoid these artifacts are discussed. 3DPAUSE provides the
ability to acquire three-dimensional arrays in the thoracic
cavity with minimal artifacts from respiratory and cardiac
motions in a clinically reasonable time.},
Key = {fds132801}
}
@article{fds325754,
Author = {Sulik, KK and O'Leary-Moore, SK and Parnell, SE and Godin, EA and Styner, MA and Lipinski, RJ and Johnson, GA},
Title = {MAGNETIC RESONANCE IMAGING-BASED ANALYSES OF A FETAL ALCOHOL
SPECTRUM DISORDERS MOUSE MODEL},
Journal = {ALCOHOLISM-CLINICAL AND EXPERIMENTAL RESEARCH},
Volume = {34},
Number = {8},
Pages = {31A-31A},
Year = {2010},
Key = {fds325754}
}
@article{fds376637,
Author = {Zhou, X and Johnson, GA},
Title = {Magnetic resonance microscopy},
Pages = {5-42-6-14},
Booktitle = {Medical Imaging: Principles and Practices},
Year = {2012},
Month = {January},
ISBN = {9781439871027},
url = {http://dx.doi.org/10.1201/b12939},
Abstract = {Visualization of internal structures of opaque biologic
objects is essential in many biomedical studies. Limited by
the penetration depth of the probing sources (photons and
electrons) and the lack of endogenous contrast, conventional
forms of microscopy such as optical microscopy and electron
microscopy require tissues to be sectioned into thin slices
and stained with organic chemicals or heavy-metal compounds
prior to examination. These invasive and destructive
procedures, as well as the harmful radiation in the case of
electron microscopy, make it difficult to obtain
three-dimensional information and virtually impossible to
study biologic tissues in vivo.},
Doi = {10.1201/b12939},
Key = {fds376637}
}
@article{fds341990,
Author = {Zhou, X and Johnson, GA},
Title = {Magnetic resonance microscopy},
Pages = {15-1-15-14},
Booktitle = {Medical Devices and Systems},
Year = {2006},
Month = {January},
ISBN = {9780849321221},
Abstract = {Visualization of internal structures of opaque biologic
objects is essential in many biomedical studies. Limited by
the penetration depth of the probing sources (photons and
electrons) and the lack of endogenous contrast, conventional
forms of microscopy such as optical microscopy and electron
microscopy require tissues to be sectioned into thin slices
and stained with organic chemicals or heavy-metal compounds
prior to examination. These invasive and destructive
procedures, as well as the harmful radiation in the case of
electron microscopy, make it difficult to obtain
three-dimensional information and virtually impossible to
study biologic tissues in vivo.},
Key = {fds341990}
}
@article{fds342055,
Author = {Zhou, X and Allan Johnson and G},
Title = {Magnetic resonance microscopy},
Pages = {13-1-13-14},
Booktitle = {Biomedical Imaging},
Year = {2003},
Month = {January},
ISBN = {9780849318108},
Abstract = {Visualization of internal structures of opaque biologic
objects is essential in many biomedical studies. Limited by
the penetration depth of the probing sources (photons and
electrons) and the lack of endogenous contrast, conventional
forms of microscopy such as optical microscopy and electron
microscopy require tissues to be sectioned into thin slices
and stained with organic chemicals or heavy-metal compounds
prior to examination. These invasive and destructive
procedures, as well as the harmful radiation in the case of
electron microscopy, make it difficult to obtain
three-dimensional information and virtually impossible to
study biologic tissues in vivo.},
Key = {fds342055}
}
@article{fds268751,
Author = {O'Leary-Moore, SK and Parnell, SE and Godin, EA and Johnson, GA and Styner, M and Oguz, I and Budin, F and Jiang, Y and Dehart, DB and Sulik,
KK},
Title = {MAGNETIC RESONANCE MICROSCOPY AND DIFFUSION TENSOR IMAGING
DEFINE STAGE-DEPENDENT CHANGES IN BRAIN MORPHOLOGY AFTER
PRENATAL ETHANOL EXPOSURE IN MICE},
Journal = {ALCOHOLISM-CLINICAL AND EXPERIMENTAL RESEARCH},
Volume = {34},
Number = {6},
Pages = {298A-298A},
Publisher = {WILEY-BLACKWELL},
Year = {2010},
Month = {June},
ISSN = {0145-6008},
url = {http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000291641500215&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=47d3190e77e5a3a53558812f597b0b92},
Key = {fds268751}
}
@booklet{Delnomdedieu98,
Author = {M. Delnomdedieu and L. W. Hedlund and R. R. Maronpot and G.
A. Johnson},
Title = {Magnetic resonance microscopy and histopathology:
Comparative approach of bromobenzene-induced hepatotoxicity
in the rat},
Journal = {Hepatology},
Volume = {27},
Number = {2},
Pages = {526 -- 532},
Year = {1998},
Month = {February},
Key = {Delnomdedieu98}
}
@article{fds269045,
Author = {Delnomdedieu, M and Hedlund, LW and Maronpot, RR and Johnson,
GA},
Title = {Magnetic resonance microscopy and histopathology:
comparative approach of bromobenzene-induced hepatotoxicity
in the rat.},
Journal = {Hepatology},
Volume = {27},
Number = {2},
Pages = {526-532},
Year = {1998},
Month = {February},
ISSN = {0270-9139},
url = {http://www.ncbi.nlm.nih.gov/pubmed/9462653},
Keywords = {Animals • Bromobenzenes* • Female •
Histocytochemistry • Liver Diseases • Microscopy
• Rats • Rats, Inbred F344 • Reproducibility
of Results • Sensitivity and Specificity •
chemically induced • methods* •
pathology*},
Abstract = {The development of magnetic resonance (MR) microscopy has
provided new approaches to histology and histopathology.
Recent work has shown the promise of increased sensitivity
in animal models of chemically induced hepatotoxicity.
However, the field is so new that there is little experience
to relate changes seen in MR micrographs to the more
traditional optical images stained with hematoxylin and
eosin. This work compares the sensitivity and
reproducibility of MR microscopy with conventional
histopathology in detecting bromobenzene-induced
hepatotoxicity in the rat. A time-course study was
undertaken to provide a range of histopathologies. Specimens
were studied at 24, 48, 72, and 96 hours after exposure to
10% of the median lethal dose of bromobenzene. Using 4
animals per group (a total of 32 rats) added statistical
significance to the study and defined a range of interanimal
variability over 96 hours. This work shows that MR
microscopy, besides being nondestructive and
three-dimensional, is at least as sensitive as conventional
hematoxylin-eosin staining in detecting bromobenzene-induced
centrilobular lesions and recovery of the hepatocellular
architecture in the rat. This study further suggests that,
as we begin to understand the underlying mechanisms of
contrast in MR histology, MR may, in fact, supply even
higher specificity than more traditional studies: variations
were observed in MR images of treated livers at a given time
point that could be not be differentiated based on the
grading of necrosis and inflammation on hematoxylin-eosin-stained
sections.},
Doi = {10.1002/hep.510270229},
Key = {fds269045}
}
@article{fds268768,
Author = {Godin, EA and O'Leary-Moore, SK and Khan, AA and Parnell, SE and Ament,
JJ and Dehart, DB and Johnson, BW and Allan Johnson and G and Styner, MA and Sulik, KK},
Title = {Magnetic resonance microscopy defines ethanol-induced brain
abnormalities in prenatal mice: effects of acute insult on
gestational day 7.},
Journal = {Alcohol Clin Exp Res},
Volume = {34},
Number = {1},
Pages = {98-111},
Year = {2010},
Month = {January},
ISSN = {0145-6008},
url = {http://dx.doi.org/10.1111/j.1530-0277.2009.01071.x},
Abstract = {BACKGROUND: This magnetic resonance microscopy (MRM)-based
report is the second in a series designed to illustrate the
spectrum of craniofacial and central nervous system (CNS)
dysmorphia resulting from single- and multiple-day maternal
ethanol treatment. The study described in this report
examined the consequences of ethanol exposure on gestational
day (GD) 7 in mice, a time in development when gastrulation
and neural plate development begins; corresponding to the
mid- to late third week postfertilization in humans. Acute
GD 7 ethanol exposure in mice has previously been shown to
result in CNS defects consistent with holoprosencephaly
(HPE) and craniofacial anomalies typical of those in Fetal
Alcohol Syndrome (FAS). MRM has facilitated further
definition of the range of GD 7 ethanol-induced defects.
METHODS: C57Bl/6J female mice were intraperitoneally (i.p.)
administered vehicle or 2 injections of 2.9 g/kg ethanol on
day 7 of pregnancy. Stage-matched control and
ethanol-exposed GD 17 fetuses selected for imaging were
immersion fixed in a Bouins/Prohance solution. MRM was
conducted at either 7.0 Tesla (T) or 9.4 T. Resulting 29
microm isotropic spatial resolution scans were segmented and
reconstructed to provide 3D images. Linear and volumetric
brain measures, as well as morphological features, were
compared for control and ethanol-exposed fetuses. Following
MRM, selected specimens were processed for routine histology
and light microscopic examination. RESULTS: Gestational day
7 ethanol exposure resulted in a spectrum of median facial
and forebrain deficiencies, as expected. This range of
abnormalities falls within the HPE spectrum; a spectrum for
which facial dysmorphology is consistent with and typically
is predictive of that of the forebrain. In addition, other
defects including median facial cleft, cleft palate,
micrognathia, pituitary agenesis, and third ventricular
dilatation were identified. MRM analyses also revealed
cerebral cortical dysplasia/heterotopias resulting from this
acute, early insult and facilitated a subsequent focused
histological investigation of these defects. CONCLUSIONS:
Individual MRM scans and 3D reconstructions of fetal mouse
brains have facilitated demonstration of a broad range of GD
7 ethanol-induced morphological abnormality. These results,
including the discovery of cerebral cortical heterotopias,
elucidate the teratogenic potential of ethanol insult during
the third week of human prenatal development.},
Doi = {10.1111/j.1530-0277.2009.01071.x},
Key = {fds268768}
}
@article{fds268767,
Author = {Parnell, SE and O'Leary-Moore, SK and Godin, EA and Dehart, DB and Johnson, BW and Allan Johnson and G and Styner, MA and Sulik,
KK},
Title = {Magnetic resonance microscopy defines ethanol-induced brain
abnormalities in prenatal mice: effects of acute insult on
gestational day 8.},
Journal = {Alcohol Clin Exp Res},
Volume = {33},
Number = {6},
Pages = {1001-1011},
Year = {2009},
Month = {June},
ISSN = {0145-6008},
url = {http://dx.doi.org/10.1111/j.1530-0277.2009.00921.x},
Abstract = {BACKGROUND: Magnetic resonance microscopy (MRM), magnetic
resonance imaging (MRI) at microscopic levels, provides
unprecedented opportunities to aid in defining the full
spectrum of ethanol's insult to the developing brain. This
is the first in a series of reports that, collectively, will
provide an MRM-based atlas of developmental stage-dependent
structural brain abnormalities in a Fetal Alcohol Spectrum
Disorders (FASD) mouse model. The ethanol exposure time and
developmental stage examined for this report is gestational
day (GD) 8 in mice, when the embryos are at early
neurulation stages; stages present in humans early in the
fourth week postfertilization. METHODS: For this study,
pregnant C57Bl/6J mice were administered an ethanol dosage
of 2.8 g/kg intraperitoneally at 8 days, 0 hour and again at
8 days, 4 hours postfertilization. On GD 17, fetuses that
were selected for MRM analyses were immersion fixed in a
Bouin's/Prohance solution. Control fetuses from
vehicle-treated dams were stage-matched to those that were
ethanol-exposed. The fetal mice were scanned ex vivo at 7.0
T and 512 x 512 x 1024 image arrays were acquired using 3-D
spin warp encoding. The resulting 29 microm (isotropic)
resolution images were processed using ITK-SNAP, a 3-D
segmentation/visualization tool. Linear and volume
measurements were determined for selected brain, head, and
body regions of each specimen. Comparisons were made between
control and treated fetuses, with an emphasis on determining
(dis)proportionate changes in specific brain regions.
RESULTS: As compared with controls, the crown-rump lengths
of stage-matched ethanol-exposed GD 17 fetuses were
significantly reduced, as were brain and whole body volumes.
Volume reductions were notable in every brain region
examined, with the exception of the pituitary and septal
region, and were accompanied by increased ventricular
volumes. Disproportionate regional brain volume reductions
were most marked on the right side and were significant for
the olfactory bulb, hippocampus, and cerebellum; the latter
being the most severely affected. Additionally, the septal
region and the pituitary were disproportionately large.
Linear measures were consistent with those of volume. Other
dysmorphologic features noted in the MR scans were choanal
stenosis and optic nerve coloboma. CONCLUSIONS: This study
demonstrates that exposure to ethanol occurring in mice at
stages corresponding to the human fourth week
postfertilization results in structural brain abnormalities
that are readily identifiable at fetal stages of
development. In addition to illustrating the utility of MR
microscopy for analysis of an FASD mouse model, this work
provides new information that confirms and extends human
clinical observations. It also provides a framework for
comparison of structural brain abnormalities resulting from
ethanol exposure at other developmental stages and
dosages.},
Doi = {10.1111/j.1530-0277.2009.00921.x},
Key = {fds268767}
}
@booklet{Johnson97a,
Author = {Johnson, GA and Benveniste, H and Engelhardt, RT and Qiu, H and Hedlund,
LW},
Title = {Magnetic resonance microscopy in basic studies of brain
structure and function.},
Journal = {Ann N Y Acad Sci},
Volume = {820},
Series = {ANNALS OF THE NEW YORK ACADEMY OF SCIENCES},
Pages = {139-147},
Year = {1997},
Month = {May},
ISSN = {0077-8923},
url = {http://www.ncbi.nlm.nih.gov/pubmed/9237453},
Doi = {10.1111/j.1749-6632.1997.tb46193.x},
Key = {Johnson97a}
}
@article{fds132813,
Author = {GA Johnson and H Benveniste and RT Engelhardt and H Qiu and LW
Hedlund},
Title = {Magnetic resonance microscopy in basic studies of brain
structure and function.},
Journal = {Annals of the New York Academy of Sciences, UNITED
STATES},
Volume = {820},
Pages = {139-47; discussion 147-8},
Year = {1997},
Month = {May},
ISSN = {0077-8923},
Keywords = {Animals • Brain • Diagnostic Imaging • Humans
• Microscopy • methods* • pathology •
physiology • radiography*},
Key = {fds132813}
}
@article{fds268884,
Author = {Johnson, GA and Thompson, MB and Drayer, BP and Bone,
SN},
Title = {Magnetic resonance microscopy in neurologic
models.},
Journal = {Acta Radiol Suppl},
Volume = {369},
Pages = {267-268},
Year = {1986},
ISSN = {0365-5954},
url = {http://www.ncbi.nlm.nih.gov/pubmed/2980471},
Keywords = {Animals • Brain • Chick Embryo • Magnetic
Resonance Imaging • Rats • anatomy & histology*
• cytology • methods*},
Abstract = {Magnetic resonance imaging techniques have been developed to
permit imaging with slice thickness less than 1 mm and
pixels of 50 x 50 microns. Special purpose gradient and
radiofrequency coils and three-dimensional imaging
techniques enable acquisition of images with sufficient
signal to noise to utilize these microscopic picture
elements. Live 200 g rats were imaged enabling clear
definition of gray and white matter structures. Examples
include the Sylvian aqueduct and the substantia nigra.
Three-dimensional microscopic images of live chick embryos
enabled definition of ventricles and brain parenchyma as
well as measurement of T1 over the set of 16 contiguous 1.2
mm slices.},
Key = {fds268884}
}
@article{fds268764,
Author = {Benveniste, H and Einstein, G and Kim, KR and Allan Johnson,
G},
Title = {Magnetic resonance microscopy of Alzheimer's disease: Senile
plaques - A whiter shade of pale?},
Journal = {NeuroImage},
Volume = {7},
Number = {4 PART II},
Pages = {S519},
Year = {1998},
Month = {January},
url = {http://dx.doi.org/10.1016/s1053-8119(18)31352-1},
Doi = {10.1016/s1053-8119(18)31352-1},
Key = {fds268764}
}
@article{fds132845,
Author = {GA Johnson and J Brown and PJ Kramer},
Title = {Magnetic resonance microscopy of changes in water content in
stems of transpiring plants.},
Journal = {Proceedings of the National Academy of Sciences of the
United States of America, UNITED STATES},
Volume = {84},
Number = {9},
Pages = {2752-5},
Year = {1987},
Month = {May},
ISSN = {0027-8424},
Keywords = {Magnetic Resonance Spectroscopy • Plants • Water
• metabolism*},
Abstract = {Differences in water content and degree of binding in the
various stem tissues of Pelargonium hortorum were observed
by magnetic resonance imaging. 1H images were obtained with
a resolution of 100 microns in the transverse plane and a
slice thickness of 1250 microns. It was possible to
distinguish the principal tissues of the stem by differences
in their proton density or apparent water content and spin
lattice relaxation time (T1) or degree of water binding.
Measurements were made while the plant was slowly and
actively transpiring. In the slowly transpiring plant, T1 of
various tissues ranged from an average of 659 to 865 ms with
a proton density variation of from 72 to 100%. In the
actively transpiring plant, T1 ranged from an average of 511
to 736 ms, and the proton density was reduced, ranging
between 62 and 88% of the peak value found in the slowly
transpiring plant. The fibrous sheath surrounding the
vascular tissue and the epidermal region was found to have
the highest spin density and T1. Both tissues are comprised
of relatively small thick-walled cells. Cortical and pith
parenchyma are composed of larger, thinner-walled cells with
numerous intercellular spaces and lower spin density and T1.
The differences are attributed to the higher water content
by volume in the tissue composed of smaller, more compactly
arranged cells. The resolution obtained in this work enables
clear definition of tissues in the living plant and
quantitative information concerning differences in the
distribution and extent of binding of water.},
Key = {fds132845}
}
@booklet{Johnson87d,
Author = {Johnson, GA and Brown, J and Kramer, PJ},
Title = {Magnetic resonance microscopy of changes in water content in
stems of transpiring plants.},
Journal = {Proc Natl Acad Sci U S A},
Volume = {84},
Number = {9},
Pages = {2752-2755},
Year = {1987},
Month = {May},
ISSN = {0027-8424},
url = {http://www.ncbi.nlm.nih.gov/pubmed/3472235},
Abstract = {Differences in water content and degree of binding in the
various stem tissues of Pelargonium hortorum were observed
by magnetic resonance imaging. 1H images were obtained with
a resolution of 100 microns in the transverse plane and a
slice thickness of 1250 microns. It was possible to
distinguish the principal tissues of the stem by differences
in their proton density or apparent water content and spin
lattice relaxation time (T1) or degree of water binding.
Measurements were made while the plant was slowly and
actively transpiring. In the slowly transpiring plant, T1 of
various tissues ranged from an average of 659 to 865 ms with
a proton density variation of from 72 to 100%. In the
actively transpiring plant, T1 ranged from an average of 511
to 736 ms, and the proton density was reduced, ranging
between 62 and 88% of the peak value found in the slowly
transpiring plant. The fibrous sheath surrounding the
vascular tissue and the epidermal region was found to have
the highest spin density and T1. Both tissues are comprised
of relatively small thick-walled cells. Cortical and pith
parenchyma are composed of larger, thinner-walled cells with
numerous intercellular spaces and lower spin density and T1.
The differences are attributed to the higher water content
by volume in the tissue composed of smaller, more compactly
arranged cells. The resolution obtained in this work enables
clear definition of tissues in the living plant and
quantitative information concerning differences in the
distribution and extent of binding of water.},
Doi = {10.1073/pnas.84.9.2752},
Key = {Johnson87d}
}
@article{fds268758,
Author = {Johnson, GA and Maronpot, RR},
Title = {Magnetic resonance microscopy of chemically-induced liver
foci.},
Journal = {Toxicol Pathol},
Volume = {17},
Number = {4 Pt 1},
Pages = {613-616},
Year = {1989},
ISSN = {0192-6233},
url = {http://www.ncbi.nlm.nih.gov/pubmed/2629098},
Keywords = {Animals • Carcinogenicity Tests • Diet •
Female • Liver • Liver Neoplasms, Experimental
• Magnetic Resonance Imaging • Rats • Rats,
Inbred F344 • chemically induced • pathology*
• ultrastructure},
Abstract = {Magnetic resonance imaging (MRI) is a new imaging technique
used in clinical diagnosis. This paper describes extension
of the technique to basic research applications--specifically
detecting and characterizing chemically-induced liver
neoplasms and foci of cellular alteration. Two systems have
been built that allow spatial microscopic resolution--more
than 100,000 x greater than that of earlier efforts. Use of
spin-lattice (T1) and spin-spin (T2) relaxation times
permits detailed characterization of the
tissue.},
Doi = {10.1177/0192623389017004106},
Key = {fds268758}
}
@article{fds132781,
Author = {EL Effmann and GA Johnson and BR Smith and GA Talbott and G
Cofer},
Title = {Magnetic resonance microscopy of chick embryos in
ovo.},
Journal = {Teratology, UNITED STATES},
Volume = {38},
Number = {1},
Pages = {59-65},
Year = {1988},
Month = {July},
ISSN = {0040-3709},
Keywords = {Animals • Cardiovascular System • Chick Embryo
• Magnetic Resonance Imaging • Nervous System
• anatomy & histology* • embryology •
methods*},
Abstract = {Magnetic resonance imaging (MRI) of the live 11-day chick
embryo with special radiofrequency coils and 3-D imaging
methods has produced contiguous 1.25-mm-thick slices with
200-microns pixel resolution, permitting definition of
cardiac chambers, cerebral ventricles, spinal cord, liver,
and lungs. It was the objective of this study to image
younger chick embryos in ovo with higher spatial resolution
through the application of implanted radiofrequency coils.
Fertilized Arbor Acre eggs were windowed at 9, 6, and 4
days. Circular coils 18 mm in diameter tuned to 85.5 MHz
were suspended around the developing embryo. The eggs were
sealed with tape and maintained at 37 degrees C during the
imaging procedure. MRI was performed in a 2.0-Tesla GE
system utilizing a 3-D Fourier transform acquisition in
sagittal and axial planes with a partial saturation sequence
(TR = 400 ms, TE = 27 ms). Approximately 1 hour of imaging
time was required to obtain 16 contiguous 600-microns-thick
slices with 50-microns pixel resolution. Embryos remained
viable through the imaging procedure. Embryos were
photographed, fixed, and cleared for correlative anatomical
study. Vitelline vessels, dorsal aorta, aortic arches,
cardinal veins, and cardiac chambers were identified as
areas of decreased signal intensity. Cerebral ventricles and
the vitreous portion of the eye have signal intensities that
are less than adjacent neural, scleral, and lens tissue.
Further refinements in MR instrumentation and imaging
sequences promise improvements in resolution and offer the
potential for sequential observations of the intact
embryo.},
Key = {fds132781}
}
@booklet{Effmann88,
Author = {Effmann, EL and Johnson, GA and Smith, BR and Talbott, GA and Cofer,
G},
Title = {Magnetic resonance microscopy of chick embryos in
ovo.},
Journal = {Teratology},
Volume = {38},
Number = {1},
Pages = {59-65},
Year = {1988},
Month = {July},
ISSN = {0040-3709},
url = {http://www.ncbi.nlm.nih.gov/pubmed/3175940},
Abstract = {Magnetic resonance imaging (MRI) of the live 11-day chick
embryo with special radiofrequency coils and 3-D imaging
methods has produced contiguous 1.25-mm-thick slices with
200-microns pixel resolution, permitting definition of
cardiac chambers, cerebral ventricles, spinal cord, liver,
and lungs. It was the objective of this study to image
younger chick embryos in ovo with higher spatial resolution
through the application of implanted radiofrequency coils.
Fertilized Arbor Acre eggs were windowed at 9, 6, and 4
days. Circular coils 18 mm in diameter tuned to 85.5 MHz
were suspended around the developing embryo. The eggs were
sealed with tape and maintained at 37 degrees C during the
imaging procedure. MRI was performed in a 2.0-Tesla GE
system utilizing a 3-D Fourier transform acquisition in
sagittal and axial planes with a partial saturation sequence
(TR = 400 ms, TE = 27 ms). Approximately 1 hour of imaging
time was required to obtain 16 contiguous 600-microns-thick
slices with 50-microns pixel resolution. Embryos remained
viable through the imaging procedure. Embryos were
photographed, fixed, and cleared for correlative anatomical
study. Vitelline vessels, dorsal aorta, aortic arches,
cardinal veins, and cardiac chambers were identified as
areas of decreased signal intensity. Cerebral ventricles and
the vitreous portion of the eye have signal intensities that
are less than adjacent neural, scleral, and lens tissue.
Further refinements in MR instrumentation and imaging
sequences promise improvements in resolution and offer the
potential for sequential observations of the intact
embryo.},
Doi = {10.1002/tera.1420380109},
Key = {Effmann88}
}
@booklet{Smith96,
Author = {Smith, BR and Linney, E and Huff, DS and Johnson,
GA},
Title = {Magnetic resonance microscopy of embryos.},
Journal = {Comput Med Imaging Graph},
Volume = {20},
Number = {6},
Pages = {483-490},
Year = {1996},
ISSN = {0895-6111},
url = {http://www.ncbi.nlm.nih.gov/pubmed/9007215},
Abstract = {We demonstrate that magnetic resonance (MR) microscopy
provides a mechanism to investigate normal and abnormal
developmental anatomy in a non-destructive and
distortion-free manner. Techniques for the fixation,
embedding, perfusion and image acquisition of embryos
between 3 and 30 mm crown rump length are described. We
describe the perfusion of a contrast agent to enhance images
of the developing embryonic vasculature. Data are acquired
as three-dimensional isotropic arrays which permit images to
be reformatted retrospectively in any plane. The data are
available for archiving, distributing and for
post-acquisition manipulations. MR microscopy is a fast
technique for producing three-dimensional reconstructions
and is free from registration and sectioning
artifacts.},
Doi = {10.1016/s0895-6111(96)00046-8},
Key = {Smith96}
}
@article{fds132819,
Author = {BR Smith and E Linney and DS Huff and GA Johnson},
Title = {Magnetic resonance microscopy of embryos.},
Journal = {Computerized medical imaging and graphics : the official
journal of the Computerized Medical Imaging Society, UNITED
STATES},
Volume = {20},
Number = {6},
Pages = {483-90},
ISSN = {0895-6111},
Keywords = {Anatomy, Artistic • Animals • Contrast Media
• Databases, Factual • Embryo • Embryonic and
Fetal Development* • Humans • Image Processing,
Computer-Assisted • Magnetic Resonance Imaging* •
Medical Illustration • Mice • Microscopy •
anatomy & histology • anatomy & histology* •
embryology},
Abstract = {We demonstrate that magnetic resonance (MR) microscopy
provides a mechanism to investigate normal and abnormal
developmental anatomy in a non-destructive and
distortion-free manner. Techniques for the fixation,
embedding, perfusion and image acquisition of embryos
between 3 and 30 mm crown rump length are described. We
describe the perfusion of a contrast agent to enhance images
of the developing embryonic vasculature. Data are acquired
as three-dimensional isotropic arrays which permit images to
be reformatted retrospectively in any plane. The data are
available for archiving, distributing and for
post-acquisition manipulations. MR microscopy is a fast
technique for producing three-dimensional reconstructions
and is free from registration and sectioning
artifacts.},
Key = {fds132819}
}
@booklet{Smith94,
Author = {Smith, BR and Johnson, GA and Groman, EV and Linney,
E},
Title = {Magnetic resonance microscopy of mouse embryos.},
Journal = {Proc Natl Acad Sci U S A},
Volume = {91},
Number = {9},
Pages = {3530-3533},
Year = {1994},
Month = {April},
ISSN = {0027-8424},
url = {http://www.ncbi.nlm.nih.gov/pubmed/8170941},
Abstract = {The increased use of the mouse as a model for various
aspects of mammalian biology has caused a renewed interest
in developing strategies for examining and comparing normal
and abnormal mouse embryonic development and anatomy. In
this study, we have explored the use of magnetic resonance
microscopy as a tool for these purposes. Techniques for the
fixation, embedding, perfusion, and image acquisition of
mouse embryos are described. The perfusion of bovine serum
albumin-diethylenetriamine pentaacetic anhydride-gadolinium
as a contrast agent enhances images of the developing
embryonic vasculature during critical stages of
organogenesis and allows for comparisons when embryos have
been treated with teratogens such as retinoic acid. The
acquired three-dimensional data sets are available for
archiving, distributing, and postacquisition manipulations
such as computer segmentation of anatomical
structures.},
Doi = {10.1073/pnas.91.9.3530},
Key = {Smith94}
}
@article{fds132863,
Author = {BR Smith and GA Johnson and EV Groman and E Linney},
Title = {Magnetic resonance microscopy of mouse embryos.},
Journal = {Proceedings of the National Academy of Sciences of the
United States of America, UNITED STATES},
Volume = {91},
Number = {9},
Pages = {3530-3},
Year = {1994},
Month = {April},
ISSN = {0027-8424},
Keywords = {Age Factors • Albumins • Animals • Contrast
Media • Gadolinium DTPA* • Image Processing,
Computer-Assisted • Mice • Organometallic
Compounds • Pentetic Acid • analogs & derivatives
• diagnostic use • embryology*},
Abstract = {The increased use of the mouse as a model for various
aspects of mammalian biology has caused a renewed interest
in developing strategies for examining and comparing normal
and abnormal mouse embryonic development and anatomy. In
this study, we have explored the use of magnetic resonance
microscopy as a tool for these purposes. Techniques for the
fixation, embedding, perfusion, and image acquisition of
mouse embryos are described. The perfusion of bovine serum
albumin-diethylenetriamine pentaacetic anhydride-gadolinium
as a contrast agent enhances images of the developing
embryonic vasculature during critical stages of
organogenesis and allows for comparisons when embryos have
been treated with teratogens such as retinoic acid. The
acquired three-dimensional data sets are available for
archiving, distributing, and postacquisition manipulations
such as computer segmentation of anatomical
structures.},
Key = {fds132863}
}
@booklet{Brown88,
Author = {Brown, JM and Thomas, JF and Cofer, GP and Johnson,
GA},
Title = {Magnetic Resonance Microscopy of Stem Tissues of Pelargonium
hortorum},
Journal = {Botanical Gazette},
Volume = {149},
Number = {3},
Pages = {253-259},
Publisher = {University of Chicago Press},
Year = {1988},
Month = {September},
ISSN = {0006-8071},
url = {http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:A1988Q941300001&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=47d3190e77e5a3a53558812f597b0b92},
Doi = {10.1086/337713},
Key = {Brown88}
}
@booklet{Benveniste00,
Author = {Benveniste, H and Kim, K and Zhang, L and Johnson,
GA},
Title = {Magnetic resonance microscopy of the C57BL mouse
brain.},
Journal = {Neuroimage},
Volume = {11},
Number = {6 Pt 1},
Pages = {601-611},
Year = {2000},
Month = {June},
ISSN = {1053-8119},
url = {http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000087963600003&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=47d3190e77e5a3a53558812f597b0b92},
Abstract = {With the rapid progression in gene technologies, transgenic,
targeted, and chemically induced mutations in mice are
continually created. The major goal of these studies is to
understand and characterize the effects of genotype on
anatomy, physiology, and behavior and ultimately the role of
genotype in development of disease. The demand for imaging
techniques with high spatial resolution potential is rising
because such imaging tools would expedite anatomical
phenotyping in the genetically altered mice. Magnetic
resonance microscopy (MRM) is a noninvasive, inherently
three-dimensional (3D) imaging technique capable of
visualizing several anatomical structures in the small
mouse. The 3D nature of MRM also allows for interpretation
of complex spatial relationships between substructures,
which is important when phenotyping anatomically. The goal
of this paper is to systematically describe three major
brain regions in the C57BL/6J mouse at microanatomical
spatial resolution ranges using in vitro MRM. We explore
different MR contrast parameters, voxel sizes, and
signal-to-noise ratios to best characterize C57BL/6J mouse
brain microstructure by MRM. Further, we compare all MRM
images with Nissl-stained brain sections. Major findings
were as follows: T2* MR images visualized several gross
anatomical regions in the mouse brain but not, for example,
subregions within the hippocampus. Diffusion proton stains
on the other hand were superior to T2* MR images and
delineated many subregions within the hippocampus proper.
Finally, contrast enhancement facilitated visualization of
hippocampal anatomy on the T2* MR images. The results of
this study are part of an ongoing initiative at our Center
focused on creating a complete C57BL/6J mouse anatomical 3D
image database by MRM.},
Doi = {10.1006/nimg.2000.0567},
Key = {Benveniste00}
}
@article{fds268911,
Author = {Arnder, L and Zhou, X and Cofer, GP and Hedlund, LW and Johnson,
GA},
Title = {Magnetic resonance microscopy of the rat carotid artery at
300 megahertz.},
Journal = {Invest Radiol},
Volume = {29},
Number = {9},
Pages = {822-826},
Year = {1994},
Month = {September},
ISSN = {0020-9996},
url = {http://www.ncbi.nlm.nih.gov/pubmed/7995700},
Keywords = {Angioplasty, Balloon • Animals • Arteriosclerosis
• Carotid Arteries • Carotid Artery Diseases
• Female • Magnetic Resonance Angiography •
Microscopy • Rats • Rats, Sprague-Dawley •
diagnosis • methods* • pathology* •
therapy},
Abstract = {RATIONALE AND OBJECTIVES: Magnetic resonance microscopy
(MRM) has evolved from a technical curiosity to a tool with
which researchers can study important disease models. But
MRM is not simply an extension of clinical magnetic
resonance imaging. In this article, the unique adaptations
of MRM required in the study of carotid artery disease are
outlined. The techniques of MRM are integrated into a
specific model of carotid artery disease in the rat to allow
in vivo studies of vascular wall thickening after removal of
the vascular endothelium. METHODS: Imaging was performed at
300 MHz in 250-gm Sprague-Dawley rats using surgically
implanted radiofrequency coils to localize the region of
interest and to provide an increase in the signal-to-noise
ratio over that of volume or surface coils. A
three-dimensional Fourier encoding sequence was modified
with flow-dephasing gradients to minimize signal and
artifacts from moving blood. RESULTS: In vivo images were
acquired with spatial resolution of 25 x 25 x 400 microns
and signal-to-noise ratio more than sufficient to define the
morphology of the vascular wall. Significant changes in the
intensity and distribution of signal were visible in the
area surrounding the vessel after angioplasty. CONCLUSIONS:
Signal-to-noise improvements from surgically implanted coils
coupled to a three-dimensional radiofrequency-refocused
sequence with flow-dephasing gradients were sufficient to
define the wall of the carotid artery. The
diffusion-weighted pulse sequence detects signal changes in
the area surrounding the vessel after angioplasty. The MRM
techniques described and the contrast observed allow us, for
the first time to follow in vivo the early stage of
developing atherosclerosis in the vessel wall and closely
surrounding tissue.},
Doi = {10.1097/00004424-199409000-00004},
Key = {fds268911}
}
@article{fds268993,
Author = {Hedlund, LW and Johnson, GA and Mills, GI},
Title = {Magnetic resonance microscopy of the rat thorax and
abdomen.},
Journal = {Invest Radiol},
Volume = {21},
Number = {11},
Pages = {843-846},
Year = {1986},
Month = {November},
ISSN = {0020-9996},
url = {http://www.ncbi.nlm.nih.gov/pubmed/3781788},
Keywords = {Abdomen • Animals • Magnetic Resonance
Spectroscopy • Male • Myocardial Contraction
• Rats • Rats, Inbred Strains • Respiration
• Thorax • anatomy & histology* • diagnostic
use},
Abstract = {With magnetic resonance (MR) microscopy, high-resolution
volumetric imaging (3DFT) of small animals is possible.
Although these techniques are suitable for imaging the head
and other small stationary objects, breathing and cardiac
motion degrade the quality of body images. Scan synchronous
ventilation and cardiac gating methods have been developed
that permit acquisition of high-resolution images from
anywhere in the body of small animals (150 to 400 g).
Anesthetized rats were ventilated in synchrony with
three-dimensional Fourier spin warp (3DFT) sequence (TR =
400 to 1000 ms, TE = 20 ms). Eight or 16 slices (1.2 or 2.5
mm thick) were acquired simultaneously. Effective pixel size
was 200 X 200 mu. Imaging was performed in a 1.5 T, 1-m bore
research system using a 28-cm diameter high field gradient
coil and a 6-cm diameter radio frequency coil. For thoracic
imaging, acquisitions were gated to the QRS of the ECG. Scan
synchronous ventilation eliminated breathing motion
artifacts and permitted visualization of peripheral vascular
structures in the lung and liver. In images that were also
cardiac gated, cardiac chambers and major thoracic vessels,
including the coronary arteries, were well demonstrated.
Thus, thoroughly characterized rodent models can now be
studied with MR not only to explore noninvasively the
intricacies of mammalian pathomorphology, but also to test
the capabilities of MR and aid in interpreting MR
data.},
Doi = {10.1097/00004424-198611000-00002},
Key = {fds268993}
}
@booklet{Hedlund91,
Author = {Hedlund, LW and Maronpot, RR and Johnson, GA and Cofer, GP and Mills,
GI and Wheeler, CT},
Title = {Magnetic resonance microscopy of toxic renal injury induced
by Bromoethylamine in rats},
Journal = {Toxicological Sciences},
Volume = {16},
Number = {4},
Pages = {787-797},
Publisher = {Oxford University Press (OUP)},
Year = {1991},
Month = {January},
ISSN = {0272-0590},
url = {http://dx.doi.org/10.1093/toxsci/16.4.787},
Abstract = {Magnetic Resonance Microscopy of Toxic Renal Injury Induced
by Bromoethylamine in Rats. Hedlund, L. W., Maronpot, R. R.,
Johnson, G. A., Cofer, G. P., Mills, G. I., and Wheeler, C.
T. (1991). Fundam. Appl. Toxicol. 16, 787-797. The
alkylhalide 2-bromoethylamine hydro-bromide (BEA) produces
renal injury in rats that mimics analgesic-related renal
injury in humans. Our purpose was to examine this injury, in
vivo in rats, with magnetic resonance (MR) microscopy and
correlate MR findings with findings from light microscopy of
hematoxylin-eosin-stained sections. Rats (n = 48) were
injected intravenously with BEA (150 mg/kg) or saline and
imaged with MR 6, 48, and 336 hr later. The spin-spin
relaxation time, T2, was measured from the cortex to the
papilla. In other rats, we measured regional water content
of the kidney. Renal injury was present 48 and 336 hr after
BEA dosing based on increased renal organ weights, decreased
urine specific gravity, and significant renal lesions (H &
E). T2 was elevated in the inner stripe of the outer medulla
in injured kidneys at 48 hr. The differences in T2 between
cortex and outer medulla were also elevated 48 hr after BEA.
In the inner medulla, there were no changes in T2 after BEA
treatment. However, in all groups there were significant
regional differences in T2. The value of T2 increased from
outer to inner medulla and this gradient was directly
correlated with water content. Thus, MR microscopy detected
damage in the outer medulla after BEA injury but not the
damage in the inner medulla. T2 appeared to reflect the
water content in the different regions of the medulla. The
noninvasive in vivo capability of MR microscopy, with its
high sensitivity to tissue water, allows the toxicologist to
monitor the progression and regression of toxic insult in
the same animal. At present the technology is complicated.
The precise and accurate measure of MR-sensitive parameters
in live animals at microscopic resolution is difficult.
However, as the technology matures, there will be
significant improvements providing the toxicologist a unique
in vivo tool. © 1991 by the Society of Toxicology.},
Doi = {10.1093/toxsci/16.4.787},
Key = {Hedlund91}
}
@article{fds269102,
Author = {Hedlund, LW and Maronpot, RR and Johnson, GA and Cofer, GP and Mills,
GI and Wheeler, CT},
Title = {Magnetic resonance microscopy of toxic renal injury induced
by bromoethylamine in rats.},
Journal = {Fundam Appl Toxicol},
Volume = {16},
Number = {4},
Pages = {787-797},
Year = {1991},
Month = {May},
ISSN = {0272-0590},
url = {http://www.ncbi.nlm.nih.gov/pubmed/1884916},
Keywords = {Animals • Ethylamines • Female • Kidney
• Kidney Cortex • Kidney Diseases • Kidney
Medulla • Magnetic Resonance Spectroscopy* •
Microscopy • Organ Size • Rats • Rats, Inbred
Strains • Specific Gravity • chemically induced*
• methods* • pathology • pathology* •
toxicity*},
Abstract = {The alkylhalide 2-bromoethylamine hydrobromide (BEA)
produces renal injury in rats that mimics analgesic-related
renal injury in humans. Our purpose was to examine this
injury, in vivo in rats, with magnetic resonance (MR)
microscopy and correlate MR findings with findings from
light microscopy of hematoxylin-eosin-stained sections. Rats
(n = 48) were injected intravenously with BEA (150 mg/kg) or
saline and imaged with MR 6, 48, and 336 hr later. The
spin-spin relaxation time, T2, was measured from the cortex
to the papilla. In other rats, we measured regional water
content of the kidney. Renal injury was present 48 and 336
hr after BEA dosing based on increased renal organ weights,
decreased urine specific gravity, and significant renal
lesions (H & E). T2 was elevated in the inner stripe of the
outer medulla in injured kidneys at 48 hr. The differences
in T2 between cortex and outer medulla were also elevated 48
hr after BEA. In the inner medulla, there were no changes in
T2 after BEA treatment. However, in all groups there were
significant regional differences in T2. The value of T2
increased from outer to inner medulla and this gradient was
directly correlated with water content. Thus, MR microscopy
detected damage in the outer medulla after BEA injury but
not the damage in the inner medulla. T2 appeared to reflect
the water content in the different regions of the medulla.
The noninvasive in vivo capability of MR microscopy, with
its high sensitivity to tissue water, allows the
toxicologist to monitor the progression and regression of
toxic insult in the same animal. At present the technology
is complicated. The precise and accurate measure of
MR-sensitive parameters in live animals at microscopic
resolution is difficult. However, as the technology matures,
there will be significant improvements providing the
toxicologist a unique in vivo tool.},
Doi = {10.1016/0272-0590(91)90164-y},
Key = {fds269102}
}
@booklet{Tengowski00,
Author = {Tengowski, MW and Hedlund, LW and Guyot, DJ and Burkhardt, JE and Johnson, GA},
Title = {Magnetic resonance microscopy predicts findings in a
theophylline-induced rat model of reproductive
toxicity},
Journal = {MOLECULAR BIOLOGY OF THE CELL},
Volume = {11},
Pages = {125A-125A},
Publisher = {AMER SOC CELL BIOLOGY},
Year = {2000},
Month = {December},
ISSN = {1059-1524},
url = {http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000165525900655&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=47d3190e77e5a3a53558812f597b0b92},
Key = {Tengowski00}
}
@booklet{Delnomdedieu96,
Author = {Delnomdedieu, M and Hedlund, LW and Johnson, GA and Maronpot,
RR},
Title = {Magnetic resonance microscopy--a new tool for the
toxicologic pathologist.},
Journal = {Toxicol Pathol},
Volume = {24},
Number = {1},
Pages = {36-44},
Year = {1996},
ISSN = {0192-6233},
url = {http://www.ncbi.nlm.nih.gov/pubmed/8839279},
Abstract = {Parallel to its many applications in medical imaging,
magnetic resonance (MR) microscopy is a potentially powerful
tool in toxicologic pathology. Because of the intrinsic
qualities of MR microscopy (noninvasiveness,
3-dimensionality, and slicing in any chosen plane), the
scientist has a new means by which to investigate different
types of lesions based on differential contrast. By choosing
appropriate proton stains to probe the state of the water in
tissues, organ structure and vasculature can be seen and
progressive lesion development can be followed in a given
animal. This paper discusses toxicologic pathology
applications for MR microscopy and compares MR microscopy
with conventional histopathology using a time-course study
of bromobenzene-induced hepatotoxicity in rats. Hematoxylin
and eosin (H&E)-stained histological sections are compared
with MR microscopy images from fixed tissue blocks to
demonstrate one of the applications of MR microscopy to
toxicologic pathology. The results indicate that MR
microscopy is as sensitive as conventional H&E staining in
detecting bromobenzene-induced hepatic lesions.},
Doi = {10.1177/019262339602400106},
Key = {Delnomdedieu96}
}
@article{fds132841,
Author = {M Delnomdedieu and LW Hedlund and GA Johnson and RR
Maronpot},
Title = {Magnetic resonance microscopy--a new tool for the
toxicologic pathologist.},
Journal = {Toxicologic pathology, UNITED STATES},
Volume = {24},
Number = {1},
Pages = {36-44},
ISSN = {0192-6233},
Keywords = {Animals • Brain Ischemia • Disease Models, Animal
• Kidney • Liver • Magnetic Resonance Imaging
• Microscopy • Rats • blood supply •
drug effects • methods* • pathology •
pathology*},
Abstract = {Parallel to its many applications in medical imaging,
magnetic resonance (MR) microscopy is a potentially powerful
tool in toxicologic pathology. Because of the intrinsic
qualities of MR microscopy (noninvasiveness,
3-dimensionality, and slicing in any chosen plane), the
scientist has a new means by which to investigate different
types of lesions based on differential contrast. By choosing
appropriate proton stains to probe the state of the water in
tissues, organ structure and vasculature can be seen and
progressive lesion development can be followed in a given
animal. This paper discusses toxicologic pathology
applications for MR microscopy and compares MR microscopy
with conventional histopathology using a time-course study
of bromobenzene-induced hepatotoxicity in rats. Hematoxylin
and eosin (H&E)-stained histological sections are compared
with MR microscopy images from fixed tissue blocks to
demonstrate one of the applications of MR microscopy to
toxicologic pathology. The results indicate that MR
microscopy is as sensitive as conventional H&E staining in
detecting bromobenzene-induced hepatic lesions.},
Key = {fds132841}
}
@article{fds325753,
Author = {Lipinski, RJ and Hammond, P and Ament, JJ and Pecevich, SJ and Jiang, Y and Dehart, DB and Johnson, GA and Sulik, KK},
Title = {MAGNETIC RESONANCE MICROSCOPY-BASED 3D FACE-BRAIN
CORRELATIONS IN AN FASD MOUSE MODEL},
Journal = {ALCOHOLISM-CLINICAL AND EXPERIMENTAL RESEARCH},
Volume = {35},
Number = {6},
Pages = {266A-266A},
Year = {2011},
Key = {fds325753}
}
@article{fds268826,
Author = {O'Leary-Moore, SK and Parnell, SE and Godin, EA and Dehart, DB and Ament, JJ and Khan, AA and Johnson, GA and Styner, MA and Sulik,
KK},
Title = {Magnetic resonance microscopy-based analyses of the brains
of normal and ethanol-exposed fetal mice.},
Journal = {Birth Defects Res A Clin Mol Teratol},
Volume = {88},
Number = {11},
Pages = {953-964},
Year = {2010},
Month = {November},
ISSN = {1542-0760},
url = {http://www.ncbi.nlm.nih.gov/pubmed/20842647},
Keywords = {Abnormalities, Drug-Induced • Animals • Brain
• Disease Models, Animal • Ethanol • Female
• Fetal Alcohol Syndrome • Fetus •
Gestational Age • Humans • Magnetic Resonance
Imaging • Male • Mice • Mice, Inbred C57BL
• Microscopy • Pregnancy • Prenatal Exposure
Delayed Effects • abnormalities • chemically
induced • drug effects* • embryology •
methods • methods* • pathology •
toxicity*},
Abstract = {BACKGROUND: The application of magnetic resonance microscopy
(MRM) to the study of normal and abnormal prenatal mouse
development has facilitated discovery of dysmorphology
following prenatal ethanol insult. The current analyses
extend this work, providing a regional brain volume-based
description of normal brain growth and illustrating the
consequences of gestational day (GD) 10 ethanol exposure in
the fetal mouse. METHODS: To assess normal growth, control
C57Bl/6J fetuses collected on GD 16, GD 16.5, and GD 17 were
scanned using a 9.4-T magnet, resulting in 29-μm isotropic
resolution images. For the ethanol teratogenicity studies,
C57Bl/6J dams were administered intraperitoneal ethanol (2.9
g/kg) at 10 days, 0 hr, and 10 days, 4 hr, after
fertilization, and fetuses were collected for analyses on GD
17. From individual MRM scans, linear measurements and
regional brain volumes were determined and compared.
RESULTS: In control fetuses, each of the assessed brain
regions increased in volume, whereas ventricular volumes
decreased between GD 16 and GD 17. Illustrating a global
developmental delay, prenatal ethanol exposure resulted in
reduced body volumes, crown-rump lengths, and a generalized
decrease in regional brain volumes compared with GD 17
controls. However, compared with GD 16.5, morphologically
matched controls, ethanol exposure resulted in volume
increases in the lateral and third ventricles as well as a
disproportionate reduction in cortical volume. CONCLUSIONS:
The normative data collected in this study facilitate the
distinction between GD 10 ethanol-induced developmental
delay and frank dysmorphology. This work illustrates the
utility of MRM-based analyses for developmental toxicology
studies and extends our knowledge of the stage-dependency of
ethanol teratogenesis.},
Language = {eng},
Doi = {10.1002/bdra.20719},
Key = {fds268826}
}
@article{fds268727,
Author = {Parnell, SE and Holloway, HT and O'Leary-Moore, SK and Dehart, DB and Paniaqua, B and Oguz, I and Budin, F and Styner, MA and Johnson, GA and Sulik, KK},
Title = {Magnetic resonance microscopy-based analyses of the
neuroanatomical effects of gestational day 9 ethanol
exposure in mice.},
Journal = {Neurotoxicol Teratol},
Volume = {39},
Pages = {77-83},
Year = {2013},
ISSN = {0892-0362},
url = {http://dx.doi.org/10.1016/j.ntt.2013.07.009},
Abstract = {Animal model-based studies have shown that ethanol exposure
during early gestation induces developmental stage-specific
abnormalities of the face and brain. The exposure
time-dependent variability in ethanol's teratogenic outcomes
is expected to contribute significantly to the wide spectrum
of effects observed in humans with fetal alcohol spectrum
disorder (FASD). The work presented here employs a mouse
FASD model and magnetic resonance microscopy (MRM; high
resolution magnetic resonance imaging) in studies designed
to further our understanding of the developmental
stage-specific defects of the brain that are induced by
ethanol. At neurulation stages, i.e. at the beginning of
gestational day (GD) 9 and again 4 hours later, time-mated
C57Bl/6J dams were intraperitoneally administered 2.9 g/kg
ethanol or vehicle. Ethanol-exposed fetuses were collected
on GD 17, processed for MRM analysis, and results compared
to comparably staged controls. Linear and volume
measurements as well as shape changes for numerous
individual brain regions were determined. GD 9 ethanol
exposure resulted in significantly increased septal region
width, reduction of cerebellar volume, and enlargement of
all of the ventricles. Additionally, the results of shape
analyses showed that many areas of the ethanol-exposed
brains including the cerebral cortex, hippocampus and right
striatum were significantly misshapen. These data
demonstrate that ethanol can induce dysmorphology that may
not be obvious based on volumetric analyses alone, highlight
the asymmetric aspects of ethanol-induced defects, and add
to our understanding of ethanol's developmental
stage-dependent neuroteratogenesis.},
Doi = {10.1016/j.ntt.2013.07.009},
Key = {fds268727}
}
@article{fds304894,
Author = {Badea, A and Johnson, GA},
Title = {Magnetic resonance microscopy.},
Journal = {Stud Health Technol Inform},
Volume = {185},
Pages = {153-184},
Year = {2013},
ISSN = {0926-9630},
url = {http://www.ncbi.nlm.nih.gov/pubmed/23542935},
Abstract = {MRI, one of the major clinical imaging modalities, has
gained an important role in studying small animal models,
e.g., rats and mice. But imaging rodents comes with
challenges, since the image resolution needs to be ~
3000-times higher to resolve anatomical details at a level
comparable to clinical imaging. A resolution on the order of
100 microns or less redefines MR imaging as MR microscopy.
We discuss in this chapter the basic components of the MR
imaging chain, with a particular emphasis on small animal
imaging demands: from hardware design to basic physical
principles of MR image formation, and contrast mechanisms.
We discuss special considerations of animal preparation for
imaging, and staining methods to enhance contrast. Attention
is given to factors that increase sensitivity, including
exogenous contrast agents, high performance radiofrequency
detectors, and advanced MR encoding sequences. Among these,
diffusion tensor imaging and tractography add novel
information on white matter tracts, helping to better
understand important aspects of development and
neurodegeneration. These developments open avenues for
efficient phenotyping of small animal models, in vivo - to
include anatomical as well as functional estimates, or
ex-vivo - with exquisite anatomical detail. The need for
higher resolution results in larger image arrays that need
to be processed efficiently. We discuss image-processing
approaches for quantitative characterization of animal
cohorts, and building population atlases. High throughput is
essential for these methods to become practical. We discuss
current trends for increasing detector performance, the use
of cryoprobes, as well as strategies for imaging multiple
animals at the same time. Ultimately, the development of
highly specific probes, with the possibility to be used in
multimodal imaging, will offer new insights into histology.
MRM, alone or in combination with other imaging modalities,
will increase the knowledge of fundamental biological
processes, help understanding the genetic basis of human
diseases, and test pharmacological interventions.},
Key = {fds304894}
}
@article{fds268793,
Author = {Badea, A and Johnson, GA},
Title = {Magnetic resonance microscopy.},
Journal = {Anal Cell Pathol (Amst)},
Volume = {35},
Number = {4},
Pages = {205-227},
Booktitle = {Medical Imaging: Principles and Practices},
Publisher = {IOS Press Ebooks},
Year = {2012},
ISBN = {9781439871027},
ISSN = {0926-9630},
url = {http://www.ncbi.nlm.nih.gov/pubmed/22142643},
Abstract = {Visualization of internal structures of opaque biologic
objects is essential in many biomedical studies. Limited by
the penetration depth of the probing sources (photons and
electrons) and the lack of endogenous contrast, conventional
forms of microscopy such as optical microscopy and electron
microscopy require tissues to be sectioned into thin slices
and stained with organic chemicals or heavy-metal compounds
prior to examination. These invasive and destructive
procedures, as well as the harmful radiation in the case of
electron microscopy, make it difficult to obtain
three-dimensional information and virtually impossible to
study biologic tissues in vivo.},
Doi = {10.3233/ACP-2011-0050},
Key = {fds268793}
}
@booklet{Conner88,
Author = {Conner, WE and Johnson, GA and Cofer, GP and Dittrich,
K},
Title = {Magnetic resonance microscopy: in vivo sectioning of a
developing insect.},
Journal = {Experientia},
Volume = {44},
Number = {1},
Pages = {11-12},
Year = {1988},
Month = {January},
ISSN = {0014-4754},
url = {http://www.ncbi.nlm.nih.gov/pubmed/3350110},
Abstract = {The utility of magnetic resonance imaging vis-a-vis insect
morphology and development was investigated. MRI is a
noninvasive technique that distinguishes between tissues
based on proton content and proton 'environment'. At present
a resolution of 100 micron is achievable. The technique
avoids fixation artifacts and allows the detection of motion
within the organism.},
Doi = {10.1007/BF01960223},
Key = {Conner88}
}
@article{fds132771,
Author = {WE Conner and GA Johnson and GP Cofer and K Dittrich},
Title = {Magnetic resonance microscopy: in vivo sectioning of a
developing insect.},
Journal = {Experientia, SWITZERLAND},
Volume = {44},
Number = {1},
Pages = {11-2},
Year = {1988},
Month = {January},
ISSN = {0014-4754},
Keywords = {Animals • Female • Lepidoptera • Magnetic
Resonance Imaging* • Microscopy • Moths •
Pupa • anatomy & histology • anatomy & histology*
• growth & development},
Abstract = {The utility of magnetic resonance imaging vis-a-vis insect
morphology and development was investigated. MRI is a
noninvasive technique that distinguishes between tissues
based on proton content and proton 'environment'. At present
a resolution of 100 micron is achievable. The technique
avoids fixation artifacts and allows the detection of motion
within the organism.},
Key = {fds132771}
}
@article{fds268748,
Author = {MACFALL, JS and SPAINE, PC and DOUDRICK, RE and JOHNSON,
GA},
Title = {MAGNETIC-RESONANCE MICROSCOPY (MRM) OF WATER TRANSPORT AND
BINDING IN FUSIFORM RUST GALLS},
Journal = {RESEARCH AND APPLICATIONS OF CHEMICAL SCIENCES IN
FORESTRY},
Volume = {104},
Pages = {17-17},
Publisher = {US DEPT AGR, FOREST SERV SO FOREST EXPT STN},
Editor = {Vozzo, JA},
Year = {1994},
Month = {January},
url = {http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:A1994BA75T00004&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=47d3190e77e5a3a53558812f597b0b92},
Key = {fds268748}
}
@booklet{Effmann87,
Author = {EFFMANN, EL and JOHNSON, GA and SMITH, BR and TALBOTT, GA and COFER,
G},
Title = {MAGNETIC-RESONANCE MICROSCOPY OF LIVE CHICK-EMBRYOS IN
OVO},
Journal = {TERATOLOGY},
Volume = {35},
Number = {2},
Pages = {A44-A44},
Publisher = {WILEY-LISS},
Year = {1987},
Month = {April},
ISSN = {0040-3709},
url = {http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:A1987H226300076&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=47d3190e77e5a3a53558812f597b0b92},
Key = {Effmann87}
}
@booklet{Arnder94,
Author = {L. Arnder and X. H. Zhou and G. P. Cofer and L. W. Hedlund and G. A. Johnson},
Title = {Magnetic-resonance microscopy of the rat carotid-artery at
300-megahertz},
Journal = {Investigative Radiology},
Volume = {29},
Number = {9},
Pages = {822 -- 826},
Year = {1994},
Month = {September},
Key = {Arnder94}
}
@booklet{Hedlund86a,
Author = {L. W. Hedlund and G. A. Johnson and G. I.
Mills},
Title = {Magnetic-resonance microscopy of the rat thorax and
abdomen},
Journal = {Investigative Radiology},
Volume = {21},
Number = {11},
Pages = {843 -- 846},
Year = {1986},
Month = {November},
Key = {Hedlund86a}
}
@article{fds325757,
Author = {SPAINE, P and MACFALL, JS and JOHNSON, GA},
Title = {MAGNETIC-RESONANCE MICROSCOPY OF WATER-MOVEMENT THROUGH
FUSIFORM RUST GALLS OF PINE},
Journal = {RESEARCH AND APPLICATIONS OF CHEMICAL SCIENCES IN
FORESTRY},
Volume = {104},
Pages = {11-16},
Year = {1994},
Key = {fds325757}
}
@booklet{Drayer86a,
Author = {DRAYER, B and BURGER, P and DARWIN, R and RIEDERER, S and HERFKENS, R and JOHNSON, GA},
Title = {MAGNETIC-RESONANCE-IMAGING OF BRAIN IRON},
Journal = {AMERICAN JOURNAL OF NEURORADIOLOGY},
Volume = {7},
Number = {3},
Pages = {373-380},
Publisher = {AMER SOC NEURORADIOLOGY},
Year = {1986},
Month = {May},
ISSN = {0195-6108},
url = {http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:A1986C133300001&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=47d3190e77e5a3a53558812f597b0b92},
Key = {Drayer86a}
}
@booklet{Brown87a,
Author = {BROWN, JM and KRAMER, PJ and COFER, GP and JOHNSON,
GA},
Title = {MAGNETIC-RESONANCE-IMAGING OF ROOT-WATER
DISTRIBUTION},
Journal = {HORTSCIENCE},
Volume = {22},
Number = {5},
Pages = {1087-1087},
Publisher = {AMER SOC HORTICULTURAL SCIENCE},
Year = {1987},
Month = {October},
ISSN = {0018-5345},
url = {http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:A1987K430400370&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=47d3190e77e5a3a53558812f597b0b92},
Key = {Brown87a}
}
@article{fds357207,
Author = {Sitek, KR and Gulban, OF and Calabrese, E and Johnson, GA and Lage-Castellanos, A and Moerel, M and Ghosh, SS and De Martino,
F},
Title = {Mapping the human subcortical auditory system using
histology, postmortem MRI and in vivo MRI at
7T.},
Journal = {Elife},
Volume = {8},
Year = {2019},
Month = {August},
url = {http://dx.doi.org/10.7554/eLife.48932},
Abstract = {Studying the human subcortical auditory system
non-invasively is challenging due to its small, densely
packed structures deep within the brain. Additionally, the
elaborate three-dimensional (3-D) structure of the system
can be difficult to understand based on currently available
2-D schematics and animal models. Wfe addressed these issues
using a combination of histological data, post mortem
magnetic resonance imaging (MRI), and in vivo MRI at 7
Tesla. We created anatomical atlases based on
state-of-the-art human histology (BigBrain) and postmortem
MRI (50 µm). We measured functional MRI (fMRI) responses to
natural sounds and demonstrate that the functional
localization of subcortical structures is reliable within
individual participants who were scanned in two different
experiments. Further, a group functional atlas derived from
the functional data locates these structures with a median
distance below 2 mm. Using diffusion MRI tractography, we
revealed structural connectivity maps of the human
subcortical auditory pathway both in vivo (1050 µm
isotropic resolution) and post mortem (200 µm isotropic
resolution). This work captures current MRI capabilities for
investigating the human subcortical auditory system,
describes challenges that remain, and contributes novel,
openly available data, atlases, and tools for researching
the human auditory system.},
Doi = {10.7554/eLife.48932},
Key = {fds357207}
}
@article{fds357198,
Author = {Garrett, A and Rakhilin, N and Wang, N and McKey, J and Cofer, G and Anderson, RB and Capel, B and Johnson, GA and Shen,
X},
Title = {Mapping the peripheral nervous system in the whole mouse via
compressed sensing tractography.},
Journal = {J Neural Eng},
Volume = {18},
Number = {4},
Year = {2021},
Month = {June},
url = {http://dx.doi.org/10.1088/1741-2552/ac0089},
Abstract = {Objective.The peripheral nervous system (PNS) connects the
central nervous system with the rest of the body to regulate
many physiological functions and is therapeutically targeted
to treat diseases such as epilepsy, depression, intestinal
dysmotility, chronic pain, and more. However, we still lack
understanding of PNS innervation in most organs because the
large span, diffuse nature, and small terminal nerve bundle
fibers have precluded whole-organism, high resolution
mapping of the PNS. We sought to produce a comprehensive
peripheral nerve atlas for use in future interrogation of
neural circuitry and selection of targets for
neuromodulation.Approach.We used diffusion tensor magnetic
resonance imaging (DT-MRI) with high-speed compressed
sensing to generate a tractogram of the whole mouse PNS. The
tractography generated from the DT-MRI data is validated
using lightsheet microscopy on optically cleared, antibody
stained tissue.Main results.Herein we demonstrate the first
comprehensive PNS tractography in a whole mouse. Using this
technique, we scanned the whole mouse in 28 h and mapped PNS
innervation and fiber network in multiple organs including
heart, lung, liver, kidneys, stomach, intestines, and
bladder at 70µm resolution. This whole-body PNS
tractography map has provided unparalleled information; for
example, it delineates the innervation along the
gastrointestinal tract by multiple sacral levels and by the
vagal nerves. The map enabled a quantitative tractogram that
revealed relative innervation of the major organs by each
vertebral foramen as well as the vagus nerve.Significance.This
novel high-resolution nerve atlas provides a potential
roadmap for future neuromodulation therapies and other
investigations into the neural circuits which drive
homeostasis and disease throughout the body.},
Doi = {10.1088/1741-2552/ac0089},
Key = {fds357198}
}
@article{fds174254,
Author = {RD Geisert and JW Ross, MD Ashworth and FJ White and GA Johnson and U
DeSilva},
Title = {Maternal recognition of pregnancy signal or endocrine
disruptor: the two faces of oestrogen during establishment
of pregnancy in the pig.},
Journal = {Society of Reproduction and Fertility supplement},
Volume = {62},
Pages = {131-45},
Year = {2006},
Keywords = {Animals • Endocrine Disruptors • Endometrium
• Estrogens • Female • Pregnancy •
Pregnancy Maintenance • Pregnancy, Animal •
Receptors, Progesterone • Swine • metabolism
• metabolism* • physiology*},
Abstract = {Timing of conceptus growth and attachment to the uterine
luminal epithelium is regulated by progesterone secretion
from the corpus luteum and by expression of progesterone
receptor in the uterine epithelia and stroma. Conceptus
growth and uterine attachment are temporally associated with
the disappearance of progesterone receptors from uterine
epithelia. While the loss of progesterone receptor from the
endometrial epithelia on day 10 of the oestrous cycle and
pregnancy has been well documented, the factors involved
with cell specific down-regulation of progesterone receptor
are yet to be established. We propose that several
progesterone stimulated factors activate nuclear factor
kappa B (NF-kB) within the uterine epithelia, which leads to
inhibition of progesterone receptor and concomitant
stimulation of endometrial genes expressed during early
conceptus development. Although oestrogens secreted by pig
conceptuses function to establish pregnancy, timing of
endometrial exposure to oestrogen is critical. Early
oestrogen administration alters the pattern of gene
expression through the NF-kB system desynchronising the
uterine environment for conceptus implantation resulting in
later embryonic loss.},
Language = {eng},
Key = {fds174254}
}
@article{fds174131,
Author = {SL Woo and GA Johnson and BA Smith},
Title = {Mathematical modeling of ligaments and tendons.},
Journal = {Journal of biomechanical engineering},
Volume = {115},
Number = {4B},
Pages = {468-73},
Year = {1993},
Month = {November},
ISSN = {0148-0731},
Keywords = {Animals • Biomechanics • Elasticity •
Ligaments • Models, Biological* • Models,
Structural • Reference Values • Stress,
Physiological • Tendons • Viscosity •
physiology* • physiopathology},
Abstract = {Ligaments and tendons serve a variety of important functions
in maintaining the structure of the human body. Although
abundant literature exists describing experimental
investigations of these tissues, mathematical modeling of
ligaments and tendons also contributes significantly to
understanding their behavior. This paper presents a survey
of developments in mathematical modeling of ligaments and
tendons over the past 20 years. Mathematical descriptions of
ligaments and tendons are identified as either elastic or
viscoelastic, and are discussed in chronological order.
Elastic models assume that ligaments and tendons do not
display time dependent behavior and thus, they focus on
describing the nonlinear aspects of their mechanical
response. On the other hand, viscoelastic models incorporate
time dependent effects into their mathematical description.
In particular, two viscoelastic models are discussed in
detail; quasi-linear viscoelasticity (QLV), which has been
widely used in the past 20 years, and the recently proposed
single integral finite strain (SIFS) model.},
Language = {eng},
Key = {fds174131}
}
@booklet{Woo93,
Author = {S. L. Y. Woo and G. A. Johnson and B. A.
Smith},
Title = {Mathematical-modeling of ligaments and tendons},
Journal = {Journal Of Biomechanical Engineering-transactions Of The
Asme},
Volume = {115},
Number = {4},
Pages = {468 -- 473},
Year = {1993},
Month = {November},
Key = {Woo93}
}
@booklet{Maki91a,
Author = {Maki, JH and Benveniste, H and MacFall, JR and Johnson,
GA},
Title = {Maximization of contrast-to-noise ratio to distinguish
diffusion and microcirculatory flow.},
Journal = {J Magn Reson Imaging},
Volume = {1},
Number = {1},
Pages = {39-46},
Year = {1991},
ISSN = {1053-1807},
url = {http://www.ncbi.nlm.nih.gov/pubmed/1802129},
Abstract = {Optimization of the contrast-to-noise ratio (CNR) is
described for microcirculation magnetic resonance (MR)
imaging techniques based on flow-compensated/flow-dephased
sequences, both with and without even-echo rephasing. The
authors present the most advantageous manner of applying
flow-dephased gradients, such that dephasing is maximal
while diffusion losses are minimal. The theoretical
considerations include phase, diffusion, echo time, and
bandwidth in the determination of the optimal parameters for
microcirculation imaging. Studies in phantoms consisting of
stationary and flowing copper sulfate in Sephadex columns
demonstrate the validity of the calculations. Optimized in
vivo images of a rat stroke model demonstrate the potential
of the flow-compensated/flow-dephased technique and the
importance of optimizing CNR.},
Doi = {10.1002/jmri.1880010105},
Key = {Maki91a}
}
@article{fds132880,
Author = {JH Maki and H Benveniste, JR MacFall and GA Johnson},
Title = {Maximization of contrast-to-noise ratio to distinguish
diffusion and microcirculatory flow.},
Journal = {Journal of magnetic resonance imaging : JMRI, UNITED
STATES},
Volume = {1},
Number = {1},
Pages = {39-46},
ISSN = {1053-1807},
Keywords = {Animals • Cerebral Infarction • Diffusion* •
Magnetic Resonance Imaging • Microcirculation* •
Models, Structural • Rats • diagnosis* •
methods* • physiopathology},
Abstract = {Optimization of the contrast-to-noise ratio (CNR) is
described for microcirculation magnetic resonance (MR)
imaging techniques based on flow-compensated/flow-dephased
sequences, both with and without even-echo rephasing. The
authors present the most advantageous manner of applying
flow-dephased gradients, such that dephasing is maximal
while diffusion losses are minimal. The theoretical
considerations include phase, diffusion, echo time, and
bandwidth in the determination of the optimal parameters for
microcirculation imaging. Studies in phantoms consisting of
stationary and flowing copper sulfate in Sephadex columns
demonstrate the validity of the calculations. Optimized in
vivo images of a rat stroke model demonstrate the potential
of the flow-compensated/flow-dephased technique and the
importance of optimizing CNR.},
Key = {fds132880}
}
@article{fds132831,
Author = {TH Farmer and GP Cofer and GA Johnson},
Title = {Maximizing contrast to noise with inductively coupled
implanted coils.},
Journal = {Investigative radiology, UNITED STATES},
Volume = {25},
Number = {5},
Pages = {552-8},
Year = {1990},
Month = {May},
ISSN = {0020-9996},
Keywords = {Animals • Kidney • Magnetic Resonance Imaging
• Microscopy • Models, Structural • Rats
• Rats, Inbred Strains • anatomy & histology
• instrumentation • instrumentation* •
methods},
Abstract = {Magnetic resonance (MR) microscopy with inductively coupled
implanted coils has been used previously to follow loss and
return of intra-medullary contrast as a result of
nephrotoxic acute tubular necrosis with 117 microns
resolution over a 2000 microns thick slice. The purpose of
the current study was to further investigate the
capabilities of in vivo MR microscopy by combining the
implanted coil imaging technique with spin echo pulse
sequence optimization done through signal-to-noise ratio
(SNR) and contrast-to-noise ratio (CNR) modeling. These
models included consideration of the effects of T2* and
sampling time on signal-to-noise and contrast-to-noise
ratios. They were initially tested with GdCl3 and agar gel
phantoms constructed to the relaxation time and spin density
specifications of the intra-medullary junction which bridges
the outer and inner stripe of the outer medulla. In vivo
microscopy was performed using single turn radiofrequency
(RF) coils that were surgically implanted around the left
kidney of two rats and inductively coupled to an external
"birdcage" body coil. The models revealed maximum CNR per
unit imaging time at a TR of 800 msec. A TE of 16 msec
proved to be the best compromise between loss of transverse
magnetization and decreased bandwidth. These CNR predictions
were supported by the gel phantom and in vivo data.
Maximizing the CNR in the current study enabled us to
improve the resolution of in vivo MR microscopy to 78
microns over a 1000 microns slice with an SNR of 40 and a
CNR of eight in a total imaging time of 54
minutes.},
Key = {fds132831}
}
@booklet{Farmer90,
Author = {Farmer, TH and Cofer, GP and Johnson, GA},
Title = {Maximizing contrast to noise with inductively coupled
implanted coils.},
Journal = {Invest Radiol},
Volume = {25},
Number = {5},
Pages = {552-558},
Year = {1990},
Month = {May},
ISSN = {0020-9996},
url = {http://www.ncbi.nlm.nih.gov/pubmed/2345087},
Abstract = {Magnetic resonance (MR) microscopy with inductively coupled
implanted coils has been used previously to follow loss and
return of intra-medullary contrast as a result of
nephrotoxic acute tubular necrosis with 117 microns
resolution over a 2000 microns thick slice. The purpose of
the current study was to further investigate the
capabilities of in vivo MR microscopy by combining the
implanted coil imaging technique with spin echo pulse
sequence optimization done through signal-to-noise ratio
(SNR) and contrast-to-noise ratio (CNR) modeling. These
models included consideration of the effects of T2* and
sampling time on signal-to-noise and contrast-to-noise
ratios. They were initially tested with GdCl3 and agar gel
phantoms constructed to the relaxation time and spin density
specifications of the intra-medullary junction which bridges
the outer and inner stripe of the outer medulla. In vivo
microscopy was performed using single turn radiofrequency
(RF) coils that were surgically implanted around the left
kidney of two rats and inductively coupled to an external
"birdcage" body coil. The models revealed maximum CNR per
unit imaging time at a TR of 800 msec. A TE of 16 msec
proved to be the best compromise between loss of transverse
magnetization and decreased bandwidth. These CNR predictions
were supported by the gel phantom and in vivo data.
Maximizing the CNR in the current study enabled us to
improve the resolution of in vivo MR microscopy to 78
microns over a 1000 microns slice with an SNR of 40 and a
CNR of eight in a total imaging time of 54
minutes.},
Doi = {10.1097/00004424-199005000-00013},
Key = {Farmer90}
}
@article{fds268781,
Author = {Wetzel, AW and Badea, CT and Pomerantz, SM and Mistry, N and Nave, D and Johnson, GA},
Title = {Measurement and modeling of 4D live mouse heart volumes from
CT time series},
Journal = {Proceedings of SPIE - The International Society for Optical
Engineering},
Volume = {6491},
Publisher = {SPIE},
Year = {2007},
Month = {January},
ISBN = {9780819466044},
ISSN = {0277-786X},
url = {http://dx.doi.org/10.1117/12.704792},
Abstract = {In vivo quantitative studies of cardiac function in mouse
models provide information about cardiac pathophysiology in
more detail than can be obtained in humans. Quantitative
measurements of left ventricular (LV) volume at multiple
contractile phases are particularly important. However, the
mouse heart's small size and rapid motion present challenges
for precise measurement in live animals. Researchers at Duke
University's Center for In Vivo Microscopy (CIVM) have
developed noninvasive time-gated microcomputed tomography
(micro-CT) techniques providing the temporal and spatial
resolutions required for in vivo characterization of cardiac
structure and function. This paper describes analysis of the
resulting reconstructions to produce volume measurements and
corresponding models of heart motion. We believe these are
the most precise noninvasive estimates of in vivo LV volume
currently available. Our technique uses binary mixture
models to directly recover volume estimates from
reconstructed datasets. Unlike methods using segmentation
followed by voxel counting, this approach provides
statistical error estimates and maintains good precision at
high noise levels. This is essential for long term multiple
session experiments that must simultaneously minimize
contrast agent and x-ray doses. The analysis tools are built
into the Pittsburgh Supercomputing Center's Volume Browser
(PSC-VB) that provides networked multi-site data sharing and
collaboration including analysis and visualization
functions. © 2007 SPIE-IS&T.},
Doi = {10.1117/12.704792},
Key = {fds268781}
}
@article{fds132902,
Author = {X Zhang and M Tengowski and L Fasulo and S Botts and SA Suddarth and GA
Johnson},
Title = {Measurement of fat/water ratios in rat liver using 3D
three-point dixon MRI.},
Journal = {Magnetic resonance in medicine : official journal of the
Society of Magnetic Resonance in Medicine / Society of
Magnetic Resonance in Medicine, United States},
Volume = {51},
Number = {4},
Pages = {697-702},
Year = {2004},
Month = {April},
ISSN = {0740-3194},
Keywords = {Adipose Tissue • Animals • Biological Markers
• Body Water • Carrier Proteins •
Dose-Response Relationship, Drug • Drug Evaluation,
Preclinical • Fatty Liver • Female • Humans
• Image Enhancement • Imaging, Three-Dimensional
• Liver • Magnetic Resonance Imaging •
Microsomes, Liver • Rats • Rats, Inbred Strains
• analysis • antagonists & inhibitors •
chemically induced • chemistry* • diagnosis*
• drug effects • methods • methods* •
pathology • pathology*},
Abstract = {Hepatic steatosis, or fatty liver, is commonly observed
during the animal phase of drug safety studies. A
noninvasive three-dimensional (3D) three-point Dixon method
was used to quantitatively evaluate the fatty livers of rats
induced by an experimental microsomal transfer protein (MTP)
inhibitor, in an effort to develop a safety biomarker that
could be translated to human studies. The method was
implemented at 2.0 T for in vivo studies, and at 7.1 T for
higher-resolution magnetic resonance (MR) histologic
studies. In three separate protocols to study dose response
and longitudinal evolution, intrahepatic fatty accumulation
was detected by this method and confirmed by chemical and
histologic assessments. Consistent with the pathologic
changes, the fat/water ratios estimated by the MR technique
increased significantly at doses of 1 mg/kg and 100 mg/kg of
MTP inhibitor after 14 days of continuous administration.
Among the more important findings were: 1). with the 3D
three-point Dixon method, in vivo longitudinal studies of
liver fat distribution can be conducted at significantly
higher resolution than has previously been reported; 2). MR
histology allows delineation of distribution at the
microscopic scale of 0.0024 mm(3) resolution; and 3). the 3D
three-point Dixon technique provides relative estimates of
liver fat content and distribution at a high confidence
level. This technique will be applicable in future studies
in which fatty liver is a potential safety
issue.},
Key = {fds132902}
}
@booklet{Zhang04,
Author = {Zhang, X and Tengowski, M and Fasulo, L and Botts, S and Suddarth, SA and Johnson, GA},
Title = {Measurement of fat/water ratios in rat liver using 3D
three-point dixon MRI.},
Journal = {Magn Reson Med},
Volume = {51},
Number = {4},
Pages = {697-702},
Year = {2004},
Month = {April},
ISSN = {0740-3194},
url = {http://www.ncbi.nlm.nih.gov/pubmed/15065241},
Abstract = {Hepatic steatosis, or fatty liver, is commonly observed
during the animal phase of drug safety studies. A
noninvasive three-dimensional (3D) three-point Dixon method
was used to quantitatively evaluate the fatty livers of rats
induced by an experimental microsomal transfer protein (MTP)
inhibitor, in an effort to develop a safety biomarker that
could be translated to human studies. The method was
implemented at 2.0 T for in vivo studies, and at 7.1 T for
higher-resolution magnetic resonance (MR) histologic
studies. In three separate protocols to study dose response
and longitudinal evolution, intrahepatic fatty accumulation
was detected by this method and confirmed by chemical and
histologic assessments. Consistent with the pathologic
changes, the fat/water ratios estimated by the MR technique
increased significantly at doses of 1 mg/kg and 100 mg/kg of
MTP inhibitor after 14 days of continuous administration.
Among the more important findings were: 1). with the 3D
three-point Dixon method, in vivo longitudinal studies of
liver fat distribution can be conducted at significantly
higher resolution than has previously been reported; 2). MR
histology allows delineation of distribution at the
microscopic scale of 0.0024 mm(3) resolution; and 3). the 3D
three-point Dixon technique provides relative estimates of
liver fat content and distribution at a high confidence
level. This technique will be applicable in future studies
in which fatty liver is a potential safety
issue.},
Doi = {10.1002/mrm.20005},
Key = {Zhang04}
}
@article{fds132882,
Author = {BT Chen and AC Brau and GA Johnson},
Title = {Measurement of regional lung function in rats using
hyperpolarized 3helium dynamic MRI.},
Journal = {Magnetic resonance in medicine : official journal of the
Society of Magnetic Resonance in Medicine / Society of
Magnetic Resonance in Medicine, United States},
Volume = {49},
Number = {1},
Pages = {78-88},
Year = {2003},
Month = {January},
ISSN = {0740-3194},
Keywords = {Animals • Female • Helium • Image Processing,
Computer-Assisted • Isotopes • Lung •
Magnetic Resonance Imaging, Cine* • Phantoms, Imaging
• Rats • Rats, Sprague-Dawley • Respiratory
Mechanics* • Tidal Volume • anatomy & histology
• diagnostic use • physiology*},
Abstract = {Dynamic regional lung function was investigated in rats
using a radial acquisition cine (RA-CINE) pulse sequence
together with hyperpolarized (HP) (3)He gas delivered by a
constant flow ventilator. Based on regional differences in
the behavior of inspired air, the lung was conceptually
divided into two regions (the major airways and the
peripheral airspace) for purposes of functional analysis. To
measure regional function in the major airways, a large RF
flip angle (24 degrees) was applied to reduce (3)He
magnetization in the peripheral airspace, and signal
intensity (SI) was normalized with the projected airway
diameter to estimate local airflow. Higher normalized signal
intensity was observed in the left branch airway as compared
to the right branch airway. To determine regional function
in the peripheral airspace, a small RF flip angle (6
degrees) was used. Incremental increases of peripheral SI in
successive lung images were consistent with the increase in
lung volume. A new "skipping" scanning strategy using dummy
frames allows a trade-off between the number of frames
acquired for dynamic information, the RF flip angle, and the
penetration depth of (3)He magnetization into the lung. This
work provides a novel approach to simultaneously assess
dynamic regional function and morphology.},
Key = {fds132882}
}
@booklet{Chen03,
Author = {Chen, BT and Brau, ACS and Johnson, GA},
Title = {Measurement of regional lung function in rats using
hyperpolarized 3helium dynamic MRI.},
Journal = {Magn Reson Med},
Volume = {49},
Number = {1},
Pages = {78-88},
Year = {2003},
Month = {January},
ISSN = {0740-3194},
url = {http://www.ncbi.nlm.nih.gov/pubmed/12509822},
Abstract = {Dynamic regional lung function was investigated in rats
using a radial acquisition cine (RA-CINE) pulse sequence
together with hyperpolarized (HP) (3)He gas delivered by a
constant flow ventilator. Based on regional differences in
the behavior of inspired air, the lung was conceptually
divided into two regions (the major airways and the
peripheral airspace) for purposes of functional analysis. To
measure regional function in the major airways, a large RF
flip angle (24 degrees) was applied to reduce (3)He
magnetization in the peripheral airspace, and signal
intensity (SI) was normalized with the projected airway
diameter to estimate local airflow. Higher normalized signal
intensity was observed in the left branch airway as compared
to the right branch airway. To determine regional function
in the peripheral airspace, a small RF flip angle (6
degrees) was used. Incremental increases of peripheral SI in
successive lung images were consistent with the increase in
lung volume. A new "skipping" scanning strategy using dummy
frames allows a trade-off between the number of frames
acquired for dynamic information, the RF flip angle, and the
penetration depth of (3)He magnetization into the lung. This
work provides a novel approach to simultaneously assess
dynamic regional function and morphology.},
Doi = {10.1002/mrm.10336},
Key = {Chen03}
}
@article{fds268938,
Author = {Möller, HE and Hedlund, LW and Chen, XJ and Carey, MR and Chawla, MS and Wheeler, CT and Johnson, GA},
Title = {Measurements of hyperpolarized gas properties in the lung.
Part III: (3)He T(1).},
Journal = {Magn Reson Med},
Volume = {45},
Number = {3},
Pages = {421-430},
Year = {2001},
Month = {March},
ISSN = {0740-3194},
url = {http://www.ncbi.nlm.nih.gov/pubmed/11241699},
Keywords = {Animals • Cardiac Output • Functional Residual
Capacity • Guinea Pigs • Heart Arrest, Induced
• Helium* • Image Enhancement* • Isotopes
• Lung • Magnetic Resonance Spectroscopy* •
Oxygen • Pulmonary Gas Exchange • blood •
pathology • physiology • physiology*},
Abstract = {Hyperpolarized (3)He spin-lattice relaxation was
investigated in the guinea pig lung using spectroscopy and
imaging techniques with a repetitive RF pulse series. T(1)
was dominated by interactions with oxygen and was used to
measure the alveolar O(2) partial pressure. In animals
ventilated with a mixture of 79% (3)He and 21% O(2), T(1)
dropped from 19.6 sec in vivo to 14.6 sec after cardiac
arrest, reflecting the termination of the intrapulmonary gas
exchange. The initial difference in oxygen concentration
between inspired and alveolar air, and the temporal decay
during apnea were related to functional parameters.
Estimates of oxygen uptake were 29 +/- 11 mL min(-1) kg(-1)
under normoxic conditions, and 9.0 +/- 2.0 mL min(-1) kg(-1)
under hypoxic conditions. Cardiac output was estimated to be
400 +/- 160 mL min(-1) kg(-1). The functional residual
capacity derived from spirometric magnetic resonance
experiments varied with body mass between 5.4 +/- 0.3 mL and
10.7 +/- 1.1 mL. Magn Reson Med 45:421-430,
2001.},
Doi = {10.1002/1522-2594(200103)45:3<421::aid-mrm1055>3.0.co;2-k},
Key = {fds268938}
}
@booklet{Moller01,
Author = {H. E. Moller and L. W. Hedlund and X. J. Chen and M. R.
Carey and M. S. Chawla and C. T. Wheeler and G. A.
Johnson},
Title = {Measurements of hyperpolarized gas properties in the lung.
Part III: He-3 T-1},
Journal = {Magnetic Resonance In Medicine},
Volume = {45},
Number = {3},
Pages = {421 -- 430},
Year = {2001},
Month = {March},
Key = {Moller01}
}
@article{fds367385,
Author = {Nicholls, PJ and Pack, TF and Urs, NM and Kumar, S and Zhou, Y and Ichim,
G and Ginzel, JD and Turu, G and Calabrese, E and Roberts, WL and Fan, P and Ostapchenko, VG and Guzman Lenis and MS and Beraldo, F and Hatina, J and Prado, VF and Prado, MAM and Spasojevic, I and Snyder, JC and Dzirasa,
K and Johnson, GA and Caron, MG},
Title = {Measuring Nonapoptotic Caspase Activity with a Transgenic
Reporter in Mice.},
Journal = {eNeuro},
Volume = {9},
Number = {5},
Year = {2022},
url = {http://dx.doi.org/10.1523/ENEURO.0147-21.2022},
Abstract = {The protease caspase-3 is a key mediator of apoptotic
programmed cell death. But weak or transient caspase
activity can contribute to neuronal differentiation, axonal
pathfinding, and synaptic long-term depression. Despite the
importance of sublethal, or nonapoptotic, caspase activity
in neurodevelopment and neural plasticity, there has been no
simple method for mapping and quantifying nonapoptotic
caspase activity (NACA) in rodent brains. We therefore
generated a transgenic mouse expressing a highly sensitive
and specific fluorescent reporter of caspase activity, with
peak signal localized to the nucleus. As a proof of concept,
we first obtained evidence that NACA influences
neurophysiology in an amygdalar circuit. Then focusing on
the amygdala, we were able to quantify a sex-specific
persistent elevation in caspase activity in females after
restraint stress. This simple in vivo caspase activity
reporter will facilitate systems-level studies of apoptotic
and nonapoptotic phenomena in behavioral and pathologic
models.},
Doi = {10.1523/ENEURO.0147-21.2022},
Key = {fds367385}
}
@booklet{Qiu98,
Author = {H. H. Qiu and L. W. Hedlund and M. R. Neuman and C. R.
Edwards and R. D. Black and G. P. Cofer and G. A.
Johnson},
Title = {Measuring the progression of foreign-body reaction to
silicone implants using in vivo MR microscopy},
Journal = {Ieee Transactions On Biomedical Engineering},
Volume = {45},
Number = {7},
Pages = {921 -- 927},
Year = {1998},
Month = {July},
Key = {Qiu98}
}
@article{fds268995,
Author = {Qiu, HH and Hedlund, LW and Neuman, MR and Edwards, CR and Black, RD and Cofer, GP and Johnson, GA},
Title = {Measuring the progression of foreign-body reaction to
silicone implants using in vivo MR microscopy.},
Journal = {IEEE Trans Biomed Eng},
Volume = {45},
Number = {7},
Pages = {921-927},
Year = {1998},
Month = {July},
ISSN = {0018-9294},
url = {http://www.ncbi.nlm.nih.gov/pubmed/9644901},
Keywords = {Animals • Female • Foreign-Body Reaction •
Magnetic Resonance Imaging* • Microscopy • Neck
• Prostheses and Implants • Rats • Rats,
Sprague-Dawley • Silicones • adverse effects*
• etiology • methods • pathology* •
surgery},
Abstract = {We used in vivo magnetic resonance (MR) microscopy to follow
the growth of fibrous capsule as a foreign body reaction to
silicone implants in rats. Anesthetized rats were imaged 1,
7, 14, and 28 days after silicone-coated MR imaging coils
were sutured to their neck muscles. On the twenty-eighth
day, rats were sacrificed and coils and adjacent tissues
were removed en bloc and fixed in formalin, reimaged with
MR, and sectioned for conventional histology.
Three-dimensional (3-D) spin-echo [3DFT] acquisition gave
in-plane resolution of 32 x 32 microns in vivo and 16 x 16
microns ex vivo. All MR images showed a diffuse band of
elevated signal intensity between the silicone of the coil
and adjacent tissue. The border of the hyperintense band was
thin and not well defined at seven days post-implantation.
From 7-28 days, the band showed relatively homogeneous
signal intensity and its thickness increased 44% on the
rectus muscle side and 78% on the subcutaneous side. The
capsule thickness determined either by MR in vivo and ex
vivo microscopy or conventional histology was not
significantly different, and there was a significant
correlation between thickness measurements among those
methods. MR in vivo microscopy provides sufficient
resolution and spatial information to serially evaluate the
growth of the foreign body fibrous capsule over time, thus
achieving greater accuracy and consistency in
measurements.},
Doi = {10.1109/10.686800},
Key = {fds268995}
}
@article{fds268887,
Author = {Hedlund, LW and Johnson, GA},
Title = {Mechanical ventilation for imaging the small animal
lung.},
Journal = {ILAR J},
Volume = {43},
Number = {3},
Pages = {159-174},
Year = {2002},
ISSN = {1084-2020},
url = {http://www.ncbi.nlm.nih.gov/pubmed/12105383},
Keywords = {Anatomy, Cross-Sectional • Animals • Animals,
Laboratory • Disease Models, Animal • Guinea Pigs
• Lung* • Magnetic Resonance Imaging • Rats
• Respiration, Artificial • anatomy & histology
• instrumentation • methods* • physiology
• physiology* • physiopathology},
Abstract = {This review emphasizes some of the challenges and benefits
of in vivo imaging of the small animal lung. Because
mechanical ventilation plays a key role in high-quality,
high-resolution imaging of the small animal lung, the
article focuses particularly on the problems of ventilation
support, control of breathing motion and lung volume, and
imaging during different phases of the breathing cycle.
Solutions for these problems are discussed primarily in
relation to magnetic resonance imaging, both conventional
proton imaging and the newer, hyperpolarized helium imaging
of pulmonary airways. Examples of applications of these
imaging solutions to normal and diseased lung are
illustrated in the rat and guinea pig. Although difficult to
perform, pulmonary imaging in the small animal can be a
valuable source of information not only for the normal lung,
but also for the lung challenged by disease.},
Doi = {10.1093/ilar.43.3.159},
Key = {fds268887}
}
@booklet{Hansen99,
Author = {T. R. Hansen and K. J. Austin and D. J. Perry and J. K. Pru and M. G. Teixeira and G. A. Johnson},
Title = {Mechanism of action of interferon-tau in the uterus during
early pregnancy},
Journal = {Journal Of Reproduction And Fertility},
Pages = {329 -- 339},
Year = {1999},
Key = {Hansen99}
}
@article{fds174074,
Author = {TR Hansen and KJ Austin and DJ Perry and JK Pru and MG Teixeira and GA
Johnson},
Title = {Mechanism of action of interferon-tau in the uterus during
early pregnancy.},
Journal = {Journal of reproduction and fertility. Supplement},
Volume = {54},
Pages = {329-39},
Year = {1999},
ISSN = {0449-3087},
Keywords = {Amino Acid Sequence • Animals • Cattle •
Chemokine CXCL2 • Chemokine CXCL6 • Chemokines
• Chemokines, CXC • Corpus Luteum Maintenance
• Dinoprost • Dinoprostone • Embryo,
Mammalian • Female • Interferon Type I •
Molecular Sequence Data • Pregnancy • Pregnancy
Proteins • Pregnancy, Animal • Progesterone •
Protein Binding • Receptors, Estradiol •
Receptors, Interferon • Ubiquitins • genetics
• metabolism • metabolism* •
physiology*},
Abstract = {Early pregnancy is maintained in ruminants through the
actions of conceptus-derived interferon (IFN)-tau on the
endometrium. IFN-tau alters uterine release of PGF2 alpha'
which results in rescue of the corpus luteum and continued
release of progesterone. The mechanism of action of IFN-tau
includes inhibition of oestradiol receptors, consequent
reduction in oxytocin receptors, activation of a
cyclooxygenase inhibitor, and a shift in the PGs to favour
PGE2 over PGF2 alpha' IFN-tau also induces several
endometrial proteins that may be critical for survival of
the developing embryo. One endometrial protein induced by
pregnancy and IFN-tau has been identified as bovine
granulocyte chemotactic protein-2 (bGCP-2). This chemotactic
cytokine (chemokine) has been used as a marker to delineate
IFN-tau from IFN-alpha responses in the endometrium. A
second protein, called ubiquitin cross-reactive protein
(UCRP), resembles a tandem ubiquitin repeat. UCRP becomes
conjugated to cytosolic endometrial proteins in response to
IFN-tau and pregnancy. Proteins conjugated to UCRP are
either modulated or targeted for processing through the
proteasome. The action of IFN-tau is mediated by induction
of signal transducer and activator of transcription 1
(STAT-1), STAT-2 and interferon regulatory factor 1 (IRF-1)
transcription factors. Induction of these transcription
factors, the alpha chemokines and UCRP is the prelude to
maternal recognition of pregnancy in ruminants.},
Language = {eng},
Key = {fds174074}
}
@booklet{Mesfin87,
Author = {G. M. Mesfin and G. A. Johnson and M. J. Higgins and D. F.
