Publications [#285324] of Amanda Hargrove

Journal Articles

  1. Raskatov, JA; Nickols, NG; Hargrove, AE; Marinov, GK; Wold, B; Dervan, PB, "Gene expression changes in a tumor xenograft by a pyrrole-imidazole polyamide.", Proceedings of the National Academy of Sciences of the United States of AmericaOctober,, 2012, 109(40), 16041-16045 [doi].
    (last updated on 2024/04/18)

    Abstract:
    Gene regulation by DNA binding small molecules could have important therapeutic applications. This study reports the investigation of a DNA-binding pyrrole-imidazole polyamide targeted to bind the DNA sequence 5'-WGGWWW-3' with reference to its potency in a subcutaneous xenograft tumor model. The molecule is capable of trafficking to the tumor site following subcutaneous injection and modulates transcription of select genes in vivo. An FITC-labeled analogue of this polyamide can be detected in tumor-derived cells by confocal microscopy. RNA deep sequencing (RNA-seq) of tumor tissue allowed the identification of further affected genes, a representative panel of which was interrogated by quantitative reverse transcription-PCR and correlated with cell culture expression levels.