| Publications [#346888] of Tuan Vo-Dinh
Papers Published
- Crawford, BM; Strobbia, P; Wang, HN; Zentella, R; Boyanov, MI; Pei, ZM; Sun, TP; Kemner, KM; Vo-Dinh, T, In vivo detection of microRNA within plants using plasmonic nanosensors,
Proceedings of SPIE - The International Society for Optical Engineering, vol. 11082
(January, 2019), ISBN 9781510628571 [doi]
(last updated on 2024/04/19)
Abstract: Gene expression monitoring within whole plants is critical for many applications ranging from plant biology to biofuel development. Herein, we report a unique multimodal method for in vivo imaging and biosensing of nucleic acid biotargets, specifically microRNA, within whole plant leaves by integrating three complementary techniques: surface-enhanced Raman scattering (SERS), X-ray fluorescence (XRF), and plasmonics-enhanced two-photon luminescence (TPL). The method described utilizes plasmonic nanostar-based inverse molecular sentinel (iMS) nanoprobes, which not only provide large Raman signal enhancement upon target binding, but also allow for localization and quantification by XRF and plasmonics-enhanced TPL. This report lays the foundation for the use of plasmonic nanoprobes for in vivo functional imaging of nucleic acid biotargets in whole plants.
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