Morris},
Title = {Mechanism of anestrus in rats treated with an
antihypertensive agent, losulazine hydrochloride},
Journal = {Toxicology And Applied Pharmacology},
Volume = {87},
Number = {1},
Pages = {91 -- 101},
Year = {1987},
Month = {January},
Key = {Mesfin87}
}
@article{fds174220,
Author = {GM Mesfin and GA Johnson and MJ Higgins and DF Morris},
Title = {Mechanism of anestrus in rats treated with an
antihypertensive agent, losulazine hydrochloride.},
Journal = {Toxicology and applied pharmacology},
Volume = {87},
Number = {1},
Pages = {91-101},
Year = {1987},
Month = {January},
ISSN = {0041-008X},
Keywords = {Anestrus • Animals • Antihypertensive Agents
• Body Weight • Bromocriptine •
Catecholamines • Dopamine • Estrus • Female
• Follicle Stimulating Hormone • Genitalia, Female
• Hypothalamus • Piperazines • Progesterone
• Prolactin • Rats • Rats, Inbred Strains
• blood • drug effects • drug effects* •
metabolism • pharmacology • pharmacology*},
Abstract = {The mechanism of anestrus in rats treated with losulazine, a
peripheral sympatholytic antihypertensive agent, was
investigated by determining its effect on hypothalamic
catecholamines and serum sex hormones and by evaluating the
influence of bromocriptine on the reproductive functions of
rats treated with losulazine. Groups of six female Upjohn
Sprague-Dawley rats were treated orally with 10 mg/kg/day of
losulazine and/or 18.75 mg/kg/day of bromocriptine for 15 or
27 days. Six rats were treated with losulazine plus 6.25
mg/kg/day of bromocriptine for 16 days followed by
losulazine alone for 11 days. Rats treated with losulazine
only were depleted of hypothalamic catecholamines, were
hyperprolactinemic, and had interrupted estrous cycles and
attenuated vaginal mucosa. Treatment with bromocriptine, a
dopamine receptor agonist, resulted in suppression of serum
prolactin and normal estrous cycles. Rats reverted back to
hyperprolactinemia and anestrus shortly after bromocriptine
withdrawal. These results suggest that hyperprolactinemia
mediated through hypothalamic dopamine depletion is the
mechanism of anestrus in rats treated with
losulazine.},
Language = {eng},
Key = {fds174220}
}
@article{fds132804,
Author = {H Benveniste and LW Hedlund and GA Johnson},
Title = {Mechanism of detection of acute cerebral ischemia in rats by
diffusion-weighted magnetic resonance microscopy.},
Journal = {Stroke; a journal of cerebral circulation, UNITED
STATES},
Volume = {23},
Number = {5},
Pages = {746-54},
Year = {1992},
Month = {May},
ISSN = {0039-2499},
Keywords = {Acute Disease • Animals • Brain • Brain
Ischemia • Diffusion • Female • Glutamates
• Glutamic Acid • Magnetic Resonance Imaging
• N-Methylaspartate • Ouabain • Rats •
Rats, Inbred Strains • Staining and Labeling •
diagnosis* • drug effects • metabolism •
methods* • pharmacology},
Abstract = {BACKGROUND AND PURPOSE: The aim of this study was to measure
apparent diffusion coefficients in rat brain tissue exposed
to ouabain, glutamate, and N-methyl-D-aspartate and to
compare them with apparent diffusion coefficients found in
acute cerebral ischemia. METHODS: The apparent diffusion
coefficient was measured using magnetic resonance microscopy
in four groups of Sprague-Dawley rats after occlusion of the
right middle cerebral artery and ipsilateral common carotid
artery (n = 7), after ouabain exposure (n = 6), during
glutamate exposure (n = 7), or during N-methyl-D-aspartate
exposure (n = 3). Ouabain, glutamate, and
N-methyl-D-aspartate were applied via an intracerebrally
implanted microdialysis membrane. RESULTS: Three hours after
the induction of focal cerebral ischemia, a 33% reduction in
the apparent diffusion coefficient was observed in the right
dorsolateral corpus striatum and olfactory cortex. After
ouabain exposure, reductions in the apparent diffusion
coefficient were observed within a 1,500-microns radius of
the microdialysis membrane. Quantitative analysis revealed
that apparent diffusion coefficient values in ischemic and
ouabain-exposed tissue fell within the same range. Glutamate
and N-methyl-D-aspartate reduced the brain tissue apparent
diffusion coefficient by 35% and 40%, respectively.
CONCLUSIONS: On the basis of these findings, we conclude
that ischemia-induced apparent diffusion coefficient
reductions are likely caused by a shift of extracellular to
intracellular water.},
Key = {fds132804}
}
@booklet{Benveniste92,
Author = {Benveniste, H and Hedlund, LW and Johnson, GA},
Title = {Mechanism of detection of acute cerebral ischemia in rats by
diffusion-weighted magnetic resonance microscopy.},
Journal = {Stroke},
Volume = {23},
Number = {5},
Pages = {746-754},
Year = {1992},
Month = {May},
ISSN = {0039-2499},
url = {http://www.ncbi.nlm.nih.gov/pubmed/1374575},
Abstract = {BACKGROUND AND PURPOSE: The aim of this study was to measure
apparent diffusion coefficients in rat brain tissue exposed
to ouabain, glutamate, and N-methyl-D-aspartate and to
compare them with apparent diffusion coefficients found in
acute cerebral ischemia. METHODS: The apparent diffusion
coefficient was measured using magnetic resonance microscopy
in four groups of Sprague-Dawley rats after occlusion of the
right middle cerebral artery and ipsilateral common carotid
artery (n = 7), after ouabain exposure (n = 6), during
glutamate exposure (n = 7), or during N-methyl-D-aspartate
exposure (n = 3). Ouabain, glutamate, and
N-methyl-D-aspartate were applied via an intracerebrally
implanted microdialysis membrane. RESULTS: Three hours after
the induction of focal cerebral ischemia, a 33% reduction in
the apparent diffusion coefficient was observed in the right
dorsolateral corpus striatum and olfactory cortex. After
ouabain exposure, reductions in the apparent diffusion
coefficient were observed within a 1,500-microns radius of
the microdialysis membrane. Quantitative analysis revealed
that apparent diffusion coefficient values in ischemic and
ouabain-exposed tissue fell within the same range. Glutamate
and N-methyl-D-aspartate reduced the brain tissue apparent
diffusion coefficient by 35% and 40%, respectively.
CONCLUSIONS: On the basis of these findings, we conclude
that ischemia-induced apparent diffusion coefficient
reductions are likely caused by a shift of extracellular to
intracellular water.},
Doi = {10.1161/01.str.23.5.746},
Key = {Benveniste92}
}
@article{fds174270,
Author = {GA Johnson and EG Kim and SJ Boukma},
Title = {Mechanism of norepinephrine depletion by
5-hydroxytryptophan.},
Journal = {Proceedings of the Society for Experimental Biology and
Medicine. Society for Experimental Biology and Medicine (New
York, N.Y.)},
Volume = {128},
Number = {2},
Pages = {509-12},
Year = {1968},
Month = {June},
ISSN = {0037-9727},
Keywords = {5-Hydroxytryptophan • Animals • Brain • Brain
Chemistry • Carbon Isotopes • Injections,
Intraperitoneal • Injections, Intravenous • Male
• Methyltyrosines • Norepinephrine • Rats
• Tyrosine • antagonists & inhibitors* •
biosynthesis • metabolism • metabolism* •
pharmacology • pharmacology*},
Language = {eng},
Key = {fds174270}
}
@article{fds204254,
Author = {FW Bazer and G Song and J Kim and DW Erikson and GA Johnson and RC
Burghardt, H Gao and M Carey Satterfield and TE Spencer and G
Wu},
Title = {Mechanistic mammalian target of rapamycin (MTOR) cell
signaling: Effects of select nutrients and secreted
phosphoprotein 1 on development of mammalian
conceptuses.},
Journal = {Molecular and cellular endocrinology},
Year = {2011},
Month = {September},
ISSN = {1872-8057},
url = {http://dx.doi.org/10.1016/j.mce.2011.08.026},
Abstract = {Morphological differentiation of uterine glands in mammals
is a postnatal event vulnerable to adverse effects of
endocrine disruptors. Exposure of ewe lambs to a progestin
from birth to postnatal day 56 prevents development of
uterine glands and, as adults, the ewes are unable to
exhibit estrous cycles or maintain pregnancy. Uterine
epithelia secrete proteins and transport nutrients into the
uterine lumen necessary for conceptus development, pregnancy
recognition signaling and implantation, including arginine
and secreted phosphoprotein 1 (SPP1). Arginine can be
metabolized to nitric oxide and to polyamines or act
directly to activate MTOR cell signaling to stimulate
proliferation, migration, and mRNA translation in
trophectoderm cells. SPP1 binds αvβ3 and α5β1 integrins
and induces focal adhesion assembly, adhesion and migration
of conceptus trophectoderm cells during implantation. Thus,
arginine and SPP1 mediate growth, migration, cytoskeletal
remodeling and adhesion of trophectoderm essential for
pregnancy recognition signaling and implantation.},
Language = {ENG},
Doi = {10.1016/j.mce.2011.08.026},
Key = {fds204254}
}
@booklet{Orlander02,
Author = {J. D. Orlander and B. G. Fincke and D. Hermanns and G. A.
Johnson},
Title = {Medical residents' first clearly remembered experiences of
giving bad news},
Journal = {Journal Of General Internal Medicine},
Volume = {17},
Number = {11},
Pages = {825 -- 840},
Year = {2002},
Month = {November},
Key = {Orlander02}
}
@article{fds174101,
Author = {JD Orlander and BG Fincke and D Hermanns and GA Johnson},
Title = {Medical residents' first clearly remembered experiences of
giving bad news.},
Journal = {Journal of general internal medicine},
Volume = {17},
Number = {11},
Pages = {825-31},
Year = {2002},
Month = {November},
ISSN = {0884-8734},
Keywords = {Adult • Communication • Female • Health
Surveys • Humans • Internal Medicine* •
Internship and Residency* • Male •
Physician-Patient Relations*},
Abstract = {CONTEXT: Communication of bad news to patients or families
is a difficult task that requires skill and sensitivity.
Little is known about doctors' formative experiences in
giving bad news, what guidance they receive, or what lessons
they learn in the process. OBJECTIVE: To learn the
circumstances in which medical residents first delivered bad
news to patients or families, the nature of their
experience, and their opinions about how best to develop the
needed skills. DESIGN: Confidential mailed survey. SETTING
AND SUBJECTS: All medicine house officers at 2 urban,
university-based residency programs in Boston. MAIN OUTCOME
MEASURES: Details of medical residents' first clearly
remembered experiences of giving bad news to a patient or
family member; year in training; familiarity with the
patient; information about any planning prior to,
observation of, or discussion after their first experience;
and the usefulness of such discussions. We also asked
general questions about delivering bad news, such as how
often this was done, as well as asking for opinions about
actual and desired training. RESULTS: One hundred
twenty-nine of two hundred thirteen surveys (61%) were
returned. Most (73%) trainees first delivered bad news while
a medical student or intern. For this first experience, most
(61%) knew the patient for just hours or days. Only 59%
engaged in any planning for the encounter. An attending
physician was present in 6 (5%) instances, and a more-senior
trainee in 14 (11%) others. Sixty-five percent of subjects
debriefed with at least 1 other person after the encounter,
frequently with a lesser-trained physician or a member of
their own family. Debriefing focused on the reaction of
those who were given the bad news and the reaction of the
trainee. When there were discussions with more-senior
physicians, before or after the encounter, these were judged
to be helpful approximately 80% of the time. Most subjects
had given bad news between 5 and 20 times, yet 10% had never
been observed doing so. Only 81 of 128 (63%) had ever
observed an attending delivering bad news, but those who did
found it helpful 96% of the time. On 7-point scales,
subjects rated the importance of skills in delivering bad
news highly, (mean 6.8), believed such skill can be improved
(mean 6.6), and thought that more guidance should be offered
to them during such activity (mean 5.8). CONCLUSION: Medical
students and residents frequently deliver bad news to
patients and families. This responsibility begins early in
training. In spite of their inexperience, many do not appear
to receive adequate guidance or support during their
earliest formative experiences.},
Language = {eng},
Key = {fds174101}
}
@booklet{Johnson97d,
Author = {G. A. Johnson},
Title = {Medicinal marijuana?},
Journal = {New England Journal Of Medicine},
Volume = {336},
Number = {16},
Pages = {1186 -- 1186},
Year = {1997},
Month = {April},
Key = {Johnson97d}
}
@article{fds370388,
Author = {Johnson, GA and Tian, Y and Ashbrook, DG and Cofer, GP and Cook, JJ and Gee, JC and Hall, A and Hornburg, K and Kaczorowski, CC and Qi, Y and Yeh,
F-C and Wang, N and White, LE and Williams, RW},
Title = {Merged magnetic resonance and light sheet microscopy of the
whole mouse brain.},
Journal = {Proc Natl Acad Sci U S A},
Volume = {120},
Number = {17},
Pages = {e2218617120},
Year = {2023},
Month = {April},
url = {http://dx.doi.org/10.1073/pnas.2218617120},
Abstract = {We have developed workflows to align 3D magnetic resonance
histology (MRH) of the mouse brain with light sheet
microscopy (LSM) and 3D delineations of the same specimen.
We start with MRH of the brain in the skull with gradient
echo and diffusion tensor imaging (DTI) at 15 μm isotropic
resolution which is ~ 1,000 times higher than that of most
preclinical MRI. Connectomes are generated with
superresolution tract density images of ~5 μm. Brains are
cleared, stained for selected proteins, and imaged by LSM at
1.8 μm/pixel. LSM data are registered into the reference
MRH space with labels derived from the ABA common coordinate
framework. The result is a high-dimensional integrated volume with registration
(HiDiver) with alignment precision better than 50 µm.
Throughput is sufficiently high that HiDiver is being used
in quantitative studies of the impact of gene variants and
aging on mouse brain cytoarchitecture and
connectomics.},
Doi = {10.1073/pnas.2218617120},
Key = {fds370388}
}
@article{fds174269,
Author = {GA JOHNSON and CR KISTLER and RH McCLUER},
Title = {Metabolism of exogenous sialic acid in the
rat.},
Journal = {Proceedings of the Society for Experimental Biology and
Medicine. Society for Experimental Biology and Medicine (New
York, N.Y.)},
Volume = {106},
Pages = {124-7},
Year = {1961},
Month = {January},
ISSN = {0037-9727},
Language = {eng},
Key = {fds174269}
}
@booklet{Korobkin81,
Author = {KOROBKIN, M and JOHNSON, GA and BREIMAN, RS},
Title = {METHODOLOGIC CONSIDERATIONS IN MULTIPLANAR REFORMATTING OF
TRANSAXIAL CT IMAGES},
Journal = {Investigative Radiology},
Volume = {16},
Number = {5},
Pages = {374-374},
Publisher = {Ovid Technologies (Wolters Kluwer Health)},
Year = {1981},
Month = {September},
ISSN = {0020-9996},
url = {http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:A1981MK56200027&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=47d3190e77e5a3a53558812f597b0b92},
Doi = {10.1097/00004424-198109000-00027},
Key = {Korobkin81}
}
@booklet{Macfall87,
Author = {MacFall, JR and Wehrli, FW and Breger, RK and Johnson,
GA},
Title = {Methodology for the measurement and analysis of relaxation
times in proton imaging.},
Journal = {Magn Reson Imaging},
Volume = {5},
Number = {3},
Pages = {209-220},
Year = {1987},
ISSN = {0730-725X},
url = {http://www.ncbi.nlm.nih.gov/pubmed/3041152},
Abstract = {Measurements of proton T1 and T2 were performed on GdCl3
solutions (20 less than T2 less than 500 msec, 90 less than
T1 less than 1000 msec) on large-bore NMR imaging systems
operating at 1.0T and 1.5T. CPMG multi-echo (ME), multiple
saturation recovery (MSR) and modified fast inversion
recovery (MFIR) pulse sequences as well as a sequence that
combines and interleaves T1 and T2 weighted data acquisition
(which we call "multiple saturation-recovery multiple-echo"
(MSRME) were used. The relaxation data are compared to those
obtained on a small bore NMR spectrometer operated at 1.5T.
T1 and T2 values for the solutions were found to be the same
within 10% for the two fields. Reproducibility of
measurements of T1, T2 and the unnormalized spin density of
the solutions was better than 5%. Systematic errors,
amenable to correction through calibration, are noted in the
imager T1 and T2 values. T1 and T2 values for some typical
neural tissues at 1.5T and body tissue at 1.0T for human
volunteers were obtained and are tabulated.},
Doi = {10.1016/0730-725x(87)90022-1},
Key = {Macfall87}
}
@article{fds132754,
Author = {JR MacFall and FW Wehrli and RK Breger and GA Johnson},
Title = {Methodology for the measurement and analysis of relaxation
times in proton imaging.},
Journal = {Magnetic resonance imaging, UNITED STATES},
Volume = {5},
Number = {3},
Pages = {209-20},
Year = {1987},
ISSN = {0730-725X},
Keywords = {Gadolinium • Magnetic Resonance Spectroscopy •
Protons • Solutions • instrumentation •
methods*},
Abstract = {Measurements of proton T1 and T2 were performed on GdCl3
solutions (20 less than T2 less than 500 msec, 90 less than
T1 less than 1000 msec) on large-bore NMR imaging systems
operating at 1.0T and 1.5T. CPMG multi-echo (ME), multiple
saturation recovery (MSR) and modified fast inversion
recovery (MFIR) pulse sequences as well as a sequence that
combines and interleaves T1 and T2 weighted data acquisition
(which we call "multiple saturation-recovery multiple-echo"
(MSRME) were used. The relaxation data are compared to those
obtained on a small bore NMR spectrometer operated at 1.5T.
T1 and T2 values for the solutions were found to be the same
within 10% for the two fields. Reproducibility of
measurements of T1, T2 and the unnormalized spin density of
the solutions was better than 5%. Systematic errors,
amenable to correction through calibration, are noted in the
imager T1 and T2 values. T1 and T2 values for some typical
neural tissues at 1.5T and body tissue at 1.0T for human
volunteers were obtained and are tabulated.},
Key = {fds132754}
}
@article{fds174147,
Author = {BM Markaverich and RR Gregory and MA Alejandro and JH Clark and GA
Johnson, BS Middleditch},
Title = {Methyl p-hydroxyphenyllactate. An inhibitor of cell growth
and proliferation and an endogenous ligand for nuclear
type-II binding sites.},
Journal = {The Journal of biological chemistry},
Volume = {263},
Number = {15},
Pages = {7203-10},
Year = {1988},
Month = {May},
ISSN = {0021-9258},
Keywords = {Animals • Breast Neoplasms • Cell Division •
Cell Line • Cell Nucleus • Chromatography, Gel
• Chromatography, High Pressure Liquid • Female
• Humans • Lactates • Mass Spectrometry
• Ovariectomy • Rats • Rats, Inbred Strains
• Receptors, Estradiol • Receptors, Estrogen
• drug effects* • isolation & purification •
metabolism • metabolism* • pharmacology*},
Abstract = {We previously described and partially characterized
endogenous ligands for nuclear type II sites in normal and
malignant tissues. Chromatography of these ligands on
Sephadex LH-20 revealed that two peaks with binding activity
(alpha and beta) could be resolved. The beta-peak component
was present in all normal tissues that we examined, but not
in malignant tissues, and it inhibited the growth of MCF-7
human breast cancer cells in vitro. Conversely, the
alpha-peak component was found to be present in both normal
and malignant tissues, and did not inhibit MCF-7 cell
growth. The present studies describe the purification and
identification of the alpha-peak and beta-peak components in
bovine serum and an assessment of the effects of these
compounds on normal and malignant cell growth. Gas
chromatography-mass spectroscopy analysis of the purified
beta-peak component demonstrated that the compound was
methyl p-hydroxyphenyllactate (MeHPLA). Competition analysis
revealed that MeHPLA binds to nuclear type II sites with a
high binding affinity, while physiological levels of this
compound blocked estradiol stimulation of uterine growth in
vivo and inhibited the growth of MCF-7 human breast cancer
cells in vitro. The alpha-peak component was found to be the
corresponding acid, p-hydroxyphenyllactic acid (HPLA). This
compound interacted with nuclear type II sites with a
relatively low affinity and did not block uterotropic
response to estradiol or inhibit MCF-7 cell growth. These
studies demonstrate that HPLA and MeHPLA are ligands for
nuclear type II sites and that MeHPLA may be a very
important regulator of normal and malignant cell
growth.},
Language = {eng},
Key = {fds174147}
}
@booklet{Markaverich88b,
Author = {B. M. Markaverich and R. R. Gregory and M. A. Alejandro and J. H. Clark and G. A. Johnson and B. S. Middleditch},
Title = {Methyl para-hydroxyphenyllactate - an inhibitor of
cell-growth and proliferation and an endogenous ligand for
nuclear type-ii binding-sites},
Journal = {Journal Of Biological Chemistry},
Volume = {263},
Number = {15},
Pages = {7203 -- 7210},
Year = {1988},
Month = {May},
Key = {Markaverich88b}
}
@booklet{Markaverich88,
Author = {B. M. Markaverich and R. R. Gregory and M. A. Alejandro and G. A. Johnson and B. S. Middleditch},
Title = {Methyl para-hydroxyphenyllactate - identification in
rat-liver extracts},
Journal = {Journal Of High Resolution Chromatography \& Chromatography
Communications},
Volume = {11},
Number = {8},
Pages = {605 -- 607},
Year = {1988},
Month = {August},
Key = {Markaverich88}
}
@article{064610243353,
Author = {De Lin and M and Hedlund, L and Johnson, GA},
Title = {Micro radiography imaging of the rodent with phenylephrine
induced vascular hypertension},
Journal = {2006 3rd IEEE International Symposium on Biomedical Imaging:
From Nano to Macro - Proceedings},
Volume = {2006},
Pages = {610-613},
Address = {Arlington, VA, United States},
Year = {2006},
Month = {November},
ISBN = {9780780395770},
Keywords = {Medical imaging;Blood;Biological organs;Angiography;Data
acquisition;Image analysis;},
Abstract = {Vascular tonicity plays a major role in regulating the blood
pressure and the perfusion of organs, and hypertension is a
major cause of morbidity and mortality in humans. We use
phenylephrine, a vasoconstrictor, to create a model of
hypertension in the rat. This work demonstrates the use of a
micro-X-ray digital subtraction angiography (DSA) system to
image pharmacologically mediated changes in the vascular
system of the rat. Imaging physiological function in the
rodent calls for high spatial and temporal resolutions and
the use of a reproducible image acquisition chain. Dynamic
vascular images and quantitative perfusion metrics were
acquired before and after a vasoconstrictor, phenylephrine
drug injection. Dramatic effects of the vasoconstrictor on
vascular dynamics are seen in the prolonged blood flow mean
transit time, amount of ventricular filling, the size
increase of the pulmonary arteries and aorta, and a
substantial increase in mean arterial pressure. © 2006
IEEE.},
Key = {064610243353}
}
@article{9073128,
Author = {Ming De Lin and Hedlund, L. and Johnson,
G.A.},
Title = {Micro radiography imaging of the rodent with phenylephrine
induced vascular hypertension},
Journal = {2006 3rd IEEE International Symposium on Biomedical Imaging:
Macro to Nano (IEEE Cat. No.06EX1231C)},
Pages = {610 - 13},
Address = {Arlington, VA, USA},
Year = {2006},
Keywords = {blood vessels;diagnostic radiography;drugs;haemorheology;},
Abstract = {Vascular tonicity plays a major role in regulating the blood
pressure and the perfusion of organs, and hypertension is a
major cause of morbidity and mortality in humans. We use
phenylephrine, a vasoconstrictor, to create a model of
hypertension in the rat. This work demonstrates the use of a
micro-X-ray digital subtraction angiography (DSA) system to
image pharmacologically mediated changes in the vascular
system of the rat. Imaging physiological function in the
rodent calls for high spatial and temporal resolutions and
the use of a reproducible image acquisition chain. Dynamic
vascular images and quantitative perfusion metrics were
acquired before and after a vasoconstrictor, phenylephrine
drug injection. Dramatic effects of the vasoconstrictor on
vascular dynamics are seen in the prolonged blood flow mean
transit time, amount of ventricular filling, the size
increase of the pulmonary arteries and aorta, and a
substantial increase in mean arterial pressure},
Key = {9073128}
}
@article{fds268833,
Author = {Badea, CT and Hedlund, LW and Cook, J and Berridge, BR and Johnson,
GA},
Title = {Micro-CT imaging assessment of dobutamine-induced cardiac
stress in rats.},
Journal = {J Pharmacol Toxicol Methods},
Volume = {63},
Number = {1},
Pages = {24-29},
Year = {2011},
ISSN = {1873-488X},
url = {http://www.ncbi.nlm.nih.gov/pubmed/20399875},
Keywords = {Animals • Cardiac Output • Dobutamine •
Echocardiography • Electrocardiography • Heart
• Heart Rate • Heart Ventricles • Magnetic
Resonance Imaging • Male • Rats • Rats,
Sprague-Dawley • Stress, Physiological • X-Ray
Microtomography • administration & dosage • drug
effects • drug effects* • methods* •
pathology • pharmacology* • physiology •
radiography*},
Abstract = {INTRODUCTION: Dobutamine (DOB) stress in animal models of
heart disease has been imaged so far using echocardiography
and magnetic resonance imaging. The purpose of this study
was to assess normal response to DOB stress in rats using
anatomical and functional data using micro-computed
tomography (CT). METHODS: Ten normal adult male rats were
first injected with a liposomal-based blood pool contrast
agent and next infused with DOB via a tail vein catheter.
Using prospective gating, 5 pairs of systole/diastole
micro-CT images were acquired (a) pre-infusion baseline; (b)
at heart rate plateau during infusion of 10 μg/kg/min DOB;
(c) at post-DOB infusion baseline; (d) at heart rate plateau
during infusion of 30 μg/kg/min DOB; and (e) after
post-infusion return to baseline. Heart rate, peripheral and
breathing distensions were monitored by oximetry. Micro-CT
images with 88-μm isotropic voxels were segmented to obtain
cardiac function based on volumetric measurements of the
left ventricle. RESULTS: DOB stress increased heart rate and
cardiac output with both doses. Ejection fraction increased
above baseline by an average of 35.9% with the first DOB
dose and 18.4% with the second dose. No change was observed
in the relative peripheral arterial pressures associated
with the significant increases in cardiac output.
DISCUSSION: Micro-CT proved to be a robust imaging method
able to provide isotropic data on cardiac morphology and
function. Micro-CT has the advantage of being faster and
more cost-effective than MR and is able to provide higher
accuracy than echocardiography. The impact of such an
enabling technology can be enormous in evaluating
cardiotoxic effects of various test drugs.},
Language = {eng},
Doi = {10.1016/j.vascn.2010.04.002},
Key = {fds268833}
}
@article{fds132896,
Author = {C Badea and LW Hedlund and GA Johnson},
Title = {Micro-CT with respiratory and cardiac gating.},
Journal = {Medical physics, United States},
Volume = {31},
Number = {12},
Pages = {3324-9},
Year = {2004},
Month = {December},
ISSN = {0094-2405},
Keywords = {Animals • Artifacts • Electrocardiography •
Equipment Design • Equipment Failure Analysis* •
Heart • Lung • Mice • Mice, Inbred C57BL
• Movement* • Radiographic Image Enhancement
• Radiographic Image Interpretation, Computer-Assisted
• Respiratory Mechanics • Tomography, X-Ray
Computed • instrumentation* • methods •
radiography* • veterinary},
Abstract = {Cardiopulmonary imaging in rodents using micro-computed
tomography (CT) is a challenging task due to both cardiac
and pulmonary motion and the limited fluence rate available
from micro-focus x-ray tubes of most commercial systems.
Successful imaging in the mouse requires recognition of both
the spatial and temporal scales and their impact on the
required fluence rate. Smaller voxels require an increase in
the total number of photons (integrated fluence) used in the
reconstructed image for constant signal-to-noise ratio. The
faster heart rates require shorter exposures to minimize
cardiac motion blur imposing even higher demands on the
fluence rate. We describe a system with fixed tube/detector
and with a rotating specimen. A large focal spot x-ray tube
capable of producing high fluence rates with short exposure
times was used. The geometry is optimized to match focal
spot blur with detector pitch and the resolution limits
imposed by the reproducibility of gating. Thus, it is
possible to achieve isotropic spatial resolution of 100
microm with a fluence rate at the detector 250 times that of
a conventional cone beam micro-CT system with rotating
detector and microfocal x-ray tube. Motion is minimized for
any single projection with 10 ms exposures that are
synchronized to both cardiac and breathing motion. System
performance was validated in vivo by studies of the
cardiopulmonary structures in C57BL/6 mice, demonstrating
the value of motion integration with a bright x-ray
source.},
Key = {fds132896}
}
@booklet{Badea04,
Author = {Badea, C and Hedlund, LW and Johnson, GA},
Title = {Micro-CT with respiratory and cardiac gating.},
Journal = {Med Phys},
Volume = {31},
Number = {12},
Pages = {3324-3329},
Year = {2004},
Month = {December},
ISSN = {0094-2405},
url = {http://www.ncbi.nlm.nih.gov/pubmed/15651615},
Abstract = {Cardiopulmonary imaging in rodents using micro-computed
tomography (CT) is a challenging task due to both cardiac
and pulmonary motion and the limited fluence rate available
from micro-focus x-ray tubes of most commercial systems.
Successful imaging in the mouse requires recognition of both
the spatial and temporal scales and their impact on the
required fluence rate. Smaller voxels require an increase in
the total number of photons (integrated fluence) used in the
reconstructed image for constant signal-to-noise ratio. The
faster heart rates require shorter exposures to minimize
cardiac motion blur imposing even higher demands on the
fluence rate. We describe a system with fixed tube/detector
and with a rotating specimen. A large focal spot x-ray tube
capable of producing high fluence rates with short exposure
times was used. The geometry is optimized to match focal
spot blur with detector pitch and the resolution limits
imposed by the reproducibility of gating. Thus, it is
possible to achieve isotropic spatial resolution of 100
microm with a fluence rate at the detector 250 times that of
a conventional cone beam micro-CT system with rotating
detector and microfocal x-ray tube. Motion is minimized for
any single projection with 10 ms exposures that are
synchronized to both cardiac and breathing motion. System
performance was validated in vivo by studies of the
cardiopulmonary structures in C57BL/6 mice, demonstrating
the value of motion integration with a bright x-ray
source.},
Doi = {10.1118/1.1812604},
Key = {Badea04}
}
@article{fds357200,
Author = {Badea, A and Schmalzigaug, R and Kim, W and Bonner, P and Ahmed, U and Johnson, GA and Cofer, G and Foster, M and Anderson, RJ and Badea, C and Premont, RT},
Title = {Microcephaly with altered cortical layering in GIT1
deficiency revealed by quantitative neuroimaging.},
Journal = {Magn Reson Imaging},
Volume = {76},
Pages = {26-38},
Year = {2021},
Month = {February},
url = {http://dx.doi.org/10.1016/j.mri.2020.09.023},
Abstract = {G Protein-Coupled Receptor Kinase-Interacting Protein-1
(GIT1) regulates neuronal functions, including cell and axon
migration and synapse formation and maintenance, and GIT1
knockout (KO) mice exhibit learning and memory deficits. We
noted that male and female GIT1-KO mice exhibit neuroimaging
phenotypes including microcephaly, and altered cortical
layering, with a decrease in neuron density in cortical
layer V. Micro-CT and magnetic resonance microscopy (MRM)
were used to identify morphometric phenotypes for the skulls
and throughout the GIT1-KO brains. High field MRM of
actively-stained mouse brains from GIT1-KO and wild type
(WT) controls (n = 6 per group) allowed segmenting 37
regions, based on co-registration to the Waxholm Space
atlas. Overall brain size in GIT1-KO mice was ~32% smaller
compared to WT controls. After correcting for brain size,
several regions were significantly different in GIT1-KO mice
relative to WT, including the gray matter of the ventral
thalamic nuclei and the rest of the thalamus, the inferior
colliculus, and pontine nuclei. GIT1-KO mice had reduced
volume of white matter tracts, most notably in the anterior
commissure (~26% smaller), but also in the cerebral
peduncle, fornix, and spinal trigeminal tract. On the other
hand, the basal ganglia appeared enlarged in GIT1-KO mice,
including the globus pallidus, caudate putamen, and
particularly the accumbens - supporting a possible
vulnerability to addiction. Volume based morphometry based
on high-resolution MRM (21.5 μm isotropic voxels) was
effective in detecting overall, and local differences in
brain volumes in GIT1-KO mice, including in white matter
tracts. The reduced relative volume of specific brain
regions suggests a critical, but not uniform, role for GIT1
in brain development, conducive to brain microcephaly, and
aberrant connectivity.},
Doi = {10.1016/j.mri.2020.09.023},
Key = {fds357200}
}
@article{fds268807,
Author = {Jiang, Y and Johnson, GA},
Title = {Microscopic diffusion tensor atlas of the mouse
brain.},
Journal = {Neuroimage},
Volume = {56},
Number = {3},
Pages = {1235-1243},
Year = {2011},
Month = {June},
ISSN = {1095-9572},
url = {http://www.ncbi.nlm.nih.gov/pubmed/21419226},
Keywords = {Analysis of Variance • Animals • Atlases as Topic*
• Brain • Databases, Factual • Diffusion
Tensor Imaging • Image Processing, Computer-Assisted
• Informatics • Internet • Male • Mice
• Mice, Inbred C57BL • anatomy & histology* •
methods*},
Abstract = {Eight diffusion tensor imaging (DTI) datasets of normal
adult C57BL/6J mouse brains were acquired with an isotropic
Nyquist limited resolution of 43 μm (voxel volume ~80 pl).
Each specimen was scanned with a b0 image and 6
diffusion-weighted images. T1- and T2*-weighted data were
acquired with each specimen to aid nonlinear registration of
the data to a common reference space (called "Waxholm
Space"). We identified 80 different discrete landmarks in
Waxholm Space to provide the gold standard for measuring the
registration quality. The accuracy of the registration was
established by measuring displacement of the 80 landmarks in
each registered brain from the same landmarks in the
reference brain. The accuracy was better than 130 μm for
95% of the landmarks (overall landmark displacement is
65±40 μm, n=640). Mean and coefficient of variation
atlases of DTI indices were generated with potential
application for both voxel-based and region of
interest-based analysis. To examine consistency of DTI data
among individual subjects in this study and difference in
diffusion indices between separate brain structures within
each subject, averaged values of DTI indices (axial
diffusivity, radial diffusivity, fractional anisotropy, and
angular deviation of the primary eigenvector) were computed
in 9 white matter structures in each brain. The variation of
the DTI indices across the population was very small, e.g.,
~5% for axial diffusivity for each white matter structure,
enabling confident differentiation of differences in these
structures within each subject. ANOVA tests indicated that
the current protocol is able to provide consistent DTI data
of individual brains (p>0.25), and distinguish difference of
diffusion indices between white matter structures (p<0.001).
Power analysis was also performed to provide an estimate of
the number of specimens required to detect a 10% change of
the DTI indices in each white matter structure. The data
provide a critical addition to Waxholm Space, the
International Neuroinformatics Coordinating Facility
(www.incf.org) online comprehensive atlas of the mouse
brain.},
Language = {eng},
Doi = {10.1016/j.neuroimage.2011.03.031},
Key = {fds268807}
}
@article{fds268837,
Author = {Jiang, Y and Johnson, GA},
Title = {Microscopic diffusion tensor imaging of the mouse
brain.},
Journal = {Neuroimage},
Volume = {50},
Number = {2},
Pages = {465-471},
Year = {2010},
Month = {April},
ISSN = {1095-9572},
url = {http://www.ncbi.nlm.nih.gov/pubmed/20034583},
Keywords = {Animals • Anisotropy • Brain • Brain Mapping
• Diffusion Tensor Imaging • Image Interpretation,
Computer-Assisted • Mice • Staining and Labeling
• Tissue Fixation • anatomy & histology* •
methods • methods*},
Abstract = {Diffusion tensor imaging (DTI) data at 43 mum isotropic
resolution has been acquired on the intact adult mouse brain
in 28-h scan time by using a streamlined protocol, including
specimen fixation and staining, image acquisition,
reconstruction, post-processing, and distribution. An
intermediate registration of each component image is
required to achieve the desired microscopic resolution.
Multiple parameters have been derived, including fractional
anisotropy, axial and radial diffusivity, and a color-coded
orientation map of the primary eigenvector. Each DTI dataset
was mapped to a common reference space to facilitate future
standardized analysis. Fiber tracking has also been
demonstrated, providing 3D connection information. This
protocol to acquire high-resolution DTI data in a robust and
repeatable fashion will serve as a foundation to
quantitatively study mouse brain integrity and white matter
architecture, at what we believe to be the highest spatial
resolution yet attained.},
Language = {eng},
Doi = {10.1016/j.neuroimage.2009.12.057},
Key = {fds268837}
}
@article{fds132747,
Author = {G.A. Johnson},
Title = {Miller JR, Hurlston SE, Face DW, Kountz DJ, MacFall JR,
Hedlund LW, Ma QY, Johnson GA. Performance of a
high-temperature superconducting probe for in vivo microsopy
at 2.0 T Magn Reson Med. In press 1998},
Year = {1998},
Key = {fds132747}
}
@booklet{Johnson93b,
Author = {G. A. Johnson and J. T. Handa and C. A. Baker and G. J.
Jaffe},
Title = {Minoxidil inhibits human rpe cell-growth by a mechanism
dependent on the conversion to minoxidil
sulfate},
Journal = {Investigative Ophthalmology \& Visual Science},
Volume = {34},
Number = {4},
Pages = {1426 -- 1426},
Year = {1993},
Month = {March},
Key = {Johnson93b}
}
@booklet{Fiedlerweiss87,
Author = {V. C. Fiedlerweiss and G. A. Johnson and C. M. Buys and C.
A. Baker},
Title = {Minoxidil sulfate and sulfotransferase activity in
alopecia-areata},
Journal = {Clinical Research},
Volume = {35},
Number = {3},
Pages = {A682 -- A682},
Year = {1987},
Month = {April},
Key = {Fiedlerweiss87}
}
@article{fds174250,
Author = {AE Buhl and DJ Waldon and CA Baker and GA Johnson},
Title = {Minoxidil sulfate is the active metabolite that stimulates
hair follicles.},
Journal = {The Journal of investigative dermatology},
Volume = {95},
Number = {5},
Pages = {553-7},
Year = {1990},
Month = {November},
ISSN = {0022-202X},
Keywords = {Acetaminophen • Animals • Cells, Cultured •
Chlorates • Diethylcarbamazine • Hair • Mice
• Minoxidil • Sulfotransferases • Vibrissae
• analogs & derivatives* • antagonists &
inhibitors • cytology • drug effects • drug
effects* • enzymology • metabolism •
pharmacology},
Abstract = {An important step in understanding minoxidil's mechanism of
action on hair follicles was to determine the drug's active
form. We used organ-cultured vibrissa follicles to test
whether it is minoxidil or its sulfated metabolite,
minoxidil sulfate, that stimulates hair growth. Follicles
from neonatal mice were cultured with or without drugs and
effects were assessed by measuring incorporation of
radiolabeled cysteine in hair shafts of the treated
follicles. Assays of minoxidil sulfotransferase activity
indicated that vibrissae follicles metabolize minoxidil to
minoxidil sulfate. Dose-response studies showed that
minoxidil sulfate is 14 times more potent than minoxidil in
stimulating cysteine incorporation in cultured follicles.
Three drugs that block production of intrafollicular
minoxidil sulfate were tested for their effects on
drug-induced hair growth. Diethylcarbamazine proved to be a
noncompetitive inhibitor of sulfotransferase and prevented
hair growth stimulation by minoxidil but not by minoxidil
sulfate. Inhibiting the formation of intracellular PAPS with
chlorate also blocked the action of minoxidil but not of
minoxidil sulfate. Acetaminophen, a potent sulfate scavenger
blocked cysteine incorporation by minoxidil. It also blocked
follicular stimulation by minoxidil sulfate apparently by
directly removing the sulfate from the drug. Experiments
with U-51,607, a potent minoxidil analog that also forms a
sulfated metabolite, showed that its activity was inhibited
by both chlorate and diethylcarbamazine. These studies show
that sulfation is a critical step for hair-growth effects of
minoxidil and that it is the sulfated metabolite that
directly affects hair follicles.},
Language = {eng},
Key = {fds174250}
}
@booklet{Buhl90,
Author = {A. E. Buhl and D. J. Waldon and C. A. Baker and G. A.
Johnson},
Title = {Minoxidil sulfate is the active metabolite that stimulates
hair-follicles},
Journal = {Journal Of Investigative Dermatology},
Volume = {95},
Number = {5},
Pages = {553 -- 557},
Year = {1990},
Month = {November},
Key = {Buhl90}
}
@booklet{Johnson83c,
Author = {G. A. Johnson and K. J. Barsuhn and J. M.
Mccall},
Title = {Minoxidil sulfate, a metabolite of minoxidil},
Journal = {Drug Metabolism And Disposition},
Volume = {11},
Number = {5},
Pages = {507 -- 508},
Year = {1983},
Key = {Johnson83c}
}
@article{fds132908,
Author = {GA Johnson and KJ Barsuhn and JM McCall},
Title = {Minoxidil sulfate, a metabolite of minoxidil.},
Journal = {Drug metabolism and disposition: the biological fate of
chemicals, UNITED STATES},
Volume = {11},
Number = {5},
Pages = {507-8},
ISSN = {0090-9556},
Keywords = {Animals • Bile • Chromatography, High Pressure
Liquid • Female • Minoxidil • Pyrimidines
• Rats • Rats, Inbred Strains • analogs &
derivatives • metabolism • metabolism*},
Key = {fds132908}
}
@booklet{Baker94,
Author = {C. A. Baker and H. Uno and G. A. Johnson},
Title = {Minoxidil sulfation in the hair follicle},
Journal = {Skin Pharmacology},
Volume = {7},
Number = {6},
Pages = {335 -- 339},
Year = {1994},
Key = {Baker94}
}
@article{fds174125,
Author = {CA Baker and H Uno and GA Johnson},
Title = {Minoxidil sulfation in the hair follicle.},
Journal = {Skin pharmacology : the official journal of the Skin
Pharmacology Society},
Volume = {7},
Number = {6},
Pages = {335-9},
Year = {1994},
ISSN = {1011-0283},
Keywords = {Animals • Female • Hair • Macaca •
Minoxidil • Sulfates • Sulfotransferases •
metabolism • metabolism*},
Abstract = {The in vivo model which may be the most accurate for the
ability to predict hair growth in humans, and which was
utilized in the preclinical development of minoxidil, is the
adult stumptailed macaque. Previous reports have suggested
that the enzyme activity which accounts for the activation
of minoxidil, i.e., minoxidil sulfotransferase, is present
in skin. We have demonstrated that scalp skin from the
stumptailed macaque contains minoxidil sulfotransferase
activity, and further with dissection of that scalp skin
into epidermis, dermis and hair follicle, most of
sulfotransferase activity was present in the follicle.
Sulfotransferase activity in the hair follicle in
freeze-dried scalp skin sections from 9 stumptailed macaques
ranged from 47 to 84% of the total (mean 61 +/- 12%). Much
less minoxidil sulfotransferase activity was measured in the
epidermis (mean 18 +/- 11%, with a range of 2-37%) and the
dermis (mean 21 +/- 8%, with a range of 4-35%) of these
scalp sections. These results indicate that the scalp skin
from the stumptailed macaque contains minoxidil
sulfotransferase activity and this activity is largely
localized in the hair follicle which may account for its
ability to stimulate hair growth in this animal
model.},
Language = {eng},
Key = {fds174125}
}
@booklet{Baker91,
Author = {C. A. Baker and H. Uno and G. A. Johnson},
Title = {Minoxidil sulfotransferase activity in hair-follicles},
Journal = {Clinical Research},
Volume = {39},
Number = {2},
Pages = {A537 -- A537},
Year = {1991},
Month = {April},
Key = {Baker91}
}
@booklet{Baker91a,
Author = {C. A. Baker and H. Uno and G. A. Johnson},
Title = {Minoxidil sulfotransferase activity in hair-follicles},
Journal = {Journal Of Investigative Dermatology},
Volume = {96},
Number = {4},
Pages = {576 -- 576},
Year = {1991},
Month = {April},
Key = {Baker91a}
}
@booklet{Buhl94,
Author = {A. E. Buhl and C. A. Baker and A. J. Dietz and F. T. Murray and G. A. Johnson},
Title = {Minoxidil sulfotransferase activity influences the efficacy
of rogaine(r) topical solutions (ts) - enzyme studies using
scalp and platelets},
Journal = {Journal Of Investigative Dermatology},
Volume = {102},
Number = {4},
Pages = {534 -- 534},
Year = {1994},
Month = {April},
Key = {Buhl94}
}
@booklet{Johnson92,
Author = {G. A. Johnson and C. A. Baker and K. A. Knight},
Title = {Minoxidil sulfotransferase, a marker of human keratinocyte
differentiation},
Journal = {Journal Of Investigative Dermatology},
Volume = {98},
Number = {5},
Pages = {730 -- 733},
Year = {1992},
Month = {May},
Key = {Johnson92}
}
@article{fds174244,
Author = {GA Johnson and CA Baker and KA Knight},
Title = {Minoxidil sulfotransferase, a marker of human keratinocyte
differentiation.},
Journal = {The Journal of investigative dermatology},
Volume = {98},
Number = {5},
Pages = {730-3},
Year = {1992},
Month = {May},
ISSN = {0022-202X},
Keywords = {Biological Markers • Calcium • Cell
Differentiation • Enzyme Induction • Humans •
Keratinocytes • Sulfotransferases • Time Factors
• Transglutaminases • cytology* • enzymology
• metabolism • pharmacology •
pharmacology*},
Abstract = {The sulfation of minoxidil is catalyzed by a
sulfotransferase activity in a number of tissues including
skin. To investigate further the nature of the minoxidil
sulfotransferase activity in epithelial tissue and to
compare this activity to that of cholesterol
sulfotransferase, which has already been shown to be induced
during the differentiation of epithelial cells, we cultured
normal human epidermal keratinocytes in a keratinocyte
growth medium for 4 d, after which the media were replaced
with either the same growth media or media with increasing
Ca++ concentrations. Cholesterol sulfotransferase, minoxidil
sulfotransferase, and transglutaminase were determined
during the differentiation of the cells in the three media.
Time-activity curves that suggested two different
sulfotransferase activities were induced during the
differentiation process. U-77581, a competitive inhibitor of
minoxidil sulfotransferase activity, inhibited the sulfation
of minoxidil sulfotransferase activity in the keratinocyte
homogenates, but it did not inhibit the sulfation of
cholesterol. These data indicate that at least two
sulfotransferase activities are induced during the
differentiation of epithelial keratinocytes and minoxidil
sulfotransferase is an early marker of that
differentiation.},
Language = {eng},
Key = {fds174244}
}
@article{fds268988,
Author = {Hedlund, LW and Möller, HE and Chen, XJ and Chawla, MS and Cofer, GP and Johnson, GA},
Title = {Mixing oxygen with hyperpolarized (3)He for small-animal
lung studies.},
Journal = {NMR Biomed},
Volume = {13},
Number = {4},
Pages = {202-206},
Year = {2000},
Month = {June},
ISSN = {0952-3480},
url = {http://www.ncbi.nlm.nih.gov/pubmed/10867697},
Keywords = {Animals • Animals, Laboratory • Helium* •
Intubation, Intratracheal • Isotopes • Lung •
Magnetic Resonance Imaging • Oxygen* • Rats •
Respiratory Function Tests • anatomy & histology •
instrumentation • methods* • physiology*},
Abstract = {Hyperpolarized helium (HP (3)He) is useful for direct MR
imaging of the gas spaces of small animal lungs. Previously,
breaths of 100% HP (3)He were alternated with breaths of air
to maximize helium signal in the lungs and to minimize the
depolarizing effects of O(2). However, for high-resolution
imaging requiring many HP (3)He breaths (hundreds) and for
pulmonary disease studies, a method was needed to
simultaneously deliver O(2) and HP (3)He with each breath
without significant loss of polarization. We modified our
existing computer-controlled ventilator by adding a plastic
valve, additional relays and a controller. O(2) and HP (3)He
are mixed at the beginning of each breath within the body of
a breathing valve, which is attached directly to the
endotracheal tube. With this mixing method, we found that
T(1) relaxation of HP (3)He in the guinea pig lung was about
20 s compared to 30 s with alternate air/HP (3)He breathing.
Because imaging times during each breath are short (about
500 ms), the HP (3)He signal loss from O(2) contact is
calculated to be less than 5%. We concluded that the
advantages of mixing HP (3)He with O(2), such as shorter
imaging times (reduced T(1) losses in reservoir) and
improved physiologic stability, outweigh the small signal
loss from the depolarizing effects of oxygen on HP
(3)He.},
Doi = {10.1002/1099-1492(200006)13:4<202::aid-nbm645>3.0.co;2-j},
Key = {fds268988}
}
@booklet{Hedlund00a,
Author = {L. W. Hedlund and H. E. Moller and X. J. Chen and M. S.
Chawla and G. P. Cofer and G. A. Johnson},
Title = {Mixing oxygen with hyperpolarized He-3 for small-animal lung
studies},
Journal = {Nmr In Biomedicine},
Volume = {13},
Number = {4},
Pages = {202 -- 206},
Year = {2000},
Month = {June},
Key = {Hedlund00a}
}
@booklet{Kim03,
Author = {J. G. Kim and J. H. Song and J. L. Vallet and G. A. Rohrer and G. A. Johnson and M. M. Joyce and R. K.
Christenson},
Title = {Molecular characterization and expression of porcine bone
morphogenetic protein receptor-IB in the uterus of cyclic
and pregnant gilts},
Journal = {Biology Of Reproduction},
Volume = {68},
Number = {3},
Pages = {735 -- 743},
Year = {2003},
Month = {March},
Key = {Kim03}
}
@article{fds174173,
Author = {JG Kim and JH Song and JL Vallet and GA Rohrer and GA Johnson and MM Joyce and RK Christenson},
Title = {Molecular characterization and expression of porcine bone
morphogenetic protein receptor-IB in the uterus of cyclic
and pregnant gilts.},
Journal = {Biology of reproduction},
Volume = {68},
Number = {3},
Pages = {735-43},
Year = {2003},
Month = {March},
ISSN = {0006-3363},
Keywords = {Amino Acid Sequence • Animals • Base Sequence
• Blotting, Northern • Bone Morphogenetic Protein
Receptors, Type I • Chromosome Mapping • Cloning,
Molecular • Crosses, Genetic • DNA, Complementary
• Endometrium • Expressed Sequence Tags •
Female • Gene Expression Regulation • In Situ
Hybridization • Male • Molecular Sequence Data
• Point Mutation • Pregnancy • Pregnancy,
Animal • Protein-Serine-Threonine Kinases • RNA,
Messenger • Receptors, Growth Factor • Sequence
Alignment • Sequence Analysis, DNA • Swine •
biosynthesis • chemistry • genetics •
genetics* • isolation & purification • metabolism
• metabolism* • physiology •
veterinary},
Abstract = {Previous gene mapping analyses revealed a quantitative trait
locus for uterine capacity on chromosome 8. Comparison of
porcine and human genetic maps suggests that the bone
morphogenetic protein receptor IB (BMPR-IB) gene may be
located near this region. The objectives of this study were
to 1) clone the full coding region for BMPR-IB, 2) examine
BMPR-IB gene expression by the endometrium and its cellular
localization in cyclic and pregnant gilts, and 3) map the
BMPR-IB gene. By iterative screening of an expressed
sequence tag library, we obtained a 3559-base pair cDNA
clone including the full coding region of BMPR-IB.
Endometrial BMPR-IB mRNA expression of White composite gilts
was determined by Northern blotting in Days 10, 13, and 15
cyclic and Days 10, 13, 15, 20, 30, and 40 pregnant gilts.
In cyclic gilts, endometrial BMPR-IB mRNA expression was
elevated on Days 13 and 15 (P < 0.01) compared with Day 10.
Expression of BMPR-IB mRNA was localized in both luminal and
glandular epithelium on Day 15. However, in pregnant gilts,
BMPR-IB mRNA expression was not significantly different in
the endometrium from Day 10 to Day 20, and it was
significantly decreased on Days 30 and 40 (P = 0.011). The
BMPR-IB gene was mapped to 108 cM on chromosome 8. These
findings show that BMPR-IB mRNA expression is regulated
differently in cyclic and pregnant gilts; this pattern of
gene expression may be important for endometrial function
during the luteal phase of the estrous cycle as compared
with early pregnancy.},
Language = {eng},
Key = {fds174173}
}
@article{fds174228,
Author = {E Asselin and GA Johnson and TE Spencer and FW Bazer},
Title = {Monocyte chemotactic protein-1 and -2 messenger ribonucleic
acids in the ovine uterus: regulation by pregnancy,
progesterone, and interferon-tau.},
Journal = {Biology of reproduction},
Volume = {64},
Number = {3},
Pages = {992-1000},
Year = {2001},
Month = {March},
ISSN = {0006-3363},
Keywords = {Animals • Chemokine CCL2 • Chemokine CCL8 •
Endometrium • Estrus • Female •
Histocytochemistry • Image Processing,
Computer-Assisted • Interferon Type I • Monocyte
Chemoattractant Proteins • Nucleic Acid Hybridization
• Pregnancy • Pregnancy Proteins • Pregnancy,
Animal • Progesterone • RNA, Messenger •
Random Allocation • Recombinant Proteins • Reverse
Transcriptase Polymerase Chain Reaction • Sheep •
Steroids • biosynthesis • cytology • genetics
• genetics* • metabolism • metabolism* •
pharmacology • pharmacology* • physiology* •
veterinary},
Abstract = {Endometrial leukocytes may play important roles during
pregnancy. Because chemokines are regulators of immune cell
activity and trafficking, this study determined if mRNAs for
monocyte chemotactic proteins (MCP) were present in the
ovine uterus and regulated by progesterone (P) and/or
recombinant ovine interferon tau (roIFN-tau). Uteri of
normal cycling and pregnant ewes (experiment 1) and uteri of
ovariectomized ewes receiving intrauterine infusions of
IFN-tau and/or i.m. injections of P (experiment 2) were used
to detect MCP-1 and MCP-2 mRNA. In experiment 1, slot-blot
hybridization analysis of endometrial total RNA revealed
that MCP-1 and MCP-2 mRNA levels did not change during the
estrous cycle but increased between Days 13 and 19 of
pregnancy. Using in situ hybridization, MCP-1 and MCP-2 mRNA
were localized to immune cells in the subepithelial compact
stroma. Histomorphological studies and in situ hybridization
for major basic protein (MBP) indicated that MCP-positive
immune cells were eosinophils. In experiment 2, treatment
with P and roIFN-tau increased (P < 0.05) the number of
MCP-1- and MCP-2-expressing eosinophils in the endometrium
compared to ewes treated with P alone. Injection of the P
receptor antagonist (ZK 137,316) inhibited effects of P
and/or roIFN-tau to recruit eosinophils expressing MCP-1 and
MCP-2 mRNAs. Endometrial production of MCPs by eosinophils
during early pregnancy may play a role(s) in central
implantation and/or placentation in ewes that is crucial for
successful establishment of pregnancy.},
Language = {eng},
Key = {fds174228}
}
@booklet{Asselin01,
Author = {E. Asselin and G. A. Johnson and T. E. Spencer and F. W.
Bazer},
Title = {Monocyte chemotactic protein-1 and-2 messenger ribonucleic
acids in the ovine uterus: Regulation by pregnancy,
progesterone, and interferon-tau},
Journal = {Biology Of Reproduction},
Volume = {64},
Number = {3},
Pages = {992 -- 1000},
Year = {2001},
Month = {March},
Key = {Asselin01}
}
@booklet{Johnson02g,
Author = {G. A. Johnson and G. P. Cofer and S. L. Gewalt and L. W.
Hedlund},
Title = {Morphologic phenotyping with MR microscopy: The visible
mouse},
Journal = {Radiology},
Volume = {222},
Number = {3},
Pages = {789 -- 793},
Year = {2002},
Month = {March},
Key = {Johnson02g}
}
@article{fds268929,
Author = {Johnson, GA and Cofer, GP and Gewalt, SL and Hedlund,
LW},
Title = {Morphologic phenotyping with MR microscopy: the visible
mouse.},
Journal = {Radiology},
Volume = {222},
Number = {3},
Pages = {789-793},
Year = {2002},
Month = {March},
ISSN = {0033-8419},
url = {http://www.ncbi.nlm.nih.gov/pubmed/11867802},
Keywords = {Animals • Contrast Media • Gadolinium DTPA •
Imaging, Three-Dimensional* • Magnetic Resonance
Imaging • Male • Mice • Mice, Inbred C57BL
• Mice, Knockout • Microscopy • Phenotype
• Urate Oxidase • administration & dosage •
anatomy & histology* • diagnostic use • genetics
• methods*},
Abstract = {A method for rapid morphologic phenotyping is demonstrated
by using magnetic resonance microscopy. Whole fixed C57BL/6J
mice were imaged at 110-microm isotropic resolution; limited
volumes of the intact specimen, at 50-microm isotropic
resolution; and isolated organs, at 25-microm isotropic
resolution. The three-dimensional imaging technique was
applied to uricase knockout mice to demonstrate the method
for the evaluation of morphologic phenotype.},
Doi = {10.1148/radiol.2223010531},
Key = {fds268929}
}
@article{fds268812,
Author = {Perperidis, D and Bucholz, E and Johnson, GA and Constantinides,
C},
Title = {Morphological studies of the murine heart based on
probabilistic and statistical atlases.},
Journal = {Comput Med Imaging Graph},
Volume = {36},
Number = {2},
Pages = {119-129},
Year = {2012},
Month = {March},
ISSN = {1879-0771},
url = {http://www.ncbi.nlm.nih.gov/pubmed/21820867},
Keywords = {Algorithms* • Animals • Contrast Media • Data
Interpretation, Statistical • Gadolinium DTPA •
Heart • Image Enhancement • Image Interpretation,
Computer-Assisted • Magnetic Resonance Imaging •
Male • Mice • Mice, Inbred C57BL •
Reproducibility of Results • Sensitivity and
Specificity • Subtraction Technique* • anatomy &
histology* • diagnostic use* • methods •
methods*},
Abstract = {This study directly compares morphological features of the
mouse heart in its end-relaxed state based on constructed
morphometric maps and atlases using principal component
analysis in C57BL/6J (n=8) and DBA (n=5) mice. In
probabilistic atlases, a gradient probability exists for
both strains in longitudinal locations from base to apex.
Based on the statistical atlases, differences in size
(49.8%), apical direction (15.6%), basal ventricular blood
pool size (13.2%), and papillary muscle shape and position
(17.2%) account for the most significant modes of shape
variability for the left ventricle of the C57BL/6J mice. For
DBA mice, differences in left ventricular size and direction
(67.4%), basal size (15.7%), and position of papillary
muscles (16.8%) account for significant variability.},
Language = {eng},
Doi = {10.1016/j.compmedimag.2011.07.001},
Key = {fds268812}
}
@article{fds268851,
Author = {Hedlund, LW and Johnson, GA},
Title = {Morphology of the small-animal lung using magnetic resonance
microscopy.},
Journal = {Proc Am Thorac Soc},
Volume = {2},
Number = {6},
Pages = {481-502},
Year = {2005},
ISSN = {1546-3222},
url = {http://www.ncbi.nlm.nih.gov/pubmed/16352752},
Keywords = {Animals • Artifacts • Image Processing,
Computer-Assisted • Lung • Magnetic Resonance
Imaging* • Mice • Microscopy* • Rats •
pathology*},
Abstract = {Small-animal imaging with magnetic resonance microscopy
(MRM) has become an important tool in biomedical research.
When MRM is used to image perfusion-fixed and "stained"
whole mouse specimens, cardiopulmonary morphology can be
visualized, nondestructively, in exquisite detail in all
three dimensions. This capability can be a valuable tool for
morphologic phenotyping of different mouse strains commonly
used in genomics research. When these imaging techniques are
combined with specialized methods for biological motion
control and animal support, the lungs of the live, small
animal can be imaged. Although in vivo imaging may not
achieve the high resolution possible with a fixed specimen,
dynamic functional studies and survival studies that follow
the progression of pulmonary change related to disease or
environmental exposure are possible. By combining
conventional proton imaging with gas imaging, using
hyperpolarized 3He, it is possible to image the tissue and
gas compartments of the lung. This capability is illustrated
in studies on an emphysema model in rats and on radiation
damage of the lung. With further improvements in imaging and
animal handling technology, we will be able to image faster
and at higher resolutions, making MRM an even more valuable
research tool.},
Doi = {10.1513/pats.200507-074DS},
Key = {fds268851}
}
@article{fds268869,
Author = {Badea, A and Ali-Sharief, AA and Johnson, GA},
Title = {Morphometric analysis of the C57BL/6J mouse
brain.},
Journal = {Neuroimage},
Volume = {37},
Number = {3},
Pages = {683-693},
Year = {2007},
Month = {September},
ISSN = {1053-8119},
url = {http://www.ncbi.nlm.nih.gov/pubmed/17627846},
Keywords = {Animals • Anthropometry • Brain • Image
Interpretation, Computer-Assisted • Imaging,
Three-Dimensional • Magnetic Resonance Imaging •
Mice • Mice, Inbred C57BL • Models, Anatomic*
• Models, Neurological* • anatomy & histology*
• methods*},
Abstract = {Magnetic resonance microscopy (MRM), when used in
conjunction with active staining, can produce
high-resolution, high-contrast images of the mouse brain.
Using MRM, we imaged in situ the fixed, actively stained
brains of C57BL/6J mice in order to characterize the
neuroanatomical phenotype and produce a digital atlas. The
brains were scanned within the cranium vault to preserve the
brain morphology, avoid distortions, and to allow an
unbiased shape analysis. The high-resolution imaging used a
T1-weighted scan at 21.5 microm isotropic resolution, and an
eight-echo multi-echo scan, post-processed to obtain an
enhanced T2 image at 43 microm resolution. The two image
sets were used to segment the brain into 33 anatomical
structures. Volume, area, and shape characteristics were
extracted for all segmented brain structures. We also
analyzed the variability of volumes, areas, and shape
characteristics. The coefficient of variation of volume had
an average value of 7.0%. Average anatomical images of the
brain for both the T1-weighted and T2 images were generated,
together with an average shape atlas, and a probabilistic
atlas for 33 major structures. These atlases, with their
associated meta-data, will serve as baseline for identifying
neuroanatomical phenotypes of additional strains, and mouse
models now under study. Our efforts were directed toward
creating a baseline for comparison with other mouse strains
and models of neurodegenerative diseases.},
Doi = {10.1016/j.neuroimage.2007.05.046},
Key = {fds268869}
}
@article{fds359547,
Author = {Cody, D and Stickel, J and Johnson, G and Boone, J},
Title = {MO‐D‐330D‐01: Molecular Imaging I: The Physics of
Molecular Imaging},
Journal = {Medical Physics},
Volume = {33},
Number = {6},
Pages = {2160},
Year = {2006},
Month = {January},
url = {http://dx.doi.org/10.1118/1.2241412},
Abstract = {The first day of the Molecular Imaging Symposium (MI‐1)
will focus on the technology aspects of molecular,
functional, and small animal imaging. Modalities which will
be discussed include micro‐CT, micro‐PET, and high
resolution MRI for small animal imaging. The presentation on
micro‐CT technology will include an introduction to the
basic requirements of the scanner hardware, examples of the
images and biological applications in which micro‐CT is
useful, and the radiation dose to the small animal
undergoing micro‐CT will also be discussed. Micro‐CT
techniques require longer acquisition times than human
scanners, and thus maintaining the animal in a viable but
motionless state is of clear importance. Therefore, issues
surrounding animal support including anesthesia and
respiratory gating will also be presented. Micro‐PET
systems are widely used in small animal imaging for genome
research, and represent probably the mainstay of truly
molecular imaging modalities at this point in time. The
presentation on micro‐PET will include a description of
micro‐PET scanner hardware, a discussion of
PET‐radiotracers, and an overview of current small animal
PET systems. The limitation of current micro‐PET system
design will be discussed, and ideas for overcoming some of
these limitations will be presented. High resolution MRI
systems have the benefit of delivering exquisite contrast
with excellent spatial resolution, with no ionizing
radiation. The presentation on micro‐MRI techniques for
phenotype imaging will describe the integration of physics,
biology, chemistry, engineering, and computer science which
is necessary to achieve state‐of‐the‐art small animal
MRI imaging. The use of hyperpolarized gases for lung
imaging and MR histology will be discussed as well. The
availability of small animal imaging systems across a number
of modalities has proved essential for a large number of
research applications. The primary goal of MI‐1 is to help
familiarize medical physicists with the technical design and
capabilities of these high resolution small animal imaging
systems, and to highlight research applications of their
use. Different modalities are used to address different
research questions, and this symposium will emphasize the
strengths and weaknesses of each modality in regards to
various research applications. Differences between animal
imaging and human scanners will also be discussed. © 2006,
American Association of Physicists in Medicine. All rights
reserved.},
Doi = {10.1118/1.2241412},
Key = {fds359547}
}
@booklet{Hedlund86,
Author = {Hedlund, LW and Johnson, GA and Karis, JP and Effmann,
EL},
Title = {MR "microscopy" of the rat thorax.},
Journal = {J Comput Assist Tomogr},
Volume = {10},
Number = {6},
Pages = {948-952},
Year = {1986},
ISSN = {0363-8715},
url = {http://www.ncbi.nlm.nih.gov/pubmed/3782566},
Abstract = {High resolution images can be obtained from anywhere in the
body of small animals with magnetic resonance combined with
cardiac gating and scan synchronous ventilation. We used
these methods to examine the intrathoracic anatomy of the
rat. Anesthetized rats were intubated and ventilated in
synchrony with imaging acquisition. Images were obtained in
a 1 m bore, 1.5 T system fitted with a 28 cm diameter high
field gradient coil and a 5 cm radio-frequency coil. We used
cardiac gated, three-dimensional spin warp acquisitions.
Eight contiguous slices (2.5 mm thick) were obtained
simultaneously. In addition to visualizing major vessels and
cardiac chambers, cardiac valves and papillary muscles were
clearly demonstrated. Major pulmonary vessels and peripheral
parenchyma were also seen. These results demonstrate MR
"microscopy" can be used to image all major cardiopulmonary
structures in the rat with respect to selected times of the
cardiac cycle. This capability for noninvasive "microscopy"
opens new avenues for cardiopulmonary research using well
characterized rodent models.},
Doi = {10.1097/00004728-198611000-00010},
Key = {Hedlund86}
}
@article{fds132890,
Author = {LW Hedlund and GA Johnson and JP Karis and EL Effmann},
Title = {MR "microscopy" of the rat thorax.},
Journal = {Journal of computer assisted tomography, UNITED
STATES},
Volume = {10},
Number = {6},
Pages = {948-52},
ISSN = {0363-8715},
Keywords = {Animals • Electrocardiography • Fourier Analysis
• Magnetic Resonance Spectroscopy • Male •
Rats • Rats, Inbred Strains • Thorax •
anatomy & histology* • diagnostic use* •
instrumentation • methods},
Abstract = {High resolution images can be obtained from anywhere in the
body of small animals with magnetic resonance combined with
cardiac gating and scan synchronous ventilation. We used
these methods to examine the intrathoracic anatomy of the
rat. Anesthetized rats were intubated and ventilated in
synchrony with imaging acquisition. Images were obtained in
a 1 m bore, 1.5 T system fitted with a 28 cm diameter high
field gradient coil and a 5 cm radio-frequency coil. We used
cardiac gated, three-dimensional spin warp acquisitions.
Eight contiguous slices (2.5 mm thick) were obtained
simultaneously. In addition to visualizing major vessels and
cardiac chambers, cardiac valves and papillary muscles were
clearly demonstrated. Major pulmonary vessels and peripheral
parenchyma were also seen. These results demonstrate MR
"microscopy" can be used to image all major cardiopulmonary
structures in the rat with respect to selected times of the
cardiac cycle. This capability for noninvasive "microscopy"
opens new avenues for cardiopulmonary research using well
characterized rodent models.},
Key = {fds132890}
}
@article{fds268759,
Author = {MacFall, JR and Benveniste, H and Maki, J and Johnson, GA and Hedlund,
L and Copher, G},
Title = {MR diffusion measurements in stroke models in rat
brains},
Journal = {Proceedings of the Annual Conference on Engineering in
Medicine and Biology},
Number = {pt 1},
Pages = {67-68},
Year = {1990},
Month = {December},
Abstract = {It is shown that tissue self-diffusion coefficients can be
calculated from a series of diffusion-weighted MR (magnetic
resonance) images. By variation of the time between
diffusion sensitizing gradient pulses, the motions of tissue
water may be characterized on varying length scales from 10
μm to 100 μm. Typically, the diffusion coefficient
decreases as this diffusion time increases. Such
characterization of tissue water properties may be of use in
the study of the course of pathology in disease
models.},
Key = {fds268759}
}
@booklet{Drayer86b,
Author = {DRAYER, B and BURGER, P and CAIN, J and LEONG, J and JOHNSON, GA and HEINZ,
ER and RIEDERER, S and DJANG, W and HERFKENS, R},
Title = {MR IMAGING AND PERLS STAIN OF BASAL GANGLIA IRON WITH NORMAL
AGING},
Journal = {AMERICAN JOURNAL OF NEURORADIOLOGY},
Volume = {7},
Number = {3},
Pages = {554-555},
Publisher = {AMER SOC NEURORADIOLOGY},
Year = {1986},
Month = {May},
ISSN = {0195-6108},
url = {http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:A1986C133300130&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=47d3190e77e5a3a53558812f597b0b92},
Key = {Drayer86b}
}
@booklet{Maki91,
Author = {Maki, JH and Benveniste, H and MacFall, JR and Piantadosi, CA and Johnson, GA},
Title = {MR imaging of microcirculation in rat brain: correlation
with carbon dioxide-induced changes in blood
flow.},
Journal = {J Magn Reson Imaging},
Volume = {1},
Number = {6},
Pages = {673-681},
Year = {1991},
ISSN = {1053-1807},
url = {http://www.ncbi.nlm.nih.gov/pubmed/1823172},
Abstract = {Considerable interest has been shown in developing a
magnetic resonance (MR) imaging technique with quantitative
capability in the evaluation of tissue microcirculation
("perfusion"). In the present study, the
flow-dephased/flow-compensated (FD/FC) technique is
evaluated for measuring rat cerebral blood flow (CBF) under
nearly optimal laboratory conditions. Imaging was performed
on a 2.0-T system equipped with shielded gradient coils. Rat
CBF was varied by manipulating arterial carbon dioxide
pressure (PaCO2). In parallel experiments, optimized MR
imaging studies (seven rats) were compared with laser
Doppler flowmetry (LDF) studies (nine rats). LDF values
showed a high degree of correlation between CBF and PaCO2,
agreeing with results in the literature. MR imaging values,
while correlating with PaCO2, showed considerable scatter.
The most likely explanation is unavoidable rat motion during
the requisite long imaging times. Because of this motion
sensitivity, the FD/FC technique cannot provide a
quantitative measure of CBF. It can, however, provide a
qualitative picture.},
Doi = {10.1002/jmri.1880010609},
Key = {Maki91}
}
@article{fds132785,
Author = {JH Maki and H Benveniste, JR MacFall and CA Piantadosi and GA
Johnson},
Title = {MR imaging of microcirculation in rat brain: correlation
with carbon dioxide-induced changes in blood
flow.},
Journal = {Journal of magnetic resonance imaging : JMRI, UNITED
STATES},
Volume = {1},
Number = {6},
Pages = {673-81},
ISSN = {1053-1807},
Keywords = {Animals • Artifacts • Brain • Carbon Dioxide
• Cerebral Cortex • Cerebrovascular Circulation
• Corpus Striatum • Female • Image
Enhancement • Lasers • Linear Models •
Magnetic Resonance Imaging • Microcirculation •
Models, Structural • Motion • Rats • Rats,
Inbred Strains • blood supply • blood supply*
• blood* • diagnostic use • methods* •
physiology*},
Abstract = {Considerable interest has been shown in developing a
magnetic resonance (MR) imaging technique with quantitative
capability in the evaluation of tissue microcirculation
("perfusion"). In the present study, the
flow-dephased/flow-compensated (FD/FC) technique is
evaluated for measuring rat cerebral blood flow (CBF) under
nearly optimal laboratory conditions. Imaging was performed
on a 2.0-T system equipped with shielded gradient coils. Rat
CBF was varied by manipulating arterial carbon dioxide
pressure (PaCO2). In parallel experiments, optimized MR
imaging studies (seven rats) were compared with laser
Doppler flowmetry (LDF) studies (nine rats). LDF values
showed a high degree of correlation between CBF and PaCO2,
agreeing with results in the literature. MR imaging values,
while correlating with PaCO2, showed considerable scatter.
The most likely explanation is unavoidable rat motion during
the requisite long imaging times. Because of this motion
sensitivity, the FD/FC technique cannot provide a
quantitative measure of CBF. It can, however, provide a
qualitative picture.},
Key = {fds132785}
}
@article{fds132757,
Author = {CE Spritzer and JB Vogler and S Martinez and WE Garrett and GA Johnson and MJ McNamara and J Lohnes and RJ Herfkens},
Title = {MR imaging of the knee: preliminary results with a 3DFT
GRASS pulse sequence.},
Journal = {AJR. American journal of roentgenology, UNITED
STATES},
Volume = {150},
Number = {3},
Pages = {597-603},
Year = {1988},
Month = {March},
ISSN = {0361-803X},
Keywords = {Adolescent • Adult • Arthroscopy • Female
• Humans • Joint Diseases • Knee Injuries
• Knee Joint • Magnetic Resonance Imaging* •
Male • Middle Aged • anatomy & histology •
diagnosis • methods • pathology*},
Abstract = {The knees of 17 patients (18 extremities) with possible
meniscal, cruciate ligament, and articular cartilage
abnormalities were examined with a three-dimensional Fourier
transform (3DFT), gradient-refocused acquisition in a steady
state (GRASS) pulse sequence. Arthroscopic confirmation was
available in all cases and was the standard for comparison.
Thirteen of these extremities were also examined by using a
two-dimensional Fourier transform spin-echo pulse sequence
with a 2000-msec repetition time and 20- and 80-msec echo
time. In these 13 cases, both pulse sequences correctly
identified seven of eight meniscal abnormalities. However,
interpretation of the 3DFT GRASS images resulted in fewer
false-positive meniscal tears (three vs six). Cruciate
ligament tears were detected more readily on the 3DFT GRASS
images (six vs three with two possible tears on the
spin-echo images). These preliminary findings suggest that
the overall accuracy of MR imaging of the knee could be
improved by including 3DFT gradient-refocused pulse
sequences.},
Key = {fds132757}
}
@booklet{Spritzer88,
Author = {Spritzer, CE and Vogler, JB and Martinez, S and Garrett, WE and Johnson,
GA and McNamara, MJ and Lohnes, J and Herfkens, RJ},
Title = {MR imaging of the knee: preliminary results with a 3DFT
GRASS pulse sequence.},
Journal = {AJR Am J Roentgenol},
Volume = {150},
Number = {3},
Pages = {597-603},
Year = {1988},
Month = {March},
ISSN = {0361-803X},
url = {http://www.ncbi.nlm.nih.gov/pubmed/3257617},
Abstract = {The knees of 17 patients (18 extremities) with possible
meniscal, cruciate ligament, and articular cartilage
abnormalities were examined with a three-dimensional Fourier
transform (3DFT), gradient-refocused acquisition in a steady
state (GRASS) pulse sequence. Arthroscopic confirmation was
available in all cases and was the standard for comparison.
Thirteen of these extremities were also examined by using a
two-dimensional Fourier transform spin-echo pulse sequence
with a 2000-msec repetition time and 20- and 80-msec echo
time. In these 13 cases, both pulse sequences correctly
identified seven of eight meniscal abnormalities. However,
interpretation of the 3DFT GRASS images resulted in fewer
false-positive meniscal tears (three vs six). Cruciate
ligament tears were detected more readily on the 3DFT GRASS
images (six vs three with two possible tears on the
spin-echo images). These preliminary findings suggest that
the overall accuracy of MR imaging of the knee could be
improved by including 3DFT gradient-refocused pulse
sequences.},
Doi = {10.2214/ajr.150.3.597},
Key = {Spritzer88}
}
@article{fds269110,
Author = {Middleton, H and Black, RD and Saam, B and Cates, GD and Cofer, GP and Guenther, R and Happer, W and Hedlund, LW and Johnson, GA and Juvan,
K},
Title = {MR imaging with hyperpolarized 3He gas.},
Journal = {Magn Reson Med},
Volume = {33},
Number = {2},
Pages = {271-275},
Year = {1995},
Month = {February},
ISSN = {0740-3194},
url = {http://www.ncbi.nlm.nih.gov/pubmed/7707920},
Keywords = {Animals • Guinea Pigs • Helium • Image
Enhancement • Lasers • Lung • Magnetic
Resonance Imaging* • Magnetic Resonance Spectroscopy
• Rubidium • Time Factors • anatomy &
histology* • chemistry • diagnostic use* •
metabolism • methods • radiation
effects},
Abstract = {Magnetic resonance images of the lungs of a guinea pig have
been produced using hyperpolarized helium as the source of
the MR signal. The resulting images are not yet sufficiently
optimized to reveal fine structural detail within the lung,
but the spectacular signal from this normally
signal-deficient organ system offers great promise for
eventual in vivo imaging experiments. Fast 2D and 3D GRASS
sequences with very small flip angles were employed to
conserve the norenewable longitudinal magnetization. We
discuss various unique features associated with performing
MRI with hyperpolarized gases, such as the selection of the
noble gas species, polarization technique, and constraints
on the MR pulse sequence.},
Doi = {10.1002/mrm.1910330219},
Key = {fds269110}
}
@article{fds132777,
Author = {MD Shattuck and SL Gewalt and GH Glover and LW Hedlund and GA
Johnson},
Title = {MR microimaging of the lung using volume projection
encoding.},
Journal = {Magnetic resonance in medicine : official journal of the
Society of Magnetic Resonance in Medicine / Society of
Magnetic Resonance in Medicine, UNITED STATES},
Volume = {38},
Number = {6},
Pages = {938-42},
Year = {1997},
Month = {December},
ISSN = {0740-3194},
Keywords = {Animals • Guinea Pigs • Lung • Magnetic
Resonance Imaging • anatomy & histology* •
methods*},
Abstract = {Radial acquisition (RA) techniques have been extended to
produce isotropic, three-dimensional images of lung in live
laboratory animals at spatial resolution down to 0.013 mm3
with a signal-to-noise ratio of 30:1. The pulse sequence and
reconstruction algorithm have been adapted to allow
acquisition of image matrices of up to 256(3) in less than
15 min. Scan-synchronous ventilation has been incorporated
to limit breathing motion artifacts. The imaging sequence
permits randomizing and/or discarding selected views to
minimize the consequences of breathing motion. The signal in
lung parenchyma was measured as a function of flip angle
(alpha) for different repetition times and found to follow
the predictions for which there is an optimum excitation
(Ernst) angle. A single T1 relaxation value of 780 +/- 54 ms
fits all data from six guinea pigs at 2.0 T. This T1 value
parameterizes the signal and allows for a priori
optimization, such as calculation of the Ernst angle
appropriate for lung imaging.},
Key = {fds132777}
}
@booklet{Shattuck97,
Author = {Shattuck, MD and Gewalt, SL and Glover, GH and Hedlund, LW and Johnson,
GA},
Title = {MR microimaging of the lung using volume projection
encoding.},
Journal = {Magn Reson Med},
Volume = {38},
Number = {6},
Pages = {938-942},
Year = {1997},
Month = {December},
ISSN = {0740-3194},
url = {http://www.ncbi.nlm.nih.gov/pubmed/9402195},
Abstract = {Radial acquisition (RA) techniques have been extended to
produce isotropic, three-dimensional images of lung in live
laboratory animals at spatial resolution down to 0.013 mm3
with a signal-to-noise ratio of 30:1. The pulse sequence and
reconstruction algorithm have been adapted to allow
acquisition of image matrices of up to 256(3) in less than
15 min. Scan-synchronous ventilation has been incorporated
to limit breathing motion artifacts. The imaging sequence
permits randomizing and/or discarding selected views to
minimize the consequences of breathing motion. The signal in
lung parenchyma was measured as a function of flip angle
(alpha) for different repetition times and found to follow
the predictions for which there is an optimum excitation
(Ernst) angle. A single T1 relaxation value of 780 +/- 54 ms
fits all data from six guinea pigs at 2.0 T. This T1 value
parameterizes the signal and allows for a priori
optimization, such as calculation of the Ernst angle
appropriate for lung imaging.},
Doi = {10.1002/mrm.1910380613},
Key = {Shattuck97}
}
@booklet{Malisch91,
Author = {Malisch, TW and Hedlund, LW and Suddarth, SA and Johnson,
GA},
Title = {MR microscopy at 7.0 T: effects of brain
iron.},
Journal = {J Magn Reson Imaging},
Volume = {1},
Number = {3},
Pages = {301-305},
Year = {1991},
ISSN = {1053-1807},
url = {http://www.ncbi.nlm.nih.gov/pubmed/1802143},
Abstract = {The T2 of brain tissue is known to be field dependent,
decreasing as B0 increases. Previous studies have attributed
reduced T2 in the structures of the extrapyramidal motor
system (EPMS) to high iron concentrations. The present study
was designed to manipulate physiologic iron concentrations
and study the effects on T2 and on the field dependence of
T2 at 7.0 T in whole formalin-fixed brains. A rat model was
devised in which iron concentrations in the structures of
interest were altered by diet manipulation. Cerebral
structures with different iron content were imaged and T2
measured with MR microscopy at both 2.0 and 7.0 T. T2 of all
tissues was shorter by 40%-60% at 7.0 T. Although some
dependence of T2 on iron concentration was evident, it was
less than expected. The strongest correlation was in the
substantia nigra. The highest-resolution studies, at 30 x 30
x 50 microns, show the myelin bundles in many of the EPMS
structures but not in the substantia nigra. From these data,
it appears that T2 at greater field strengths depends more
on susceptibility-induced spin dephasing imposed by
diffusion through the tissue microstructure than on the
presence of iron.},
Doi = {10.1002/jmri.1880010308},
Key = {Malisch91}
}
@article{fds132810,
Author = {TW Malisch and LW Hedlund and SA Suddarth and GA Johnson},
Title = {MR microscopy at 7.0 T: effects of brain
iron.},
Journal = {Journal of magnetic resonance imaging : JMRI, UNITED
STATES},
Volume = {1},
Number = {3},
Pages = {301-5},
ISSN = {1053-1807},
Keywords = {Animals • Brain Chemistry • Extrapyramidal Tracts
• Iron • Magnetic Resonance Imaging* • Rats
• Rats, Inbred Strains • Substantia Nigra •
anatomy & histology* • physiology*},
Abstract = {The T2 of brain tissue is known to be field dependent,
decreasing as B0 increases. Previous studies have attributed
reduced T2 in the structures of the extrapyramidal motor
system (EPMS) to high iron concentrations. The present study
was designed to manipulate physiologic iron concentrations
and study the effects on T2 and on the field dependence of
T2 at 7.0 T in whole formalin-fixed brains. A rat model was
devised in which iron concentrations in the structures of
interest were altered by diet manipulation. Cerebral
structures with different iron content were imaged and T2
measured with MR microscopy at both 2.0 and 7.0 T. T2 of all
tissues was shorter by 40%-60% at 7.0 T. Although some
dependence of T2 on iron concentration was evident, it was
less than expected. The strongest correlation was in the
substantia nigra. The highest-resolution studies, at 30 x 30
x 50 microns, show the myelin bundles in many of the EPMS
structures but not in the substantia nigra. From these data,
it appears that T2 at greater field strengths depends more
on susceptibility-induced spin dephasing imposed by
diffusion through the tissue microstructure than on the
presence of iron.},
Key = {fds132810}
}
@booklet{Smith92,
Author = {Smith, BR and Effmann, EL and Johnson, GA},
Title = {MR microscopy of chick embryo vasculature.},
Journal = {J Magn Reson Imaging},
Volume = {2},
Number = {2},
Pages = {237-240},
Year = {1992},
ISSN = {1053-1807},
url = {http://www.ncbi.nlm.nih.gov/pubmed/1562778},
Abstract = {Six-day-old chick embryos were examined with magnetic
resonance microscopy after vascular perfusion fixation and
perfusion with gadolinium-doped gelatin to high-light the
developing vascular anatomy. Gadolinium gelatin, with its
short T1, provided a source of signal contrast within the
vessels. The entire embryo was embedded in gelatin to
minimize susceptibility artifacts that are prevalent at the
high field strength (7.0 T) used. A series of single-section
spin-echo images were acquired with various TRs to determine
the optimal imaging sequence for a three-dimensional (3D)
acquisition. The combination of gadolinium gelatin in the
vascular spaces, gelatin embedding of the specimen, and
optimal acquisition parameters yielded a 3D stack of
high-resolution images that was readily reconstructed and
rendered to effectively demonstrate the developing thoracic
vessels in the embryo.},
Doi = {10.1002/jmri.1880020220},
Key = {Smith92}
}
@article{fds132846,
Author = {BR Smith and EL Effmann and GA Johnson},
Title = {MR microscopy of chick embryo vasculature.},
Journal = {Journal of magnetic resonance imaging : JMRI, UNITED
STATES},
Volume = {2},
Number = {2},
Pages = {237-40},
ISSN = {1053-1807},
Keywords = {Animals • Blood Vessels • Chick Embryo •
Magnetic Resonance Imaging • embryology* •
methods*},
Abstract = {Six-day-old chick embryos were examined with magnetic
resonance microscopy after vascular perfusion fixation and
perfusion with gadolinium-doped gelatin to high-light the
developing vascular anatomy. Gadolinium gelatin, with its
short T1, provided a source of signal contrast within the
vessels. The entire embryo was embedded in gelatin to
minimize susceptibility artifacts that are prevalent at the
high field strength (7.0 T) used. A series of single-section
spin-echo images were acquired with various TRs to determine
the optimal imaging sequence for a three-dimensional (3D)
acquisition. The combination of gadolinium gelatin in the
vascular spaces, gelatin embedding of the specimen, and
optimal acquisition parameters yielded a 3D stack of
high-resolution images that was readily reconstructed and
rendered to effectively demonstrate the developing thoracic
vessels in the embryo.},
Key = {fds132846}
}
@article{fds268952,
Author = {Chen, XJ and Chawla, MS and Hedlund, LW and Möller, HE and MacFall, JR and Johnson, GA},
Title = {MR microscopy of lung airways with hyperpolarized
3He.},
Journal = {Magn Reson Med},
Volume = {39},
Number = {1},
Pages = {79-84},
Year = {1998},
Month = {January},
ISSN = {0740-3194},
url = {http://www.ncbi.nlm.nih.gov/pubmed/9438440},
Keywords = {Animals • Disease Models, Animal • Guinea Pigs
• Helium • Humans • Image Enhancement •
Isotopes • Lung • Magnetic Resonance Imaging
• Male • Microscopy • Middle Aged •
Pulmonary Gas Exchange • Reference Values •
Sensitivity and Specificity • anatomy & histology*
• diagnostic use* • methods • methods* •
physiology},
Abstract = {A technique using hyperpolarized (HP) 3He to image the small
airways of the lung by using moderate flip angles and a
short scanning period during early inspiration is
demonstrated. Flip angles (alpha) ranging from 10-90 degrees
were used in guinea pig experiments with scanning during the
entire inspiration period. A second series acquired data
throughout a short window of the ventilatory cycle with
alpha = 45 degrees. The success of the animal studies has
motivated implementation of similar imaging techniques in
the clinical arena. Human studies involved imaging over the
total inspiration period with alpha approximately 10
degrees. The first series of guinea pig experiments
demonstrated that larger flip angles (50-90 degrees) destroy
the magnetization before it reaches the smaller airways. At
moderate flip angles (20-40 degrees), airway branching down
to the fourth generation was apparent. Fifth-order
branchings were seen in the images of the second series. The
trachea down to fourth generation pulmonary airway
branching, along with some distal air spaces, was seen in
the human lung images.},
Doi = {10.1002/mrm.1910390113},
Key = {fds268952}
}
@booklet{Chen98a,
Author = {X. J. Chen and M. S. Chawla and L. W. Hedlund and H. E.
Moller and J. R. Macfall and G. A. Johnson},
Title = {MR microscopy of lung airways with hyperpolarized
He-3},
Journal = {Magnetic Resonance In Medicine},
Volume = {39},
Number = {1},
Pages = {79 -- 84},
Year = {1998},
Month = {January},
Key = {Chen98a}
}
@booklet{Farmer88,
Author = {FARMER, THR and JOHNSON, GA and COFER, GP and MARONPOT, RR and HEDLUND,
LW},
Title = {MR MICROSCOPY OF NEPHROTOXIC ACUTE TUBULAR-NECROSIS IN THE
RAT},
Journal = {INVESTIGATIVE RADIOLOGY},
Volume = {23},
Number = {9},
Pages = {S10-S10},
Publisher = {LIPPINCOTT-RAVEN PUBL},
Year = {1988},
Month = {September},
ISSN = {0020-9996},
url = {http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:A1988Q574500048&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=47d3190e77e5a3a53558812f597b0b92},
Key = {Farmer88}
}
@article{fds132862,
Author = {RM Summers and LW Hedlund and GP Cofer and MB Gottsman and JF Manibo and GA
Johnson},
Title = {MR microscopy of the rat carotid artery after balloon injury
by using an implanted imaging coil.},
Journal = {Magnetic resonance in medicine : official journal of the
Society of Magnetic Resonance in Medicine / Society of
Magnetic Resonance in Medicine, UNITED STATES},
Volume = {33},
Number = {6},
Pages = {785-9},
Year = {1995},
Month = {June},
ISSN = {0740-3194},
Keywords = {Angioplasty, Balloon • Animals • Carotid Artery
Injuries • Carotid Artery, External • Carotid
Stenosis • Female • Hyperplasia • Magnetic
Resonance Imaging* • Microscopy • Rats •
Rats, Sprague-Dawley • Tunica Intima • adverse
effects* • pathology • pathology* •
therapy},
Abstract = {Neointimal hyperplasia after angioplasty was followed in
vivo in rats by using MR microscopy and surgically implanted
RF imaging coils. By using an inductively coupled pick-up
coil, the arteries were imaged 4 days before and 3, 7, and
14 days after angioplasty with a 3DFT spin echo sequence.
Eight of 10 angioplastied rats showed moderate to severe
stensois based MR measures of lumen diameter reduction from
baseline images. There was a good correlation between total
wall thickness between MR and hematoxylin and eosin
(H&E)-stained sections obtained on the last day. Arteries in
the intact and sham groups remained unchanged from baseline
measurements. Because this imaging technique examines the
artery under in vivo conditions of arterial pressure and
flow, it promises to be a useful tool for evaluating
pharmacological and mechanical methods of reducing the
incidence of vascular stenosis.},
Key = {fds132862}
}
@booklet{Summers95,
Author = {Summers, RM and Hedlund, LW and Cofer, GP and Gottsman, MB and Manibo,
JF and Johnson, GA},
Title = {MR microscopy of the rat carotid artery after balloon injury
by using an implanted imaging coil.},
Journal = {Magn Reson Med},
Volume = {33},
Number = {6},
Pages = {785-789},
Year = {1995},
Month = {June},
ISSN = {0740-3194},
url = {http://www.ncbi.nlm.nih.gov/pubmed/7651114},
Abstract = {Neointimal hyperplasia after angioplasty was followed in
vivo in rats by using MR microscopy and surgically implanted
RF imaging coils. By using an inductively coupled pick-up
coil, the arteries were imaged 4 days before and 3, 7, and
14 days after angioplasty with a 3DFT spin echo sequence.
Eight of 10 angioplastied rats showed moderate to severe
stensois based MR measures of lumen diameter reduction from
baseline images. There was a good correlation between total
wall thickness between MR and hematoxylin and eosin
(H&E)-stained sections obtained on the last day. Arteries in
the intact and sham groups remained unchanged from baseline
measurements. Because this imaging technique examines the
artery under in vivo conditions of arterial pressure and
flow, it promises to be a useful tool for evaluating
pharmacological and mechanical methods of reducing the
incidence of vascular stenosis.},
Doi = {10.1002/mrm.1910330607},
Key = {Summers95}
}
@article{fds132886,
Author = {SL Gewalt and GH Glover and LW Hedlund and GP Cofer, JR MacFall and GA
Johnson},
Title = {MR microscopy of the rat lung using projection
reconstruction.},
Journal = {Magnetic resonance in medicine : official journal of the
Society of Magnetic Resonance in Medicine / Society of
Magnetic Resonance in Medicine, UNITED STATES},
Volume = {29},
Number = {1},
Pages = {99-106},
Year = {1993},
Month = {January},
ISSN = {0740-3194},
Keywords = {Animals • Female • Lung • Magnetic Resonance
Imaging* • Microscopy • Rats • Rats,
Sprague-Dawley • cytology*},
Abstract = {Projection reconstruction has been implemented with
self-refocused selection pulses on a small bore, 2.0 T MR
microscope, to allow imaging of lung parenchyma. Scan
synchronous ventilation and cardiac gating have been
integrated with the sequence to minimize motion artifacts. A
systematic survey of the pulse sequence parameters has been
undertaken in conjunction with the biological gating
parameters to optimize resolution and signal-to-noise (SNR).
The resulting projection images with effective echo time of
< 300 microseconds allow definition of lung parenchyma with
an SNR improvement of approximately 15 x over a more
conventional 2DFT short echo gradient sequence.},
Key = {fds132886}
}
@booklet{Gewalt93,
Author = {Gewalt, SL and Glover, GH and Hedlund, LW and Cofer, GP and MacFall, JR and Johnson, GA},
Title = {MR microscopy of the rat lung using projection
reconstruction.},
Journal = {Magn Reson Med},
Volume = {29},
Number = {1},
Pages = {99-106},
Year = {1993},
Month = {January},
ISSN = {0740-3194},
url = {http://www.ncbi.nlm.nih.gov/pubmed/8419748},
Abstract = {Projection reconstruction has been implemented with
self-refocused selection pulses on a small bore, 2.0 T MR
microscope, to allow imaging of lung parenchyma. Scan
synchronous ventilation and cardiac gating have been
integrated with the sequence to minimize motion artifacts. A
systematic survey of the pulse sequence parameters has been
undertaken in conjunction with the biological gating
parameters to optimize resolution and signal-to-noise (SNR).
