Publications [#228444] of Steven B. Haase
search PubMed.Papers Published
- Leman, AR; Bristow, SL; Haase, SB, Analyzing transcription dynamics during the budding yeast cell cycle,
Methods in Molecular Biology, vol. 1170
(2014),
pp. 295-312, Humana Press Inc. [doi] .
(last updated on 2024/04/25)Abstract:
Assaying global cell cycle-regulated transcription in budding yeast involves extracting RNA from a synchronous population and proper normalization of detected transcript levels. Here, we describe synchronization of Saccharomyces cerevisiae cell populations by centrifugal elutriation, followed by the isolation of RNA for microarray analysis. Further, we outline the computational methods required to directly compare RNA abundance from individual time points within an experiment and to compare independent experiments. Together, these methods describe the complete workflow necessary to observe RNA abundance during the cell cycle. © Springer Science+Business Media New York 2014.