Publications [#231114] of Rytas J. Vilgalys
search PubMed.Papers Published
- James, TY; Liou, S-R; Vilgalys, R, The genetic structure and diversity of the A and B mating-type genes from the tropical oyster mushroom, Pleurotus djamor.,
Fungal genetics and biology : FG & B, vol. 41 no. 8
(August, 2004),
pp. 813-825 [doi] .
(last updated on 2024/04/24)Abstract:
In most heterothallic mushroom species, inbreeding is avoided by an incompatibility system determined by two loci each with multiple alleles (the A and B mating-type loci). In this study we investigated the genetic structure of the mating-type loci in the tropical oyster mushroom Pleurotus djamor using both positional cloning and degenerate PCR methods. DNA sequences from genomic regions cosegregating with the mating-type loci of P. djamor revealed homeodomain transcription factors (A) and pheromone receptors (B), suggesting the genetic basis for mating-type determination in P. djamor is the same as in the model mushroom species. Three pheromone receptors were detected in a single homokaryotic isolate of P. djamor. Only one pair of homeodomain genes was detected in the A mating-type region. It is hypothesized that the A mating-type locus of P. djamor is comprised of only one homeodomain pair, which may explain the lower number of A mating-type alleles relative to other mushroom species.