The resulting projection images with effective echo time of
< 300 microseconds allow definition of lung parenchyma with
an SNR improvement of approximately 15 x over a more
conventional 2DFT short echo gradient sequence.},
Doi = {10.1002/mrm.1910290117},
Key = {Gewalt93}
}
@booklet{Maki88a,
Author = {MAKI, JH and COFER, GP and HEDLUND, LW and MACFALL, JR and JOHNSON,
GA},
Title = {MR MICROSCOPY USING DRIVEN EQUILIBRIUM (DEFT)},
Journal = {INVESTIGATIVE RADIOLOGY},
Volume = {23},
Number = {9},
Pages = {S21-S21},
Publisher = {LIPPINCOTT-RAVEN PUBL},
Year = {1988},
Month = {September},
ISSN = {0020-9996},
url = {http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:A1988Q574500093&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=47d3190e77e5a3a53558812f597b0b92},
Key = {Maki88a}
}
@booklet{Hedlund00,
Author = {Hedlund, LW and Cofer, GP and Owen, SJ and Allan Johnson,
G},
Title = {MR-compatible ventilator for small animals:
computer-controlled ventilation for proton and noble gas
imaging.},
Journal = {Magn Reson Imaging},
Volume = {18},
Number = {6},
Pages = {753-759},
Year = {2000},
Month = {July},
ISSN = {0730-725X},
url = {http://www.ncbi.nlm.nih.gov/pubmed/10930785},
Abstract = {We describe an MR-compatible ventilator that is computer
controlled to generate a variety of breathing patterns, to
minimize image degrading effects of breathing motion, and to
support delivery of gas anesthesia and experimental
inhalational gases. A key feature of this ventilator is the
breathing valve that attaches directly to the endotracheal
tube to reduce dead volume and allows independent control of
inspiratory and expiratory phases of ventilation. This
ventilator has been used in a wide variety of MR and x-ray
microscopy studies of small animals, especially for MR
imaging the lungs with hyperpolarized gases ((3)He &
(129)Xe).},
Doi = {10.1016/s0730-725x(00)00154-5},
Key = {Hedlund00}
}
@booklet{Middleton95,
Author = {H. Middleton and R. D. Black and B. Saam and G. D. Cates and G. P. Cofer and R. Guenther and W. Happer and L. W. Hedlund and G. A. Johnson and K. Juvan and J. Swartz},
Title = {Mr-imaging with hyperpolarized he-3 gas},
Journal = {Magnetic Resonance In Medicine},
Volume = {33},
Number = {2},
Pages = {271 -- 275},
Year = {1995},
Month = {February},
Key = {Middleton95}
}
@booklet{Bone86a,
Author = {BONE, SN and JOHNSON, GA and THOMPSON, MB},
Title = {MRI MICROSCOPY OF THE DEVELOPING CHICK-EMBRYO},
Journal = {INVESTIGATIVE RADIOLOGY},
Volume = {21},
Number = {9},
Pages = {S34-S34},
Publisher = {LIPPINCOTT-RAVEN PUBL},
Year = {1986},
Month = {September},
ISSN = {0020-9996},
url = {http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:A1986E000900143&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=47d3190e77e5a3a53558812f597b0b92},
Key = {Bone86a}
}
@article{fds132822,
Author = {B Drayer and P Burger and R Darwin and S Riederer and R Herfkens and GA
Johnson},
Title = {MRI of brain iron.},
Journal = {AJR. American journal of roentgenology, UNITED
STATES},
Volume = {147},
Number = {1},
Pages = {103-10},
Year = {1986},
Month = {July},
ISSN = {0361-803X},
Keywords = {Adolescent • Adult • Aged • Aging •
Brain • Brain Chemistry* • Brain Diseases •
Child • Globus Pallidus • Humans • Iron
• Magnetic Resonance Spectroscopy • Male •
Middle Aged • Substantia Nigra • analysis •
analysis* • diagnostic use* • metabolism •
pathology},
Abstract = {A prominently decreased signal intensity in the globus
pallidum, reticular substantia nigra, red nucleus, and
dentate nucleus was routinely noted in 150 consecutive
individuals on T2-weighted images (SE 2000/100) using a high
field strength (1.5 T)MR system. This MR finding correlated
closely with the decreased estimated T2 relaxation times and
the sites of preferential accumulation of ferric iron using
the Perls staining method on normal postmortem brains. The
decreased signal intensity on T2-weighted images thus
provides an accurate in vivo map of the normal distribution
of brain iron. Perls stain and MR studies in normal brain
also confirm an intermediate level of iron distribution in
the striatum, and still lower levels in the cerebral gray
and white matter. In the white matter, iron concentration is
(a) absent in the most posterior portion of the internal
capsule and optic radiations, (b) higher in the frontal than
occipital regions, and (c) prominent in the subcortical "U"
fibers, particularly in the temporal lobe. There is no iron
in the brain at birth; it increases progressively with
aging. Knowledge of the distribution of brain iron should
assist in elucidating normal anatomic structures and in
understanding neurodegenerative, demyelinating, and
cerebrovascular disorders.},
Key = {fds132822}
}
@booklet{Drayer86,
Author = {Drayer, B and Burger, P and Darwin, R and Riederer, S and Herfkens, R and Johnson, GA},
Title = {MRI of brain iron.},
Journal = {AJR Am J Roentgenol},
Volume = {147},
Number = {1},
Pages = {103-110},
Year = {1986},
Month = {July},
ISSN = {0361-803X},
url = {http://www.ncbi.nlm.nih.gov/pubmed/3487201},
Abstract = {A prominently decreased signal intensity in the globus
pallidum, reticular substantia nigra, red nucleus, and
dentate nucleus was routinely noted in 150 consecutive
individuals on T2-weighted images (SE 2000/100) using a high
field strength (1.5 T)MR system. This MR finding correlated
closely with the decreased estimated T2 relaxation times and
the sites of preferential accumulation of ferric iron using
the Perls staining method on normal postmortem brains. The
decreased signal intensity on T2-weighted images thus
provides an accurate in vivo map of the normal distribution
of brain iron. Perls stain and MR studies in normal brain
also confirm an intermediate level of iron distribution in
the striatum, and still lower levels in the cerebral gray
and white matter. In the white matter, iron concentration is
(a) absent in the most posterior portion of the internal
capsule and optic radiations, (b) higher in the frontal than
occipital regions, and (c) prominent in the subcortical "U"
fibers, particularly in the temporal lobe. There is no iron
in the brain at birth; it increases progressively with
aging. Knowledge of the distribution of brain iron should
assist in elucidating normal anatomic structures and in
understanding neurodegenerative, demyelinating, and
cerebrovascular disorders.},
Doi = {10.2214/ajr.147.1.103},
Key = {Drayer86}
}
@article{fds132808,
Author = {HE Möller and XJ Chen and B Saam and KD Hagspiel and GA Johnson and TA
Altes, EE de Lange and HU Kauczor},
Title = {MRI of the lungs using hyperpolarized noble
gases.},
Journal = {Magnetic resonance in medicine : official journal of the
Society of Magnetic Resonance in Medicine / Society of
Magnetic Resonance in Medicine, United States},
Volume = {47},
Number = {6},
Pages = {1029-51},
Year = {2002},
Month = {June},
ISSN = {0740-3194},
Keywords = {Animals • Forecasting • Helium • Humans
• Lung • Lung Diseases • Magnetic Resonance
Imaging • Noble Gases • Pulmonary Gas Exchange
• Xenon Radioisotopes • anatomy & histology*
• diagnosis* • diagnostic use • diagnostic
use* • methods* • physiology*},
Abstract = {The nuclear spin polarization of the noble gas isotopes
(3)He and (129)Xe can be increased using optical pumping
methods by four to five orders of magnitude. This
extraordinary gain in polarization translates directly into
a gain in signal strength for MRI. The new technology of
hyperpolarized (HP) gas MRI holds enormous potential for
enhancing sensitivity and contrast in pulmonary imaging.
This review outlines the physics underlying the optical
pumping process, imaging strategies coping with the
nonequilibrium polarization, and effects of the alveolar
microstructure on relaxation and diffusion of the noble
gases. It presents recent progress in HP gas MRI and
applications ranging from MR microscopy of airspaces to
imaging pulmonary function in patients and suggests
potential directions for future developments.},
Key = {fds132808}
}
@booklet{Moller02,
Author = {Möller, HE and Chen, XJ and Saam, B and Hagspiel, KD and Johnson, GA and Altes, TA and de Lange, EE and Kauczor, H-U},
Title = {MRI of the lungs using hyperpolarized noble
gases.},
Journal = {Magn Reson Med},
Volume = {47},
Number = {6},
Pages = {1029-1051},
Year = {2002},
Month = {June},
ISSN = {0740-3194},
url = {http://www.ncbi.nlm.nih.gov/pubmed/12111949},
Abstract = {The nuclear spin polarization of the noble gas isotopes
(3)He and (129)Xe can be increased using optical pumping
methods by four to five orders of magnitude. This
extraordinary gain in polarization translates directly into
a gain in signal strength for MRI. The new technology of
hyperpolarized (HP) gas MRI holds enormous potential for
enhancing sensitivity and contrast in pulmonary imaging.
This review outlines the physics underlying the optical
pumping process, imaging strategies coping with the
nonequilibrium polarization, and effects of the alveolar
microstructure on relaxation and diffusion of the noble
gases. It presents recent progress in HP gas MRI and
applications ranging from MR microscopy of airspaces to
imaging pulmonary function in patients and suggests
potential directions for future developments.},
Doi = {10.1002/mrm.10173},
Key = {Moller02}
}
@article{fds320199,
Author = {Xie, L and Bennett, KM and Liu, C and Johnson, GA and Zhang, JL and Lee,
VS},
Title = {MRI tools for assessment of microstructure and nephron
function of the kidney.},
Journal = {Am J Physiol Renal Physiol},
Volume = {311},
Number = {6},
Pages = {F1109-F1124},
Year = {2016},
Month = {December},
url = {http://dx.doi.org/10.1152/ajprenal.00134.2016},
Abstract = {MRI can provide excellent detail of renal structure and
function. Recently, novel MR contrast mechanisms and imaging
tools have been developed to evaluate microscopic kidney
structures including the tubules and glomeruli. Quantitative
MRI can assess local tubular function and is able to
determine the concentrating mechanism of the kidney
noninvasively in real time. Measuring single nephron
function is now a near possibility. In parallel to advancing
imaging techniques for kidney microstructure is a need to
carefully understand the relationship between the local
source of MRI contrast and the underlying physiological
change. The development of these imaging markers can impact
the accurate diagnosis and treatment of kidney disease. This
study reviews the novel tools to examine kidney
microstructure and local function and demonstrates the
application of these methods in renal pathophysiology.},
Doi = {10.1152/ajprenal.00134.2016},
Key = {fds320199}
}
@article{fds357205,
Author = {Holbrook, MD and Blocker, SJ and Mowery, YM and Badea, A and Qi, Y and Xu,
ES and Kirsch, DG and Johnson, GA and Badea, CT},
Title = {MRI-Based Deep Learning Segmentation and Radiomics of
Sarcoma in Mice.},
Journal = {Tomography},
Volume = {6},
Number = {1},
Pages = {23-33},
Year = {2020},
Month = {March},
url = {http://dx.doi.org/10.18383/j.tom.2019.00021},
Abstract = {Small-animal imaging is an essential tool that provides
noninvasive, longitudinal insight into novel cancer
therapies. However, considerable variability in image
analysis techniques can lead to inconsistent results. We
have developed quantitative imaging for application in the
preclinical arm of a coclinical trial by using a genetically
engineered mouse model of soft tissue sarcoma. Magnetic
resonance imaging (MRI) images were acquired 1 day before
and 1 week after radiation therapy. After the second MRI,
the primary tumor was surgically removed by amputating the
tumor-bearing hind limb, and mice were followed for up to
6 months. An automatic analysis pipeline was used for
multicontrast MRI data using a convolutional neural network
for tumor segmentation followed by radiomics analysis. We
then calculated radiomics features for the tumor, the
peritumoral area, and the 2 combined. The first radiomics
analysis focused on features most indicative of radiation
therapy effects; the second radiomics analysis looked for
features that might predict primary tumor recurrence. The
segmentation results indicated that Dice scores were similar
when using multicontrast versus single T2-weighted data
(0.863 vs 0.861). One week post RT, larger tumor volumes
were measured, and radiomics analysis showed greater
heterogeneity. In the tumor and peritumoral area, radiomics
features were predictive of primary tumor recurrence (AUC:
0.79). We have created an image processing pipeline for
high-throughput, reduced-bias segmentation of
multiparametric tumor MRI data and radiomics analysis, to
better our understanding of preclinical imaging and the
insights it provides when studying new cancer
therapies.},
Doi = {10.18383/j.tom.2019.00021},
Key = {fds357205}
}
@booklet{Johnson01,
Author = {G. A. Johnson and F. W. Bazer and L. A. Jaeger and H. Ka and J. E. Garlow and C. Pfarrer and T. E. Spencer and R. C.
Burghardt},
Title = {Muc-1, integrin, and osteopontin expression during the
implantation cascade in sheep},
Journal = {Biology Of Reproduction},
Volume = {65},
Number = {3},
Pages = {820 -- 828},
Year = {2001},
Month = {September},
Key = {Johnson01}
}
@article{fds174103,
Author = {GA Johnson and FW Bazer and LA Jaeger and H Ka and JE Garlow and C Pfarrer and TE Spencer and RC Burghardt},
Title = {Muc-1, integrin, and osteopontin expression during the
implantation cascade in sheep.},
Journal = {Biology of reproduction},
Volume = {65},
Number = {3},
Pages = {820-8},
Year = {2001},
Month = {September},
ISSN = {0006-3363},
Keywords = {Actinin • Animals • Cell Adhesion •
Cytoskeleton • Embryo Implantation • Endometrium
• Epithelium • Estrous Cycle • Female •
Fluorescent Antibody Technique • Gestational Age •
Immunohistochemistry • Integrins • Interferon Type
I • Mucin-1 • Osteopontin • Pregnancy •
Pregnancy Proteins • RNA, Messenger • Recombinant
Proteins • Reverse Transcriptase Polymerase Chain
Reaction • Sheep • Sialoglycoproteins • Talin
• Trophoblasts • Uterus • analysis •
analysis* • chemistry • genetics • genetics*
• pharmacology • physiology •
physiology*},
Abstract = {The extracellular matrix protein osteopontin (OPN) is a
component of histotroph that increases in uterine flushings
from pregnant ewes during the peri-implantation period and
is localized on the apical surfaces of the uterine luminal
epithelium (LE) and conceptus trophectoderm (Tr). The
potential involvement of OPN in the implantation adhesion
cascade in sheep was investigated by examining temporal,
spatial, and potential functional relationships between OPN,
Muc-1, and integrin subunits during the estrous cycle and
early pregnancy. Immunoreactive Muc-1 was highly expressed
at the apical surfaces of uterine luminal (LE) and glandular
epithelium (GE) in both cycling and pregnant ewes but was
decreased dramatically on LE by Day 9 and was nearly
undetectable by Day 17 of pregnancy when intimate contact
between LE and Tr begins. In contrast, integrin subunits
alpha(v), alpha(4), alpha(5), beta(1), beta(3), and beta(5)
were constitutively expressed on conceptus Tr and at the
apical surface of uterine LE and GE in both cyclic and early
pregnant ewes. The apical expression of these subunits could
contribute to the apical assembly of several OPN receptors
including the alpha(v)beta(3), alpha(v)beta(1),
alpha(v)beta(5), alpha(4)beta(1), and alpha(5)beta(1)
heterodimers on endometrial LE and GE, and conceptus Tr in
sheep. Functional analysis of potential OPN interactions
with conceptus and endometrial integrins was performed on LE
and Tr cells in vitro using beads coated with OPN,
poly-L-lysine, or recombinant OPN in which the Arg-Gly-Asp
sequence was replaced with RGE or RAD. Transmembrane
accumulation of talin or alpha-actinin at the apical surface
of uterine LE and conceptus Tr cells in contact with
OPN-coated beads revealed functional integrin activation and
cytoskeletal reorganization in response to OPN binding.
These results provide a physiological framework for the role
of OPN, a potential mediator of implantation in sheep, as a
bridge between integrin heterodimers expressed by Tr and
uterine LE responsible for adhesion for initial conceptus
attachment.},
Language = {eng},
Key = {fds174103}
}
@booklet{Paulson01,
Author = {Paulson, EK and Yoshizumi, TT and Frush, DP and Johnson,
GA},
Title = {Multi-detector vs single-detector CT: The organ doses are
higher than you think},
Journal = {RADIOLOGY},
Volume = {221},
Pages = {403-403},
Publisher = {RADIOLOGICAL SOC NORTH AMERICA},
Year = {2001},
Month = {November},
ISSN = {0033-8419},
url = {http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000172126601192&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=47d3190e77e5a3a53558812f597b0b92},
Key = {Paulson01}
}
@booklet{Nelson81,
Author = {Nelson, CE and Johnson, GA and Bentel, G},
Title = {MULTI-PLANAR RECONSTRUCTION–APPLICATIONS IN RADIATION
ONCOLOGY TREATMENT PLANNING},
Journal = {Investigative Radiology},
Volume = {16},
Number = {5},
Pages = {417-417},
Publisher = {Ovid Technologies (Wolters Kluwer Health)},
Year = {1981},
Month = {September},
ISSN = {0020-9996},
url = {http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:A1981MK56200113&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=47d3190e77e5a3a53558812f597b0b92},
Doi = {10.1097/00004424-198109000-00113},
Key = {Nelson81}
}
@article{064610243432,
Author = {Mistry, N and De Lin and M and Hedlund, L and Johnson,
GA},
Title = {Multimodality imaging of pulmonary function in the
rodent},
Journal = {2006 3rd IEEE International Symposium on Biomedical Imaging:
From Nano to Macro - Proceedings},
Volume = {2006},
Pages = {920-923},
Address = {Arlington, VA, United States},
Year = {2006},
Month = {November},
ISBN = {9780780395770},
Keywords = {Medical imaging;Optical resolving power;Physiological
models;Magnetic resonance imaging;Angiography;Imaging
systems;},
Abstract = {The high spatial and temporal resolution demands for imaging
physiological function in the rodent call for the use of
novel ways to combine information from different imaging
modalities. This work describes ventilation imaging using
hyperpolarized (HP) 3He magnetic resonance imaging (MRI) and
perfusion imaging using X-ray digital subtraction
angiography (DSA). We illustrate the key steps needed to
combine the complementary data from the two modalities to
provide qualitative and quantitative information on gas
exchange in the lungs. The results indicate that
multimodality imaging of pulmonary function in small animals
can provide functional information at higher spatial and
temporal resolution compared to many traditional imaging
techniques. © 2006 IEEE.},
Key = {064610243432}
}
@article{9054396,
Author = {Mistry, N. and Ming De Lin and Hedlund, L. and Johnson,
G.A.},
Title = {Multimodality imaging of pulmonary function in the
rodent},
Journal = {2006 3rd IEEE International Symposium on Biomedical Imaging:
Macro to Nano (IEEE Cat. No.06EX1231C)},
Pages = {920 - 3},
Address = {Arlington, VA, USA},
Year = {2006},
Keywords = {biomedical MRI;diagnostic radiography;haemorheology;image
resolution;lung;medical image processing;pneumodynamics;},
Abstract = {The high spatial and temporal resolution demands for imaging
physiological function in the rodent call for the use of
novel ways to combine information from different imaging
modalities. This work describes ventilation imaging using
hyperpolarized (HP) <sup>3</sup>He magnetic resonance
imaging (MRI) and perfusion imaging using X-ray digital
subtraction angiography (DSA). We illustrate the key steps
needed to combine the complementary data from the two
modalities to provide qualitative and quantitative
information on gas exchange in the lungs. The results
indicate that multimodality imaging of pulmonary function in
small animals can provide functional information at higher
spatial and temporal resolution compared to many traditional
imaging techniques},
Key = {9054396}
}
@booklet{Kamperschroer87,
Author = {J. H. Kamperschroer and H. W. Kugel and M. A. Reale and S.
L. Hayes and G. A. Johnson and J. L. Lowrance and P. A. Shah and P. Sichta and B. W. Sleaford and M. D. Williams and P.
M. Zucchino},
Title = {Multiple track doppler-shift spectroscopy system for tftr
neutral beam injectors},
Journal = {Review Of Scientific Instruments},
Volume = {58},
Number = {8},
Pages = {1362 -- 1368},
Year = {1987},
Month = {August},
Key = {Kamperschroer87}
}
@article{fds268799,
Author = {Johnson, G and Thomson, DJ and Wu, EX and Williams,
SCR},
Title = {Multiple-window spectrum estimation applied to in vivo NMR
spectroscopy},
Journal = {Journal of Magnetic Resonance - Series B},
Volume = {110},
Number = {2},
Pages = {138-149},
Publisher = {Elsevier BV},
Year = {1996},
ISSN = {1064-1866},
url = {http://dx.doi.org/10.1006/jmrb.1996.0023},
Abstract = {Multiple-window spectrum estimation (MWSE) is a method of
deriving frequency spectra from time series. A set of
apodizing windows is applied to the time data and each
windowed data set is Fourier transformed. The windows are
prolate spheroidal sequences. These form the orthonormal set
of functions that is maximally concentrated in both time and
frequency domains. An iterative algorithm is then applied to
the data set to find a leastsquares estimate of the power
spectrum. In addition, statistical tests may be applied to
determine the existence of periodic components at particular
frequencies, their amplitudes, phases, and positions. The
method is quantitative and makes no lineshape assumptions.
Computer simulations were used to compare MWSE performance
with that of conventional Fourier-transform processing with
quantification by curve fitting. Signal-to-noise ratio,
spectral resolution, linearity, and susceptibility to
artifacts were compared. MWSE gives similar signal-to-noise
ratio and spectral resolution to Fourier-transform data and
is linear over three orders of magnitude but is much more
robust with respect to artifacts. In particular, data
truncation introduces no baseline distortion, broad baseline
humps are removed automatically, and large solvent peaks may
be easily removed without affecting adjacent lines. No
separate phase correction is required. MWSE gives more
accurate quantitative spectra, particularly when the time
data are imperfect. The method is, therefore, particularly
appropriate for processing in vivo data. The utility of the
MWSE method is demonstrated on in vivo 'H, 31P, and 13C NMR
spectroscopy data. e 1996 Academic Press,
Inc.},
Doi = {10.1006/jmrb.1996.0023},
Key = {fds268799}
}
@article{fds268823,
Author = {Pandit, P and Qi, Y and Story, J and King, KF and Johnson,
GA},
Title = {Multishot PROPELLER for high-field preclinical
MRI.},
Journal = {Magn Reson Med},
Volume = {64},
Number = {1},
Pages = {47-53},
Year = {2010},
Month = {July},
ISSN = {1522-2594},
url = {http://www.ncbi.nlm.nih.gov/pubmed/20572138},
Keywords = {Animals • Disease Models, Animal • Female •
Image Processing, Computer-Assisted • Liver Neoplasms,
Experimental • Magnetic Resonance Imaging • Mice
• Mice, Inbred C57BL • Radiography, Abdominal
• diagnosis • methods*},
Abstract = {With the development of numerous mouse models of cancer,
there is a tremendous need for an appropriate imaging
technique to study the disease evolution. High-field
T(2)-weighted imaging using PROPELLER (Periodically Rotated
Overlapping ParallEL Lines with Enhanced Reconstruction) MRI
meets this need. The two-shot PROPELLER technique presented
here provides (a) high spatial resolution, (b) high contrast
resolution, and (c) rapid and noninvasive imaging, which
enables high-throughput, longitudinal studies in
free-breathing mice. Unique data collection and
reconstruction makes this method robust against motion
artifacts. The two-shot modification introduced here retains
more high-frequency information and provides higher
signal-to-noise ratio than conventional single-shot
PROPELLER, making this sequence feasible at high fields,
where signal loss is rapid. Results are shown in a liver
metastases model to demonstrate the utility of this
technique in one of the more challenging regions of the
mouse, which is the abdomen.},
Language = {eng},
Doi = {10.1002/mrm.22376},
Key = {fds268823}
}
@article{fds268874,
Author = {Bucholz, EK and Song, J and Johnson, GA and Hancu,
I},
Title = {Multispectral imaging with three-dimensional rosette
trajectories.},
Journal = {Magn Reson Med},
Volume = {59},
Number = {3},
Pages = {581-589},
Year = {2008},
Month = {March},
ISSN = {0740-3194},
url = {http://www.ncbi.nlm.nih.gov/pubmed/18306410},
Keywords = {Adipose Tissue • Algorithms • Artifacts •
Body Water • Brain Mapping • Computer Simulation
• Humans • Image Processing, Computer-Assisted*
• Imaging, Three-Dimensional* • Magnetic Resonance
Imaging • anatomy & histology •
methods*},
Abstract = {Two-dimensional intersecting k-space trajectories have
previously been demonstrated to allow fast multispectral
imaging. Repeated sampling of k-space points leads to
destructive interference of the signal coming from the
off-resonance spectral peaks; on-resonance data
reconstruction yields images of the on-resonance peak, with
some of the off-resonance energy being spread as noise in
the image. A shift of the k-space data by a given
off-resonance frequency brings a second frequency of
interest on resonance, allowing the reconstruction of a
second spectral peak from the same k-space data. Given the
higher signal-to-noise per unit time characteristic of a 3D
acquisition, we extended the concept of intersecting
trajectories to three dimensions. A 3D, rosette-like pulse
sequence was designed and implemented on a clinical 1.5T
scanner. An iterative density compensation function was
developed to weight the 3D intersecting trajectories before
Fourier transformation. Three volunteers were scanned using
this sequence and separate fat and water images were
reconstructed from the same imaging dataset.},
Doi = {10.1002/mrm.21551},
Key = {fds268874}
}
@booklet{Sinor02,
Author = {S. J. Sinor and M. M. Joyce and S. J. Yankey and A. M.
Assiri and K. Kodali and L. S. Hartt and M. Robison and G.
A. Johnson and T. L. Ott},
Title = {Mx, estrogen receptor (ER), and progesterone receptor (PR)
expression in ovine placentomal and interplacentomal
endometrium during days 25 to 120 of pregnancy.},
Journal = {Biology Of Reproduction},
Volume = {66},
Pages = {244 -- 244},
Year = {2002},
Key = {Sinor02}
}
@article{fds132909,
Author = {TM Yelbuz and KL Waldo and X Zhang and M Zdanowicz and J Parker and TL
Creazzo, GA Johnson and ML Kirby},
Title = {Myocardial volume and organization are changed by failure of
addition of secondary heart field myocardium to the cardiac
outflow tract.},
Journal = {Developmental dynamics : an official publication of the
American Association of Anatomists, United
States},
Volume = {228},
Number = {2},
Pages = {152-60},
Year = {2003},
Month = {October},
ISSN = {1058-8388},
Keywords = {Animals • Cell Count • Cell Division • Cell
Lineage • Cell Movement • Cell Size • Chick
Embryo • Heart • Heart Atria • Heart Defects,
Congenital • Heart Ventricles •
Immunohistochemistry • Magnetic Resonance Imaging
• Microscopy, Confocal • Myocardium •
Myocytes, Cardiac • Neural Crest • Time Factors
• cytology • cytology* • embryology •
etiology* • pathology • surgery*},
Abstract = {Cardiac neural crest ablation results in primary myocardial
dysfunction and failure of the secondary heart field to add
the definitive myocardium to the cardiac outflow tract. The
current study was undertaken to understand the changes in
myocardial characteristics in the heart tube, including
volume, proliferation, and cell size when the myocardium
from the secondary heart field fails to be added to the
primary heart tube. We used magnetic resonance and confocal
microscopy to determine that the volume of myocardium in the
looped heart was dramatically reduced and the compact layer
of myocardium was thinner after neural crest ablation,
especially in the outflow tract and ventricular regions.
Proliferation measured by 5-bromo-2'-deoxyuridine
incorporation was elevated at only one stage during looping,
cell death was normal and myocardial cell size was
increased. Taken together, these results indicate that there
are fewer myocytes in the heart. By incubation day 8 when
the heart would have normally completed septation, the
anterior (ventral) wall of the right ventricle and right
ventricular outflow tract was significantly thinner in the
neural crest-ablated embryos than normal, but the thickness
of the compact myocardium was normal in all other regions of
the heart. The decreased volume and number of myocardial
cells in the heart tube after neural crest ablation most
likely reflects the amount of myocardium added by the
secondary heart field.},
Key = {fds132909}
}
@booklet{Yelbuz03a,
Author = {Yelbuz, TM and Waldo, KL and Zhang, X and Zdanowicz, M and Parker, J and Creazzo, TL and Johnson, GA and Kirby, ML},
Title = {Myocardial volume and organization are changed by failure of
addition of secondary heart field myocardium to the cardiac
outflow tract.},
Journal = {Dev Dyn},
Volume = {228},
Number = {2},
Pages = {152-160},
Year = {2003},
Month = {October},
ISSN = {1058-8388},
url = {http://www.ncbi.nlm.nih.gov/pubmed/14517987},
Abstract = {Cardiac neural crest ablation results in primary myocardial
dysfunction and failure of the secondary heart field to add
the definitive myocardium to the cardiac outflow tract. The
current study was undertaken to understand the changes in
myocardial characteristics in the heart tube, including
volume, proliferation, and cell size when the myocardium
from the secondary heart field fails to be added to the
primary heart tube. We used magnetic resonance and confocal
microscopy to determine that the volume of myocardium in the
looped heart was dramatically reduced and the compact layer
of myocardium was thinner after neural crest ablation,
especially in the outflow tract and ventricular regions.
Proliferation measured by 5-bromo-2'-deoxyuridine
incorporation was elevated at only one stage during looping,
cell death was normal and myocardial cell size was
increased. Taken together, these results indicate that there
are fewer myocytes in the heart. By incubation day 8 when
the heart would have normally completed septation, the
anterior (ventral) wall of the right ventricle and right
ventricular outflow tract was significantly thinner in the
neural crest-ablated embryos than normal, but the thickness
of the compact myocardium was normal in all other regions of
the heart. The decreased volume and number of myocardial
cells in the heart tube after neural crest ablation most
likely reflects the amount of myocardium added by the
secondary heart field.},
Doi = {10.1002/dvdy.10364},
Key = {Yelbuz03a}
}
@article{fds357208,
Author = {Wang, N and Zhang, J and Cofer, G and Qi, Y and Anderson, RJ and White, LE and Allan Johnson and G},
Title = {Neurite orientation dispersion and density imaging of mouse
brain microstructure.},
Journal = {Brain Struct Funct},
Volume = {224},
Number = {5},
Pages = {1797-1813},
Year = {2019},
Month = {June},
url = {http://dx.doi.org/10.1007/s00429-019-01877-x},
Abstract = {Advanced biophysical models like neurite orientation
dispersion and density imaging (NODDI) have been developed
to estimate the microstructural complexity of voxels
enriched in dendrites and axons for both in vivo and ex vivo
studies. NODDI metrics derived from high spatial and angular
resolution diffusion MRI using the fixed mouse brain as a
reference template have not yet been reported due in part to
the extremely long scan time required. In this study, we
modified the three-dimensional diffusion-weighted spin-echo
pulse sequence for multi-shell and undersampling acquisition
to reduce the scan time. This allowed us to acquire several
exhaustive datasets that would otherwise not be attainable.
NODDI metrics were derived from a complex 8-shell diffusion
(1000-8000 s/mm2) dataset with 384 diffusion
gradient-encoding directions at 50 µm isotropic
resolution. These provided a foundation for exploration of
tradeoffs among acquisition parameters. A three-shell
acquisition strategy covering low, medium, and high b values
with at least angular resolution of 64 is essential for ex
vivo NODDI experiments. The good agreement between neurite
density index (NDI) and the orientation dispersion index
(ODI) with the subsequent histochemical analysis of myelin
and neuronal density highlights that NODDI could provide new
insight into the microstructure of the brain. Furthermore,
we found that NDI is sensitive to microstructural variations
in the corpus callosum using a well-established
demyelination cuprizone model. The study lays the ground
work for developing protocols for routine use of
high-resolution NODDI method in characterizing brain
microstructure in mouse models.},
Doi = {10.1007/s00429-019-01877-x},
Key = {fds357208}
}
@article{fds268868,
Author = {Badea, A and Nicholls, PJ and Johnson, GA and Wetsel,
WC},
Title = {Neuroanatomical phenotypes in the reeler
mouse.},
Journal = {Neuroimage},
Volume = {34},
Number = {4},
Pages = {1363-1374},
Year = {2007},
Month = {February},
ISSN = {1053-8119},
url = {http://www.ncbi.nlm.nih.gov/pubmed/17185001},
Keywords = {Animals • Brain • Female • Heterozygote
• Magnetic Resonance Imaging • Male • Mice
• Mice, Neurologic Mutants • Mutation •
Phenotype • anatomy & histology* •
genetics},
Abstract = {The reeler mouse (Reln) has been proposed as a
neurodevelopmental model for certain neurological and
psychiatric conditions and has been studied by qualitative
histochemistry and electron microscopy. Using magnetic
resonance microscopy (MRM), we have quantitated for the
first time the neuromorphology of Reln mice at a resolution
of 21.5 microm. The neuroanatomical phenotypes of
heterozygous and homozygous mutant Reln mice were compared
to those of wild type (WT) littermates using morphometry and
texture analysis. The cortical, hippocampal, and cerebellar
phenotypes of the heterozygous and homozygous mutant Reln
mice were confirmed, and new features were revealed. The
Reln(rl/rl) mice possessed a smaller brain, and both
Reln(rl/+) and Reln(rl/rl) mice had increased ventricles
compared to WT controls. Shape differences were found
between WT and Reln(rl/rl) brains, specifically in
cerebellum, olfactory bulbs, dorsomedial frontal and
parietal cortex, certain regions of temporal and occipital
lobes, as well as in the lateral ventricles and ventral
hippocampus. These findings suggest that certain brain
regions may be more severely impacted by the Reln mutation
than others. Gadolinium-based active staining demonstrated
that layers of the hippocampus were disorganized in
Reln(rl/rl) mice and differences in thickness of these
layers were identified between WT and Reln(rl/rl) mice. The
intensity distributions characteristic to the dorsal,
middle, and ventral hippocampus were altered in the
Reln(rl/rl), especially in the ventral hippocampus. These
differences were quantified using skewness and modeling the
intensity distributions with a Gaussian mixture. Our results
suggest that structural features of Reln(rl/rl) brain most
closely phenocopy those of patients with Norman-Roberts
lissencephaly.},
Doi = {10.1016/j.neuroimage.2006.09.053},
Key = {fds268868}
}
@booklet{Morgan04,
Author = {Morgan, DL and Little, PB and Herr, DW and Moser, VC and Collins, B and Herbert, R and Johnson, GA and Maronpot, RR and Harry, GJ and Sills,
RC},
Title = {Neurotoxicity of carbonyl sulfide in F344 rats following
inhalation exposure for up to 12 weeks.},
Journal = {Toxicol Appl Pharmacol},
Volume = {200},
Number = {2},
Pages = {131-145},
Year = {2004},
Month = {October},
ISSN = {0041-008X},
url = {http://www.ncbi.nlm.nih.gov/pubmed/15476866},
Abstract = {Carbonyl sulfide (COS), a high-priority Clean Air Act
chemical, was evaluated for neurotoxicity in short-term
studies. F344 rats were exposed to 75-600 ppm COS 6 h per
day, 5 days per week for up to 12 weeks. In rats exposed to
500 or 600 ppm for up to 4 days, malacia and microgliosis
were detected in numerous neuroanatomical regions of the
brain by conventional optical microscopy and magnetic
resonance microscopy (MRM). After a 2-week exposure to 400
ppm, rats were evaluated using a functional observational
battery. Slight gait abnormality was detected in 50% of the
rats and hypotonia was present in all rats exposed to COS.
Decreases in motor activity, and forelimb and hindlimb grip
strength were also detected. In rats exposed to 400 ppm for
12 weeks, predominant lesions were in the parietal cortex
area 1 (necrosis) and posterior colliculus (neuronal loss,
microgliosis, hemorrhage), and occasional necrosis was
present in the putamen, thalamus, and anterior olivary
nucleus. Carbonyl sulfide specifically targeted the auditory
system including the olivary nucleus, nucleus of the lateral
lemniscus, and posterior colliculus. Consistent with these
findings were alterations in the amplitude of the brainstem
auditory evoked responses (BAER) for peaks N3, P4, N4, and
N5 that represented changes in auditory transmission between
the anterior olivary nucleus to the medial geniculate
nucleus in animals after exposure for 2 weeks to 400 ppm
COS. A concentration-related decrease in cytochrome oxidase
activity was detected in the posterior colliculus and
parietal cortex of exposed rats as early as 3 weeks.
Cytochrome oxidase activity was significantly decreased at
COS concentrations that did not cause detectable lesions,
suggesting that disruption of the mitochondrial respiratory
chain may precede these brain lesions. Our studies
demonstrate that this environmental air contaminant has the
potential to cause a wide spectrum of brain lesions that are
dependent on the degree and duration of exposure.},
Doi = {10.1016/j.taap.2004.04.013},
Key = {Morgan04}
}
@article{fds132915,
Author = {DL Morgan and PB Little and DW Herr and VC Moser and B Collins and R
Herbert, GA Johnson and RR Maronpot and GJ Harry and RC
Sills},
Title = {Neurotoxicity of carbonyl sulfide in F344 rats following
inhalation exposure for up to 12 weeks.},
Journal = {Toxicology and applied pharmacology, United
States},
Volume = {200},
Number = {2},
Pages = {131-45},
Year = {2004},
Month = {October},
ISSN = {0041-008X},
Keywords = {Air Pollutants • Animals • Behavior, Animal •
Blood Chemical Analysis • Brain Diseases •
Electron Transport Complex IV • Evoked Potentials,
Auditory, Brain Stem • Female • Histocytochemistry
• Inhalation Exposure • Male • Motor Activity
• Neurotoxins • Random Allocation • Rats
• Rats, Inbred F344 • Sulfur Oxides •
analysis • chemically induced* • drug effects
• pathology • toxicity*},
Abstract = {Carbonyl sulfide (COS), a high-priority Clean Air Act
chemical, was evaluated for neurotoxicity in short-term
studies. F344 rats were exposed to 75-600 ppm COS 6 h per
day, 5 days per week for up to 12 weeks. In rats exposed to
500 or 600 ppm for up to 4 days, malacia and microgliosis
were detected in numerous neuroanatomical regions of the
brain by conventional optical microscopy and magnetic
resonance microscopy (MRM). After a 2-week exposure to 400
ppm, rats were evaluated using a functional observational
battery. Slight gait abnormality was detected in 50% of the
rats and hypotonia was present in all rats exposed to COS.
Decreases in motor activity, and forelimb and hindlimb grip
strength were also detected. In rats exposed to 400 ppm for
12 weeks, predominant lesions were in the parietal cortex
area 1 (necrosis) and posterior colliculus (neuronal loss,
microgliosis, hemorrhage), and occasional necrosis was
present in the putamen, thalamus, and anterior olivary
nucleus. Carbonyl sulfide specifically targeted the auditory
system including the olivary nucleus, nucleus of the lateral
lemniscus, and posterior colliculus. Consistent with these
findings were alterations in the amplitude of the brainstem
auditory evoked responses (BAER) for peaks N3, P4, N4, and
N5 that represented changes in auditory transmission between
the anterior olivary nucleus to the medial geniculate
nucleus in animals after exposure for 2 weeks to 400 ppm
COS. A concentration-related decrease in cytochrome oxidase
activity was detected in the posterior colliculus and
parietal cortex of exposed rats as early as 3 weeks.
Cytochrome oxidase activity was significantly decreased at
COS concentrations that did not cause detectable lesions,
suggesting that disruption of the mitochondrial respiratory
chain may precede these brain lesions. Our studies
demonstrate that this environmental air contaminant has the
potential to cause a wide spectrum of brain lesions that are
dependent on the degree and duration of exposure.},
Key = {fds132915}
}
@article{fds174295,
Author = {AY Grahn and KS Bankiewicz and M Dugich-Djordjevic, JR Bringas and P
Hadaczek, GA Johnson and S Eastman and M Luz},
Title = {Non-PEGylated liposomes for convection-enhanced delivery of
topotecan and gadodiamide in malignant glioma: initial
experience.},
Journal = {Journal of neuro-oncology},
Volume = {95},
Number = {2},
Pages = {185-97},
Year = {2009},
Month = {November},
ISSN = {1573-7373},
url = {http://dx.doi.org/10.1007/s11060-009-9917-1},
Keywords = {Animals • Antineoplastic Agents • Brain Neoplasms
• Cell Survival • Contrast Media • Convection
• Drug Delivery Systems* • Gadolinium DTPA •
Glioblastoma • Humans • Liposomes • Male
• Polyethylene Glycols • Rats • Rats,
Sprague-Dawley • Survival Rate • Tissue
Distribution • Topotecan • Tumor Cells, Cultured
• Xenograft Model Antitumor Assays •
administration & dosage • administration & dosage*
• chemistry* • drug effects • drug therapy*
• pathology • pharmacokinetics},
Abstract = {Convection-enhanced delivery (CED) of highly stable
PEGylated liposomes encapsulating chemotherapeutic drugs has
previously been effective against malignant glioma
xenografts. We have developed a novel, convectable
non-PEGylated liposomal formulation that can be used to
encapsulate both the topoisomerase I inhibitor topotecan
(topoCED) and paramagnetic gadodiamide (gadoCED), providing
an ideal basis for real-time monitoring of drug
distribution. Tissue retention of topoCED following single
CED administration was significantly improved relative to
free topotecan. At a dose of 10 microg (0.5 mg/ml), topoCED
had a half-life in brain of approximately 1 day and
increased the area under the concentration-time curve (AUC)
by 28-fold over free topotecan (153.8 vs. 5.5 microg day/g).
The combination of topoCED and gadoCED was found to
co-convect well in both naïve rat brain and malignant
glioma xenografts (correlation coefficients 0.97-0.99). In a
U87MG cell assay, the 50% inhibitory concentration (IC(50))
of topoCED was approximately 0.8 microM at 48 and 72 h; its
concentration-time curves were similar to free topotecan and
unaffected by gadoCED. In a U87MG intracranial rat xenograft
model, a two-dose CED regimen of topoCED co-infused with
gadoCED greatly increased median overall survival at dose
levels of 0.5 mg/ml (29.5 days) and 1.0 mg/ml (33.0 days)
vs. control (20.0 days; P < 0.0001 for both comparisons).
TopoCED at higher concentrations (1.6 mg/ml) co-infused with
gadoCED showed no evidence of histopathological changes
attributable to either agent. The positive results of tissue
pharmacokinetics, co-convection, cytotoxicity, efficacy, and
lack of toxicity of topoCED in a clinically meaningful dose
range, combined with an ideal matched-liposome paramagnetic
agent, gadoCED, implicates further clinical applications of
this therapy in the treatment of malignant
glioma.},
Language = {eng},
Doi = {10.1007/s11060-009-9917-1},
Key = {fds174295}
}
@article{fds268745,
Author = {MACFALL, JS and JOHNSON, GA},
Title = {NONDESTRUCTIVE, 3-DIMENSIONAL STUDY OF ROOT-GROWTH WITH
MAGNETIC-RESONANCE MICROSCOPY (MRM)},
Journal = {RESEARCH AND APPLICATIONS OF CHEMICAL SCIENCES IN
FORESTRY},
Volume = {104},
Pages = {18-18},
Publisher = {US DEPT AGR, FOREST SERV SO FOREST EXPT STN},
Editor = {Vozzo, JA},
Year = {1994},
Month = {January},
url = {http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:A1994BA75T00005&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=47d3190e77e5a3a53558812f597b0b92},
Key = {fds268745}
}
@article{fds174193,
Author = {FW Bazer and G Wu and TE Spencer and GA Johnson and RC Burghardt and K
Bayless},
Title = {Novel pathways for implantation and establishment and
maintenance of pregnancy in mammals.},
Journal = {Molecular human reproduction},
Volume = {16},
Number = {3},
Pages = {135-52},
Year = {2010},
Month = {March},
ISSN = {1460-2407},
url = {http://dx.doi.org/10.1093/molehr/gap095},
Keywords = {Animals • Embryo Implantation • Female •
Humans • Pregnancy • Pregnancy, Animal* •
Signal Transduction • Uterus • genetics •
metabolism • physiology • physiology*},
Abstract = {Uterine receptivity to implantation varies among species,
and involves changes in expression of genes that are
coordinate with attachment of trophectoderm to uterine
lumenal and superficial glandular epithelia, modification of
phenotype of uterine stromal cells, silencing of receptors
for progesterone and estrogen, suppression of genes for
immune recognition, alterations in membrane permeability to
enhance conceptus-maternal exchange of factors, angiogenesis
and vasculogenesis, increased vascularity of the
endometrium, activation of genes for transport of nutrients
into the uterine lumen, and enhanced signaling for pregnancy
recognition. Differential expression of genes by uterine
epithelial and stromal cells in response to progesterone,
glucocorticoids, prostaglandins and interferons may
influence uterine receptivity to implantation in mammals.
Uterine receptivity to implantation is progesterone-dependent;
however, implantation is preceded by loss of expression of
receptors for progesterone (PGR) so that progesterone most
likely acts via PGR-positive stromal cells throughout
pregnancy. Endogenous retroviruses expressed by the uterus
and/or blastocyst also affect implantation and placentation
in various species. Understanding the roles of the variety
of hormones, growth factors and endogenous retroviral
proteins in uterine receptivity for implantation is
essential to enhancing reproductive health and fertility in
humans and domestic animals.},
Language = {eng},
Doi = {10.1093/molehr/gap095},
Key = {fds174193}
}
@booklet{Johnson86b,
Author = {Johnson, GA and Thompson, MB and Gewalt, SL and Hayes,
CE},
Title = {Nuclear magnetic resonance imaging at microscopic
resolution},
Journal = {Journal of Magnetic Resonance (1969)},
Volume = {68},
Number = {1},
Pages = {129-137},
Publisher = {Elsevier BV},
Year = {1986},
ISSN = {0022-2364},
url = {http://dx.doi.org/10.1016/0022-2364(86)90322-7},
Abstract = {Resolution limits in NMR imaging are imposed by bandwidth
considerations, available magnetic gradients for spatial
encoding, and signal to noise. This work reports
modification of a clinical NMR imaging device with picture
elements of 500 × 500 × 5000 μm to yield picture elements
of 50 × 50 × 1000 μm. Resolution has been increased by
using smaller gradient coils permitting gradient fields
>0.4 mT/cm. Significant improvements in signal to noise
are achieved with smaller rf coils, close attention to
choice of bandwidth, and signal averaging. These
improvements permit visualization of anatomical structures
in the rat brain with an effective diameter of 1 cm with the
same definition as is seen in human imaging. The techniques
and instrumentation should open a number of basic sciences
such as embryology, plant sciences, and teratology to the
potentials of NMR imaging. © 1986.},
Doi = {10.1016/0022-2364(86)90322-7},
Key = {Johnson86b}
}
@article{fds132791,
Author = {JS MacFall and GA Johnson and PJ Kramer},
Title = {Observation of a water-depletion region surrounding loblolly
pine roots by magnetic resonance imaging.},
Journal = {Proceedings of the National Academy of Sciences of the
United States of America, United States},
Volume = {87},
Number = {3},
Pages = {1203-7},
Year = {1990},
Month = {February},
ISSN = {1091-6490},
Abstract = {Magnetic resonance imaging was used to study sand containing
various amounts of water and roots of loblolly pine planted
into similar sand. Spin-lattice (T1) relaxation times of
sand with water contents ranging from 0 to 25% (wt/wt)
ranged from 472 to 1265 ms and increased with water content.
Spin-spin (T2) relaxation times ranged from 54 to 76 ms and
did not change in a discernible pattern with water content.
Based on water content and measured T1 and T2 values, the
signal intensity of sand/water images was predicted to
increase with water content in a linear fashion, with the
slope of the lines increasing with the time of acquisition
repetition (TR). Measured signal intensity from images of
sand with various water contents was found to follow a
similar pattern. This allows interpretation of dark images
of sand/water to be regions of low water content, and bright
images to have comparatively greater water content. Images
of loblolly pine seedling roots planted in identical sand
showed the formation of a distinct water-depletion region
first around the woody taproot and later showed the region
extended and expanded around the lateral roots and clusters
of mycorrhizal short roots. This observation strongly
suggests that water uptake is occurring through the
suberized region of the woody taproot.},
Key = {fds132791}
}
@booklet{Macfall90,
Author = {MacFall, JS and Johnson, GA and Kramer, PJ},
Title = {Observation of a water-depletion region surrounding loblolly
pine roots by magnetic resonance imaging.},
Journal = {Proc Natl Acad Sci U S A},
Volume = {87},
Number = {3},
Pages = {1203-1207},
Year = {1990},
Month = {February},
url = {http://www.ncbi.nlm.nih.gov/pubmed/11607063},
Abstract = {Magnetic resonance imaging was used to study sand containing
various amounts of water and roots of loblolly pine planted
into similar sand. Spin-lattice (T1) relaxation times of
sand with water contents ranging from 0 to 25% (wt/wt)
ranged from 472 to 1265 ms and increased with water content.
Spin-spin (T2) relaxation times ranged from 54 to 76 ms and
did not change in a discernible pattern with water content.
Based on water content and measured T1 and T2 values, the
signal intensity of sand/water images was predicted to
increase with water content in a linear fashion, with the
slope of the lines increasing with the time of acquisition
repetition (TR). Measured signal intensity from images of
sand with various water contents was found to follow a
similar pattern. This allows interpretation of dark images
of sand/water to be regions of low water content, and bright
images to have comparatively greater water content. Images
of loblolly pine seedling roots planted in identical sand
showed the formation of a distinct water-depletion region
first around the woody taproot and later showed the region
extended and expanded around the lateral roots and clusters
of mycorrhizal short roots. This observation strongly
suggests that water uptake is occurring through the
suberized region of the woody taproot.},
Doi = {10.1073/pnas.87.3.1203},
Key = {Macfall90}
}
@article{fds157082,
Author = {JS Macfall and PE Pfeffer and DB Rolin, JR Macfall and GA
Johnson},
Title = {Observation of the Oxygen Diffusion Barrier in Soybean
(Glycine max) Nodules with Magnetic Resonance
Microscopy.},
Journal = {Plant physiology},
Volume = {100},
Number = {4},
Pages = {1691-1697},
Year = {1992},
Month = {December},
ISSN = {0032-0889},
Abstract = {The effects of selected gas perfusion treatments on the
spinlattice relaxation times (T(1)) of the soybean (Glycine
max) nodule cortex and inner nodule tissue were studied with
(1)H high resolution magnetic resonance microscopy. Three
gas treatments were used: (a) perfusion with O(2) followed
by N(2); (b) O(2) followed by O(2); and (c) air followed by
N(2). Soybean plants with intact attached nodules were
placed into the bore of a superconducting magnet and a
selected root with nodules was perfused with the gas of
interest. Magnetic resonance images were acquired with
repetition times from 50 to 3200 ms. The method of partial
saturation was used to calculate T(1) times on selected
regions of the image. Calculated images based on T(1) showed
longer T(1) values in the cortex than in the inner nodule
during all of the gas perfusions. When nodules were perfused
with O(2)-O(2), there was no significant change in the T(1)
of the nodule between the two gas treatments. When the
nodule was perfused with O(2)-N(2) or air-N(2), however, the
T(1) of both the cortex and inner nodule increased. In these
experiments, the increase in T(1) of the cortex was 2- to
3-fold greater than the increase observed in the inner
nodule. A similar change in T(1) was found in detached live
nodules, but there was no change in T(1) with selective gas
perfusion of detached dead nodules. These observations
suggest that cortical cells respond differently to selected
gas perfusion than the inner nodule, with the boundary of
T(1) change sharply delineated at the interface of the inner
nodule and the inner cortex.},
Key = {fds157082}
}
@booklet{Macfall92,
Author = {Macfall, JS and Pfeffer, PE and Rolin, DB and Macfall, JR and Johnson,
GA},
Title = {Observation of the Oxygen Diffusion Barrier in Soybean
(Glycine max) Nodules with Magnetic Resonance
Microscopy.},
Journal = {Plant Physiol},
Volume = {100},
Number = {4},
Pages = {1691-1697},
Year = {1992},
Month = {December},
ISSN = {0032-0889},
url = {http://www.ncbi.nlm.nih.gov/pubmed/16653185},
Abstract = {The effects of selected gas perfusion treatments on the
spinlattice relaxation times (T(1)) of the soybean (Glycine
max) nodule cortex and inner nodule tissue were studied with
(1)H high resolution magnetic resonance microscopy. Three
gas treatments were used: (a) perfusion with O(2) followed
by N(2); (b) O(2) followed by O(2); and (c) air followed by
N(2). Soybean plants with intact attached nodules were
placed into the bore of a superconducting magnet and a
selected root with nodules was perfused with the gas of
interest. Magnetic resonance images were acquired with
repetition times from 50 to 3200 ms. The method of partial
saturation was used to calculate T(1) times on selected
regions of the image. Calculated images based on T(1) showed
longer T(1) values in the cortex than in the inner nodule
during all of the gas perfusions. When nodules were perfused
with O(2)-O(2), there was no significant change in the T(1)
of the nodule between the two gas treatments. When the
nodule was perfused with O(2)-N(2) or air-N(2), however, the
T(1) of both the cortex and inner nodule increased. In these
experiments, the increase in T(1) of the cortex was 2- to
3-fold greater than the increase observed in the inner
nodule. A similar change in T(1) was found in detached live
nodules, but there was no change in T(1) with selective gas
perfusion of detached dead nodules. These observations
suggest that cortical cells respond differently to selected
gas perfusion than the inner nodule, with the boundary of
T(1) change sharply delineated at the interface of the inner
nodule and the inner cortex.},
Doi = {10.1104/pp.100.4.1691},
Key = {Macfall92}
}
@article{fds174121,
Author = {GA Johnson},
Title = {Obstetric nursing is for men, too.},
Journal = {The American journal of nursing},
Volume = {66},
Number = {12},
Pages = {2714-5},
Year = {1966},
Month = {December},
ISSN = {0002-936X},
Keywords = {Female • Humans • Nurses, Male* • Obstetrical
Nursing* • Pregnancy},
Language = {eng},
Key = {fds174121}
}
@article{fds174227,
Author = {GW Fuller and GA Johnson},
Title = {ON THE DIFFERENTIATION AND CLASSIFICATION OF WATER
BACTERIA.},
Journal = {The Journal of experimental medicine},
Volume = {4},
Number = {5-6},
Pages = {609-626},
Year = {1899},
Month = {September},
ISSN = {1540-9538},
Language = {ENG},
Key = {fds174227}
}
@article{fds174081,
Author = {GW Fuller and GA Johnson},
Title = {ON THE QUESTION OF STANDARD METHODS FOR THE DETERMINATION OF
THE NUMBERS OF BACTERIA IN WATERS.},
Journal = {Journal. Boston Society of Medical Sciences},
Volume = {4},
Number = {4},
Pages = {85-86},
Year = {1900},
Month = {January},
Language = {ENG},
Key = {fds174081}
}
@article{fds174134,
Author = {GW Fuller and GA Johnson},
Title = {On the Question of Standard Methods for the Determination of
the Numbers of Bacteria in Waters.},
Journal = {Public health papers and reports},
Volume = {25},
Pages = {574-9},
Year = {1899},
ISSN = {0737-8769},
Language = {eng},
Key = {fds174134}
}
@article{fds174279,
Author = {GW Fuller and GA Johnson},
Title = {On the Question of Standard Methods for the Determination of
the Numbers of Bacteria in Waters.},
Journal = {Public health papers and reports},
Volume = {25},
Pages = {574-579},
Year = {1899},
Language = {ENG},
Key = {fds174279}
}
@booklet{Shattuck95,
Author = {Shattuck, MD and Behringer, RP and Johnson, GA and Georgiadis,
JG},
Title = {Onset and Stability of Convection in Porous Media:
Visualization by Magnetic Resonance Imaging.},
Journal = {Phys Rev Lett},
Volume = {75},
Number = {10},
Pages = {1934-1937},
Year = {1995},
Month = {September},
url = {http://dx.doi.org/10.1103/PhysRevLett.75.1934},
Doi = {10.1103/PhysRevLett.75.1934},
Key = {Shattuck95}
}
@article{fds132859,
Author = {MD Shattuck and RP Behringer and GA Johnson and JG
Georgiadis},
Title = {Onset and Stability of Convection in Porous Media:
Visualization by Magnetic Resonance Imaging.},
Journal = {Phys Rev Lett},
Volume = {75},
Number = {10},
Pages = {1934-1937},
Year = {1995},
Month = {September},
ISSN = {0031-9007},
Key = {fds132859}
}
@article{fds268775,
Author = {Oldham, M and Sakhalkar, H and Oliver, T and Allan Johnson and G and Dewhirst, M},
Title = {Optical clearing of unsectioned specimens for
three-dimensional imaging via optical transmission and
emission tomography.},
Journal = {J Biomed Opt},
Volume = {13},
Number = {2},
Pages = {021113},
Year = {2008},
ISSN = {1083-3668},
url = {http://www.ncbi.nlm.nih.gov/pubmed/18465962},
Abstract = {Optical computed tomography (optical-CT) and optical
emission computed tomography (optical-ECT) are new
techniques that enable unprecedented high-resolution 3-D
multimodal imaging of tissue structure and function.
Applications include imaging macroscopic gene expression and
microvasculature structure in unsectioned biological
specimens up to 8 cm(3). A key requisite for these imaging
techniques is effective sample preparation including optical
clearing, which enables light transport through the sample
while preserving the signal (either light absorbing stain or
fluorescent proteins) in representative form. We review
recent developments in optical-CT and optical-ECT, and
compatible "fluorescence-friendly" optical clearing
protocols.},
Doi = {10.1117/1.2907968},
Key = {fds268775}
}
@booklet{Gupta02,
Author = {Gupta, AK and Johnson, GA and Nelson, RC},
Title = {Optimization of eight-element multi-detector helical CT for
imaging the abdomen},
Journal = {Radiology},
Volume = {222},
Number = {2},
Pages = {589-589},
Year = {2002},
Month = {February},
Key = {Gupta02}
}
@article{fds132823,
Author = {AK Gupta and RC Nelson and GA Johnson and EK Paulson and DM Delong and TT
Yoshizumi},
Title = {Optimization of eight-element multi-detector row helical CT
technology for evaluation of the abdomen.},
Journal = {Radiology, United States},
Volume = {227},
Number = {3},
Pages = {739-45},
Year = {2003},
Month = {June},
ISSN = {0033-8419},
Keywords = {Artifacts • Humans • Phantoms, Imaging •
Radiation Dosage • Radiography, Abdominal •
Tomography Scanners, X-Ray Computed • Tomography,
Spiral Computed* • methods • methods*},
Abstract = {PURPOSE: To evaluate protocols for abdominal imaging with an
eight-element multi-detector row computed tomographic (CT)
scanner. MATERIALS AND METHODS: An eight-element helical CT
scanner was used to acquire data in two phantoms with
four-element (pitch, 0.75 and 1.5; section thickness, 1.25,
2.5, and 5.0 mm) and eight-element (pitch, 0.625, 0.875,
1.35 and 1.675; section thickness, 1.25 and 2.5 mm)
protocols. One phantom was used for low-contrast
detectability and streak artifact; the other, for
high-contrast performance. Protocols included near constant
radiation dose (140 kV and varied tube current, confirmed by
using the above protocols to scan a dedicated radiation dose
phantom). Data were analyzed by three blinded readers for
streak artifacts, contrast-to-noise ratio, and z-axis
resolution (contrast-transfer function). Statistical
analysis included studentized range tests. RESULTS:
Contrast-to-noise ratios for four and eight elements were
not consistently different. Qualitative evaluation for
streak artifacts revealed fewer artifacts for all
eight-element 1.25-mm-thick section protocols, as compared
with eight-element 2.5-mm protocols. All eight-element
2.5-mm protocols except that with 27.0 mm per rotation had
fewer streak artifacts than did four-element protocols (P
=.02-.04). Contrast-transfer functions along the z axis for
eight-element protocols were better than those for
four-element protocols, demonstrating improved z-axis
resolution (P <.05). CONCLUSION: Images acquired at eight
sections per rotation demonstrated no sacrifice of
contrast-to-noise ratio, improved z-axis resolution, and
fewer streak artifacts, even when radiation dose was similar
to that for four-element CT.},
Key = {fds132823}
}
@booklet{Gupta03,
Author = {Gupta, AK and Nelson, RC and Johnson, GA and Paulson, EK and Delong, DM and Yoshizumi, TT},
Title = {Optimization of eight-element multi-detector row helical CT
technology for evaluation of the abdomen.},
Journal = {Radiology},
Volume = {227},
Number = {3},
Pages = {739-745},
Year = {2003},
Month = {June},
ISSN = {0033-8419},
url = {http://www.ncbi.nlm.nih.gov/pubmed/12702826},
Abstract = {PURPOSE: To evaluate protocols for abdominal imaging with an
eight-element multi-detector row computed tomographic (CT)
scanner. MATERIALS AND METHODS: An eight-element helical CT
scanner was used to acquire data in two phantoms with
four-element (pitch, 0.75 and 1.5; section thickness, 1.25,
2.5, and 5.0 mm) and eight-element (pitch, 0.625, 0.875,
1.35 and 1.675; section thickness, 1.25 and 2.5 mm)
protocols. One phantom was used for low-contrast
detectability and streak artifact; the other, for
high-contrast performance. Protocols included near constant
radiation dose (140 kV and varied tube current, confirmed by
using the above protocols to scan a dedicated radiation dose
phantom). Data were analyzed by three blinded readers for
streak artifacts, contrast-to-noise ratio, and z-axis
resolution (contrast-transfer function). Statistical
analysis included studentized range tests. RESULTS:
Contrast-to-noise ratios for four and eight elements were
not consistently different. Qualitative evaluation for
streak artifacts revealed fewer artifacts for all
eight-element 1.25-mm-thick section protocols, as compared
with eight-element 2.5-mm protocols. All eight-element
2.5-mm protocols except that with 27.0 mm per rotation had
fewer streak artifacts than did four-element protocols (P
=.02-.04). Contrast-transfer functions along the z axis for
eight-element protocols were better than those for
four-element protocols, demonstrating improved z-axis
resolution (P <.05). CONCLUSION: Images acquired at eight
sections per rotation demonstrated no sacrifice of
contrast-to-noise ratio, improved z-axis resolution, and
fewer streak artifacts, even when radiation dose was similar
to that for four-element CT.},
Doi = {10.1148/radiol.2273020591},
Key = {Gupta03}
}
@article{fds268867,
Author = {Jaffe, TA and Nelson, RC and Johnson, GA and Lee, ER and Yoshizumi, TT and Lowry, CR and Bullard, AB and DeLong, DM and Paulson,
EK},
Title = {Optimization of multiplanar reformations from isotropic data
sets acquired with 16-detector row helical CT
scanner.},
Journal = {Radiology},
Volume = {238},
Number = {1},
Pages = {292-299},
Year = {2006},
Month = {January},
ISSN = {0033-8419},
url = {http://www.ncbi.nlm.nih.gov/pubmed/16373774},
Keywords = {Adult • Aged • Equipment Design • Female
• Humans • Image Processing, Computer-Assisted*
• Male • Middle Aged • Phantoms, Imaging
• Radiation Dosage • Radiography, Abdominal •
Retrospective Studies • Tomography Scanners, X-Ray
Computed* • Tomography, Spiral Computed* •
instrumentation*},
Abstract = {Institutional review board approval and waiver of consent
were obtained for the patient component of this
retrospective HIPAA-compliant study. By using an
anthropomorphic phantom and metal oxide semiconductor field
effect transistor detectors, radiation dose was determined
for one eight-detector row and two 16-detector row computed
tomographic (CT) protocols. A custom phantom was scanned by
using the three protocols to identify isotropy.
Contrast-to-noise ratios (CNRs) were determined for the same
protocols by using a third phantom. Seven patients had
undergone isotropic 16-detector row CT of the abdomen and
pelvis. Anonymized coronal reformations at various
thicknesses were ranked qualitatively by three radiologists.
Effective dose equivalents were similar for the eight- and
16-detector row protocols. When transverse and coronal
reformations of data acquired in the custom phantom were
compared, coronal reformations obtained with the 16-detector
row and 0.625-mm section thickness protocol were found to be
nearly identical to the transverse image for all sets of
line pairs. CNRs were consistently highest on 5-mm-thick
coronal reformations (CNR range, 1.2-3.3). For qualitative
assessment, 2- and 3-mm-thick coronal reformations were
consistently preferred.},
Doi = {10.1148/radiol.2381050404},
Key = {fds268867}
}
@booklet{Hurlston97,
Author = {Hurlston, SE and Cofer, GP and Johnson, GA},
Title = {Optimized radiofrequency coils for increased signal-to-noise
ratio in magnetic resonance microscopy},
Journal = {International Journal of Imaging Systems and
Technology},
Volume = {8},
Number = {3},
Pages = {277-284},
Year = {1997},
Month = {January},
url = {http://dx.doi.org/10.1002/(SICI)1098-1098(1997)8:3<277::AID-IMA5>3.0.CO;2-7},
Abstract = {The signal-to-noise ratio (SNR) is a major obstacle to
achieving increased resolution in magnetic resonance
microscopy (MRM). The SNR considerations for MRM are
presented, with particular attention to the role of
judicious receiver coil design in maximizing sensitivity and
limiting noise contributions both from the sample and the
coil. We present a number of different coil configurations
that have been optimized for particular applications of MRM
in the biological sciences. An overview of the literature
regarding derivations of the SNR for birdcage-configuration
volume coils, inductively coupled surface coils, and
surgically implanted coils is presented in a unified
fashion. Microscopy coils designed to reduce the total
volume of excitation, thus coupling more closely to a given
region of interest, are discussed. The volume coil is
presented in terms of its application to lung imaging in
small animals at 2 T and imaging of stroke at 7 T. The
performance of traditional surface coils is demonstrated by
application to spinal cord imaging in the rat. Finally,
implanted coils are examined, as used in studies of the
carotid arteries. © 1997 John Wiley & Sons,
Inc.},
Doi = {10.1002/(SICI)1098-1098(1997)8:3<277::AID-IMA5>3.0.CO;2-7},
Key = {Hurlston97}
}
@article{fds268779,
Author = {De Lin and M and Badea, CT and Johnson, GA},
Title = {Optimized radiographic spectra for digital subtraction
angiography in the mouse},
Journal = {2004 2nd IEEE International Symposium on Biomedical Imaging:
Macro to Nano},
Volume = {2},
Pages = {1412-1415},
Year = {2004},
Month = {December},
ISBN = {9780780383883},
Abstract = {The availability of genetically altered mouse models of
human disease and the increasing use of small animals in
basic research have spurred extraordinary interest in new
imaging methodologies - particularly magnetic resonance
microscopy, microCT, and microPET. To date, very little
attention has been given to planar radiographic imaging. Yet
there exists enormous potential for this modality given the
ease of use and its potential speed. Functional imaging in
mouse models can be addressed particularly well through the
use of digital subtraction angiography. We describe here a
system designed explicitly for digital subtraction
angiography in the mouse and the optimization of acquisition
parameters required to perform the highest quality
functional subtraction angiograms. We focus on optimization
of contrast using selective K-edge filters and the
optimization of contrast agent though a carefully controlled
biological pulse sequence. © 2004 IEEE.},
Key = {fds268779}
}
@article{fds344782,
Author = {De Lin and M and Samei, E and Badea, CT and Yoshizumi, TT and Allan
Johnson, G},
Title = {Optimized radiographic spectra for small animal digital
subtraction angiography.},
Journal = {Med Phys},
Volume = {33},
Number = {11},
Pages = {4249-4257},
Year = {2006},
Month = {November},
ISSN = {0094-2405},
url = {http://dx.doi.org/10.1118/1.2356646},
Keywords = {Algorithms* • Angiography, Digital Subtraction •
Animals • Image Enhancement • Image
Interpretation, Computer-Assisted • Rats •
Reproducibility of Results • Sensitivity and
Specificity • Spectrometry, X-Ray Emission •
instrumentation • methods* • veterinary*},
Abstract = {The increasing use of small animals in basic research has
spurred interest in new imaging methodologies. Digital
subtraction angiography (DSA) offers a particularly
appealing approach to functional imaging in the small
animal. This study examines the optimal x-ray, molybdenum
(Mo) or tungsten (W) target sources, and technique to
produce the highest quality small animal functional
subtraction angiograms in terms of contrast and
signal-difference-to-noise ratio squared (SdNR2). Two
limiting conditions were considered-normalization with
respect to dose and normalization against tube loading.
Image contrast and SdNR2 were simulated using an established
x-ray model. DSA images of live rats were taken at two
representative tube potentials for the W and Mo sources.
Results show that for small animal DSA, the Mo source
provides better contrast. However, with digital detectors,
SdNR2 is the more relevant figure of merit. The W source
operated at kVps>60 achieved a higher SdNR2. The highest
SdNR2 was obtained at voltages above 90kVp. However,
operation at the higher potential results in significantly
greater dose and tube load and reduced contrast
quantization. A reasonable tradeoff can be achieved at tube
potentials at the beginning of the performance plateau,
around 70kVp, where the relative gain in SdNR2 is the
greatest.},
Doi = {10.1118/1.2356646},
Key = {fds344782}
}
@article{fds357204,
Author = {Anderson, RJ and Long, CM and Calabrese, ED and Robertson, SH and Johnson, GA and Cofer, GP and O'Brien, RJ and Badea,
A},
Title = {Optimizing Diffusion Imaging Protocols for Structural
Connectomics in Mouse Models of Neurological
Conditions.},
Journal = {Front Phys},
Volume = {8},
Year = {2020},
Month = {April},
url = {http://dx.doi.org/10.3389/fphy.2020.00088},
Abstract = {Network approaches provide sensitive biomarkers for
neurological conditions, such as Alzheimer's disease (AD).
Mouse models can help advance our understanding of
underlying pathologies, by dissecting vulnerable circuits.
While the mouse brain contains less white matter compared to
the human brain, axonal diameters compare relatively well
(e.g., ~0.6 μm in the mouse and ~0.65-1.05 μm in the human
corpus callosum). This makes the mouse an attractive test
bed for novel diffusion models and imaging protocols.
Remaining questions on the accuracy and uncertainty of
connectomes have prompted us to evaluate diffusion imaging
protocols with various spatial and angular resolutions. We
have derived structural connectomes by extracting gradient
subsets from a high-spatial, high-angular resolution
diffusion acquisition (120 directions, 43-μm-size voxels).
We have simulated protocols with 12, 15, 20, 30, 45, 60, 80,
100, and 120 angles and at 43, 86, or 172-μm voxel sizes.
The rotational stability of these schemes increased with
angular resolution. The minimum condition number was
achieved for 120 directions, followed by 60 and 45
directions. The percentage of voxels containing one dyad was
exceeded by those with two dyads after 45 directions, and
for the highest spatial resolution protocols. For the 86- or
172-μm resolutions, these ratios converged toward 55% for
one and 39% for two dyads, respectively, with <7% from
voxels with three dyads. Tractography errors, estimated
through dyad dispersion, decreased most with angular
resolution. Spatial resolution effects became noticeable at
172 μm. Smaller tracts, e.g., the fornix, were affected
more than larger ones, e.g., the fimbria. We observed an
inflection point for 45 directions, and an asymptotic
behavior after 60 directions, corresponding to similar
projection density maps. Spatially downsampling to 86 μm,
while maintaining the angular resolution, achieved a
subgraph similarity of 96% relative to the reference. Using
60 directions with 86- or 172-μm voxels resulted in 94%
similarity. Node similarity metrics indicated that major
white matter tracts were more robust to downsampling
relative to cortical regions. Our study provides guidelines
for new protocols in mouse models of neurological
conditions, so as to achieve similar connectomes, while
increasing efficiency.},
Doi = {10.3389/fphy.2020.00088},
Key = {fds357204}
}
@booklet{Johnson81b,
Author = {G. A. Johnson and J. J. Mcnamara},
Title = {Organ ischemia after hemorrhagic-shock},
Journal = {Surgical Forum},
Volume = {32},
Pages = {24 -- 26},
Year = {1981},
Key = {Johnson81b}
}
@article{fds174265,
Author = {WL Dees and JK Hiney and NH McArthur and GA Johnson and GA Dissen, SR
Ojeda},
Title = {Origin and ontogeny of mammalian ovarian
neurons.},
Journal = {Endocrinology},
Volume = {147},
Number = {8},
Pages = {3789-96},
Year = {2006},
Month = {August},
ISSN = {0013-7227},
url = {http://dx.doi.org/10.1210/en.2006-0394},
Keywords = {Age Factors • Aging • Animals • Cell Count
• Female • Ganglia, Sympathetic • Macaca
mulatta • Mammals • Neural Crest • Neurons
• Ovary • Receptor, Nerve Growth Factor •
Sexual Maturation • Swine • Tyrosine
3-Monooxygenase • cytology • cytology* •
embryology • growth & development* • innervation*
• metabolism • physiology},
Abstract = {Mammalian ovaries contain sympathetic neurons expressing the
low affinity neurotropin receptor (p75NTR). To date neither
the role these neurons might play in ovarian physiology nor
their embryological origin is known. Immunohistochemistry
was used to detect postnatal changes in distribution and
number of both p75NTR-positive and tyrosine
hydroxylase-positive neurons in rhesus monkey ovaries. Pig
fetuses were used to map the pathway of ovarian neuronal
migration during embryonic development. Antiserum to p75NTR
revealed the presence of isolated neurons and neurons
clustered into ganglia in 2-month-old monkey ovaries. After
8 months, the neurons exhibited well-developed processes,
and other than being more extensively interlaced, the
localization and morphology did not change after 2 yr of
age. Total number of p75NTR-positive neurons present
decreased gradually between 2 months and 12 yr of age and
declined markedly with reproductive aging. Conversely, the
subpopulation of neurons immunoreactive to anti-tyrosine
hydroxylase increased significantly at puberty and then
declined with the loss of reproductive capacity. By d 21 of
fetal life in the pig, p75NTR neurons had migrated medially
from the neural crest to form the paraaortic autonomic
ganglia. Some neurons migrated ventrally from the ganglia
and then continued ventrolaterally to enter the genital
ridge. By d 27, neurons had entered the developing ovary,
and by d 35, the migration was complete with neurons
demonstrating immunoreactivity to NeuN, a neuron-specific
marker. Results demonstrate that p75NTR-expressing ovarian
neurons originate from the neural crest and that a
catecholaminergic subset is associated with pubertal
maturation of the ovary and subsequent reproductive
function.},
Language = {eng},
Doi = {10.1210/en.2006-0394},
Key = {fds174265}
}
@article{fds174280,
Author = {MK Park and DH Lee and GA Johnson},
Title = {Oscillometric blood pressures in the arm, thigh, and calf in
healthy children and those with aortic coarctation.},
Journal = {Pediatrics},
Volume = {91},
Number = {4},
Pages = {761-5},
Year = {1993},
Month = {April},
ISSN = {0031-4005},
Keywords = {Adolescent • Aortic Coarctation • Arm •
Arteries • Blood Pressure Determination • Blood
Pressure* • Case-Control Studies • Child •
Child, Preschool • Female • Humans • Leg
• Male • Oscillometry • Reference Values
• Thigh • blood supply • diagnosis •
methods • physiology • physiopathology*},
Abstract = {Comparing blood pressure (BP) obtained in the arm with that
obtained in the thigh or calf is important in the diagnosis
of aortic coarctation. However, normative mean and range of
differences in BP between the arm and lower extremity sites
are not available for normal children. It is also not known
how accurately the differences in BP between the arm and the
lower extremity sites predict the pulsed Doppler estimation
of systolic pressure (SP) gradient across an aortic
coarctation. To resolve these questions, the authors
obtained two BP measurements by an oscillometric (Dinamap)
method in the arm, thigh, and calf in 74 healthy children
aged 4 to 16 years. Oscillometric BP was also obtained in 21
children aged 3 to 17 years with preoperative or
postoperative aortic coarctation and BP gradients were
compared with that estimated by the pulsed Doppler method.
Overall, SP was higher in the thigh and calf than in the
arm. The gradients in SP expressed as arm SP minus calf SP
[S(A-C)] and arm SP minus thigh SP [S(A-T)] were
significantly greater in children 4 through 8 years old than
in those 9 to 16 years old. The S(A-C) was -9.3 (+/- 7.4 SD)
mm Hg in the 4- through 8-year group and -5.0 (+/- 6.9 SD)
mm Hg in the 9- to 16-year group. The S(A-T) was -7.1 (+/-
6.8 SD) mm Hg in the 4- through 8-year group and -2.4 (+/-
7.7 SD) mm Hg in the 9- to 16-year group.(ABSTRACT TRUNCATED
AT 250 WORDS)},
Language = {eng},
Key = {fds174280}
}
@booklet{Park93,
Author = {M. K. Park and D. H. Lee and G. A. Johnson},
Title = {Oscillometric blood pressures in the arm, thigh, and calf in
healthy-children and those with aortic coarctation},
Journal = {Pediatrics},
Volume = {91},
Number = {4},
Pages = {761 -- 765},
Year = {1993},
Month = {April},
Key = {Park93}
}
@booklet{Lee92,
Author = {D. H. Lee and G. A. Johnson and M. K. Park},
Title = {Oscillometric blood-pressure in the arm, thigh and calf in
healthy-children and those with aortic coarctation},
Journal = {Clinical Research},
Volume = {40},
Number = {4},
Pages = {A834 -- A834},
Year = {1992},
Month = {December},
Key = {Lee92}
}
@booklet{Johnson01a,
Author = {G. A. Johnson and F. W. Bazer and J. E. Garlow and T. E.
Spencer and C. T. Pfarrer and R. C. Burghardt},
Title = {Osteopontin and activated alpha(v)beta(5) integrin receptors
during implantation and placentation in sheep.},
Journal = {Biology Of Reproduction},
Volume = {64},
Pages = {203 -- 203},
Year = {2001},
Key = {Johnson01a}
}
@booklet{Johnson03c,
Author = {G. A. Johnson and R. C. Burghardt and M. M. Joyce and T. E.
Spencer and F. W. Bazer and C. Pfarrer and C. A.
Gray},
Title = {Osteopontin expression in uterine stroma indicates a
decidualization-like differentiation during ovine
pregnancy},
Journal = {Biology Of Reproduction},
Volume = {68},
Number = {6},
Pages = {1951 -- 1958},
Year = {2003},
Month = {June},
Key = {Johnson03c}
}
@article{fds174239,
Author = {GA Johnson and RC Burghardt and MM Joyce and TE Spencer and FW Bazer and C
Pfarrer, CA Gray},
Title = {Osteopontin expression in uterine stroma indicates a
decidualization-like differentiation during ovine
pregnancy.},
Journal = {Biology of reproduction},
Volume = {68},
Number = {6},
Pages = {1951-8},
Year = {2003},
Month = {June},
ISSN = {0006-3363},
url = {http://dx.doi.org/10.1095/biolreprod.102.012948},
Keywords = {Actins • Animals • Cell Differentiation •
Decidua • Desmin • Embryo Implantation •
Endometrium • Female • Fluorescent Antibody
Technique • Genetic Markers • In Situ
Hybridization • Keratins • Muscle, Smooth •
Osteopontin • Pregnancy • RNA, Messenger •
Sheep • Sialoglycoproteins • Stromal Cells •
Swine • Uterus • Vimentin • biosynthesis
• biosynthesis* • cytology • metabolism
• metabolism* • physiology •
physiology*},
Abstract = {Osteopontin (OPN) is a component of the extracellular matrix
that interacts with cell surface receptors, including
integrins, to mediate cell adhesion, migration,
differentiation, survival, and immune function. In pregnant
mice and primates, OPN has been detected in decidualized
stroma and is considered to be a gene marker for
decidualization. Decidualization involves transformation of
spindle-like fibroblasts into polygonal epithelial-like
cells that are hypothesized to limit conceptus trophoblast
invasion through the uterine wall during invasive
implantation. Decidualization is not considered
characteristic of species with noninvasive implantation,
such as domestic animals. However, the extent of trophoblast
invasion between sheep and pigs differs, with sheep
exhibiting erosion of the uterine luminal epithelium (LE)
and fusion of trophectoderm with LE to form syncytia, and
pigs maintaining an intact LE throughout pregnancy.
Therefore, the present study measured changes in the
decidualization marker genes OPN, desmin, and alpha smooth
muscle actin (alphaSMA) in ovine and porcine uterine stroma
throughout pregnancy. The morphology of endometrial stromal
cells in pregnant ewes changes following conceptus
attachment, with cells increasing in size and becoming
polyhedral in shape by Day 35 of pregnancy. Expression of
OPN mRNA and protein, as well as desmin and alphaSMA
proteins, was observed in this same uterine stromal
compartment. In contrast, no morphological changes in
uterine stroma nor induction of OPN mRNA and protein, or
desmin protein, were detected during porcine pregnancy.
Interestingly, alphaSMA protein was absent on Day 20, but
prominent in uterine stroma of pregnant pigs on Day 45.
Collectively, these results indicate that the uterine stroma
of sheep undergoes a program of differentiation similar to
decidualization in invasive implanting species, whereas
porcine stroma exhibits differentiation that is more limited
than that in sheep, rodents, or primates. Results suggest
that uterine stromal decidualization is common to species
with different types of placentation, but the extent is
variable and correlates with the depth of trophoblast
invasion during implantation.},
Language = {eng},
Doi = {10.1095/biolreprod.102.012948},
Key = {fds174239}
}
@booklet{Johnson03b,
Author = {G. A. Johnson and R. C. Burghardt and M. M. Joyce and T. E.
Spencer and F. W. Bazer and C. A. Gray and C.
Pfarrer},
Title = {Osteopontin is synthesized by uterine glands and a 45-kDa
cleavage fragment is localized at the uterine-placental
interface throughout ovine pregnancy},
Journal = {Biology Of Reproduction},
Volume = {69},
Number = {1},
Pages = {92 -- 98},
Year = {2003},
Month = {July},
Key = {Johnson03b}
}
@article{fds174310,
Author = {GA Johnson and RC Burghardt and MM Joyce and TE Spencer and FW Bazer and CA
Gray, C Pfarrer},
Title = {Osteopontin is synthesized by uterine glands and a 45-kDa
cleavage fragment is localized at the uterine-placental
interface throughout ovine pregnancy.},
Journal = {Biology of reproduction},
Volume = {69},
Number = {1},
Pages = {92-8},
Year = {2003},
Month = {July},
ISSN = {0006-3363},
url = {http://dx.doi.org/10.1095/biolreprod.102.013573},
Keywords = {Animals • Endometrium • Extracellular Matrix
• Female • In Situ Hybridization • Molecular
Weight • Osteopontin • Peptide Fragments •
Placenta • Pregnancy • Pregnancy, Animal •
RNA, Messenger • Sheep • Sialoglycoproteins •
Uterus • biosynthesis* • chemistry • genetics
• metabolism • metabolism*},
Abstract = {Osteopontin (OPN) is a phosphorylated and glycosylated,
secreted protein that is present in various epithelial cells
and biological fluids. On freezing and thawing or treatment
with proteases, the native 70-kDa protein gives rise to 45-
and 24-kDa fragments. Secreted OPN functions as an
extracellular matrix (ECM) protein that binds cell surface
receptors to mediate cell-cell adhesion, cell-ECM
communication, and cell migration. In sheep and humans, OPN
is proposed to be a secretory product of uterine glandular
epithelium (GE) that binds to uterine luminal epithelium
(LE) and conceptus trophectoderm to mediate conceptus
attachment, which is essential to maintain pregnancy through
the peri-implantation period. Cell-cell adhesion,
communication, and migration likely are important at the
interface between uterus and placenta throughout pregnancy,
but to our knowledge, endometrial and/or placental
expression of OPN beyond the peri-implantation period has
not been documented in sheep. Therefore, the present study
determined temporal and spatial alterations in OPN mRNA and
protein expression in the ovine uterus between Days 25 and
120 of pregnancy. The OPN mRNA in total ovine endometrium
increased 30-fold between Days 40 and 80 of gestation. In
situ hybridization and immunofluorescence analyses revealed
that the predominant source of OPN mRNA and protein
throughout pregnancy was the uterine GE. Interestingly, the
45-kDa form of OPN was detected exclusively, continuously,
and abundantly along the apical surface of LE, on conceptus
trophectoderm, and along the uterine-placental interface of
both interplacentomal and placentomal regions through Day
120 of pregnancy. The 45-kDa OPN is a proteolytic cleavage
fragment of the native 70-kDa OPN, and it is the most
abundant form in uterine flushes during early pregnancy. The
45-kDa OPN is more stimulatory to cell attachment and cell
migration than the native 70-kDa protein. Collectively, the
present results support the hypothesis that ovine OPN is a
component of histotroph secreted by the uterine GE that
accumulates at the uterine-placental interface to influence
maternal-fetal interactions throughout gestation in
sheep.},
Language = {eng},
Doi = {10.1095/biolreprod.102.013573},
Key = {fds174310}
}
@booklet{Johnson02d,
Author = {G. A. Johnson and M. M. Joyce and R. C. Burghardt},
Title = {Osteopontin/early T-cell activation factor-1 is expressed by
fetal placental immune cells after day 20 of pregnancy in
sheep but not pigs.},
Journal = {Biology Of Reproduction},
Volume = {66},
Pages = {272 -- 273},
Year = {2002},
Key = {Johnson02d}
}
@booklet{Johnson03,
Author = {G. A. Johnson and R. C. Burghardt and F. W. Bazer and T. E.
Spencer},
Title = {Osteopontin: Roles in implantation and placentation},
Journal = {Biology Of Reproduction},
Volume = {69},
Number = {5},
Pages = {1458 -- 1471},
Year = {2003},
Month = {November},
Key = {Johnson03}
}
@article{fds174190,
Author = {GA Johnson and RC Burghardt and FW Bazer and TE Spencer},
Title = {Osteopontin: roles in implantation and placentation.},
Journal = {Biology of reproduction},
Volume = {69},
Number = {5},
Pages = {1458-71},
Year = {2003},
Month = {November},
ISSN = {0006-3363},
url = {http://dx.doi.org/10.1095/biolreprod.103.020651},
Keywords = {Animals • Decidua • Embryo Implantation •
Embryo, Mammalian • Endometrium • Female •
Humans • Immunity, Cellular • Integrins •
Osteopontin • Placenta • Placentation •
Pregnancy • Progesterone • Sialoglycoproteins
• Structure-Activity Relationship • Uterus •
biosynthesis • chemistry • immunology •
metabolism • physiology • physiology* •
secretion},
Abstract = {Osteopontin (OPN) is an acidic member of the small
integrin-binding ligand N-linked glycoprotein (SIBLING)
family of extracellular matrix proteins/cytokines that
undergoes extensive posttranslational modification,
including phosphorylation, glycosylation, and cleavage,
yielding molecular mass variants ranging in size from 25 to
75 kDa. The result is a versatile protein(s) with multiple
functions arising from its role as a mediator of cell-cell
and cell-extracellular matrix (ECM) communication that
encompass both normal and tumorigenic developmental
processes, immunological responses during inflammation and
wound healing, and biomineralization. Studies in primates,
pigs, sheep, and rodents have revealed that OPN is a major
constituent of the uterine-placental microenvironment with
influence as 1) a component of histotroph required for
adhesion and signal transduction at the uterine-placental
interface throughout pregnancy, 2) a gene product expressed
by uterine stroma contributing to a decidualization-like
transformation that correlates with the degree of conceptus
invasiveness, and 3) a product of resident uterine and
placental immune cells that may regulate their behavior and
cytokine production. This minireview summarizes information
regarding uterine and placental expression of OPN that has
accumulated over the past 15 yr, and we briefly describe
structural/functional properties of this protein that are
likely relevant to its role(s) during pregnancy. Comparative
studies have offered insights into the potential
hormonal/cytokine, cellular, and molecular mechanisms
underlying OPN-mediated adhesion, remodeling, and
cell-cell/cell-ECM communication within the uterus and
placenta. OPN has the potential to profoundly impact
pregnancy, and investigators are now challenged to focus on
the mechanistic nature of the functions of this multifaceted
and major component of the uterine-placental
microenvironment.},
Language = {eng},
Doi = {10.1095/biolreprod.103.020651},
Key = {fds174190}
}
@booklet{Shen-gunther03,
Author = {J. Shen-gunther and R. S. Mannel and J. L. Walker and M. A.
Gold and G. A. Johnson},
Title = {Outpatient implantation of a central venous access system in
gynecologic oncology patients},
Journal = {Journal Of Reproductive Medicine},
Volume = {48},
Number = {11},
Pages = {875 -- 881},
Year = {2003},
Month = {November},
Key = {Shen-gunther03}
}
@article{fds174236,
Author = {J Shen-Gunther and RS Mannel and JL Walker and MA Gold and GA
Johnson},
Title = {Outpatient implantation of a central venous access system in
gynecologic oncology patients.},
Journal = {The Journal of reproductive medicine},
Volume = {48},
Number = {11},
Pages = {875-81},
Year = {2003},
Month = {November},
ISSN = {0024-7758},
Keywords = {Adult • Aged • Aged, 80 and over • Ambulatory
Care* • Catheterization, Central Venous •
Catheters, Indwelling • Equipment Failure • Female
• Genital Neoplasms, Female • Humans • Middle
Aged • Oklahoma • Postoperative Complications
• Prospective Studies • Subclavian Vein •
drug therapy* • economics* • epidemiology •
radiography • surgery • utilization*},
Abstract = {OBJECTIVE: To determine the feasibility, accuracy,
complications and cost of implantation of the PORT-A-CATH II
Fluoro-Free venous access system (SIMS Deltec Inc., St.
Paul, Minnesota) in the procedure room setting. STUDY
DESIGN: A prospective study of 49 consecutive gynecologic
oncology patients who underwent 53 PORT-A-CATH II System
implantations was conducted. Local anesthesia and conscious
sedation were used for the procedure. To localize and
position the catheter tip, the CATH-FINDER (SIMS Deltec)
electronic catheter sensing device was utilized. Demographic
characteristics, operative data, complication rates, failure
rates and itemized costs were collected and analyzed.
RESULTS: For the 53 ports implanted, the mean operative time
was 54 minutes (range, 39-74) and mean estimated blood loss
was 17 mL (range, 7-50). Immediate complications included
failure to thread the catheter or guidewire past the left
subclavian vein (4 patients), pneumothorax (1) and
electronic wire fracture (1). All catheter tips were
positioned accurately, as confirmed by chest radiography.
The procedural charge ranged from $1,946 to $2,042. The
CATH-FINDER obviated the need for, and expenses of,
fluoroscopy, operating room and anesthesia services,
resulting in savings of approximately $2,000 per procedure.
CONCLUSION: Implantation of the PORT-A-CATH II System was
performed safely, accurately and cost effectively in the
procedure room setting. The advantages of functional
longevity, low complication rates and reduced cost of this
port system offer an excellent option for long-term central
venous access.},
Language = {eng},
Key = {fds174236}
}
@booklet{Johnson99b,
Author = {G. A. Johnson and T. E. Spencer and R. C. Burghardt and F.
W. Bazer},
Title = {Ovine osteopontin: I. Cloning and expression of messenger
ribonucleic acid in the uterus during the periimplantation
period},
Journal = {Biology Of Reproduction},
Volume = {61},
Number = {4},
Pages = {884 -- 891},
Year = {1999},
Month = {October},
Key = {Johnson99b}
}
@article{fds174150,
Author = {GA Johnson and TE Spencer and RC Burghardt and FW
Bazer},
Title = {Ovine osteopontin: I. Cloning and expression of messenger
ribonucleic acid in the uterus during the periimplantation
period.},
Journal = {Biology of reproduction},
Volume = {61},
Number = {4},
Pages = {884-91},
Year = {1999},
Month = {October},
ISSN = {0006-3363},
Keywords = {Amino Acid Sequence • Animals • Base Sequence
• Blotting, Western • Cloning, Molecular •
Embryonic Development • Estrus • Female •
Gene Expression Regulation, Developmental* • In Situ
Hybridization • Molecular Sequence Data •
Osteopontin • Pregnancy • Pregnancy, Animal •
RNA, Messenger • Sheep • Sialoglycoproteins •
Time Factors • Uterus • biosynthesis •
biosynthesis* • genetics* • metabolism* •
physiology • veterinary},
Abstract = {Trophoblast-derived interferon tau (IFNtau) acts on the
endometrium to increase secretion of several proteins during
the pregnancy recognition period in ruminants. One of these
is a 70-kDa acidic protein that has not been identified. Our
hypothesis was that the 70-kDa acidic protein is osteopontin
(OPN). OPN is an acidic glycoprotein that fragments upon
freezing and thawing or treatment with proteases including
thrombin. OPN contains a Gly-Arg-Gly-Asp-Ser (GRGDS)
sequence that binds to cell surface integrins to promote
cell-cell attachment and cell spreading. Using antisera to
recombinant human OPN, both 70-kDa and 45-kDa proteins were
identified in uterine flushings from pregnant ewes by
Western blotting. A clone containing the entire ovine OPN
cDNA coding sequence was isolated by screening a Day 15
pregnant ovine endometrial cDNA library with a partial ovine
OPN cDNA. In pregnant ewes, steady-state levels of OPN
endometrial mRNA increased (P < 0. 01) after Day 17. In both
cyclic and pregnant ewes, in situ hybridization analysis
showed that OPN mRNA was localized on unidentified immune
cells within the stratum compactum of the endometrium. In
pregnant ewes, OPN mRNA was also expressed by the glandular
epithelium. Results suggest that progesterone and/or IFNtau
induce expression and secretion of OPN by uterine glands
during the periimplantation period and that OPN may induce
adhesion between luminal epithelium and trophectoderm to
facilitate superficial implantation.},
Language = {eng},
Key = {fds174150}
}
@booklet{Johnson99c,
Author = {G. A. Johnson and R. C. Burghardt and T. E. Spencer and G.
C. R. Newton and T. L. Ott and F. W. Bazer},
Title = {Ovine osteopontin: II. Osteopontin and alpha(v)beta(3)
integrin expression in the uterus and conceptus during the
periimplantation period},
Journal = {Biology Of Reproduction},
Volume = {61},
Number = {4},
Pages = {892 -- 899},
Year = {1999},
Month = {October},
Key = {Johnson99c}
}
@article{fds174167,
Author = {GA Johnson and RC Burghardt and TE Spencer and GR Newton and TL Ott and FW
Bazer},
Title = {Ovine osteopontin: II. Osteopontin and alpha(v)beta(3)
integrin expression in the uterus and conceptus during the
periimplantation period.},
Journal = {Biology of reproduction},
Volume = {61},
Number = {4},
Pages = {892-9},
Year = {1999},
Month = {October},
ISSN = {0006-3363},
Keywords = {Animals • Blotting, Western • Culture Media,
Conditioned • Culture Techniques •
Electrophoresis, Polyacrylamide Gel • Embryo, Mammalian
• Embryonic Development* • Endometrium •
Female • Osteopontin • Pregnancy • Pregnancy,
Animal • Receptors, Vitronectin • Sheep •
Sialoglycoproteins • Time Factors • Uterus •
biosynthesis* • metabolism • metabolism* •
veterinary},
Abstract = {Osteopontin (OPN) is an acidic 70-kDa glycoprotein that is
cleaved by proteases to yield 45-kDa and 24-kDa fragments.
