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Pryer Lab : Publications since January 2016

List all publications in the database.    :chronological  alphabetical  combined listing:
%% Pryer, Kathleen M.   
   Author = {Rothfels, CJ and Pryer, KM and Li, FW},
   Title = {Next-generation polyploid phylogenetics: rapid resolution of
             hybrid polyploid complexes using PacBio single-molecule
   Journal = {New Phytologist},
   Volume = {213},
   Number = {1},
   Pages = {413-429},
   Year = {2017},
   Month = {January},
   url = {},
   Abstract = {Difficulties in generating nuclear data for polyploids have
             impeded phylogenetic study of these groups. We describe a
             high-throughput protocol and an associated bioinformatics
             pipeline (Pipeline for Untangling Reticulate Complexes
             (Purc)) that is able to generate these data quickly and
             conveniently, and demonstrate its efficacy on accessions
             from the fern family Cystopteridaceae. We conclude with a
             demonstration of the downstream utility of these data by
             inferring a multi-labeled species tree for a subset of our
             accessions. We amplified four c. 1-kb-long nuclear loci and
             sequenced them in a parallel-tagged amplicon sequencing
             approach using the PacBio platform. Purc infers the final
             sequences from the raw reads via an iterative approach that
             corrects PCR and sequencing errors and removes PCR-mediated
             recombinant sequences (chimeras). We generated data for all
             gene copies (homeologs, paralogs, and segregating alleles)
             present in each of three sets of 50 mostly polyploid
             accessions, for four loci, in three PacBio runs (one run per
             set). From the raw sequencing reads, Purc was able to
             accurately infer the underlying sequences. This approach
             makes it easy and economical to study the phylogenetics of
             polyploids, and, in conjunction with recent analytical
             advances, facilitates investigation of broad patterns of
             polyploid evolution.},
   Doi = {10.1111/nph.14111},
   Key = {fds322311}

   Author = {Schuettpelz, E and Chen, C-W and Kessler, M and Pinson, JB and Johnson,
             G and Davila, A and Cochran, AT and Huiet, L and Pryer,
   Title = {A revised generic classification of vittarioid ferns
             (Pteridaceae) based on molecular, micromorphological, and
             geographic data},
   Journal = {Taxon},
   Volume = {65},
   Number = {4},
   Pages = {708-722},
   Year = {2016},
   Month = {August},
   url = {},
   Doi = {10.12705/654.2},
   Key = {fds322312}

   Author = {Pryer, KM and Huiet, L and Li, F-W and Rothfels, CJ and Schuettpelz,
   Title = {Maidenhair Ferns, Adiantum, are Indeed Monophyletic
             and Sister to Shoestring Ferns, Vittarioids
   Journal = {Systematic Botany},
   Volume = {41},
   Number = {1},
   Pages = {17-23},
   Year = {2016},
   Month = {March},
   url = {},
   Doi = {10.1600/036364416X690660},
   Key = {fds322313}

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