The 70-kDa and 45-kDa proteins contain a Gly-Arg-Gly-Asp-Ser
(GRGDS) sequence that binds to cell surface integrins
(primarily alpha(v)beta(3) heterodimer) to promote cell-cell
attachment and cell spreading. A 70-kDa acidic protein was
previously detected by two-dimensional (2D) PAGE in Day 17
pregnant endometrial cytosolic extracts using Stainsall and
identified as immunoreactive OPN using Western blotting.
Three forms of immunoreactive OPN proteins (70, 45, and 24
kDa) were detected by 1D PAGE and Western blot analysis of
endometrial extracts. OPN protein in endometrial extracts
did not differ between cyclic and pregnant ewes. However,
the amount of 45-kDa OPN increased in uterine flushings from
pregnant ewes between Days 11 and 17. Immunoreactive OPN was
localized to luminal and glandular epithelia of both cyclic
and pregnant ewes, and to trophectoderm of Day 19
conceptuses. The alpha(v) and beta(3) integrins were
detected on Day 19 endometrium and conceptuses by
immunofluorescence. It was reported that OPN mRNA increases
in the uterine glands of pregnant ewes and secretion of OPN
protein into the uterine lumen increases during early
pregnancy. The present results demonstrate accumulation of
OPN protein on endometrial LE and conceptus trophectoderm.
Therefore, it is hypothesized that progesterone and/or
interferon-tau induce expression, secretion and/or
proteolytic cleavage of OPN by uterine epithelium. Secreted
OPN is then available as ligand for alpha(v)beta(3) integrin
heterodimer on trophectoderm and uterus to 1) stimulate
changes in morphology of conceptus trophectoderm and 2)
induce adhesion between luminal epithelium and trophectoderm
essential for implantation and placentation.},
Language = {eng},
Key = {fds174167}
}
@article{fds174188,
Author = {S Noel and A Herman and GA Johnson and CA Gray, MD Stewart and FW Bazer and A Gertler and TE Spencer},
Title = {Ovine placental lactogen specifically binds to endometrial
glands of the ovine uterus.},
Journal = {Biology of reproduction},
Volume = {68},
Number = {3},
Pages = {772-80},
Year = {2003},
Month = {March},
ISSN = {0006-3363},
Keywords = {Animals • Binding Sites • Binding, Competitive
• Blotting, Western • Endometrium • Female
• Growth Hormone • Interferon Type I • Male
• Placental Hormones • Placental Lactogen •
Pregnancy Proteins • Prolactin • RNA • Random
Allocation • Receptors, Prolactin • Receptors,
Somatotropin • Reverse Transcriptase Polymerase Chain
Reaction • Sheep • chemistry • genetics
• metabolism • metabolism* • pharmacology
• physiology • ultrastructure •
veterinary},
Abstract = {A hormonal servomechanism has been proposed to regulate
differentiation and function of the endometrial glandular
epithelium (GE) in the ovine uterus during pregnancy. This
mechanism involves sequential actions of estrogen,
progesterone, ovine interferon tau (IFNtau), placental
lactogen (oPL), and placental growth hormone (oGH). The
biological actions of oPL in vitro are mediated by
homodimerization of the prolactin receptor (oPRLR) and
heterodimerization of the oPRLR and oGH receptor. The
objectives of the study were to determine the effects of
intrauterine oPL, oGH, and their combination on endometrial
histoarchitecture and gene expression and to localize and
characterize binding sites for oPL in the ovine uterus in
vivo using an in situ ligand binding assay. Intrauterine
infusion of oPL and/or oGH following IFNtau into
ovariectomized ewes treated with progesterone daily
differentially affected endometrial gland number and
expression of uterine milk proteins and osteopontin.
However, neither hormone affected PRLR, insulin-like growth
factor (IGF)-I, or IGF-II mRNA levels in the endometrium. A
chimeric protein of placental secretory alkaline phosphatase
(SEAP) and oPL was used to identify and characterize binding
sites for oPL in frozen sections of interplacentomal
endometrium from pregnant ewes. Specific binding of SEAP-oPL
was detected in the endometrial GE on Days 30, 60, 90, and
120 of pregnancy. In Day 90 endometrium, SEAP-oPL binding to
the endometrial GE was displaced completely by oPL and
prolactin (oPRL) but only partially by oGH. Binding
experiments using the extracellular domain of the oPRLR also
showed that iodinated oPL binding sites could be competed
for by oPRL and oPL but not by oGH. Collectively, results
indicate that oPL binds to receptors in the endometrial
glands and that oPRL is more effective than oGH in competing
for these binding sites. Thus, effects of oPL on the
endometrial glands may be mediated by receptors for oPRL and
oGH.},
Language = {eng},
Key = {fds174188}
}
@booklet{Noel03,
Author = {S. Noel and A. Herman and G. A. Johnson and C. A. Gray and M. D. Stewart and F. W. Bazer and A. Gertler and T. E.
Spencer},
Title = {Ovine placental lactogen specifically rinds to endometrial
glands of the ovine uterus},
Journal = {Biology Of Reproduction},
Volume = {68},
Number = {3},
Pages = {772 -- 780},
Year = {2003},
Month = {March},
Key = {Noel03}
}
@booklet{Carroll99,
Author = {T. L. Carroll and G. A. Johnson and L. M. Pecora and D. J.
Mar},
Title = {Parameter-insensitive and narrow-band synchronization of
chaotic circuits},
Journal = {International Journal Of Bifurcation And
Chaos},
Volume = {9},
Number = {11},
Pages = {2189 -- 2196},
Year = {1999},
Month = {November},
Key = {Carroll99}
}
@booklet{Schuster96,
Author = {H. G. Schuster and E. Niebur and E. R. Hunt and G. A.
Johnson and M. Locher},
Title = {Parametric feedback resonance in chaotic
systems},
Journal = {Physical Review Letters},
Volume = {76},
Number = {3},
Pages = {400 -- 403},
Year = {1996},
Month = {January},
Key = {Schuster96}
}
@article{fds132913,
Author = {HG Schuster and E Niebur and ER Hunt and GA Johnson and M
Löcher},
Title = {Parametric feedback resonance in chaotic
systems.},
Journal = {Physical review letters},
Volume = {76},
Number = {3},
Pages = {400-403},
Year = {1996},
Month = {January},
ISSN = {1079-7114},
Language = {ENG},
Key = {fds132913}
}
@booklet{Johnson96b,
Author = {G. A. Johnson and D. A. Mortensen and L. J. Young and A. R.
Martin},
Title = {Parametric sequential sampling based on multistage
estimation of the negative binomial parameter
k},
Journal = {Weed Science},
Volume = {44},
Number = {3},
Pages = {555 -- 559},
Year = {1996},
Key = {Johnson96b}
}
@article{fds132856,
Author = {BP Drayer and W Olanow and P Burger and GA Johnson and R Herfkens and S
Riederer},
Title = {Parkinson plus syndrome: diagnosis using high field MR
imaging of brain iron.},
Journal = {Radiology, UNITED STATES},
Volume = {159},
Number = {2},
Pages = {493-8},
Year = {1986},
Month = {May},
ISSN = {0033-8419},
Keywords = {Adult • Aged • Atrophy • Cerebellar Nuclei
• Globus Pallidus • Humans • Iron •
Magnetic Resonance Spectroscopy • Middle Aged •
Parkinson Disease • Putamen • Substantia Nigra
• analysis • analysis* • diagnosis* •
diagnostic use* • pathology},
Abstract = {The distribution of iron in the brain was analyzed using
high field strength (1.5 T) magnetic resonance (MR) imaging
in 14 healthy control individuals and six patients with
Parkinson plus syndromes (multisystem atrophy and
progressive supranuclear palsy) who were unresponsive to
antiparkinsonian therapy. The normal topographic
distribution of iron in the brain as indicated by high field
MR images coincided precisely with the distribution of iron
in the brain as determined by Perls staining for ferric
iron. In Parkinson plus syndromes, there were abnormally
increased concentrations of iron (decreased T2 relaxation
times) in the putamen, and less prominent increases in the
caudate nucleus and lateral pars compacta of the substantia
nigra. In high field strength MR images of normal patients,
the decreased signal intensity in the globus pallidus is
more prominent than that of the putamen. In MR images of
patients with Parkinson plus syndromes, the decreased signal
intensity of the putamen is more prominent than that of the
globus pallidus.},
Key = {fds132856}
}
@booklet{Drayer86c,
Author = {Drayer, BP and Olanow, W and Burger, P and Johnson, GA and Herfkens, R and Riederer, S},
Title = {Parkinson plus syndrome: diagnosis using high field MR
imaging of brain iron.},
Journal = {Radiology},
Volume = {159},
Number = {2},
Pages = {493-498},
Year = {1986},
Month = {May},
ISSN = {0033-8419},
url = {http://www.ncbi.nlm.nih.gov/pubmed/3961182},
Abstract = {The distribution of iron in the brain was analyzed using
high field strength (1.5 T) magnetic resonance (MR) imaging
in 14 healthy control individuals and six patients with
Parkinson plus syndromes (multisystem atrophy and
progressive supranuclear palsy) who were unresponsive to
antiparkinsonian therapy. The normal topographic
distribution of iron in the brain as indicated by high field
MR images coincided precisely with the distribution of iron
in the brain as determined by Perls staining for ferric
iron. In Parkinson plus syndromes, there were abnormally
increased concentrations of iron (decreased T2 relaxation
times) in the putamen, and less prominent increases in the
caudate nucleus and lateral pars compacta of the substantia
nigra. In high field strength MR images of normal patients,
the decreased signal intensity in the globus pallidus is
more prominent than that of the putamen. In MR images of
patients with Parkinson plus syndromes, the decreased signal
intensity of the putamen is more prominent than that of the
globus pallidus.},
Doi = {10.1148/radiology.159.2.3961182},
Key = {Drayer86c}
}
@booklet{Johnson84b,
Author = {G. A. Johnson},
Title = {Partitioned matrices satisfying certain null space
properties},
Journal = {Linear Algebra And Its Applications},
Volume = {57},
Number = {FEB},
Pages = {75 -- 85},
Year = {1984},
Key = {Johnson84b}
}
@booklet{Todd99,
Author = {M. D. Todd and G. A. Johnson and C. C. Chang},
Title = {Passive, light intensity-independent interferometric method
for fibre Bragg grating interrogation},
Journal = {Electronics Letters},
Volume = {35},
Number = {22},
Pages = {1970 -- 1971},
Year = {1999},
Month = {October},
Key = {Todd99}
}
@booklet{Baxter89,
Author = {Baxter, GW and Behringer, RP and Fagert, T and Johnson,
GA},
Title = {Pattern formation in flowing sand.},
Journal = {Phys Rev Lett},
Volume = {62},
Number = {24},
Pages = {2825-2828},
Year = {1989},
Month = {June},
url = {http://www.ncbi.nlm.nih.gov/pubmed/10040101},
Doi = {10.1103/PhysRevLett.62.2825},
Key = {Baxter89}
}
@article{fds132809,
Author = {GW Baxter and RP Behringer and T Fagert and GA Johnson},
Title = {Pattern formation in flowing sand.},
Journal = {Phys Rev Lett},
Volume = {62},
Number = {24},
Pages = {2825-2828},
Year = {1989},
Month = {June},
ISSN = {0031-9007},
Key = {fds132809}
}
@booklet{Miller99,
Author = {J. R. Miller and S. E. Hurlston and Q. Y. Ma and D. W. Face and D. J. Kountz and J. R. Macfall and L. W. Hedlund and G.
A. Johnson},
Title = {Performance of a high-temperature superconducting probe for
in vivo microscopy at 2.0 T},
Journal = {Magnetic Resonance In Medicine},
Volume = {41},
Number = {1},
Pages = {72 -- 79},
Year = {1999},
Month = {January},
Key = {Miller99}
}
@article{fds268967,
Author = {Miller, JR and Hurlston, SE and Ma, QY and Face, DW and Kountz, DJ and MacFall, JR and Hedlund, LW and Johnson, GA},
Title = {Performance of a high-temperature superconducting probe for
in vivo microscopy at 2.0 T.},
Journal = {Magn Reson Med},
Volume = {41},
Number = {1},
Pages = {72-79},
Year = {1999},
Month = {January},
ISSN = {0740-3194},
url = {http://www.ncbi.nlm.nih.gov/pubmed/10025613},
Keywords = {Animals • Artifacts • Brain • Female •
Heat* • Image Processing, Computer-Assisted •
Magnetic Resonance Imaging • Microscopy • Models,
Theoretical • Rats • Rats, Inbred F344 •
Thermal Conductivity • anatomy & histology •
methods* • physiology*},
Abstract = {The use of a high-temperature superconducting probe for in
vivo magnetic resonance microscopy at 2 T is described. To
evaluate the performance of the probe, a series of SNR
comparisons are carried out. The SNR increased by a factor
of 3.7 compared with an equivalent copper coil. Quantitative
measures of the SNR gain are in good agreement with
theoretical predictions. A number of issues that are unique
to the application of HTS coils are examined, including the
difficulty in obtaining homogenous excitation without
degrading the SNR of the probe. The use of the HTS probe in
transmit-receive mode is simple to implement but results in
nonuniform excitation. The effect of using the probe in this
mode of operation on the T1 and T2 contrast is investigated.
Methods for improving homogeneity are explored, such as
employing a transmit volume coil. It is found that the cost
of using an external transmit coil is an increased probe
noise temperature and a reduced SNR by approximately 30%.
Other important aspects of the probe are considered,
including the effect of temperature on probe stability.
Three-dimensional in vivo imaging sets are acquired to
assess the stability of the probe for long scans.
High-resolution images of the rat brain demonstrate the
utility of the probe for microscopy applications.},
Doi = {10.1002/(sici)1522-2594(199901)41:1<72::aid-mrm11>3.0.co;2-a},
Key = {fds268967}
}
@booklet{Black95,
Author = {Black, RD and Early, TA and Johnson, GA},
Title = {Performance of a High-Temperature Superconducting Resonator
for High-Field Imaging},
Journal = {Journal of Magnetic Resonance, Series A},
Volume = {113},
Number = {1},
Pages = {74-80},
Publisher = {Elsevier BV},
Year = {1995},
Month = {January},
ISSN = {1064-1858},
url = {http://dx.doi.org/10.1006/jmra.1995.1058},
Abstract = {The practicalities involved with the use of a
superconducting microimaging probe are outlined so that the
power and problems associated with this technology can be
assessed. The nonlinearity of the transmission
characteristics of this class of probe, the intrinsic limits
on bandwidth, the long ring-down times, the potential for
spin damping, and the difficulties of suppressing Johnson
noise are all discussed. Recent refinements that have
delivered a factor of 30 gain in SNR (signal-to-noise ratio)
relative to copper coils at room temperature are presented.
Further reductions in noise sources should yield a gain of a
factor of 60 in SNR. © 1995 Academic Press,
Inc.},
Doi = {10.1006/jmra.1995.1058},
Key = {Black95}
}
@booklet{Suddarth87,
Author = {Suddarth, SA and Johnson, GA and Sherrier, RH and Ravin,
CE},
Title = {Performance of high-resolution monitors for digital chest
imaging.},
Journal = {Med Phys},
Volume = {14},
Number = {2},
Pages = {253-257},
Year = {1987},
ISSN = {0094-2405},
url = {http://www.ncbi.nlm.nih.gov/pubmed/3587149},
Abstract = {High-resolution cathode-ray tubes (CRT's) are currently the
most viable soft-copy display for digital radiography. We
present here methods for measuring large-area contrast ratio
and detail contrast ratio. A two-dimensional charge coupled
device (ccd) array signal-averaged with a video frame buffer
permits linear microradiometric measure of individual beam
lines. Results from three different 1000-line monitors
demonstrate the shift variance of resolution. The detail
contrast ratio (or modulation depth) was found to vary from
100% to less than 10% across the face of one CRT. Dynamic
focus in both the horizontal and vertical deflection
circuitry proved effective in reducing this shift variance.
Comparisons of three phosphors demonstrate the utility of
long persistence phosphors (P164) for static display in
producing brighter images with less flicker. Recommendations
for CRT design and selection for high-resolution digital
radiography are included.},
Doi = {10.1118/1.596079},
Key = {Suddarth87}
}
@article{fds132798,
Author = {SA Suddarth and GA Johnson and RH Sherrier and CE
Ravin},
Title = {Performance of high-resolution monitors for digital chest
imaging.},
Journal = {Medical physics, UNITED STATES},
Volume = {14},
Number = {2},
Pages = {253-7},
ISSN = {0094-2405},
Keywords = {Computer Systems* • Humans • Radiographic Image
Enhancement • Radiography, Thoracic •
instrumentation*},
Abstract = {High-resolution cathode-ray tubes (CRT's) are currently the
most viable soft-copy display for digital radiography. We
present here methods for measuring large-area contrast ratio
and detail contrast ratio. A two-dimensional charge coupled
device (ccd) array signal-averaged with a video frame buffer
permits linear microradiometric measure of individual beam
lines. Results from three different 1000-line monitors
demonstrate the shift variance of resolution. The detail
contrast ratio (or modulation depth) was found to vary from
100% to less than 10% across the face of one CRT. Dynamic
focus in both the horizontal and vertical deflection
circuitry proved effective in reducing this shift variance.
Comparisons of three phosphors demonstrate the utility of
long persistence phosphors (P164) for static display in
producing brighter images with less flicker. Recommendations
for CRT design and selection for high-resolution digital
radiography are included.},
Key = {fds132798}
}
@article{fds204245,
Author = {JD Tobias and GA Johnson and M Patel},
Title = {Performance of the On-Q pain infusion device during
hyperbaric therapy.},
Journal = {Anesthesia and analgesia},
Volume = {113},
Number = {2},
Pages = {275-7},
Year = {2011},
Month = {August},
ISSN = {1526-7598},
url = {http://dx.doi.org/10.1213/ANE.0b013e31821c4035},
Keywords = {Air Pressure • Amides • Anesthesia, Conduction
• Anesthetics, Local • Data Display • Diving
• Fluid Therapy • Hyperbaric Oxygenation •
Infusion Pumps* • Pain, Postoperative •
Prospective Studies • administration & dosage •
drug therapy • instrumentation • methods* •
physiology},
Abstract = {BACKGROUND: There are reports in the literature regarding
the effects of hyperbaric conditions on various medical
devices. In the current study we evaluated the performance
of an elastomeric infusion device during exposure to a
hyperbaric environment. METHODS: Nineteen disposable 400-mL
On-Q pain ball infusion devices were filled with 0.2%
ropivacaine and connected to an infusion catheter. The
regulator of the device was set to deliver 14 mL/h.
Hyperbaric oxygen therapy included 7 minutes to achieve the
desired hyperbaric pressure level, 90 minutes at the
specific pressure (atm), and 7 minutes to return to normal
atmospheric pressure (1 atm), thereby resulting in a study
interval or dive of 104 minutes. The trials were performed
for the devices in the following sequence of dives with a
return to 1 atm between: 1, 2, 2.4, 2.8, 3, and 1 atm. The
fluid delivered during each dive was measured with a
graduated column. Additionally, the collection device was
weighed before and at the completion of each dive to
determine the change in weight as a measure of the total
amount of fluid infused. The output over 104 minutes was
also studied in 5 infusion devices without hyperbaric
pressure (control group). RESULTS: No difference in output
of the devices was noted when comparing the study group and
the control group. Although there was a decrease in the
output of the devices over 8 to 9 hours, no difference
between the 2 groups was noted. CONCLUSIONS: This
preliminary investigation demonstrates no clinically
significant change in the function of the On-Q pain device
during exposure to a hyperbaric environment.},
Language = {eng},
Doi = {10.1213/ANE.0b013e31821c4035},
Key = {fds204245}
}
@article{fds174183,
Author = {GA JOHNSON and RH MCCLUER},
Title = {PERIODATE OXIDATION STUDIES OF HUMAN BRAIN
GANGLIOSIDES.},
Journal = {Biochimica et biophysica acta},
Volume = {84},
Pages = {587-95},
Year = {1964},
Month = {October},
ISSN = {0006-3002},
Keywords = {CHROMATOGRAPHY* • GALACTOSE* • GANGLIOSIDES*
• GLUCOSE* • NEURAMINIC ACIDS* •
OXIDATION-REDUCTION* • PERIODIC ACIDS*},
Language = {eng},
Key = {fds174183}
}
@booklet{Fichter03,
Author = {C. R. Fichter and G. A. Johnson and S. R. Braddock and J. D.
Tobias},
Title = {Perioperative care of the child with the Johanson-Blizzard
syndrome},
Journal = {Paediatric Anaesthesia},
Volume = {13},
Number = {1},
Pages = {72 -- 75},
Year = {2003},
Month = {January},
Key = {Fichter03}
}
@article{fds174068,
Author = {CR Fichter and GA Johnson, SR Braddock and JD Tobias},
Title = {Perioperative care of the child with the Johanson-Blizzard
syndrome.},
Journal = {Paediatric anaesthesia},
Volume = {13},
Number = {1},
Pages = {72-5},
Year = {2003},
Month = {January},
ISSN = {1155-5645},
Keywords = {Abnormalities, Multiple • Anesthesia, Inhalation*
• Anesthetics, Inhalation • Child, Preschool
• Dwarfism • Female • Genes, Recessive •
Humans • Methyl Ethers • Nose • Syndrome
• abnormalities • complications •
surgery*},
Abstract = {The Johanson-Blizzard Syndrome (JBS) is an autosomal
recessive disorder with a characteristic phenotype,
including dwarfism, a beaked nose with aplastic alae nasi, a
high forehead, mid-line ectodermal scalp defects with sparse
hair and absent eyelashes/eyebrows, prominent scalp veins,
low set ears, a large anterior fontanelle, micrognathia,
thin lips, absent permanent dentition and microcephaly. In
addition to the characteristic facial features, associated
conditions include congenital heart disease,
exocrine/endocrine pancreatic dysfunction, hypothyroidism,
hypopituitarism, mental retardation, sensorineural hearing
loss and vesico-ureteral reflux. A case is presented and the
potential anaesthetic implications of this syndrome are
discussed.},
Language = {eng},
Key = {fds174068}
}
@article{fds174311,
Author = {W Veldkamp and HH Keasling and GA Johnson and WA Freyburger and RJ
Collins},
Title = {Pharmacologic studies with 3-(phenylpropoxy)guanidine
cyclohexanesulfamate.},
Journal = {Journal of pharmaceutical sciences},
Volume = {56},
Number = {7},
Pages = {829-33},
Year = {1967},
Month = {July},
ISSN = {0022-3549},
Keywords = {Animals • Appetite • Appetite Depressants •
Behavior, Animal • Blood Pressure • Body Weight
• Brain • Cats • Cyclohexanes •
Dextroamphetamine • Dogs • Drug Synergism •
Electric Stimulation • Electrophysiology •
Electroshock • Guanidines • Mice • Movement
• Norepinephrine • Reserpine • Serotonin
• Temperature • Tryptamines • drug effects
• drug effects* • metabolism • pharmacology
• pharmacology* • toxicity},
Language = {eng},
Key = {fds174311}
}
@article{fds204268,
Author = {M Lin and Y Qi and AF Chen and CT Badea and GA Johnson},
Title = {Phenylephrine-modulated cardiopulmonary blood flow measured
with use of X-ray digital subtraction angiography.},
Journal = {Journal of pharmacological and toxicological
methods},
Volume = {64},
Number = {2},
Pages = {180-6},
Year = {2011},
Month = {August},
ISSN = {1873-488X},
url = {http://dx.doi.org/10.1016/j.vascn.2011.08.001},
Abstract = {BACKGROUND: Cardiopulmonary blood flow is an important
indicator of organ function. Limitations in measuring blood
flow in live rodents suggest that rapid physiological
changes may be overlooked. For instance, relative
measurements limit imaging to whole organs or large sections
without adequately visualizing vasculature. Additionally,
current methods use small samples and invasive techniques
that often require killing animals, limiting sampling speed,
or both. A recently developed high spatial- and
temporal-resolution X-ray digital subtraction angiography
(DSA) system visualizes vasculature and measures blood flow
in rodents. This study was the first to use this system to
measure changes in cardiopulmonary blood flow in rats after
administering the vasoconstrictor phenylephrine. METHODS:
Cardiopulmonary blood flow and vascular anatomy were
assessed in 11 rats before, during, and after recovery from
phenylephrine. After acquiring DSA images at 12 time points,
a calibrated non-parametric deconvolution technique using
singular value decomposition (SVD) was applied to calculate
quantitative aortic blood flow in absolute metrics (mL/min).
Trans-pulmonary transit time was calculated as the time
interval between maximum signal enhancement in the pulmonary
trunk and aorta. Pulmonary blood volume was calculated based
on the central volume principle. Statistical analysis
compared differences in trans-pulmonary blood volume and
pressure, and aortic diameter using paired t-tests on
baseline, peak, and late-recovery time points. RESULTS:
Phenylephrine had dramatic qualitative and quantitative
effects on vascular anatomy and blood flow. Major vessels
distended significantly (aorta, ~1.2-times baseline) and
mean arterial blood pressure increased ~2 times. Pulmonary
blood volume, flow, pressure, and aortic diameter were not
significantly different between baseline and late recovery,
but differences were significant between baseline and peak,
as well as peak and recovery time points. CONCLUSIONS: The
DSA system with calibrated SVD technique acquired blood flow
measurements every 30s with a high level of regional
specificity, thus providing a new option for in vivo
functional assessment in small animals.},
Language = {eng},
Doi = {10.1016/j.vascn.2011.08.001},
Key = {fds204268}
}
@article{fds268809,
Author = {Lin, M and Qi, Y and Chen, AF and Badea, CT and Johnson,
GA},
Title = {Phenylephrine-modulated cardiopulmonary blood flow measured
with use of X-ray digital subtraction angiography.},
Journal = {J Pharmacol Toxicol Methods},
Volume = {64},
Number = {2},
Pages = {180-186},
Year = {2011},
url = {http://www.ncbi.nlm.nih.gov/pubmed/21846505},
Abstract = {INTRODUCTION: Cardiopulmonary blood flow is an important
indicator of organ function. Limitations in measuring blood
flow in live rodents suggest that rapid physiological
changes may be overlooked. For instance, relative
measurements limit imaging to whole organs or large sections
without adequately visualizing vasculature. Additionally,
current methods use small samples and invasive techniques
that often require killing animals, limiting sampling speed,
or both. A recently developed high spatial- and
temporal-resolution X-ray digital subtraction angiography
(DSA) system visualizes vasculature and measures blood flow
in rodents. This study was the first to use this system to
measure changes in cardiopulmonary blood flow in rats after
administering the vasoconstrictor phenylephrine. METHODS:
Cardiopulmonary blood flow and vascular anatomy were
assessed in 11 rats before, during, and after recovery from
phenylephrine. After acquiring DSA images at 12 time points,
a calibrated non-parametric deconvolution technique using
singular value decomposition (SVD) was applied to calculate
quantitative aortic blood flow in absolute metrics (mL/min).
Trans-pulmonary transit time was calculated as the time
interval between maximum signal enhancement in the pulmonary
trunk and aorta. Pulmonary blood volume was calculated based
on the central volume principle. Statistical analysis
compared differences in trans-pulmonary blood volume and
pressure, and aortic diameter using paired t-tests on
baseline, peak, and late-recovery time points. RESULTS:
Phenylephrine had dramatic qualitative and quantitative
effects on vascular anatomy and blood flow. Major vessels
distended significantly (aorta, ~1.2-times baseline) and
mean arterial blood pressure increased ~2 times. Pulmonary
blood volume, flow, pressure, and aortic diameter were not
significantly different between baseline and late recovery,
but differences were significant between baseline and peak,
as well as peak and recovery time points. DISCUSSION: The
DSA system with calibrated SVD technique acquired blood flow
measurements every 30s with a high level of regional
specificity, thus providing a new option for in vivo
functional assessment in small animals.},
Doi = {10.1016/j.vascn.2011.08.001},
Key = {fds268809}
}
@article{fds174245,
Author = {MM Joyce, JR Burghardt and RC Burghardt and RN Hooper and LA Jaeger and TE Spencer and FW Bazer and GA Johnson},
Title = {Pig conceptuses increase uterine interferon-regulatory
factor 1 (IRF1), but restrict expression to stroma through
estrogen-induced IRF2 in luminal epithelium.},
Journal = {Biology of reproduction},
Volume = {77},
Number = {2},
Pages = {292-302},
Year = {2007},
Month = {August},
ISSN = {0006-3363},
url = {http://dx.doi.org/10.1095/biolreprod.107.060939},
Keywords = {Animals • Endometrium • Epithelium •
Estradiol • Female • Fetus • Gene Expression
• In Situ Hybridization • Interferon Regulatory
Factor-1 • Interferon Regulatory Factor-2 •
Interferons • Pregnancy • RNA, Messenger •
Swine • Uterus • administration & dosage* •
analysis • chemistry • chemistry* • drug
effects • embryology* • genetics* •
pharmacology • physiology*},
Abstract = {Pig conceptuses secrete estrogen for pregnancy recognition,
and they secrete interferons (IFNs) gamma and delta during
the peri-implantation period. The uterine effects of pig
IFNs are not known, although ruminant conceptuses secrete
IFN tau for pregnancy recognition, and this increases the
expression of IFN-stimulated genes (ISGs) in the
endometrium. In sheep, the transcriptional repressor
interferon-regulatory factor 2 (IRF2) is expressed in the
endometrial luminal epithelium (LE) and appears to restrict
IFN tau induction of most ISGs, including IRF1, to the
stroma and glands. Interestingly, MX1, which is an ISG in
sheep, is also expressed in the endometrial stroma of
pregnant pigs. The objective of the present study was to
determine if estrogen and/or conceptus secretory proteins
(CSPs) that contain IFNs regulate IRF1 and IRF2 in pig
endometria. The endometrial levels of IRF1 and IRF2 were low
throughout the estrus cycle. After Day 12 of pregnancy, the
levels of the classical ISGs, which include IRF1, STAT2,
MIC, and B2M, increased in the overall endometrium, with
expression of IRF1 and STAT2 being specifically localized to
the stroma. IRF2 increased in the LE after Day 12. To
determine the effects of estrogen, pigs were treated with 17
beta-estradiol benzoate (E2). To determine the CSP effects,
pigs were treated with E2 and implanted with mini-osmotic
pumps that delivered control serum proteins (CX) to one
ligated uterine horn and CSP to the other horn. Estrogen
increased the level of IRF2 in the endometrial LE. The
administration of E2 and infusion of CSP increased the level
of IRF1 in the stroma. These results suggest that conceptus
estrogen induces IRF2 in the LE and limits the induction of
IRF1 by conceptus IFNs to the stroma. The cell-specific
expression of IRF1 and IRF2 in the pig endometrium
highlights the complex and overlapping events that are
associated with gene expression during the peri-implantation
period, when pregnancy recognition signaling and uterine
remodeling for implantation and placentation are necessary
for successful pregnancy.},
Language = {eng},
Doi = {10.1095/biolreprod.107.060939},
Key = {fds174245}
}
@article{fds174271,
Author = {MM Joyce and RC Burghardt and RD Geisert, JR Burghardt and RN Hooper and JW Ross, MD Ashworth and GA Johnson},
Title = {Pig conceptuses secrete estrogen and interferons to
differentially regulate uterine STAT1 in a temporal and cell
type-specific manner.},
Journal = {Endocrinology},
Volume = {148},
Number = {9},
Pages = {4420-31},
Year = {2007},
Month = {September},
ISSN = {0013-7227},
url = {http://dx.doi.org/10.1210/en.2007-0505},
Keywords = {Animals • Blastocyst • Embryo Implantation •
Embryonic Development • Estradiol • Female •
Gene Expression Regulation* • Pregnancy • STAT1
Transcription Factor • Swine • Uterus •
analogs & derivatives • cytology • cytology*
• drug effects • genetics* • pharmacology
• physiology • physiology*},
Abstract = {Conceptus trophectoderm and uterine luminal epithelial cells
interact via endocrine, paracrine, and autocrine modulators
to mediate pregnancy recognition and implantation. Pig
conceptuses not only release estrogens for pregnancy
recognition but also secrete interferons during
implantation. Because interferon-stimulated genes are
increased by interferons secreted for pregnancy recognition
in ruminants, we asked whether the interferon-stimulated
gene, STAT1, is up-regulated in pig endometrium by conceptus
estrogens and/or interferons. STAT1 expression in response
to day of pregnancy, estrogen injection, and intrauterine
infusion of conceptus secretory proteins in pigs indicated
1) estrogen increases STAT1 in luminal epithelial cells, 2)
conceptus secretory proteins that contain interferons
increase STAT1 in stroma, 3) STAT1 increases in close
proximity to the conceptus, and 4) early estrogen results in
conceptus death and no STAT1 in stroma. The interactions of
estrogen and interferons to regulate cell-type-specific
expression of STAT1 highlight the complex interplay between
endometrium and conceptus for pregnancy recognition and
implantation.},
Language = {eng},
Doi = {10.1210/en.2007-0505},
Key = {fds174271}
}
@booklet{Buhl89,
Author = {A. E. Buhl and T. T. Kawabe and D. J. Waldon and K. A.
Knight and G. A. Johnson and C. J. Walker and A. R.
Diani},
Title = {Pigmentation affects the distribution of mnx in
hair-follicles but does not influence its
activity},
Journal = {Clinical Research},
Volume = {37},
Number = {2},
Pages = {A745 -- A745},
Year = {1989},
Month = {April},
Key = {Buhl89}
}
@booklet{Buhl89a,
Author = {A. E. Buhl and T. T. Kawabe and D. J. Waldon and K. A.
Knight and G. A. Johnson and C. J. Walker and A. R.
Diani},
Title = {Pigmentation affects the distribution of mnx in
hair-follicles but does not influence its
activity},
Journal = {Journal Of Investigative Dermatology},
Volume = {92},
Number = {3},
Pages = {409 -- 409},
Year = {1989},
Month = {March},
Key = {Buhl89a}
}
@article{fds319488,
Author = {Macfall, JS and Johnson, GA},
Title = {Plants, seeds, roots, and soils as applications of magnetic
resonance microscopy},
Journal = {eMagRes},
Volume = {1},
Number = {1},
Pages = {147-154},
Publisher = {JOHN WILEY & SONS LTD},
Year = {2012},
Month = {January},
url = {http://dx.doi.org/10.1002/9780470034590.emrstm0396},
Abstract = {High-resolution magnetic resonance microscopy is discussed
as a useful tool in the study of plant structure and
physiology. In contrast to animals, plants have a relatively
homogenous distribution of water throughout the tissues.
High quality images highlighting both structural and
physiological differences can be acquired of plant
specimens, however, based on traditional MR parameters of
T1, T2 and susceptibility effects from air filled spaces.
Typical in plane resolutions of 5-40μm3 can be acquired,
showing significant contrasts between tissues. Additionally,
MR microscopy offers the ability to visualize and study
plant roots in situ, without removal from the soil
substrate. Applications of MR microscopy to plants includes
studies of developmental anatomy, physiological changes with
maturation, environmental physiology, pathologies, and the
study of intact roots in soil.},
Doi = {10.1002/9780470034590.emrstm0396},
Key = {fds319488}
}
@booklet{Robinson81,
Author = {D. S. Robinson and G. A. Johnson and J. Corcella and A. Nies and D. Howard and T. B. Cooper},
Title = {Plasma 3,4-dihydroxyphenylglycol, catecholamines, and
anti-depressant drug levels during treatment of
depression},
Journal = {Clinical Pharmacology \& Therapeutics},
Volume = {29},
Number = {2},
Pages = {277 -- 277},
Year = {1981},
Key = {Robinson81}
}
@article{fds174189,
Author = {DS Robinson and GA Johnson and A Nies and J Corcella and TB Cooper and D
Albright, D Howard},
Title = {Plasma levels of catecholamines and dihydroxyphenylglycol
during antidepressant drug treatment.},
Journal = {Journal of clinical psychopharmacology},
Volume = {3},
Number = {5},
Pages = {282-7},
Year = {1983},
Month = {October},
ISSN = {0271-0749},
Keywords = {Adult • Aged • Amitriptyline • Antidepressive
Agents • Blood Platelets • Catecholamines •
Clinical Trials as Topic • Depressive Disorder •
Double-Blind Method • Female • Glycols •
Humans • Male • Methoxyhydroxyphenylglycol •
Middle Aged • Monoamine Oxidase • Phenelzine
• Psychiatric Status Rating Scales • Time Factors
• analogs & derivatives • blood • blood*
• drug therapy • enzymology • therapeutic use
• therapeutic use*},
Abstract = {Plasma norepinephrine, epinephrine, and 3,4-dihydroxyphenylglycol
levels were measured in depressed outpatients treated in a
double-blind controlled clinical trial with 150 mg/day of
amitriptyline or 60 mg/day of phenelzine for 6 weeks. Both
antidepressant drug treatments were associated with a
significant decline in plasma dihydroxyphenylglycol
concentrations, which was more pronounced with phenelzine.
Plasma norepinephrine levels also declined during phenelzine
but not amitriptyline treatment, and the posttreatment
values correlated with clinical improvement with the
monoamine oxidase inhibiting drug. Reductions in
norepinephrine and dihydroxyphenylglycol correlated highly
with the degree of platelet monoamine oxidase inhibition.
Mechanisms of these antidepressant drug effects on amine
metabolism and their implications are discussed.},
Language = {eng},
Key = {fds174189}
}
@booklet{Johnson87b,
Author = {G. A. Johnson and V. J. Kapoor and P. G.
Young},
Title = {Plasma-deposited germanium nitride on indium-phosphide},
Journal = {Journal Of The Electrochemical Society},
Volume = {134},
Number = {8B},
Pages = {C430 -- C430},
Year = {1987},
Month = {August},
Key = {Johnson87b}
}
@booklet{Robinson83,
Author = {D. S. Robinson and G. A. Johnson and A. Nies and J. Corcella and T. B. Cooper and D. Albright and D. Howard},
Title = {Plasma-levels of catecholamines and dihydroxyphenylglycol
during anti-depressant drug-treatment},
Journal = {Journal Of Clinical Psychopharmacology},
Volume = {3},
Number = {5},
Pages = {282 -- 287},
Year = {1983},
Key = {Robinson83}
}
@article{fds174278,
Author = {GA Johnson},
Title = {Political Developments in Prehistory.},
Journal = {Science (New York, N.Y.)},
Volume = {216},
Number = {4548},
Pages = {867-869},
Year = {1982},
Month = {May},
ISSN = {1095-9203},
url = {http://dx.doi.org/10.1126/science.216.4548.867},
Language = {ENG},
Doi = {10.1126/science.216.4548.867},
Key = {fds174278}
}
@article{fds174085,
Author = {G Wu and FW Bazer and J Hu and GA Johnson and TE Spencer},
Title = {Polyamine synthesis from proline in the developing porcine
placenta.},
Journal = {Biology of reproduction},
Volume = {72},
Number = {4},
Pages = {842-50},
Year = {2005},
Month = {April},
ISSN = {0006-3363},
url = {http://dx.doi.org/10.1095/biolreprod.104.036293},
Keywords = {1-Pyrroline-5-Carboxylate Dehydrogenase • Amniotic
Fluid • Animals • Arginase • Carbon
Radioisotopes • Female • Fetal Development •
Male • Methionine • Ornithine •
Oxidoreductases Acting on CH-NH Group Donors • Placenta
• Polyamines • Pregnancy • Proline •
Putrescine • S-Adenosylmethionine • Spermidine
• Spermine • Sus scrofa • diagnostic use
• growth & development* • metabolism •
metabolism* • physiology*},
Abstract = {Polyamines (putrescine, spermidine, and spermine) are
essential for placental growth and angiogenesis. However,
little is known about polyamine synthesis in the porcine
placenta during conceptus development. The present study was
conducted to test the hypothesis that arginine and proline
are the major sources of ornithine for placental polyamine
production in pigs. Placentae, amniotic fluid, and allantoic
fluid were obtained from gilts on Days 20, 30, 35, 40, 45,
50, 60, 90, and 110 of the 114-day gestation (n = 6 per
day). Placentae as well as amniotic and allantoic fluids
were analyzed for arginase, proline oxidase, ornithine
aminotransferase (OAT), ornithine decarboxylase (ODC),
proline transport, concentrations of amino acids and
polyamines, and polyamine synthesis using established
radiochemical and chromatographic methods. Neither arginase
activity nor conversion of arginine into polyamines was
detected in the porcine placenta. In contrast, both proline
and ornithine were converted into putrescine, spermidine,
and spermine in placental tissue throughout pregnancy. The
activities of proline oxidase, OAT, and ODC as well as
proline transport, polyamine synthesis from proline, and
polyamine concentrations increased markedly between Days 20
and 40 of gestation, declined between Days 40 and 90 of
gestation, and remained at the reduced level through Day 110
of gestation. Proline oxidase and OAT, but not arginase,
were present in allantoic and amniotic fluids for the
production of ornithine (the immediate substrate for
polyamine synthesis). The activities of these two enzymes as
well as the concentrations of ornithine and total polyamines
in fetal fluids were highest at Day 40 but lowest at Days
20, 90, and 110 of gestation. These results indicate that
proline is the major amino acid for polyamine synthesis in
the porcine placenta and that the activity of this synthetic
pathway is maximal during early pregnancy, when placental
growth is most rapid. Our novel findings provide a new base
of information for future studies to define the role of
proline in fetoplacental growth and development.},
Language = {eng},
Doi = {10.1095/biolreprod.104.036293},
Key = {fds174085}
}
@booklet{Wu04,
Author = {G. Y. Wu and T. E. Spencer and G. A. Johnson and F. W.
Bazer},
Title = {Polyamine synthesis from proline in the developing porcine
placenta.},
Journal = {Biology Of Reproduction},
Pages = {158 -- 158},
Year = {2004},
Key = {Wu04}
}
@article{fds268819,
Author = {Veraart, J and Leergaard, TB and Antonsen, BT and Van Hecke and W and Blockx, I and Jeurissen, B and Jiang, Y and Van der Linden and A and Johnson, GA and Verhoye, M and Sijbers, J},
Title = {Population-averaged diffusion tensor imaging atlas of the
Sprague Dawley rat brain.},
Journal = {Neuroimage},
Volume = {58},
Number = {4},
Pages = {975-983},
Year = {2011},
Month = {October},
ISSN = {1095-9572},
url = {http://www.ncbi.nlm.nih.gov/pubmed/21749925},
Keywords = {Algorithms • Animals • Atlases as Topic* •
Brain • Brain Mapping • Diffusion Tensor Imaging
• Magnetic Resonance Imaging • Male •
Nonlinear Dynamics • Rats • Rats, Sprague-Dawley
• anatomy & histology* • methods*},
Abstract = {Rats are widely used in experimental neurobiological
research, and rat brain atlases are important resources for
identifying brain regions in the context of experimental
microsurgery, tissue sampling, and neuroimaging, as well as
comparison of findings across experiments. Currently, most
available rat brain atlases are constructed from
histological material derived from single specimens, and
provide two-dimensional or three-dimensional (3D) outlines
of diverse brain regions and fiber tracts. Important
limitations of such atlases are that they represent
individual specimens, and that finer details of tissue
architecture are lacking. Access to more detailed 3D brain
atlases representative of a population of animals is needed.
Diffusion tensor imaging (DTI) is a unique neuroimaging
modality that provides sensitive information about
orientation structure in tissues, and is widely applied in
basic and clinical neuroscience investigations. To
facilitate analysis and assignment of location in rat brain
neuroimaging investigations, we have developed a
population-averaged three-dimensional DTI atlas of the
normal adult Sprague Dawley rat brain. The atlas is
constructed from high resolution ex vivo DTI images, which
were nonlinearly warped into a population-averaged in vivo
brain template. The atlas currently comprises a selection of
manually delineated brain regions, the caudate-putamen
complex, globus pallidus, entopeduncular nucleus, substantia
nigra, external capsule, corpus callosum, internal capsule,
cerebral peduncle, fimbria of the hippocampus, fornix,
anterior commisure, optic tract, and stria terminalis. The
atlas is freely distributed and potentially useful for
several purposes, including automated and manual delineation
of rat brain structural and functional imaging
data.},
Language = {eng},
Doi = {10.1016/j.neuroimage.2011.06.063},
Key = {fds268819}
}
@article{fds341988,
Author = {Calabrese, E and Adil, SM and Cofer, G and Perone, CS and Cohen-Adad, J and Lad, SP and Johnson, GA},
Title = {Postmortem diffusion MRI of the entire human spinal cord at
microscopic resolution.},
Journal = {Neuroimage Clin},
Volume = {18},
Pages = {963-971},
Year = {2018},
url = {http://dx.doi.org/10.1016/j.nicl.2018.03.029},
Abstract = {The human spinal cord is a central nervous system structure
that plays an important role in normal motor and sensory
function, and can be affected by many debilitating
neurologic diseases. Due to its clinical importance, the
spinal cord is frequently the subject of imaging research.
Common methods for visualizing spinal cord anatomy and
pathology include histology and magnetic resonance imaging
(MRI), both of which have unique benefits and drawbacks.
Postmortem microscopic resolution MRI of fixed specimens,
sometimes referred to as magnetic resonance microscopy
(MRM), combines many of the benefits inherent to both
techniques. However, the elongated shape of the human spinal
cord, along with hardware and scan time limitations, have
restricted previous microscopic resolution MRI studies (both
in vivo and ex vivo) to small sections of the cord. Here we
present the first MRM dataset of the entire postmortem human
spinal cord. These data include 50 μm isotropic
resolution anatomic image data and 100 μm isotropic
resolution diffusion data, made possible by a 280 h long
multi-segment acquisition and automated image segment
composition. We demonstrate the use of these data for spinal
cord lesion detection, automated volumetric gray matter
segmentation, and quantitative spinal cord morphometry
including estimates of cross sectional dimensions and gray
matter fraction throughout the length of the
cord.},
Doi = {10.1016/j.nicl.2018.03.029},
Key = {fds341988}
}
@article{fds292755,
Author = {Calabrese, E and Hickey, P and Hulette, C and Zhang, J and Parente, B and Lad, SP and Johnson, GA},
Title = {Postmortem Diffusion MRI of the Human Brainstem and Thalamus
for Deep Brain Stimulator Electrode Localization},
Journal = {MOVEMENT DISORDERS},
Volume = {30},
Number = {10},
Pages = {E6-E6},
Publisher = {WILEY-BLACKWELL},
Year = {2015},
Month = {September},
ISSN = {0885-3185},
url = {http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000360759500015&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=47d3190e77e5a3a53558812f597b0b92},
Key = {fds292755}
}
@article{fds268696,
Author = {Calabrese, E and Hickey, P and Hulette, C and Zhang, J and Parente, B and Lad, SP and Johnson, GA},
Title = {Postmortem diffusion MRI of the human brainstem and thalamus
for deep brain stimulator electrode localization.},
Journal = {Hum Brain Mapp},
Volume = {36},
Number = {8},
Pages = {3167-3178},
Year = {2015},
Month = {August},
ISSN = {1065-9471},
url = {http://dx.doi.org/10.1002/hbm.22836},
Abstract = {Deep brain stimulation (DBS) is an established surgical
therapy for medically refractory tremor disorders including
essential tremor (ET) and is currently under investigation
for use in a variety of other neurologic and psychiatric
disorders. There is growing evidence that the anti-tremor
effects of DBS for ET are directly related to modulation of
the dentatorubrothalamic tract (DRT), a white matter pathway
that connects the cerebellum, red nucleus, and ventral
intermediate nucleus of the thalamus. Emerging white matter
targets for DBS, like the DRT, will require improved
three-dimensional (3D) reference maps of deep brain anatomy
and structural connectivity for accurate electrode
targeting. High-resolution diffusion MRI of postmortem brain
specimens can provide detailed volumetric images of
important deep brain nuclei and 3D reconstructions of white
matter pathways with probabilistic tractography techniques.
We present a high spatial and angular resolution diffusion
MRI template of the postmortem human brainstem and thalamus
with 3D reconstructions of the nuclei and white matter
tracts involved in ET circuitry. We demonstrate registration
of these data to in vivo, clinical images from patients
receiving DBS therapy, and correlate electrode proximity to
tractography of the DRT with improvement of ET
symptoms.},
Doi = {10.1002/hbm.22836},
Key = {fds268696}
}
@booklet{Gray03,
Author = {C. A. Gray and M. D. Stewart and G. A. Johnson and T. E.
Spencer},
Title = {Postpartum uterine involution in sheep: histoarchitecture
and changes in endometrial gene expression},
Journal = {Reproduction},
Volume = {125},
Number = {2},
Pages = {185 -- 198},
Year = {2003},
Month = {February},
Key = {Gray03}
}
@article{fds174285,
Author = {CA Gray, MD Stewart and GA Johnson and TE Spencer},
Title = {Postpartum uterine involution in sheep: histoarchitecture
and changes in endometrial gene expression.},
Journal = {Reproduction (Cambridge, England)},
Volume = {125},
Number = {2},
Pages = {185-98},
Year = {2003},
Month = {February},
ISSN = {1470-1626},
Keywords = {Animals • Apoptosis • B-Lymphocytes •
Epithelium • Estradiol • Estrogen Receptor alpha
• Female • Glycoproteins • Hysterectomy
• Immunohistochemistry • In Situ Hybridization
• Organ Size • Ovariectomy • Placenta •
Postpartum Period • Pregnancy • Progesterone
• RNA, Messenger • Receptors, Estrogen •
Receptors, Oxytocin • Receptors, Progesterone •
Receptors, Prolactin • Serpins* • Sheep •
T-Lymphocytes • Uterus • analysis • anatomy &
histology • anatomy & histology* • blood •
cytology • genetics • immunology • metabolism
• physiology*},
Abstract = {After parturition, the uterus undergoes marked remodelling
during involution; however, little is known of the hormonal,
cellular and molecular mechanisms that regulate this
process. The working hypothesis used in this study is that
return of the ovine uterus to a non-pregnant state involves
termination of a hormonal servomechanism that regulates
endometrial gland morphogenesis and function during
pregnancy. Suffolk ewes were ovariohysterectomized on
postpartum days 1, 7, 14 or 28. Serum concentrations of
oestradiol were high at parturition, declined to postpartum
day 4, peaked on postpartum day 6, and then declined and
remained low thereafter. Progesterone was undetectable in
plasma from ewes post partum. Uterine wet mass and horn
length decreased after postpartum day 1, but ovarian mass
did not change. Residual placental cotyledons were present
in the maternal caruncles on postpartum days 1 and 7 and
were extruded by postpartum day 14 as plaques that were
resorbed by postpartum day 28. The width of the total
endometrium, stratum compactum, stratum spongiosum and
myometrium, as well as endometrial gland density, decreased
after parturition. Most apoptotic cells in the involuting
uterus were large, vacuolated and located between the
endometrial glandular epithelial cells on postpartum days 1
and 7. Immunofluorescence analyses identified both T and B
cells within the glandular epithelium on postpartum day 1.
Cell proliferation was detected in the luminal epithelium
and glandular epithelium on postpartum days 1 and 7. On
postpartum day 1, expression of oestrogen receptor alpha
(ERalpha) was not detected in luminal epithelium and was low
in glandular epithelium, but ERalpha was present in
epithelia thereafter. Progesterone receptor (PR) protein was
not detected in endometrial epithelia on postpartum day 1,
but was detected in the glandular epithelium thereafter.
Between postpartum days 1 and 7, ERalpha and PR protein
increased substantially in the endometrial glandular
epithelium. On postpartum days 1-28, abundant expression of
oxytocin receptor mRNA was detected in endometrial luminal
epithelium and superficial to the middle glandular
epithelium. Prolactin receptor (PRLR) mRNA was detected in
glandular epithelium on all postpartum days, whereas mRNA
for uterine milk protein (UTMP), an index of secretory
capacity of glandular epithelium, was present only on
postpartum day 1. Collectively, these results indicate that
uterine involution in ewes involves remodelling of both
caruncular and intercaruncular areas of the uterine wall and
termination of differentiated uterine gland functions
characteristic of pregnancy.},
Language = {eng},
Key = {fds174285}
}
@booklet{Buhl92a,
Author = {A. E. Buhl and D. J. Waldon and S. J. Conrad and M. J.
Mulholland and K. L. Shull and M. F. Kubicek and G. A.
Johnson and M. N. Brunden and K. J. Stefanski and R. G.
Stehle and R. C. Gadwood and B. V. Kamdar and L. M. Thomasco and H. J. Schostarez and T.},
Title = {Potassium channel conductance - a mechanism affecting
hair-growth both invitro and invivo},
Journal = {Journal Of Investigative Dermatology},
Volume = {98},
Number = {3},
Pages = {315 -- 319},
Year = {1992},
Month = {March},
Key = {Buhl92a}
}
@article{fds174224,
Author = {AE Buhl and DJ Waldon and SJ Conrad and MJ Mulholland and KL Shull and MF
Kubicek, GA Johnson and MN Brunden and KJ Stefanski and RG
Stehle},
Title = {Potassium channel conductance: a mechanism affecting hair
growth both in vitro and in vivo.},
Journal = {The Journal of investigative dermatology},
Volume = {98},
Number = {3},
Pages = {315-9},
Year = {1992},
Month = {March},
ISSN = {0022-202X},
Keywords = {Animals • Benzopyrans • Cells, Cultured •
Cromakalim • Hair • Mice • Mice, Inbred C3H
• Mice, Inbred C57BL • Minoxidil • Potassium
Channels • Pyrroles • growth & development* •
pharmacology • physiology*},
Abstract = {The opening of intracellular potassium channels has been
suggested as a mechanism regulating hair growth. Enhancing
the flux of potassium ions is a mechanism shared by several
structurally diverse antihypertensive agents including
minoxidil sulfate (the active metabolite of minoxidil),
pinacidil, P-1075 (a potent pinacidil analog), RP-49,356,
diazoxide, cromakalim, and nicorandil. Of these drugs,
minoxidil, pinacidil, and diazoxide have been reported to
elicit hypertrichosis in humans. This potassium channel
hypothesis was examined by testing these drugs for effects
on hair growth both in vitro and in vivo. For the in vitro
studies, mouse vibrissae follicles were cultured for 3 d
with drug and the effects on hair growth were measured by
metabolic labeling. All drugs, except diazoxide, enhanced
cysteine incorporation into the hair shafts of the cultured
vibrissae. Diazoxide was poorly soluble and thus was tested
only at low doses. Minoxidil, P-1075, cromakalim, and
RP-49,356 were also evaluated in vivo by measuring hair
growth effects in balding stumptail macaque monkeys. The
drugs were administered topically to defined sites on
balding scalps once per day for 4-5 months and the amount of
hair grown was determined by monthly measurements of shaved
hair weight. Three of the drugs produced significant
increases in hair weight whereas, the RP-49,356 had no
effect. These studies provide correlative evidence that the
opening of potassium channels is an important regulatory
mechanism for hair growth. This provides the impetus for
further studies on this potentially important mechanism
affecting hair biology.},
Language = {eng},
Key = {fds174224}
}
@booklet{Johnson82,
Author = {Johnson, GA},
Title = {Practical approach to quality assurance in computed
tomography},
Journal = {Proceedings of SPIE - The International Society for Optical
Engineering},
Volume = {347},
Pages = {148-154},
Year = {1982},
Month = {December},
url = {http://dx.doi.org/10.1117/12.933821},
Abstract = {The goal of tissue characterization via quantitative
computed tomography can only be accomplished with careful
control of certain parameters. A quality assurance program
is described which includes daily measurement of certain
essential elements e.g. Hounsfield conversion, linearity,
noise and resolution. Secondary measurements of collimation,
bed movement, dose, and sensitivity profiles are performed
on a monthly basis. Daily measurements are accomplished in
less than 15 minutes while monthly checks take less than 1.5
hr thus minimizing conflict with a busy clinical routine.
Examples of rapid recording methods and measurement
techniques are included. © 1982 SPIE.},
Doi = {10.1117/12.933821},
Key = {Johnson82}
}
@booklet{Johnson80b,
Author = {JOHNSON, GA and HOLT, DC and EFFMANN, EL},
Title = {PRACTICAL GUIDELINES FOR SPECIMEN RADIOGRAPHY},
Journal = {INVESTIGATIVE RADIOLOGY},
Volume = {15},
Number = {5},
Pages = {377-377},
Year = {1980},
ISSN = {0020-9996},
url = {http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:A1980KK17000009&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=47d3190e77e5a3a53558812f597b0b92},
Key = {Johnson80b}
}
@booklet{Macfall91a,
Author = {MacFall, JR and Maki, JH and Johnson, GA and Hedlund, LW and Cofer,
GP},
Title = {Pre- and postmortem diffusion coefficients in rat neural and
muscle tissues.},
Journal = {Magn Reson Med},
Volume = {20},
Number = {1},
Pages = {89-99},
Year = {1991},
Month = {July},
ISSN = {0740-3194},
url = {http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:A1991FU08200009&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=47d3190e77e5a3a53558812f597b0b92},
Abstract = {Pulsed gradient diffusion-weighted spin-echo images (7 to 11
gradient strengths) were obtained in a coronal slice through
the midbrain for five normal adult white rats before and
after sacrifice in a 2-T CSI system with air temperature
control. The pulse sequence was cardiac gated and
respiratory synchronized in order to minimize motion
artifacts (Tr greater than 2 s. Te = 30 ms). Diffusion
coefficients reflecting several tissue compartments (D*) in
brain and muscle were calculated and referenced to
simultaneously imaged tubes of water. In the living animals,
brain cortical matter had a value of D* = (0.82 +/- 0.02) x
10(-3) mm2/s. deeper brain regions had a value of D* = (0.73
+/- 0.02) x 10(-3) mm2/s, and the muscle had a value of D* =
(1.4 +/- 0.1) x 10(-3) mm2/s. Postmortem the values in brain
dropped by approximately 30%, while remaining constant in
muscle. Signal intensity in the spin-echo images for muscle
tissue rose by 50% over a 1- to 2-h interval after sacrifice
while that of brain tissue remained relatively
stable.},
Doi = {10.1002/mrm.1910200110},
Key = {Macfall91a}
}
@booklet{Winter85,
Author = {WINTER, TC and JOHNSON, GA and MACFALL, JR},
Title = {PRECISION AND ACCURACY IN THE MEASUREMENT OF T1 ON AN
IMAGING-SYSTEM},
Journal = {INVESTIGATIVE RADIOLOGY},
Volume = {20},
Number = {6},
Pages = {S14-S14},
Publisher = {LIPPINCOTT-RAVEN PUBL},
Year = {1985},
Month = {January},
ISSN = {0020-9996},
url = {http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:A1985ARG0500074&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=47d3190e77e5a3a53558812f597b0b92},
Key = {Winter85}
}
@booklet{Micheli02,
Author = {F. Micheli and C. H. Peterson and L. S. Mullineaux and C. R.
Fisher and S. W. Mills and G. Sancho and G. A. Johnson and H. S. Lenihan},
Title = {Predation structures communities at deep-sea hydrothermal
vents},
Journal = {Ecological Monographs},
Volume = {72},
Number = {3},
Pages = {365 -- 382},
Year = {2002},
Month = {August},
Key = {Micheli02}
}
@article{fds359546,
Author = {Lu, H and Pollack, E and Young, R and Babb, JS and Johnson, G and Zagzag,
D and Carson, R and Jensen, JH and Helpern, JA and Law,
M},
Title = {Predicting grade of cerebral glioma using vascular-space
occupancy MR imaging.},
Journal = {AJNR Am J Neuroradiol},
Volume = {29},
Number = {2},
Pages = {373-378},
Year = {2008},
Month = {February},
url = {http://dx.doi.org/10.3174/ajnr.A0794},
Abstract = {BACKGROUND AND PURPOSE: MR imaging can measure tissue
perfusion and the integrity of the blood-brain barrier. We
hypothesize that a combined measure of cerebral blood volume
and vascular permeability using vascular-space occupancy
(VASO) MR imaging, a recently developed imaging technique,
is of diagnostic value for predicting tumor grade. MATERIALS
AND METHODS: Thirty-nine patients (9 World Health
Organization [WHO] grade II, 20 grade III, and 10 grade IV
as determined by histopathologic assessment) were examined
using VASO MR imaging, and regions-of-interest analysis was
performed in tumoral regions, as well as in regions
contralateral to the tumor. A Mann-Whitney test was
conducted on the resulting VASO indices for a pairwise
comparison across tumor grades. Nominal logistic regression
was used to evaluate the use of VASO parameters for
predicting group membership (by the percentage of correct
classifications). RESULTS: The ratio between tumor side and
contralateral side, VASO(Ratio), showed significant
differences in all 3 of the pairwise comparisons (P < .01).
VASO values in the tumoral regions, VASO(Tumor), showed
significant difference between grade II and III and between
II and IV but not between III and IV. Both VASO(Tumor) and
VASO(Ratio) were found to be significant predictors of tumor
grade, giving diagnostic accuracies of 66.7% and 71.8%,
respectively. When testing to discriminate grade II tumors
from higher grade tumors, the areas under the receiver
operating characteristic curve were found to be 0.974 and
0.985 for VASO(Tumor) and VASO(Ratio), respectively.
CONCLUSION: VASO MR imaging can be used for noninvasive
tumor grade prediction based on cerebral blood volume and
vascular permeability. VASO is more effective in separating
WHO grade II from higher grades than in separating grade III
from grade IV.},
Doi = {10.3174/ajnr.A0794},
Key = {fds359546}
}
@booklet{Choi03,
Author = {Y. Choi and G. A. Johnson and T. E. Spencer and F. W.
Bazer},
Title = {Pregnancy and interferon tau regulate major
histocompatibility complex class I and beta(2)-microglobulin
expression in the ovine uterus},
Journal = {Biology Of Reproduction},
Volume = {68},
Number = {5},
Pages = {1703 -- 1710},
Year = {2003},
Month = {May},
Key = {Choi03}
}
@article{fds174102,
Author = {Y Choi and GA Johnson and TE Spencer and FW Bazer},
Title = {Pregnancy and interferon tau regulate major
histocompatibility complex class I and beta2-microglobulin
expression in the ovine uterus.},
Journal = {Biology of reproduction},
Volume = {68},
Number = {5},
Pages = {1703-10},
Year = {2003},
Month = {May},
ISSN = {0006-3363},
url = {http://dx.doi.org/10.1095/biolreprod.102.012708},
Keywords = {Animals • Endometrium • Estrous Cycle •
Female • Fluorescent Antibody Technique • Gene
Expression Regulation, Developmental • Genes, MHC Class
I • In Situ Hybridization • Interferon Type I
• Pregnancy • Pregnancy Proteins • Pregnancy,
Animal • RNA, Messenger • Sheep • Uterus
• beta 2-Microglobulin • biosynthesis •
biosynthesis* • genetics • genetics* •
metabolism • metabolism* • physiology •
physiology*},
Abstract = {Major histocompatibility complex (MHC) class I molecules,
consisting of an alpha chain and beta2-microglobulin
(beta2MG), play an important role in immune rejection
responses by discriminating self and nonself and are
increased by type I interferons during antiviral responses.
Interferon tau (IFNtau), the pregnancy-recognition signal in
ruminants, is a type I interferon produced by the ovine
conceptus between Days 11 and 21 of gestation. In study 1,
expression of MHC class I alpha chain and beta2MG mRNA and
protein was detected primarily in endometrial luminal
epithelium (LE) and glandular epithelium (GE) on Days 10 and
12 of the estrous cycle and pregnancy. On Days 14-20 of
pregnancy, MHC class I and beta2MG expression increased only
in endometrial stroma and GE and, concurrently, was absent
in LE and superficial ductal GE (sGE). Although neither MHC
class I nor beta2MG proteins were detected in Day 20
trophectoderm, beta2MG mRNA was detected in conceptus
trophectoderm. In study 2, cyclic ewes were ovariectomized
on Day 5, treated daily with progesterone to Day 16,
received intrauterine infusions between Days 11 and 16 of
either control serum proteins or recombinant ovine IFNtau,
and were hysterectomized on Day 17. The IFNtau increased MHC
class I and beta2MG expression only in endometrial stroma
and GE. During pregnancy, MHC class I and beta2MG gene
expression is inhibited in endometrial LE and sGE but,
paradoxically, is stimulated by IFNtau in the stroma and GE.
The silencing of MHC class I alpha chain and beta2MG genes
in the endometrial LE and sGE during pregnancy recognition
and establishment may be a critical mechanism preventing
immune rejection of the conceptus allograft.},
Language = {eng},
Doi = {10.1095/biolreprod.102.012708},
Key = {fds174102}
}
@booklet{Johnson98c,
Author = {G. A. Johnson and K. J. Austin and E. A. van Kirk and T. R.
Hansen},
Title = {Pregnancy and interferon-tau induce conjugation of bovine
ubiquitin cross-reactive protein to cytosolic uterine
proteins},
Journal = {Biology Of Reproduction},
Volume = {58},
Number = {4},
Pages = {898 -- 904},
Year = {1998},
Month = {April},
Key = {Johnson98c}
}
@article{fds174288,
Author = {GA Johnson and KJ Austin and EA Van Kirk and TR Hansen},
Title = {Pregnancy and interferon-tau induce conjugation of bovine
ubiquitin cross-reactive protein to cytosolic uterine
proteins.},
Journal = {Biology of reproduction},
Volume = {58},
Number = {4},
Pages = {898-904},
Year = {1998},
Month = {April},
ISSN = {0006-3363},
Keywords = {Amino Acid Sequence • Animals • Blotting, Western
• Carrier Proteins • Cattle • Cytosol •
Densitometry • Female • Interferon Type I •
Molecular Sequence Data • Pregnancy • Pregnancy
Proteins • Pregnancy, Animal • Proteins •
Recombinant Proteins • Ubiquitins • Uterus •
analogs & derivatives* • chemistry • chemistry*
• metabolism • metabolism* • pharmacology
• pharmacology* • physiology* •
ultrastructure},
Abstract = {Conceptus-derived interferon-tau (IFN-tau) induces bovine
endometrial ubiquitin cross-reactive protein (UCRP) mRNA and
protein on Days 15-21 of pregnancy. Bovine UCRP retains the
Leu-Arg-Gly-Gly C-terminal sequence of ubiquitin that
ligates to and directs degradation of cytosolic proteins.
The objectives of the present experiments were to determine
whether UCRP became conjugated to endometrial cytosolic
proteins during early pregnancy and in response to
recombinant bovine (rbo) IFN-tau. Ubiquitin (8 kDa), UCRP
(17 kDa), and conjugates thereof (> or = 30 kDa) were
quantitated using Western blotting and densitometry.
Endometrial ubiquitin and its conjugates did not differ
between Day 18 pregnant and nonpregnant cows, or between
control and rboIFN-tau-treated (25 nM) explant cultures (Day
14; nonpregnant). Bovine UCRP was induced in endometrium
from pregnant as compared with nonpregnant cows. Conjugation
of endometrial proteins to UCRP was induced in pregnant as
compared to nonpregnant cows. Recombinant boIFN-tau induced
UCRP and its conjugates in cultured endometrial explants
from nonpregnant cows. It is concluded that UCRP, in
response to rboIFN-tau, becomes conjugated to endometrial
cytosolic proteins during early pregnancy. The regulation of
uterine proteins by UCRP may be integral to the maintenance
of early pregnancy in ruminants.},
Language = {eng},
Key = {fds174288}
}
@booklet{Johnson97,
Author = {G. A. Johnson and K. J. Austin and E. A. Vankirk and T. R.
Hansen},
Title = {Pregnancy and interferon-tau induce conjugation of bovine
ubiquitin cross-reactive protein to cytosolic uterine
proteins.},
Journal = {Biology Of Reproduction},
Volume = {56},
Pages = {160 -- 160},
Year = {1997},
Key = {Johnson97}
}
@article{fds174123,
Author = {TE Spencer and GA Johnson and FW Bazer and RC Burghardt and M
Palmarini},
Title = {Pregnancy recognition and conceptus implantation in domestic
ruminants: roles of progesterone, interferons and endogenous
retroviruses.},
Journal = {Reproduction, fertility, and development},
Volume = {19},
Number = {1},
Pages = {65-78},
Year = {2007},
ISSN = {1031-3613},
Keywords = {Animals • Cattle • Embryo Implantation* •
Endogenous Retroviruses • Endometrium • Female
• Gene Expression Regulation, Developmental •
Interferons • Pregnancy • Pregnancy, Animal •
Progesterone • Receptors, Progesterone • Ruminants
• Sheep • Uterus • embryology •
embryology* • metabolism • physiology •
physiology*},
Abstract = {The present review highlights new information on pregnancy
recognition and conceptus development and implantation in
sheep with respect to regulation by progesterone,
interferons and endogenous retroviruses. After formation of
the corpus luteum, progesterone acts on the endometrium and
stimulates blastocyst growth and elongation to a filamentous
conceptus (embryo/fetus and associated extra-embryonic
membranes). The envelope of endogenous retroviruses related
to Jaagsiekte sheep retroviruses appears to intrinsically
regulate mononuclear trophectoderm cell proliferation and
differentiation into trophoblast giant binucleate cells. The
mononuclear trophectoderm cells of elongating sheep
conceptuses secrete interferon-tau, which acts on the
endometrium to prevent development of the luteolytic
mechanism by inhibiting transcription of the gene for the
oestrogen receptor alpha in the luminal and superficial
ductal glandular epithelia. These actions prevent
oestrogen-induced transcription of the oxytocin receptor
gene and, therefore, oxytocin-induced luteolytic pulses of
prostaglandin F2alpha. Progesterone down regulation of its
receptors in luminal and glandular epithelia correlates
temporally with a reduction in anti-adhesive mucin land
induction of secreted galectin 15 (LGALSI5) and secreted
phosphoprotein 1, which are proposed to regulate
trophectoderm proliferation and adhesion. Interferon-c acts
on the endometrial lumenal epithelium to induce WNT7A and to
stimulate LGALS 15, cathepsin L and cystatin C, which are
candidate regulators of conceptus development and
implantation. The number of potential contributors to
maternal recognition and establishment of pregnancy
continues to grow and this highlights our limited
appreciation of the complexity of the key molecules and
signal transduction pathways that intersect during these key
developmental processes. The goal of improving reproductive
efficiency by preventing embryonic losses that occur during
the peri-implantation period of pregnancy in domestic
ruminants provides the challenge to increase our knowledge
of endometrial function and conceptus development.},
Language = {eng},
Key = {fds174123}
}
@booklet{Johnson99g,
Author = {G. A. Johnson and A. Calkins},
Title = {Prehospital triage and communication performance in small
mass casualty incidents: A gauge for disaster
preparedness},
Journal = {American Journal Of Emergency Medicine},
Volume = {17},
Number = {2},
Pages = {148 -- 150},
Year = {1999},
Month = {March},
Key = {Johnson99g}
}
@article{fds174119,
Author = {GA Johnson and A Calkins},
Title = {Prehospital triage and communication performance in small
mass casualty incidents: a gauge for disaster
preparedness.},
Journal = {The American journal of emergency medicine},
Volume = {17},
Number = {2},
Pages = {148-50},
Year = {1999},
Month = {March},
ISSN = {0735-6757},
Keywords = {Accidents, Traffic • Disaster Planning* •
Emergency Medical Services* • Humans • New York
• Outcome and Process Assessment (Health Care) •
Prospective Studies • Quality Control • Relief
Work* • Retrospective Studies • Triage* •
Wounds and Injuries • etiology •
therapy},
Abstract = {Because of their infrequency, disasters are difficult to
train for. Emergency prehospital personnel frequently
participate in small mass casualty incidents (MCIs) (3 to 50
victims). This study sought to examine prehospital
performance in small MCIs in areas that are frequently
mismanaged in disasters. Prospective data from the resource
physician and retrospective data from tape recorded
prehospital conversations were collected for a 9-month
period. Clinical patient data, patient demographics,
emergency medical services squad characteristics, and triage
information were recorded. Forty-five consecutive MCIs were
studied. Most of these were motor vehicle accidents.
Prehospital providers included paid providers, nonpaid
providers, and air and ground transport. The mean number of
victims first identified (4.6%) was greatly different than
the mean number of victims eventually transported from a
scene (7.1%). Most patients were treated at a level 1 trauma
center. Frequent errors included having multiple
communicators on site (38%), misidentifying the number of
victims (56%), and having unclear information for the
resource physician (43%). Only 38% of events had prehospital
triage information that was deemed appropriate in total.
These results show that scene and triage errors are frequent
in MCIs of small scale. This information can be used to
assay a system's readiness for disasters.},
Language = {eng},
Key = {fds174119}
}
@article{fds174176,
Author = {JW Ross, MD Ashworth and FJ White and GA Johnson and PJ Ayoubi and U
DeSilva, KM Whitworth and RS Prather and RD Geisert},
Title = {Premature estrogen exposure alters endometrial gene
expression to disrupt pregnancy in the pig.},
Journal = {Endocrinology},
Volume = {148},
Number = {10},
Pages = {4761-73},
Year = {2007},
Month = {October},
ISSN = {0013-7227},
url = {http://dx.doi.org/10.1210/en.2007-0599},
Keywords = {Abortion, Spontaneous • Aldehyde Reductase •
Animals • Antigens, CD24 • Drug Administration
Schedule • Embryo Loss • Endometrium •
Epithelium • Estradiol • Female • Gene
Expression • Gene Expression Profiling •
Gestational Age • Neurokinin B • Oligonucleotide
Array Sequence Analysis • Osteopontin • Pregnancy
• Pregnancy, Animal • RNA, Messenger • Swine
• Time Factors • Uterus • administration &
dosage • analogs & derivatives • analogs &
derivatives* • drug effects* • etiology* •
genetics • metabolism • metabolism* •
pharmacology},
Abstract = {Establishment and maintenance of pregnancy in the pig
involve intricate communication between the developing
conceptuses and maternal endometrium. Conceptus synthesis
and release of estrogen during trophoblastic elongation are
essential factors involved with establishing
conceptus-uterine communication. The present study
identified endometrial changes in gene expression associated
with implantation failure and complete pregnancy loss after
premature exposure of pregnant gilts to exogenous estrogen.
Gilts were treated with either 5 mg estradiol cypionate (EC)
or corn oil on d-9 and -10 gestation, which was associated
with complete conceptus degeneration by d-17 gestation.
Microarray analysis of gene expression revealed that a total
of eight, 32, and five genes were up-regulated in the EC
endometrium, whereas one, 39, and 16 genes were
down-regulated, on d 10, 13, and 15, respectively. Four
endometrial genes altered by EC, aldose reductase (AKR1B1),
secreted phosphoprotein 1 (SPP1), CD24 antigen (CD24), and
neuromedin B (NMB), were evaluated using quantitative RT-PCR
and in situ hybridization. In situ hybridization localized
gene expression for NMB, CD24, AKR1B1, and SPP1 in the
luminal epithelium, and confirmed the expression patterns
from RT-PCR analysis. The aberrant expression patterns of
endometrial AKR1B1, SPP1, CD24, and NMB 3-4 d after
premature estrogen exposure to pregnant gilts may be
involved with conceptus attachment failure to the uterine
surface epithelium and induction of endometrial responses
that disrupt the establishment of a viable
pregnancy.},
Language = {eng},
Doi = {10.1210/en.2007-0599},
Key = {fds174176}
}
@article{fds268704,
Author = {Cao, W and Li, W and Han, H and O'Leary-Moore, SK and Sulik, KK and Allan
Johnson, G and Liu, C},
Title = {Prenatal alcohol exposure reduces magnetic susceptibility
contrast and anisotropy in the white matter of mouse
brains.},
Journal = {Neuroimage},
Volume = {102 Pt 2},
Number = {0 2},
Pages = {748-755},
Year = {2014},
Month = {November},
ISSN = {1053-8119},
url = {http://dx.doi.org/10.1016/j.neuroimage.2014.08.035},
Abstract = {Prenatal alcohol exposure can result in long-term cognitive
and behavioral deficits. Fetal alcohol spectrum disorder
(FASD) refers to a range of permanent birth defects caused
by prenatal alcohol exposure, and is the most common
neurodevelopmental disorder in the US. Studies by autopsy
and conventional structural MRI indicate that the midline
structures of the brain are particularly vulnerable to
prenatal alcohol exposure. Diffusion tensor imaging (DTI)
has shown that abnormalities in brain white matter
especially the corpus callosum are very common in FASD.
Quantitative susceptibility mapping (QSM) is a novel
technique that measures tissue's magnetic property. Such
magnetic property is affected by tissue microstructure and
molecular composition including that of myelin in the white
matter. In this work, we studied three major white matter
fiber bundles of a mouse model of FASD and compared it to
control mice using both QSM and DTI. QSM revealed clear and
significant abnormalities in anterior commissure, corpus
callosum, and hippocampal commissure, which were likely due
to reduced myelination. Our data also suggested that QSM may
be even more sensitive than DTI for examining changes due to
prenatal alcohol exposure. Although this is a preclinical
study, the technique of QSM is readily translatable to human
brain.},
Doi = {10.1016/j.neuroimage.2014.08.035},
Key = {fds268704}
}
@article{fds367905,
Author = {Hornburg, KJ and Slosky, LM and Cofer, G and Cook, J and Qi, Y and Porkka,
F and Clark, NB and Pires, A and Petrella, JR and White, LE and Wetsel, WC and Barak, L and Caron, MG and Johnson, GA},
Title = {Prenatal heroin exposure alters brain morphology and
connectivity in adolescent mice.},
Journal = {NMR Biomed},
Volume = {36},
Number = {2},
Pages = {e4842},
Year = {2023},
Month = {February},
url = {http://dx.doi.org/10.1002/nbm.4842},
Abstract = {The United States is experiencing a dramatic increase in
maternal opioid misuse and, consequently, the number of
individuals exposed to opioids in utero. Prenatal opioid
exposure has both acute and long-lasting effects on health
and wellbeing. Effects on the brain, often identified at
school age, manifest as cognitive impairment, attention
deficit, and reduced scholastic achievement. The
neurobiological basis for these effects is poorly
understood. Here, we examine how in utero exposure to heroin
affects brain development into early adolescence in a mouse
model. Pregnant C57BL/6J mice received escalating doses of
heroin twice daily on gestational days 4-18. The brains of
offspring were assessed on postnatal day 28 using 9.4 T
diffusion MRI of postmortem specimens at 36 μm
resolution. Whole-brain volumes and the volumes of 166
bilateral regions were compared between heroin-exposed and
control offspring. We identified a reduction in whole-brain
volume in heroin-exposed offspring and heroin-associated
volume changes in 29 regions after standardizing for
whole-brain volume. Regions with bilaterally reduced
standardized volumes in heroin-exposed offspring relative to
controls include the ectorhinal and insular cortices.
Regions with bilaterally increased standardized volumes in
heroin-exposed offspring relative to controls include the
periaqueductal gray, septal region, striatum, and
hypothalamus. Leveraging microscopic resolution diffusion
tensor imaging and precise regional parcellation, we
generated whole-brain structural MRI diffusion connectomes.
Using a dimension reduction approach with multivariate
analysis of variance to assess group differences in the
connectome, we found that in utero heroin exposure altered
structure-based connectivity of the left septal region and
the region that acts as a hub for limbic regulatory actions.
Consistent with clinical evidence, our findings suggest that
prenatal opioid exposure may have effects on brain
morphology, connectivity, and, consequently, function that
persist into adolescence. This work expands our
understanding of the risks associated with opioid misuse
during pregnancy and identifies biomarkers that may
facilitate diagnosis and treatment.},
Doi = {10.1002/nbm.4842},
Key = {fds367905}
}
@article{fds174208,
Author = {TD Tillmanns and CA Falkner and DB Engle and JY Wan and RS Mannel and JL
Walker, GA Johnson and DS McMeekin and R Zuna and MA
Gold},
Title = {Preoperative predictors of positive margins after loop
electrosurgical excisional procedure-Cone.},
Journal = {Gynecologic oncology},
Volume = {100},
Number = {2},
Pages = {379-84},
Year = {2006},
Month = {February},
ISSN = {0090-8258},
url = {http://dx.doi.org/10.1016/j.ygyno.2005.09.015},
Keywords = {Adult • Cervical Intraepithelial Neoplasia •
Conization • Electrosurgery • Female • Humans
• Predictive Value of Tests • Preoperative Care
• Retrospective Studies • Uterine Cervical
Dysplasia • Uterine Cervical Neoplasms • methods*
• pathology* • surgery*},
Abstract = {OBJECTIVE: A LEEP-Cone may not be necessary for all patients
with traditional cone indications. This study defines
populations where a single pass technique with the LEEP is
appropriate. METHODS: We retrospectively reviewed patients
undergoing LEEP-Cone procedures performed at the University
of Oklahoma Health Science Center from February of 1994 to
July of 2002. Patients include those for LEEP-Cone with
traditional excisional indications and those who underwent
LEEP-Cone at the operating physician's discretion.
Statistical analysis was used to compare preoperative
factors with the resultant pathologic results. RESULTS: A
total of 248 women underwent LEEP-Cone. 50.0% (33/66) of the
patients with positive margins on the first pass had
dysplasia or worse (CIN I-III or CA) in the second pass (top
hat), compared to 6.6% (12/182) of the patients with a
negative first pass (P < 0.0001). Univariate analysis found
CIN III on histology and parity to be predictive of
dysplasia in the top hat and two-step discrepancy to predict
absence of dysplasia. On multivariate analysis, two-step
discrepancy and parity remained predictive. Age >35 was the
greatest percentile predictor of dysplasia in the top hat,
and 91.5% of women <21 had normal top hat pathology.
CONCLUSION: The retrospective data reported regarding
LEEP-Cones reveal increased parity to predict dysplasia in
the top hat and two-step discrepancy as a poor predictor of
dysplasia in the top hat. Women under 21 years of age should
have a single pass LEEP technique. The "top hat" is more
appropriate as parity and age increase.},
Language = {eng},
Doi = {10.1016/j.ygyno.2005.09.015},
Key = {fds174208}
}
@booklet{Burvill95,
Author = {P. W. Burvill and G. A. Johnson and K. D. Jamrozik and C. S.
Anderson and E. G. Stewartwynne and T. M. H.
Chakera},
Title = {Prevalence of depression after stroke - the perth community
stroke study},
Journal = {British Journal Of Psychiatry},
Volume = {166},
Pages = {320 -- 327},
Year = {1995},
Month = {March},
Key = {Burvill95}
}
@article{fds174205,
Author = {PW Burvill and GA Johnson and KD Jamrozik and CS Anderson and EG
Stewart-Wynne, TM Chakera},
Title = {Prevalence of depression after stroke: the Perth Community
Stroke Study.},
Journal = {The British journal of psychiatry : the journal of mental
science},
Volume = {166},
Number = {3},
Pages = {320-7},
Year = {1995},
Month = {March},
ISSN = {0007-1250},
Keywords = {Adaptation, Psychological • Adult • Aged •
Aged, 80 and over • Cerebrovascular Disorders •
Cohort Studies • Cross-Sectional Studies •
Delirium, Dementia, Amnestic, Cognitive Disorders •
Depressive Disorder • Female • Follow-Up Studies
• Humans • Incidence • Male • Middle
Aged • Recurrence • Sick Role • Western
Australia • diagnosis • epidemiology •
epidemiology* • psychology},
Abstract = {BACKGROUND: The Perth Community Stroke Study (PCSS) was a
population-based study of the incidence, cause, and outcome
of acute stroke. METHOD: Subjects from the study were
assessed initially, by examination and interview, and at
four- and 12-month follow-ups to determine differences in
prevalence of depression between the sexes and between
patients with first-ever and recurrent strokes. RESULTS: The
prevalence of depressive illness four months after stroke in
294 patients from the PCSS was 23% (18-28%), 15% (11-19%)
major depression and 8% (5-11%) minor depression. There were
no significant differences between the sexes or between
patients with first-ever and recurrent strokes. With a
non-hierarchic approach to diagnosis of those with
depression, 26% of men and 39% of women had an associated
anxiety disorder, mainly agoraphobia. Nine per cent of male
and 13% of female patients interviewed had evidence of
depression at the time of the stroke. Twelve months after
stroke 56% of the men were still depressed (40% major and
16% minor), as were 30% of the women (12% major and 18%
minor). CONCLUSION: The prevalence of depression after
stroke was comparable with that reported from other studies,
and considerably less than that reported from in-patient and
rehabilitation units.},
Language = {eng},
Key = {fds174205}
}
@article{fds174095,
Author = {TD Chinevere and CK Murray and E Grant Jr and GA Johnson and F Duelm and DR
Hospenthal},
Title = {Prevalence of glucose-6-phosphate dehydrogenase deficiency
in U.S. Army personnel.},
Journal = {Military medicine},
Volume = {171},
Number = {9},
Pages = {905-7},
Year = {2006},
Month = {September},
ISSN = {0026-4075},
Keywords = {Adult • Endemic Diseases • Female •
Glucosephosphate Dehydrogenase • Glucosephosphate
Dehydrogenase Deficiency • Humans • Malaria •
Male • Mass Screening • Military Medicine* •
Military Personnel • Occupational Health* •
Prevalence • Primaquine • Retrospective Studies
• Risk Assessment • Risk Factors • United
States • adverse effects • analysis • blood*
• diagnosis • drug therapy • epidemiology
• epidemiology* • statistics & numerical data*
• therapeutic use},
Abstract = {The U.S. Army recently mandated that soldiers undergo
glucose-6-phosphate dehydrogenase (G6PD) testing before
deployment to malarious regions. We retrospectively
characterize the presence and degree of G6PD deficiency in
U.S. military personnel by sex, self-reported ethnicity, and
World Health Organization deficiency classification through
test results obtained October 1, 2004 through January 17,
2005. Data were available for 63,302 (54,874 males and 8,428
females) subjects; 2.5% of males and 1.6% of females were
deficient, with most having only moderate enzyme deficiency.
African American males (12.2%) and females (4.1%), along
with Asian males (4.3%), had the highest rates of G6PD
deficiency. Most males were found to have class III variants
while most females were class IV variants. The most severely
deficient were Asian males (class II). These results suggest
that universal screening for G6PD deficiency is clinically
warranted, and particularly essential for those male service
members who self-report ethnicity as African American,
Asian, or Hispanic.},
Language = {eng},
Key = {fds174095}
}
@article{fds132752,
Author = {GA Johnson and N Danieley and CE Ravin},
Title = {Processing alternatives for digital chest
imaging.},
Journal = {Radiologic clinics of North America, UNITED
STATES},
Volume = {23},
Number = {2},
Pages = {335-40},
Year = {1985},
Month = {June},
ISSN = {0033-8389},
Keywords = {Humans • Radiographic Image Enhancement •
Radiography, Thoracic • methods •
methods*},
Abstract = {Radiographic imaging of the chest remains one of the most
important and most challenging problems in radiology. The
wide range of information that results from the great
variation of radiation behind the lungs compared with that
behind the mediastinum creates a very difficult imaging
problem. The introduction and continued investigation of
digital techniques have presented a potential solution to
this problem. In this article, the authors describe the
image-processing techniques of histogram equalization and
adaptive filtration in digital chest imaging.},
Key = {fds132752}
}
@booklet{Johnson85a,
Author = {Johnson, GA and Danieley, N and Ravin, CE},
Title = {Processing alternatives for digital chest
imaging.},
Journal = {Radiol Clin North Am},
Volume = {23},
Number = {2},
Pages = {335-340},
Year = {1985},
Month = {June},
ISSN = {0033-8389},
url = {http://www.ncbi.nlm.nih.gov/pubmed/3991890},
Abstract = {Radiographic imaging of the chest remains one of the most
important and most challenging problems in radiology. The
wide range of information that results from the great
variation of radiation behind the lungs compared with that
behind the mediastinum creates a very difficult imaging
problem. The introduction and continued investigation of
digital techniques have presented a potential solution to
this problem. In this article, the authors describe the
image-processing techniques of histogram equalization and
adaptive filtration in digital chest imaging.},
Key = {Johnson85a}
}
@booklet{Asselin99,
Author = {E. Asselin and G. A. Johnson and T. E. Spencer and W. B.
Fuller},
Title = {Progesterone and interferon-tau regulate monocyte
chemotactic protein-1 (MCP-1) in the ovine
uterus.},
Journal = {Biology Of Reproduction},
Volume = {60},
Pages = {95 -- 95},
Year = {1999},
Key = {Asselin99}
}
@article{fds174256,
Author = {TE Spencer and GA Johnson and RC Burghardt and FW
Bazer},
Title = {Progesterone and placental hormone actions on the uterus:
insights from domestic animals.},
Journal = {Biology of reproduction},
Volume = {71},
Number = {1},
Pages = {2-10},
Year = {2004},
Month = {July},
ISSN = {0006-3363},
url = {http://dx.doi.org/10.1095/biolreprod.103.024133},
Keywords = {Animals • Animals, Domestic • Female •
Placental Hormones • Pregnancy • Pregnancy, Animal
• Progesterone • Sheep • Swine • Uterus
• physiology • physiology*},
Abstract = {Progesterone is unequivocally required for maternal support
of conceptus (embryo/fetus and associated extraembryonic
membranes) survival and development. In cyclic sheep,
progesterone is paradoxically involved in suppressing and
then initiating development of the endometrial luteolytic
mechanism. In cyclic and pregnant sheep, progesterone
negatively autoregulates progesterone receptor (PR) gene
expression in the endometrial luminal (LE) and superficial
glandular epithelium (GE). In cyclic sheep, PR loss is
closely followed by increases in epithelial estrogen
receptor (ERalpha) and then oxytocin receptor (OTR),
allowing oxytocin to induce uterine release of luteolytic
prostaglandin F2alpha pulses. In pregnant sheep, the
conceptus produces interferon tau (IFNtau) that acts on the
endometrium to inhibit transcription of the ERalpha gene and
thus development of the endometrial luteolytic mechanism.
After Day 13 of pregnancy, the endometrial epithelia do not
express the PR, whereas the stroma and myometrium remain PR
positive. The absence of PR in the endometrial GE is
required for onset of differentiated function of the glands
during pregnancy. The sequential, overlapping actions of
progesterone, IFNtau, placental lactogen (PL), and growth
hormone (GH) comprise a hormonal servomechanism that
regulates endometrial gland morphogenesis and terminal
differentiated function during gestation. In pigs, estrogen,
the pregnancy-recognition signal, increases fibroblast
growth factor 7 (FGF-7) expression in the endometrial LE
that, in turn, stimulates proliferation and differentiated
functions of the trophectoderm, which expresses the receptor
for FGF-7. Strategic manipulation of these physiological
mechanisms may offer therapeutic schemes to improve uterine
capacity, conceptus survival, and reproductive health of
domestic animals and humans.},
Language = {eng},
Doi = {10.1095/biolreprod.103.024133},
Key = {fds174256}
}
@article{fds174106,
Author = {KA Dunlap and DW Erikson and RC Burghardt and FJ White and KM Reed and JL
Farmer, TE Spencer and RR Magness and FW Bazer and KJ Bayless and GA
Johnson},
Title = {Progesterone and placentation increase secreted
phosphoprotein one (SPP1 or osteopontin) in uterine glands
and stroma for histotrophic and hematotrophic support of
ovine pregnancy.},
Journal = {Biology of reproduction},
Volume = {79},
Number = {5},
Pages = {983-90},
Year = {2008},
Month = {November},
ISSN = {0006-3363},
url = {http://dx.doi.org/10.1095/biolreprod.108.071068},
Keywords = {Animals • Embryonic Development* • Female •
Osteopontin • Pregnancy • Pregnancy, Animal •
Progesterone • Sheep • Uterus • metabolism*
• physiology*},
Abstract = {Secreted phosphoprotein one (SPP1, osteopontin) may regulate
conceptus implantation and placentation. We investigated
effects of progesterone (P(4)) and the conceptus on
expression and localization of SPP1 in the ovine uterus.
Steady-state levels of SPP1 mRNA in the endometrium of
unilaterally pregnant ewes did not differ significantly
between nongravid and gravid horns within their respective
days of pregnancy; however, levels did increase as pregnancy
progressed. SPP1 mRNA was detectable in the glandular
epithelium (GE) of both nongravid and gravid horns via in
situ hybridization. SPP1 protein was localized to the apical
surface of the luminal epithelium of both nongravid and
gravid uterine horns. Gravid horns exhibited extensive
stromal SPP1 on Days 40 through 120, whereas SPP1 was
markedly lower in the stroma of nongravid uterine horns
through Day 80 of pregnancy. By Day 120, stromal expression
of SPP1 between nongravid and gravid horns was similar.
Long-term P(4) treatment of ovariectomized ewes induced SPP1
in the uterine stroma and GE. A bioactive 45-kDa SPP1
fragment was purified from uterine secretions and promoted
ovine trophectoderm cell attachment in vitro. Interestingly,
increased stromal cell expression of SPP1 was positively
associated with vascularization as assessed by von
Willebrand factor staining. Finally, ovine uterine artery
endothelial cells produced SPP1 during outgrowth into
three-dimensional collagen matrices in an in vitro model
system that recapitulates angiogenesis. Collectively, P(4)
induces and the conceptus further stimulates SPP1 in uterine
GE and stroma, where SPP1 likely influences histotrophic and
hematotrophic support of conceptus development.},
Language = {eng},
Doi = {10.1095/biolreprod.108.071068},
Key = {fds174106}
}
@booklet{Johnson99e,
Author = {G. A. Johnson and T. E. Spencer and R. C. Burghardt and F.
W. Bazer},
Title = {Progesterone induces osteopontin expression in ovine
endometrial glands.},
Journal = {Biology Of Reproduction},
Volume = {60},
Pages = {210 -- 211},
Year = {1999},
Key = {Johnson99e}
}
@booklet{Johnson00b,
Author = {G. A. Johnson and T. E. Spencer and R. C. Burghardt and K.
M. Taylor and C. A. Gray and F. W. Bazer},
Title = {Progesterone modulation of osteopontin gene expression in
the ovine uterus},
Journal = {Biology Of Reproduction},
Volume = {62},
Number = {5},
Pages = {1315 -- 1321},
Year = {2000},
Month = {May},
Key = {Johnson00b}
}
@article{fds174108,
Author = {GA Johnson and TE Spencer and RC Burghardt and KM Taylor and CA Gray and FW
Bazer},
Title = {Progesterone modulation of osteopontin gene expression in
the ovine uterus.},
Journal = {Biology of reproduction},
Volume = {62},
Number = {5},
Pages = {1315-21},
Year = {2000},
Month = {May},
ISSN = {0006-3363},
Keywords = {Animals • Blotting, Western • Endometrium •
Epithelial Cells • Female • Fluorescent Antibody
Technique • Gene Expression • In Situ
Hybridization • Interferons • Osteopontin •
Ovariectomy • Progesterone • Receptors,
Progesterone • Recombinant Proteins • Sheep •
Sialoglycoproteins • Uterus • antagonists &
inhibitors • cytology • drug effects •
genetics* • metabolism • metabolism* •
pharmacology • physiology • physiology*},
Abstract = {Osteopontin (OPN) is an acidic phosphorylated glycoprotein
component of the extracellular matrix that binds to
integrins at the cell surface to promote cell-cell
attachment and cell spreading. This matrix constituent is a
ligand that could potentially bind integrins on
trophectoderm and endometrium to facilitate superficial
implantation and placentation. OPN mRNA increases in the
endometrial glandular epithelium (GE) of early-pregnant
ewes, and OPN protein is secreted into the uterine lumen.
Therefore, progesterone and/or interferon-tau (IFNtau) may
regulate OPN expression in the uterine GE. Cyclic ewes were
ovariectomized and fitted with intrauterine (i. u.)
catheters on Day 5 and treated daily with steroids (i.m.)
and protein (i.u.) as follows: 1) progesterone (P, Days
5-24) and control serum proteins (CX, Days 11-24); 2) P and
ZK 136.317 (ZK; progesterone receptor [PR] antagonist, Days
11-24) and CX proteins; 3) P and recombinant ovine IFNtau
(roIFNtau, Days 11-24); or 4) P and ZK and roIFNtau. All
ewes were hysterectomized on Day 25. Progesterone induced
the expression of endometrial OPN mRNA in the GE and
increased secretion of a 45-kDa OPN protein from endometrial
explants maintained in culture for 24 h. Administration of
ZK ablated progesterone effects. Intrauterine infusion of
roIFNtau did not affect OPN gene expression or secretion in
any of the steroid treatments. Interestingly, OPN
mRNA-positive GE cells lacked detectable PR expression,
although PR were detected in the stroma. Results indicate
that progesterone regulates OPN expression in GE through a
complex mechanism that includes PR down-regulation, and we
suggest the possible involvement of a progesterone-induced
stromal cell-derived growth factor(s) that acts as a
progestamedin.},
Language = {eng},
Key = {fds174108}
}
@booklet{Qiu97a,
Author = {Qiu, H and Hedlund, LW and Gewalt, SL and Benveniste, H and Bare, TM and Johnson, GA},
Title = {Progression of a focal ischemic lesion in rat brain during
treatment with a novel glycine/NMDA antagonist: an in vivo
three-dimensional diffusion-weighted MR microscopy
study.},
Journal = {J Magn Reson Imaging},
Volume = {7},
Number = {4},
Pages = {739-744},
Year = {1997},
url = {http://dx.doi.org/10.1002/jmri.1880070421},
Abstract = {Stroke was induced in two groups of anesthetized rats by
occlusion of the middle cerebral artery (MCA) and
ipsilateral common carotid artery. Group 1 (control)
received vehicle and group 2 received the glycine
N-methyl-D-aspartate (NMDA) antagonist ZD9379. Stroke volume
was assessed by three-dimensional diffusion-weighted MR
microscopy at 2.5 and 6 hours of MCA occlusion. At 2.5
hours, stroke volumes were identical in the two groups. At 6
hours, stroke volumes had increased by 15% in the control
group; in contrast, the treated group showed a 40% reduced
stroke volume. Conclusions from this in vivo study were as
follows: (a) our technique allows more efficient and
accurate measurement of stroke volume with an improvement in
resolution over a previous method; (b) the ability to
measure stroke volume at multiple time points shows volume
change and assessment of time dependency of drug treatment;
(c) at 6 hours, the glycine antagonist ZD9379 reduced stroke
volume by 40%.},
Doi = {10.1002/jmri.1880070421},
Key = {Qiu97a}
}
@article{fds132877,
Author = {H Qiu and LW Hedlund and SL Gewalt and H Benveniste and TM Bare and GA
Johnson},
Title = {Progression of a focal ischemic lesion in rat brain during
treatment with a novel glycine/NMDA antagonist: an in vivo
three-dimensional diffusion-weighted MR microscopy
study.},
Journal = {Journal of magnetic resonance imaging : JMRI, UNITED
STATES},
Volume = {7},
Number = {4},
Pages = {739-44},
ISSN = {1053-1807},
Keywords = {Animals • Brain • Brain Ischemia • Female
• Image Processing, Computer-Assisted • Magnetic
Resonance Imaging • Pyridazines • Quinolines
• Rats • Rats, Inbred F344 • Receptors,
Glycine • Time Factors • antagonists & inhibitors*
• drug therapy* • methods • methods* •
pathology • pathology* • therapeutic
use*},
Abstract = {Stroke was induced in two groups of anesthetized rats by
occlusion of the middle cerebral artery (MCA) and
ipsilateral common carotid artery. Group 1 (control)
received vehicle and group 2 received the glycine
N-methyl-D-aspartate (NMDA) antagonist ZD9379. Stroke volume
was assessed by three-dimensional diffusion-weighted MR
microscopy at 2.5 and 6 hours of MCA occlusion. At 2.5
hours, stroke volumes were identical in the two groups. At 6
hours, stroke volumes had increased by 15% in the control
group; in contrast, the treated group showed a 40% reduced
stroke volume. Conclusions from this in vivo study were as
follows: (a) our technique allows more efficient and
accurate measurement of stroke volume with an improvement in
resolution over a previous method; (b) the ability to
measure stroke volume at multiple time points shows volume
change and assessment of time dependency of drug treatment;
(c) at 6 hours, the glycine antagonist ZD9379 reduced stroke
volume by 40%.},
Key = {fds132877}
}
@booklet{Stewart00a,
Author = {M. D. Stewart and G. A. Johnson and C. A. Gray and R. C.
Burghardt and L. A. Schuler and M. M. Joyce and F. W. Bazer and T. E. Spencer},
Title = {Prolactin receptor and uterine milk protein expression in
the ovine endometrium during the estrous cycle and
pregnancy},
Journal = {Biology Of Reproduction},
Volume = {62},
Number = {6},
Pages = {1779 -- 1789},
Year = {2000},
Month = {June},
Key = {Stewart00a}
}
@article{fds174216,
Author = {MD Stewart and GA Johnson and CA Gray and RC Burghardt and LA Schuler and MM Joyce and FW Bazer and TE Spencer},
Title = {Prolactin receptor and uterine milk protein expression in
the ovine endometrium during the estrous cycle and
pregnancy.},
Journal = {Biology of reproduction},
Volume = {62},
Number = {6},
Pages = {1779-89},
Year = {2000},
Month = {June},
ISSN = {0006-3363},
Keywords = {Animals • Endometrium • Estrus • Female
• Fluorescent Antibody Technique • Gene
Expression* • Glycoproteins • Immunohistochemistry
• In Situ Hybridization • Pregnancy • RNA,
Messenger • Receptors, Prolactin • Reverse
Transcriptase Polymerase Chain Reaction • Serpins*
• Sheep* • analysis • genetics* •
metabolism* • physiology*},
Abstract = {Lactogenic hormones regulate epithelial proliferation,
differentiation, and function in a variety of
epitheliomesenchymal organs. During pregnancy, the ovine
uterus is a potential site for endocrine and paracrine
actions of lactogenic hormones in the form of pituitary
prolactin (PRL) and placental lactogen (PL). These studies
determined temporal and spatial alterations in PRL receptor
(PRL-R) and expression of uterine milk proteins (UTMP), a
marker of endometrial secretory activity, in the ovine
endometrium during the estrous cycle and pregnancy.
Slot-blot hybridization analysis indicated that steady-state
levels of endometrial PRL-R mRNA increased during pregnancy.
In situ hybridization and immunohistochemical analyses
indicated that PRL-R mRNA and protein were exclusively
expressed in the endometrial glandular epithelium (GE). No
PRL-R mRNA expression was detected in luminal epithelium,
stroma, myometrium, or conceptus trophectoderm. Reverse
transcription-polymerase chain reaction analyses determined
that the endometrial GE expressed both long and short
alternative splice forms of the ovine PRL-R gene. Slot-blot
hybridization analysis indicated that steady-state levels of
intercaruncular endometrial UTMP mRNA increased about 3-fold
between Days 20 and 60, increased another 3-fold between
Days 60 and 80, and then declined slightly to Day 120. In
pregnant ewes, UTMP mRNA expression was restricted to the
endometrial GE in the stratum spongiosum (sGE), increased
substantially between Days 15 and 17, and, between Days 17
to 50 of gestation, was markedly higher in upper than lower
sGE. After Day 50, hyperplasia of the sGE was accompanied by
increased UTMP mRNA expression by all sGE. Collectively,
results indicate that 1) endometrial sGE is a primary target
for actions of lactogenic hormones and 2) UTMP mRNA
expression is correlated with PL production by the
trophectoderm and state of sGE differentiation during
pregnancy. It is proposed that activation of PRL-R signal
transduction pathways by PRL and PL plays a major role in
endometrial GE remodeling and differentiated function during
pregnancy in support of conceptus growth and
development.},
Language = {eng},
Key = {fds174216}
}
@booklet{Stewart99,
Author = {M. D. Stewart and G. A. Johnson and A. G. Stagg and R. C.
Burghardt and L. A. Schuler and F. W. Bazer and T. E.
Spencer},
Title = {Prolactin receptor expression in the ovine
uterus.},
Journal = {Biology Of Reproduction},
Volume = {60},
Pages = {150 -- 150},
Year = {1999},
Key = {Stewart99}
}
@article{fds180931,
Author = {G Wu and FW Bazer and RC Burghardt and GA Johnson and SW Kim and DA Knabe and P Li and X Li, JR McKnight and MC Satterfield and TE
Spencer},
Title = {Proline and hydroxyproline metabolism: implications for
animal and human nutrition.},
Journal = {Amino acids},
Year = {2010},
Month = {August},
ISSN = {1438-2199},
url = {http://dx.doi.org/10.1007/s00726-010-0715-z},
Abstract = {Proline plays important roles in protein synthesis and
structure, metabolism (particularly the synthesis of
arginine, polyamines, and glutamate via pyrroline-5-carboxylate),
and nutrition, as well as wound healing, antioxidative
reactions, and immune responses. On a per-gram basis,
proline plus hydroxyproline are most abundant in collagen
and milk proteins, and requirements of proline for
whole-body protein synthesis are the greatest among all
amino acids. Therefore, physiological needs for proline are
particularly high during the life cycle. While most mammals
(including humans and pigs) can synthesize proline from
arginine and glutamine/glutamate, rates of endogenous
synthesis are inadequate for neonates, birds, and fish.
Thus, work with young pigs (a widely used animal model for
studying infant nutrition) has shown that supplementing 0.0,
0.35, 0.7, 1.05, 1.4, and 2.1% proline to a proline-free
chemically defined diet containing 0.48% arginine and 2%
glutamate dose dependently improved daily growth rate and
feed efficiency while reducing concentrations of urea in
plasma. Additionally, maximal growth performance of chickens
depended on at least 0.8% proline in the diet. Likewise,
dietary supplementation with 0.07, 0.14, and 0.28%
hydroxyproline (a metabolite of proline) to a plant
protein-based diet enhanced weight gains of salmon. Based on
its regulatory roles in cellular biochemistry, proline can
be considered as a functional amino acid for mammalian,
avian, and aquatic species. Further research is warranted to
develop effective strategies of dietary supplementation with
proline or hydroxyproline to benefit health, growth, and
development of animals and humans.},
Language = {ENG},
Doi = {10.1007/s00726-010-0715-z},
Key = {fds180931}
}
@article{fds174207,
Author = {G Wu and FW Bazer and S Datta and GA Johnson and P Li and MC Satterfield and TE Spencer},
Title = {Proline metabolism in the conceptus: implications for fetal
growth and development.},
Journal = {Amino acids},
Volume = {35},
Number = {4},
Pages = {691-702},
Year = {2008},
Month = {November},
ISSN = {1438-2199},
url = {http://dx.doi.org/10.1007/s00726-008-0052-7},
Keywords = {Amino Acids • Animals • Embryo, Mammalian •
Female • Fetal Development* • Humans •
Models, Biological • Placenta • Polyamines •
Pregnancy • Pregnancy, Animal • Proline •
Sheep • Swine • metabolism •
metabolism*},
Abstract = {Although there are published studies of proline biochemistry
and nutrition in cultured cells and postnatal animals,
little is known about proline metabolism and function in the
conceptus (embryo/fetus, associated placental membranes, and
fetal fluids). Because of the invasive nature of biochemical
research on placental and fetal growth, animal models are
often used to test hypotheses of biological importance.
Recent evidence from studies with pigs and sheep shows that
proline is a major substrate for polyamine synthesis via
proline oxidase, ornithine aminotransferase, and ornithine
decarboxylase in placentae. Both porcine and ovine placentae
have a high capacity for proline catabolism and polyamine
production. In addition, allantoic and amniotic fluids
contain enzymes to convert proline into ornithine, which is
delivered through the circulation to placental tissues.
There is exquisite metabolic coordination among integrated
pathways that support highest rates of polyamine synthesis
and concentrations in placentae during early gestation when
placental growth is most rapid. Interestingly, reduced
placental and fetal growth are associated with reductions in
placental proline transport, proline oxidase activity, and
concentrations of polyamines in gestating dams with either
naturally occurring or malnutrition-induced growth
retardation. Conversely, increasing proline availability in
maternal plasma through nutritional or pharmacological
modulation in pigs and sheep enhances concentrations of
proline and polyamines in placentae and fetal fluids, as
well as fetal growth. These novel findings suggest an
important role for proline in conceptus metabolism, growth
and development, as well as a potential treatment for
intrauterine growth restriction, which is a significant
problem in both human medicine and animal
agriculture.},
Language = {eng},
Doi = {10.1007/s00726-008-0052-7},
Key = {fds174207}
}
@article{fds174186,
Author = {GA Johnson},
Title = {Psychological sequelae in stroke patients.},
Journal = {Australian family physician},
Volume = {20},
Number = {11},
Pages = {1605-7, 1610-1},
Year = {1991},
Month = {November},
ISSN = {0300-8495},
Keywords = {Anxiety Disorders • Cerebrovascular Disorders •
Dementia • Depression • Humans • etiology
• psychology* • therapy},
Abstract = {The psychological sequelae of depression, anxiety and
dementia are discussed as they affect the stroke sufferer.
The author discusses the management options available to
deal with these problems.},
Language = {eng},
Key = {fds174186}
}
@article{fds268858,
Author = {Mistry, NN and Pollaro, J and Song, J and De Lin and M and Johnson,
GA},
Title = {Pulmonary perfusion imaging in the rodent lung using dynamic
contrast-enhanced MRI.},
Journal = {Magn Reson Med},
Volume = {59},
Number = {2},
Pages = {289-297},
Year = {2008},
Month = {February},
ISSN = {0740-3194},
url = {http://www.ncbi.nlm.nih.gov/pubmed/18228577},
Keywords = {Animals • Contrast Media • Female •
Gadolinium DTPA • Image Processing, Computer-Assisted
• Lung • Magnetic Resonance Imaging •
Phantoms, Imaging • Rats • Rats, Inbred F344
• blood supply* • methods* •
pharmacokinetics*},
Abstract = {With the development of various models of pulmonary disease,
there is tremendous interest in quantitative regional
assessment of pulmonary function. While ventilation imaging
has been addressed to a certain extent, perfusion imaging
for small animals has not kept pace. In humans and large
animals perfusion can be assessed using dynamic
contrast-enhanced (DCE) MRI with a single bolus injection of
a gadolinium (Gd)-based contrast agent. But the method
developed for the clinic cannot be translated directly to
image the rodent due to the combined requirements of higher
spatial and temporal resolution. This work describes a novel
image acquisition technique staggered over multiple,
repeatable bolus injections of contrast agent using an
automated microinjector, synchronized with image acquisition
to achieve dynamic first-pass contrast enhancement in the
rat lung. This allows dynamic first-pass imaging that can be
used to quantify pulmonary perfusion. Further improvements
are made in the spatial and temporal resolution by combining
the multiple injection acquisition method with Interleaved
Radial Imaging and "Sliding window-keyhole" reconstruction
(IRIS). The results demonstrate a simultaneous increase in
spatial resolution (<200 mum) and temporal resolution (<200
ms) over previous methods, with a limited loss in
signal-to-noise-ratio.},
Doi = {10.1002/mrm.21353},
Key = {fds268858}
}
@article{fds268841,
Author = {MacKenzie-Graham, A and Tiwari-Woodruff, SK and Sharma, G and Aguilar, C and Vo, KT and Strickland, LV and Morales, L and Fubara, B and Martin, M and Jacobs, RE and Johnson, GA and Toga, AW and Voskuhl,
RR},
Title = {Purkinje cell loss in experimental autoimmune
encephalomyelitis.},
Journal = {Neuroimage},
Volume = {48},
Number = {4},
Pages = {637-651},
Year = {2009},
Month = {December},
ISSN = {1053-8119},
url = {http://dx.doi.org/10.1016/j.neuroimage.2009.06.073},
Abstract = {Gray matter atrophy observed by brain MRI is an important
correlate to clinical disability and disease duration in
multiple sclerosis. The objective of this study was to link
brain atrophy visualized by neuroimaging to its underlying
neuropathology using the MS model, experimental autoimmune
encephalomyelitis (EAE). Volumetric changes in brains of EAE
mice, as well as matched healthy normal controls, were
quantified by collecting post-mortem high-resolution
T2-weighted magnetic resonance microscopy and actively
stained magnetic resonance histology images. Anatomical
delineations demonstrated a significant decrease in the
volume of the whole cerebellum, cerebellar cortex, and
molecular layer of the cerebellar cortex in EAE as compared
to normal controls. The pro-apoptotic marker caspase-3 was
detected in Purkinje cells and a significant decrease in
Purkinje cell number was found in EAE. Cross modality and
temporal correlations revealed a significant association
between Purkinje cell loss on neuropathology and atrophy of
the molecular layer of the cerebellar cortex by
neuroimaging. These results demonstrate the power of using
combined population atlasing and neuropathology approaches
to discern novel insights underlying gray matter atrophy in
animal models of neurodegenerative disease.},
Doi = {10.1016/j.neuroimage.2009.06.073},
Key = {fds268841}
}
@booklet{Grist88,
Author = {T. M. Grist and J. S. Hyde and A. Jesmanowicz and G. A.
Johnson},
Title = {Quadrature detection local coils for mri and
mrs},
Journal = {Investigative Radiology},
Volume = {23},
Number = {9},
Pages = {S21 -- S21},
Year = {1988},
Month = {September},
Key = {Grist88}
}
@article{fds357201,
Author = {Sills, RC and Johnson, GA and Anderson, RJ and Johnson, CL and Staup, M and Brown, DL and Churchill, SR and Kurtz, DM and Cushman, JD and Waidyanatha, S and Robinson, VG and Cesta, MF and Andrews, DMK and Behl,
M and Shockley, KR and Little, PB},
Title = {Qualitative and Quantitative Neuropathology Approaches Using
Magnetic Resonance Microscopy (Diffusion Tensor Imaging) and
Stereology in a Hexachlorophene Model of Myelinopathy in
Sprague-Dawley Rats.},
Journal = {Toxicol Pathol},
Volume = {48},
Number = {8},
Pages = {965-980},
Year = {2020},
Month = {December},
url = {http://dx.doi.org/10.1177/0192623320968210},
Abstract = {It is well established that hexachlorophene, which is used
as an antibacterial agent, causes intramyelinic edema in
humans and animal models. The hexachlorophene myelinopathy
model, in which male Sprague-Dawley rats received 25 to 30
mg/kg hexachlorophene by gavage for up to 5 days, provided
an opportunity to compare traditional neuropathology
evaluations with magnetic resonance microscopy (MRM)
findings. In addition, stereology assessments of 3
neuroanatomical sites were compared to quantitative
measurements of similar structures by MRM. There were
positive correlations between hematoxylin and eosin and
luxol fast blue stains and MRM for identifying intramyelinic
edema in the cingulum of corpus callosum, optic chiasm,
anterior commissure (aca), lateral olfactory tracts,
pyramidal tracts (py), and white matter tracts in the
cerebellum. Stereology assessments were focused on the aca,
longitudinal fasciculus of the pons, and py and demonstrated
differences between control and treated rats, as was
observed using MRM. The added value of MRM assessments was
the ability to acquire qualitative 3-dimensional (3-D)
images and obtain quantitative measurements of intramyelinic
edema in 26 neuroanatomical sites in the intact brain. Also,
diffusion tensor imaging (fractional anisotropy [FA])
indicated that there were changes in the cytoarchitecture of
the white matter as detected by decreases in the FA in the
treated compared to the control rats. This study
demonstrates creative strategies that are possible using
qualitative and quantitative assessments of potential white
matter neurotoxicants in nonclinical toxicity studies. Our
results lead us to the conclusion that volumetric analysis
by MRM and stereology adds significant value to the standard
2-D microscopic evaluations.},
Doi = {10.1177/0192623320968210},
Key = {fds357201}
}
@booklet{Chotas96,
Author = {Chotas, HG and Floyd, CE and Johnson, GA and Ravin,
CE},
Title = {Quality control phantom for digital chest
radiography},
Journal = {RADIOLOGY},
Volume = {201},
Pages = {685-685},
Publisher = {RADIOLOGICAL SOC NORTH AMER},
Year = {1996},
Month = {November},
ISSN = {0033-8419},
url = {http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:A1996VP84300732&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=47d3190e77e5a3a53558812f597b0b92},
Key = {Chotas96}
}
@article{fds132883,
Author = {HG Chotas and CE Floyd and GA Johnson and CE Ravin},
Title = {Quality control phantom for digital chest
radiography.},
Journal = {Radiology, UNITED STATES},
Volume = {202},
Number = {1},
Pages = {111-6},
Year = {1997},
Month = {January},
ISSN = {0033-8419},
Keywords = {Humans • Phantoms, Imaging* • Quality Control
• Radiographic Image Enhancement • Radiography,
Thoracic • standards*},
Abstract = {PURPOSE: To develop and test a chest phantom for routine
quality control testing of digital radiography systems.
MATERIALS AND METHODS: The phantom was constructed from
sheets of copper, aluminum, and acrylic, which were cut and
arranged to yield a radiographic projection resembling that
of a human thorax. Regional test objects allowed
quantitative assessment of optical density, contrast detail,
and spatial resolution. Validation tests were performed to
assess image stability in a stable imaging environment and
sensitivity to changes in image quality when they occur.
RESULTS: The phantom yielded consistent pseudoclinical
images when used in a routine quality control program and
facilitated detection of simulated problems that were
induced in imaging system performance. CONCLUSION: The chest
phantom enables quantitative, full-system testing of digital
radiography system as they are used clinically for chest
radiography.},
Key = {fds132883}
}
@booklet{Chotas97,
Author = {Chotas, HG and Floyd, CE and Johnson, GA and Ravin,
CE},
Title = {Quality control phantom for digital chest
radiography.},
Journal = {Radiology},
Volume = {202},
Number = {1},
Pages = {111-116},
Year = {1997},
Month = {January},
ISSN = {0033-8419},
url = {http://www.ncbi.nlm.nih.gov/pubmed/8988199},
Abstract = {PURPOSE: To develop and test a chest phantom for routine
quality control testing of digital radiography systems.
MATERIALS AND METHODS: The phantom was constructed from
sheets of copper, aluminum, and acrylic, which were cut and
arranged to yield a radiographic projection resembling that
of a human thorax. Regional test objects allowed
quantitative assessment of optical density, contrast detail,
and spatial resolution. Validation tests were performed to
assess image stability in a stable imaging environment and
sensitivity to changes in image quality when they occur.
RESULTS: The phantom yielded consistent pseudoclinical
images when used in a routine quality control program and
facilitated detection of simulated problems that were
induced in imaging system performance. CONCLUSION: The chest
phantom enables quantitative, full-system testing of digital
radiography system as they are used clinically for chest
radiography.},
Doi = {10.1148/radiology.202.1.8988199},
Key = {Chotas97}
}
@article{fds268834,
Author = {Mistry, NN and Thomas, A and Kaushik, SS and Johnson, GA and Driehuys,
B},
Title = {Quantitative analysis of hyperpolarized 3He ventilation
changes in mice challenged with methacholine.},
Journal = {Magn Reson Med},
Volume = {63},
Number = {3},
Pages = {658-666},
Year = {2010},
Month = {March},
ISSN = {1522-2594},
url = {http://www.ncbi.nlm.nih.gov/pubmed/20187176},
Keywords = {Administration, Inhalation • Algorithms* • Animals
• Asthma • Contrast Media • Helium •
Image Enhancement • Isotopes • Magnetic Resonance
Imaging • Methacholine Chloride • Mice •
Mice, Inbred C57BL • Reproducibility of Results •
Sensitivity and Specificity • administration & dosage
• diagnosis* • diagnostic use • diagnostic
use* • methods*},
Abstract = {The capability to use high-resolution (3)He MRI to depict
regional ventilation changes and airway narrowing in mice
challenged with methacholine (MCh) offers the opportunity to
gain new insights into the study of asthma. However, to
fully exploit the value of this novel technique, it is
important to move beyond visual inspection of the images
toward automated and quantitative analysis. To address this
gap, we describe a postprocessing approach to create
ventilation difference maps to better visualize and quantify
regional ventilation changes before and after MCh challenge.
We show that difference maps reveal subtle changes in airway
caliber, and highlight both focal and diffuse regional
alterations in ventilation. Ventilation changes include both
hypoventilation and compensatory areas of hyperventilation.
The difference maps can be quantified by a histogram plot of
the ventilation changes, in which the standard deviation
increases with MCh dose (R(2) = 0.89). This method of
analysis is shown to be more sensitive than simple
threshold-based detection of gross ventilation
defects.},
Language = {eng},
Doi = {10.1002/mrm.22311},
Key = {fds268834}
}
@article{fds268828,
Author = {Lin, M and Marshall, CT and Qi, Y and Johnston, SM and Badea, CT and Piantadosi, CA and Johnson, GA},
Title = {Quantitative blood flow measurements in the small animal
cardiopulmonary system using digital subtraction
angiography.},
Journal = {Med Phys},
Volume = {36},
Number = {11},
Pages = {5347-5358},
Year = {2009},
Month = {November},
ISSN = {0094-2405},
url = {http://www.ncbi.nlm.nih.gov/pubmed/19994543},
Keywords = {Angiography, Digital Subtraction • Animals •
Calibration • Heart • Lung • Male •
Phantoms, Imaging • Rats • Rats, Inbred F344
• Regional Blood Flow* • Thermodilution •
anatomy & histology • methods* •
physiology*},
Abstract = {PURPOSE: The use of preclinical rodent models of disease
continues to grow because these models help elucidate
pathogenic mechanisms and provide robust test beds for drug
development. Among the major anatomic and physiologic
indicators of disease progression and genetic or drug
modification of responses are measurements of blood vessel
caliber and flow. Moreover, cardiopulmonary blood flow is a
critical indicator of gas exchange. Current methods of
measuring cardiopulmonary blood flow suffer from some or all
of the following limitations--they produce relative values,
are limited to global measurements, do not provide
vasculature visualization, are not able to measure acute
changes, are invasive, or require euthanasia. METHODS: In
this study, high-spatial and high-temporal resolution x-ray
digital subtraction angiography (DSA) was used to obtain
vasculature visualization, quantitative blood flow in
absolute metrics (ml/min instead of arbitrary units or
velocity), and relative blood volume dynamics from discrete
regions of interest on a pixel-by-pixel basis (100 x 100
microm2). RESULTS: A series of calibrations linked the DSA
flow measurements to standard physiological measurement
using thermodilution and Fick's method for cardiac output
(CO), which in eight anesthetized Fischer-344 rats was found
to be 37.0 +/- 5.1 ml/min. Phantom experiments were
conducted to calibrate the radiographic density to vessel
thickness, allowing a link of DSA cardiac output
measurements to cardiopulmonary blood flow measurements in
discrete regions of interest. The scaling factor linking
relative DSA cardiac output measurements to the Fick's
absolute measurements was found to be 18.90 x CODSA =
COFick. CONCLUSIONS: This calibrated DSA approach allows
repeated simultaneous visualization of vasculature and
measurement of blood flow dynamics on a regional level in
the living rat.},
Language = {eng},
Doi = {10.1118/1.3231823},
Key = {fds268828}
}
@article{fds268725,
Author = {Argyridis, I and Li, W and Johnson, GA and Liu, C},
Title = {Quantitative magnetic susceptibility of the developing mouse
brain reveals microstructural changes in the white
matter.},
Journal = {Neuroimage},
Volume = {88},
Pages = {134-142},
Year = {2014},
Month = {March},
url = {http://www.ncbi.nlm.nih.gov/pubmed/24269576},
Abstract = {Cerebral development involves a complex cascade of events
which are difficult to visualize and quantify in vivo. In
this study we combine information from Diffusion Tensor
Imaging (DTI) and Quantitative Susceptibility Mapping (QSM)
to analyze developing mouse brains at five stages up to
56days postnatal. Susceptibility maps were calculated using
frequency shifts in gradient echo MR images acquired at
9.4T. The mean apparent magnetic susceptibility and magnetic
susceptibility anisotropy of major white matter tracts were
evaluated as a function of age. During the first two weeks,
susceptibility of white matter appeared paramagnetic
relative to surrounding gray matter; it then gradually
became more diamagnetic. While diffusion anisotropy was
already apparent and high at postnatal day 2, susceptibility
anisotropy only became significant during the third week.
This mismatch indicated different microstructural
underpinnings for diffusion anisotropy and susceptibility
anisotropy. Histological exams were also performed to
evaluate myelin and iron content. It is confirmed that the
main source of susceptibility contrast in WM is the myelin
content. The ability to quantify the magnetic properties of
white matter will provide valuable information on the
architecture of the brain during development and potentially
a more specific indicator for myelin degenerative
diseases.},
Doi = {10.1016/j.neuroimage.2013.11.026},
Key = {fds268725}
}
@article{fds268718,
Author = {Johnson, GA and Calabrese, E and Little, PB and Hedlund, L and Qi, Y and Badea, A},
Title = {Quantitative mapping of trimethyltin injury in the rat brain
using magnetic resonance histology.},
Journal = {Neurotoxicology},
Volume = {42},
Pages = {12-23},
Year = {2014},
Month = {May},
ISSN = {0161-813X},
url = {http://hdl.handle.net/10161/10329 Duke open
access},
Abstract = {The growing exposure to chemicals in our environment and the
increasing concern over their impact on health have elevated
the need for new methods for surveying the detrimental
effects of these compounds. Today's gold standard for
assessing the effects of toxicants on the brain is based on
hematoxylin and eosin (H&E)-stained histology, sometimes
accompanied by special stains or immunohistochemistry for
neural processes and myelin. This approach is time-consuming
and is usually limited to a fraction of the total brain
volume. We demonstrate that magnetic resonance histology
(MRH) can be used for quantitatively assessing the effects
of central nervous system toxicants in rat models. We show
that subtle and sparse changes to brain structure can be
detected using magnetic resonance histology, and correspond
to some of the locations in which lesions are found by
traditional pathological examination. We report for the
first time diffusion tensor image-based detection of changes
in white matter regions, including fimbria and corpus
callosum, in the brains of rats exposed to 8 mg/kg and 12
mg/kg trimethyltin. Besides detecting brain-wide changes,
magnetic resonance histology provides a quantitative
assessment of dose-dependent effects. These effects can be
found in different magnetic resonance contrast mechanisms,
providing multivariate biomarkers for the same spatial
location. In this study, deformation-based morphometry
detected areas where previous studies have detected cell
loss, while voxel-wise analyses of diffusion tensor
parameters revealed microstructural changes due to such
things as cellular swelling, apoptosis, and inflammation.
Magnetic resonance histology brings a valuable addition to
pathology with the ability to generate brain-wide
quantitative parametric maps for markers of toxic insults in
the rodent brain.},
Doi = {10.1016/j.neuro.2014.02.009},
Key = {fds268718}
}
@article{fds268796,
Author = {Badea, A and Gewalt, S and Avants, BB and Cook, JJ and Johnson,
GA},
Title = {Quantitative mouse brain phenotyping based on single and
multispectral MR protocols.},
Journal = {Neuroimage},
Volume = {63},
Number = {3},
Pages = {1633-1645},
Year = {2012},
Month = {November},
url = {http://www.ncbi.nlm.nih.gov/pubmed/22836174},
Abstract = {Sophisticated image analysis methods have been developed for
the human brain, but such tools still need to be adapted and
optimized for quantitative small animal imaging. We propose
a framework for quantitative anatomical phenotyping in mouse
models of neurological and psychiatric conditions. The
framework encompasses an atlas space, image acquisition
protocols, and software tools to register images into this
space. We show that a suite of segmentation tools (Avants,
Epstein et al., 2008) designed for human neuroimaging can be
incorporated into a pipeline for segmenting mouse brain
images acquired with multispectral magnetic resonance
imaging (MR) protocols. We present a flexible approach for
segmenting such hyperimages, optimizing registration, and
identifying optimal combinations of image channels for
particular structures. Brain imaging with T1, T2* and T2
contrasts yielded accuracy in the range of 83% for
hippocampus and caudate putamen (Hc and CPu), but only 54%
in white matter tracts, and 44% for the ventricles. The
addition of diffusion tensor parameter images improved
accuracy for large gray matter structures (by >5%), white
matter (10%), and ventricles (15%). The use of Markov random
field segmentation further improved overall accuracy in the
C57BL/6 strain by 6%; so Dice coefficients for Hc and CPu
reached 93%, for white matter 79%, for ventricles 68%, and
for substantia nigra 80%. We demonstrate the segmentation
pipeline for the widely used C57BL/6 strain, and two test
strains (BXD29, APP/TTA). This approach appears promising
for characterizing temporal changes in mouse models of human
neurological and psychiatric conditions, and may provide
anatomical constraints for other preclinical imaging, e.g.
fMRI and molecular imaging. This is the first demonstration
that multiple MR imaging modalities combined with
multivariate segmentation methods lead to significant
improvements in anatomical segmentation in the mouse
brain.},
Doi = {10.1016/j.neuroimage.2012.07.021},
Key = {fds268796}
}
@article{fds268792,
Author = {Johnson, GA and Badea, A and Jiang, Y},
Title = {Quantitative neuromorphometry using magnetic resonance
histology.},
Journal = {Toxicol Pathol},
Volume = {39},
Number = {1},
Pages = {85-91},
Year = {2011},
Month = {January},
url = {http://www.ncbi.nlm.nih.gov/pubmed/21119052},
Abstract = {Magnetic resonance imaging (MRI), now common in the clinical
domain, has been adapted for use by the neuropathologist by
increasing the spatial resolution over 100,000 times what is
common in human clinical imaging. This increase in spatial
resolution has been accomplished through a variety of
technical advances-higher magnetic fields, more sensitive
receivers, and clever encoding methods. Magnetic resonance
histology (MRH), that is, the application of MRI to study
tissue specimens, now makes three-dimensional imaging of the
fixed brain in the cranium routine. Active staining
(perfusion fixation with a paramagnetic contrast agent) has
allowed us to reduce the scan time by more than 8 times over
earlier methods. The result is a three-dimensional isotropic
image array that can be viewed along any direction without
loss of spatial resolution. Homologous slices can be chosen
interactively. Since the tissue is still fully hydrated in
the cranium, tissue shrinkage and distortion are virtually
eliminated. Volume measurements of neural structures can be
made with a high degree of precision and accuracy. MRH will
not replace more traditional methods, but it promises
enormous value in choosing particular areas and times for
more traditional sectioning and assessment.},
Doi = {10.1177/0192623310389622},
Key = {fds268792}
}
@article{fds132838,
Author = {H Benveniste and GP Cofer and CA Piantadosi and JN Davis and GA
Johnson},
Title = {Quantitative proton magnetic resonance imaging in focal
cerebral ischemia in rat brain.},
Journal = {Stroke; a journal of cerebral circulation, UNITED
STATES},
Volume = {22},
Number = {2},
Pages = {259-68},
Year = {1991},
Month = {February},
ISSN = {0039-2499},
Keywords = {Animals • Brain • Brain Ischemia • Cerebral
Infarction • Diagnosis, Computer-Assisted •
Magnetic Resonance Imaging* • Necrosis • Rats
• Rats, Inbred Strains • Silver • diagnosis
• diagnosis* • diagnostic use • pathology
• pathology*},
Abstract = {Proton magnetic resonance (MR) imaging has been recommended
as a diagnostic tool for the detection of focal cerebral
ischemia. We compared microscopic MR images of rat brains
after focal cerebral ischemia with evidence of histological
damage found on corresponding silver-impregnated or cresyl
violet-stained brain sections. Ten male Wistar rats were
subjected to permanent unilateral occlusions of the right
middle cerebral and common carotid arteries under halothane
anesthesia. Twenty-four hours later the area of injury on MR
images amounted to 26% of the total slice area, whereas only
9% of the total slice area was necrotic on histological
sections from the same animals. The infarcted areas on
tissue sections were surrounded by regions of selective
neuronal injury in the cerebral cortex and occasionally in
the hippocampus. The area of injury on MR images was larger
than the combined areas of infarction and selective neuronal
injury on histological sections. Areas of increased T2
values on MR images extended medially into noninfarcted
striatum and laterally and dorsally into noninfarcted
cortex. The lateral and dorsal areas on MR images frequently
coincided with cortical areas in which considerable
selective neuronal injury was present in the upper cortical
layers. We hypothesize that the abnormal areas on MR images
above histologically normal brain tissue represent the
ischemic penumbra. If true, this is the first demonstration
of the ischemic penumbra by MR imaging and may reflect our
use of Wistar rats, a new image analysis technique, and
ultra-high resolution MR imaging.},
Key = {fds132838}
}
@booklet{Benveniste91,
Author = {Benveniste, H and Cofer, GP and Piantadosi, CA and Davis, JN and Johnson, GA},
Title = {Quantitative proton magnetic resonance imaging in focal
cerebral ischemia in rat brain.},
Journal = {Stroke},
Volume = {22},
Number = {2},
Pages = {259-268},
Year = {1991},
Month = {February},
ISSN = {0039-2499},
url = {http://www.ncbi.nlm.nih.gov/pubmed/2003291},
Abstract = {Proton magnetic resonance (MR) imaging has been recommended
as a diagnostic tool for the detection of focal cerebral
ischemia. We compared microscopic MR images of rat brains
after focal cerebral ischemia with evidence of histological
damage found on corresponding silver-impregnated or cresyl
violet-stained brain sections. Ten male Wistar rats were
subjected to permanent unilateral occlusions of the right
middle cerebral and common carotid arteries under halothane
anesthesia. Twenty-four hours later the area of injury on MR
images amounted to 26% of the total slice area, whereas only
9% of the total slice area was necrotic on histological
sections from the same animals. The infarcted areas on
tissue sections were surrounded by regions of selective
neuronal injury in the cerebral cortex and occasionally in
the hippocampus. The area of injury on MR images was larger
than the combined areas of infarction and selective neuronal
injury on histological sections. Areas of increased T2
values on MR images extended medially into noninfarcted
striatum and laterally and dorsally into noninfarcted
cortex. The lateral and dorsal areas on MR images frequently
coincided with cortical areas in which considerable
selective neuronal injury was present in the upper cortical
layers. We hypothesize that the abnormal areas on MR images
above histologically normal brain tissue represent the
ischemic penumbra. If true, this is the first demonstration
of the ischemic penumbra by MR imaging and may reflect our
use of Wistar rats, a new image analysis technique, and
ultra-high resolution MR imaging.},
Doi = {10.1161/01.str.22.2.259},
Key = {Benveniste91}
}
@article{fds268726,
Author = {Xie, L and Sparks, MA and Li, W and Qi, Y and Liu, C and Coffman, TM and Johnson, GA},
Title = {Quantitative susceptibility mapping of kidney inflammation
and fibrosis in type 1 angiotensin receptor-deficient
mice.},
Journal = {NMR Biomed},
Volume = {26},
Number = {12},
Pages = {1853-1863},
Year = {2013},
Month = {December},
url = {http://www.ncbi.nlm.nih.gov/pubmed/24154952},
Abstract = {Disruption of the regulatory role of the kidneys leads to
diverse renal pathologies; one major hallmark is
inflammation and fibrosis. Conventional magnitude MRI has
been used to study renal pathologies; however, the
quantification or even detection of focal lesions caused by
inflammation and fibrosis is challenging. We propose that
quantitative susceptibility mapping (QSM) may be
particularly sensitive for the identification of
inflammation and fibrosis. In this study, we applied QSM in
a mouse model deficient for angiotensin receptor type 1
(AT1). This model is known for graded pathologies, including
focal interstitial fibrosis, cortical inflammation,
glomerulocysts and inner medullary hypoplasia. We acquired
high-resolution MRI on kidneys from AT1-deficient mice that
were perfusion fixed with contrast agent. Two MR sequences
were used (three-dimensional spin echo and gradient echo) to
produce three image contrasts: T1, T2* (magnitude) and QSM.
T1 and T2* (magnitude) images were acquired to segment major
renal structures and to provide landmarks for the focal
lesions of inflammation and fibrosis in the
three-dimensional space. The volumes of major renal
structures were measured to determine the relationship of
the volumes to the degree of renal abnormalities and
magnetic susceptibility values. Focal lesions were segmented
from QSM images and were found to be closely associated with
the major vessels. Susceptibilities were relatively more
paramagnetic in wild-type mice: 1.46 ± 0.36 in the cortex,
2.14 ± 0.94 in the outer medulla and 2.10 ± 2.80 in the
inner medulla (10(-2) ppm). Susceptibilities were more
diamagnetic in knockout mice: -7.68 ± 4.22 in the cortex,
-11.46 ± 2.13 in the outer medulla and -7.57 ± 5.58 in the
inner medulla (10(-2) ppm). This result was consistent with
the increase in diamagnetic content, e.g. proteins and
lipids, associated with inflammation and fibrosis. Focal
lesions were validated with conventional histology. QSM was
very sensitive in detecting pathology caused by small focal
inflammation and fibrosis. QSM offers a new MR contrast
mechanism to study this common disease marker in the
kidney.},
Doi = {10.1002/nbm.3039},
Key = {fds268726}
}
@article{fds132732,
Author = {G.A. Johnson},
Title = {R.D. Black, H. Middleton, G.D. Cates, G.P. Cofer, B.
Driehuys, W. Happer, L. W. Hedlund, G.A. Johnson, M.D.
Shattuck, J. Swartz, In vivo He-3 MR Images of guinea pig
lungs. Radiology 199, 867-870 (1996).},
Year = {1996},
Key = {fds132732}
}
@article{fds132718,
Author = {G.A. Johnson},
Title = {R.T. Engelhardt, G.A. Johnson, T1r relaxation and its
application to MR histology. Magnetic Resonance in
Medicine 35, 781-786 (1996).},
Year = {1996},
Key = {fds132718}
}
@booklet{Frush01,
Author = {Frush, DP and Yoshizumi, TT and Paulson, EK and Johnson,
GA},
Title = {Radiation dose from helical CT in children: Comparison of
multi-slice and single-slice protocols},
Journal = {RADIOLOGY},
Volume = {221},
Pages = {246-246},
Publisher = {RADIOLOGICAL SOC NORTH AMERICA},
Year = {2001},
Month = {November},
ISSN = {0033-8419},
url = {http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000172126600589&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=47d3190e77e5a3a53558812f597b0b92},
Key = {Frush01}
}
@article{8979178,
Author = {Kelly, W.M. and Low, N.M. and Zillmer, A. and Johnson, G.A. and Normand, E.},
Title = {Radiation environments and exposure considerations for the
Multi-Mission Radioisotope Thermoelectric
Generator},
Journal = {AIP Conf. Proc. (USA)},
Number = {813},
Pages = {906 - 19},
Year = {2006},
Keywords = {electromagnetic shielding;magnetic fields;radiation;radioisotope
thermoelectric generators;},
Abstract = {The Multi-Mission Radioisotope Thermoelectric Generator
(MMRTG) is the next generation (RTG) being developed by DOE
to provide reliable, long-life electric power for NASA's
planetary exploration programs. The MMRTG is being developed
by Pratt & Whitney Rocketdyne and Teledyne Energy
Systems Incorporated (TESI) for use on currently planned and
projected flyby, orbital and planet landing missions. This
is a significant departure from the design philosophy of the
past which was to match specific mission requirements to RTG
design capabilities. Undefined mission requirements provide
a challenge to system designers by forcing them to put a
design envelope around "all possible missions". These
multi-mission requirements include internal and external
radiation sources. Internal sources include the particles
ejected by decaying Pu-238 and its daughters plus particles
resulting from the interaction of these particles with other
MMRTG materials. External sources include the full spectrum
of charged particle radiation surrounding planets with
magnetic fields and the surfaces of extraterrestrial objects
not shielded by magnetic fields. The paper presents the
results of investigations into the environments outlined
above and the impact of radiation exposure on potential
materials to be used on MMRTG and ground support personnel.
Mission requirements were also reviewed to evaluate total
integrated dose and to project potential shielding
requirements for materials. Much of the information on
mission shielding requirements was provided by NASA's Jet
Propulsion Laboratory. The primary result is an ionizing
radiation design curve which indicates the limits to which a
particular mission can take the MMRTG in terms of ionizing
radiation exposure. Estimates of personnel radiation
exposure during ground handling are also
provided},
Key = {8979178}
}
@article{fds174132,
Author = {CA Baker and GA Johnson},
Title = {Radioenzymatic assay of dihydroxyphenylglycol (DOPEG) and
dihydroxyphenylethanol (DOPET) in plasma and cerebrospinal
fluid.},
Journal = {Life sciences},
Volume = {29},
Number = {2},
Pages = {165-72},
Year = {1981},
Month = {July},
ISSN = {0024-3205},
Keywords = {Adult • Aged • Animals • Cats • Dogs
• Ethanol • Female • Glycols • Humans
• Infant • Male • Methoxyhydroxyphenylglycol
• Middle Aged • Phenylethyl Alcohol •
Radioisotope Dilution Technique • Rats •
S-Adenosylmethionine • Tritium • analogs &
derivatives • analogs & derivatives* • blood
• blood* • cerebrospinal fluid},
Language = {eng},
Key = {fds174132}
}
@booklet{Baker81,
Author = {C. A. Baker and G. A. Johnson},
Title = {Radioenzymatic assay of dihydroxyphenylglycol (dopeg) and
dihydroxyphenylethanol (dopet) in plasma and
cerebrospinal-fluid},
Journal = {Life Sciences},
Volume = {29},
Number = {2},
Pages = {165 -- 172},
Year = {1981},
Key = {Baker81}
}
@article{fds174098,
Author = {GA Johnson and JM Gren and R Kupiecki},
Title = {Radioenzymatic assay of DOPA (3,4-dihydroxyphenylalanine).},
Journal = {Clinical chemistry},
Volume = {24},
Number = {11},
Pages = {1927-30},
Year = {1978},
Month = {November},
ISSN = {0009-9147},
Keywords = {Adult • Animals • Catechol O-Methyltransferase
• Dihydroxyphenylalanine • Dopa Decarboxylase
• Female • Humans • Male • analysis*
• blood},
Abstract = {We modified the single-isotope radioenzymatic assay for
catecholamines [Life Sci. 21, 625 (1977)] to assay 3,
4-dihydroxyphenylalanine (DOPA). DOPA decarboxylase is used
to convert DOPA to dopamine, which concurrently is converted
to [3H]-3-O-methyldopamine in the presence of
catechol-O-methyltransferase and [methyl-3H]-S-adenosylmethionine
and assayed radioenzymatically. For assay of plasma DOPA, 50
microliter of untreated plasma is added directly into the
incubation mixture. A duplicate mixture containing an
internal standard requires a second 50-microliter aliquot of
plasma. Because the assay measures both DOPA and endogenous
dopamine, two additonal aliquots of plasma must be assayed
for dopamine in the absence of the decarboxylase by the
differential assay; DOPA is estimated by difference. The
assay is sensitive to 25 pg (500 ng/liter of plasma).
Analysis of DOPA (DOPA plus dopamine) and the concurrent
differential assay of catecholamines in at least 10 samples
can be done in a single working day. Plasma DOPA
concentrations for 42 normotensive adults were 1430 +/- 19
ng/liter (mean +/- SEM). In contrast, do-pamine
concentrations for these same subjects averaged 23 +/- 20
ng/liter. Values for the 24 women subjects (15 10 +/- 62
ng/liter) significantly (P = 0.04) exceeded those for the
men (1320 +/- 75 ng/liter).},
Language = {eng},
Key = {fds174098}
}
@booklet{Johnson80c,
Author = {G. A. Johnson and C. A. Baker and R. T. Smith},
Title = {Radioenzymatic assay of sulfate conjugates of catecholamines
and dopa in plasma},
Journal = {Life Sciences},
Volume = {26},
Number = {19},
Pages = {1591 -- 1598},
Year = {1980},
Key = {Johnson80c}
}
@article{fds174262,
Author = {GA Johnson and CA Baker and RT Smith},
Title = {Radioenzymatic assay of sulfate conjugates of catecholamines
and DOPA in plasma.},
Journal = {Life sciences},
Volume = {26},
Number = {19},
Pages = {1591-8},
Year = {1980},
Month = {May},
ISSN = {0024-3205},
Keywords = {Adolescent • Adult • Arylsulfatases •
Catecholamines • Child • Child, Preschool •
Dihydroxyphenylalanine • Dopamine • Epinephrine
• Female • Humans • Infant • Male •
Norepinephrine • Posture • Radioimmunoassay •
Sulfuric Acid Esters • blood •
blood*},
Language = {eng},
Key = {fds174262}
}
@booklet{Fram87,
Author = {Fram, EK and Herfkens, RJ and Johnson, GA and Glover, GH and Karis, JP and Shimakawa, A and Perkins, TG and Pelc, NJ},
Title = {Rapid calculation of T1 using variable flip angle gradient
refocused imaging.},
Journal = {Magn Reson Imaging},
Volume = {5},
Number = {3},
Pages = {201-208},
Year = {1987},
ISSN = {0730-725X},
url = {http://www.ncbi.nlm.nih.gov/pubmed/3626789},
Abstract = {We present a method for rapid measurement of T1 relaxation
times using gradient refocused images at limited flip angles
and short repetition times. This "variable nutation"
techniques was investigated using a T1 phantom. There was a
high correlation between measurements obtained with the
variable nutation and partial saturation techniques. The
ability of this method to create calculated T1 images is
also demonstrated. We conclude that the variable nutation
method may allow measurement of T1 relaxation times with a
significant reduction in acquisition time compared to
partial saturation techniques.},
Doi = {10.1016/0730-725x(87)90021-x},
Key = {Fram87}
}
@article{fds132800,
Author = {EK Fram and RJ Herfkens and GA Johnson and GH Glover and JP Karis and A
Shimakawa, TG Perkins and NJ Pelc},
Title = {Rapid calculation of T1 using variable flip angle gradient
refocused imaging.},
Journal = {Magnetic resonance imaging, UNITED STATES},
Volume = {5},
Number = {3},
Pages = {201-8},
Year = {1987},
ISSN = {0730-725X},
Keywords = {Humans • Magnetic Resonance Spectroscopy •
diagnostic use* • methods},
Abstract = {We present a method for rapid measurement of T1 relaxation
times using gradient refocused images at limited flip angles
and short repetition times. This "variable nutation"
techniques was investigated using a T1 phantom. There was a
high correlation between measurements obtained with the
variable nutation and partial saturation techniques. The
ability of this method to create calculated T1 images is
also demonstrated. We conclude that the variable nutation
method may allow measurement of T1 relaxation times with a
significant reduction in acquisition time compared to
partial saturation techniques.},
Key = {fds132800}
}
@article{fds268845,
Author = {Howles, GP and Nouls, JC and Qi, Y and Johnson, GA},
Title = {Rapid production of specialized animal handling devices
using computer-aided design and solid freeform
fabrication.},
Journal = {J Magn Reson Imaging},
Volume = {30},
Number = {2},
Pages = {466-471},
Year = {2009},
Month = {August},
ISSN = {1053-1807},
url = {http://www.ncbi.nlm.nih.gov/pubmed/19629999},
Abstract = {PURPOSE: To develop a process for rapidly and inexpensively
producing customized animal handling devices for small
animal imaging. MATERIALS AND METHODS: To meet the specific
needs of a particular imaging experiment, measurements are
taken from imaging data and the animal handling devices are
designed using 3D computer-aided design (CAD) software.
Parts are produced in a few days using solid freeform
fabrication (SFF, a.k.a. rapid prototyping). RESULTS: This
process is illustrated with the production of an animal
handling system for stereotaxically prescribed therapeutic
ultrasound and MRI of the mouse brain. The device provides
integrated head-fixation, anesthesia delivery, and
physiological monitoring in a modular system. Design and
production took approximately 1 week and the cost was a
small fraction of a traditional machine shop. CONCLUSION:
Commercial animal handling products typically have limited
functionality and are not integrated with other laboratory
infrastructure. However, using CAD and SFF, sophisticated
animal handling devices can be produced to meet the specific
experimental needs. This process is typically faster and
less expensive than using a traditional machine shop, and
the products are more robust than typical homemade devices.
Using high-quality purpose-built devices permits experiments
to be executed with greater consistency and higher
throughput.},
Doi = {10.1002/jmri.21821},
Key = {fds268845}
}
@booklet{Mermelstein01,
Author = {M. D. Mermelstein and R. Posey and G. A. Johnson and S. T.
Vohra},
Title = {Rayleigh scattering optical frequency correlation in a
single-mode optical fiber},
Journal = {Optics Letters},
Volume = {26},
Number = {2},
Pages = {58 -- 60},
Year = {2001},
Month = {January},
Key = {Mermelstein01}
}
@article{fds174083,
Author = {MD Mermelstein and R Posey Jr and GA Johnson and ST
Vohra},
Title = {Rayleigh scattering optical frequency correlation in a
single-mode optical fiber.},
Journal = {Optics letters},
Volume = {26},
Number = {2},
Pages = {58-60},
Year = {2001},
Month = {January},
ISSN = {0146-9592},
Abstract = {The bichromatic optical frequency correlation function for
Rayleigh backscattering from a pulse of laser light
propagating along a single-mode optical fiber has been
calculated and measured. It is shown that the optical
correlation frequency, Dnu(c) , is equal to the reciprocal
of pulse width T(w) . These results are important for the
development of wavelength diversity techniques for the
reduction of coherent Rayleigh noise in distributed Rayleigh
backscattering single-mode optical fiber
sensors.},
Language = {eng},
Key = {fds174083}
}
@article{fds268827,
Author = {Pandit, P and Qi, Y and King, KF and Johnson, GA},
Title = {Reduction of artifacts in T2 -weighted PROPELLER in
high-field preclinical imaging.},
Journal = {Magn Reson Med},
Volume = {65},
Number = {2},
Pages = {538-543},
Year = {2011},
Month = {February},
ISSN = {1522-2594},
url = {http://www.ncbi.nlm.nih.gov/pubmed/20928875},
Keywords = {Animals • Artifacts* • Image Enhancement •
Lung Neoplasms • Magnetic Resonance Imaging • Mice
• Neoplasm Transplantation • Phantoms, Imaging
• diagnosis • methods*},
Abstract = {A simple technique is implemented for correction of
artifacts arising from nonuniform T(2) -weighting of k-space
data in fast spin echo-based PROPELLER (periodically rotated
overlapping parallel lines with enhanced reconstruction). An
additional blade with no phase-encoding gradients is
acquired to generate the scaling factor used for the
correction. Results from simulations and phantom
experiments, as well as in vivo experiments in
free-breathing mice, demonstrate the advantages of the
proposed method. This technique is developed specifically
for high-field imaging applications where T(2) decay is
rapid.},
Language = {eng},
Doi = {10.1002/mrm.22624},
Key = {fds268827}
}
@booklet{Zhou93,
Author = {Zhou, X and Liang, ZP and Cofer, GP and Beaulieu, CF and Suddarth, SA and Johnson, GA},
Title = {Reduction of ringing and blurring artifacts in fast
spin-echo imaging.},
Journal = {J Magn Reson Imaging},
Volume = {3},
Number = {5},
Pages = {803-807},
Year = {1993},
ISSN = {1053-1807},
url = {http://www.ncbi.nlm.nih.gov/pubmed/8400569},
Abstract = {A simple method was devised to reduce ringing and blurring
artifacts caused by discontinuous T2 weighting of k-space
data in fast spin-echo magnetic resonance (MR) imaging. The
method demodulates the weighting function along the
phase-encoding direction by using multiple T2 values derived
from a set of non-phase-encoded echoes obtained from an
extra excitation. The performance of this method was
evaluated by computer simulations and experiments, which
confirmed its capability of effectively reducing or, in some
cases, even completely removing the ringing and blurring
artifacts. The results also show that the proposed method
produces better results than other artifact reduction
methods. The method is particularly useful at high magnetic
field strengths (7.1-9.4 T) and with strong gradients (> 20
G/cm) used in MR microscopy, in which the apparent T2 values
are short for most tissues. The authors expect that the
proposed method will find useful applications in various
fast spin-echo pulse sequences.},
Doi = {10.1002/jmri.1880030518},
Key = {Zhou93}
}
@article{fds132871,
Author = {X Zhou and ZP Liang and GP Cofer and CF Beaulieu and SA Suddarth and GA
Johnson},
Title = {Reduction of ringing and blurring artifacts in fast
spin-echo imaging.},
Journal = {Journal of magnetic resonance imaging : JMRI, UNITED
STATES},
Volume = {3},
Number = {5},
Pages = {803-7},
ISSN = {1053-1807},
Keywords = {Artifacts* • Image Enhancement • Magnetic
Resonance Imaging • Models, Structural • methods
• methods*},
Abstract = {A simple method was devised to reduce ringing and blurring
artifacts caused by discontinuous T2 weighting of k-space
data in fast spin-echo magnetic resonance (MR) imaging. The
method demodulates the weighting function along the
phase-encoding direction by using multiple T2 values derived
from a set of non-phase-encoded echoes obtained from an
extra excitation. The performance of this method was
evaluated by computer simulations and experiments, which
confirmed its capability of effectively reducing or, in some
cases, even completely removing the ringing and blurring
artifacts. The results also show that the proposed method
produces better results than other artifact reduction
methods. The method is particularly useful at high magnetic
field strengths (7.1-9.4 T) and with strong gradients (> 20
G/cm) used in MR microscopy, in which the apparent T2 values
are short for most tissues. The authors expect that the
proposed method will find useful applications in various
fast spin-echo pulse sequences.},
Key = {fds132871}
}
@booklet{Sherrier87,
Author = {Sherrier, RH and Johnson, GA},
Title = {Regionally adaptive histogram equalization of the
chest.},
Journal = {IEEE Trans Med Imaging},
Volume = {6},
Number = {1},
Pages = {1-7},
Year = {1987},
ISSN = {0278-0062},
url = {http://www.ncbi.nlm.nih.gov/pubmed/18230420},
Abstract = {Advances in the area of digital chest radiography have
resulted in the acquisition of high-quality images of the
human chest. With these advances, there arises a genuine
need for image processing algorithms specific to the chest,
in order to fully exploit this digital technology. We have
implemented the well-known technique of histogram
equalization, noting the problems encountered when it is
adapted to chest images. These problems have been
successfully solved with our regionally adaptive histogram
equalization method. With this technique histograms are
calculated locally and then modified according to both the
mean pixel value of that region as well as certain
characteristics of the cumulative distribution function.
This process, which has allowed certain regions of the chest
radiograph to be enhanced differentially, may also have
broader implications for other image processing
tasks.},
Doi = {10.1109/TMI.1987.4307791},
Key = {Sherrier87}
}
@article{fds292757,
Author = {Sherrier, RH and Johnson, GA},
Title = {REGIONALLY ADAPTIVE HISTOGRAM EQUALIZATION OF THE
CHEST.},
Journal = {IEEE Transactions on Medical Imaging},
Volume = {MI-6},
Number = {1},
Pages = {1-7},
Year = {1987},
Abstract = {The authors have implemented the well-known technique of
histogram equalization, noting the problems encountered when
it is adapted to chest images. These problems have been
successfully solved with a regionally adaptive histogram
equalization method. With this technique, histograms are
calculated locally and then modified according to both the
mean pixel value of that region as well as certain
characteristics of the cumulative distribution function.
This process, which has allowed certain regions of the chest
radiograph to be enhanced differentially, may also have
broader implications for other image processing
tasks.},
Key = {fds292757}
}
@article{fds157104,
Author = {RH Sherrier and GA Johnson},
Title = {Regionally adaptive histogram equalization of the
chest.},
Journal = {IEEE transactions on medical imaging, United
States},
Volume = {6},
Number = {1},
Pages = {1-7},
Year = {1987},
ISSN = {0278-0062},
Abstract = {Advances in the area of digital chest radiography have
resulted in the acquisition of high-quality images of the
human chest. With these advances, there arises a genuine
need for image processing algorithms specific to the chest,
in order to fully exploit this digital technology. We have
implemented the well-known technique of histogram
equalization, noting the problems encountered when it is
adapted to chest images. These problems have been
successfully solved with our regionally adaptive histogram
equalization method. With this technique histograms are
calculated locally and then modified according to both the
mean pixel value of that region as well as certain
characteristics of the cumulative distribution function.
This process, which has allowed certain regions of the chest
radiograph to be enhanced differentially, may also have
broader implications for other image processing
tasks.},
Key = {fds157104}
}
@article{fds268912,
Author = {Johnson, GA and Cofer, GP and Hedlund, LW and Maronpot, RR and Suddarth,
SA},
Title = {Registered (1)H and (3)He magnetic resonance microscopy of
the lung.},
Journal = {Magn Reson Med},
Volume = {45},
Number = {3},
Pages = {365-370},
Year = {2001},
Month = {March},
ISSN = {0740-3194},
url = {http://www.ncbi.nlm.nih.gov/pubmed/11241691},
Keywords = {Animals • Anisotropy • Helium* • Hydrogen*
• Image Enhancement* • Image Processing,
Computer-Assisted • Imaging, Three-Dimensional •
Isotopes • Lung • Magnetic Resonance Imaging
• Male • Microscopy • Rats • Rats,
Inbred F344 • Sensitivity and Specificity •
methods* • pathology*},
Abstract = {Using in vivo magnetic resonance microscopy, registered (1)H
and hyperpolarized (3)He images of the rat lung were
obtained with a resolution of 0.098 x 0.098 x 0.469 mm (4.5
x 10(-3) mm(3)). The requisite stability and SNR was
achieved through an integration of scan-synchronous
ventilation, dual-frequency RF coils, anisotropic projection
encoding, and variable RF excitation. The total acquisition
time was 21 min for the (3)He images and 64 min for the (1)H
image. Airways down to the 6th and 7th orders are clearly
visible. Magn Reson Med 45:365-370, 2001.},
Doi = {10.1002/1522-2594(200103)45:3<365::aid-mrm1047>3.0.co;2-0},
Key = {fds268912}
}
@article{fds268794,
Author = {Clark, D and Badea, A and Liu, Y and Johnson, GA and Badea,
CT},
Title = {Registration-based segmentation of murine 4D cardiac
micro-CT data using symmetric normalization.},
Journal = {Phys Med Biol},
Volume = {57},
Number = {19},
Pages = {6125-6145},
Year = {2012},
Month = {October},
url = {http://www.ncbi.nlm.nih.gov/pubmed/22971564},
Abstract = {Micro-CT can play an important role in preclinical studies
of cardiovascular disease because of its high spatial and
temporal resolution. Quantitative analysis of 4D cardiac
images requires segmentation of the cardiac chambers at each
time point, an extremely time consuming process if done
manually. To improve throughput this study proposes a
pipeline for registration-based segmentation and functional
analysis of 4D cardiac micro-CT data in the mouse. Following
optimization and validation using simulations, the pipeline
was applied to in vivo cardiac micro-CT data corresponding
to ten cardiac phases acquired in C57BL/6 mice (n = 5).
After edge-preserving smoothing with a novel adaptation of
4D bilateral filtration, one phase within each cardiac
sequence was manually segmented. Deformable registration was
used to propagate these labels to all other cardiac phases
for segmentation. The volumes of each cardiac chamber were
calculated and used to derive stroke volume, ejection
fraction, cardiac output, and cardiac index. Dice
coefficients and volume accuracies were used to compare
manual segmentations of two additional phases with their
corresponding propagated labels. Both measures were, on
average, >0.90 for the left ventricle and >0.80 for the
myocardium, the right ventricle, and the right atrium,
consistent with trends in inter- and intra-segmenter
variability. Segmentation of the left atrium was less
reliable. On average, the functional metrics of interest
were underestimated by 6.76% or more due to systematic label
propagation errors around atrioventricular valves; however,
execution of the pipeline was 80% faster than performing
analogous manual segmentation of each phase.},
Doi = {10.1088/0031-9155/57/19/6125},
Key = {fds268794}
}
@article{fds174201,
Author = {H Ka and S Al-Ramadan and DW Erikson and GA Johnson and RC Burghardt and TE
Spencer, LA Jaeger and FW Bazer},
Title = {Regulation of expression of fibroblast growth factor 7 in
the pig uterus by progesterone and estradiol.},
Journal = {Biology of reproduction},
Volume = {77},
Number = {1},
Pages = {172-80},
Year = {2007},
Month = {July},
ISSN = {0006-3363},
url = {http://dx.doi.org/10.1095/biolreprod.106.056309},
Keywords = {Animals • Estradiol • Female • Fibroblast
Growth Factor 7 • Gene Expression Regulation •
Progesterone • RNA, Messenger • Receptors,
Estrogen • Receptors, Progesterone • Uterus •
anatomy & histology • antagonists & inhibitors •
drug effects* • metabolism • metabolism* •
pharmacology*},
Abstract = {Fibroblast growth factor 7 (FGF7) stimulates cell
proliferation, differentiation, migration and angiogenesis.
The consensus is that FGF7, expressed by mesenchymal cells,
binds FGF receptor 2IIIb (FGFR2) on epithelia, thereby
mediating epithelial-mesenchymal interactions. The pig
uterus is unique in that FGF7 is expressed by the luminal
epithelium (LE) and FGFR2 is expressed by the LE, glandular
epithelium (GE), and trophectoderm to effect proliferation
and differentiated cell functions during conceptus
development and implantation. FGF7 expression by the uterine
LE of pigs increases between Days 9 and 12 of the estrus
cycle and pregnancy, as circulating concentrations of
progesterone increase, progesterone receptors (PGR) in the
uterine epithelia decrease, and the conceptuses secrete
estradiol-17beta (E(2)), for pregnancy recognition.
Furthermore, E(2) increases the expression of FGF7 in pig
uterine explants. The present study investigates the
relationships between progesterone, E(2), and their
receptors and the expression of FGF7 in the pig uterus in
vivo. Pigs were ovariectomized on Day 4 of the estrus cycle
and injected i.m. daily from Day 4 to Day 12 with either
corn oil (CO), progesterone (P4), P4 and ZK317,316 (PZK),
E(2), P4 and E(2) (PE), or P4 and ZK and E(2) (PZKE). All
gilts (n = 5/treatment) were hysterectomized on Day 12. The
results suggest that: 1) P4 is permissive to FGF7 expression
by down-regulating PGR in LE; 2) P4 stimulates PGR-positive
uterine stromal cells to release an unidentified
progestamedin that induces FGF7 expression by LE; 3) E(2)
and P4 can induce FGF7 when PGR are rendered nonfunctional
by ZK; and 4) E(2) from conceptuses interacts via estrogen
receptor alpha, but not estrogen receptor beta in LE to
induce maximal expression of FGF7 in LE on Day 12 of
pregnancy in pigs.},
Language = {eng},
Doi = {10.1095/biolreprod.106.056309},
Key = {fds174201}
}
@booklet{Ka00,
Author = {H. H. Ka and L. A. Jaeger and G. A. Johnson and T. E.
Spencer and F. W. Bazer},
Title = {Regulation of keratinocyte growth factor expression and its
function in the porcine uterus.},
Journal = {Biology Of Reproduction},
Volume = {62},
Pages = {297 -- 297},
Year = {2000},
Key = {Ka00}
}
@booklet{Spencer03a,
Author = {T. E. Spencer and G. A. Johnson and F. W. Bazer and G. Y.
Wu},
Title = {Regulation of placental nitric oxide synthesis by estrogen
and progesterone in pigs.},
Journal = {Biology Of Reproduction},
Volume = {68},
Pages = {363 -- 364},
Year = {2003},
Key = {Spencer03a}
}
@booklet{Zaunbrecher03,
Author = {G. M. Zaunbrecher and T. E. Spencer and R. C. Burghardt and M. M. Joyce and F. W. Bazer and G. A. Johnson},
Title = {Regulaton of glycosylation dependent cell adhesion molecule
1 (GlyCAM-1) and l-selectin expression in the pregnant ovine
uterus and placenta.},
Journal = {Biology Of Reproduction},
Volume = {68},
Pages = {243 -- 243},
Year = {2003},
Key = {Zaunbrecher03}
}
@article{fds174287,
Author = {GA JOHNSON and RH McCLUER},
Title = {Relation of sialic acid to Rho (D) antigen.},
Journal = {Proceedings of the Society for Experimental Biology and
Medicine. Society for Experimental Biology and Medicine (New
York, N.Y.)},
Volume = {107},
Pages = {692-4},
Year = {1961},
Month = {July},
ISSN = {0037-9727},
Keywords = {ANTIEMETICS/therapy* • CARBOHYDRASES/pharmacology*
• NEURAMINIC ACIDS/pharmacology*},
Language = {eng},
Key = {fds174287}
}
@booklet{Baker84,
Author = {C. A. Baker and J. P. Hansen and M. V. Williams and F. G.
Robinson and J. E. Rogers and R. A. Zandt and M. G. Wendling and G. A. Johnson},
Title = {Relationship between the physiologic activity of minoxidil
and the plasma and tissue-levels of minoxidil and minoxidil
sulfate},
Journal = {Federation Proceedings},
Volume = {43},
Number = {3},
Pages = {556 -- 556},
Year = {1984},
Key = {Baker84}
}
@article{fds132850,
Author = {SE Dockery and SA Suddarth and GA Johnson},
Title = {Relaxation measurements at 300 MHz using MR
microscopy.},
Journal = {Magnetic resonance in medicine : official journal of the
Society of Magnetic Resonance in Medicine / Society of
Magnetic Resonance in Medicine, UNITED STATES},
Volume = {11},
Number = {2},
Pages = {182-92},
Year = {1989},
Month = {August},
ISSN = {0740-3194},
Keywords = {Animals • Kidney • Magnetic Resonance Imaging*
• Microscopy • Rats • Rats, Inbred F344
• anatomy & histology • methods},
Abstract = {Previous data on changes in tissue T1 with field have
suggested the convergence of tissue T1 values toward a
common value at high (greater than 4.0 T) fields. Measures
of T2 dependence have suggested reduction of T2 with field.
The purpose of this study was to observe the T1 and T2 at
85.5 and 300 MHz of microstructures in excised rat kidneys
by employing MR microscopy. This study represents the first
attempt of MR microscopy at 7.0 T with regard to the subject
of magnetic field dependence of T1 and T2. As expected, T1
did increase with increasing field strength but not as
dramatically as might be expected. Subtle differences in the
microstructures of the kidney and the binding of water in
those structures were discernible on the basis of T1
differences at 300 MHz. T2 values decreased, raising
speculation concerning the mechanism for this dependence.
The improved SNR permits smaller samples to be examined at
much higher resolutions (greater than 30 X 30 X 200
microns), further extending the potentials for MR
microscopy.},
Key = {fds132850}
}
@booklet{Dockery89,
Author = {Dockery, SE and Suddarth, SA and Johnson, GA},
Title = {Relaxation measurements at 300 MHz using MR
microscopy.},
Journal = {Magn Reson Med},
Volume = {11},
Number = {2},
Pages = {182-192},
Year = {1989},
Month = {August},
ISSN = {0740-3194},
url = {http://www.ncbi.nlm.nih.gov/pubmed/2779411},
Abstract = {Previous data on changes in tissue T1 with field have
suggested the convergence of tissue T1 values toward a
common value at high (greater than 4.0 T) fields. Measures
of T2 dependence have suggested reduction of T2 with field.
The purpose of this study was to observe the T1 and T2 at
85.5 and 300 MHz of microstructures in excised rat kidneys
by employing MR microscopy. This study represents the first
attempt of MR microscopy at 7.0 T with regard to the subject
of magnetic field dependence of T1 and T2. As expected, T1
did increase with increasing field strength but not as
dramatically as might be expected. Subtle differences in the
microstructures of the kidney and the binding of water in
those structures were discernible on the basis of T1
differences at 300 MHz. T2 values decreased, raising
speculation concerning the mechanism for this dependence.
The improved SNR permits smaller samples to be examined at
much higher resolutions (greater than 30 X 30 X 200
microns), further extending the potentials for MR
microscopy.},
Doi = {10.1002/mrm.1910110206},
Key = {Dockery89}
}
@article{fds268831,
Author = {Badea, A and Johnson, GA and Jankowsky, JL},
Title = {Remote sites of structural atrophy predict later amyloid
formation in a mouse model of Alzheimer's
disease.},
Journal = {Neuroimage},
Volume = {50},
Number = {2},
Pages = {416-427},
Year = {2010},
Month = {April},
ISSN = {1095-9572},
url = {http://www.ncbi.nlm.nih.gov/pubmed/20035883},
Keywords = {Alzheimer Disease • Amyloid • Animals •
Atrophy • Brain • Disease Models, Animal •
Female • Image Interpretation, Computer-Assisted •
Magnetic Resonance Imaging • Male • Mice •
Mice, Transgenic • Plaque, Amyloid • metabolism
• methods* • pathology • pathology*},
Abstract = {Magnetic resonance (MR) imaging can provide a longitudinal
view of neurological disease through repeated imaging of
patients at successive stages of impairment. Until recently,
the difficulty of manual delineation has limited volumetric
analyses of MR data sets to a few select regions and a small
number of subjects. Increased throughput offered by faster
imaging methods, automated segmentation, and
deformation-based morphometry have recently been applied to
overcome this limitation with mouse models of neurological
conditions. We use automated analyses to produce an unbiased
view of volumetric changes in a transgenic mouse model for
Alzheimer's disease (AD) at two points in the progression of
disease: immediately before and shortly after the onset of
amyloid formation. In addition to the cortex and
hippocampus, where atrophy has been well documented in AD
patients, we identify volumetric losses in the pons and
substantia nigra where neurodegeneration has not been
carefully examined. We find that deficits in cortical volume
precede amyloid formation in this mouse model, similar to
presymptomatic atrophy seen in patients with familial AD.
Unexpectedly, volumetric losses identified by MR outside of
the forebrain predict locations of future amyloid formation,
such as the inferior colliculus and spinal nuclei, which
develop pathology at very late stages of disease. Our work
provides proof-of-principle that MR microscopy can expand
our view of AD by offering a complete and unbiased
examination of volumetric changes that guide us in
revisiting the canonical neuropathology.},
Language = {eng},
Doi = {10.1016/j.neuroimage.2009.12.070},
Key = {fds268831}
}
@booklet{Tenbrink88,
Author = {R. E. Tenbrink and D. T. Pals and D. W. Harris and G. A.
Johnson},
Title = {Renin inhibitors containing psi-[ch2o] pseudopeptide
inserts},
Journal = {Journal Of Medicinal Chemistry},
Volume = {31},
Number = {3},
Pages = {671 -- 677},
Year = {1988},
Month = {March},
Key = {Tenbrink88}
}
@article{fds174116,
Author = {RE TenBrink and DT Pals and DW Harris and GA Johnson},
Title = {Renin inhibitors containing psi[CH2O] pseudopeptide
inserts.},
Journal = {Journal of medicinal chemistry},
Volume = {31},
Number = {3},
Pages = {671-7},
Year = {1988},
Month = {March},
ISSN = {0022-2623},
Keywords = {Amino Acid Sequence • Angiotensinogen • Chemistry,
Physical • Humans • Molecular Sequence Data •
Physicochemical Phenomena • Renin •
Structure-Activity Relationship • analogs &
derivatives* • antagonists & inhibitors* •
chemical synthesis • pharmacology},
Abstract = {Renin inhibitors 2-4 with the D-Lys renin inhibitory peptide
(RIP) sequence, but containing Leu psi[CH2O]Ala (2), Leu
psi[CH2O]Val (3), and Leu psi[CH2O]Leu (4) at the P1-P1'
site, were of a comparable potency to RIP. N-Terminal
Boc-protected inhibitors containing Pro psi[CH2O]Phe in
positions P4-P3 were potent inhibitors of renin, with
Boc-Phe-Pro psi[CH2O]Phe-His-Leu psi[CH(OH)CH2]Val-Ile-(2-aminomethyl)
pyridine (17) having an IC50 of 1.6 X 10(-9)
M.},
Language = {eng},
Key = {fds174116}
}
@booklet{Scoggins91,
Author = {J. R. Scoggins and J. Arellano and B. Esposito and G. A.
Johnson and K. C. Brundidge},
Title = {Report on a symposium on air-sea interaction and air-mass
modification over the gulf of mexico 7-9 january 1991,
galveston, texas},
Journal = {Bulletin Of The American Meteorological Society},
Volume = {72},
Number = {6},
Pages = {827 -- 832},
Year = {1991},
Month = {June},
Key = {Scoggins91}
}
@article{fds174114,
Author = {RS Dhariwal and GA Johnson and RM Browne and SL Rowles},
Title = {Reprecipitation phenomena arising during the preparation of
demineralised sections. III Scanning electron microscopic
examinations of secondary calcium phosphate
deposits.},
Journal = {Stain technology},
Volume = {51},
Number = {6},
Pages = {293-9},
Year = {1976},
Month = {November},
ISSN = {0038-9153},
Keywords = {Calcium Phosphates • Decalcification Technique* •
Dentin • Humans • Microradiography •
Microscopy, Electron, Scanning • Tooth • Tooth
Calcification* • X-Ray Diffraction • radiography
• ultrastructure*},
Abstract = {Sections of teeth partly demineralized in 10% formic acid
were examined by X-ray diffraction, microradiography and
scanning electron microscopy. In the undemineralized
circumpulpal dentin, the tubules were empty, lying in a
matrix containing hydroxyapatite. In the "plume" areas of
remineralisation, the tubules were filled with mineral
deposits. X-ray diffraction revealed the presence of
brushite and monetite in these areas. In the outer layers of
dentin the tubules were empty, lying in a matrix containing
some residual hydroxyapatite. These findings confirmed that
the remineralisation process occurred within the dentinal
tubules.},
Language = {eng},
Key = {fds174114}
}
@article{fds157079,
Author = {MW Tengowski and P Sutovsky and LW Hedlund and DJ Guyot and JE
Burkhardt, WE Thompson and M Sutovsky and GA Johnson},
Title = {Reproductive cytotoxicity is predicted by magnetic resonance
microscopy and confirmed by ubiquitin-proteasome
immunohistochemistry in a theophylline-induced model of rat
testicular and epididymal toxicity.},
Journal = {Microscopy and microanalysis : the official journal of
Microscopy Society of America, Microbeam Analysis Society,
Microscopical Society of Canada, United States},
Volume = {11},
Number = {4},
Pages = {300-12},
Year = {2005},
Month = {August},
ISSN = {1431-9276},
Keywords = {Animals • Apoptosis • Body Weight •
Epididymis • Immunohistochemistry • In Situ
Nick-End Labeling • Magnetic Resonance Spectroscopy
• Male • Microscopy • Organ Size •
Proteasome Endopeptidase Complex • Rats • Rats,
Sprague-Dawley • Testis • Theophylline •
Ubiquitin • chemistry • drug effects • drug
effects* • metabolism* • physiology* •
toxicity*},
Abstract = {This study investigated the testicular changes in the rat
induced by the nonspecific phosphodiesterase inhibitor,
theophylline using magnetic resonance microscopy (MRM) and
ubiquitin immunostaining techniques. In vivo T1- and
T2-weighted images were acquired at 2 T under anesthesia.
Increased signal observed in the theophylline-treated rats
suggests that leakage of MRM contrast was occurring. In vivo
MRM results indicate that day 16 testis displayed an
increased T1-weighted water signal in the area of the
seminiferous tubule that decreased by day 32. These findings
were validated by histopathology, suggesting that in vivo
MRM has the sensitivity to predict changes in testis and
epididymal tissues. The participation of the ubiquitin
system was investigated, using probes for various markers of
the ubiquitin-proteasome pathway. MRM can be used to detect
subtle changes in the vascular perfusion of organ systems,
and the up-regulation/mobilization of ubiquitin-proteasome
pathway may be one of the mechanisms used in
theophylline-treated epididymis to remove damaged cells
before storage in the cauda epididymis. The combined use of
in vivo MRM and subsequent tissue or seminal analysis for
the presence of ubiquitin in longitudinal studies may become
an important biomarker for assessing testis toxicities drug
studies.},
Key = {fds157079}
}
@article{8754834,
Author = {Tengowski, MW and Sutovsky, P and Hedlund, LW and Guyot, DJ and Burkhardt, JE and Thompson, WE and Sutovsky, M and Johnson,
GA},
Title = {Reproductive cytotoxicity is predicted by magnetic resonance
microscopy and confirmed by ubiquitin-proteasome
immunohistochemistry in a theophylline-induced model of rat
testicular and epididymal toxicity.},
Journal = {Microsc Microanal},
Volume = {11},
Number = {4},
Pages = {300-312},
Year = {2005},
Month = {August},
ISSN = {1431-9276},
url = {http://www.ncbi.nlm.nih.gov/pubmed/16079014},
Keywords = {biochemistry;biological tissues;biomedical MRI;cellular
biophysics;drugs;haemorheology;molecular
biophysics;proteins;},
Abstract = {This study investigated the testicular changes in the rat
induced by the nonspecific phosphodiesterase inhibitor,
theophylline using magnetic resonance microscopy (MRM) and
ubiquitin immunostaining techniques. In vivo T1- and
T2-weighted images were acquired at 2 T under anesthesia.
Increased signal observed in the theophylline-treated rats
suggests that leakage of MRM contrast was occurring. In vivo
MRM results indicate that day 16 testis displayed an
increased T1-weighted water signal in the area of the
seminiferous tubule that decreased by day 32. These findings
were validated by histopathology, suggesting that in vivo
MRM has the sensitivity to predict changes in testis and
epididymal tissues. The participation of the ubiquitin
system was investigated, using probes for various markers of
the ubiquitin-proteasome pathway. MRM can be used to detect
subtle changes in the vascular perfusion of organ systems,
and the up-regulation/mobilization of ubiquitin-proteasome
pathway may be one of the mechanisms used in
theophylline-treated epididymis to remove damaged cells
before storage in the cauda epididymis. The combined use of
in vivo MRM and subsequent tissue or seminal analysis for
the presence of ubiquitin in longitudinal studies may become
an important biomarker for assessing testis toxicities drug
studies.},
Doi = {10.1017/S143192760505021X},
Key = {8754834}
}
@article{fds174170,
Author = {GA Johnson},
Title = {Research into psychiatric disorder after stroke: the need
for further studies.},
Journal = {The Australian and New Zealand journal of
psychiatry},
Volume = {25},
Number = {3},
Pages = {358-70},
Year = {1991},
Month = {September},
ISSN = {0004-8674},
Keywords = {Cerebrovascular Disorders • Delirium, Dementia,
Amnestic, Cognitive Disorders • Depressive Disorder
• Follow-Up Studies • Humans • Sick Role*
• Social Environment • psychology*},
Abstract = {Attention is drawn to some shortcomings of previous findings
with regard to the nature, prevalence and aetiology of
psychiatric disorder after stroke, and in particular
post-stroke depression. Reasons for and drawbacks of the
emphasis on depression in studies to date are discussed.
Inconsistencies amongst previous findings are examined and
it is suggested that many further studies in the area are
warranted providing methodological difficulties are
addressed adequately.},
Language = {eng},
Key = {fds174170}
}
@booklet{Johnson91,
Author = {G. A. Johnson},
Title = {Research into psychiatric-disorder after stroke - the need
for further-studies},
Journal = {Australian And New Zealand Journal Of Psychiatry},
Volume = {25},
Number = {3},
Pages = {358 -- 370},
Year = {1991},
Month = {September},
Key = {Johnson91}
}
@article{fds362951,
Author = {Crater, S and Maharjan, S and Qi, Y and Zhao, Q and Cofer, G and Cook, JC and Johnson, GA and Wang, N},
Title = {Resolution and b value dependent structural connectome in ex
vivo mouse brain.},
Journal = {Neuroimage},
Volume = {255},
Pages = {119199},
Year = {2022},
Month = {July},
url = {http://dx.doi.org/10.1016/j.neuroimage.2022.119199},
Abstract = {Diffusion magnetic resonance imaging has been widely used in
both clinical and preclinical studies to characterize tissue
microstructure and structural connectivity. The diffusion
MRI protocol for the Human Connectome Project (HCP) has been
developed and optimized to obtain high-quality,
high-resolution diffusion MRI (dMRI) datasets. However, such
efforts have not been fully explored in preclinical studies,
especially for rodents. In this study, high quality dMRI
datasets of mouse brains were acquired at 9.4T system from
two vendors. In particular, we acquired a high-spatial
resolution dMRI dataset (25 μm isotropic with 126
diffusion encoding directions), which we believe to be the
highest spatial resolution yet obtained; and a high-angular
resolution dMRI dataset (50 μm isotropic with 384
diffusion encoding directions), which we believe to be the
highest angular resolution compared to the dMRI datasets at
the microscopic resolution. We systematically investigated
the effects of three important parameters that affect the
final outcome of the connectome: b value (1000s/mm2 to
8000 s/mm2), angular resolution (10 to 126), and spatial
resolution (25 µm to 200 µm). The stability of
tractography and connectome increase with the angular
resolution, where more than 50 angles is necessary to
achieve consistent results. The connectome and quantitative
parameters derived from graph theory exhibit a linear
relationship to the b value (R2 > 0.99); a single-shell
acquisition with b value of 3000 s/mm2 shows comparable
results to the multi-shell high angular resolution dataset.
The dice coefficient decreases and both false positive rate
and false negative rate gradually increase with coarser
spatial resolution. Our study provides guidelines and
foundations for exploration of tradeoffs among acquisition
parameters for the structural connectome in ex vivo mouse
brain.},
Doi = {10.1016/j.neuroimage.2022.119199},
Key = {fds362951}
}
@booklet{Johnson87c,
Author = {JOHNSON, GA},
Title = {RESOLUTION LIMITS IN MR - MR MICROSCOPY},
Journal = {MEDICAL PHYSICS},
Volume = {14},
Number = {3},
Pages = {499-499},
Publisher = {AMER INST PHYSICS},
Year = {1987},
Month = {May},
ISSN = {0094-2405},
url = {http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:A1987H776800274&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=47d3190e77e5a3a53558812f597b0b92},
Key = {Johnson87c}
}
@article{fds369118,
Author = {Tian, Y and Cook, JJ and Johnson, GA},
Title = {Restoring morphology of light sheet microscopy data based on
magnetic resonance histology.},
Journal = {Front Neurosci},
Volume = {16},
Pages = {1011895},
Year = {2022},
url = {http://dx.doi.org/10.3389/fnins.2022.1011895},
Abstract = {The combination of cellular-resolution whole brain light
sheet microscopy (LSM) images with an annotated atlas
enables quantitation of cellular features in specific brain
regions. However, most existing methods register LSM data
with existing canonical atlases, e.g., The Allen Brain Atlas
(ABA), which have been generated from tissue that has been
distorted by removal from the skull, fixation and physical
handling. This limits the accuracy of the regional
morphologic measurement. Here, we present a method to
combine LSM data with magnetic resonance histology (MRH) of
the same specimen to restore the morphology of the LSM
images to the in-skull geometry. Our registration pipeline
which maps 3D LSM big data (terabyte per dataset) to MRH of
the same mouse brain provides registration with low
displacement error in ∼10 h with limited manual input. The
registration pipeline is optimized using multiple stages of
transformation at multiple resolution scales. A three-step
procedure including pointset initialization, automated ANTs
registration with multiple optimized transformation stages,
and finalized application of the transforms on
high-resolution LSM data has been integrated into a simple,
structured, and robust workflow. Excellent agreement has
been seen between registered LSM data and reference MRH data
both locally and globally. This workflow has been applied to
a collection of datasets with varied combinations of MRH
contrasts from diffusion tensor images and LSM with varied
immunohistochemistry, providing a routine method for
streamlined registration of LSM images to MRH. Lastly, the
method maps a reduced set of the common coordinate framework
(CCFv3) labels from the Allen Brain Atlas onto the
geometrically corrected full resolution LSM data. The
pipeline maintains the individual brain morphology and
allows more accurate regional annotations and measurements
of volumes and cell density.},
Doi = {10.3389/fnins.2022.1011895},
Key = {fds369118}
}
@booklet{Johnson84a,
Author = {G. A. Johnson},
Title = {Retrofit control of a variable air volume system using
variable speed drives},
Journal = {Ashrae Journal-american Society Of Heating Refrigerating And
Air-conditioning Engineers},
Volume = {26},
Number = {5},
Pages = {44 -- 44},
Year = {1984},
Key = {Johnson84a}
}
@booklet{Hoverstad04,
Author = {T. R. Hoverstad and J. L. Gunsolus and G. A. Johnson and R.
R. King},
Title = {Risk-efficiency criteria for evaluating economics of
herbicide-based weed management systems in
corn},
Journal = {Weed Technology},
Volume = {18},
Number = {3},
Pages = {687 -- 697},
Year = {2004},
Key = {Hoverstad04}
}
@article{fds268708,
Author = {Clark, DP and Johnson, GA and Badea, CT},
Title = {Robust material decomposition for spectral
CT},
Journal = {Progress in Biomedical Optics and Imaging - Proceedings of
SPIE},
Volume = {9038},
Publisher = {SPIE},
Year = {2014},
Month = {January},
ISBN = {9780819498311},
ISSN = {1605-7422},
url = {http://dx.doi.org/10.1117/12.2042546},
Abstract = {There is ongoing interest in extending CT from anatomical to
functional imaging. Recent successes with dual energy CT,
the introduction of energy discriminating x-ray detectors,
and novel, target-specific, nanoparticle contrast agents
enable functional imaging capabilities via spectral CT.
However, many challenges related to radiation dose, photon
flux, and sensitivity still must be overcome. Here, we
introduce a post-reconstruction algorithm called spectral
diffusion that performs a robust material decomposition of
spectral CT data in the presence of photon noise to address
these challenges. Specifically, we use spectrally joint,
piece-wise constant kernel regression and the split Bregman
method to iteratively solve for a material decomposition
which is gradient sparse, quantitatively accurate, and
minimally biased relative to the source data. Spectral
diffusion integrates structural information from multiple
spectral channels and their corresponding material
decompositions within the framework of diffusion-like
denoising algorithms. Using a 3D, digital bar phantom and a
material sensitivity matrix calibrated for use with a
polychromatic x-ray source, we quantify the limits of
detectability (CNR = 5) afforded by spectral diffusion in
the triple-energy material decomposition of iodine (3.1
mg/mL), gold (0.9 mg/mL), and gadolinium (2.9 mg/mL)
concentrations. © 2014 SPIE.},
Doi = {10.1117/12.2042546},
Key = {fds268708}
}
@booklet{Johnson00,
Author = {G. A. Johnson and F. W. Bazer and L. A. Jaeger and T. E.
Spencer and C. Pfarrer and R. C. Burghardt},
Title = {Role of MUC-1, integrins and extracellular matrix components
in the implantation cascade in sheep.},
Journal = {Biology Of Reproduction},
Volume = {62},
Pages = {281 -- 282},
Year = {2000},
Key = {Johnson00}
}
@booklet{Garlow00,
Author = {J. E. Garlow and H. H. Ka and G. A. Johnson and L. A. Jaeger and R. C. Burghardt and F. W. Bazer},
Title = {Role of osteopontin during early pregnancy in
pigs.},
Journal = {Biology Of Reproduction},
Volume = {62},
Pages = {282 -- 282},
Year = {2000},
Key = {Garlow00}
}
@article{fds204266,
Author = {KJ Bayless and GA Johnson},
Title = {Role of the cytoskeleton in formation and maintenance of
angiogenic sprouts.},
Journal = {Journal of vascular research},
Volume = {48},
Number = {5},
Pages = {369-85},
Year = {2011},
ISSN = {1423-0135},
url = {http://dx.doi.org/10.1159/000324751},
Keywords = {Animals • Cytoskeleton • Humans •
Neovascularization, Physiologic • Wound Healing •
physiology*},
Abstract = {Angiogenesis is the formation of new blood vessels from
pre-existing structures, and is a key step in tissue and
organ development, wound healing and pathological events.
Changes in cell shape orchestrated by the cytoskeleton are
integral to accomplishing the various steps of angiogenesis,
and an intact cytoskeleton is also critical for maintaining
newly formed structures. This review focuses on how the 3
main cytoskeletal elements--microfilaments, microtubules,
and intermediate filaments--regulate the formation and
maintenance of angiogenic sprouts. Multiple classes of
compounds target microtubules and microfilaments, revealing
much about the role of actin and tubulin and their
associated molecules in angiogenic sprout formation and
maintenance. In contrast, intermediate filaments are much
less studied, yet intriguing evidence suggests a vital, but
unresolved, role in angiogenic sprouting. This review
discusses evidence for regulatory molecules and
pharmacological compounds that affect actin, microtubule and
intermediate filament dynamics to alter various steps of
angiogenesis, including endothelial sprout formation and
maintenance.},
Language = {eng},
Doi = {10.1159/000324751},
Key = {fds204266}
}
@booklet{Stewart02,
Author = {M. D. Stewart and Y. S. Choi and G. A. Johnson and L. Y.
Yu-lee and F. W. Bazer and T. E. Spencer},
Title = {Roles of Stat1, Stat2, and interferon regulatory factor-9
(IRF-9) in interferon tau regulation of IRF-1},
Journal = {Biology Of Reproduction},
Volume = {66},
Number = {2},
Pages = {393 -- 400},
Year = {2002},
Month = {February},
Key = {Stewart02}
}
@article{fds174192,
Author = {MD Stewart and Y Choi and GA Johnson and LY Yu-Lee and FW Bazer and TE
Spencer},
Title = {Roles of Stat1, Stat2, and interferon regulatory factor-9
(IRF-9) in interferon tau regulation of IRF-1.},
Journal = {Biology of reproduction},
Volume = {66},
Number = {2},
Pages = {393-400},
Year = {2002},
Month = {February},
ISSN = {0006-3363},
Keywords = {Blotting, Western • Cell Line • Cells, Cultured
• DNA-Binding Proteins • Fibroblasts • Humans
• Indicators and Reagents • Interferon Regulatory
Factor-1 • Interferon Type I •
Interferon-Stimulated Gene Factor 3 •
Interferon-Stimulated Gene Factor 3, gamma Subunit •
Phosphoproteins • Phosphorylation • Pregnancy
Proteins • RNA, Messenger • Reverse Transcriptase
Polymerase Chain Reaction • STAT1 Transcription Factor
• STAT2 Transcription Factor • Signal Transduction
• Trans-Activators • Transcription Factors •
Tyrosine • biosynthesis • genetics •
metabolism • physiology • physiology*},
Abstract = {Interferon tau (IFNtau) is the pregnancy recognition signal
produced by the conceptus trophectoderm and acts in a
paracine manner on the ovine endometrium to increase
expression of IFN-stimulated genes primarily in the stroma
and deep glandular epithelium, including IFN regulatory
factor-1 (IRF-1). The roles of Stat1, Stat2, and IRF-9 in
IFNtau regulation of IRF-1 expression were determined using
human stromal fibroblasts lacking specific IFN signaling
components or complemented with specific Stat1 mutants. In
parental (2fTGH) cells treated with IFNtau, Stat1alpha/beta
was tyrosine phosphorylated by 15 min, and IRF-1 mRNA and
protein increased from 0 to 6 h, was maximal at 6 h, and
decreased to 24 h. In contrast, IFNtau did not affect IRF-1
expression in Stat1- and Stat2-deficient cells or in
Stat1-deficient cells complemented with Stat1 Y701Q or Stat1
R602L mutants. In Stat1-deficient cells complemented with
the Stat1 S727A mutant, Stat1alpha, or Stat1beta and treated
with IFNtau, IRF-1 increased from 0 to 6 h, was maximal at 6
h, and decreased thereafter. In IRF-9-deficient cells
stimulated with IFNtau, IRF-1 increased from 0 to 6 h but
did not exhibit the sharp decline from 6 to 12 h observed in
other cells. Collectively, results indicate that IFNtau
effect on IRF-1 expression is primarily regulated by
tyrosine-phosphorylated Stat1alpha or Stat1beta dimers,
whereas the decline of IRF-1 after 6 h of IFNtau treatment
is regulated by IRF-9.},
Language = {eng},
Key = {fds174192}
}
@booklet{Mcfall97,
Author = {McFall, JS and Johnson, GA},
Title = {Root form and function meshed by MRI.},
Journal = {PLANT PHYSIOLOGY},
Volume = {114},
Number = {3},
Pages = {20002-20002},
Year = {1997},
ISSN = {0032-0889},
url = {http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:A1997XL11900006&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=47d3190e77e5a3a53558812f597b0b92},
Key = {Mcfall97}
}
@article{06229906637,
Author = {Miller, D.R. and Chen, S.Y. and Porter, P.M. and Johnson,
G.A. and Wyse, D.L. and Stetina, S.R. and Klossner, L.D. and Nelson, G.A.},
Title = {Rotation crop evaluation for management of the soybean cyst
Nematode in Minnesota},
Journal = {Agronomy Journal},
Volume = {98},
Number = {3},
Pages = {569 - 578},
Year = {2006},
url = {http://dx.doi.org/10.2134/agronj2005.0185},
Keywords = {Cultivation;},
Abstract = {Crop rotation is an effective tactic for soybean cyst
nematode (SCN) management. In the North Central region of
the USA, corn is almost exclusively used as a nonhost
rotation crop with soybean. This study was conducted to
determine the effectiveness of crops common to or having
potential use in the North Central region as rotation crops
for managing SCN. Sixteen potential rotation crops and
SCN-resistant and susceptible soybeans were grown along with
six fallow controls in three commercial field sites near
Waseca, Lamberton, and Morris, MN, in 2001, and
SCN-susceptible soybean was grown on all plots in 2002.
Nematode populations at planting, midseason, and harvest
were measured both years; soybean yield was measured in
2002. There was large variability in SCN populations and
soybean yields at the three sites. Nevertheless, significant
treatment effects were detected at all sites. While all of
the rotation crops lowered SCN populations compared with
SCN-susceptible soybean, there were only subtle differences
among the individual rotation crops and among different
groups of the crops. Leguminous nonhosts or poor hosts were
best in reducing SCN population density. Corn, the most
common rotation crop in Minnesota, was among the least
effective in reducing nematode populations. There was an
undetectable yield benefit from SCN management, although
differences in yield were observed among the rotation crop
treatments-probably due to agronomic factors. The data
suggest that a single year of rotation of soybean with any
of these crops before planting a susceptible soybean may not
be sufficient in managing SCN. © American Society of
Agronomy.},
Key = {06229906637}
}
@article{fds132737,
Author = {G.A. Johnson},
Title = {S. Hurlston, R.D. Black, W. Brey, X.J. Chen, L.W. Hedlund,
R. Withers, M.Yap, G.A. Johnson. A superconducting
surface coil for in vivo MR microscopy. in "Proc., SMR 4th
Annual Scientific Meeting. New York, NY, 1996,"
p.129.},
Year = {1996},
Key = {fds132737}
}
@article{fds371721,
Author = {Johnson, G and Stubbs, WA and Woods, HF and Alberti,
KGMM},
Title = {Salicylate induced hypoglycaemia: a possible
mechanism},
Journal = {European Journal of Clinical Investigation},
Volume = {4},
Number = {5},
Year = {1974},
Month = {January},
Key = {fds371721}
}
@booklet{Johnson80e,
Author = {JOHNSON, GA and RAVIN, CE},
Title = {SCATTERED RADIATION FROM THE FILM HOLDER IN CHEST
RADIOGRAPHY},
Journal = {AMERICAN JOURNAL OF ROENTGENOLOGY},
Volume = {134},
Number = {1},
Pages = {201-201},
Publisher = {AMER ROENTGEN RAY SOC},
Year = {1980},
Month = {January},
ISSN = {0361-803X},
url = {http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:A1980HZ88000054&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=47d3190e77e5a3a53558812f597b0b92},
Key = {Johnson80e}
}
@article{fds174177,
Author = {FJ White and RC Burghardt and J Hu and MM Joyce and TE Spencer and GA
Johnson},
Title = {Secreted phosphoprotein 1 (osteopontin) is expressed by
stromal macrophages in cyclic and pregnant endometrium of
mice, but is induced by estrogen in luminal epithelium
during conceptus attachment for implantation.},
Journal = {Reproduction (Cambridge, England)},
Volume = {132},
Number = {6},
Pages = {919-29},
Year = {2006},
Month = {December},
ISSN = {1470-1626},
url = {http://dx.doi.org/10.1530/REP-06-0068},
Keywords = {Animals • Embryo Implantation • Endometrium •
Epithelium • Estrogens • Estrous Cycle •
Female • Fluorescent Antibody Technique • In Situ
Hybridization • Leukocytes • Macrophages •
Mice • Osteopontin • Ovariectomy • Placenta
• Pregnancy • Pregnancy, Animal •
Progesterone • RNA, Messenger • analysis •
chemistry • genetics • immunology •
metabolism • metabolism* • pharmacology •
physiology* • secretion*},
Abstract = {Secreted phosphoprotein 1 (SPP1, osteopontin) is the most
highly upregulated extracellular matrix/adhesion
molecule/cytokine in the receptive phase human uterus, and
Spp1 null mice manifest decreased pregnancy rates during
mid-gestation as compared with wild-type counterparts. We
hypothesize that Spp1 is required for proliferation,
migration, survival, adhesion, and remodeling of cells at
the conceptus-maternal interface. Our objective was to
define the temporal/spatial distribution and steroid
regulation of Spp1 in mouse uterus during estrous cycle and
early gestation. In situ hybridization localized Spp1 to
luminal epithelium (LE) and immune cells. LE expression was
prominent at proestrus, decreased by estrus, and was nearly
undetectable at diestrus. During pregnancy, Spp1 mRNA was
not detected in LE until day 4.5 (day 1 = vaginal plug).
Spp1-expressing immune cells were scattered within the
endometrial stroma throughout the estrous cycle and early
pregnancy. Immunoreactive Spp1 was prominent at the apical
LE surface by day 4.5 of pregnancy and Spp1 protein was also
co-localized with subsets of CD45-positive (leukocytes) and
F4/80-positive (macrophages) cells. In ovariectomized mice,
estrogen, but not progesterone, induced Spp1 mRNA, whereas
estrogen plus progesterone did not induce Spp1 in LE. These
results establish that estrogen regulates Spp1 in mouse LE
and are the first to identify macrophages that produce Spp1
within the peri-implantation endometrium of any species. We
suggest that Spp1 at the apical surface of LE provides a
mechanism to bridge conceptus to LE during implantation, and
that Spp1-positive macrophages within the stroma may be
involved in uterine remodeling for conceptus
invasion.},
Language = {eng},
Doi = {10.1530/REP-06-0068},
Key = {fds174177}
}
@article{fds174070,
Author = {DW Erikson and RC Burghardt and KJ Bayless and GA
Johnson},
Title = {Secreted phosphoprotein 1 (SPP1, osteopontin) binds to
integrin alpha v beta 6 on porcine trophectoderm cells and
integrin alpha v beta 3 on uterine luminal epithelial cells,
and promotes trophectoderm cell adhesion and
migration.},
Journal = {Biology of reproduction},
Volume = {81},
Number = {5},
Pages = {814-25},
Year = {2009},
Month = {November},
ISSN = {1529-7268},
url = {http://dx.doi.org/10.1095/biolreprod.109.078600},
Keywords = {Animals • Antigens, Neoplasm • Cattle • Cell
Adhesion • Cell Line • Cell Movement • Cell
Proliferation • Chromatography, Affinity •
Dose-Response Relationship, Drug • Embryo Implantation
• Epithelial Cells • Extracellular Matrix •
Female • Fluorescent Antibody Technique • Integrin
alphaVbeta3 • Integrins • Osteopontin •
Pregnancy • Rats • Swine • Uterus • drug
effects • metabolism • metabolism* •
pharmacology • physiology*},
Abstract = {Conceptus implantation involves pregnancy-specific
alterations in extracellular matrix at the
conceptus-maternal interface. Secreted phosphoprotein 1
(SPP1, osteopontin) is induced just before implantation and
is present at the conceptus-maternal interface in mammals.
In the present study, we investigated mechanisms by which
SPP1 facilitates porcine conceptus and uterine luminal
epithelial cell attachment. Native bovine milk and wild-type
rat recombinant SPP1 stimulated trophectoderm cell
migration. Bovine milk SPP1, ovine uterine SPP1, and
recombinant wild-type, but not mutated, rat SPP1 promoted
dose- and cation-dependent attachment of porcine
trophectoderm and uterine luminal epithelial cells, which
was markedly reduced in the presence of a linear Arg-Gly-Asp
integrin-blocking peptide. Affinity chromatography and
immunoprecipitation experiments revealed direct binding of
alpha v beta 6 trophectoderm and alpha v beta 3 uterine
epithelial cell integrins to SPP1. Immunofluorescence
microscopy using SPP1-coated microspheres revealed
colocalization of the alpha v integrin subunit and talin at
focal adhesions as well as at the apical domain of
trophectoderm cells. Similarly, immunofluorescence staining
of implantation sites in frozen gravid uterine cross
sections localized SPP1 and alpha v integrin to the apical
surfaces of trophectoderm and luminal epithelium and beta 3
integrin to the apical surface of luminal epithelium. To our
knowledge, the present study is the first to demonstrate
functionally that SPP1 directly binds specific integrins to
promote trophectoderm cell migration and attachment to
luminal epithelium that may be critical to conceptus
elongation and implantation.},
Language = {eng},
Doi = {10.1095/biolreprod.109.078600},
Key = {fds174070}
}
@article{fds174171,
Author = {J Kim and DW Erikson and RC Burghardt and TE Spencer and G Wu and KJ
Bayless, GA Johnson and FW Bazer},
Title = {Secreted phosphoprotein 1 binds integrins to initiate
multiple cell signaling pathways, including FRAP1/mTOR, to
support attachment and force-generated migration of
trophectoderm cells.},
Journal = {Matrix biology : journal of the International Society for
Matrix Biology},
Year = {2010},
Month = {April},
ISSN = {1569-1802},
url = {http://dx.doi.org/10.1016/j.matbio.2010.04.001},
Abstract = {Attachment and migration of trophectoderm (Tr) cells,
hallmarks of blastocyst implantation in mammals, are unique
uterine events. Secreted phosphoprotein 1 (SPP1) in the
uterus binds integrins on conceptus Tr and uterine luminal
epithelium (LE), affecting cell-cell and cell-matrix
interactions. The signal transduction pathways activated by
SPP1 and integrins in conceptuses have not been elucidated.
Results of this study demonstrate that SPP1 binds
alphavbeta3 and alpha5beta1 integrins to induce focal
adhesion assembly, a prerequisite for adhesion and migration
of Tr, through activation of: 1) P70S6K via crosstalk
between FRAP1/mTOR and MAPK pathways; 2) mTOR, PI3K,
MAPK3/MAPK1 (Erk1/2) and MAPK14 (p38) signaling to stimulate
Tr cell migration; and 3) focal adhesion assembly and myosin
II motor activity to induce migration of Tr cells. These
cell signaling pathways, acting in concert, mediate
adhesion, migration and cytoskeletal remodeling of Tr cells
essential for expansion and elongation of conceptuses and
attachment to uterine LE for implantation.},
Language = {ENG},
Doi = {10.1016/j.matbio.2010.04.001},
Key = {fds174171}
}
@article{fds174298,
Author = {FW Bazer and H Gao and GA Johnson and G Wu and DW Bailey and RC
Burghardt},
Title = {Select nutrients and glucose transporters in pig uteri and
conceptuses.},
Journal = {Society of Reproduction and Fertility supplement},
Volume = {66},
Pages = {335-6},
Year = {2009},
Keywords = {Amino Acids • Animals • Arginine • Embryo,
Mammalian • Estradiol • Female • Glucose
• Glucose Transport Proteins, Facilitative •
Glutamine • Leucine • Pregnancy • Pregnancy,
Animal • Pseudopregnancy • Swine • Uterus
• analogs & derivatives • chemically induced
• metabolism • metabolism*},
Language = {eng},
Key = {fds174298}
}
@article{fds174210,
Author = {MC Satterfield and H Gao and X Li and G Wu and GA Johnson and TE Spencer and FW Bazer},
Title = {Select nutrients and their associated transporters are
increased in the ovine uterus following early progesterone
administration.},
Journal = {Biology of reproduction},
Volume = {82},
Number = {1},
Pages = {224-31},
Year = {2010},
Month = {January},
ISSN = {1529-7268},
url = {http://dx.doi.org/10.1095/biolreprod.109.076729},
Keywords = {Amino Acids • Animals • Blastocyst • Cationic
Amino Acid Transporter 2 • Embryonic Development*
• Endometrium • Female • Glucose •
Glucose Transporter Type 1 • Mifepristone •
Progesterone • RNA, Messenger • Sheep •
Sodium-Glucose Transporter 1 • metabolism •
metabolism* • pharmacology • pharmacology* •
physiology*},
Abstract = {The intrauterine milieu is a complex mixture of substances
originating from serum and endometrium that support
blastocyst growth and development. The present study
identified alterations in glucose and amino acids in
response to an early rise in progesterone (P4), which
accelerates blastocyst growth and development. Bred ewes
received daily injections of either corn oil (CO) vehicle or
P4 from 36 h postmating (Day 0) to either Day 9 or Day 12.
Another group of ewes received P4 to Day 8 and the
antiprogestin mifepristone (RU486) from Day 8 to Day 12. The
total amount of glucose, aspartate (acidic amino acid),
arginine and lysine (basic amino acids), and citrulline,
asparagine, serine, glutamine, beta-alanine, and alanine
(neutral amino acids) was greater in uterine flushings from
early P4- than CO-treated ewes on Day 9. On Day 12, only
arginine and lysine were higher in uterine flushings from
P4-treated ewes, whereas citrulline was reduced. Glucose
transporters, SLC2A1 and SLC5A1, were increased in uterine
luminal (LE) and superficial glandular (sGE) epithelia of
early P4-treated ewes on Days 9 and 12 but were reduced in
endometria from ewes treated with both P4 and RU486 (P4+RU).
SLC7A2B, a transporter of basic amino acids, increased in
LE/sGE of P4- versus CO-treated ewes on Day 12 but was
reduced in P4+RU-treated ewes. Thus, select nutrients are
increased in the uterine lumen by P4 concomitant with the
upregulation of epithelial transporters for glucose and
basic amino acids, suggesting that these nutrients stimulate
blastocyst growth and development.},
Language = {eng},
Doi = {10.1095/biolreprod.109.076729},
Key = {fds174210}
}
@article{fds174215,
Author = {H Gao and G Wu and TE Spencer and GA Johnson and X Li and FW
Bazer},
Title = {Select nutrients in the ovine uterine lumen. I. Amino acids,
glucose, and ions in uterine lumenal flushings of cyclic and
pregnant ewes.},
Journal = {Biology of reproduction},
Volume = {80},
Number = {1},
Pages = {86-93},
Year = {2009},
Month = {January},
ISSN = {0006-3363},
url = {http://dx.doi.org/10.1095/biolreprod.108.071597},
Keywords = {Amino Acids • Animals • Calcium • Estrous
Cycle • Female • Glucose • Glutathione •
Potassium • Pregnancy • Pregnancy, Animal •
Sheep • Sodium • Uterus • metabolism •
metabolism*},
Abstract = {Nutrients in uterine secretions are essential for
development and survival of conceptuses (embryo and
associated extraembryonic membranes) during pregnancy;
however, little is known about changes in the amounts of
specific nutrients in the uterine fluids of cyclic and
pregnant ruminants. This study determined quantities of
glucose, amino acids, glutathione, calcium, sodium, and
potassium in uterine lumenal fluid from cyclic (Days 3-16)
and pregnant (Days 10-16) ewes. Total recoverable glucose,
Arg, Gln, Leu, Asp, Glu, Asn, His, beta-Ala, Tyr, Trp, Met,
Val, Phe, Ile, Lys, Cys, Pro, glutathione, calcium, and
sodium were greater in the uterine fluid of pregnant
compared with cyclic ewes between Days 10 and 16. In cyclic
ewes, only modest changes in the total amounts of glucose,
Asn, Cit, Tyr, Trp, Met, Val, Cys, glutathione, calcium, and
potassium were detected between Days 3 and 16. However, in
pregnant ewes, amounts of glucose, Arg, Gln, Glu, Gly, Cys,
Leu, Pro, glutathione, calcium, and potassium in uterine
fluids increased 3- to 23-fold between Days 10 and 14 and
remained high to Day 16. Of particular interest were
increases in glucose, Arg, Leu, and Gln in uterine flushings
of pregnant ewes between Days 10 and 16 of pregnancy. Total
amounts of His, ornithine, Lys, Ser, Thr, Ile, Phe, Trp,
Met, and Cit in uterine fluids also increased, but to a
lesser extent during early pregnancy. These novel results
indicate activation of pregnancy-associated mechanisms for
transport of nutrients into the uterine lumen, and they
provide a framework for future studies of nutrients,
including glucose, amino acids, and glutathione, required to
activate nutrient-sensing cell signaling pathways for
growth, development, and survival of conceptuses, as well as
for optimization of culture media for in vitro studies of
conceptus development.},
Language = {eng},
Doi = {10.1095/biolreprod.108.071597},
Key = {fds174215}
}
@article{fds174079,
Author = {H Gao and G Wu and TE Spencer and GA Johnson and FW Bazer},
Title = {Select nutrients in the ovine uterine lumen. ii. glucose
transporters in the uterus and peri-implantation
conceptuses.},
Journal = {Biology of reproduction},
Volume = {80},
Number = {1},
Pages = {94-104},
Year = {2009},
Month = {January},
ISSN = {0006-3363},
url = {http://dx.doi.org/10.1095/biolreprod.108.071654},
Keywords = {Animals • Blastocyst • Estrous Cycle • Female
• Gene Expression Regulation, Developmental •
Glucose Transport Proteins, Facilitative •
Immunohistochemistry • In Situ Hybridization •
Interferon Type I • Pregnancy • Pregnancy Proteins
• Progesterone • RNA, Messenger • Random
Allocation • Sheep • Uterus • antagonists &
inhibitors • biosynthesis • genetics •
metabolism* • pharmacology* • physiology •
veterinary},
Abstract = {Total glucose in ovine uterine lumenal fluid increases
6-fold between Days 10 and 15 of gestation, but not the
estrous cycle; however, mechanisms for glucose transport
into the uterine lumen and uptake by conceptuses
(embryo/fetus and associated membranes) are not established.
This study determined the effects of the estrous cycle,
pregnancy, progesterone (P4), and interferon tau (IFNT) on
expression of both facilitative (SLC2A1, SLC2A3, and SLC2A4)
and sodium-dependent (SLC5A1 and SLC5A11) glucose
transporters in ovine uterine endometria from Days 10 to 16
of the estrous cycle and Days 10 to 20 of pregnancy, as well
as in conceptuses from Days 10 to 20 of pregnancy. The
SLC2A1 and SLC5A1 mRNAs and proteins were most abundant in
uterine luminal epithelia and superficial glandular
epithelia (LE/sGE), whereas SLC2A4 was present in stromal
cells and glandular epithelia (GE). SLC5A11 mRNA was most
abundant in endometrial GE, whereas SLC2A3 mRNA was not
detectable in endometria. SLC2A1, SLC2A3, SLC2A4, SLC5A1,
and SLC5A11 were expressed in the trophectoderm and endoderm
of conceptuses. Steady-state levels of SLC2A1, SLC5A1, and
SLC5A11 mRNAs, but not SLC2A4 mRNA, were greater in
endometria from pregnant than from cyclic ewes. Progesterone
increased SLC2A1, SLC5A11, and SLC2A4 mRNAs in the LE/sGE
and SLC5A1 in the GE of ovariectomized ewes. Expression of
SLC5A1 was inhibited by ZK136,317 (progesterone receptor
antagonist), and the combination of ZK136,317 and IFNT
further decreased expression in GE. In constrast, P4 induced
and IFNT stimulated expression of SLC2A1 and SLC5A11, and
these effects were blocked by ZK136,317. Results of this
study indicate differential expression of facilitative and
sodium-dependent glucose transporters in ovine uteri and
conceptuses for transport and uptake of glucose, and that P4
or P4 and IFNT regulate their expression during the
peri-implantation period of pregnancy.},
Language = {eng},
Doi = {10.1095/biolreprod.108.071654},
Key = {fds174079}
}
@article{fds174130,
Author = {H Gao and G Wu and TE Spencer and GA Johnson and FW Bazer},
Title = {Select nutrients in the ovine uterine lumen. III. Cationic
amino acid transporters in the ovine uterus and
peri-implantation conceptuses.},
Journal = {Biology of reproduction},
Volume = {80},
Number = {3},
Pages = {602-9},
Year = {2009},
Month = {March},
ISSN = {0006-3363},
url = {http://dx.doi.org/10.1095/biolreprod.108.073890},
Keywords = {Amino Acid Transport Systems, Basic • Animals •
Arginine • Biological Transport • Blastocyst
• Cationic Amino Acid Transporter 1 • Cationic
Amino Acid Transporter 2 • Endometrium • Estrous
Cycle • Female • Interferon Type I •
Pregnancy • Pregnancy Proteins • Pregnancy, Animal
• Progesterone • RNA, Messenger • Receptors,
Progesterone • Sheep • Uterus • antagonists &
inhibitors • metabolism • metabolism* •
pharmacology • physiology},
Abstract = {Arginine is an essential amino acid for conceptus
(embryo/fetus and trophoblast/placenta) growth and
development; however, the mechanisms for arginine transport
into the uterine lumen and uptake by conceptuses are largely
unknown. In this study, expression of System y(+) (SLC7A1,
SLC7A2, and SLC7A3) cationic amino acid transporters in
uteri of cyclic and pregnant ewes and conceptuses was
studied, and effects of pregnancy, progesterone (P4), and
interferon tau (IFNT) on their expression were investigated.
SLC7A1 mRNA was most abundant in endometrial luminal (LE)
and superficial glandular (sGE) epithelia on Day 16 of the
estrous cycle and on Days 16-20 of pregnancy, whereas SLC7A2
mRNA was most abundant in LE and mid to deep glandular (GE)
epithelia on Days 14-20 of gestation. Expression of SLC7A1
and SLC7A2 was enhanced in pregnant ewes in a cell-specific
manner, but abundance of SLC7A3 was not affected by day of
the estrous cycle or by pregnancy status. SLC7A1, SLC7A2,
and SLC7A3 mRNAs were expressed in trophectoderm and
endoderm of conceptuses. In ovariectomized ewes, short-term
treatment of ewes with P4 and IFNT did not affect
endometrial SLC7A1 mRNA, while long-term treatment with P4
stimulated SLC7A1 in LE and GE, and IFNT tended to increase
SLC7A1 abundance in LE. SLC7A2 mRNA abundance increased
4.1-fold in response to short-term P4 treatment and an
additional 1.7-fold by IFNT primarily in endometrial LE/sGE,
and these effects were ablated by a P4 receptor antagonist.
These results indicate that coordinate changes in SLC7A1,
SLC7A2, and SLC7A3 expression in uterine endometria and
conceptuses are likely important in transport of arginine
that is critical to conceptus growth, development, and
survival.},
Language = {eng},
Doi = {10.1095/biolreprod.108.073890},
Key = {fds174130}
}
@article{fds174094,
Author = {H Gao and G Wu and TE Spencer and GA Johnson and FW Bazer},
Title = {Select nutrients in the ovine uterine lumen. IV. Expression
of neutral and acidic amino acid transporters in ovine uteri
and peri-implantation conceptuses.},
Journal = {Biology of reproduction},
Volume = {80},
Number = {6},
Pages = {1196-208},
Year = {2009},
Month = {June},
ISSN = {0006-3363},
url = {http://dx.doi.org/10.1095/biolreprod.108.075440},
Keywords = {Amino Acid Transport Systems, Acidic • Amino Acid
Transport Systems, Neutral • Animals • Corpus
Luteum • Embryo, Mammalian • Endometrium •
Estrous Cycle • Female • Interferon Type I •
Pregnancy • Pregnancy Proteins • Pregnancy, Animal
• Progesterone • RNA, Messenger • Sheep
• metabolism • metabolism*},
Abstract = {The availability of specific neutral and acidic amino acids
in the uterine lumen of ewes increased significantly during
the peri-implantation period, but mechanisms for their
transport into the uterine lumen and uptake by conceptuses
are not established in any species. In this study, effects
of pregnancy, progesterone (P4), and interferon tau (IFNT)
on expression of neutral and acidic amino acid transporters
in uteri of cyclic and pregnant ewes and conceptuses were
studied. SLC1A2, SLC1A3, SLC3A1, SLC6A14, SLC6A19, SLC7A6,
SLC38A3, and SLC38A6 mRNAs were only weakly expressed in the
ovine endometrium. However, SLC1A4, SLC1A5, SLC7A8, and
SLC43A2 mRNAs were detectable in uterine luminal epithelia
(LE), superficial glandular epithelia (sGE), and/or
glandular epithelia (GE). SLC1A1 and SLC7A5 mRNAs were most
abundant in LE/sGE and GE. SLC1A3 and SLC38A4 mRNAs were
most abundant in uterine stroma. SLC38A6 mRNA was detected
only in cells with a stromal distribution suggesting immune
lineage. SLC1A5 mRNA was expressed primarily in LE/sGE and
stromal cells, and it was more abundant in uteri of pregnant
ewes (day x status interaction; P < 0.05). Furthermore, P4
induced and IFNT further stimulated SLC1A5 expression in
LE/sGE. Endometrial SLC1A1, SLC7A5, and SLC43A2 mRNAs
demonstrated both temporal and cellSLC-specific changes.
Several mRNAs were detectable in trophectoderm (SLC6A19,
SLC7A5, SLC7A6, and SLC43A2), while others were more
abundant in endoderm (SLC1A4, SLC1A5, SLC6A19, SLC7A5,
SLC7A6, SLC7A8, and SLC43A2) of conceptuses. These results
document coordinate changes in expression of transporters
that are likely responsible for increases in amounts of
neutral and acidic amino acids in the uterine lumen to
support conceptus growth, development, and
survival.},
Language = {eng},
Doi = {10.1095/biolreprod.108.075440},
Key = {fds174094}
}
@article{fds204259,
Author = {J Kim and RC Burghardt and G Wu and GA Johnson and TE Spencer and FW
Bazer},
Title = {Select nutrients in the ovine uterine lumen. IX.
Differential effects of arginine, leucine, glutamine, and
glucose on interferon tau, ornithine decarboxylase, and
nitric oxide synthase in the ovine conceptus.},
Journal = {Biology of reproduction},
Volume = {84},
Number = {6},
Pages = {1139-47},
Year = {2011},
Month = {June},
ISSN = {1529-7268},
url = {http://dx.doi.org/10.1095/biolreprod.110.088153},
Keywords = {Amino Acids • Animals • Arginine • Embryo,
Mammalian • Embryonic Development • Female •
Gene Expression Regulation, Developmental • Glucose
• Glutamine • Interferon Type I • Leucine
• Nitric Oxide Synthase • Ornithine Decarboxylase
• Pregnancy • Pregnancy Proteins • RNA,
Messenger • Sheep • Uterus • drug effects
• genetics • metabolism • metabolism* •
pharmacology • pharmacology* • physiology •
physiology*},
Abstract = {Nutrients are primary requirements for development of
conceptuses (embryo and extraembryonic membranes), including
protein synthesis. We have shown that arginine (Arg),
leucine (Leu), and glucose stimulate protein synthesis
through phosphorylation of MTOR signaling molecules, thereby
increasing proliferation of ovine trophectoderm cells. This
study determined whether Arg, Leu, glutamine (Gln), and
glucose influence gene expression and protein synthesis in
explant cultures of ovine conceptuses recovered from ewes on
Day 16 of pregnancy. Conceptuses were deprived of select
nutrients and then cultured with either Arg, Leu, Gln, or
glucose for 18 h, after which they were analyzed for
abundance of MTOR, RPS6K, RPS6, EIF4EBP1 (also known as
4EBP1), IFNT, NOS2, NOS3, GCH1, and ODC1 mRNAs and proteins.
Levels of MTOR, RPS6K, RPS6, and EIF4EBP1 mRNAs were not
affected by treatment with any of the select nutrients.
Similarly, expression of IFNT, NOS2, NOS3, and ODC1 mRNAs
were not different. Interestingly, GCH1 mRNA levels
increased in response to Arg treatment. Importantly, Arg,
Leu, Gln, and glucose increased the abundance of
phosphorylated MTOR, RPS6K, RPS6, and EIF4EBP1 proteins as
well as NOS and ODC1 proteins, but only Arg increased the
abundance of IFNT protein. These findings indicate that Arg,
Leu, Gln, and glucose stimulate translation of mRNAs to
increase synthesis of proteins through phosphorylation and
activation of components of the MTOR signaling pathway.
Increases in abundance of IFNT protein (the pregnancy
recognition signal), NOS2, NOS3 and GCH1 for conversion of
Arg to nitric oxide, and ODC1 for synthesis of polyamines
are all important for growth and development of the ovine
conceptus during pregnancy.},
Language = {eng},
Doi = {10.1095/biolreprod.110.088153},
Key = {fds204259}
}
@article{fds174155,
Author = {H Gao and G Wu and TE Spencer and GA Johnson and FW Bazer},
Title = {Select nutrients in the ovine uterine lumen. V. Nitric oxide
synthase, GTP cyclohydrolase, and ornithine decarboxylase in
ovine uteri and peri-implantation conceptuses.},
Journal = {Biology of reproduction},
Volume = {81},
Number = {1},
Pages = {67-76},
Year = {2009},
Month = {July},
ISSN = {0006-3363},
url = {http://dx.doi.org/10.1095/biolreprod.108.075473},
Keywords = {Animals • Embryo Implantation • Embryo, Mammalian
• Estrous Cycle • Female • Food* • GTP
Cyclohydrolase • Gene Expression Regulation,
Developmental • Gene Expression Regulation, Enzymologic
• Interferon Type I • Isoenzymes • Models,
Biological • Nitric Oxide Synthase • Organ
Specificity • Ornithine Decarboxylase • Pregnancy
• Pregnancy Proteins • Progesterone • Sheep
• Time Factors • Uterus • analysis •
chemistry • drug effects • embryology •
enzymology • genetics • genetics* •
metabolism • metabolism* • pharmacology •
physiology},
Abstract = {Nitric oxide (NO) and polyamines are critical for
implantation and development of conceptuses (embryo and
extraembryonic membranes), but mechanisms regulating their
biosynthesis in uteri and conceptuses are largely unknown.
This study determined the effects of the estrous cycle,
pregnancy, progesterone, and interferon tau (IFNT) on
expression of NO synthases (NOS1, NOS2, and NOS3), guanosine
triphosphate (GTP) cyclohydrolase (GCH1, the key enzyme in
de novo synthesis of tetrahydrobiopterin, a cofactor for NO
production), and ornithine decarboxylase (ODC1) in uterine
endometria in cyclic ewes (Days 10-16) and pregnant ewes
(Days 10-20). The mRNAs and proteins for NOS1 and ODC1 were
most abundant in uterine luminal (LE) and superficial
glandular (sGE) epithelia, and abundance was affected by day
of estrous cycle and early pregnancy. NOS2, GCH1, and NOS3
mRNAs were detected in very low abundance in uterine
epithelia and stromal cells in both cyclic and pregnant
ewes. NOS1 mRNA also was expressed very weakly in
conceptuses, whereas NOS3 mRNA was abundant in the
trophectoderm and endoderm of conceptuses, as were total
NOS1 and NOS3 proteins, inhibitory p-NOS1 protein, and
stimulatory p-NOS3 protein. GCH1 mRNA was abundant in the
trophectoderm and endoderm of conceptuses between Days 13
and 15 of pregnancy and then decreased thereafter, whereas
ODC1 mRNA abundance increased in conceptuses between Days 13
and 18 of pregnancy. GCH1 protein was localized primarily in
the nuclei of trophectoderm and endoderm, and its abundance
decreased after Day 14 of pregnancy, whereas ODC1 protein
was more abundant in the trophectoderm than in the endoderm
between Days 13 and 18 of pregnancy. Progesterone stimulated
NOS1 and GCH1 expression in LE/sGE and glandular epithelia,
whereas IFNT inhibited NOS1 expression in these cell types.
Thus, biosynthesis of NO and polyamines in ovine uterine
endometria and conceptuses is potentially regulated at
transcriptional, translational, and posttranslational levels
to favor conceptus development and implantation.},
Language = {eng},
Doi = {10.1095/biolreprod.108.075473},
Key = {fds174155}
}
@article{fds174196,
Author = {H Gao and G Wu and TE Spencer and GA Johnson and FW Bazer},
Title = {Select nutrients in the ovine uterine lumen. VI. Expression
of FK506-binding protein 12-rapamycin complex-associated
protein 1 (FRAP1) and regulators and effectors of mTORC1 and
mTORC2 complexes in ovine uteri and conceptuses.},
Journal = {Biology of reproduction},
Volume = {81},
Number = {1},
Pages = {87-100},
Year = {2009},
Month = {July},
ISSN = {0006-3363},
url = {http://dx.doi.org/10.1095/biolreprod.109.076257},
Keywords = {Animals • Carrier Proteins • Embryo Implantation
• Embryo, Mammalian • Estrous Cycle •
Eukaryotic Initiation Factors • Female • Food*
• Gene Expression Regulation • Gestational Age
• Interferon Type I • Models, Biological •
Organ Specificity • Pregnancy • Pregnancy Proteins
• Pregnancy, Animal • Progesterone • Protein
Kinases • Protein Multimerization • Sheep •
Transcription Factors • Uterus • drug effects
• embryology • genetics • genetics* •
metabolism • metabolism* • pharmacology •
physiology},
Abstract = {FRAP1 (FK506-binding protein 12-rapamycin complex-associated
protein 1), a component of the nutrient-sensing cell
signaling pathway, is critical for cell growth and
metabolism. The present study determined expression of FRAP1
and associated members of the mTORC1 and mTORC2 cell
signaling pathways in uteri of cyclic and pregnant ewes and
conceptuses, as well as effects of pregnancy, progesterone
(P4), and interferon tau (IFNT) on their expression. The
mRNAs for FRAP1, LST8, MAPKAP1, RAPTOR, RICTOR, TSC1, TSC2,
RHEB, and EIF4EBP1 were localized to luminal, superficial
glandular, and glandular epithelia and stromal cells of
uteri from cyclic and pregnant ewes, as well as
trophectoderm and endoderm of conceptuses between Days 13
and 18 of pregnancy. The abundance of FRAP1, RAPTOR, RICTOR,
TSC1, and TSC2 mRNAs in endometria was unaffected by
pregnancy status or by day of the estrous cycle or
pregnancy; however, levels of LST8, MAPKAP1, RHEB, and
EIF4EBP1 mRNA increased in endometria during early
pregnancy. In ovariectomized ewes, P4 and IFNT stimulated
expression of RHEB and EIF4EBP1 in uterine endometria. Total
endometrial FRAP1 protein and phosphorylated FRAP1 protein
levels were affected by pregnancy status and by day after
onset of estrus, and phosphorylated FRAP1 protein was
detected in nuclei of uterine epithelia and conceptuses. In
endometria of pregnant ewes, increases in abundance of mRNAs
for RICTOR, RHEB, and EIF4EBP1, as well as RHEB protein,
correlated with rapid conceptus growth and development
during the peri-implantation period. These results suggest
that the FRAP1 cell signaling pathway mediates interactions
between the maternal uterus and peri-implantation
conceptuses and that P4 and IFNT affect this pathway by
regulating expression of RHEB and EIF4EBP1.},
Language = {eng},
Doi = {10.1095/biolreprod.109.076257},
Key = {fds174196}
}
@article{fds180937,
Author = {JY Kim and RC Burghardt and G Wu and GA Johnson and TE Spencer and FW
Bazer},
Title = {Select Nutrients in the Ovine Uterine Lumen. VII. Effects of
Arginine, Leucine, Glutamine, and Glucose on Trophectoderm
Cell Signaling, Proliferation, and Migration.},
Journal = {Biology of reproduction},
Year = {2010},
Month = {September},
ISSN = {1529-7268},
url = {http://dx.doi.org/10.1095/biolreprod.110.085738},
Abstract = {Histotroph is required for survival and development of ovine
conceptuses (embryo and extra-embryonic membranes). Results
from our laboratory indicate that arginine (Arg), leucine
(Leu), glutamine (Gln) and glucose increase in the uterine
lumen between Days 10 and 15 of pregnancy, coincident with
increases in expression of amino acid and glucose
transporters by uterine epithelia, as well as trophectoderm
and yolk sac of conceptuses, and elongation of the conceptus
trophectoderm. Therefore, we hypothesized that Arg, Leu, Gln
and glucose have differential effects on hypertrophy,
hyperplasia and differentiated functions of trophectoderm
cells that are critical to conceptus development. Primary
ovine trophectoderm (oTr) cells isolated from Day 15
conceptuses were serum-starved for 24h in a customized
medium, deprived of select nutrients and then treated with
either Arg, Leu, Gln or glucose. Western blot analyses of
whole oTr cell extracts revealed that Arg, Leu and glucose,
but not Gln, increased phosphorylated AKT1 (pAKT1) 2.8-,
2.5-, and 1.8-fold, respectively, within 15 min and the
increase was maintained to 60 min. Arg, Leu and glucose also
stimulated 4.2-, 4.7-, and 2.3-fold increases in
phosphorylated RPS6K (pRPS6K) within 15 min, as well as
increases in pRPS6 protein between 0 and 30 min
post-treatment that were sustained to 60 min. When oTr cells
were treated with Arg, pRPS6K protein increased in nuclei,
but this was not observed in nuclei of oTr cells treated
with Leu and glucose. Immunocytochemical analyses also
revealed abundant amounts of phosphorylated RPS6 protein in
the cytoplasm of oTr cells treated with Arg, Leu and
glucose. Further, Arg and Leu increased proliferation and
migration of oTr cells. Collectively, these results indicate
that Arg, Leu and glucose, but not Gln, in histotroph
coordinately activate AKT1-MTOR and RPS6K-RPS6 cell
signaling pathways to stimulate hypertrophy, hyperplasia,
and migration of oTr cells.},
Language = {ENG},
Doi = {10.1095/biolreprod.110.085738},
Key = {fds180937}
}
@article{fds180933,
Author = {JY Kim and RC Burghardt and G Wu and GA Johnson and TE Spencer and FW
Bazer},
Title = {Select Nutrients in the Ovine Uterine Lumen. VIII. Arginine
Stimulates Proliferation of Ovine Trophectoderm Cells
Through MTOR-RPS6K-RPS6 Signaling Cascade and Synthesis of
Nitric Oxide and Polyamines.},
Journal = {Biology of reproduction},
Year = {2010},
Month = {September},
ISSN = {1529-7268},
url = {http://dx.doi.org/10.1095/biolreprod.110.085753},
Abstract = {During the peri-implantation period in sheep, L-arginine
(L-Arg) in the uterine lumen is an essential substrate for
synthesis of nitric oxide (NO) by nitric oxide synthase
(NOS) and polyamines via arginase and ornithine
decarboxylase (ODC1) required for survival and development
of ovine conceptuses (embryo and its extra-embryonic
membranes). L-Arg can stimulate hypertrophy, hyperplasia and
differentiation of the ovine conceptus trophectoderm;
however, the responsible signal transduction cascade has not
been determined. Therefore, this study examined possible
signaling pathways mediated by L-Arg, as well as effects of
two NO donors, SNAP and DETA, and putrescine (precursor for
spermidine and spermine) on oTr cell proliferation. Further,
the inhibition of these effects by L-NAME (an inhibitor of
NOS) and Nor-NOHA (an inhibitor of arginase) was assessed.
L-Arg treatment increased the abundance of phosphorylated
MTOR, RPS6K and EIF4EBP1 in oTr cells. Consistent with
activation of these cell signaling molecules, L-Arg
increased protein synthesis and reduced protein degradation
in oTr cells. Both NO and polyamines enhanced cell
proliferation in a dose-dependent manner. The effects of
L-Arg were partially inhibited by both L-NAME and Nor-NOHA.
These results indicate that L-Arg enhances production of
polyamines and NO and activates the MTOR/FRAP1-RPS6K-RPS6
signaling pathway to stimulate proliferation and migration
of oTr cells.},
Language = {ENG},
Doi = {10.1095/biolreprod.110.085753},
Key = {fds180933}
}
@article{05149022881,
Author = {Sancho, G. and Fisher, C.R. and Mills, S. and Micheli, F. and Johnson, G.A. and Lenihan, H.S. and Peterson, C.H. and Mullineaux, L.S.},
Title = {Selective predation by the zoarcid fish Thermarces cerberus
at hydrothermal vents},
Journal = {Deep-Sea Research Part I: Oceanographic Research
Papers},
Volume = {52},
Number = {5},
Pages = {837 - 844},
Year = {2005},
url = {http://dx.doi.org/10.1016/j.dsr.2004.12.002},
Keywords = {Tissue;Ecosystems;Biodiversity;Oceanography;},
Abstract = {This study investigates predation by the vent zoarcid fish
Thermarces cerberus through gastrointestinal analyses of 27
specimens collected with the submersible ALVIN at vents at
9°50 prime N on the East Pacific Rise. T. cerberus fed
most frequently on gastropod mollusks (mainly Lepetodrilus
elevatus) and amphipod crustaceans (mainly Ventiella
sulfuris). Species found occasionally in high abundance
included the swarming amphipod Halice hesmonectes and the
snail Cyathermia naticoides. Other items also found in
gastrointestinal tracts, but in very low numbers, included
polychaete worms, crustaceans and unidentified tissue
clumps. The comparison between the size distribution of L.
elevatus limpets ingested by T. cerberus and those found
attached to vestimentiferan tubes suggest that the fish may
selectively prey on large limpets. If the selective removal
of large Lepetodrilus spp. limpets by T. cerberus does
occur, then it would have potential community-level
consequences at hydrothermal vents, since these mobile
gastropods appear to inhibit the settlement of sessile vent
species, including tube-building worms. Our results suggest
possible direct and indirect effects of T. cerberus on
benthic community structure at hydrothermal vents on the
East Pacific Rise. © 2005 Elsevier Ltd. All rights
reserved.},
Key = {05149022881}
}
@article{fds268737,
Author = {Gyengesi, E and Calabrese, E and Sherrier, MC and Johnson, GA and Paxinos, G and Watson, C},
Title = {Semi-automated 3D segmentation of major tracts in the rat
brain: comparing DTI with standard histological
methods.},
Journal = {Brain Struct Funct},
Volume = {219},
Number = {2},
Pages = {539-550},
Year = {2014},
Month = {March},
ISSN = {1863-2653},
url = {http://dx.doi.org/10.1007/s00429-013-0516-8},
Abstract = {Researchers working with rodent models of neurological
disease often require an accurate map of the anatomical
organization of the white matter of the rodent brain. With
the increasing popularity of small animal MRI techniques,
including diffusion tensor imaging (DTI), there is
considerable interest in rapid segmentation methods of
neurological structures for quantitative comparisons.
DTI-derived tractography allows simple and rapid
segmentation of major white matter tracts, but the anatomic
accuracy of these computer-generated fibers is open to
question and has not been rigorously evaluated in the rat
brain. In this study, we examine the anatomic accuracy of
tractography-based segmentation in the adult rat brain. We
analysed 12 major white matter pathways using semi-automated
tractography-based segmentation alongside manual
segmentation of Gallyas silver-stained histology sections.
We applied four fiber-tracking algorithms to the DTI
data-two integration methods and two deflection methods. In
many cases, tractography-based segmentation closely matched
histology-based segmentation; however different tractography
algorithms produced dramatically different results. Results
suggest that certain white matter pathways are more amenable
to tractography-based segmentation than others. We believe
that these data will help researchers decide whether it is
appropriate to use tractography-based segmentation of white
matter structures for quantitative DTI-based analysis of
neurologic disease models.},
Doi = {10.1007/s00429-013-0516-8},
Key = {fds268737}
}
@article{fds132779,
Author = {HE Möller and XJ Chen and MS Chawla and GP Cofer and B Driehuys and LW
Hedlund, SA Suddarth and GA Johnson},
Title = {Sensitivity and resolution in 3D NMR microscopy of the lung
with hyperpolarized noble gases.},
Journal = {Magnetic resonance in medicine : official journal of the
Society of Magnetic Resonance in Medicine / Society of
Magnetic Resonance in Medicine, UNITED STATES},
Volume = {41},
Number = {4},
Pages = {800-8},
Year = {1999},
Month = {April},
ISSN = {0740-3194},
Keywords = {Animals • Guinea Pigs • Lung • Magnetic
Resonance Imaging • Microscopy • Models,
Theoretical • Noble Gases • Sensitivity and
Specificity • cytology* • diagnostic use* •
methods*},
Abstract = {Three-dimensional magnetic resonance images of the guinea
pig lung were acquired in vivo using hyperpolarized (HP)
noble gases and radial projection encoding (PE). Results
obtained with 3He (voxel size 17 microl) demonstrated high
image quality showing airway structure down to the 5th or
6th generations. Signal-to-noise ratios (SNRs) of 129Xe
images (voxel size 40 microl) were lower by about 1 order of
magnitude as a consequence of the smaller gyromagnetic
ratio, a more rapid relaxation in the gas reservoir, and
lower polarization and isotope abundance. Comparison between
experimentally obtained SNRs and results from calculations
based on a model that accounts for the three-dimensional PE
acquisition scheme and the non-equilibrium situation in HP
gas imaging yielded excellent agreement for small flip
angles. A theoretical examination of the potential
resolution in HP gas MR microscopy of the lungs suggests
that in vivo visualization of alveolar clusters distal to
respiratory bronchioles may be possible.},
Key = {fds132779}
}
@booklet{Moller99,
Author = {Möller, HE and Chen, XJ and Chawla, MS and Cofer, GP and Driehuys, B and Hedlund, LW and Suddarth, SA and Johnson, GA},
Title = {Sensitivity and resolution in 3D NMR microscopy of the lung
with hyperpolarized noble gases.},
Journal = {Magn Reson Med},
Volume = {41},
Number = {4},
Pages = {800-808},
Year = {1999},
Month = {April},
ISSN = {0740-3194},
url = {http://www.ncbi.nlm.nih.gov/pubmed/10332857},
Abstract = {Three-dimensional magnetic resonance images of the guinea
pig lung were acquired in vivo using hyperpolarized (HP)
noble gases and radial projection encoding (PE). Results
obtained with 3He (voxel size 17 microl) demonstrated high
image quality showing airway structure down to the 5th or
6th generations. Signal-to-noise ratios (SNRs) of 129Xe
images (voxel size 40 microl) were lower by about 1 order of
magnitude as a consequence of the smaller gyromagnetic
ratio, a more rapid relaxation in the gas reservoir, and
lower polarization and isotope abundance. Comparison between
experimentally obtained SNRs and results from calculations
based on a model that accounts for the three-dimensional PE
acquisition scheme and the non-equilibrium situation in HP
gas imaging yielded excellent agreement for small flip
angles. A theoretical examination of the potential
resolution in HP gas MR microscopy of the lungs suggests
that in vivo visualization of alveolar clusters distal to
respiratory bronchioles may be possible.},
Doi = {10.1002/(sici)1522-2594(199904)41:4<800::aid-mrm20>3.0.co;2-},
Key = {Moller99}
}
@booklet{Bazer01,
Author = {F. W. Bazer and T. E. Spencer and G. A. Johnson and R. C.
Burghardt and L. A. Jaeger},
Title = {Servomechanism in the ovine uterus for establishment and
maintenance of pregnancy.},
Journal = {Biology Of Reproduction},
Volume = {64},
Pages = {90 -- 90},
Year = {2001},
Key = {Bazer01}
}
@article{fds174229,
Author = {GA Johnson},
Title = {Settlement Patterns and Their Meaning.},
Journal = {Science (New York, N.Y.)},
Volume = {213},
Number = {4503},
Pages = {126-127},
Year = {1981},
Month = {July},
ISSN = {1095-9203},
url = {http://dx.doi.org/10.1126/science.213.4503.126},
Language = {ENG},
Doi = {10.1126/science.213.4503.126},
Key = {fds174229}
}
@article{fds174159,
Author = {P Riddle and GA Johnson},
Title = {Sexual harassment: what role should health educators
play?},
Journal = {Health education},
Volume = {14},
Number = {1},
Pages = {20-3},
Year = {1990},
Month = {April},
ISSN = {0097-0050},
Keywords = {Adaptation, Psychological • Employee Grievances •
Female • Health Education* • Humans • Male
• Sexual Behavior* • Social Behavior*},
Language = {eng},
Key = {fds174159}
}
@article{fds174268,
Author = {GA Johnson},
Title = {SHELLFISH CONSERVATION AND SEWAGE DISPOSAL.},
Journal = {American journal of public health (New York, N.Y. :
1912)},
Volume = {4},
Number = {11},
Pages = {965-8},
Year = {1914},
Month = {November},
ISSN = {0271-4353},
Language = {eng},
Key = {fds174268}
}
@article{8979156,
Author = {Johnson, G.A.},
Title = {Shield design for lunar surface applications},
Journal = {AIP Conf. Proc. (USA)},
Number = {813},
Pages = {701 - 6},
Year = {2006},
Keywords = {electric generators;fission reactors;lunar surface;nuclear
power;power conversion;radiation protection;},
Abstract = {A shielding concept for lunar surface applications of
nuclear power is presented herein. The reactor, primary
shield, reactor equipment and power generation module are
placed in a cavity in the lunar surface. Support structure
and heat rejection radiator panels are on the surface,
outside the cavity. The reactor power of 1,320
kW<sub>t</sub> was sized to deliver 50 kW<sub>c</sub> from a
thermoelectric power conversion subsystem. The dose rate on
the surface is less than 0.6 mRem/hr at 100 meters from the
reactor. Unoptimized shield mass is 1,020 kg which is much
lighter than a comparable π shield weighing in at 17,000
kg},
Key = {8979156}
}
@booklet{Wang01,
Author = {G. Wang and K. Pran and G. Sagvolden and G. B. Havsgard and A. E. Jensen and G. A. Johnson and S. T.
Vohra},
Title = {Ship hull structure monitoring using fibre optic
sensors},
Journal = {Smart Materials \& Structures},
Volume = {10},
Number = {3},
Pages = {472 -- 478},
Year = {2001},
Month = {June},
Key = {Wang01}
}
@booklet{Moller98,
Author = {H. E. Moller and X. J. Chen and M. S. Chawla and B. Driehuys and L. W. Hedlund and G. A. Johnson},
Title = {Signal dynamics in magnetic resonance imaging of the lung
with hyperpolarized noble gases},
Journal = {Journal Of Magnetic Resonance},
Volume = {135},
Number = {1},
Pages = {133 -- 143},
Year = {1998},
Month = {November},
Key = {Moller98}
}
@article{fds269039,
Author = {Möller, HE and Chen, XJ and Chawla, MS and Driehuys, B and Hedlund, LW and Johnson, GA},
Title = {Signal dynamics in magnetic resonance imaging of the lung
with hyperpolarized noble gases.},
Journal = {J Magn Reson},
Volume = {135},
Number = {1},
Pages = {133-143},
Year = {1998},
Month = {November},
ISSN = {1090-7807},
url = {http://www.ncbi.nlm.nih.gov/pubmed/9799687},
Keywords = {Animals • Guinea Pigs • Image Enhancement* •
Lung • Magnetic Resonance Imaging* • Magnetics
• Models, Theoretical • Noble Gases •
Reproducibility of Results • anatomy & histology*
• diagnostic use* • methods},
Abstract = {The nonequilibrium bulk magnetic moment of hyperpolarized
(HP) noble gases generated by optical pumping has unique
characteristics. Based on the Bloch equations, a model was
developed describing the signal dynamics of HP gases used in
magnetic resonance imaging (MRI) of the lung with special
consideration to the breathing cycle. Experimental
verification included extensive investigations with HP 3He
and 129Xe during both inspiration and held breath in live
guinea pigs. Radial acquisition was used to investigate the
view variations with a temporal resolution of 5 ms.
Agreement between theoretical predictions and in vivo
results was excellent. Additionally, information about
effects from noble gas diffusion and spin-lattice relaxation
was obtained. In vivo results for T1 were 28.8 +/- 1.8 s for
3He and 31.3 +/- 1.8 s for 129Xe. Comparison with in vitro
data indicated that relaxation in the pulmonary gas space is
dominated by dipolar coupling with molecular oxygen. The
results provide a quantitative basis for optimizing pulse
sequence design in HP gas MRI of the lung.},
Doi = {10.1006/jmre.1998.1563},
Key = {fds269039}
}
@booklet{Karis87,
Author = {Karis, JP and Allan Johnson and G and Glover, GH},
Title = {Signal-to-noise improvements in three-dimensional NMR
microscopy using limited-angle excitation},
Journal = {Journal of Magnetic Resonance (1969)},
Volume = {71},
Number = {1},
Pages = {24-33},
Publisher = {Elsevier BV},
Year = {1987},
Month = {January},
ISSN = {0022-2364},
url = {http://dx.doi.org/10.1016/0022-2364(87)90124-7},
Abstract = {The 3D FT variant of spin-echo imaging has previously been
successfully used to yield images at microscopic resolution.
In obtaining such high resolution, optimization of signal to
noise for a given acquisition time is crucial. Using a
limited-angle (<60) slice-selective pulse one can improve
the effective signal to noise or reduce the experimental
time. The spin echo is generated through gradient
refocusing. A phantom with T1 of 800 and 1200 ms was used to
simulate white and gray matter. Signal intensity was modeled
by the expression M= M0sinθ[1-exp(- TR T1)] 1-exp(- TR
T1)cosθ For TR < 200 ms, limited-angle excitation can yield
improvements in signal to noise by greater than a factor of
two. These results were experimentally verified on a 1.5 T
prototype system (General Electric, Milwaukee, Wis.)
configured with gradient and rf coils designed for NMR
microscopy. In those cases where signal-to-noise concerns do
not require signal averaging beyond that which is inherent
in the 3D FT technique, the limited-angle approach can
reduce the acquisition time by as much as a factor of four.
These results were verified in small animal studies of the
brain of a 200 g rat. © 1987.},
Doi = {10.1016/0022-2364(87)90124-7},
Key = {Karis87}
}
@article{fds292759,
Author = {Johnson, GA and Manson, EL and O'Foghludha, F},
Title = {Simple method of obtaining Si(Li) detector
efficiency},
Journal = {Nuclear Instruments and Methods},
Volume = {151},
Number = {1-2},
Pages = {217-220},
Publisher = {Elsevier BV},
Year = {1978},
Month = {May},
ISSN = {0029-554X},
url = {http://dx.doi.org/10.1016/0029-554X(78)90491-3},
Abstract = {The efficiency of a semi-conductor detector is measured by
compairing its response with that of an NaI(Tl) detector of
known efficiency when both are exposed to the same flux of
gamma-excited fluorescent X-rays. The experimental procedure
is outlined and corrections and limitations are discussed.
The technique is an inexpensive and convenient way of
determining semi-conductor efficiency up to about 100 keV
using a single radionuclide source of modest activity and
readily-available fluorescers. © 1978.},
Doi = {10.1016/0029-554X(78)90491-3},
Key = {fds292759}
}
@booklet{Johnson80,
Author = {Johnson, GA and O'Foghludha, F},
Title = {Simulation of mammographic x-ray spectra.},
Journal = {Med Phys},
Volume = {7},
Number = {3},
Pages = {189-195},
Year = {1980},
ISSN = {0094-2405},
url = {http://www.ncbi.nlm.nih.gov/pubmed/7393143},
Abstract = {Attempts to simulate Mo-anode spectra for film mammography
by using Mo filters with W-anode tubes have been reported by
several workers, and others have generated W-like continua
for xeromammographic purposes by heavy Al filtration of
Mo-anode tubes. In the present work the success of these
simulations was tested by Si(Li) spectrometric methods that
measured the spectral shapes and the exposure levels.
Comparisons of Mo-anode/Al-filter with W-anode/Al-filter
combinations were made, and also of W-anode/M-filter with
Mo-anode/Mo-folter combinations. In certain circumstance the
spectral shape is moderately well simulated but in all cases
the useful output is less in the simulations than in the
original spectra. The general conclusion is that simulation
is always less attractive than direct use of the desired
anode.},
Doi = {10.1118/1.594684},
Key = {Johnson80}
}
@article{fds132812,
Author = {GA Johnson and F O'Foghludha},
Title = {Simulation of mammographic x-ray spectra.},
Journal = {Medical physics, UNITED STATES},
Volume = {7},
Number = {3},
Pages = {189-95},
ISSN = {0094-2405},
Keywords = {Computers • Female • Humans • Mammography
• Spectrometry, X-Ray Emission • Xeromammography
• instrumentation • instrumentation* •
methods},
Abstract = {Attempts to simulate Mo-anode spectra for film mammography
by using Mo filters with W-anode tubes have been reported by
several workers, and others have generated W-like continua
for xeromammographic purposes by heavy Al filtration of
Mo-anode tubes. In the present work the success of these
simulations was tested by Si(Li) spectrometric methods that
measured the spectral shapes and the exposure levels.
Comparisons of Mo-anode/Al-filter with W-anode/Al-filter
combinations were made, and also of W-anode/M-filter with
Mo-anode/Mo-folter combinations. In certain circumstance the
spectral shape is moderately well simulated but in all cases
the useful output is less in the simulations than in the
original spectra. The general conclusion is that simulation
is always less attractive than direct use of the desired
anode.},
Key = {fds132812}
}
@article{fds174277,
Author = {JD Peuler and GA Johnson},
Title = {Simultaneous single isotope radioenzymatic assay of plasma
norepinephrine, epinephrine and dopamine.},
Journal = {Life sciences},
Volume = {21},
Number = {5},
Pages = {625-36},
Year = {1977},
Month = {September},
ISSN = {0024-3205},
Keywords = {Catechol O-Methyltransferase • Dopamine •
Epinephrine • Humans • Methods •
Norepinephrine • blood* • cerebrospinal fluid
• metabolism},
Language = {eng},
Key = {fds174277}
}
@booklet{Nelson99,
Author = {Nelson, RC and Johnson, GA and Spielman, AL and Lowry, CR and Sundaramoorthy, G and Sheafor, DH},
Title = {Single breath-hold dynamic subtraction CT of the liver
using-multidetector helical technology},
Journal = {Radiology},
Volume = {213P},
Pages = {125-125},
Year = {1999},
Month = {November},
Key = {Nelson99}
}
@booklet{Johnson80a,
Author = {G. A. Johnson and R. M. Kupiecki and C. A.
Baker},
Title = {Single isotope derivative (radioenzymatic) methods in the
measurement of catecholamines},
Journal = {Metabolism-clinical And Experimental},
Volume = {29},
Number = {11},
Pages = {1106 -- 1113},
Year = {1980},
Key = {Johnson80a}
}
@article{fds174243,
Author = {GA Johnson and RM Kupiecki and CA Baker},
Title = {Single isotope derivative (radioenzymatic) methods in the
measurement of catecholamines.},
Journal = {Metabolism: clinical and experimental},
Volume = {29},
Number = {11 Suppl 1},
Pages = {1106-13},
Year = {1980},
Month = {November},
ISSN = {0026-0495},
Keywords = {Catechol O-Methyltransferase* • Catecholamines •
Chemical Phenomena • Chemistry • Chromatography,
Thin Layer • Dopa Decarboxylase • Humans •
Methods • Phenylethanolamine N-Methyltransferase*
• S-Adenosylmethionine* • Tritium • analysis*
• cerebrospinal fluid},
Language = {eng},
Key = {fds174243}
}
@article{fds341778,
Author = {Anderson, RJ and Cook, JJ and Delpratt, N and Nouls, JC and Gu, B and McNamara, JO and Avants, BB and Johnson, GA and Badea,
A},
Title = {Small Animal Multivariate Brain Analysis (SAMBA) - a High
Throughput Pipeline with a Validation Framework.},
Journal = {Neuroinformatics},
Volume = {17},
Number = {3},
Pages = {451-472},
Year = {2019},
Month = {July},
url = {http://dx.doi.org/10.1007/s12021-018-9410-0},
Abstract = {While many neuroscience questions aim to understand the
human brain, much current knowledge has been gained using
animal models, which replicate genetic, structural, and
connectivity aspects of the human brain. While voxel-based
analysis (VBA) of preclinical magnetic resonance images is
widely-used, a thorough examination of the statistical
robustness, stability, and error rates is hindered by high
computational demands of processing large arrays, and the
many parameters involved therein. Thus, workflows are often
based on intuition or experience, while preclinical
validation studies remain scarce. To increase throughput and
reproducibility of quantitative small animal brain studies,
we have developed a publicly shared, high throughput VBA
pipeline in a high-performance computing environment, called
SAMBA. The increased computational efficiency allowed large
multidimensional arrays to be processed in 1-3 days-a task
that previously took ~1 month. To quantify the variability
and reliability of preclinical VBA in rodent models, we
propose a validation framework consisting of morphological
phantoms, and four metrics. This addresses several sources
that impact VBA results, including registration and template
construction strategies. We have used this framework to
inform the VBA workflow parameters in a VBA study for a
mouse model of epilepsy. We also present initial efforts
towards standardizing small animal neuroimaging data in a
similar fashion with human neuroimaging. We conclude that
verifying the accuracy of VBA merits attention, and should
be the focus of a broader effort within the community. The
proposed framework promotes consistent quality assurance of
VBA in preclinical neuroimaging, thus facilitating the
creation and communication of robust results.},
Doi = {10.1007/s12021-018-9410-0},
Key = {fds341778}
}
@article{fds132870,
Author = {HG Chotas and RL Van Metter and GA Johnson and CE
Ravin},
Title = {Small object contrast in AMBER and conventional chest
radiography.},
Journal = {Radiology, UNITED STATES},
Volume = {180},
Number = {3},
Pages = {853-9},
Year = {1991},
Month = {September},
ISSN = {0033-8419},
Keywords = {Diaphragm • Humans • Lung • Mediastinum
• Models, Structural • Radiography, Thoracic*
• Technology, Radiologic • methods •
radiography},
Abstract = {The ability of a commercially available scanning
equalization system for chest radiography to render small
object contrast in the lung-, mediastinum-, and
subdiaphragm-equivalent regions of an acrylic chest phantom
was quantitatively evaluated. Images from nine chest
phantoms that represented a wide range of patient sizes and
dynamic ranges of x-ray transmittance were analyzed. Subject
contrast was measured with a photostimulable phosphor
detector, and images were acquired in both equalized and
nonequalized (conventional) imaging modes. Available subject
contrast in the lung-equivalent region was 8%-15% lower in
the equalized images compared with the nonequalized images
in all phantoms (patient types); contrast in the
mediastinum-, retro-cardiac-, and subdiaphragm-equivalent
regions was 11%-63% higher in the equalized images, with the
degree of improvement increasing as patient size and dynamic
range increased. Images of each phantom were also acquired
with the screen-film systems currently in use at the
authors' institution, permitting an assessment of the
relative performance (in terms of radiographic contrast) of
these imagers with and without use of equalization.},
Key = {fds132870}
}
@booklet{Chotas91,
Author = {Chotas, HG and Van Metter and RL and Johnson, GA and Ravin,
CE},
Title = {Small object contrast in AMBER and conventional chest
radiography.},
Journal = {Radiology},
Volume = {180},
Number = {3},
Pages = {853-859},
Year = {1991},
Month = {September},
ISSN = {0033-8419},
url = {http://www.ncbi.nlm.nih.gov/pubmed/1871306},
Abstract = {The ability of a commercially available scanning
equalization system for chest radiography to render small
object contrast in the lung-, mediastinum-, and
subdiaphragm-equivalent regions of an acrylic chest phantom
was quantitatively evaluated. Images from nine chest
phantoms that represented a wide range of patient sizes and
dynamic ranges of x-ray transmittance were analyzed. Subject
contrast was measured with a photostimulable phosphor
detector, and images were acquired in both equalized and
nonequalized (conventional) imaging modes. Available subject
contrast in the lung-equivalent region was 8%-15% lower in
the equalized images compared with the nonequalized images
in all phantoms (patient types); contrast in the
mediastinum-, retro-cardiac-, and subdiaphragm-equivalent
regions was 11%-63% higher in the equalized images, with the
degree of improvement increasing as patient size and dynamic
range increased. Images of each phantom were also acquired
with the screen-film systems currently in use at the
authors' institution, permitting an assessment of the
relative performance (in terms of radiographic contrast) of
these imagers with and without use of equalization.},
Doi = {10.1148/radiology.180.3.1871306},
Key = {Chotas91}
}
@booklet{Maki88,
Author = {Maki, JH and Johnson, GA and Cofer, GP and MacFall,
JR},
Title = {SNR improvement in NMR microscopy using DEFT},
Journal = {Journal of Magnetic Resonance (1969)},
Volume = {80},
Number = {3},
Pages = {482-492},
Publisher = {Elsevier BV},
Year = {1988},
Month = {January},
ISSN = {0022-2364},
url = {http://dx.doi.org/10.1016/0022-2364(88)90243-0},
Abstract = {This paper examines the use of a driven equilibrium Fourier
transform (DEFT) pulse sequence for improving the signal per
unit time and hence image resolution in NMR microscopy. DEFT
vs partial saturation (PS) is modeled and it is shown that
DEFT is most useful in physiologic materials provided short
TE values (TE ≪ T2) and short TR values (TR < T1) are
used. Under these conditions, DEFT can yield up to a
fourfold signal increase compared to PS. It is shown that
DEFT can provide spin density and T1/T2-ratio-weighted
images. DEFT is also shown to have SNR advantages as T1
increases-an important consideration at higher magnetic
fields. Experimental data that verify the theoretical
predictions and the functioning of a DEFT pulse sequence to
produce high-quality 2D spin-warp images of a phantom are
presented. Studies performed on small animals demonstrate
the utility of the DEFT sequence in MR microscopy by
providing increased SNR and new contrast mechanisms over
limited fields of view. © 1988.},
Doi = {10.1016/0022-2364(88)90243-0},
Key = {Maki88}
}
@article{fds174242,
Author = {GW Fuller and GA Johnson},
Title = {SOME POINTS OF THE DIFFERENTIATION AND CLASSIFICATION OF
WATER BACTERIA.},
Journal = {Journal. Boston Society of Medical Sciences},
Volume = {4},
Number = {4},
Pages = {83-84},
Year = {1900},
Month = {January},
Language = {ENG},
Key = {fds174242}
}
@article{fds174152,
Author = {GW Fuller and GA Johnson},
Title = {Some Points on the Differentiation and Classification of
Water Bacteria.},
Journal = {Public health papers and reports},
Volume = {25},
Pages = {580-6},
Year = {1899},
ISSN = {0737-8769},
Language = {eng},
Key = {fds174152}
}
@article{fds174297,
Author = {GW Fuller and GA Johnson},
Title = {Some Points on the Differentiation and Classification of
Water Bacteria.},
Journal = {Public health papers and reports},
Volume = {25},
Pages = {580-586},
Year = {1899},
Language = {ENG},
Key = {fds174297}
}
@article{fds268865,
Author = {Song, J and Liu, QH and Johnson, GA and Badea, CT},
Title = {Sparseness prior based iterative image reconstruction for
retrospectively gated cardiac micro-CT.},
Journal = {Med Phys},
Volume = {34},
Number = {11},
Pages = {4476-4483},
Year = {2007},
Month = {November},
ISSN = {0094-2405},
url = {http://www.ncbi.nlm.nih.gov/pubmed/18072512},
Keywords = {Algorithms • Animals • Heart • Humans •
Image Processing, Computer-Assisted • Imaging,
Three-Dimensional • Least-Squares Analysis • Mice
• Mice, Inbred C57BL • Models, Statistical •
Myocardium • Phantoms, Imaging • Scattering,
Radiation • Time Factors • Tomography, X-Ray
Computed • X-Rays • metabolism • methods*
• physiology},
Abstract = {Recent advances in murine cardiac studies with
three-dimensional (3D) cone beam micro-CT used a
retrospective gating technique. However, this sampling
technique results in a limited number of projections with an
irregular angular distribution due to the temporal
resolution requirements and radiation dose restrictions.
Both angular irregularity and undersampling complicate the
reconstruction process, since they cause significant
streaking artifacts. This work provides an iterative
reconstruction solution to address this particular
challenge. A sparseness prior regularized weighted l2 norm
optimization is proposed to mitigate streaking artifacts
based on the fact that most medical images are compressible.
Total variation is implemented in this work as the
regularizer for its simplicity. Comparison studies are
conducted on a 3D cardiac mouse phantom generated with
experimental data. After optimization, the method is applied
to in vivo cardiac micro-CT data.},
Doi = {10.1118/1.2795830},
Key = {fds268865}
}
@booklet{Johnson96c,
Author = {G. A. Johnson and D. A. Mortensen and C. A.
Gotway},
Title = {Spatial and temporal analysis of weed seedling populations
using geostatistics},
Journal = {Weed Science},
Volume = {44},
Number = {3},
Pages = {704 -- 710},
Year = {1996},
Key = {Johnson96c}
}
@booklet{Colbach00,
Author = {N. Colbach and F. Forcella and G. A. Johnson},
Title = {Spatial and temporal stability of weed populations over five
years},
Journal = {Weed Science},
Volume = {48},
Number = {3},
Pages = {366 -- 377},
Year = {2000},
Key = {Colbach00}
}
@article{fds132869,
Author = {XJ Chen and HE Möller and MS Chawla and GP Cofer and B Driehuys and LW
Hedlund, GA Johnson},
Title = {Spatially resolved measurements of hyperpolarized gas
properties in the lung in vivo. Part I: diffusion
coefficient.},
Journal = {Magnetic resonance in medicine : official journal of the
Society of Magnetic Resonance in Medicine / Society of
Magnetic Resonance in Medicine, UNITED STATES},
Volume = {42},
Number = {4},
Pages = {721-8},
Year = {1999},
Month = {October},
ISSN = {0740-3194},
Keywords = {Animals • Guinea Pigs • Helium • Isotopes
• Lung • Magnetic Resonance Imaging •
Pulmonary Diffusing Capacity • Xenon Isotopes •
anatomy & histology* • diagnostic use •
methods*},
Abstract = {In imaging of hyperpolarized noble gases, a knowledge of the
diffusion coefficient (D) is important both as a contrast
mechanism and in the design of pulse sequences. We have made
diffusion coefficient maps of both hyperpolarized (3)He and
(129)Xe in guinea pig lungs. Along the length of the
trachea, (3)He D values were on average 2.4 cm(2)/sec,
closely reproducing calculated values for free gas (2.05
cm(2)/sec). The (3)He D values measured perpendicular to the
length of the trachea were approximately a factor of two
less, indicating restriction to diffusion. Further evidence
of restricted diffusion was seen in the distal pulmonary
airspaces as the average (3)He D was 0.16 cm(2)/sec. An
additional cause for the smaller (3)He D in the lung was due
to the presence of air, which is composed of heavier and
larger gases. The (129)Xe results show similar trends, with
the trachea D averaging 0.068 cm(2)/sec and the lung D
averaging 0.021 cm(2)/sec. Magn Reson Med 42:721-728,
1999.},
Key = {fds132869}
}
@booklet{Chen99,
Author = {Chen, XJ and Möller, HE and Chawla, MS and Cofer, GP and Driehuys, B and Hedlund, LW and Johnson, GA},
Title = {Spatially resolved measurements of hyperpolarized gas
properties in the lung in vivo. Part I: diffusion
coefficient.},
Journal = {Magn Reson Med},
Volume = {42},
Number = {4},
Pages = {721-728},
Year = {1999},
Month = {October},
ISSN = {0740-3194},
url = {http://www.ncbi.nlm.nih.gov/pubmed/10502761},
Abstract = {In imaging of hyperpolarized noble gases, a knowledge of the
diffusion coefficient (D) is important both as a contrast
mechanism and in the design of pulse sequences. We have made
diffusion coefficient maps of both hyperpolarized (3)He and
(129)Xe in guinea pig lungs. Along the length of the
trachea, (3)He D values were on average 2.4 cm(2)/sec,
closely reproducing calculated values for free gas (2.05
cm(2)/sec). The (3)He D values measured perpendicular to the
length of the trachea were approximately a factor of two
less, indicating restriction to diffusion. Further evidence
of restricted diffusion was seen in the distal pulmonary
airspaces as the average (3)He D was 0.16 cm(2)/sec. An
additional cause for the smaller (3)He D in the lung was due
to the presence of air, which is composed of heavier and
larger gases. The (129)Xe results show similar trends, with
the trachea D averaging 0.068 cm(2)/sec and the lung D
averaging 0.021 cm(2)/sec. Magn Reson Med 42:721-728,
1999.},
Doi = {10.1002/(sici)1522-2594(199910)42:4<721::aid-mrm14>3.0.co;2-},
Key = {Chen99}
}
@article{fds269070,
Author = {Chen, XJ and Möller, HE and Chawla, MS and Cofer, GP and Driehuys, B and Hedlund, LW and MacFall, JR and Johnson, GA},
Title = {Spatially resolved measurements of hyperpolarized gas
properties in the lung in vivo. Part II: T
*(2).},
Journal = {Magn Reson Med},
Volume = {42},
Number = {4},
Pages = {729-737},
Year = {1999},
Month = {October},
ISSN = {0740-3194},
url = {http://www.ncbi.nlm.nih.gov/pubmed/10502762},
Keywords = {Animals • Guinea Pigs • Helium • Humans
• Isotopes • Lung • Magnetic Resonance
Imaging • Male • Middle Aged • Pulmonary
Diffusing Capacity • Trachea • Xenon Isotopes
• anatomy & histology • anatomy & histology*
• diagnostic use • methods*},
Abstract = {The transverse relaxation time, T *(2), of hyperpolarized
(HP) gas in the lung in vivo is an important parameter for
pulse sequence optimization and image contrast. We obtained
T *(2) maps of HP (3)He and (129)Xe in guinea pig lungs (n =
17) and in human lungs. Eight different sets of (3)He guinea
pig studies were acquired, with variation of slice
selection, tidal volume, and oxygen level. For example, for
a (3)He tidal volume of 3 cm(3) and no slice selection, the
average T *(2) in the trachea was 14.7 ms and 8.0 ms in the
intrapulmonary airspaces. The equivalent (129)Xe experiment
yielded an average T *(2) of 40.8 ms in the trachea and 18.5
ms in the intrapulmonary airspaces. The average (3)He T *(2)
in the human intrapulmonary airspaces was 9.4 ms. The
relaxation behavior was predicted by treating the lung as a
porous medium, resulting in good agreement between estimated
and measured T *(2) values in the intrapulmonary airspaces.
Magn Reson Med 42:729-737, 1999.},
Doi = {10.1002/(sici)1522-2594(199910)42:4<729::aid-mrm15>3.0.co;2-},
Key = {fds269070}
}
@booklet{Chen99a,
Author = {X. J. Chen and H. E. Moller and M. S. Chawla and G. P. Cofer and B. Driehuys and L. W. Hedlund and J. R. Macfall and G.
A. Johnson},
Title = {Spatially resolved measurements of hyperpolarized gas
properties in the lung in vivo. Part II:
T-2*},
Journal = {Magnetic Resonance In Medicine},
Volume = {42},
Number = {4},
Pages = {729 -- 737},
Year = {1999},
Month = {October},
Key = {Chen99a}
}
@booklet{Locher96,
Author = {M. Locher and G. A. Johnson and E. R. Hunt},
Title = {Spatiotemporal stochastic resonance in a system of coupled
diode resonators},
Journal = {Physical Review Letters},
Volume = {77},
Number = {23},
Pages = {4698 -- 4701},
Year = {1996},
Month = {December},
Key = {Locher96}
}
@article{fds132911,
Author = {M Löcher and GA Johnson and ER Hunt},
Title = {Spatiotemporal Stochastic Resonance in a System of Coupled
Diode Resonators.},
Journal = {Physical review letters},
Volume = {77},
Number = {23},
Pages = {4698-4701},
Year = {1996},
Month = {December},
ISSN = {1079-7114},
Language = {ENG},
Key = {fds132911}
}
@article{fds268747,
Author = {JOHNSON, GA and OFOGHLUDHA, F},
Title = {SPECTROMETRIC STUDIES OF WAVEFORM AND FILTRATION EFFECTS IN
MAMMOGRAPHY},
Journal = {PHYSICS IN MEDICINE AND BIOLOGY},
Volume = {22},
Number = {3},
Pages = {593-593},
Year = {1977},
ISSN = {0031-9155},
url = {http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:A1977DH45600190&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=47d3190e77e5a3a53558812f597b0b92},
Key = {fds268747}
}
@booklet{Benveniste98,
Author = {Benveniste, H and Qui, H and Hedlund, LW and D'Ercole, F and Johnson,
GA},
Title = {Spinal cord neural anatomy in rats examined by in vivo
magnetic resonance microscopy.},
Journal = {Reg Anesth Pain Med},
Volume = {23},
Number = {6},
Pages = {589-599},
Year = {1998},
ISSN = {1098-7339},
url = {http://www.ncbi.nlm.nih.gov/pubmed/9840856},
Abstract = {BACKGROUND AND OBJECTIVES: Magnetic resonance microscopy
(MRM) is a technique that is worthwhile for
anesthesiologists because it allows spinal cord and plexus
anatomy to be visualized three dimensionally and followed
over time in the same animal. For example, the long-term
effect of indwelling intrathecal or plexus catheters can be
studied in situ, and convective and diffusive forces within
intrathecal, epidural, or nerve sheath spaces can be
investigated. Further, diffusion-weighted MRM, which
measures an "apparent diffusion coefficient" (ADC), can be
used to track the presence of ischemia, hypoperfusion, or
cytotoxic edema. This study investigates problems associated
with the use of in vivo MRM for spinal cord and peripheral
nerve studies in the rat. METHODS: Twenty-one anesthetized
female Fisher CDF rats were used. Group 1 (n=7) was used for
anatomic three-dimensional studies. Groups 2 (n=4), 3 (n=4),
and 4 (n=6) were used for measurements of the ADC. Group 2
served as controls, group 3 received lumbar intrathecal
catheters, and group 4 received cervical intrathecal
catheters. RESULTS: Cervical spine, lumbar spine, and spinal
nerves and ganglia were accurately visualized with MRM. As a
rule, spinal cord gray and white matter were better
demonstrated using diffusion-weighted proton stains. By
contrast, T2-weighted proton staining superiorly
demonstrated structures surrounding the spinal cord. In
groups 3 and 4, indwelling intrathecal catheters did not
affect the spinal cord ADC, indicating normal blood flow and
no cytotoxic edema. Contrast studies revealed nonhomogeneous
distribution of contrast predominately in the lateral and
ventral intrathecal space. CONCLUSION: Three-dimensional
diffusion-weighted MRM displays cervical and lumbar spine
anatomy accurately in vivo. Apparent diffusion coefficients
measurements are feasible in rat cervical spinal cord with
intrathecal catheters. Spinal cord ADCs are unaffected by
intrathecal catheters, indicating normal spinal cord
perfusion.},
Doi = {10.1016/s1098-7339(98)90087-6},
Key = {Benveniste98}
}
@article{fds132858,
Author = {H Benveniste and H Qui and LW Hedlund and F D'Ercole and GA
Johnson},
Title = {Spinal cord neural anatomy in rats examined by in vivo
magnetic resonance microscopy.},
Journal = {Regional anesthesia and pain medicine, UNITED
STATES},
Volume = {23},
Number = {6},
Pages = {589-99},
ISSN = {1098-7339},
Keywords = {Animals • Biomechanics • Catheters, Indwelling
• Cervical Vertebrae • Coloring Agents •
Contrast Media • Diffusion • Edema • Epidural
Space • Feasibility Studies • Female •
Follow-Up Studies • Injections, Spinal • Ischemia
• Longitudinal Studies • Lumbar Vertebrae •
Magnetic Resonance Imaging • Microscopy •
Neurilemma • Peripheral Nervous System Diseases •
Rats • Rats, Inbred F344 • Regional Blood Flow
• Spinal Cord • Spinal Cord Diseases • Spinal
Nerves • anatomy & histology • anatomy &
histology* • blood supply • diagnosis •
diagnostic use • instrumentation • methods* •
pathology • physiology},
Abstract = {BACKGROUND AND OBJECTIVES: Magnetic resonance microscopy
(MRM) is a technique that is worthwhile for
anesthesiologists because it allows spinal cord and plexus
anatomy to be visualized three dimensionally and followed
over time in the same animal. For example, the long-term
effect of indwelling intrathecal or plexus catheters can be
studied in situ, and convective and diffusive forces within
intrathecal, epidural, or nerve sheath spaces can be
investigated. Further, diffusion-weighted MRM, which
measures an "apparent diffusion coefficient" (ADC), can be
used to track the presence of ischemia, hypoperfusion, or
cytotoxic edema. This study investigates problems associated
with the use of in vivo MRM for spinal cord and peripheral
nerve studies in the rat. METHODS: Twenty-one anesthetized
female Fisher CDF rats were used. Group 1 (n=7) was used for
anatomic three-dimensional studies. Groups 2 (n=4), 3 (n=4),
and 4 (n=6) were used for measurements of the ADC. Group 2
served as controls, group 3 received lumbar intrathecal
catheters, and group 4 received cervical intrathecal
catheters. RESULTS: Cervical spine, lumbar spine, and spinal
nerves and ganglia were accurately visualized with MRM. As a
rule, spinal cord gray and white matter were better
demonstrated using diffusion-weighted proton stains. By
contrast, T2-weighted proton staining superiorly
demonstrated structures surrounding the spinal cord. In
groups 3 and 4, indwelling intrathecal catheters did not
affect the spinal cord ADC, indicating normal blood flow and
no cytotoxic edema. Contrast studies revealed nonhomogeneous
distribution of contrast predominately in the lateral and
ventral intrathecal space. CONCLUSION: Three-dimensional
diffusion-weighted MRM displays cervical and lumbar spine
anatomy accurately in vivo. Apparent diffusion coefficients
measurements are feasible in rat cervical spinal cord with
intrathecal catheters. Spinal cord ADCs are unaffected by
intrathecal catheters, indicating normal spinal cord
perfusion.},
Key = {fds132858}
}
@booklet{Locher97,
Author = {M. Locher and G. A. Johnson and E. R. Hunt},
Title = {Stability analysis of fixed points via chaos
control},
Journal = {Chaos},
Volume = {7},
Number = {4},
Pages = {590 -- 596},
Year = {1997},
Month = {December},
Key = {Locher97}
}
@article{fds174169,
Author = {M Locher and GA Johnson and ER Hunt},
Title = {Stability analysis of fixed points via chaos
control.},
Journal = {Chaos (Woodbury, N.Y.)},
Volume = {7},
Number = {4},
Pages = {590-596},
Year = {1997},
Month = {December},
ISSN = {1089-7682},
url = {http://dx.doi.org/10.1063/1.166258},
Abstract = {This paper reviews recent advances in the application of
chaos control techniques to the stability analysis of
two-dimensional dynamical systems. We demonstrate how the
system's response to one or multiple feedback controllers
can be utilized to calculate the characteristic multipliers
associated with an unstable periodic orbit. The experimental
results, obtained for a single and two coupled diode
resonators, agree well with the presented theory. (c) 1997
American Institute of Physics.},
Language = {ENG},
Doi = {10.1063/1.166258},
Key = {fds174169}
}
@article{fds132901,
Author = {P Parmananda and MA Rhode and GA Johnson and RW Rollins and HD Dewald and AJ Markworth},
Title = {Stabilization of unstable steady states in an
electrochemical system using derivative control.},
Journal = {Phys Rev E Stat Phys Plasmas Fluids Relat Interdiscip
Topics},
Volume = {49},
Number = {6},
Pages = {5007-5011},
Year = {1994},
Month = {June},
ISSN = {1063-651X},
Key = {fds132901}
}
@booklet{Parmananda94,
Author = {P. Parmananda and M. A. Rhode and G. A. Johnson and R. W.
Rollins and H. D. Dewald and A. J. Markworth},
Title = {Stabilization of unstable steady-states in an
electrochemical system using derivative control},
Journal = {Physical Review E},
Volume = {49},
Number = {6},
Pages = {5007 -- 5013},
Year = {1994},
Month = {June},
Key = {Parmananda94}
}
@booklet{Johnson95b,
Author = {G. A. Johnson and M. Locher and E. R. Hunt},
Title = {Stabilized spatiotemporal waves in a convectively unstable
open flow system - coupled diode resonators},
Journal = {Physical Review E},
Volume = {51},
Number = {3},
Pages = {R1625 -- R1628},
Year = {1995},
Month = {March},
Key = {Johnson95b}
}
@article{fds132912,
Author = {GA Johnson and M Löcher and ER Hunt},
Title = {Stabilized spatiotemporal waves in a convectively unstable
open flow system: coupled diode resonators.},
Journal = {Phys Rev E Stat Phys Plasmas Fluids Relat Interdiscip
Topics},
Volume = {51},
Number = {3},
Pages = {R1625-R1628},
Year = {1995},
Month = {March},
ISSN = {1063-651X},
Key = {fds132912}
}
@booklet{Locher97a,
Author = {M. Locher and G. A. Johnson and E. R. Hunt},
Title = {Stabilizing spatiotemporal patterns in a convectively
unstable open flow system via kink-antikink
pairs},
Journal = {Chaos Solitons \& Fractals},
Volume = {8},
Number = {9},
Pages = {1523 -- 1532},
Year = {1997},
Month = {September},
Key = {Locher97a}
}
@booklet{Johnson96a,
Author = {G. A. Johnson and M. Locher and E. R. Hunt},
Title = {Stable states and kink dynamics in a system of coupled diode
resonators},
Journal = {Physica D},
Volume = {96},
Number = {1-4},
Pages = {367 -- 374},
Year = {1996},
Month = {September},
Key = {Johnson96a}
}
@article{fds268852,
Author = {Petiet, A and Hedlund, L and Johnson, GA},
Title = {Staining methods for magnetic resonance microscopy of the
rat fetus.},
Journal = {J Magn Reson Imaging},
Volume = {25},
Number = {6},
Pages = {1192-1198},
Year = {2007},
Month = {June},
ISSN = {1053-1807},
url = {http://www.ncbi.nlm.nih.gov/pubmed/17520739},
Keywords = {Animals • Contrast Media • Fetus • Magnetic
Resonance Imaging • Rats • Rats, Sprague-Dawley
• Staining and Labeling • administration & dosage*
• embryology* • methods*},
Abstract = {PURPOSE: To develop a magnetic resonance histology (MRH)
staining and fixation method by immersion to enhance the
signal-to-noise ratio (SNR) with a paramagnetic contrast
agent permitting microscopic acquisition within a 3-hour
scan time. MATERIALS AND METHODS: Methods were optimized for
embryonic day 18.5 (E18.5) rat fetuses and imaging at 9.4T
with an RF refocused spin-echo pulse sequence (TR/TE = 75
msec/5.2 msec). Fixation/staining was performed by immersion
in Bouin's fixative containing varied concentrations of
ProHance (from 10:1 to 500:1 Bouin's:ProHance) and for
varied immersion durations (up to 24 hours). RESULTS: The
results showed a significant change in T1 and T2 relaxation
times as a function of concentration of contrast agent and
immersion duration. As the contrast agent penetrated the
tissues, T1 was reduced as desired (typically by 10x), but
at the same time T2 was profoundly reduced (typically by 3x)
due to both protein cross-linking from the fixative and the
high concentration of contrast agent. A systematic
assessment of this staining protocol showed an increased SNR
(by 5x) over that in unstained specimens. CONCLUSION: This
staining protocol reduced scan time for very-high-resolution
images (19.5 microm) to only 3 hours, making MRH a routine
tool for evaluating fetal development.},
Doi = {10.1002/jmri.20932},
Key = {fds268852}
}
@article{fds174126,
Author = {G Song and KA Dunlap and J Kim and DW Bailey and TE Spencer and RC
Burghardt, GF Wagner and GA Johnson and FW Bazer},
Title = {Stanniocalcin 1 is a luminal epithelial marker for
implantation in pigs regulated by progesterone and
estradiol.},
Journal = {Endocrinology},
Volume = {150},
Number = {2},
Pages = {936-45},
Year = {2009},
Month = {February},
ISSN = {1945-7170},
url = {http://dx.doi.org/10.1210/en.2008-1026},
Keywords = {Animals • Biological Markers • Embryo Implantation
• Endometrium • Epithelial Cells • Estradiol
• Female • Gene Expression Regulation •
Glycoproteins • Organ Specificity • Pregnancy
• Pregnancy, Animal* • Progesterone •
Pseudopregnancy • RNA, Messenger • Swine •
Time Factors • Uterus • drug effects • drug
effects* • genetics • genetics* • metabolism
• pharmacology* • physiology*},
Abstract = {Stanniocalcin 1 (STC1) is a glycoprotein that decreases
calcium and increases phosphate in cells/tissues. This
investigation examined endocrine regulation of STC1 in
endometria of pigs during the estrous cycle and pregnancy.
STC1 mRNA was present exclusively in luminal epithelium (LE)
between d 12 and 15 of the estrous cycle, increased between
d 12 and d 20, and was not detectable by d 30 of pregnancy.
STC1 protein was also detected in uterine flushings. To
determine effects of estrogen and progesterone, pigs were
ovariectomized and treated with these hormones alone or
together. Progesterone, but not estrogen, induced STC1 in
LE. Cotreatment with progesterone and estrogen further
stimulated STC1 over progesterone alone. To determine
effects of pseudopregnancy, nonpregnant gilts were given
daily injections of estradiol benzoate from d 11 to d 14.
STC1 was not expressed in LE on d 90 of pseudopregnancy,
suggesting that the estradiol given to induce
pseudopregnancy and/or long-term exposure to progesterone
are required for down-regulation of STC1. To determine
effects of long-term progesterone, without effects of
estradiol, pigs were ovariectomized on d 12, given daily
injections of progesterone through d 39, and hysterectomized
on d 40 after estrus. STC1 was expressed in LE of
progesterone-treated pigs, suggesting that estrogen is
involved in down-regulation of STC1. We conclude that STC1
is induced in LE by progesterone and further stimulated by
estrogen, and its down-regulation in LE by d 25 likely
requires exposure of the progestinized uterus to estrogen.
The temporal and cell type-specific expression of STC1 makes
this gene a unique marker for implantation in
pigs.},
Language = {eng},
Doi = {10.1210/en.2008-1026},
Key = {fds174126}
}
@article{fds268769,
Author = {Constantinides, C and Aristokleous, N and Johnson, GA and Perperides,
D},
Title = {Static and dynamic cardiac modelling: Initial strides and
results towards a quantitatively accurate mechanical heart
model},
Journal = {2010 7th IEEE International Symposium on Biomedical Imaging:
From Nano to Macro, ISBI 2010 - Proceedings},
Pages = {496-499},
Publisher = {IEEE},
Year = {2010},
Month = {August},
ISBN = {9781424441266},
url = {http://dx.doi.org/10.1109/ISBI.2010.5490300},
Abstract = {Magnetic Resonance Imaging (MRI) has exhibited significant
potential for quantifying cardiac function and dysfunction
in the mouse. Recent advances in highresolution cardiac MR
imaging techniques have contributed to the development of
acquisition approaches that allow fast and accurate
description of anatomic structures, and accurate surface and
finite element (FE) mesh model constructions for study of
global mechanical function in normal and transgenic mice.
This study presents work in progress for construction of
quantitatively accurate threedimensional (3D) and 4D dynamic
surface and FE models of murine left ventricular (LV) muscle
in C57BL/6J (n=10) mice. Constructed models are subsequently
imported into commercial software packages for the solution
of the constitutive equations that characterize mechanical
function, including computation of the stress and strain
fields. They are further used with solid-free form
fabrication processes to construct model-based material
renditions of the human and mouse hearts. ©2010
IEEE.},
Doi = {10.1109/ISBI.2010.5490300},
Key = {fds268769}
}
@article{fds174082,
Author = {FJ White and JW Ross and MM Joyce and RD Geisert and RC Burghardt and GA
Johnson},
Title = {Steroid regulation of cell specific secreted phosphoprotein
1 (osteopontin) expression in the pregnant porcine
uterus.},
Journal = {Biology of reproduction},
Volume = {73},
Number = {6},
Pages = {1294-301},
Year = {2005},
Month = {December},
ISSN = {0006-3363},
url = {http://dx.doi.org/10.1095/biolreprod.105.045153},
Keywords = {Animals • Corpus Luteum • Endometrium •
Epithelial Cells • Female • Fertilization •
Gene Expression Regulation, Developmental • Osteopontin
• Pregnancy • Pregnancy, Animal •
Progesterone • Pseudopregnancy • RNA, Messenger
• Sialoglycoproteins • Steroids • Sus scrofa
• Uterus • cytology • genetics* •
metabolism • metabolism* • physiology •
physiology* • secretion*},
Abstract = {Secreted phosphoprotein 1 (SPP1, commonly referred to as
osteopontin and formerly known as bone sialoprotein 1, early
T-lymphocyte activation 1) is an extracellular
matrix/adhesion molecule that is upregulated in the pregnant
uterus of all mammals examined to date. This study focused
on the pig, which has true epitheliochorial placentation and
exhibits induction of SPP1 mRNA in luminal epithelium (LE)
just before conceptus attachment and in glandular epithelium
(GE) after Day 30 of pregnancy. The objective of this study
was to determine steroid regulation of SPP1 mRNA and protein
in porcine uterine epithelium. To examine the effect of
estrogen, cyclic gilts were treated daily (Days 11-14) with
5 mg estradiol benzoate (i.m.) and hysterectomized on Day
15. To evaluate the long-term effect of pseudopregnancy,
cyclic gilts were given daily injections (Days 11-15) with
steroid as above and hysterectomized on Day 90. In situ
hybridization showed high expression of SPP1 mRNA only in LE
contiguous with apposing conceptus tissue on Day 15 of
pregnancy. In contrast, estrogen injection resulted in
moderate but uniform SPP1 mRNA in all LE of Day 15
nonpregnant gilts, with expression maintained through Day 90
of pseudopregnancy. SPP1 mRNA also localized to the GE of
Day 90 pseudopregnant gilts, similar to expression in late
gestation. Consistent with in situ hybridization results,
SPP1 protein localized to the apical surface of LE in all
estrogen-treated gilts and in the GE on Day 90 of
pseudopregnancy. We conclude that, in pregnant pigs, SPP1 is
induced by conceptus estrogen in uterine LE and is regulated
in GE in a manner coincident with CL/placental progesterone
production.},
Language = {eng},
Doi = {10.1095/biolreprod.105.045153},
Key = {fds174082}
}
@article{fds174129,
Author = {DW Harris and CA Baker and HH Saneii and GA Johnson},
Title = {Stimulation of cyclic GMP formation in smooth muscle cells
by atriopeptin II.},
Journal = {Life sciences},
Volume = {37},
Number = {7},
Pages = {591-7},
Year = {1985},
Month = {August},
ISSN = {0024-3205},
Keywords = {Animals • Atrial Natriuretic Factor • Carbachol
• Cell Line • Cyclic GMP • Diterpenes •
Female • Forskolin • Humans • Kidney •
Lung • Muscle Proteins • Muscle, Smooth, Vascular
• Nitroprusside • Pregnancy • Rabbits •
biosynthesis* • drug effects • embryology •
metabolism • metabolism* • pharmacology •
pharmacology*},
Abstract = {Addition of synthesized atriopeptin II (AP-2), a 23 amino
acid peptide of rat atria, to rat thoracic aorta smooth
muscle cells results in the stimulation of cyclic GMP
production by the cells. The EC50 for the effect is 81 nM
and a 7 fold increase occurs at 10 microM AP-2. Cyclic GMP
levels increased within 15 seconds after the addition of
AP-2 and were maximal at 5 minutes. Cyclic GMP levels in
primary rabbit kidney cells were increased 15 fold by 10
microM AP-2. However, no increase in cyclic GMP was detected
in WI-38 fibroblast cells after the addition of 10 microM
AP-2. Cyclic AMP levels were not affected by AP-2 in any of
these cell systems. The effect upon cyclic GMP accumulation
was specific for AP-2; none of the other compounds or
peptides tested affected cyclic GMP levels.},
Language = {eng},
Key = {fds174129}
}
@booklet{Harris85,
Author = {D. W. Harris and C. A. Baker and H. H. Saneii and G. A.
Johnson},
Title = {Stimulation of cyclic-gmp formation in smooth-muscle cells
by atriopeptin-ii},
Journal = {Life Sciences},
Volume = {37},
Number = {7},
Pages = {591 -- 597},
Year = {1985},
Key = {Harris85}
}
@booklet{Locher98,
Author = {M. Locher and D. Cigna and E. R. Hunt and G. A. Johnson and F. Marchesoni and L. Gammaitoni and M. E. Inchiosa and A. R.
Bulsara},
Title = {Stochastic resonance in coupled nonlinear dynamic
elements},
Journal = {Chaos},
Volume = {8},
Number = {3},
Pages = {604 -- 615},
Year = {1998},
Month = {September},
Key = {Locher98}
}
@article{fds174073,
Author = {M Locher and D Cigna and ER Hunt and GA Johnson and F Marchesoni and L
Gammaitoni, ME Inchiosa and AR Bulsara},
Title = {Stochastic resonance in coupled nonlinear dynamic
elements.},
Journal = {Chaos (Woodbury, N.Y.)},
Volume = {8},
Number = {3},
Pages = {604-615},
Year = {1998},
Month = {September},
ISSN = {1089-7682},
url = {http://dx.doi.org/10.1063/1.166342},
Abstract = {We investigate the response of a linear chain of diffusively
coupled diode resonators under the influence of thermal
noise. We also examine the connection between spatiotemporal
stochastic resonance and the presence of kink-antikink pairs
in the array. The interplay of nucleation rates and kink
speeds is briefly addressed. The experimental results are
supplemented with simulations on a coupled map lattice. We
furthermore present analytical results for the
synchronization and signal processing properties of a Phi(4)
field theory and explore the effects of various forms of
nonlinear coupling. (c) 1998 American Institute of
Physics.},
Language = {ENG},
Doi = {10.1063/1.166342},
Key = {fds174073}
}
@booklet{Posey00,
Author = {R. Posey and G. A. Johnson and S. T. Vohra},
Title = {Strain sensing based on coherent Rayleigh scattering in an
optical fibre},
Journal = {Electronics Letters},
Volume = {36},
Number = {20},
Pages = {1688 -- 1689},
Year = {2000},
Month = {September},
Key = {Posey00}
}
@article{fds363003,
Author = {Sitek, KR and Calabrese, E and Johnson, GA and Ghosh, SS and Chandrasekaran, B},
Title = {Structural Connectivity of Human Inferior Colliculus
Subdivisions Using in vivo and post mortem Diffusion MRI
Tractography.},
Journal = {Front Neurosci},
Volume = {16},
Pages = {751595},
Year = {2022},
url = {http://dx.doi.org/10.3389/fnins.2022.751595},
Abstract = {Inferior colliculus (IC) is an obligatory station along the
ascending auditory pathway that also has a high degree of
top-down convergence via efferent pathways, making it a
major computational hub. Animal models have attributed
critical roles for the IC in in mediating auditory
plasticity, egocentric selection, and noise exclusion. IC
contains multiple functionally distinct subdivisions. These
include a central nucleus that predominantly receives
ascending inputs and external and dorsal nuclei that receive
more heterogeneous inputs, including descending and
multisensory connections. Subdivisions of human IC have been
challenging to identify and quantify using standard brain
imaging techniques such as MRI, and the connectivity of each
of these subnuclei has not been identified in the human
brain. In this study, we estimated the connectivity of human
IC subdivisions with diffusion MRI (dMRI) tractography,
using both anatomical-based seed analysis as well as
unsupervised k-means clustering. We demonstrate sensitivity
of tractography to overall IC connections in both high
resolution post mortem and in vivo datasets. k-Means
clustering of the IC streamlines in both the post mortem and
in vivo datasets generally segregated streamlines based on
their terminus beyond IC, such as brainstem, thalamus, or
contralateral IC. Using fine-grained anatomical
segmentations of the major IC subdivisions, the post mortem
dataset exhibited unique connectivity patterns from each
subdivision, including commissural connections through
dorsal IC and lateral lemniscal connections to central and
external IC. The subdivisions were less distinct in the
context of in vivo connectivity, although lateral lemniscal
connections were again highest to central and external IC.
Overall, the unsupervised and anatomically driven methods
provide converging evidence for distinct connectivity
profiles for each of the IC subdivisions in both post mortem
and in vivo datasets, suggesting that dMRI tractography with
high quality data is sensitive to neural pathways involved
in auditory processing as well as top-down control of
incoming auditory information.},
Doi = {10.3389/fnins.2022.751595},
Key = {fds363003}
}
@article{fds357206,
Author = {Atik, AF and Calabrese, E and Gramer, R and Adil, SM and Rahimpour, S and Pagadala, P and Johnson, GA and Lad, SP},
Title = {Structural mapping with fiber tractography of the human
cuneate fasciculus at microscopic resolution in cervical
region.},
Journal = {Neuroimage},
Volume = {196},
Pages = {200-206},
Year = {2019},
Month = {August},
url = {http://dx.doi.org/10.1016/j.neuroimage.2019.04.030},
Abstract = {Human spinal white matter tract anatomy has been mapped
using post mortem histological information with the help of
molecular tracing studies in animal models. This study used
7 Tesla diffusion MR tractography on a human cadaver that
was harvested 24 hours post mortem to evaluate cuneate
fasciculus anatomy in cervical spinal cord. Based on this
method, for the first time much more nuanced tractographic
anatomy was used to investigate possible new routes for
cuneate fasciculus in the posterior and lateral funiculus.
Additionally, current molecular tracing studies were
reviewed, and confirmatory data was presented along with our
radiological results. Both studies confirm that upon entry
to the spinal cord, upper cervical level tracts (C1-2-3)
travel inside lateral funiculus and lower level tracts
travel medially inside the posterior funiculus after entry
at posterolateral sulcus which is different than traditional
knowledge of having cuneate fasciculus tracts concentrated
in the lateral part of posterior funiculus.},
Doi = {10.1016/j.neuroimage.2019.04.030},
Key = {fds357206}
}
@booklet{Johnson86,
Author = {JOHNSON, GA and BAILEY, JE and BRUCK, RI and MATYAC,
CA},
Title = {STUDIES OF DISEASED ROOT-TISSUE USING NUCLEAR-MAGNETIC-RESONANCE
IMAGING},
Journal = {PHYTOPATHOLOGY},
Volume = {76},
Number = {10},
Pages = {1067-1067},
Publisher = {AMER PHYTOPATHOLOGICAL SOC},
Year = {1986},
Month = {October},
ISSN = {0031-949X},
url = {http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:A1986F034600096&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=47d3190e77e5a3a53558812f597b0b92},
Key = {Johnson86}
}
@article{fds269021,
Author = {Zhou, X and Maronpot, RR and Cofer, GP and Hedlund, LW and Johnson,
GA},
Title = {Studies on bromobenzene-induced hepatotoxicity using in vivo
MR microscopy with surgically implanted RF
coils.},
Journal = {Magn Reson Med},
Volume = {31},
Number = {6},
Pages = {619-627},
Year = {1994},
Month = {June},
ISSN = {0740-3194},
url = {http://www.ncbi.nlm.nih.gov/pubmed/8057814},
Keywords = {Animals • Bromobenzenes • Electromagnetics •
Equipment Design • Female • Image Enhancement
• Liver • Magnetic Resonance Imaging •
Microscopy • Necrosis • Prostheses and Implants
• Rats • Rats, Inbred F344 • adverse effects*
• drug effects* • instrumentation • methods
• methods* • pathology},
Abstract = {Using surgically implanted RF coils at 300 MHz,
three-dimensional microscopic MR images of rat liver were
obtained in vivo to follow the development of pathology
induced by bromobenzene exposure. Formalin fixed specimens
of liver from these animals were also imaged using in vitro
MR microscopy, followed by conventional optical microscopy.
All MR images were acquired using a spin-warp pulse sequence
with TR = 950 ms and TE = 23 ms. The in vivo images were
reconstructed as 256(2) x 32 arrays with a voxel size of (50
microns)2 x 219 microns, while the in vitro images were
reconstructed as 256(2) x 128 arrays, giving an isotropic
resolution at (39 microns)3. Based on results from six
animals, we have found in all animals exposed to
bromobenzene, image intensity decreased in specific hepatic
tissue regions. These regions were well correlated to low
signal intensity areas observed in in vitro MR images at
higher resolution. Conventional optical microscopy indicated
that the low signal intensity regions corresponded to areas
of necrosis. The decrease in signal intensity is consistent
with increased local diffusion coefficients as a result of
necrosis. This study demonstrates that MR microscopy with
implanted RF coils can be successfully used to follow tissue
pathological changes in living tissues.},
Doi = {10.1002/mrm.1910310607},
Key = {fds269021}
}
@booklet{Zhou94,
Author = {X. H. Zhou and R. R. Maronpot and G. P. Cofer and L. W.
Hedlund and G. A. Johnson},
Title = {Studies on bromobenzene-induced hepatotoxicity using in-vivo
mr microscopy with surgically implanted rf
coils},
Journal = {Magnetic Resonance In Medicine},
Volume = {31},
Number = {6},
Pages = {619 -- 627},
Year = {1994},
Month = {June},
Key = {Zhou94}
}
@article{fds174136,
Author = {W Veldkamp and GA Johnson and HH Keasling},
Title = {Studies on the effects of reserpine in mice as influenced by
its diluent.},
Journal = {Journal of pharmaceutical sciences},
Volume = {57},
Number = {4},
Pages = {613-7},
Year = {1968},
Month = {April},
ISSN = {0022-3549},
Keywords = {Amines • Animals • Appetite Depressants •
Benzphetamine • Blepharoptosis • Brain •
Citrates • Dextroamphetamine • Drug Antagonism
• Hydrogen-Ion Concentration • Locomotion •
Male • Methylcellulose • Mice •
Norepinephrine • Phenethylamines • Propylamines
• Reserpine • Serotonin • Sulfates •
Suspensions • Time Factors • Vehicles* •
administration & dosage* • chemically induced •
drug effects • metabolism • pharmacology •
pharmacology*},
Language = {eng},
Key = {fds174136}
}
@article{fds132848,
Author = {GA Johnson and CA Baker},
Title = {Sulfation of minoxidil by human platelet
sulfotransferase.},
Journal = {Clinica chimica acta; international journal of clinical
chemistry, NETHERLANDS},
Volume = {169},
Number = {2-3},
Pages = {217-27},
Year = {1987},
Month = {November},
ISSN = {0009-8981},
Keywords = {Adolescent • Adult • Arylsulfotransferase •
Blood Platelets • Chromatography, High Pressure Liquid
• Humans • Hydrogen-Ion Concentration • Male
• Middle Aged • Minoxidil • Platelet Count
• Sulfurtransferases • blood* • enzymology*
• metabolism*},
Abstract = {In an attempt to determine whether (1) sulfotransferase
activity in human platelets would convert minoxidil to
minoxidil sulfate and (2) inter-subject variations in this
sulfotransferase activity could be noted, platelet
homogenates were incubated with minoxidil and 35S-PAPS in
HEPES buffer at 37 degrees C for 30 min. Radioactivity which
was extracted into ethyl acetate and shown by HPLC to elute
with authentic minoxidil sulfate was counted by
scintillation counting. Aliquots of the platelet homogenates
were also preincubated at 43 degrees C for 15 min to
determine the thermal stability of the sulfotransferase
activity. Sulfotransferase activity in platelets from 48
adult males ranged from 0.9-13.2 pmol minoxidil sulfate
produced/10(7) platelets per 30 min (mean 4.91 +/- 2.84
pmol/10(7) platelets per 30 min +/- SD). Thermal stable
sulfotransferase activity ranged from 0.2-7.6 pmol minoxidil
produced/10(7) platelets per 30 min and varied from 15 to
57% of the total sulfotransferase activity. Thus, the
results indicate that human platelets can effect the
sulfation of minoxidil and that sulfotransferase activity
does show inter-subject variation.},
Key = {fds132848}
}
@booklet{Kudlacek94,
Author = {P. E. Kudlacek and R. J. Anderson and G. A.
Johnson},
Title = {Sulfation of minoxidil by human platelet thermolabile phenol
sulfotransferase (tl pst)},
Journal = {Clinical Research},
Volume = {42},
Number = {3},
Pages = {A394 -- A394},
Year = {1994},
Month = {October},
Key = {Kudlacek94}
}
@booklet{Johnson87,
Author = {G. A. Johnson and C. A. Baker},
Title = {Sulfation of minoxidil by human-platelet
sulfotransferase},
Journal = {Clinica Chimica Acta},
Volume = {169},
Number = {2-3},
Pages = {217 -- 227},
Year = {1987},
Month = {November},
Key = {Johnson87}
}
@booklet{Baker86,
Author = {C. A. Baker and G. A. Johnson},
Title = {Sulfation of minoxidil by human-platelet
sulfotransferase},
Journal = {Federation Proceedings},
Volume = {45},
Number = {3},
Pages = {445 -- 445},
Year = {1986},
Month = {March},
Key = {Baker86}
}
@booklet{Johnson82d,
Author = {G. A. Johnson and K. J. Barsuhn and J. M.
Mccall},
Title = {Sulfation of minoxidil by liver sulfotransferase},
Journal = {Biochemical Pharmacology},
Volume = {31},
Number = {18},
Pages = {2949 -- 2954},
Year = {1982},
Key = {Johnson82d}
}
@article{fds174214,
Author = {GA Johnson and KJ Barsuhn and JM McCall},
Title = {Sulfation of minoxidil by liver sulfotransferase.},
Journal = {Biochemical pharmacology},
Volume = {31},
Number = {18},
Pages = {2949-54},
Year = {1982},
Month = {September},
ISSN = {0006-2952},
Keywords = {Animals • Biotransformation • Liver • Male
• Minoxidil • Pyrimidines • Rats • Rats,
Inbred Strains • Sulfurtransferases • enzymology*
• metabolism*},
Abstract = {The 100,000 g supernatant fraction of rat liver homogenate
contains a sulfotransferase activity which catalyzes the
sulfation of minoxidil. Synthetic minoxidil N-O sulfate and
the enzyme synthesized product had identical chromatographic
characteristics on high pressure liquid chromatography.
Minoxidil sulfate, which yields minoxidil when treated with
sulfatase, was slowly hydrolyzed in water. Several N-oxides
of other heterocycles, including several other pyrimidines,
triazines and imidazoles, were also substrates for this
sulfotransferase.},
Language = {eng},
Key = {fds174214}
}
@article{fds268846,
Author = {Rice, HE and Hsu, EW and Sheng, H and Evenson, DA and Freemerman, AJ and Safford, KM and Provenzale, JM and Warner, DS and Johnson,
GA},
Title = {Superparamagnetic iron oxide labeling and transplantation of
adipose-derived stem cells in middle cerebral artery
occlusion-injured mice.},
Journal = {AJR Am J Roentgenol},
Volume = {188},
Number = {4},
Pages = {1101-1108},
Year = {2007},
Month = {April},
ISSN = {1546-3141},
url = {http://www.ncbi.nlm.nih.gov/pubmed/17377054},
Keywords = {Adipose Tissue • Animals • Brain Ischemia •
Contrast Media* • Infarction, Middle Cerebral Artery
• Iron • Magnetic Resonance Imaging* • Mice
• Mice, Inbred C57BL • Oxides • Stem Cell
Transplantation • complications* • cytology*
• diagnosis* • diagnostic use* • etiology
• methods* • surgery*},
Abstract = {OBJECTIVE: Adipose-derived stem cells are an alternative
stem cell source for CNS therapies. The goals of the current
study were to label adipose-derived stem cells with
superparamagnetic iron oxide (SPIO) particles, to use MRI to
guide the transplantation of adipose-derived stem cells in
middle cerebral artery occlusion (MCAO)-injured mice, and to
localize donor adipose-derived stem cells in the injured
brain using MRI. We hypothesized that we would successfully
label adipose-derived stem cells and image them with MRI.
MATERIALS AND METHODS: Adipose-derived stem cells harvested
from mice inbred for green fluorescent protein were labeled
with SPIO ferumoxide particles through the use of
poly-L-lysine. Adipose-derived stem cell viability, iron
staining, and proliferation were measured after SPIO
labeling, and the sensitivity of MRI in the detection of
SPIO-labeled adipose-derived stem cells was assessed ex
vivo. Adult mice (n = 12) were subjected to unilateral MCAO.
Two weeks later, in vivo 7-T MRI was performed to guide
stereotactic transplantation of SPIO-labeled adipose-derived
stem cells into brain tissue adjacent to the infarct. After
24 hours, the mice were sacrificed for high-resolution ex
vivo 7-T or 9.4-T MRI and histologic study. RESULTS:
Adipose-derived stem cells were efficiently labeled with
SPIO particles without loss of cell viability or
proliferation. Using MRI, we guided precise transplantation
of adipose-derived stem cells. MR images of mice given
injections of SPIO-labeled adipose-derived stem cells had
hypointense regions that correlated with the histologic
findings in donor cells. CONCLUSION: MRI proved useful in
transplantation of adipose-derived stem cells in vivo. This
imaging technique may be useful for studies of CNS stem cell
therapies.},
Doi = {10.2214/AJR.06.0663},
Key = {fds268846}
}
@article{fds268922,
Author = {Banson, ML and Cofer, GP and Hedlund, LW and Johnson,
GA},
Title = {Surface coil imaging of rat spine at 7.0
T.},
Journal = {Magn Reson Imaging},
Volume = {10},
Number = {6},
Pages = {929-934},
Year = {1992},
ISSN = {0730-725X},
url = {http://www.ncbi.nlm.nih.gov/pubmed/1461090},
Keywords = {Animals • Equipment Design • Magnetic Resonance
Imaging • Rats • Rats, Sprague-Dawley • Spine
• anatomy & histology* • instrumentation},
Abstract = {An inductively coupled surface coil for imaging the rat
spine at 7 T is described. This planar circular probe was
made from microwave substrate to limit the size of the coil
and to minimize the magnetic susceptibility. The surface
coil was used as a single transmit/receive coil and as a
receive-only coil with a birdcage body coil for excitation.
The signal-to-noise ratio (SNR) of the probe was compared to
a 5-cm birdcage coil and exceeded the birdcage coil's SNR by
three to six times at superficial structures. The main
advantages of the probe are an improved SNR for superficial
structures and a simple design and use. Images with 50 x 50
x 500 micron voxels were obtained of the rat spine with
excellent anatomical detail.},
Doi = {10.1016/0730-725x(92)90447-8},
Key = {fds268922}
}
@booklet{Banson92,
Author = {M. L. Banson and G. P. Cofer and L. W. Hedlund and G. A.
Johnson},
Title = {Surface coil imaging of rat spine at 7.0-t},
Journal = {Magnetic Resonance Imaging},
Volume = {10},
Number = {6},
Pages = {929 -- 934},
Year = {1992},
Key = {Banson92}
}
@booklet{Scribner01,
Author = {D. R. Scribner and J. L. Walker and G. A. Johnson and S. D.
Mcmeekin and M. A. Gold and R. S. Mannel},
Title = {Surgical management of early-stage endometrial cancer in the
elderly: Is laparoscopy feasible?},
Journal = {Gynecologic Oncology},
Volume = {83},
Number = {3},
Pages = {563 -- 568},
Year = {2001},
Month = {December},
Key = {Scribner01}
}
@article{fds174187,
Author = {DR Scribner Jr and JL Walker and GA Johnson and SD McMeekin and MA Gold and RS Mannel},
Title = {Surgical management of early-stage endometrial cancer in the
elderly: is laparoscopy feasible?},
Journal = {Gynecologic oncology},
Volume = {83},
Number = {3},
Pages = {563-8},
Year = {2001},
Month = {December},
ISSN = {0090-8258},
url = {http://dx.doi.org/10.1006/gyno.2001.6463},
Keywords = {Aged • Aged, 80 and over • Endometrial Neoplasms
• Female • Humans • Hysterectomy, Vaginal
• Laparoscopy • Lymph Node Excision •
Neoplasm Staging • Retrospective Studies •
methods* • pathology • surgery*},
Abstract = {OBJECTIVE: To give insight into the utility of laparoscopic
staging of endometrial cancer in the elderly population by
reviewing the surgical management of clinically stage I
endometrial cancer patients. METHODS: A retrospective
analysis evaluating patients that were > or =65 years old
and had planned laparoscopic staging, traditional staging
via a laparotomy, or a transvaginal hysterectomy as
management of their early endometrial cancer. The
laparoscopic group had complete staging with bilateral
pelvic and paraaortic lymph node dissections and was
compared to the group who had staging performed via
laparotomy. Patients were identified by our institution's
database and data were collected by review of their medical
records. Data were collected on demographics, pathology, and
procedural information including completion rates, operating
room (OR) time, estimated blood loss (EBL), transfusions,
lymph node count, complications, and length of stay.
Associations between variables were analyzed by Student's t
tests and chi(2) testing using Excel v. 9.0. RESULTS: From
February 25, 1994, through December 21, 2000, 125 elderly
patients were identified. Sixty-seven patients had planned
laparoscopic staging (Group 1), 45 patients had staging via
planned laparotomy (Group 2), and 13 patients had a
transvaginal hysterectomy (Group 3). Group 1 and Group 2
were compared regarding surgical and postoperative data. Age
was not different between these groups (75.9 vs 74.7 years,
P = NS). Quetelet index was also similar (29.4 vs 29.9, P =
NS) 32.8% of Group 1 had > or =1 previous laparotomy
compared to 51.1% in Group 2 (P = NS). In Group 1, 53/67
(79.1%) had stage I or II disease compared to 29/45 (64.4%)
in Group 2 (P = NS). Laparoscopy was completed in 52/67
(77.6%) attempted procedures. The reasons for conversion to
laparotomy were obesity 7/67 (10.4%), bleeding 4/67 (6.0%),
intraperitoneal cancer 3/67 (4.5%), and adhesions 1/67
(1.5%). OR time was significantly longer in successful Group
1 patients compared to Group 2 patients (236 vs 148 min, p =
0.0001). EBL was similar between these groups (298 vs 336
ml, P = NS). Ten of 52 (19.2%) of successful Group 1
patients received a blood transfusion compared to 1/45
(2.2%) of Group 2 patients (P < 0.0001). Pelvic, common
iliac, and paraaortic lymph node counts were similar between
successful Group 1 patients and those in Group 2 combined
with those that received a laparotomy in Group 1 (17.8, 5.2,
6.6 vs 19.1, 5.1, 5.2, P = NS). Length of stay (LOS) was
significantly shorter in Group 1 versus Group 2 (3.0 vs 5.8
days, P < 0.0001). There were less fevers (6.0 vs 15.6%, P =
0.01), less postoperative ileus's (0 vs 15.6%, P < 0.001),
and less wound complications (6.0 vs 26.7%, P = 0.002) in
Group 1 compared to Group 2. Group 3 average age was 77.5
years. Concurrent medical comorbidities were the main reason
for the transvaginal approach. OR time averaged 104.5 min.
The average length of stay was 2.1 days with no procedural
or postoperative complications. CONCLUSIONS: The favorable
results from this retrospective study refute the bias that
age is a relative contraindication to laparoscopic surgery.
Laparoscopic staging was associated with an increased OR
time and an increased rate of transfusion but equivalent
blood loss and lymph node counts. Possible advantages are
decreased length of stay, less postoperative ileus, and less
infections complications. Transvaginal hysterectomy still
remains a proven option for women with serious comorbid
medical problems with short OR times, minimal complications,
and short lengths of stay.},
Language = {eng},
Doi = {10.1006/gyno.2001.6463},
Key = {fds174187}
}
@article{fds174105,
Author = {SA Kamelle and TL Rutledge and TD Tillmanns and NS Gould and DE Cohn and J
Wright, TJ Herzog and JS Rader and MA Gold and GA Johnson and JL Walker and RS Mannel and DS McMeekin},
Title = {Surgical-pathological predictors of disease-free survival
and risk groupings for IB2 cervical cancer: do the
traditional models still apply?},
Journal = {Gynecologic oncology},
Volume = {94},
Number = {2},
Pages = {249-55},
Year = {2004},
Month = {August},
ISSN = {0090-8258},
url = {http://dx.doi.org/10.1016/j.ygyno.2004.05.038},
Keywords = {Adult • Aged • Disease-Free Survival • Female
• Humans • Hysterectomy • Lymph Node Excision
• Lymphatic Metastasis • Lymphatic System •
Middle Aged • Neoplasm Staging • Retrospective
Studies • Risk Factors • Uterine Cervical
Neoplasms • methods • pathology • pathology*
• surgery*},
Abstract = {OBJECTIVE: To evaluate how the independent predictors of
recurrence for stage IB2 cervical cancers treated with
up-front radical hysterectomy apply to established risk
models. METHODS: Patients with IB2 cervical cancers
diagnosed from 1990 to 2000 were identified from tumor
registries of two institutions. Patients were classified
into risk groups: high-risk (HR) (positive nodes,
parametria, or margins), intermediate-risk (IR) (positive
lymph vascular space involvement (LVSI) with any cervical
stromal invasion (CSI), or (-) LVSI and > middle- CSI), or
low-risk (LR) (absence of HR or IR characteristics).
Disease-free survival (DFS) was estimated by Kaplan-Meier
method and comparisons between subgroups were studied by log
rank. A Cox proportional hazards model was used to determine
independent predictors of recurrence. RESULTS: We identified
86 patients with stage IB2 tumors treated by RH. We found
34% of patients to be HR, 60% IR, and 6% LR. Of the 52 IR
patients, 28 had (+) LVSI with superficial, middle, and
outer 1/3 CSI, and 24 had (-) LVSI with middle or outer 1/3
invasion. Overall, postoperative adjuvant radiation (PRT)
was used in 52% of the 86 patients, including 0/5 LR, 16/52
IR, and 29/29 HR patients. Univariate predictors of
recurrence were pelvic nodal disease, (+) LVSI, (+)
parametria, outer 1/3 CSI, and tumor size > 6 cm. Age,
grade, histology, and the use of postoperative radiation
were not associated with recurrence. Multivariate analysis
identified LVSI as the only independent predictor of
recurrence (RR 5.2, P = 0.03). Two-year DFS for LR, IR, and
HR patients was 100%, 83%, and 60%, respectively. Only 4/24
(17%) IR patients with (-) LVSI got PRT compared with 12/28
(43%) of IR patients with (+) LVSI. The 2-year DFS for IR
patients with (-) LVSI was 96%. IR (+) patients recurred
more frequently with a 2-year DFS of 71%. CONCLUSIONS:
Overall, 66% of patients with IB2 disease were classified as
having low or intermediate-risk disease. IR patients with
(-) LVSI and all LR patients did well with surgery alone.
This study defines the independent importance of LVSI and
questions the utility of published IR models when applied to
stage IB2 cervical cancer.},
Language = {eng},
Doi = {10.1016/j.ygyno.2004.05.038},
Key = {fds174105}
}
@article{fds329027,
Author = {Wei, H and Gibbs, E and Zhao, P and Wang, N and Cofer, GP and Zhang, Y and Johnson, GA and Liu, C},
Title = {Susceptibility tensor imaging and tractography of collagen
fibrils in the articular cartilage.},
Journal = {Magn Reson Med},
Volume = {78},
Number = {5},
Pages = {1683-1690},
Year = {2017},
Month = {November},
url = {http://dx.doi.org/10.1002/mrm.26882},
Abstract = {PURPOSE: To investigate the B0 orientation-dependent
magnetic susceptibility of collagen fibrils within the
articular cartilage and to determine whether susceptibility
tensor imaging (STI) can detect the 3D collagen network
within cartilage. METHODS: Multiecho gradient echo datasets
(100-μm isotropic resolution) were acquired from fixed
porcine articular cartilage specimens at 9.4 T. The
susceptibility tensor was calculated using phase images
acquired at 12 or 15 different orientations relative to B0 .
The susceptibility anisotropy of the collagen fibril was
quantified and diffusion tensor imaging (DTI) was compared
against STI. 3D tractography was performed to visualize and
track the collagen fibrils with DTI and STI. RESULTS: STI
experiments showed the distinct and significant anisotropic
magnetic susceptibility of collagen fibrils within the
articular cartilage. STI can be used to measure and quantify
susceptibility anisotropy maps. Furthermore, STI provides
orientation information of the underlying collagen network
via 3D tractography. CONCLUSION: The findings of this study
demonstrate that STI can characterize the orientation
variation of collagen fibrils where diffusion anisotropy
fails. We believe that STI could serve as a sensitive and
noninvasive marker to study the collagen fibrils
microstructure. Magn Reson Med 78:1683-1690, 2017. © 2017
International Society for Magnetic Resonance in
Medicine.},
Doi = {10.1002/mrm.26882},
Key = {fds329027}
}
@article{fds268717,
Author = {Xie, L and Dibb, R and Cofer, GP and Li, W and Nicholls, PJ and Johnson,
GA and Liu, C},
Title = {Susceptibility tensor imaging of the kidney and its
microstructural underpinnings.},
Journal = {Magn Reson Med},
Volume = {73},
Number = {3},
Pages = {1270-1281},
Year = {2015},
Month = {March},
ISSN = {0740-3194},
url = {http://dx.doi.org/10.1002/mrm.25219},
Abstract = {PURPOSE: The purpose of this study was to determine whether
susceptibility tensor imaging (STI) could overcome
limitations of current techniques to detect tubules
throughout the kidney. METHODS: Normal mouse kidneys
(n = 4) were imaged at 9.4T using a three-dimensional
gradient multi-echo sequence (55-micron isotropic
resolution). Phase images from 12 orientations were obtained
to compute the susceptibility tensor. Diffusion tensor
imaging (DTI) with 12 encoding directions was compared with
STI. Tractography was performed to visualize and track the
course of tubules with DTI and STI. Confocal microscopy was
used to identify which tubular segments of the nephron were
detected by DTI and STI. RESULTS: Diffusion anisotropy was
limited to the inner medulla of the kidney. DTI did not find
a significant number of coherent tubular tracks in the outer
medulla or cortex. With STI, we found strong susceptibility
anisotropy and many tracks in the inner and outer medulla
and in limited areas of the cortex. CONCLUSION: STI was able
to track tubules throughout the kidney, whereas DTI was
limited to the inner medulla. STI provides a novel contrast
mechanism related to local tubule microstructure and may
offer a powerful method to study the nephron.},
Doi = {10.1002/mrm.25219},
Key = {fds268717}
}
@booklet{Johnson98b,
Author = {G. A. Johnson and D. J. Mar and T. L. Carroll and L. M.
Pecora},
Title = {Synchronization and imposed bifurcations in the presence of
large parameter mismatch},
Journal = {Physical Review Letters},
Volume = {80},
Number = {18},
Pages = {3956 -- 3959},
Year = {1998},
Month = {May},
Key = {Johnson98b}
}
@booklet{Carroll98,
Author = {T. L. Carroll and G. A. Johnson},
Title = {Synchronizing broadband chaotic systems to narrow-band
signals},
Journal = {Physical Review E},
Volume = {57},
Number = {2},
Pages = {1555 -- 1558},
Year = {1998},
Month = {February},
Key = {Carroll98}
}
@booklet{Schostarez93,
Author = {H. J. Schostarez and J. M. Fisher and A. R. Diani and M. J.
Mulholland and K. L. Schull and M. J. Zaya and T. J. Vidmar and G. A. Johnson and M. F. Kubicek and S. J. Humphrey and M. P. Smith},
Title = {Synthesis and hair-growth stimulatory activity of
5-fluoro-6-(1-piperidinyl)-2,4-pyrimidinediamine, 3-oxide
(u-83,868)},
Journal = {Abstracts Of Papers Of The American Chemical
Society},
Volume = {205},
Pages = {131 -- MEDI},
Year = {1993},
Month = {March},
Key = {Schostarez93}
}
@booklet{Johnson82b,
Author = {G. A. Johnson},
Title = {Synthesized signal generator sets phase-noise
standard},
Journal = {Microwaves \& Rf},
Volume = {21},
Number = {12},
Pages = {110 -- 111},
Year = {1982},
Key = {Johnson82b}
}
@booklet{Johnson83d,
Author = {G. A. Johnson},
Title = {Synthesizer adjustments},
Journal = {Microwaves \& Rf},
Volume = {22},
Number = {1},
Pages = {15 -- 15},
Year = {1983},
Key = {Johnson83d}
}
@article{fds174303,
Author = {AJ Roberts and AP Niarchos and VA Subramanian and RM Abel and SD Herman and JE Sealey and DB Case and RP White and GA Johnson and JH Laragh and WA Gay
Jr},
Title = {Systemic hypertension associated with coronary artery bypass
surgery. Predisposing factors, hemodynamic characteristics,
humoral profile, and treatment.},
Journal = {The Journal of thoracic and cardiovascular
surgery},
Volume = {74},
Number = {6},
Pages = {846-59},
Year = {1977},
Month = {December},
ISSN = {0022-5223},
Keywords = {Adult • Aged • Blood Pressure •
Catecholamines • Coronary Artery Bypass • Coronary
Vessels • Dopamine beta-Hydroxylase • Female
• Hemodynamics • Humans • Hypertension •
Male • Middle Aged • Nitroprusside • Renin
• Vascular Resistance • adverse effects* •
blood • drug therapy • etiology* • pathology
• therapeutic use},
Abstract = {Systemic hypertension occurs in more than one third of
patients having coronary artery bypass graft (CABG)
operations. This report describes our studies in 100
consecutive patients undergoing CABG. We found that certain
preoperative clinical, angiographic, and biochemical factors
predispose to the development of perioperative hypertension.
These included a well-documented history of hypertension, an
elevated blood pressure the day prior to operation, greater
than 50 percent obstruction of the left main coronary
artery, and increased levels of dopamine beta hydroxylase
(DBH). The hemodynamic pattern of perioperative hypertension
was that of an increased systemic vascular resistance which
was associated with increased levels of plasma
catecholamines and plasma renin activity (PRA).
Nitroprusside was shown to be effective in managing CABG
hypertension, although other, more specific therapy may be
preferable.},
Language = {eng},
Key = {fds174303}
}
@article{fds132833,
Author = {RT Engelhardt and GA Johnson},
Title = {T1 rho relaxation and its application to MR
histology.},
Journal = {Magnetic resonance in medicine : official journal of the
Society of Magnetic Resonance in Medicine / Society of
Magnetic Resonance in Medicine, UNITED STATES},
Volume = {35},
Number = {5},
Pages = {781-6},
Year = {1996},
Month = {May},
ISSN = {0740-3194},
Keywords = {Animals • Embryo • Female • Histological
Techniques • Image Enhancement • Magnetic
Resonance Imaging • Magnetic Resonance Spectroscopy
• Mice • Microscopy • Phantoms, Imaging
• anatomy & histology • diagnostic use •
instrumentation • methods • methods*},
Abstract = {The application of T1 rho an an alternative contrast
parameter in high-field magnetic resonance histology (MRH)
has been investigated. Spectroscopic measurements of T1 rho
were performed on 5.75% agar and 1.0 mM MnCI2 phantoms at
9.4 T to validate the accuracy of the imaging measurements.
Image studies were performed at 2.0 and 9.4 T on
perfusion-fixed 17.5-day-old mouse embryos. T1, T2, and T1
rho relaxation times were calculated for the phantoms and
muscle, diencephalon, and liver tissues. The 5.75% agar
phantom and all tissues showed T1 rho dispersion with B1L,
whereas the 1.0 mM MnCI2 phantom showed no significant B1L
dependence. T1 rho dispersion with B(O) was observed arising
from the effects of diffusion through susceptibility-induced
gradients. T1 rho shows promise as a contrast parameter in
high-field MRH because it is capable of producing T2-like
contrast without the susceptibility artifacts associated
with T2-weighted images.},
Key = {fds132833}
}
@booklet{Engelhardt96,
Author = {Engelhardt, RT and Johnson, GA},
Title = {T1 rho relaxation and its application to MR
histology.},
Journal = {Magn Reson Med},
Volume = {35},
Number = {5},
Pages = {781-786},
Year = {1996},
Month = {May},
ISSN = {0740-3194},
url = {http://www.ncbi.nlm.nih.gov/pubmed/8722830},
Abstract = {The application of T1 rho an an alternative contrast
parameter in high-field magnetic resonance histology (MRH)
has been investigated. Spectroscopic measurements of T1 rho
were performed on 5.75% agar and 1.0 mM MnCI2 phantoms at
9.4 T to validate the accuracy of the imaging measurements.
Image studies were performed at 2.0 and 9.4 T on
perfusion-fixed 17.5-day-old mouse embryos. T1, T2, and T1
rho relaxation times were calculated for the phantoms and
muscle, diencephalon, and liver tissues. The 5.75% agar
phantom and all tissues showed T1 rho dispersion with B1L,
whereas the 1.0 mM MnCI2 phantom showed no significant B1L
dependence. T1 rho dispersion with B(O) was observed arising
from the effects of diffusion through susceptibility-induced
gradients. T1 rho shows promise as a contrast parameter in
high-field MRH because it is capable of producing T2-like
contrast without the susceptibility artifacts associated
with T2-weighted images.},
Doi = {10.1002/mrm.1910350520},
Key = {Engelhardt96}
}
@article{fds132847,
Author = {AC Nugent and GA Johnson},
Title = {T1rho imaging using magnetization-prepared projection
encoding (MaPPE).},
Journal = {Magnetic resonance in medicine : official journal of the
Society of Magnetic Resonance in Medicine / Society of
Magnetic Resonance in Medicine, UNITED STATES},
Volume = {43},
Number = {3},
Pages = {421-8},
Year = {2000},
Month = {March},
ISSN = {0740-3194},
Keywords = {Animals • Image Enhancement • Magnetic Resonance
Spectroscopy • Magnetics • Mathematics • Mice
• Phantoms, Imaging • Time Factors •
methods*},
Abstract = {T1rho contrast weighting using a magnetization-prepared
projection encoding (MaPPE) pulse sequence was investigated.
Fast radial imaging was implemented by applying
magnetization preparation pulses, each followed by multiple
RF alpha pulses encoding radial trajectories of k-space.
Acquiring multiple views per preparatory pulse imposes
view-to-view variation; the resultant distortion of the
point-spread function is examined. The issue of maximizing
signal while preserving the intended contrast weighting is
addressed. Under modification of repetition time and flip
angle (alpha), three distinct behavior regimes of the
sequence are identified. The utility of the pulse sequence
as a quantitative relaxation measurement tool is also
examined by comparing imaging and spectroscopy experiments.
A mouse was imaged in vitro to demonstrate the viability of
application to MR histology. These images exhibit the
utility of spinlocking and projection encoding as an
aftemative contrast source to both T2-weighted MaPPE images
and conventional T2-weighted spin-echo images.},
Key = {fds132847}
}
@booklet{Nugent00,
Author = {Nugent, AC and Johnson, GA},
Title = {T1rho imaging using magnetization-prepared projection
encoding (MaPPE).},
Journal = {Magn Reson Med},
Volume = {43},
Number = {3},
Pages = {421-428},
Year = {2000},
Month = {March},
ISSN = {0740-3194},
url = {http://www.ncbi.nlm.nih.gov/pubmed/10725885},
Abstract = {T1rho contrast weighting using a magnetization-prepared
projection encoding (MaPPE) pulse sequence was investigated.
Fast radial imaging was implemented by applying
magnetization preparation pulses, each followed by multiple
RF alpha pulses encoding radial trajectories of k-space.
Acquiring multiple views per preparatory pulse imposes
view-to-view variation; the resultant distortion of the
point-spread function is examined. The issue of maximizing
signal while preserving the intended contrast weighting is
addressed. Under modification of repetition time and flip
angle (alpha), three distinct behavior regimes of the
sequence are identified. The utility of the pulse sequence
as a quantitative relaxation measurement tool is also
examined by comparing imaging and spectroscopy experiments.
A mouse was imaged in vitro to demonstrate the viability of
application to MR histology. These images exhibit the
utility of spinlocking and projection encoding as an
aftemative contrast source to both T2-weighted MaPPE images
and conventional T2-weighted spin-echo images.},
Doi = {10.1002/(sici)1522-2594(200003)43:3<421::aid-mrm14>3.0.co;2-},
Key = {Nugent00}
}
@article{fds357202,
Author = {Halem, HA and Hochgeschwender, U and Rih, JK and Nelson, R and Johnson,
GA and Thiagalingam, A and Culler, MD},
Title = {TBR-760, a Dopamine-Somatostatin Compound, Arrests Growth of
Aggressive Nonfunctioning Pituitary Adenomas in
Mice.},
Journal = {Endocrinology},
Volume = {161},
Number = {8},
Year = {2020},
Month = {August},
url = {http://dx.doi.org/10.1210/endocr/bqaa101},
Abstract = {TBR-760 (formerly BIM-23A760) is a chimeric dopamine
(DA)-somatostatin (SST) compound with potent agonist
activity at both DA type 2 (D2R) and SST type 2 (SSTR2)
receptors. Studies have shown that chimeric DA-SST compounds
are more efficacious than individual DA and/or SST
analogues, either alone or combined, in inhibiting secretion
from primary cultures of human somatotroph and lactotroph
tumor cells. Nonfunctioning pituitary adenomas (NFPAs)
express both D2R and SSTR2 and, consequently, may respond to
TBR-760. We used a mouse model with the pro-opiomelanocortin
(POMC) gene knocked out that spontaneously develops
aggressive NFPAs. Genomic microarray and DA and SST receptor
messenger RNA expression analysis indicate that POMC KO
mouse tumors and human NFPAs have similar expression
profiles, despite arising from different cell lineages,
establishing POMC KO mice as a model for study of NFPAs.
Treatment with TBR-760 for 8 weeks resulted in nearly
complete inhibition of established tumor growth, whereas
tumors from vehicle-treated mice increased in size by 890 ±
0.7%. Comparing TBR-760 with its individual DA and SST
components, TBR-760 arrested tumor growth. Treatment with
equimolar or 10×-higher doses of the individual SST or DA
agonists, either alone or in combination, had no significant
effect. One exception was the lower dose of DA agonist that
induced modest suppression of tumor growth. Only the
chimeric compound TBR-760 arrested tumor growth in this
mouse model of NFPA. Further, significant tumor shrinkage
was observed in 20% of the mice treated with TBR-760. These
results support the development of TBR-760 as a therapy for
patients with NFPA.},
Doi = {10.1210/endocr/bqaa101},
Key = {fds357202}
}
@article{fds174141,
Author = {LS Hartt and SJ Carling and MM Joyce and GA Johnson and DK Vanderwall and TL Ott},
Title = {Temporal and spatial associations of oestrogen receptor
alpha and progesterone receptor in the endometrium of cyclic
and early pregnant mares.},
Journal = {Reproduction (Cambridge, England)},
Volume = {130},
Number = {2},
Pages = {241-50},
Year = {2005},
Month = {August},
ISSN = {1470-1626},
url = {http://dx.doi.org/10.1530/rep.1.00596},
Keywords = {Animals • Blotting, Northern • Endometrium •
Estrogen Receptor alpha • Estrous Cycle • Female
• Horses • Immunohistochemistry • In Situ
Hybridization • Pregnancy • Pregnancy, Animal
• RNA, Messenger • Receptors, Progesterone •
analysis • analysis* • chemistry* • genetics
• metabolism* • methods},
Abstract = {Uterine function is primarily controlled by the combined
actions of oestrogen and progesterone working through their
cognate nuclear receptors. The mechanism of establishment of
pregnancy in the mare is of interest because it involves
prolonged pre-attachment and conceptus migration phases, and
both invasive and non-invasive placental cell types, and as
such has been an important comparative model. This study
characterised regulation of oestrogen (ER) and progesterone
(PR) receptors in the endometrium of the mare during the
oestrous cycle and early pregnancy. Endometrial tissues
collected during the oestrous cycle and early pregnancy were
analysed for steady-state levels of ER and PR mRNA and
protein. Steady-state levels of ER and PR mRNA were highest
on days 0, 17 and 20 in cyclic mares and lowest on days 11
and 14. A day-by-status interaction was detected, indicating
that day 17 and day 20 pregnant mares exhibited low levels
of ER and PR compared with the corresponding days of the
oestrous cycle. In situ hybridisation analyses showed
receptor mRNA localisation primarily in the luminal
epithelium (LE), glandular epithelium (GE) and stroma around
oestrus. During dioestrus and early pregnancy, receptors
were not detected in the LE, and were lower in the stroma
and deeper GE. Changes in hybridisation intensity in these
cell types were consistent with changes in mRNA levels
detected by slot-blot hybridisation. ER and PR proteins were
detected in the nuclei of LE, GE and stromal cells.
Consistent with results from in situ hybridisation, levels
of ER and PR immunoreactivity were higher around oestrus,
declined to low levels during dioestrus and remained low
during early pregnancy. Results described here for temporal
and spatial changes in steroid receptor gene expression in
mares show the greatest similarities with those described
for cattle and sheep.},
Language = {eng},
Doi = {10.1530/rep.1.00596},
Key = {fds174141}
}
@booklet{White04,
Author = {F. J. White and M. M. Joyce and J. B. Hu and T. E. Spencer and R. C. Burghardt and G. A. Johnson},
Title = {Temporal and spatial expression of uterine osteopontin
during the murine estrous cycle and pregnancy.},
Journal = {Biology Of Reproduction},
Pages = {153 -- 154},
Year = {2004},
Key = {White04}
}
@booklet{Hartt02,
Author = {L. S. Hartt and M. M. Joyce and S. J. Sinor and H. Z. Liu and G. A. Johnson and D. K. Vanderwall and T. L.
Ott},
Title = {Temporal and spatial regulation of estrogen receptor a (ER)
and progesterone receptor (PR) expression in the endometrium
of nonpregnant and early pregnant mares.},
Journal = {Biology Of Reproduction},
Volume = {66},
Pages = {316 -- 316},
Year = {2002},
Key = {Hartt02}
}
@article{fds268790,
Author = {Johnston, SM and Johnson, GA and Badea, CT},
Title = {Temporal and spectral imaging with micro-CT.},
Journal = {Med Phys},
Volume = {39},
Number = {8},
Pages = {4943-4958},
Year = {2012},
Month = {August},
ISSN = {0094-2405},
url = {http://www.ncbi.nlm.nih.gov/pubmed/22894420},
Abstract = {PURPOSE: Micro-CT is widely used for small animal imaging in
preclinical studies of cardiopulmonary disease, but further
development is needed to improve spatial resolution,
temporal resolution, and material contrast. We present a
technique for visualizing the changing distribution of
iodine in the cardiac cycle with dual source micro-CT.
METHODS: The approach entails a retrospectively gated dual
energy scan with optimized filters and voltages, and a
series of computational operations to reconstruct the data.
Projection interpolation and five-dimensional bilateral
filtration (three spatial dimensions + time + energy) are
used to reduce noise and artifacts associated with
retrospective gating. We reconstruct separate volumes
corresponding to different cardiac phases and apply a linear
transformation to decompose these volumes into components
representing concentrations of water and iodine. Since the
resulting material images are still compromised by noise, we
improve their quality in an iterative process that minimizes
the discrepancy between the original acquired projections
and the projections predicted by the reconstructed volumes.
The values in the voxels of each of the reconstructed
volumes represent the coefficients of linear combinations of
basis functions over time and energy. We have implemented
the reconstruction algorithm on a graphics processing unit
(GPU) with CUDA. We tested the utility of the technique in
simulations and applied the technique in an in vivo scan of
a C57BL∕6 mouse injected with blood pool contrast agent at
a dose of 0.01 ml∕g body weight. Postreconstruction, at
each cardiac phase in the iodine images, we segmented the
left ventricle and computed its volume. Using the maximum
and minimum volumes in the left ventricle, we calculated the
stroke volume, the ejection fraction, and the cardiac
output. RESULTS: Our proposed method produces
five-dimensional volumetric images that distinguish
different materials at different points in time, and can be
used to segment regions containing iodinated blood and
compute measures of cardiac function. CONCLUSIONS: We
believe this combined spectral and temporal imaging
technique will be useful for future studies of
cardiopulmonary disease in small animals.},
Doi = {10.1118/1.4736809},
Key = {fds268790}
}
@booklet{Johnson94,
Author = {G. A. Johnson and D. M. Tramaglini and R. E. Levine and K.
Ohno and N. Y. Choi and S. L. Y. Woo},
Title = {Tensile and viscoelastic properties of human patellar
tendon},
Journal = {Journal Of Orthopaedic Research},
Volume = {12},
Number = {6},
Pages = {796 -- 803},
Year = {1994},
Month = {November},
Key = {Johnson94}
}
@article{fds174213,
Author = {GA Johnson and DM Tramaglini and RE Levine and K Ohno and NY Choi and SL
Woo},
Title = {Tensile and viscoelastic properties of human patellar
tendon.},
Journal = {Journal of orthopaedic research : official publication of
the Orthopaedic Research Society},
Volume = {12},
Number = {6},
Pages = {796-803},
Year = {1994},
Month = {November},
ISSN = {0736-0266},
url = {http://dx.doi.org/10.1002/jor.1100120607},
Keywords = {Adult • Aged • Aged, 80 and over • Aging
• Elasticity • Humans • Middle Aged •
Muscle Contraction* • Muscle Relaxation • Patella
• Stress, Mechanical • Tendons • Viscosity
• physiology • physiology*},
Abstract = {The tensile and viscoelastic properties of fresh-frozen,
nonirradiated human patellar tendon were investigated in two
groups of 15 specimens: one group was from individuals 29-50
years old and the other group was from individuals 64-93
years old. The central portion of each patella-patellar
tendon-tibia complex was subjected to cyclic
preconditioning, stress-relaxation, cyclic
stress-relaxation, and load to failure tests. For each age
group, stress-relaxation and stress-strain curves were
obtained, from which percentage relaxation, ultimate tensile
strength, strain at failure, modulus, and strain energy
density were determined. Viscoelastic behavior was described
with use of quasilinear viscoelasticity. The younger group
showed a 46 +/- 9% (mean +/- SD) decrease in stress after 15
minutes, whereas the older group exhibited a 50 +/- 6%
decrease. The values for ultimate tensile strength and
strain at failure, respectively, were 64.7 +/- 15.0 MPa and
14 +/- 6% for the younger group and 53.6 +/- 10.0 MPa and 15
+/- 5% for the older group. Modulus values were 660 +/- 266
MPa for the younger group and 504 +/- 222 MPa for the older
group. Except for ultimate tensile strength, which was 17%
less for the older group than for the younger one, no
statistically significant differences were found in tensile
or viscoelastic properties. This study indicated that there
were minimal differences in biomechanical properties of the
substance of the patellar tendon between younger and older
age groups.},
Language = {eng},
Doi = {10.1002/jor.1100120607},
Key = {fds174213}
}
@booklet{Godwin83,
Author = {Godwin, JD and Johnson, GA and Fram, EK},
Title = {Testing electrocardiographically-gated ct of the heart with
a motion phantom},
Journal = {Investigative Radiology},
Volume = {18},
Number = {4},
Pages = {S33-S33},
Year = {1983},
Key = {Godwin83}
}
@booklet{Kamperschroer89,
Author = {J. H. Kamperschroer and L. R. Grisham and L. E. Dudek and G.
M. Gammel and G. A. Johnson and H. W. Kugel and L. Lagin and T. E. Oconnor and P. A. Shah and P. Sichta and T. N.
Stevenson and A. Vonhalle and M. D. Williams and R.
Bastasz},
Title = {Tftr neutral beam injected power measurement},
Journal = {Review Of Scientific Instruments},
Volume = {60},
Number = {11},
Pages = {3377 -- 3385},
Year = {1989},
Month = {November},
Key = {Kamperschroer89}
}
@booklet{Ravin83,
Author = {Ravin, CE and Johnson, GA},
Title = {The 'optimal' chest radiograph},
Journal = {Seminars in Respiratory Medicine},
Volume = {5},
Number = {1},
Pages = {1-14},
Publisher = {Georg Thieme Verlag KG},
Year = {1983},
Month = {January},
url = {http://dx.doi.org/10.1055/s-2007-1011430},
Doi = {10.1055/s-2007-1011430},
Key = {Ravin83}
}
@article{fds174209,
Author = {P Luly and GA Johnson and L Bolis and BA Pethica},
Title = {The agglutination of erythrocytes by calcium phosphate
sols.},
Journal = {Biochimica et biophysica acta},
Volume = {233},
Number = {3},
Pages = {730-3},
Year = {1971},
Month = {June},
ISSN = {0006-3002},
Keywords = {Animals • Calcium Chloride • Calcium Phosphates*
• Cattle • Chemistry, Physical • Colloids
• Electrophoresis • Erythrocytes* •
Hemagglutination • Humans • Phosphates •
Physicochemical Phenomena • Rats • Salmonidae
• Sodium • Spectrophotometry},
Language = {eng},
Key = {fds174209}
}
@booklet{Macfall94,
Author = {MacFall, JS and Johnson, GA},
Title = {The architecture of plant vasculature and transport as seen
with magnetic resonance microscopy},
Journal = {Canadian Journal of Botany},
Volume = {72},
Number = {11},
Pages = {1561-1573},
Publisher = {Canadian Science Publishing},
Year = {1994},
Month = {January},
url = {http://dx.doi.org/10.1139/b94-193},
Doi = {10.1139/b94-193},
Key = {Macfall94}
}
@booklet{Gray01a,
Author = {C. A. Gray and G. A. Johnson and R. C. Burghardt and F. W.
Bazer and T. E. Spencer},
Title = {The defect in conceptus elongation in uterine gland knockout
(UGKO) ewes is due to an absence of endometrial glands, but
not differences in expression of lumenal epithelial adhesion
molecules.},
Journal = {Biology Of Reproduction},
Volume = {64},
Pages = {188 -- 188},
Year = {2001},
Key = {Gray01a}
}
@article{fds268731,
Author = {Clark, D and Johnston, SM and Johnson, GA and Badea,
CT},
Title = {The effect of scatter correction on dual energy
micro-CT},
Journal = {Progress in Biomedical Optics and Imaging - Proceedings of
SPIE},
Volume = {8668},
Publisher = {SPIE},
Year = {2013},
Month = {June},
ISBN = {9780819494429},
ISSN = {1605-7422},
url = {http://dx.doi.org/10.1117/12.2006904},
Abstract = {Dual energy (DE) CT imaging is expected to play a major role
in the diagnostic arena as it provides a quantitative
decomposition of basis materials, opening the door for new
clinical applications without significantly increasing dose
to the patient. DE-CT provides a particularly unique
opportunity in preclinical CT where new elemental contrast
agents are providing novel approaches for quantitative
tissue characterization. We have implemented DE-CT imaging
with a preclinical dual source micro-CT scanner. With this
configuration, both forward and cross-scatter can
substantially degrade image quality. This work investigated
the effect of scatter correction on the accuracy of
post-reconstruction iodine and calcium decomposition.
Scatter has been estimated using a lead beam stop technique.
Our approach involves noise reduction in the scatter
corrected images using bilateral filtering. The scatter
correction has been quantitatively evaluated using phantom
experiments and in vivo cancer imaging. As shown by our
measurements, the dual source scanning is affected more by
the cross-scatter from the high energy to the low energy
imaging chain. The scatter correction reduced the presence
of cupping artifacts and increased both the accuracy and
precision of dual energy decompositions of calcium and
iodine. On average, the root mean square errors in
retrieving true iodine and calcium concentrations via dual
energy were reduced by 32%. As a result of scatter
corrections, we expect more accurate quantification of
important vascular biomarkers such as fractional blood
volume and vascular permeability in preclinical cancer
studies. © 2013 SPIE.},
Doi = {10.1117/12.2006904},
Key = {fds268731}
}
@booklet{Johnson02f,
Author = {G. A. Johnson and L. Pipas and N. B. Newman-palmer and L. H.
Brown},
Title = {The emergency medicine rotation: A unique experience for
medical students},
Journal = {Journal Of Emergency Medicine},
Volume = {22},
Number = {3},
Pages = {307 -- 311},
Year = {2002},
Month = {April},
Key = {Johnson02f}
}
@article{fds174191,
Author = {GA Johnson and L Pipas and NB Newman-Palmer and LH
Brown},
Title = {The emergency medicine rotation: a unique experience for
medical students.},
Journal = {The Journal of emergency medicine},
Volume = {22},
Number = {3},
Pages = {307-11},
Year = {2002},
Month = {April},
ISSN = {0736-4679},
Keywords = {Clinical Clerkship • Curriculum •
Diagnosis-Related Groups • Emergency Medicine •
Emergency Service, Hospital • Hospitals, Teaching
• Humans • Internal Medicine • New York
• Program Evaluation • Prospective Studies •
education • education* • methods* •
statistics & numerical data},
Abstract = {The objective of this study was to determine if an Emergency
Medicine (EM) rotation for medical students offers a unique
educational opportunity, and to document those experiences.
Thirty-three medical students at one teaching hospital
recorded in a computer database information about their
patient encounters during EM and Internal Medicine (IM)
rotations. Data collected included the types of patients
seen, the level of participation in patient care and
decision making, and procedures performed. A total of 2740
patient encounters were recorded, 1564 EM and 1176 IM.
Students on EM rotations were more likely than students on
IM rotations to be involved in the initial evaluation (93.1%
vs. 47.0%, respectively), diagnosis (93.5% vs. 44.7%,
respectively), and decision making (93.3% vs. 43.5%,
respectively); they were also more likely to perform
procedures (31.7% vs. 8.5%, respectively). There were
significant differences in the patient populations and
disease processes encountered on the two rotations as
well.},
Language = {eng},
Key = {fds174191}
}
@booklet{Nielsen94,
Author = {C. H. Nielsen and G. A. Johnson},
Title = {The experience of complete neuromuscular blockade in awake
patients},
Journal = {Journal Of Clinical Anesthesia},
Volume = {6},
Number = {5},
Pages = {450 -- 450},
Year = {1994},
Key = {Nielsen94}
}
@article{fds174162,
Author = {CH Nielsen and GA Johnson},
Title = {The experience of complete neuromuscular blockade in awake
patients.},
Journal = {Journal of clinical anesthesia},
Volume = {6},
Number = {5},
Pages = {450},
Year = {1995},
Month = {January},
ISSN = {0952-8180},
Keywords = {Awareness* • Humans • Ketamine • Lung •
Neuromuscular Blocking Agents • Oxygen •
Positive-Pressure Respiration • Pulmonary Atelectasis
• Tidal Volume • administration & dosage •
administration & dosage* • blood* • chemically
induced • drug effects • pharmacology •
physiopathology},
Language = {eng},
Key = {fds174162}
}
@booklet{Dockery88,
Author = {DOCKERY, S and SUDDARTH, SA and COFER, GP and JOHNSON,
GA},
Title = {THE IMPACT OF INCREASED SPIN-LATTICE RELAXATION-TIME
MICROSCOPY AT 7.0T},
Journal = {INVESTIGATIVE RADIOLOGY},
Volume = {23},
Number = {9},
Pages = {S20-S20},
Publisher = {LIPPINCOTT-RAVEN PUBL},
Year = {1988},
Month = {September},
ISSN = {0020-9996},
url = {http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:A1988Q574500089&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=47d3190e77e5a3a53558812f597b0b92},
Key = {Dockery88}
}
@article{fds174160,
Author = {A Banerjee and R Rose and GA Johnson and RC Burghardt and SK
Ramaiah},
Title = {The influence of estrogen on hepatobiliary osteopontin
(SPP1) expression in a female rodent model of alcoholic
steatohepatitis.},
Journal = {Toxicologic pathology},
Volume = {37},
Number = {4},
Pages = {492-501},
Year = {2009},
ISSN = {1533-1601},
url = {http://dx.doi.org/10.1177/0192623309335633},
Keywords = {Alanine Transaminase • Analysis of Variance •
Animals • Disease Models, Animal • Dose-Response
Relationship, Drug • Estradiol • Estrous Cycle
• Ethanol • Fatty Liver, Alcoholic • Female
• Gene Expression • In Situ Hybridization •
Liver • Neutrophil Infiltration • Osteopontin
• Ovariectomy • Rats • Rats, Sprague-Dawley
• administration & dosage • drug effects •
genetics • immunology • metabolism •
metabolism* • pathology • pharmacology •
pharmacology*},
Abstract = {Our recent studies suggest that higher neutrophil
infiltration in females correlates with increased
hepatobiliary expression of osteopontin (OPN) in alcoholic
steatohepatitis (ASH). The objective of this study was to
understand the role of alcohol in altering estrogen levels
in females by examining the effect of ethanol (EtOH) on the
estrous cycle and then investigate the potential
relationship between estradiol (E2) and hepatobiliary OPN
expression in a female rat ASH model. Ovariectomized (OVX)
and E2-implanted OVX rats in the ASH group were evaluated
for OPN mRNA and protein expression. Low doses of E2
resulted in significant down-regulation of OPN protein and
mRNA as compared to the OVX group. However, with increasing
doses of E2, there was up-regulation of both OPN mRNA and
protein. Osteopontin was localized primarily to the biliary
epithelium. Liver injury assessed by serum ALT and
histopathology revealed a pattern similar to OPN expression.
In all groups, hepatic neutrophilic infiltration correlated
positively with OPN expression. Based on these data, we
conclude that in our ASH model, low doses of E2 appear to be
hepatoprotective, whereas the protective effect appears to
diminish with increasing doses of E2, although additional
cause and effect studies are needed for confirmation.},
Language = {eng},
Doi = {10.1177/0192623309335633},
Key = {fds174160}
}
@booklet{Johnson02c,
Author = {G. A. Johnson and M. M. Joyce and S. J. Yankey and T. R.
Hansen and T. L. Ott},
Title = {The interferon stimulated genes (ISG) 17 and Mx have
different temporal and spatial expression in the ovine
uterus suggesting more complex regulation of the Mx
gene},
Journal = {Journal Of Endocrinology},
Volume = {174},
Number = {2},
Year = {2002},
Month = {August},
Key = {Johnson02c}
}
@article{fds174163,
Author = {GA Johnson and MM Joyce and SJ Yankey and TR Hansen and TL
Ott},
Title = {The Interferon Stimulated Genes (ISG) 17 and Mx have
different temporal and spatial expression in the ovine
uterus suggesting more complex regulation of the Mx
gene.},
Journal = {The Journal of endocrinology},
Volume = {174},
Number = {2},
Pages = {R7-R11},
Year = {2002},
Month = {August},
ISSN = {0022-0795},
Keywords = {Animals • Estrous Cycle • Female •
GTP-Binding Proteins* • Gene Expression Regulation
• Gestational Age • Immunohistochemistry • In
Situ Hybridization • Pregnancy • Pregnancy
Proteins • Pregnancy, Animal • Proteins •
RNA, Messenger • Random Allocation • Sheep •
Uterus • analysis* • chemistry* • genetics*
• metabolism* • methods},
Abstract = {Interferon stimulated gene 17 (ISG17) and Mx are
up-regulated in the ruminant uterus in response to
interferon-tau (IFNtau) during early pregnancy. Recent
evidence strongly indicates that expression of ISGs occur
only in stroma (ST) and glandular epithelium (GE) during
this time as a result of transcriptional repression by
interferon regulatory factor two (IRF-2) expression in the
LE. The present report tested this hypothesis by examining
mRNA and protein expression of ISG17 and Mx in serial
uterine cross-sections obtained from cyclic and early
pregnant ewes. In situ and immunocytochemical analysis
revealed that ISG17 mRNA and protein were low to
undetectable, whereas Mx mRNA was expressed in the lumenal
(LE) and superficial GE at all days of the estrous cycle
examined. Both ISG17 and Mx mRNA increased in the stratum
compactum ST between Days 11 and 13, and expression extended
into the deep GE and stratum spongiosum ST on Days 15
through 17 in pregnant ewes. Interestingly the Mx gene
continued to be strongly expressed in LE and superficial GE
through Day 17 of pregnancy, whereas ISG17 remained low to
undetectable in these cells. Collectively, this study
highlights the complexity of the uterine environment by
unequivocally illustrating differential temporal and spatial
expression of the IFN-responsive genes ISG17 and
Mx.},
Language = {eng},
Key = {fds174163}
}
@article{fds174232,
Author = {GA Johnson},
Title = {The interview workshop.},
Journal = {Journal of medical education},
Volume = {49},
Number = {1},
Pages = {75-7},
Year = {1974},
Month = {January},
ISSN = {0022-2577},
Keywords = {Education, Continuing* • Family Practice • Female
• Humans • Interview, Psychological* • Male
• Michigan • Minority Groups • Primary Health
Care • Rural Population • Schools, Medical* •
Social Problems • Students, Medical • Videotape
Recording},
Language = {eng},
Key = {fds174232}
}
@article{fds174272,
Author = {GA Johnson and KG Alberti},
Title = {The metabolic effects of sodium dichloroacetate in
experimental hepatitis in the rat [proceedings]},
Journal = {Biochemical Society transactions},
Volume = {5},
Number = {5},
Pages = {1387-9},
Year = {1977},
ISSN = {0300-5127},
Keywords = {Acetic Acids • Animals • Blood Glucose •
Dichloroacetate • Fructose • Galactosamine •
Hepatitis, Animal • Ketone Bodies • Lactates
• Rats • blood • blood* • metabolism
• pharmacology • pharmacology*},
Language = {eng},
Key = {fds174272}
}
@booklet{Cardina97,
Author = {J. Cardina and G. A. Johnson and D. H. Sparrow},
Title = {The nature and consequence of weed spatial
distribution},
Journal = {Weed Science},
Volume = {45},
Number = {3},
Pages = {364 -- 373},
Year = {1997},
Key = {Cardina97}
}
@booklet{Diani91,
Author = {A. R. Diani and D. J. Waldon and S. J. Conrad and M. J.
Mulholland and K. L. Shull and M. F. Kubicek and G. A.
Johnson and M. N. Brunden and A. E. Buhl},
Title = {The opening of potassium channels - a mechanism for
hair-growth},
Journal = {Annals Of The New York Academy Of Sciences},
Volume = {642},
Pages = {504 -- 504},
Year = {1991},
Month = {December},
Key = {Diani91}
}
@article{fds174199,
Author = {GA Johnson and DW Baldridge},
Title = {The orthodontic gospel according to Begg.},
Journal = {Journal of the Louisiana Dental Association},
Volume = {24},
Number = {1},
Pages = {5-8},
Year = {1966},
ISSN = {0024-6786},
Keywords = {Dentistry • Orthodontics, Corrective},
Language = {eng},
Key = {fds174199}
}
@booklet{Burvill96,
Author = {P. W. Burvill and G. A. Johnson and T. M. H. Chakera and E.
G. Stewartwynne and C. S. Anderson and K. D.
Jamrozik},
Title = {The place of site of lesion in the aetiology of post-stroke
depression},
Journal = {Cerebrovascular Diseases},
Volume = {6},
Number = {4},
Pages = {208 -- 215},
Year = {1996},
Key = {Burvill96}
}
@article{fds174069,
Author = {GA Johnson and WR Copeland and AE Kimberly},
Title = {The Relative Applicability of Current Methods for the
Determination of Putrescibility in Sewage
Effluents.},
Journal = {Public health papers and reports},
Volume = {31},
Number = {Pt 2},
Pages = {80-96},
Year = {1905},
Language = {ENG},
Key = {fds174069}
}
@article{fds174197,
Author = {GA Johnson and WR Copeland and AE Kimberly},
Title = {The Relative Applicability of Current Methods for the
Determination of Putrescibility in Sewage
Effluents.},
Journal = {Public health papers and reports},
Volume = {31},
Number = {Pt 2},
Pages = {80-96},
Year = {1905},
ISSN = {0737-8769},
Language = {eng},
Key = {fds174197}
}
@article{fds174154,
Author = {HE Williams and GA Johnson and LH Smith Jr},
Title = {The renal clearance of oxalate in normal subjects and
patients with primary hyperoxaluria.},
Journal = {Clinical science},
Volume = {41},
Number = {3},
Pages = {213-8},
Year = {1971},
Month = {September},
ISSN = {0009-9287},
Keywords = {Adult • Carbon Isotopes • Creatinine • Humans
• Kidney • Male • Metabolism, Inborn Errors
• Oxalates • blood • metabolism •
metabolism* • urine*},
Language = {eng},
Key = {fds174154}
}
@article{fds174156,
Author = {KA Dunlap and HI Kwak and RC Burghardt and FW Bazer and RR Magness and GA
Johnson, KJ Bayless},
Title = {The sphingosine 1-phosphate (S1P) signaling pathway is
regulated during pregnancy in sheep.},
Journal = {Biology of reproduction},
Volume = {82},
Number = {5},
Pages = {876-87},
Year = {2010},
Month = {May},
ISSN = {1529-7268},
url = {http://dx.doi.org/10.1095/biolreprod.109.081604},
Abstract = {Because sphingosine 1-phosphate (S1P) is a potent stimulator
of angiogenesis, we hypothesized that the S1P pathway is
activated to stimulate endometrial/placental angiogenesis
during pregnancy. We initially localized S1P signaling
pathway members in the gravid and nongravid uterine horns of
unilaterally pregnant ewes. Sphingosine kinase-1 expression
was greater in gravid compared to nongravid horns. In situ
hybridization revealed elevated expression of sphingosine
1-phosphate phosphatase (SGPP1) in gravid interplacentomal
endometrial stroma on Days 20 and 40 compared to the
nongravid uterine horn, but expression increased in
endometrium of the nongravid uterine horn between Days 40
and 120. SGPP1 expression increased in placentomes late in
gestation. Sphingosine 1-phosphate lyase mRNA was modestly
expressed at Day 20 and then decreased. In contrast,
sphingosine 1-phosphate receptor 1 (S1PR1) mRNA increased in
endometrium and caruncular stroma of the gravid uterine
horn. Treatment with FTY720 and VPC23019, S1P receptor
antagonists, blocked human and ovine endothelial cell
invasion using an in vitro model of sprouting angiogenesis.
Knockdown of S1PR1 with siRNA reduced invasion responses as
well. We previously reported that delta-like 4 (DLL4) and A
disintegrin and metalloproteinase with thrombospondin-like
repeats 1 (ADAMTS1) participate in endothelial cell invasion
stimulated by S1P and growth factors in vitro, and thus
investigated whether their expression correlated with areas
undergoing angiogenesis in vivo. DLL4 expression was similar
to S1PR1, while ADAMTS1 mRNA was expressed by endometria of
both nongravid and gravid horns, as well as conceptus and
placentomes. These results establish that S1P signaling
pathway members and S1P- and growth factor-regulated genes
are prominent in uterine and placental tissue and in some
cases are correlated with areas undergoing angiogenesis.
Thus, S1P signaling may be crucial for proper
fetal-placental development.},
Language = {eng},
Doi = {10.1095/biolreprod.109.081604},
Key = {fds174156}
}
@booklet{Johnson95,
Author = {G. A. Johnson and D. A. Mortensen and L. J. Young and A. R.
Martin},
Title = {The stability of weed seedling population-models and
parameters in eastern nebraska corn (zea-mays) and soybean
(glycine-max) fields},
Journal = {Weed Science},
Volume = {43},
Number = {4},
Pages = {604 -- 611},
Year = {1995},
Key = {Johnson95}
}
@article{fds174251,
Author = {A Banerjee and RC Burghardt and GA Johnson and FJ White and SK
Ramaiah},
Title = {The temporal expression of osteopontin (SPP-1) in the rodent
model of alcoholic steatohepatitis: a potential
biomarker.},
Journal = {Toxicologic pathology},
Volume = {34},
Number = {4},
Pages = {373-84},
Year = {2006},
ISSN = {0192-6233},
url = {http://dx.doi.org/10.1080/01926230600806543},
Keywords = {Alanine Transaminase • Animals • Aspartate
Aminotransferases • Biological Markers • Disease
Models, Animal • Fatty Liver, Alcoholic • Gene
Expression • Immunohistochemistry • In Situ
Hybridization • Inflammation • Lipopolysaccharides
• Liver Function Tests • Male • Neutrophil
Infiltration • Osteopontin • RNA, Messenger •
Rats • Rats, Sprague-Dawley • Sialoglycoproteins
• Time Factors • blood • chemically induced
• drug effects • etiology • genetics •
metabolism • metabolism* • pathology •
pathology* • pharmacology},
Abstract = {Previous studies from our laboratory have shown that
osteopontin (OPN) mediates neutrophil infiltration into the
liver in a rodent model of alcoholic steatohepatitis (ASH).
The objective of this study was to investigate the temporal
and spatial pattern of hepatic OPN mRNA and protein
expression during the progression of alcoholic liver
disease. OPN mRNA and protein expression were evaluated
using real time PCR, in situ hybridization, Western blot and
immunohistochemistry respectively. ASH was induced in male
Sprague-Dawley rats by feeding EtOH-containing
Lieber-DeCarli diet for 6 weeks, followed by a single
injection of lipopolysaccharide (LPS, 10 mg/kg, ip). Rats
were sacrificed 2-, 12-and 24-hour post LPS injection. A
progressive induction of OPN mRNA was observed that preceded
hepatic neutrophil infiltration and the increase in OPN mRNA
correlated with increases in OPN protein expression. OPN
mRNA was localized primarily to the biliary epithelium. The
data indicates that OPN is transcribed and translated within
the biliary epithelium. These findings suggest a potential
role of OPN as an early biomarker in predicting inflammatory
liver diseases such as ASH.},
Language = {eng},
Doi = {10.1080/01926230600806543},
Key = {fds174251}
}
@article{fds174099,
Author = {AH Fishel and GA Johnson},
Title = {The three-way conference -- nursing student, nursing
supervisor and nursing educator.},
Journal = {The Journal of nursing education},
Volume = {20},
Number = {6},
Pages = {18-23},
Year = {1981},
Month = {June},
ISSN = {0148-4834},
Keywords = {Communication* • Education, Nursing, Baccalaureate*
• Humans • Interpersonal Relations • Nursing,
Supervisory* • Psychiatric Nursing • Students,
Nursing* • Teaching* • education},
Language = {eng},
Key = {fds174099}
}
@article{fds132760,
Author = {JH Maki, JR MacFall and GA Johnson},
Title = {The use of gradient flow compensation to separate diffusion
and microcirculatory flow in MRI.},
Journal = {Magnetic resonance in medicine : official journal of the
Society of Magnetic Resonance in Medicine / Society of
Magnetic Resonance in Medicine, UNITED STATES},
Volume = {17},
Number = {1},
Pages = {95-107},
Year = {1991},
Month = {January},
ISSN = {0740-3194},
Keywords = {Acetone • Animals • Brain • Dextrans •
Diffusion • Edetic Acid • Female • Gadolinium
• Gels • Image Enhancement • Magnetic
Resonance Imaging • Microcirculation • Models,
Biological • Models, Structural • Rats •
Rats, Inbred F344 • Reproducibility of Results •
Rheology • Water • blood supply • chemistry
• instrumentation • methods* •
physiology*},
Abstract = {This paper describes a new MR imaging technique termed
Modified Stejskal Tanner versus Flow Compensation (MST/FC)
for the separation of diffusion and microcirculatory flow.
The theory behind the sequence is explained, along with a
five-component model of microcirculation applicable to any
"perfusion" imaging technique. Phantom data is presented
showing that (1) diffusion effects can be matched between
MST and FC (suggesting the possibility of flow-compensated
diffusion imaging), and (2) the technique is a quantitative
method of separating diffusion and slow (less than 0.25
mm/s) tortuous flow through a Sephadex column. Furthermore,
animal images show the technique to be feasible and
quantitative in measuring rat brain microcirculation under
normal, vasodilated (hypercarbia), and no-flow (post mortem)
conditions.},
Key = {fds132760}
}
@booklet{Maki91b,
Author = {Maki, JH and MacFall, JR and Johnson, GA},
Title = {The use of gradient flow compensation to separate diffusion
and microcirculatory flow in MRI.},
Journal = {Magn Reson Med},
Volume = {17},
Number = {1},
Pages = {95-107},
Year = {1991},
Month = {January},
ISSN = {0740-3194},
url = {http://www.ncbi.nlm.nih.gov/pubmed/1712421},
Abstract = {This paper describes a new MR imaging technique termed
Modified Stejskal Tanner versus Flow Compensation (MST/FC)
for the separation of diffusion and microcirculatory flow.
The theory behind the sequence is explained, along with a
five-component model of microcirculation applicable to any
"perfusion" imaging technique. Phantom data is presented
showing that (1) diffusion effects can be matched between
MST and FC (suggesting the possibility of flow-compensated
diffusion imaging), and (2) the technique is a quantitative
method of separating diffusion and slow (less than 0.25
mm/s) tortuous flow through a Sephadex column. Furthermore,
animal images show the technique to be feasible and
quantitative in measuring rat brain microcirculation under
normal, vasodilated (hypercarbia), and no-flow (post mortem)
conditions.},
Doi = {10.1002/mrm.1910170114},
Key = {Maki91b}
}
@article{fds268740,
Author = {Pandit, P and Johnston, SM and Qi, Y and Story, J and Nelson, R and Johnson, GA},
Title = {The utility of micro-CT and MRI in the assessment of
longitudinal growth of liver metastases in a preclinical
model of colon carcinoma.},
Journal = {Acad Radiol},
Volume = {20},
Number = {4},
Pages = {430-439},
Year = {2013},
Month = {April},
url = {http://www.ncbi.nlm.nih.gov/pubmed/23498983},
Abstract = {RATIONALE AND OBJECTIVES: Liver is a common site for distal
metastases in colon and rectal cancer. Numerous clinical
studies have analyzed the relative merits of different
imaging modalities for detection of liver metastases.
Several exciting new therapies are being investigated in
preclinical models. But, technical challenges in preclinical
imaging make it difficult to translate conclusions from
clinical studies to the preclinical environment. This study
addresses the technical challenges of preclinical magnetic
resonance imaging (MRI) and micro-computed tomography (CT)
to enable comparison of state-of-the-art methods for
following metastatic liver disease. MATERIALS AND METHODS:
We optimized two promising preclinical protocols to enable a
parallel longitudinal study tracking metastatic human colon
carcinoma growth in a mouse model: T2-weighted MRI using
two-shot PROPELLER (Periodically Rotated Overlapping
ParallEL Lines with Enhanced Reconstruction) and
contrast-enhanced micro-CT using a liposomal contrast agent.
Both methods were tailored for high throughput with
attention to animal support and anesthesia to limit
biological stress. RESULTS AND CONCLUSIONS: Each modality
has its strengths. Micro-CT permitted more rapid acquisition
(<10 minutes) with the highest spatial resolution (88-micron
isotropic resolution). But detection of metastatic lesions
requires the use of a blood pool contrast agent, which could
introduce a confound in the evaluation of new therapies. MRI
was slower (30 minutes) and had lower anisotropic spatial
resolution. But MRI eliminates the need for a contrast agent
and the contrast-to-noise between tumor and normal
parenchyma was higher, making earlier detection of small
lesions possible. Both methods supported a relatively
high-throughput, longitudinal study of the development of
metastatic lesions.},
Doi = {10.1016/j.acra.2012.09.030},
Key = {fds268740}
}
@booklet{Mellin94,
Author = {Mellin, AF and Cofer, GP and Smith, BR and Suddarth, SA and Hedlund, LW and Johnson, GA},
Title = {Three dimensional magnetic resonance microangiography of rat
neurovasculature.},
Journal = {Magn Reson Med},
Volume = {32},
Number = {2},
Pages = {199-205},
Year = {1994},
Month = {August},
ISSN = {0740-3194},
url = {http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:A1994NZ79300007&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=47d3190e77e5a3a53558812f597b0b92},
Abstract = {Techniques are described to perform three dimensional (3D)
MR microangiography. We have combined the use of a blood
pool agent (Gd-DTPA-complexed with bovine serum albumin),
three dimensional Fourier encoding, careful animal
stabilization, and volume rendering to permit imaging with
voxels of 60 x 60 x 60 microns. 3DFT encoding has been
performed at 7.1 T with very large arrays (256 x 512 x 512).
Interactive volume rendering allows a number of unique
display opportunities that effectively exploit these
isotropic 3D arrays.},
Doi = {10.1002/mrm.1910320208},
Key = {Mellin94}
}
@booklet{Stern89,
Author = {Stern, RL and Cline, HE and Johnson, GA and Ravin,
CE},
Title = {Three-dimensional imaging of the thoracic
cavity.},
Journal = {Invest Radiol},
Volume = {24},
Number = {4},
Pages = {282-288},
Year = {1989},
Month = {April},
ISSN = {0020-9996},
url = {http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:A1989U232200004&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=47d3190e77e5a3a53558812f597b0b92},
Abstract = {Three-dimensional (3D) surface reconstruction techniques
were applied to sets of computed tomographic (CT) images of
the thoracic cavity. Emphasis was placed on extracting lung
images. High quality, detailed 3D images of the lung surface
and internal bronchial and vascular structures were
produced.},
Doi = {10.1097/00004424-198904000-00005},
Key = {Stern89}
}
@article{9082523,
Author = {Oldham, M. and Sakhalkar, H. and Oliver, T. and Ying Min
Wang and Kirpatrick, J. and Yiting Cao and Badea, C. and Johnson, G.A. and Dewhirst, M.},
Title = {Three-dimensional imaging of xenograft tumors using optical
computed and emission tomography},
Journal = {Med. Phys. (USA)},
Volume = {33},
Number = {9},
Pages = {3193 - 202},
url = {http://dx.doi.org/10.1118/1.2217109},
Keywords = {bio-optics;biomedical optical imaging;cancer;cellular
biophysics;computerised tomography;image
reconstruction;proteins;tumours;},
Abstract = {The physical basis and preliminary applications of optical
computed tomography (optical-CT) and optical emission
computed tomography (optical-ECT) are introduced, as new
techniques with potential to provide unique 3D information
on a variety of aspects of tumor structure and function. A
particular focus here is imaging tumor micro-vasculature,
and the spatial distribution of viable tumor cells, although
the techniques have the potential for much wider
application. The principle attractiveness of optical-CT and
optical-ECT are that high resolution (<20 μm) and high
contrast co-registered 3D images of structure and function
can be acquired for relatively large intact samples. The
unique combination of high contrast and resolution offers
advantages over micro-CT and micro-MRI, and the lack of
requirement for sectioning offers advantages over confocal
microscopy, conventional microscopy, and histological
sectioning techniques. Optical-CT/ECT are implemented using
in-house custom apparatus and a commercial dissecting
microscope capable of both transmission and fluorescence
imaging. Basic studies to characterize imaging performance
are presented. Negligible geometrical distortion and
accurate reconstruction of relative attenuation coefficients
was observed. Optical-CT and optical-ECT are investigated
here by application to high resolution imaging of HCT116
xenograft tumors, about 1 cc in dimension, which were
transfected with constitutive red fluorescent protein (RFP).
Tumor microvasculature was stained in vivo by tail vein
injection of either passive absorbing dyes or active
fluorescent markers (FITC conjugated lectin). Prior to
imaging, the tumors were removed (ex vivo) and optically
cleared in a key process to make the samples amenable to
light transmission. The cleared tumors were imaged in three
modes (i) optical-CT to image the 3D distribution of
microvasculature as indicated by absorbing dye, (ii)
optical-ECT using the FITC excitation and emission filter
set, to determine microvasculature as indicated by
lectin-endothelial binding, and (iii) optical-ECT using the
DSRed2 filter set to determine the 3D distribution of viable
tumor as indicated by RFP emission. A clear correlation was
observed between the independent vasculature imaging modes
(i) and (ii) and postimaging histological sections,
providing substantial validation of the optical-CT and
optical-ECT techniques. Strong correlation was also observed
between the RFP imaging of mode iii, and modes i and ii,
supporting the intuitive conclusion that well-perfused
regions contain significant viable tumor. In summary,
optical-CT and optical-ECT, when combined with new optical
clearing techniques, represent powerful new imaging
modalities with potential for providing unique information
on the structure and function of tumors},
Key = {9082523}
}
@article{fds268877,
Author = {Oldham, M and Sakhalkar, H and Oliver, T and Wang, YM and Kirpatrick, J and Cao, Y and Badea, C and Johnson, GA and Dewhirst,
M},
Title = {Three-dimensional imaging of xenograft tumors using optical
computed and emission tomography.},
Journal = {Med Phys},
Volume = {33},
Number = {9},
Pages = {3193-3202},
Year = {2006},
Month = {September},
ISSN = {0094-2405},
url = {http://www.ncbi.nlm.nih.gov/pubmed/17022212},
Keywords = {Animals • Cell Line, Tumor • Colonic Neoplasms
• Humans • Image Enhancement • Image
Interpretation, Computer-Assisted • Imaging,
Three-Dimensional • Mice • Microscopy •
Reproducibility of Results • Sensitivity and
Specificity • Subtraction Technique* • Tomography,
Emission-Computed, Single-Photon • Tomography, Optical
• Tomography, X-Ray Computed • instrumentation
• methods* • pathology*},
Abstract = {The physical basis and preliminary applications of optical
computed tomography (optical-CT) and optical emission
computed tomography (optical-ECT) are introduced, as new
techniques with potential to provide unique 3D information
on a variety of aspects of tumor structure and function. A
particular focus here is imaging tumor micro-vasculature,
and the spatial distribution of viable tumor cells, although
the techniques have the potential for much wider
application. The principle attractiveness of optical-CT and
optical-ECT are that high resolution (<20 microm) and high
contrast co-registered 3D images of structure and function
can be acquired for relatively large intact samples. The
unique combination of high contrast and resolution offers
advantages over micro-CT and micro-MRI, and the lack of
requirement for sectioning offers advantages over confocal
microscopy, conventional microscopy, and histological
sectioning techniques. Optical-CT/ECT are implemented using
in-house custom apparatus and a commercial dissecting
microscope capable of both transmission and fluorescence
imaging. Basic studies to characterize imaging performance
are presented. Negligible geometrical distortion and
accurate reconstruction of relative attenuation coefficients
was observed. Optical-CT and optical-ECT are investigated
here by application to high resolution imaging of HCT116
xenograft tumors, about 1 cc in dimension, which were
transfected with constitutive red fluorescent protein (RFP).
Tumor microvasculature was stained in vivo by tail vein
injection of either passive absorbing dyes or active
fluorescent markers (FITC conjugated lectin). Prior to
imaging, the tumors were removed (ex vivo) and optically
cleared in a key process to make the samples amenable to
light transmission. The cleared tumors were imaged in three
modes (i) optical-CT to image the 3D distribution of
microvasculature as indicated by absorbing dye, (ii)
optical-ECT using the FITC excitation and emission filter
set, to determine microvasculature as indicated by
lectin-endothelial binding, and (iii) optical-ECT using the
DSRed2 filter set to determine the 3D distribution of viable
tumor as indicated by RFP emission. A clear correlation was
observed between the independent vasculature imaging modes
(i) and (ii) and postimaging histological sections,
providing substantial validation of the optical-CT and
optical-ECT techniques. Strong correlation was also observed
between the RFP imaging of mode iii, and modes i and ii,
supporting the intuitive conclusion that well-perfused
regions contain significant viable tumor. In summary,
optical-CT and optical-ECT, when combined with new optical
clearing techniques, represent powerful new imaging
modalities with potential for providing unique information
on the structure and function of tumors.},
Doi = {10.1118/1.2217109},
Key = {fds268877}
}
@booklet{Bone86,
Author = {Bone, SN and Johnson, GA and Thompson, MB},
Title = {Three-dimensional magnetic resonance microscopy of the
developing chick embryo.},
Journal = {Invest Radiol},
Volume = {21},
Number = {10},
Pages = {782-787},
Year = {1986},
Month = {October},
ISSN = {0020-9996},
url = {http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:A1986E347800003&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=47d3190e77e5a3a53558812f597b0b92},
Abstract = {Magnetic resonance imaging microscopy was performed on live
chick embryos. A combination of high gradient strength (0.47
mT/cm), special purpose radiofrequency coils and
3-dimensional Fourier imaging was used to obtain images with
effective thickness of 1.25 mm and pixel dimensions as small
as 200 mu in the live chick embryo. The signal-to-noise
ratio was sufficient to allow unequivocal identification of
the individual chambers of the heart, spinal cord,
ventricles in the brain, and vascular structures in the
liver of a live 11-day embryo. Anatomical assignment was
accomplished with the aid of correlated histologic sections.
Because there are no external landmarks, the plane of
imaging is frequently oblique, making the 3-dimensional
acquisition particularly useful.},
Doi = {10.1097/00004424-198610000-00003},
Key = {Bone86}
}
@booklet{Suddarth91,
Author = {Suddarth, SA and Johnson, GA},
Title = {Three-dimensional MR microscopy with large
arrays.},
Journal = {Magn Reson Med},
Volume = {18},
Number = {1},
Pages = {132-141},
Year = {1991},
Month = {March},
ISSN = {0740-3194},
url = {http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:A1991EZ54700013&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=47d3190e77e5a3a53558812f597b0b92},
Abstract = {MR microscopy of fixed specimens is described using large
(256(3] arrays. Images are acquired at 7.0 T with voxels as
small as 70 x 70 x 70 microns (3.4 x 10(-4) mm3), more than
25,000 times smaller than routine clinical body imaging.
Separation of the acquisition, reconstruction, archival, and
analysis onto networked workstations provides flexibility
and efficiency in handling the large data sets. The
isotropic data can be interactively displayed through any
plane without loss of in-plane resolution. The potential for
applications of MR microscopy in clinical pathology is
addressed.},
Doi = {10.1002/mrm.1910180114},
Key = {Suddarth91}
}
@booklet{Johnson87e,
Author = {Johnson, GA and Thompson, MB and Drayer, BP},
Title = {Three-dimensional MRI microscopy of the normal rat
brain.},
Journal = {Magn Reson Med},
Volume = {4},
Number = {4},
Pages = {351-365},
Year = {1987},
Month = {April},
ISSN = {0740-3194},
url = {http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:A1987G775900005&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=47d3190e77e5a3a53558812f597b0b92},
Abstract = {Magnetic resonance imaging techniques have been developed to
enable imaging of the live rat brain with thin (1.2-mm)
slices and microscopic pixels (115 X 115 microns).
Signal-to-noise ratios high enough to realize the
microscopic resolution are obtained with rf coils designed
for the subject and through the use of three-dimensional
Fourier spin warp imaging. The technique yields 16
contiguous slices. Correlation with fixed pathologic
specimens enables unequivocal identification of gray and
white matter structures in the brain of a live 200-g rat.
Structures clearly visible in the MR images include Ammon's
horn, the hypothalamus, corpus callosum and substantia
nigra, as well as a number of brainstem nuclei.},
Doi = {10.1002/mrm.1910040406},
Key = {Johnson87e}
}
@article{fds268835,
Author = {Zhang, X and Badea, CT and Johnson, GA},
Title = {Three-dimensional reconstruction in free-space whole-body
fluorescence tomography of mice using optically
reconstructed surface and atlas anatomy.},
Journal = {J Biomed Opt},
Volume = {14},
Number = {6},
Pages = {064010},
Year = {2009},
ISSN = {1560-2281},
url = {http://www.ncbi.nlm.nih.gov/pubmed/20059248},
Keywords = {Algorithms* • Animals • Image Processing,
Computer-Assisted • Imaging, Three-Dimensional •
Mice • Mice, Nude • Phantoms, Imaging •
Spectrometry, Fluorescence • Thermography •
Tomography • Whole Body Imaging • X-Ray
Microtomography • methods • methods*},
Abstract = {We present a 3-D image reconstruction method for free-space
fluorescence tomography of mice using hybrid anatomical
prior information. Specifically, we use an optically
reconstructed surface of the experimental animal and a
digital mouse atlas to approximate the anatomy of the animal
as structural priors to assist image reconstruction.
Experiments are carried out on a cadaver of a nude mouse
with a fluorescent inclusion (2.4-mm-diam cylinder)
implanted in the chest cavity. Tomographic fluorescence
images are reconstructed using an iterative algorithm based
on a finite element method. Coregistration of the
fluorescence reconstruction and micro-CT (computed
tomography) data acquired afterward show good localization
accuracy (localization error 1.2+/-0.6 mm). Using the
optically reconstructed surface, but without the atlas
anatomy, image reconstruction fails to show the fluorescent
inclusion correctly. The method demonstrates the utility of
anatomical priors in support of free-space fluorescence
tomography.},
Language = {eng},
Doi = {10.1117/1.3258836},
Key = {fds268835}
}
@booklet{Johnson81c,
Author = {G. A. Johnson and J. J. Mcnamara},
Title = {Thromboxane-a2 - a vital mediator in the acute response to
hemorrhage},
Journal = {Circulation},
Volume = {64},
Number = {4},
Pages = {290 -- 290},
Year = {1981},
Key = {Johnson81c}
}
@booklet{Sostman85,
Author = {Sostman, HD and Gore, JC and Flye, MW and Johnson, GA and Herfkens,
RJ},
Title = {Time course and mechanism of alterations in proton
relaxation during liver regeneration in the
rat.},
Journal = {Hepatology},
Volume = {5},
Number = {4},
Pages = {538-543},
Year = {1985},
ISSN = {0270-9139},
url = {http://www.ncbi.nlm.nih.gov/pubmed/2991104},
Abstract = {We studied the proton T1 and T2, water and lipid content of
regenerating rat liver from 1 to 7 days after 70%
hepatectomy. Liver from normal and sham-operated animals and
splenic tissue from all animals were studied as controls. In
vivo proton spectroscopy and imaging of liver was performed
in a separate group of control and posthepatectomy rats. The
T2 of regenerating liver, but not of sham or normal control
liver, was prolonged. Changes in T1, relative to normal
tissue, were found in liver and spleen of both operated
groups. Lipid content, assessed both by extraction of tissue
samples and by in vivo spectroscopy, was increased in
regenerating tissue but not in controls. Water content was
similarly increased in regenerating liver tissue. Changes in
water and lipid content appeared to contribute to the
alterations in proton relaxation which we
observed.},
Doi = {10.1002/hep.1840050403},
Key = {Sostman85}
}
@article{fds132783,
Author = {HD Sostman and JC Gore and MW Flye and GA Johnson and RJ
Herfkens},
Title = {Time course and mechanism of alterations in proton
relaxation during liver regeneration in the
rat.},
Journal = {Hepatology (Baltimore, Md.), UNITED STATES},
Volume = {5},
Number = {4},
Pages = {538-43},
ISSN = {0270-9139},
Keywords = {Animals • Body Water • Lipids • Liver •
Liver Regeneration* • Magnetic Resonance Spectroscopy
• Male • Protons • Rats • Rats, Inbred
Strains • Spectrum Analysis • Spleen • Time
Factors • analysis • anatomy & histology •
anatomy & histology* • diagnostic use* •
methods},
Abstract = {We studied the proton T1 and T2, water and lipid content of
regenerating rat liver from 1 to 7 days after 70%
hepatectomy. Liver from normal and sham-operated animals and
splenic tissue from all animals were studied as controls. In
vivo proton spectroscopy and imaging of liver was performed
in a separate group of control and posthepatectomy rats. The
T2 of regenerating liver, but not of sham or normal control
liver, was prolonged. Changes in T1, relative to normal
tissue, were found in liver and spleen of both operated
groups. Lipid content, assessed both by extraction of tissue
samples and by in vivo spectroscopy, was increased in
regenerating tissue but not in controls. Water content was
similarly increased in regenerating liver tissue. Changes in
water and lipid content appeared to contribute to the
alterations in proton relaxation which we
observed.},
Key = {fds132783}
}
@booklet{Smith98a,
Author = {B. R. Smith and M. D. Shattuck and L. W. Hedlund and G. A.
Johnson},
Title = {Time-course imaging of rat embryos in utero with magnetic
resonance microscopy},
Journal = {Magnetic Resonance In Medicine},
Volume = {39},
Number = {4},
Pages = {673 -- 677},
Year = {1998},
Month = {April},
Key = {Smith98a}
}
@booklet{Smith98,
Author = {Smith, BR and Sattuck, MD and Hedlund, LW and Johnson,
GA},
Title = {Time-course imaging of rat embryos in utero with magnetic
resonance microscopy (vol 39, pg 673, 1998)},
Journal = {Magnetic Resonance In Medicine},
Volume = {39},
Number = {6},
Pages = {CP2-CP2},
Year = {1998},
Month = {June},
Key = {Smith98}
}
@article{fds268905,
Author = {Smith, BR and Shattuck, MD and Hedlund, LW and Johnson,
GA},
Title = {Time-course imaging of rat embryos in utero with magnetic
resonance microscopy.},
Journal = {Magn Reson Med},
Volume = {39},
Number = {4},
Pages = {673-677},
Year = {1998},
Month = {April},
ISSN = {0740-3194},
url = {http://www.ncbi.nlm.nih.gov/pubmed/9543433},
Keywords = {Animals • Embryo • Embryonic and Fetal Development
• Female • Fetus • Follow-Up Studies •
Gestational Age • Image Processing, Computer-Assisted
• Magnetic Resonance Imaging • Pregnancy •
Rats • Rats, Sprague-Dawley • Time Factors •
Uterus • anatomy & histology • anatomy &
histology* • methods*},
Abstract = {Magnetic resonance (MR) microscopy was used to noninvasively
investigate the development of live rat embryos in utero.
The difficulty in making sequential observations of a
developing mammalian embryo has frustrated developmental
biologists for many years. Most current technologies analyze
normal and abnormal development by observing end point
phenotypes (in fixed specimens) rather than investigating
the live embryo. MR microscopy was adapted to allow rat
litters to be scanned three times each (at 1- to 3-day
intervals) and has produced images of live developing
embryos. It was demonstrated that repeated anesthesia and
imaging protocols produced no gross malformations in the rat
pups that were subsequently delivered and observed.
Three-dimensional projection encoding with phase rewinders
produced isotropic [256(3)] image data sets in about 30
minutes with excellent tissue contrast arising from
steady-state effects in the amniotic fluid.},
Doi = {10.1002/mrm.1910390424},
Key = {fds268905}
}
@booklet{Baxter91,
Author = {BAXTER, GW and LEONE, R and JOHNSON, GA and BEHRINGER,
RP},
Title = {TIME-DEPENDENCE, SCALING AND PATTERN-FORMATION FOR FLOWING
SAND},
Journal = {EUROPEAN JOURNAL OF MECHANICS B-FLUIDS},
Volume = {10},
Number = {2},
Pages = {181-186},
Publisher = {GAUTHIER-VILLARS},
Year = {1991},
Month = {January},
ISSN = {0997-7546},
url = {http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:A1991FR81800029&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=47d3190e77e5a3a53558812f597b0b92},
Key = {Baxter91}
}
@article{fds132807,
Author = {GA Johnson and RJ Herfkens and MA Brown},
Title = {Tissue relaxation time: in vivo field dependence.},
Journal = {Radiology, UNITED STATES},
Volume = {156},
Number = {3},
Pages = {805-10},
Year = {1985},
Month = {September},
ISSN = {0033-8419},
Keywords = {Adipose Tissue • Animals • Dogs • Electron
Spin Resonance Spectroscopy* • Kidney Cortex •
Magnetic Resonance Spectroscopy* • Muscles •
Spleen • anatomy & histology},
Abstract = {Relaxation times (T1 and T2) were measured in vivo in
mongrel dogs at fields of 0.3, 0.5, 1.0, 1.35, and 1.5 tesla
(T). T1 was measured using nine values of inversion time
ranging from 10 to 1,280 msec. T2 was measured with a
four-point multiple spin-echo sequence. Relaxation times
were calculated for muscle, kidney cortex, spleen, and
adipose tissue. T2 is independent of field. A linear fit to
the field dependence of T1 yields slopes of 400-500 msec/T
for tissues in which the primary source of protons is water.
The lower slope of adipose (approximately 150 msec/T)
reflects the different mechanism of spin-lattice relaxation
of the -CH2 protons.},
Key = {fds132807}
}
@booklet{Johnson85,
Author = {Johnson, GA and Herfkens, RJ and Brown, MA},
Title = {Tissue relaxation time: in vivo field dependence.},
Journal = {Radiology},
Volume = {156},
Number = {3},
Pages = {805-810},
Year = {1985},
Month = {September},
ISSN = {0033-8419},
url = {http://www.ncbi.nlm.nih.gov/pubmed/2991980},
Abstract = {Relaxation times (T1 and T2) were measured in vivo in
mongrel dogs at fields of 0.3, 0.5, 1.0, 1.35, and 1.5 tesla
(T). T1 was measured using nine values of inversion time
ranging from 10 to 1,280 msec. T2 was measured with a
four-point multiple spin-echo sequence. Relaxation times
were calculated for muscle, kidney cortex, spleen, and
adipose tissue. T2 is independent of field. A linear fit to
the field dependence of T1 yields slopes of 400-500 msec/T
for tissues in which the primary source of protons is water.
The lower slope of adipose (approximately 150 msec/T)
reflects the different mechanism of spin-lattice relaxation
of the -CH2 protons.},
Doi = {10.1148/radiology.156.3.2991980},
Key = {Johnson85}
}
@article{fds268863,
Author = {Badea, CT and Hedlund, LW and De Lin and M and Mackel, JSB and Samei, E and Johnson, GA},
Title = {Tomographic digital subtraction angiography for lung
perfusion estimation in rodents.},
Journal = {Med Phys},
Volume = {34},
Number = {5},
Pages = {1546-1555},
Year = {2007},
Month = {May},
ISSN = {0094-2405},
url = {http://www.ncbi.nlm.nih.gov/pubmed/17555236},
Keywords = {Angiography, Digital Subtraction • Animals •
Female • Lung • Magnetic Resonance Angiography
• Perfusion • Radiographic Image Enhancement
• Rats • Rats, Inbred F344 • Tomography,
X-Ray Computed • blood supply • methods •
methods* • radiography*},
Abstract = {In vivo measurements of perfusion present a challenge to
existing small animal imaging techniques such as magnetic
resonance microscopy, micro computed tomography, micro
positron emission tomography, and microSPECT, due to
combined requirements for high spatial and temporal
resolution. We demonstrate the use of tomographic digital
subtraction angiography (TDSA) for estimation of perfusion
in small animals. TDSA augments conventional digital
subtraction angiography (DSA) by providing three-dimensional
spatial information using tomosynthesis algorithms. TDSA is
based on the novel paradigm that the same time density
curves can be reproduced in a number of consecutive
injections of microL volumes of contrast at a series of
different angles of rotation. The capabilities of TDSA are
established in studies on lung perfusion in rats. Using an
imaging system developed in-house, we acquired data for
four-dimensional (4D) imaging with temporal resolution of
140 ms, in-plane spatial resolution of 100 microm, and slice
thickness on the order of millimeters. Based on a structured
experimental approach, we optimized TDSA imaging providing a
good trade-off between slice thickness, the number of
injections, contrast to noise, and immunity to artifacts.
Both DSA and TDSA images were used to create parametric maps
of perfusion. TDSA imaging has potential application in a
number of areas where functional perfusion measurements in
4D can provide valuable insight into animal models of
disease and response to therapeutics.},
Doi = {10.1118/1.2717384},
Key = {fds268863}
}
@article{064710257107,
Author = {Johnson, G.A. and Davis, J.G. and Qian, Y.L. and Doesken,
K.C.},
Title = {Topdressing turf with composted manure improves soil quality
and protects water quality},
Journal = {Soil Science Society of America Journal},
Volume = {70},
Number = {6},
Pages = {2114 - 2121},
Year = {2006},
url = {http://dx.doi.org/10.2136/sssaj2005.0287},
Keywords = {Manures;Hydraulic conductivity;Sediments;},
Abstract = {Compost can improve soil properties when incorporated into
soil; however, little information is available regarding
impacts of compost topdressing. Objectives of this study
were to evaluate the effects that topdressing composted
dairy manure onto Kentucky bluegrass (Poa pratensis L.) has
on: (i) soil physical properties, (ii) soil chemical
properties, (iii) soil nitrate (NOs-N) and P concentrations
below the rootzone, (iv) total runoff and sediment losses,
and (v) N and P concentrations in runoff. Plots were
topdressed with compost at 0, 33, 66, and 99 m<sup>3</sup>
ha<sup>-1</sup>. Saturated hydraulic conductivity, bulk
density, water retention, and soil nutrient levels were
measured. A rainfall simulation was conducted, and runoff
was collected and analyzed for total nitrogen (TN), nitrate
nitrogen (NO<sub>3</sub>-N), ammonium nitrogen
(NH<sub>4</sub>-N), total phosphorus (TP), total dissolved P
(TDP), and orthophosphate (OP). Compost application of 99
m<sup>3</sup> ha<sup>-1</sup> reduced bulk density, and
increased water retention and P, K, Fe, and Mn
concentrations in the surface soil. Compost applications of
66 m<sup>3</sup> ha<sup>-1</sup> or greater raised soil
electrical conductivity (EC); however, this increase i n
soil EC did not negatively impact turf quality. Rates of
runoff and erosion and concentrations of TN,
NO<sub>3</sub>-N, TP, TDP, and OP in runoff were not
different among treatments. However, all compost treatments
did increase NH<sub>4</sub>-N concentrations in runoff.
There were no differences in soil NO<sub>3</sub>-N or
available P levels below the root zone. Topdressing
composted manure onto established turf improved soil
physical properties and nutrient concentrations without
increasing nutrient runoff, with the exception of increased
NH<sub>4</sub>-N levels in runoff. © Soil Science
Society of America.},
Key = {064710257107}
}
@article{fds174217,
Author = {GA Johnson and C Wilken and EA Van Kirk and EL Belden and WJ
Murdoch},
Title = {Toward regulation of gonadal function by a synthetic hybrid
molecule composed of gonadotropin and Fc fragment of
immunoglobulin G.},
Journal = {American journal of reproductive immunology (New York, N.Y.
: 1989)},
Volume = {23},
Number = {1},
Pages = {22-5},
Year = {1990},
Month = {May},
ISSN = {1046-7408},
Keywords = {Animals • Cell Survival • Chorionic Gonadotropin
• Cross-Linking Reagents • Immunoglobulin Fc
Fragments • Immunoglobulin G • Leydig Cells •
Luteinizing Hormone • Male • Reproduction •
Sheep • Testis • Testosterone • Tumor Cells,
Cultured • blood • drug effects • drug
effects* • immunology • metabolism •
pharmacology*},
Abstract = {A conjugate of human chorionic gonadotropin (HCG) and Fc
fragment of immunoglobulin G was prepared by covalent
cross-linking using the heterobifunctional reagent,
N-succinimidyl 3-(2-pyridyldithio) propionate. Mouse Leydig
tumor cells expressing receptors for luteinizing hormone
were specifically lysed in vitro as a consequence of
complement fixation via the Fc component of the hybrid
molecule. Furthermore, administration of HCG-Fc to rams
caused an acute depression in circulatory testosterone. This
novel concept of targeted inhibition of gonadal function
could prove to have future applications in control of
reproductive processes.},
Language = {eng},
Key = {fds174217}
}
@booklet{Johnson90,
Author = {G. A. Johnson and C. Wilken and E. A. Vankirk and E. L.
Belden and W. J. Murdoch},
Title = {Toward regulation of gonadal-function by a synthetic hybrid
molecule composed of gonadotropin and fc fragment of
immunoglobulin-g},
Journal = {American Journal Of Reproductive Immunology},
Volume = {23},
Number = {1},
Pages = {22 -- 25},
Year = {1990},
Month = {May},
Key = {Johnson90}
}
@article{fds364084,
Author = {Shen, J and Zhao, Q and Qi, Y and Cofer, G and Johnson, GA and Wang,
N},
Title = {Tractography of Porcine Meniscus Microstructure Using
High-Resolution Diffusion Magnetic Resonance
Imaging.},
Journal = {Front Endocrinol (Lausanne)},
Volume = {13},
Pages = {876784},
Year = {2022},
url = {http://dx.doi.org/10.3389/fendo.2022.876784},
Abstract = {To noninvasively evaluate the three-dimensional collagen
fiber architecture of porcine meniscus using diffusion MRI,
meniscal specimens were scanned using a 3D
diffusion-weighted spin-echo pulse sequence at 7.0 T. The
collagen fiber alignment was revealed in each voxel and the
complex 3D collagen network was visualized for the entire
meniscus using tractography. The proposed automatic
segmentation methods divided the whole meniscus to different
zones (Red-Red, Red-White, and White-White) and different
parts (anterior, body, and posterior). The diffusion tensor
imaging (DTI) metrics were quantified based on the
segmentation results. The heatmap was generated to
investigate the connections among different regions of
meniscus. Strong zonal-dependent diffusion properties were
demonstrated by DTI metrics. The fractional anisotropy (FA)
value increased from 0.13 (White-White zone) to 0.26
(Red-Red zone) and the radial diffusivity (RD) value changed
from 1.0 × 10-3 mm2/s (White-White zone) to 0.7 × 10-3
mm2/s (Red-Red zone). Coexistence of both radial and
circumferential collagen fibers in the meniscus was evident
by diffusion tractography. Weak connections were found
between White-White zone and Red-Red zone in each part of
the meniscus. The anterior part and posterior part were less
connected, while the body part showed high connections to
both anterior part and posterior part. The tractography
based on diffusion MRI may provide a complementary method to
study the integrity of meniscus and nondestructively
visualize the 3D collagen fiber architecture.},
Doi = {10.3389/fendo.2022.876784},
Key = {fds364084}
}
@booklet{Gunderson94,
Author = {R. O. Gunderson and M. Oprisan and G. A. Johnson and E. C.
Pfeiffer and D. L. Smith and A. W. Schneider and H. Volpe and B. Silverstein and R. J. Ringlee and T. E. Mcdermott and V. S. Rashkes and R. B. Adler and C. R. Heising and T. S.
White and M. G. Lau},
Title = {Transformer magnetizing current and iron-core losses in
harmonic power-flow - discussion},
Journal = {Ieee Transactions On Power Delivery},
Volume = {9},
Number = {1},
Pages = {34 -- 39},
Year = {1994},
Month = {January},
Key = {Gunderson94}
}
@article{fds174066,
Author = {DA Massuto and EC Kneese and GA Johnson and RC Burghardt and RN Hooper and NH Ing and LA Jaeger},
Title = {Transforming growth factor beta (TGFB) signaling is
activated during porcine implantation: proposed role for
latency-associated peptide interactions with integrins at
the conceptus-maternal interface.},
Journal = {Reproduction (Cambridge, England)},
Volume = {139},
Number = {2},
Pages = {465-78},
Year = {2010},
Month = {February},
ISSN = {1741-7899},
url = {http://dx.doi.org/10.1530/REP-09-0447},
Keywords = {Animals • Antigens, CD29 • Biotinylation •
Cell Line • Embryo Implantation* • Female •
Gestational Age • Immunohistochemistry • Integrin
beta Chains • Integrin beta3 • Integrins •
Peptides • Phosphorylation • Pregnancy •
Protein Binding • Protein Precursors • Receptors,
Transforming Growth Factor beta • Recombinant Proteins
• Signal Transduction* • Smad2 Protein •
Smad3 Protein • Swine • Transforming Growth Factor
beta • Trophoblasts • Uterus • metabolism
• metabolism*},
Abstract = {The process of implantation is mediated by a complex network
of signaling and adhesive factors. In the pig, latent and
active transforming growth factor beta (TGFB), TGFB
receptors (TGFBR), and integrins (ITGs) are present during
the peri-implantation period. TGFB signals via TGFBR and
activates downstream effector SMAD proteins 2 and 3
(p-SMAD2/3). Latency-associated peptide (LAP), part of the
latent TGFB complex, is known to bind to ITG heterodimers
and activate TGFB. We hypothesize that active TGFBs and
TGFBRs along with LAP and ITGs functionally interact at the
conceptus-maternal interface to mediate events essential for
conceptus development and attachment in pigs. Uteri and
conceptuses from days 10, 12, 16, 20, and 24 pregnant gilts
were immunostained for TGFB, LAP, and ITG subunits (ITGAV,
ITGB1, ITGB3, ITGB5, ITGB6, and ITGB8). Activation of TGFBRs
was evaluated by the presence of phosphorylated downstream
effector SMAD2/3. Binding of LAP to ITGs was also evaluated
using porcine trophectoderm cells. Abundant active TGFB was
detected at the apical surfaces of epithelia at the
conceptus-maternal interface, and p-SMAD2/3 was detected at
both conceptus attachment and nonattachment sites during
implantation. Separate aggregates of LAP, ITGB1, ITGB5, and
later ITGB3 were detected at the porcine conceptus-maternal
interface, and binding of LAP to ITGs on apical surfaces was
demonstrated. Results suggest that functional LAP-ITG
adhesion complexes support conceptus attachment and promote
TGFB activation leading to TGFB interaction with TGFBR
supporting events of porcine implantation.},
Language = {eng},
Doi = {10.1530/REP-09-0447},
Key = {fds174066}
}
@booklet{Hansen97,
Author = {T. R. Hansen and K. J. Austin and G. A. Johnson},
Title = {Transient ubiquitin cross-reactive protein gene expression
in the bovine endometrium},
Journal = {Endocrinology},
Volume = {138},
Number = {11},
Pages = {5079 -- 5082},
Year = {1997},
Month = {November},
Key = {Hansen97}
}
@article{fds174248,
Author = {TR Hansen and KJ Austin and GA Johnson},
Title = {Transient ubiquitin cross-reactive protein gene expression
in the bovine endometrium.},
Journal = {Endocrinology},
Volume = {138},
Number = {11},
Pages = {5079-82},
Year = {1997},
Month = {November},
ISSN = {0013-7227},
Keywords = {Animals • Blotting, Northern • Cattle •
Endometrium • Estrus • Female • Gene
Expression* • Glyceraldehyde-3-Phosphate Dehydrogenases
• Interferon Type I • Pregnancy • Pregnancy
Proteins • RNA, Messenger • Time Factors •
Transcription, Genetic • Ubiquitins • analogs &
derivatives* • drug effects • genetics •
metabolism • pharmacology • physiology*},
Abstract = {Bovine ubiquitin cross-reactive protein (boUCRP) is secreted
by the endometrium from days 15 to 26 of pregnancy in
response to conceptus-derived interferon-tau (IFN-tau). We
hypothesized that the gene encoding boUCRP was under
transcriptional control by the conceptus and IFN-tau.
Northern blots using radiolabeled UCRP cDNA revealed a
single UCRP transcript of approximately 700 b that was
present (P < 0.05) in endometrial cells cultured with 25 nM
rboIFN-tau. The UCRP mRNA was not detected in endometrium on
days 15, 17, 18 or 19 of the estrous cycle (n = 4 cows on
each day) or in spleen, kidney, liver, corpus luteum or
muscle. Bovine UCRP mRNA was detectable (P < 0.05) in
endometrium from pregnant cows by day 15, reached highest
levels by day 17, remained elevated on days 18, 19 and 21,
and then declined to amounts on day 26 that were not
detectable. Northern blot using radiolabeled ubiquitin cDNA
revealed presence of the two major ubiquitin transcripts UbB
(1.2 Kb) and UbC (2.6 Kb) in all tissues examined. The
bovine UCRP cDNA did not cross-hybridize with these
ubiquitin transcripts. We conclude that transcription of the
UCRP gene is transient during early pregnancy and regulated
by IFN-tau.},
Language = {eng},
Key = {fds174248}
}
@booklet{Brown84a,
Author = {Brown, MA and Johnson, GA},
Title = {Transition metal-chelate complexes as relaxation modifiers
in nuclear magnetic resonance.},
Journal = {Med Phys},
Volume = {11},
Number = {1},
Pages = {67-72},
Year = {1984},
ISSN = {0094-2405},
url = {http://www.ncbi.nlm.nih.gov/pubmed/6422223},
Abstract = {Studies are reported of relaxation modifiers for use in
nuclear magnetic resonance (NMR) imaging systems. Chelate
complexes of transition metal salts are under investigation
to determine their ability to reduce the spin-lattice
relaxation time (T1) of the nucleus under observation and to
reduce the toxicity of the metal ion. The
ethylenediaminetetraacetate (EDTA) complexes of FeCl3,
GdCl3, and MnCl2 are not as effective as the respective
salts in reducing T1 of water protons at 90 MHz. For Mn,
this diminution in ability is offset by a significant
reduction in toxicity. Explanations for this loss of
effectiveness are discussed.},
Doi = {10.1118/1.595455},
Key = {Brown84a}
}
@article{fds132821,
Author = {MA Brown and GA Johnson},
Title = {Transition metal-chelate complexes as relaxation modifiers
in nuclear magnetic resonance.},
Journal = {Medical physics, UNITED STATES},
Volume = {11},
Number = {1},
Pages = {67-72},
ISSN = {0094-2405},
Keywords = {Chlorides* • Edetic Acid • Ferric Compounds •
Gadolinium • Magnetic Resonance Spectroscopy* •
Manganese • Manganese Compounds*},
Abstract = {Studies are reported of relaxation modifiers for use in
nuclear magnetic resonance (NMR) imaging systems. Chelate
complexes of transition metal salts are under investigation
to determine their ability to reduce the spin-lattice
relaxation time (T1) of the nucleus under observation and to
reduce the toxicity of the metal ion. The
ethylenediaminetetraacetate (EDTA) complexes of FeCl3,
GdCl3, and MnCl2 are not as effective as the respective
salts in reducing T1 of water protons at 90 MHz. For Mn,
this diminution in ability is offset by a significant
reduction in toxicity. Explanations for this loss of
effectiveness are discussed.},
Key = {fds132821}
}
@article{fds204248,
Author = {G Wu and FW Bazer and GA Johnson and DA Knabe and RC Burghardt and TE
Spencer, XL Li and JJ Wang},
Title = {Triennial Growth Symposium: important roles for L-glutamine
in swine nutrition and production.},
Journal = {Journal of animal science},
Volume = {89},
Number = {7},
Pages = {2017-30},
Year = {2011},
Month = {July},
ISSN = {1525-3163},
url = {http://dx.doi.org/10.2527/jas.2010-3614},
Keywords = {Animal Feed • Animal Nutritional Physiological
Phenomena* • Animals • Diet • Dietary
Supplements • Female • Glutamine • Lactation
• Pregnancy • Swine • blood • growth &
development* • metabolism* • pharmacology •
veterinary*},
Abstract = {L-Glutamine (Gln) has traditionally not been considered a
nutrient needed in diets for livestock species or even
mentioned in classic animal nutrition textbooks. This is due
to previous technical difficulties in Gln analysis and the
unsubstantiated assumption that animals can synthesize
sufficient amounts of Gln to meet their needs. Consequently,
the current (1998) version of NRC does not recommend dietary
Gln requirements for swine. This lack of knowledge about Gln
nutrition has contributed to suboptimal efficiency of global
pig production. Because of recent advances in research, Gln
is now known to be an abundant AA in physiological fluids
and proteins and a key regulator of gene expression.
Additionally, Gln can regulate cell signaling via the
mammalian target of rapamycin pathway, adenosine
monophosphate-activated protein kinase, extracellular
signal-related kinase, Jun kinase, mitogen-activated protein
kinase, and nitric oxide. The exquisite integration of
Gln-dependent regulatory networks has profound effects on
cell proliferation, differentiation, migration, metabolism,
homeostasis, survival, and function. As a result of
translating basic research into practice, dietary
supplementation with 1% Gln maintains gut health and
prevents intestinal dysfunction in low-birth-weight or
early-weaned piglets while increasing their growth
performance and survival. In addition, supplementing 1% Gln
to a corn- and soybean-meal-based diet between d 90 and 114
of gestation ameliorates fetal growth retardation in gilts
and reduces preweaning mortality of piglets. Furthermore,
dietary supplementation with 1% Gln enhances milk production
by lactating sows. Thus, adequate amounts of dietary Gln, a
major nutrient, are necessary to support the maximum growth,
development, and production performance of
swine.},
Language = {eng},
Doi = {10.2527/jas.2010-3614},
Key = {fds204248}
}
@article{fds132759,
Author = {C Zimmer and SC Wright Jr and RT Engelhardt and GA Johnson and C Kramm and XO Breakefield and R Weissleder},
Title = {Tumor cell endocytosis imaging facilitates delineation of
the glioma-brain interface.},
Journal = {Experimental neurology},
Volume = {143},
Number = {1},
Pages = {61-9},
Year = {1997},
Month = {January},
ISSN = {0014-4886},
url = {http://dx.doi.org/10.1006/exnr.1996.6350},
Keywords = {Animals • Biological Markers • Brain Neoplasms
• Endocytosis* • Glioma • Image Processing,
Computer-Assisted • Rats • Tumor Cells, Cultured
• pathology*},
Abstract = {We describe a method for measuring tumor cell endocytosis in
vivo and provide the anatomic correlate of this tumor cell
function using a superparamagnetic and histologically
detectable marker for cell uptake (MION). Rats (n = 22) were
intrahemispherically implanted with a thymidine kinase
(TK)-positive 9L gliosarcoma cell line, where TK served as
the tumor marker. Twenty-four hours after intravenous
injection of 10 mg Fe/kg of MION, rat brains were removed
and underwent MR imaging ex vivo at near-microscopic
resolution (isotropic voxel size of 86 microm, 9.4 T) prior
to histologic processing. The imaging probe accumulated
within tumor cells adjacent to the hyperpermeable
tumor-brain interface including microscopic deposits and
along finger-like invasions of the tumor into brain,
facilitating the demarcation of the true histologic tumor
border in three dimensions by MR microscopy. The method has
potential research and clinical implications for delineating
the tumor-brain interface prior to therapy and/or for
providing a rational basis for imaging nanocolloid drug
delivery to solid tumors.},
Language = {eng},
Doi = {10.1006/exnr.1996.6350},
Key = {fds132759}
}
@booklet{Zimmer97,
Author = {Zimmer, C and Wright, SC and Engelhardt, RT and Johnson, GA and Kramm,
C and Breakefield, XO and Weissleder, R},
Title = {Tumor cell endocytosis imaging facilitates delineation of
the glioma-brain interface.},
Journal = {Exp Neurol},
Volume = {143},
Number = {1},
Pages = {61-69},
Year = {1997},
Month = {January},
ISSN = {0014-4886},
url = {http://dx.doi.org/10.1006/exnr.1996.6350},
Abstract = {We describe a method for measuring tumor cell endocytosis in
vivo and provide the anatomic correlate of this tumor cell
function using a superparamagnetic and histologically
detectable marker for cell uptake (MION). Rats (n = 22) were
intrahemispherically implanted with a thymidine kinase
(TK)-positive 9L gliosarcoma cell line, where TK served as
the tumor marker. Twenty-four hours after intravenous
injection of 10 mg Fe/kg of MION, rat brains were removed
and underwent MR imaging ex vivo at near-microscopic
resolution (isotropic voxel size of 86 microm, 9.4 T) prior
to histologic processing. The imaging probe accumulated
within tumor cells adjacent to the hyperpermeable
tumor-brain interface including microscopic deposits and
along finger-like invasions of the tumor into brain,
facilitating the demarcation of the true histologic tumor
border in three dimensions by MR microscopy. The method has
potential research and clinical implications for delineating
the tumor-brain interface prior to therapy and/or for
providing a rational basis for imaging nanocolloid drug
delivery to solid tumors.},
Doi = {10.1006/exnr.1996.6350},
Key = {Zimmer97}
}
@article{fds268860,
Author = {Badea, CT and Hedlund, LW and De Lin and M and Boslego Mackel and JF and Johnson, GA},
Title = {Tumor imaging in small animals with a combined
micro-CT/micro-DSA system using iodinated conventional and
blood pool contrast agents.},
Journal = {Contrast Media Mol Imaging},
Volume = {1},
Number = {4},
Pages = {153-164},
Year = {2006},
ISSN = {1555-4317},
url = {http://www.ncbi.nlm.nih.gov/pubmed/17193692},
Keywords = {Angiography, Digital Subtraction • Animals •
Contrast Media • Fibrosarcoma • Iodine • Rats
• Rats, Inbred F344 • Tomography, X-Ray Computed
• analysis • blood supply* • chemistry*
• instrumentation* • methods* •
radiography*},
Abstract = {X-ray based micro-computed tomography (CT) and micro-digital
subtraction angiography (DSA) are important non-invasive
imaging modalities for following tumorogenesis in small
animals. To exploit these imaging capabilities further, the
two modalities were combined into a single system to provide
both morphological and functional data from the same tumor
in a single imaging session. The system is described and
examples are given of imaging implanted fibrosarcoma tumors
in rats using two types of contrast media: (a) a new
generation of blood pool contrast agent containing iodine
with a concentration of 130 mg/mL (Fenestratrade mark VC,
Alerion Biomedical, San Diego, CA, USA) for micro-CT and (b)
a conventional iodinated contrast agent (Isovue(R)-370 mg/mL
iodine, trademark of Bracco Diagnostics, Princeton, NJ, USA)
for micro-DSA. With the blood pool contrast agent, the 3D
vascular architecture is revealed in exquisite detail at 100
microm resolution. Micro-DSA images, in perfect registration
with the 3D micro-CT datasets, provide complementary
functional information such as mean transit times and
relative blood flow through the tumor. This imaging approach
could be used to understand tumor angiogenesis better and be
the basis for evaluating anti-angiogenic
therapies.},
Doi = {10.1002/cmmi.103},
Key = {fds268860}
}
@article{fds292753,
Author = {Subashi, E and Cordero, FJ and Halvorson, KG and Qi, Y and Nouls, JC and Becher, OJ and Johnson, GA},
Title = {Tumor location, but not H3.3K27M, significantly influences
the blood-brain-barrier permeability in a genetic mouse
model of pediatric high-grade glioma.},
Journal = {J Neurooncol},
Volume = {126},
Number = {2},
Pages = {243-251},
Year = {2016},
Month = {January},
ISSN = {0167-594X},
url = {http://dx.doi.org/10.1007/s11060-015-1969-9},
Abstract = {Pediatric high-grade gliomas (pHGGs) occur with strikingly
different frequencies in infratentorial and supratentorial
regions. Although histologically these malignancies appear
similar, they represent distinct diseases. Recent genomic
studies have identified histone K27M H3.3/H3.1 mutations in
the majority of brainstem pHGGs; these mutations are rarely
encountered in pHGGs that arise in the cerebral cortex.
Previous research in brainstem pHGGs suggests a restricted
permeability of the blood-brain-barrier (BBB). In this work,
we use dynamic contrast-enhanced (DCE) MRI to evaluate BBB
permeability in a genetic mouse model of pHGG as a function
of location (cortex vs. brainstem, n = 8 mice/group) and
histone mutation (mutant H3.3K27M vs. wild-type H3.3, n = 8
mice/group). The pHGG models are induced either in the
brainstem or the cerebral cortex and are driven by PDGF
signaling and p53 loss with either H3.3K27M or wild-type
H3.3. T2-weighted MRI was used to determine tumor
location/extent followed by 4D DCE-MRI for estimating the
rate constant (K (trans) ) for tracer exchange across the
barrier. BBB permeability was 67 % higher in cortical pHGGs
relative to brainstem pHGGs (t test, p = 0.012) but was not
significantly affected by the expression of mutant H3.3K27M
versus wild-type H3.3 (t-test, p = 0.78). Although mice
became symptomatic at approximately the same time, the mean
volume of cortical tumors was 3.6 times higher than the mean
volume of brainstem tumors. The difference between the mean
volume of gliomas with wild-type and mutant H3.3 was
insignificant. Mean K (trans) was significantly correlated
to glioma volume. These results present a possible
explanation for the poor response of brainstem pHGGs to
systemic therapy. Our findings illustrate a potential role
played by the microenvironment in shaping tumor growth and
BBB permeability.},
Doi = {10.1007/s11060-015-1969-9},
Key = {fds292753}
}
@booklet{Utz87,
Author = {Utz, JA and Herfkens, RJ and Johnson, CD and Shimakawa, A and Pelc, N and Glover, G and Johnson, GA and Spritzer, CE},
Title = {Two-second MR images: comparison with spin-echo images in 29
patients.},
Journal = {AJR Am J Roentgenol},
Volume = {148},
Number = {3},
Pages = {629-633},
Year = {1987},
Month = {March},
ISSN = {0361-803X},
url = {http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:A1987G131300036&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=47d3190e77e5a3a53558812f597b0b92},
Abstract = {MR images can be obtained with a 2-sec scan time when an
extremely short repetition rate (22 msec), limited flip
angle (30 degrees), and gradient refocused echoes are used.
Comparison of 415 such images obtained in 29 patients with
routine T1-weighted (TR 500, TE 25) and T2-weighted (TR
2000, TE 80) images showed that images free of respiratory
artifacts could be obtained in all patients. Although
abdominal organs were well seen with 2-sec scan time,
overall evaluation of these organs was better on routine
T1-weighted images. Vascular structures, however, were seen
as well or better on the 2-sec images in 60% of cases. The
images were extremely sensitive to field nonhomogeneity, and
metallic artifact was exaggerated in five patients with
surgical clips. Two-sec MR images provide a rapid method of
localizing abdominal organs for further evaluation. The
sensitivity to blood flow may assist in the assessment of
vascular patency.},
Doi = {10.2214/ajr.148.3.629},
Key = {Utz87}
}
@booklet{Wendling83,
Author = {M. G. Wendling and D. W. Ducharme and G. A. Johnson and R.
B. Mccall and D. T. Pals},
Title = {U-54,669f - a novel hypotensive agent},
Journal = {Federation Proceedings},
Volume = {42},
Number = {2},
Pages = {162 -- 166},
Year = {1983},
Key = {Wendling83}
}
@article{fds174067,
Author = {MG Wendling and DW DuCharme and GA Johnson and RB McCall and DT
Pals},
Title = {U-54,669F: a novel hypotensive agent.},
Journal = {Federation proceedings},
Volume = {42},
Number = {2},
Pages = {162-6},
Year = {1983},
Month = {February},
ISSN = {0014-9446},
Keywords = {Angiotensin II • Animals • Antihypertensive
Agents* • Blood Pressure • Cats •
Dose-Response Relationship, Drug • Electric Stimulation
• Female • Heart Rate • Macaca fascicularis
• Male • Norepinephrine • Piperazines •
Rats • Rats, Inbred Strains • blood • drug
effects • drug effects* • pharmacology •
pharmacology*},
Abstract = {U-54,669F, a new antihypertensive agent, administered orally
was associated with dose-related hypotensive responses in
conscious, spontaneously hypertensive, and normotensive rats
(0.015-0.5 mg/kg) and in supine conscious monkeys (1-10
mg/kg). No loss of hypotensive efficacy of U-54,669F was
observed after 1 wk of daily repetitive treatment. U-54,669F
did not alter electrical postganglionic sympathetic nerve
activity or postsynaptic sympathetic function. Hypotensive
responses to U-54,669F were blunted in spinal cats.
U-54,669F was associated with dose-related decreases in
norepinephrine (NE) levels in plasma and in cardiac and
splenic tissue, whereas brain NE was unaltered. U-54,669F
attenuated vascular responses associated with electrical
stimulation of sympathetic nerves. However, at hypotensive
doses, U-54,669F did not impair the ability of monkeys to
withstand orthostatic stress, or contraction of the
nictitating membrane secondary to sympathetic stimulation in
the cat. U-54,669F appears to alter peripheral sympathetic
neurogenic function, but apparently does not enter the
central nervous system and does not impair the ability to
withstand orthostatic stress at effective hypotensive
doses.},
Language = {eng},
Key = {fds174067}
}
@article{fds268839,
Author = {Howles, GP and Qi, Y and Johnson, GA},
Title = {Ultrasonic disruption of the blood-brain barrier enables in
vivo functional mapping of the mouse barrel field cortex
with manganese-enhanced MRI.},
Journal = {Neuroimage},
Volume = {50},
Number = {4},
Pages = {1464-1471},
Year = {2010},
Month = {May},
ISSN = {1095-9572},
url = {http://www.ncbi.nlm.nih.gov/pubmed/20096789},
Keywords = {Animals • Blood-Brain Barrier • Brain Mapping
• Cerebral Cortex • Conscious Sedation •
Imaging, Three-Dimensional • Magnetic Resonance Imaging
• Manganese • Mice • Mice, Inbred C57BL
• Microbubbles • Physical Stimulation • Touch
Perception • Ultrasonics • Ultrasonography,
Doppler, Transcranial • Vibrissae • metabolism
• metabolism* • methods* • physiology •
physiology*},
Abstract = {Though mice are the dominant model system for studying the
genetic and molecular underpinnings of neuroscience,
functional neuroimaging in mice remains technically
challenging. One approach, Activation-Induced
Manganese-enhanced MRI (AIM MRI), has been used successfully
to map neuronal activity in rodents. In AIM MRI,
manganese(2+) acts a calcium analog and accumulates in
depolarized neurons. Because manganese(2+) shortens T1,
regions of elevated neuronal activity enhance in MRI.
However, because manganese does not cross the blood-brain
barrier (BBB), the need to osmotically disrupt the BBB has
limited the use of AIM MRI, particularly in mice. In this
work, the BBB was opened in mice using unfocused,
transcranial ultrasound in combination with gas-filled
microbubbles. Using this noninvasive technique to open the
BBB bilaterally, manganese could be quickly administered to
the whole mouse brain. With this approach, AIM MRI was used
to map the neuronal response to unilateral mechanical
stimulation of the vibrissae in lightly sedated mice. The
resultant 3D activation map agreed well with published
representations of the vibrissae regions of the barrel field
cortex. The anterior portions of the barrel field cortex
corresponding to the more rostral vibrissae showed greater
activation, consistent with previous literature. Because the
ultrasonic opening of the BBB is simple, fast, and
noninvasive, this approach is suitable for high-throughput
and longitudinal studies in awake mice. This approach
enables a new way to map neuronal activity in mice with
manganese.},
Language = {eng},
Doi = {10.1016/j.neuroimage.2010.01.050},
Key = {fds268839}
}
@article{fds174097,
Author = {GA Johnson and D Robinson and M Hornung},
Title = {Unique bedrock and soils associated with the Teesdale
flora.},
Journal = {Nature},
Volume = {232},
Number = {5311},
Pages = {453-6},
Year = {1971},
Month = {August},
ISSN = {0028-0836},
url = {http://dx.doi.org/10.1038/232453a0},
Language = {eng},
Doi = {10.1038/232453a0},
Key = {fds174097}
}
@article{fds174219,
Author = {TT Kawabe and MF Kubicek and GA Johnson and AE Buhl},
Title = {Use of gamma-glutamyl transpeptidase activity as a marker of
hair cycle and anagen induction in mouse hair
follicles.},
Journal = {The Journal of investigative dermatology},
Volume = {103},
Number = {1},
Pages = {122-6},
Year = {1994},
Month = {July},
ISSN = {0022-202X},
Keywords = {Animals • Biological Markers • Cell Cycle •
Dose-Response Relationship, Drug • Female • Hair
• Histocytochemistry • Male • Mice •
Minoxidil • analysis • analysis* • cytology*
• drug effects • enzymology* •
gamma-Glutamyltransferase • pharmacology •
physiology},
Abstract = {gamma-glutamyl transpeptidase activity was monitored in
cycling mice by histologic localization and biochemical
assay. Our objective for this study is to establish the
relationship between gamma-glutamyl transpeptidase activity
and hair growth and to determine whether its activity can be
correlated to induced hair growth. In cycling mouse skin,
gamma-glutamyl transpeptidase activity is pronounced during
anagen and greatly diminished during telogen. In the skin,
the enzyme is present exclusively in the outer and inner
root sheaths of hair follicles. gamma-glutamyl
transpeptidase is limited to the follicle below the level of
the sebaceous gland and is completely absent in the follicle
above the sebaceous gland level. During anagen, the outer
root sheath in the hypodermis is intensely positive for
gamma-glutamyl transpeptidase activity whereas the hair
matrix cells and dermal papillar are negative. The inner
root sheath above the bulb shows distinctive membrane
staining for gamma-glutamyl transpeptidase. gamma-glutamyl
transpeptidase activity can be seen to vary only in cycling
follicles. Inducing anagen by plucking hair shafts results
in an increase in gamma-glutamyl transpeptidase activity
directly correlated to hair regrowth. In a similar manner,
mice were plucked and treated with a daily dose of 2%
minoxidil. A slight difference in cycle lengths was seen in
animals treated with minoxidil when compared to vehicle
control. Minoxidil treatment may cause an early initiation
of anagen, but both the minoxidil-treated skin and the
vehicle-treated skin entered telogen at the same time.
Together, these studies indicate that gamma-glutamyl
transpeptidase is a specific marker of anagen in growing
hair.},
Language = {eng},
Key = {fds174219}
}
@booklet{Kawabe94,
Author = {T. T. Kawabe and M. F. Kubicek and G. A. Johnson and A. E.
Buhl},
Title = {Use of gamma-glutamyl-transpeptidase activity as a marker of
hair cycle and anagen induction in mouse
hair-follicles},
Journal = {Journal Of Investigative Dermatology},
Volume = {103},
Number = {1},
Pages = {122 -- 126},
Year = {1994},
Month = {July},
Key = {Kawabe94}
}
@article{fds268749,
Author = {MacFall, JS and Johnson, GA},
Title = {Use of magnetic resonance imaging in the study of plants and
soils},
Volume = {36},
Number = {36},
Pages = {99-113},
Publisher = {SOIL SCIENCE SOC AMER},
Editor = {Anderson, SH and Hopmans, JW},
Year = {2015},
Month = {October},
ISBN = {9780891188087},
ISSN = {0081-1904},
url = {http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:A1994BB18K00009&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=47d3190e77e5a3a53558812f597b0b92},
Abstract = {The nondestructive study of plants and soils in situ has
historically been difficult due to the inherently opaque
nature of the system. Repeated study of root growth and
physiological processes in bulk soil has been impossible
without techniques for remote sensing, such as x-ray,
computed tomography, rhizotrons, or magnetic resonance
imaging (MRI). Magnetic resonance imaging techniques are now
providing tools for the direct, nondestructive study of
plants and soils. New smaller bore, high field MRI scanners
can provide resolutions (10-100 μm) far superior to
resolutions provided by clinical scanners (>1 mm), and can
truly be considered magnetic resonance microscopy (MRM).
Images can be repeatedly acquired over time, providing both
spatial and temporal information. The technique has provided
information on water extraction by roots, root growth and
function, flow of water through porous media, water
distribution, and binding patterns in sand-sandstone and in
planta, and plant physiological processes. The chemical
environment can also be probed by examinations of relaxation
times and diffusion coefficients measured by MRI. As the
technique is further developed, many new and unique
applications will be found.},
Doi = {10.2136/sssaspecpub36.c9},
Key = {fds268749}
}
@booklet{Brown90,
Author = {Brown, JM and Kramer, PJ and Cofer, GP and Johnson,
GA},
Title = {Use of nuclear magnetic resonance microscopy for noninvasive
observations of root-soil water relations},
Journal = {Theoretical and Applied Climatology},
Volume = {42},
Number = {4},
Pages = {229-236},
Publisher = {Springer Nature},
Year = {1990},
Month = {December},
ISSN = {0177-798X},
url = {http://dx.doi.org/10.1007/BF00865983},
Abstract = {As part of our strategy to study root-soil water
relationships, it was necessary to develop a nondestructive
technique to detect small changes in water distribution in
and near the root. Nuclear magnetic resonance imaging (MRI),
clinically used to nondestructively and noninvasively
acquire anatomical information, can also be used to observe
water distribution in roots, soils and other plant tissues.
In MRI, a sample is placed in a strong magnetic field and a
sequence of radio frequency (rf) pulses and magnetic field
gradients is used to measure the concentration and
relaxation properties of protons, chiefly those associated
with water. This information is then reconstructed into a
digital image representing the spatial distribution of water
in plant tissues and soil. Today, intact roots less than 1
mm in diameter growing in soil or synthetic media can be
clearly imaged in less than 4 minutes at resolutions
typically less than 30μm. This permits rapid production of
images that simultaneously distinguish temporal changes in
water distribution in root tissue, the rhizosphere and the
adjacent soil at microscopic levels. Applications of this
technique for investigating plant-soil water relationships
will be discussed. © 1990 Springer-Verlag.},
Doi = {10.1007/BF00865983},
Key = {Brown90}
}
@booklet{Tengowski01,
Author = {Tengowski, MW and Suddarth, SA and Cofer, GP and Wheeler, CT and Botts,
S and Fasulo, LM and Jeffries-Griffor, JL and Amacher, DE and Lawton,
MP and Hedlund, LW and Zhang, XW and Burkhardt, JE and Johnson,
GA},
Title = {Using technology to develop a hepatic lipidosis blomarker in
the rat},
Journal = {MOLECULAR BIOLOGY OF THE CELL},
Volume = {12},
Pages = {261A-261A},
Publisher = {AMER SOC CELL BIOLOGY},
Year = {2001},
Month = {November},
ISSN = {1059-1524},
url = {http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000172372501425&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=47d3190e77e5a3a53558812f597b0b92},
Key = {Tengowski01}
}
@article{fds204261,
Author = {FW Bazer and G Wu and GA Johnson and J Kim and G Song},
Title = {Uterine histotroph and conceptus development: select
nutrients and secreted phosphoprotein 1 affect mechanistic
target of rapamycin cell signaling in ewes.},
Journal = {Biology of reproduction},
Volume = {85},
Number = {6},
Pages = {1094-107},
Year = {2011},
Month = {December},
ISSN = {1529-7268},
url = {http://dx.doi.org/10.1095/biolreprod.111.094722},
Abstract = {Interferon tau (IFNT), the pregnancy recognition signal in
ruminants, abrogates the uterine luteolytic mechanism to
ensure maintenance of function for the corpora lutea to
produce progesterone (P4). IFNT also suppresses expression
of classical IFN-stimulated genes by uterine lumenal
epithelium (LE) and superficial glandular (sGE) epithelium
but, acting in concert with progesterone, affects expression
of a multitude of genes critical to growth and development
of the conceptus. The LE and sGE secrete proteins and
transport nutrients into the uterine lumen necessary for
conceptus development, pregnancy recognition signaling, and
implantation. Secretions include arginine and secreted
phosphoprotein 1 (SPP1). Arginine can be metabolized to
nitric oxide and to polyamines or act directly to activate
the mechanistic target of rapamycin cell signaling pathway
to stimulate proliferation, migration, and mRNA translation
in trophectoderm cells. SPP1 binds alphavbeta3 and
alpha5beta1 integrins to induce focal adhesion assembly,
adhesion, and migration of conceptus trophectoderm cells
during implantation. Thus, arginine and SPP1 mediate growth,
migration, cytoskeletal remodeling, and adhesion of
trophectoderm essential for pregnancy recognition signaling
and implantation. This minireview focuses on components of
histotroph that affect conceptus development in the
ewe.},
Language = {eng},
Doi = {10.1095/biolreprod.111.094722},
Key = {fds204261}
}
@article{fds174090,
Author = {MM Joyce, JR Burghardt and RC Burghardt and RN Hooper and FW Bazer and GA Johnson},
Title = {Uterine MHC class I molecules and beta 2-microglobulin are
regulated by progesterone and conceptus interferons during
pig pregnancy.},
Journal = {Journal of immunology (Baltimore, Md. : 1950)},
Volume = {181},
Number = {4},
Pages = {2494-505},
Year = {2008},
Month = {August},
ISSN = {1550-6606},
Keywords = {Animals • Embryo Implantation • Endometrium •
Female • Histocompatibility Antigens Class I •
Interferon Type I • Interferon-gamma • Interferons
• Maternal-Fetal Exchange • Pregnancy •
Pregnancy Proteins • Progesterone • RNA, Messenger
• Random Allocation • Swine • Uterus •
beta 2-Microglobulin • biosynthesis •
biosynthesis* • embryology • genetics •
immunology • immunology* • metabolism •
metabolism* • physiology • physiology* •
secretion • secretion*},
Abstract = {MHC class I molecules and beta(2)-microglobulin (beta(2)m)
are membrane glycoproteins that present peptide Ags to TCRs,
and bind to inhibitory and activating receptors on NK cells
and other leukocytes. They are involved in the
discrimination of self from non-self. Modification of these
molecules in the placenta benefits pregnancy, but little is
known about their genes in the uterus. We examined the
classical class I swine leukocyte Ags (SLA) genes SLA-1,
SLA-2, and SLA-3, the nonclassical SLA-6, SLA-7, and SLA-8
genes, and the beta(2)m gene in pig uterus during pregnancy.
Uterine SLA and beta(2)m increased in luminal epithelium
between days 5 and 9, then decreased between days 15 and 20.
By day 15 of pregnancy, SLA and beta(2)m increased in stroma
and remained detectable through day 40. To determine effects
of estrogens, which are secreted by conceptuses to prevent
corpus luteum regression, nonpregnant pigs were treated with
estradiol benzoate, which did not affect the SLA or beta(2)m
genes. In contrast, progesterone, which is secreted by
corpora lutea, increased SLA and beta(2)m in luminal
epithelium, whereas a progesterone receptor antagonist
(ZK137,316) ablated this up-regulation. To determine effects
of conceptus secretory proteins (CSP) containing IFN-delta
and IFN-gamma, nonpregnant pigs were implanted with
mini-osmotic pumps that delivered CSP to uterine horns. CSP
increased SLA and beta(2)m in stroma. Cell-type specific
regulation of SLA and beta(2)m genes by progesterone and
IFNs suggests that placental secretions control expression
of immune regulatory molecules on uterine cells to provide
an immunologically favorable environment for survival of the
fetal-placental semiallograft.},
Language = {eng},
Key = {fds174090}
}
@article{fds204263,
Author = {FW Bazer and TE Spencer and GA Johnson and RC Burghardt},
Title = {Uterine receptivity to implantation of blastocysts in
mammals.},
Journal = {Frontiers in bioscience (Scholar edition)},
Volume = {3},
Pages = {745-67},
Year = {2011},
ISSN = {1945-0524},
Keywords = {Animals • Embryo Implantation • Endometrium •
Female • Humans • Pregnancy • Receptors,
Steroid • Species Specificity • Trophoblasts
• Uterus • metabolism • metabolism* •
physiology*},
Abstract = {Reproduction in mammals is a highly complex biological
process. The critical importance of reproduction to
propagation of species required the natural evolution of
various strategies that vary considerably across species.
Regardless of species, a dialogue between the developing
conceptus (embryo-fetus and associated placental membranes)
and maternal uterus must be established during the
peri-implantation period. The uterus must provide a
microenvironment that supports growth and development of the
conceptus and is receptive to implantation. During the same
period, the conceptus must provide its pregnancy recognition
signaling to sustain the functional life of corpora lutea
for production of progesterone which is essential for
implantation and placentation; critical events for
successful pregnancy. However, it is within the
peri-implantation period that most embryonic deaths occur
due to deficiencies attributed to uterine functions or to
the failure of the conceptus to develop appropriately,
signal pregnancy recognition and/or undergo implantation and
placentation. The challenge is to understand the complexity
of key mechanisms that are characteristic of successful
reproduction in humans and animals and to use that knowledge
to enhance fertility and reproductive health or to establish
acceptable methods for control of fertility.},
Language = {eng},
Key = {fds204263}
}
@article{fds157077,
Author = {OB Boyko, SR Alston and GN Fuller and CM Hulette and GA Johnson and PC
Burger},
Title = {Utility of postmortem magnetic resonance imaging in clinical
neuropathology.},
Journal = {Archives of pathology & laboratory medicine, UNITED
STATES},
Volume = {118},
Number = {3},
Pages = {219-25},
Year = {1994},
Month = {March},
ISSN = {0003-9985},
Keywords = {Autopsy • Brain • Central Nervous System •
Central Nervous System Neoplasms • Cerebrovascular
Disorders • Humans • Magnetic Resonance Imaging*
• Multiple Sclerosis • Nervous System Diseases
• injuries • methods* • pathology •
pathology*},
Abstract = {Over 200 brains were examined by postmortem magnetic
resonance imaging to determine the utility of this imaging
procedure as an adjunct to the standard postmortem
examination of the brain and spinal cord. One unembalmed
cadaver was also studied using a conventional 1.5-tesla (T)
field-strength unit, and three formalin-fixed sections of
the hippocampus were imaged using a high field-strength
(7.0-T) prototype imaging system. The postmortem magnetic
resonance images proved to be an invaluable aid that
complemented the standard pathologic examination of the
brain and spinal cord. The compelling advantages of this
postmortem radiographic procedure included the
three-dimensional aspects of the images; the ability to
detect mineral (ie, iron) deposits; small focal lesions such
as hemorrhages or infarcts; and the ability to evaluate the
extent of cerebral edema. For the same reasons, as well as
its archival potential for documenting the topographic
distribution of pathologic processes, this technique has
great promise for forensic cases. High field-strength
(7.0-T) imaging brought the resolution of magnetic resonance
to the microscopic level and reaffirmed the potential value
of magnetic resonance imaging for diagnostic and
investigative studies in which both the histologic and fine
radiologic features of lesions are of interest.},
Key = {fds157077}
}
@booklet{Boyko94,
Author = {Boyko, OB and Alston, SR and Fuller, GN and Hulette, CM and Johnson, GA and Burger, PC},
Title = {Utility of postmortem magnetic resonance imaging in clinical
neuropathology.},
Journal = {Arch Pathol Lab Med},
Volume = {118},
Number = {3},
Pages = {219-225},
Year = {1994},
Month = {March},
ISSN = {0003-9985},
url = {http://www.ncbi.nlm.nih.gov/pubmed/8135623},
Abstract = {Over 200 brains were examined by postmortem magnetic
resonance imaging to determine the utility of this imaging
procedure as an adjunct to the standard postmortem
examination of the brain and spinal cord. One unembalmed
cadaver was also studied using a conventional 1.5-tesla (T)
field-strength unit, and three formalin-fixed sections of
the hippocampus were imaged using a high field-strength
(7.0-T) prototype imaging system. The postmortem magnetic
resonance images proved to be an invaluable aid that
complemented the standard pathologic examination of the
brain and spinal cord. The compelling advantages of this
postmortem radiographic procedure included the
three-dimensional aspects of the images; the ability to
detect mineral (ie, iron) deposits; small focal lesions such
as hemorrhages or infarcts; and the ability to evaluate the
extent of cerebral edema. For the same reasons, as well as
its archival potential for documenting the topographic
distribution of pathologic processes, this technique has
great promise for forensic cases. High field-strength
(7.0-T) imaging brought the resolution of magnetic resonance
to the microscopic level and reaffirmed the potential value
of magnetic resonance imaging for diagnostic and
investigative studies in which both the histologic and fine
radiologic features of lesions are of interest.},
Key = {Boyko94}
}
@article{fds268791,
Author = {Bowsher, JE and Yuan, H and Hedlund, LW and Turkington, TG and Akabani,
G and Badea, A and Kurylo, WC and Ted Wheeler and C and Cofer, GP and Dewhirst, MW and Allan Johnson and G},
Title = {Utilizing MRI information to estimate F18-FDG distributions
in rat flank tumors},
Journal = {IEEE Nuclear Science Symposium Conference
Record},
Volume = {4},
Pages = {2488-2492},
Year = {2004},
Month = {December},
ISSN = {1095-7863},
Abstract = {This paper investigates the potential of magnetic resonance
imaging (MRI) to improve the estimation of within-tumor
variations in F18-FDG concentration. An image model is
described for incorporating MRI images into positron
emission tomography (PET) and single photon emission
computed tomog-raphy (SPECT) radiotracer image
reconstruction. The model promotes greater smoothing, of
estimated radiotracer concentration, among nearby voxels
that have more nearly similar MRI signals. R3230 mammary
adenocarcinomas are grown on rat flanks. Autoradiography,
histology, and T2-weighted MRI are used to demonstrate that
the above image model accurately reflects true F18-FDG
distributions in R3230 tumors. In vivo F18-FDG distributions
are then reconstructed from PET projection data, with and
without incorporating MRI. The F18-FDG images reconstructed
with MRI show greater detail, and this additional detail is
consistent with the results of the autoradiography and
histology studies. © 2004 IEEE.},
Key = {fds268791}
}
@booklet{Walsh01,
Author = {B. Walsh and G. A. Johnson},
Title = {Validation: Never an endpoint: A systems development life
cycle approach to good clinical practice},
Journal = {Drug Information Journal},
Volume = {35},
Number = {3},
Pages = {809 -- 817},
Year = {2001},
Key = {Walsh01}
}
@article{fds351448,
Author = {Wang, N and Anderson, RJ and Ashbrook, DG and Gopalakrishnan, V and Park, Y and Priebe, CE and Qi, Y and Laoprasert, R and Vogelstein, JT and Williams, RW and Johnson, GA},
Title = {Variability and heritability of mouse brain structure:
Microscopic MRI atlases and connectomes for diverse
strains.},
Journal = {Neuroimage},
Volume = {222},
Pages = {117274},
Year = {2020},
Month = {November},
url = {http://dx.doi.org/10.1016/j.neuroimage.2020.117274},
Abstract = {Genome-wide association studies have demonstrated
significant links between human brain structure and common
DNA variants. Similar studies with rodents have been
challenging because of smaller brain volumes. Using high
field MRI (9.4 T) and compressed sensing, we have achieved
microscopic resolution and sufficiently high throughput for
rodent population studies. We generated whole brain
structural MRI and diffusion connectomes for four diverse
isogenic lines of mice (C57BL/6J, DBA/2J, CAST/EiJ, and
BTBR) at spatial resolution 20,000 times higher than human
connectomes. We measured narrow sense heritability (h2) I.e.
the fraction of variance explained by strains in a simple
ANOVA model for volumes and scalar diffusion metrics, and
estimates of residual technical error for 166 regions in
each hemisphere and connectivity between the regions.
Volumes of discrete brain regions had the highest mean
heritability (0.71 ± 0.23 SD, n = 332), followed by
fractional anisotropy (0.54 ± 0.26), radial diffusivity
(0.34 ± 0.022), and axial diffusivity (0.28 ± 0.19).
Connection profiles were statistically different in 280 of
322 nodes across all four strains. Nearly 150 of the
connection profiles were statistically different between the
C57BL/6J, DBA/2J, and CAST/EiJ lines. Microscopic whole
brain MRI/DTI has allowed us to identify significant
heritable phenotypes in brain volume, scalar DTI metrics,
and quantitative connectomes.},
Doi = {10.1016/j.neuroimage.2020.117274},
Key = {fds351448}
}
@booklet{Brown84,
Author = {BROWN, MA and JOHNSON, GA},
Title = {VARIABLE FIELD STUDIES OF POTENTIAL PARAMAGNETIC NMR
CONTRAST AGENTS},
Journal = {MAGNETIC RESONANCE IN MEDICINE},
Volume = {1},
Number = {2},
Pages = {119-120},
Year = {1984},
ISSN = {0740-3194},
url = {http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:A1984TF94000037&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=47d3190e77e5a3a53558812f597b0b92},
Key = {Brown84}
}
@booklet{Karis86,
Author = {KARIS, JP and GLOVER, GH and JOHNSON, GA},
Title = {VARIANT ANGLE GRADIENT REFOCUSING IN 3-DIMENSIONAL MRI
MICROSCOPY},
Journal = {INVESTIGATIVE RADIOLOGY},
Volume = {21},
Number = {9},
Pages = {S34-S34},
Publisher = {LIPPINCOTT-RAVEN PUBL},
Year = {1986},
Month = {September},
ISSN = {0020-9996},
url = {http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:A1986E000900141&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=47d3190e77e5a3a53558812f597b0b92},
Key = {Karis86}
}
@booklet{Cline89,
Author = {Cline, HE and Lorensen, WE and Herfkens, RJ and Johnson, GA and Glover,
GH},
Title = {Vascular morphology by three-dimensional magnetic resonance
imaging.},
Journal = {Magn Reson Imaging},
Volume = {7},
Number = {1},
Pages = {45-54},
Year = {1989},
ISSN = {0730-725X},
url = {http://www.ncbi.nlm.nih.gov/pubmed/2918818},
Abstract = {A three-dimensional examination of blood vessels is provided
using MR data from seven cases. The vascular surfaces are
constructed with an algorithm that automatically follows the
selected artery or vein and generates a projected
three-dimensional gradient shaded image. Fast 3DFT pulse
sequences were optimized to enhance the time-of-flight
contrast of the intravascular region. By increasing the
surface threshold value in a three-dimensional head study,
the flesh of a patient's face was peeled away to demonstrate
the superfacial temporal artery. Gated cardiac images show
the great vessels and cardiac chambers. A three-dimensional
view of the aorta shows an irregular surface in the vicinity
of an adrenal tumor. 3D MR exams provide a non-invasive
technique for assessing vascular morphology in a clinical
setting.},
Doi = {10.1016/0730-725x(89)90323-8},
Key = {Cline89}
}
@article{fds132805,
Author = {HE Cline and WE Lorensen and RJ Herfkens and GA Johnson and GH
Glover},
Title = {Vascular morphology by three-dimensional magnetic resonance
imaging.},
Journal = {Magnetic resonance imaging, UNITED STATES},
Volume = {7},
Number = {1},
Pages = {45-54},
ISSN = {0730-725X},
Keywords = {Adult • Algorithms* • Animals • Blood Vessels
• Dogs • Head and Neck Neoplasms • Heart
• Humans • Image Processing, Computer-Assisted
• Magnetic Resonance Imaging • Male • anatomy
& histology • anatomy & histology* • diagnosis
• methods • methods*},
Abstract = {A three-dimensional examination of blood vessels is provided
using MR data from seven cases. The vascular surfaces are
constructed with an algorithm that automatically follows the
selected artery or vein and generates a projected
three-dimensional gradient shaded image. Fast 3DFT pulse
sequences were optimized to enhance the time-of-flight
contrast of the intravascular region. By increasing the
surface threshold value in a three-dimensional head study,
the flesh of a patient's face was peeled away to demonstrate
the superfacial temporal artery. Gated cardiac images show
the great vessels and cardiac chambers. A three-dimensional
view of the aorta shows an irregular surface in the vicinity
of an adrenal tumor. 3D MR exams provide a non-invasive
technique for assessing vascular morphology in a clinical
setting.},
Key = {fds132805}
}
@booklet{Aiken84,
Author = {J. W. Aiken and D. W. Harris and G. A. Johnson and J. H.
Ludens and C. J. Taylor and E. O. Weselcouch and G. J.
Wilson},
Title = {Vasodilator and natriuretic activity extracted from rat
atria},
Journal = {Federation Proceedings},
Volume = {43},
Number = {3},
Pages = {453 -- 453},
Year = {1984},
Key = {Aiken84}
}
@article{fds132903,
Author = {BT Chen and AT Yordanov and GA Johnson},
Title = {Ventilation-synchronous magnetic resonance microscopy of
pulmonary structure and ventilation in mice.},
Journal = {Magnetic resonance in medicine : official journal of the
Society of Magnetic Resonance in Medicine / Society of
Magnetic Resonance in Medicine, United States},
Volume = {53},
Number = {1},
Pages = {69-75},
Year = {2005},
Month = {January},
ISSN = {0740-3194},
Keywords = {Anesthesia • Animals • Contrast Media •
Imaging, Three-Dimensional • Lung • Magnetic
Resonance Imaging • Magnetic Resonance Spectroscopy
• Male • Mice • Mice, Inbred C57BL •
Pulmonary Ventilation • Respiration, Artificial •
Ventilation-Perfusion Ratio • anatomy & histology*
• diagnostic use* • methods* • physiology
• physiology*},
Abstract = {Increasing use of transgenic animal models for pulmonary
disease has raised the need for methods to assess pulmonary
structure and function in a physiologically stable mouse. We
report here an integrated protocol using magnetic resonance
microscopy with gadolinium (Gd)-labeled starburst dendrimer
(G6-1B4M-Gd, MW = 192 +/- 1 kDa, R(h) = 5.50 +/- 0.04 nm)
and hyperpolarized (3)helium ((3)He) gas to acquire images
that demonstrate pulmonary vasculature and ventilated
airways in live mice (n = 9). Registered three-dimensional
images of (1)H and (3)He were acquired during breath-hold at
2.0 T using radial acquisition (total acquisition time of 38
and 25 min, respectively). The macromolecular Gd-labeled
dendrimer (a half-life of approximately 80 min) increased
the signal-to-noise by 81 +/- 30% in the left ventricle, 43
+/- 22% in the lung periphery, and -4 +/- 5% in the chest
wall, thus increasing the contrast of these structures
relative to the less vascular surrounding tissues. A
constant-flow ventilator was developed for the mouse to
deliver varied gas mixtures of O(2) and N(2) (or (3)He)
during imaging. To avoid hypoxemia, instrumental dead space
was minimized and corrections were made to tidal volume lost
due to gas compression. The stability of the physiologic
support was assessed by the lack of spontaneous breathing
and maintenance of a constant heart rate. We were able to
stabilize the mouse for >8 hr using ventilation of 105
breath/min and approximately 0.2 mL/breath. The feasibility
of acquiring both pulmonary vasculature and ventilated
airways was demonstrated in the mouse lung with in-plane
spatial resolution of 70 x 70 microm(2) and slice thickness
of 800 microm.},
Key = {fds132903}
}
@booklet{Chen05,
Author = {Chen, BT and Yordanov, AT and Johnson, GA},
Title = {Ventilation-synchronous magnetic resonance microscopy of
pulmonary structure and ventilation in mice.},
Journal = {Magn Reson Med},
Volume = {53},
Number = {1},
Pages = {69-75},
Year = {2005},
Month = {January},
ISSN = {0740-3194},
url = {http://www.ncbi.nlm.nih.gov/pubmed/15690504},
Abstract = {Increasing use of transgenic animal models for pulmonary
disease has raised the need for methods to assess pulmonary
structure and function in a physiologically stable mouse. We
report here an integrated protocol using magnetic resonance
microscopy with gadolinium (Gd)-labeled starburst dendrimer
(G6-1B4M-Gd, MW = 192 +/- 1 kDa, R(h) = 5.50 +/- 0.04 nm)
and hyperpolarized (3)helium ((3)He) gas to acquire images
that demonstrate pulmonary vasculature and ventilated
airways in live mice (n = 9). Registered three-dimensional
images of (1)H and (3)He were acquired during breath-hold at
2.0 T using radial acquisition (total acquisition time of 38
and 25 min, respectively). The macromolecular Gd-labeled
dendrimer (a half-life of approximately 80 min) increased
the signal-to-noise by 81 +/- 30% in the left ventricle, 43
+/- 22% in the lung periphery, and -4 +/- 5% in the chest
wall, thus increasing the contrast of these structures
relative to the less vascular surrounding tissues. A
constant-flow ventilator was developed for the mouse to
deliver varied gas mixtures of O(2) and N(2) (or (3)He)
during imaging. To avoid hypoxemia, instrumental dead space
was minimized and corrections were made to tidal volume lost
due to gas compression. The stability of the physiologic
support was assessed by the lack of spontaneous breathing
and maintenance of a constant heart rate. We were able to
stabilize the mouse for >8 hr using ventilation of 105
breath/min and approximately 0.2 mL/breath. The feasibility
of acquiring both pulmonary vasculature and ventilated
airways was demonstrated in the mouse lung with in-plane
spatial resolution of 70 x 70 microm(2) and slice thickness
of 800 microm.},
Doi = {10.1002/mrm.20307},
Key = {Chen05}
}
@article{fds268836,
Author = {Mistry, NN and Qi, Y and Hedlund, LW and Johnson,
GA},
Title = {Ventilation/perfusion imaging in a rat model of airway
obstruction.},
Journal = {Magn Reson Med},
Volume = {63},
Number = {3},
Pages = {728-735},
Year = {2010},
Month = {March},
ISSN = {1522-2594},
url = {http://www.ncbi.nlm.nih.gov/pubmed/20146375},
Keywords = {Administration, Inhalation • Airway Obstruction •
Algorithms* • Animals • Disease Models, Animal*
• Female • Helium • Humans • Image
Enhancement • Isotopes • Magnetic Resonance
Imaging • Perfusion Imaging • Rats • Rats,
Inbred F344 • Reproducibility of Results •
Sensitivity and Specificity • administration & dosage
• diagnosis* • diagnostic use • diagnostic
use* • methods*},
Abstract = {The global increase in asthma, chronic obstructive pulmonary
disease, and other pulmonary diseases has stimulated
interest in preclinical rat models of pulmonary disease.
Imaging methods for study of these models is particularly
appealing since the results can be readily translated to the
clinical setting. Comprehensive understanding of lung
function can be achieved by performing registered pulmonary
ventilation and perfusion imaging studies in the same
animal. While ventilation imaging has been addressed for
small animals, quantitative pulmonary perfusion imaging has
not been feasible until recently, with our proposed
technique for quantitative perfusion imaging using multiple
contrast-agent injections and a view-sharing radial imaging
technique. Here, we combine the method with registered
ventilation imaging using hyperpolarized (3)He in an airway
obstruction rodent model. To our knowledge, this is the
first comprehensive quantitative assessment of lung function
in small animals at high spatial resolution. Standard
deviation of the log (V/Q) is used as a quantitative
biomarker to differentiate heterogeneity between the control
and treatment group. The estimated value of the biomarker
lies within the normal range of values reported in the
literature. The biomarker that was extracted using the
imaging technique described in this work showed
statistically significant differences between the control
rats and those with airway obstruction.},
Language = {eng},
Doi = {10.1002/mrm.22221},
Key = {fds268836}
}
@article{fds132724,
Author = {G.A. Johnson},
Title = {Viallon M, Cofer GP, Suddarth SA, Msller H, Chen XJ, Chawla
MS, Hedlund LW, Cr Millieux Y, Johson GA. Functional MR
microscopy of the lung with hyperpolarized 3He. Mag Reson
Med. In press 1998},
Year = {1998},
Key = {fds132724}
}
@booklet{Lester99,
Author = {Lester, DS and Johannessen, JN and Pine, PS and McGregor, GN and Johnson, GA},
Title = {Virtual neuropathology: A new approach to preclinical
pathology using magnetic resonance imaging
microscopy},
Journal = {Spectroscopy (Santa Monica)},
Volume = {14},
Number = {7},
Pages = {17-22},
Year = {1999},
Month = {December},
ISSN = {0887-6703},
Key = {Lester99}
}
@booklet{Lester00,
Author = {Lester, DS and Pine, PS and Delnomdedieu, M and Johannessen, JN and Johnson, GA},
Title = {Virtual neuropathology: three-dimensional visualization of
lesions due to toxic insult.},
Journal = {Toxicol Pathol},
Volume = {28},
Number = {1},
Pages = {100-104},
Year = {2000},
ISSN = {0192-6233},
url = {http://dx.doi.org/10.1177/019262330002800112},
Abstract = {A first-pass approach incorporating high-field magnetic
resonance imaging (MRI) was used for rapid detection of
neuropathologic lesions in fixed rat brains. This inherently
3-dimensional and nondestructive technique provides
high-resolution, high-contrast images of fixed neuronal
tissue in the absence of sectioning or staining. This
technique, magnetic resonance microscopy (MRM), was used to
identify diverse lesions in 2 well-established rat
neurotoxicity models. The intrinsic contrast in the images
delineated lesions that were identified using a battery of
histologic stains, some of which would not be used in
routine screening. Furthermore, the MRM images provided the
locations of lesions, which were verified upon subsequent
sectioning and staining of the same samples. The inherent
contrast generated by water properties is exploited in MRM
by choosing suitable pulse sequences, or proton stains. This
approach provides the potential for a comprehensive initial
MRM screen for neurotoxicity in preclinical models with the
capability for extrapolation to clinical analyses using
classical MRI.},
Doi = {10.1177/019262330002800112},
Key = {Lester00}
}
@article{fds132837,
Author = {DS Lester and PS Pine and M Delnomdedieu and JN Johannessen and GA
Johnson},
Title = {Virtual neuropathology: three-dimensional visualization of
lesions due to toxic insult.},
Journal = {Toxicologic pathology, UNITED STATES},
Volume = {28},
Number = {1},
Pages = {100-4},
ISSN = {0192-6233},
Keywords = {Animals • Brain • Image Processing,
Computer-Assisted • Kainic Acid • Magnetic
Resonance Imaging • Microscopy • Nervous System
Diseases • Neurotoxicity Syndromes • Neurotoxins
• Oxidopamine • Parkinson Disease, Secondary
• Rats • Sympatholytics • analogs &
derivatives • chemically induced • methods* •
pathology • pathology* • toxicity},
Abstract = {A first-pass approach incorporating high-field magnetic
resonance imaging (MRI) was used for rapid detection of
neuropathologic lesions in fixed rat brains. This inherently
3-dimensional and nondestructive technique provides
high-resolution, high-contrast images of fixed neuronal
tissue in the absence of sectioning or staining. This
technique, magnetic resonance microscopy (MRM), was used to
identify diverse lesions in 2 well-established rat
neurotoxicity models. The intrinsic contrast in the images
delineated lesions that were identified using a battery of
histologic stains, some of which would not be used in
routine screening. Furthermore, the MRM images provided the
locations of lesions, which were verified upon subsequent
sectioning and staining of the same samples. The inherent
contrast generated by water properties is exploited in MRM
by choosing suitable pulse sequences, or proton stains. This
approach provides the potential for a comprehensive initial
MRM screen for neurotoxicity in preclinical models with the
capability for extrapolation to clinical analyses using
classical MRI.},
Key = {fds132837}
}
@article{fds132887,
Author = {F O'Foghludha and GA Johnson},
Title = {Voltage waveform effects on output and penetration of W- and
Mo-anode mammographic tubes.},
Journal = {Physics in medicine and biology, ENGLAND},
Volume = {26},
Number = {2},
Pages = {291-303},
Year = {1981},
Month = {March},
ISSN = {0031-9155},
Keywords = {Electrodes • Female • Humans • Mammography
• Molybdenum • Tungsten • instrumentation
• methods*},
Abstract = {The output and half-value thickness (HVT) for arbitrary
voltage waveforms can be calculated if the variation of both
quantities with voltage is known under constant-potential
(ripple-free) conditions. An apparatus to study this
variation is described. It permits measurements of output
and HVT with a Si(Li) spectrometer under conditions
equivalent to constant-potential operation; it uses
mechanical or electronic gating to limit observations to
constant-potential operation; it uses mechanical or
electronic gating to limit observations to short periods
near the peaks of a sinusoidal waveform. For W anodes both
HVT and output are found to follow simple power laws, while
polynomial functions of voltage are needed for Mo anodes.
Calculations based on these data agree well with experiments
in which various ripples are simulated by altering the
gating interval. For Mo anodes, the output increases as the
ripple decreases, but the HVT changes very little. For W
anodes both quantities decrease when the ripple increases.
The implications in mammography are briefly
discussed.},
Key = {fds132887}
}
@booklet{Ofoghludha81,
Author = {O'Foghludha, F and Johnson, GA},
Title = {Voltage waveform effects on output and penetration of W- and
Mo-anode mammographic tubes.},
Journal = {Phys Med Biol},
Volume = {26},
Number = {2},
Pages = {291-303},
Year = {1981},
Month = {March},
ISSN = {0031-9155},
url = {http://www.ncbi.nlm.nih.gov/pubmed/7220605},
Abstract = {The output and half-value thickness (HVT) for arbitrary
voltage waveforms can be calculated if the variation of both
quantities with voltage is known under constant-potential
(ripple-free) conditions. An apparatus to study this
variation is described. It permits measurements of output
and HVT with a Si(Li) spectrometer under conditions
equivalent to constant-potential operation; it uses
mechanical or electronic gating to limit observations to
constant-potential operation; it uses mechanical or
electronic gating to limit observations to short periods
near the peaks of a sinusoidal waveform. For W anodes both
HVT and output are found to follow simple power laws, while
polynomial functions of voltage are needed for Mo anodes.
Calculations based on these data agree well with experiments
in which various ripples are simulated by altering the
gating interval. For Mo anodes, the output increases as the
ripple decreases, but the HVT changes very little. For W
anodes both quantities decrease when the ripple increases.
The implications in mammography are briefly
discussed.},
Doi = {10.1088/0031-9155/26/2/008},
Key = {Ofoghludha81}
}
@article{fds368127,
Author = {Du, EY and Ortega, BK and Ninoyu, Y and Williams, RW and Cofer, GP and Cook, JJ and Hornburg, KJ and Qi, Y and Johnson, GA and Friedman,
RA},
Title = {Volumetric analysis of the aging auditory pathway using high
resolution magnetic resonance histology.},
Journal = {Front Aging Neurosci},
Volume = {14},
Pages = {1034073},
Year = {2022},
url = {http://dx.doi.org/10.3389/fnagi.2022.1034073},
Abstract = {Numerous shown consequences of age-related hearing loss have
been unveiled; however, the relationship of the cortical and
subcortical structures of the auditory pathway with aging is
not well known. Investigations into neural structure
analysis remain sparse due to difficulties of doing so in
animal models; however, recent technological advances have
been able to achieve a resolution adequate to perform such
studies even in the small mouse. We utilize 12 members of
the BXD family of recombinant inbred mice and aged separate
cohorts. Utilizing novel magnetic resonance histology
imaging techniques, we imaged these mice and generated high
spatial resolution three dimensional images which were then
comprehensively labeled. We completed volumetric analysis of
12 separate regions of interest specific to the auditory
pathway brainstem nuclei and cortical areas with focus on
the effect of aging upon said structures. Our results showed
significant interstrain variation in the age-related effect
on structure volume supporting a genetic influence in this
interaction. Through multivariable modeling, we observed
heterogenous effects of aging between different structures.
Six of the 12 regions of interests demonstrated a
significant age-related effect. The auditory cortex and
ventral cochlear nucleus were found to decrease in volume
with age, while the medial division of the medial geniculate
nucleus, lateral lemniscus and its nucleus, and the inferior
colliculus increased in size with age. Additionally, no
sex-based differences were noted, and we observed a negative
relationship between auditory cortex volume and mouse
weight. This study is one of the first to perform
comprehensive magnetic resonance imaging and quantitative
analysis in the mouse brain auditory pathway
cytoarchitecture, offering both novel insights into the
neuroanatomical basis of age-related changes in hearing as
well as evidence toward a genetic influence in this
interaction. High resonance magnetic resonance imaging
provides a promising efficacious avenue in future mouse
model hearing loss investigations.},
Doi = {10.3389/fnagi.2022.1034073},
Key = {fds368127}
}
@article{fds268778,
Author = {Badea, CT and Hedlund, LW and Wheeler, CT and Mai, W and Johnson,
GA},
Title = {Volumetric micro-CT system for in vivo microscopy},
Journal = {2004 2nd IEEE International Symposium on Biomedical Imaging:
Macro to Nano},
Volume = {2},
Pages = {1377-1380},
Year = {2004},
Month = {December},
ISBN = {0780383885},
Abstract = {Two of the major barriers to improved image quality in
micro-CT are the reduced signal to noise imposed by the
smaller voxels and the effects of physiologic motion. The
most direct approach to increase the signal to noise ratio
(SNR) is to increase the flux. This is not possible in most
of laboratory and commercial micro-CT systems that are
currently in use. We adopted a design that allows the use of
high instantaneous X-ray flux combined with synchronization
to physiologic motion. High quality imaging of moving organs
such as the heart or the lungs is directly dependent on
appropriate gating techniques. For this purpose, we acquired
X-ray projections using a flexible controller to enable
sophisticated biological pulse sequences to minimize the
effects of motion. This paper reports on the development of
a volumetric micro-CT scanner dedicated to structural and
functional phenotyping of the live mouse. © 2004
IEEE.},
Key = {fds268778}
}
@article{fds132735,
Author = {G.A. Johnson},
Title = {W. L. Hall, H. Benveniste, L.W. Hedlund, G.A. Johnson, A new
in vivo method for quantitative analysis of stroke lesions
using MR microscopy. NeuroImage 3, 158-166
(1996).},
Year = {1996},
Key = {fds132735}
}
@article{fds268716,
Author = {Papp, EA and Leergaard, TB and Calabrese, E and Johnson, GA and Bjaalie,
JG},
Title = {Waxholm Space atlas of the Sprague Dawley rat
brain.},
Journal = {Neuroimage},
Volume = {97},
Pages = {374-386},
Year = {2014},
Month = {August},
ISSN = {1053-8119},
url = {http://dx.doi.org/10.1016/j.neuroimage.2014.04.001},
Abstract = {Three-dimensional digital brain atlases represent an
important new generation of neuroinformatics tools for
understanding complex brain anatomy, assigning location to
experimental data, and planning of experiments. We have
acquired a microscopic resolution isotropic MRI and DTI
atlasing template for the Sprague Dawley rat brain with 39
μm isotropic voxels for the MRI volume and 78 μm isotropic
voxels for the DTI. Building on this template, we have
delineated 76 major anatomical structures in the brain.
Delineation criteria are provided for each structure. We
have applied a spatial reference system based on internal
brain landmarks according to the Waxholm Space standard,
previously developed for the mouse brain, and furthermore
connected this spatial reference system to the widely used
stereotaxic coordinate system by identifying cranial sutures
and related stereotaxic landmarks in the template using
contrast given by the active staining technique applied to
the tissue. With the release of the present atlasing
template and anatomical delineations, we provide a new tool
for spatial orientation analysis of neuroanatomical
location, and planning and guidance of experimental
procedures in the rat brain. The use of Waxholm Space and
related infrastructures will connect the atlas to
interoperable resources and services for multi-level data
integration and analysis across reference
spaces.},
Doi = {10.1016/j.neuroimage.2014.04.001},
Key = {fds268716}
}
@article{fds268825,
Author = {Johnson, GA and Badea, A and Brandenburg, J and Cofer, G and Fubara, B and Liu, S and Nissanov, J},
Title = {Waxholm space: an image-based reference for coordinating
mouse brain research.},
Journal = {Neuroimage},
Volume = {53},
Number = {2},
Pages = {365-372},
Year = {2010},
Month = {November},
ISSN = {1095-9572},
url = {http://www.ncbi.nlm.nih.gov/pubmed/20600960},
Keywords = {Animals • Atlases as Topic • Brain •
Databases, Factual • Histology • Image Processing,
Computer-Assisted • Imaging, Three-Dimensional •
Magnetic Resonance Imaging • Male • Mice •
Mice, Inbred C57BL • Reference Standards •
Staining and Labeling • Thalamus • anatomy &
histology • anatomy & histology* • methods •
standards*},
Abstract = {We describe an atlas of the C57BL/6 mouse brain based on MRI
and conventional Nissl histology. Magnetic resonance
microscopy was performed on a total of 14 specimens that
were actively stained to enhance tissue contrast. Images
were acquired with three different MR protocols yielding
contrast dependent on spin lattice relaxation (T1), spin
spin relaxation (T2), and magnetic susceptibility (T2*).
Spatial resolution was 21.5 mum (isotropic). Conventional
histology (Nissl) was performed on a limited set of these
same specimens and the Nissl images were registered
(3D-to-3D) to the MR data. Probabilistic atlases for 37
structures are provided, along with average atlases. The
availability of three different MR protocols, the Nissl
data, and the labels provides a rich set of options for
registration of other atlases to the same coordinate system,
thus facilitating data-sharing. All the data is available
for download via the web.},
Language = {eng},
Doi = {10.1016/j.neuroimage.2010.06.067},
Key = {fds268825}
}
@article{fds204250,
Author = {GA Johnson},
Title = {Ways to communicate the threat of climate change to
health.},
Journal = {BMJ (Clinical research ed.)},
Volume = {343},
Pages = {d7376},
Year = {2011},
ISSN = {1468-5833},
Keywords = {Climate Change* • Communication* • Greenhouse
Effect • Humans • Public Health* • Social
Behavior},
Language = {eng},
Key = {fds204250}
}
@article{fds341987,
Author = {Allan Johnson and G and Wang, N and Anderson, RJ and Chen, M and Cofer, GP and Gee, JC and Pratson, F and Tustison, N and White,
LE},
Title = {Whole mouse brain connectomics.},
Journal = {J Comp Neurol},
Volume = {527},
Number = {13},
Pages = {2146-2157},
Year = {2019},
Month = {September},
url = {http://dx.doi.org/10.1002/cne.24560},
Abstract = {Methods have been developed to allow quantitative
connectivity of the whole fixed mouse brain by means of
magnetic resonance imaging (MRI). We have translated what we
have learned in clinical imaging to the very special domain
of the mouse brain. Diffusion tensor imaging (DTI) of
perfusion fixed specimens can now be performed with spatial
resolution of 45 μm3 , that is, voxels that are 21,000
times smaller than the human connectome protocol. Specimen
preparation has been optimized through an active staining
protocol using a Gd chelate. Compressed sensing has been
integrated into high performance reconstruction and post
processing pipelines allowing acquisition of a whole mouse
brain connectome in <12 hr. The methods have been
validated against retroviral tracer studies. False positive
tracts, which are especially problematic in clinical
studies, have been reduced substantially to ~28%. The
methods have been streamlined to provide high-fidelity,
whole mouse brain connectomes as a routine study. The data
package provides holistic insight into the mouse brain with
anatomic definition at the meso-scale, quantitative volumes
of subfields, scalar DTI metrics, and quantitative
tractography.},
Doi = {10.1002/cne.24560},
Key = {fds341987}
}
@article{fds338016,
Author = {Wang, N and Anderson, RJ and Badea, A and Cofer, G and Dibb, R and Qi, Y and Johnson, GA},
Title = {Whole mouse brain structural connectomics using magnetic
resonance histology.},
Journal = {Brain Struct Funct},
Volume = {223},
Number = {9},
Pages = {4323-4335},
Year = {2018},
Month = {December},
url = {http://dx.doi.org/10.1007/s00429-018-1750-x},
Abstract = {Diffusion tensor histology holds great promise for
quantitative characterization of structural connectivity in
mouse models of neurological and psychiatric conditions.
There has been extensive study in both the clinical and
preclinical domains on the complex tradeoffs between the
spatial resolution, the number of samples in diffusion
q-space, scan time, and the reliability of the resultant
data. We describe here a method for accelerating the
acquisition of diffusion MRI data to support quantitative
connectivity measurements in the whole mouse brain using
compressed sensing (CS). The use of CS allows substantial
increase in spatial resolution and/or reduction in scan
time. Compared to the fully sampled results at the same scan
time, the subtle anatomical details of the brain, such as
cortical layers, dentate gyrus, and cerebellum, were better
visualized using CS due to the higher spatial resolution.
Compared to the fully sampled results at the same spatial
resolution, the scalar diffusion metrics, including
fractional anisotropy (FA) and mean diffusivity (MD), showed
consistently low error across the whole brain (< 6.0%)
even with 8.0 times acceleration. The node properties of
connectivity (strength, cluster coefficient, eigenvector
centrality, and local efficiency) demonstrated correlation
of better than 95.0% between accelerated and fully sampled
connectomes. The acceleration will enable routine
application of this technology to a wide range of mouse
models of neurologic diseases.},
Doi = {10.1007/s00429-018-1750-x},
Key = {fds338016}
}
@article{fds174266,
Author = {K Hayashi and DW Erikson and SA Tilford and BM Bany and JA Maclean 2nd and EB Rucker 3rd and GA Johnson and TE Spencer},
Title = {Wnt genes in the mouse uterus: potential regulation of
implantation.},
Journal = {Biology of reproduction},
Volume = {80},
Number = {5},
Pages = {989-1000},
Year = {2009},
Month = {May},
ISSN = {0006-3363},
url = {http://dx.doi.org/10.1095/biolreprod.108.075416},
Keywords = {Animals • Base Sequence • Cloning, Molecular
• DNA Primers • DNA, Complementary • Embryo
Implantation • Estradiol • Female • Frizzled
Receptors • Gene Expression • Mice •
Ovariectomy • Ovary • Pregnancy •
Progesterone • RNA, Messenger • Signal
Transduction • Uterus • Wnt Proteins • drug
effects • genetics • genetics* • metabolism
• metabolism* • pharmacology},
Abstract = {Wnt genes are involved in critical developmental and growth
processes. The present study comprehensively analyzed
temporal and spatial alterations in Wnt and Fzd gene
expression in the mouse uterus during peri-implantation of
pregnancy. Expression of Wnt4, Wnt5a, Wnt7a, Wnt7b, Wnt11,
Wnt16, Fzd2, Fzd4, and Fzd6 was detected in the uterus
during implantation. Wnt4 mRNA was most abundant in the
decidua, whereas Wnt5a mRNA was restricted to the
mesometrial decidua during decidualization. Wnt7a, Wnt7b,
and Wnt11 mRNAs were abundantly detected in the endometrial
epithelia. The expression of Wnt7b was robust in the luminal
epithelium (LE) at the implantation site on Gestational Day
5, whereas Wnt11 mRNA disappeared in the LE adjacent to the
embryo in the antimesometrial implantation chamber but
remained abundant in the LE. Wnt16 mRNA was localized to the
stroma surrounding the LE on Day 4 and remained in the
stroma adjacent to the LE but not in areas undergoing the
decidual reaction. Fzd2 mRNA was detected in the decidua,
Fzd4 mRNA was in the vessels and stroma surrounding the
embryo, and Fzd6 mRNA was observed in the endometrial
epithelia, stroma, and some blood vessels during
implantation. Ovarian steroid hormone treatment was found to
regulate Wnt genes and Fzd receptors in ovariectomized mice.
Especially, single injections of progesterone stimulated
Wnt11 mRNA, and estrogen stimulated Wnt4 and Wnt7b. The
temporal and spatial alterations in Wnt genes likely play a
critical role during implantation and decidualization in
mice.},
Language = {eng},
Doi = {10.1095/biolreprod.108.075416},
Key = {fds174266}
}
@booklet{Silverman82a,
Author = {P. M. Silverman and M. Korobkin and W. M. Thompson and G. A.
Johnson and T. B. Cole and S. R. Fisher},
Title = {Work in progress - high-resolution, thin-section
computed-tomography of the larynx},
Journal = {Radiology},
Volume = {145},
Number = {3},
Pages = {723 -- 725},
Year = {1982},
Key = {Silverman82a}
}
@article{fds269036,
Author = {Silverman, PM and Korobkin, M and Thompson, WM and Johnson, GA and Cole,
TB and Fisher, SR},
Title = {Work in progress: high-resolution, thin-section computed
tomography of the larynx.},
Journal = {Radiology},
Volume = {145},
Number = {3},
Pages = {723-725},
Year = {1982},
Month = {December},
ISSN = {0033-8419},
url = {http://www.ncbi.nlm.nih.gov/pubmed/7146403},
Keywords = {Humans • Laryngeal Neoplasms • Time Factors •
Tomography, X-Ray Computed • methods* •
radiography*},
Doi = {10.1148/radiology.145.3.7146403},
Key = {fds269036}
}