Publications [#233820] of Barbara R. Shaw

Journal Articles

  1. Wang, JX; Sergueev, DS; Shaw, BR, "The effect of a single boranophosphate substitution with defined configuration on the thermal stability and conformation of a DNA duplex.", Nucleosides, nucleotides & nucleic acidsJanuary,, 2005, 24(5-7), 951-955 (In Press.) [16248070], [doi].
    (last updated on 2024/04/24)

    Abstract:
    Substitution of one non-bridging oxygen in a natural phosphodiester internucleotide linkage with a borano (-BH3) group results in a chiral phosphorus center in boranophosphate. UV thermal melting profiles were recorded for DNA duplexes formed between a DNA 9-mer with either an Sp or a Rp boranophosphate linkage in the middle and the complementary DNA 9-mer, as well as for their unmodified parent duplex. The thermal stability of the DNA duplexes was in the order of normal > Sp borano > Rp borano. CD spectra of all three duplexes exhibited typical B-DNA profile, which closely resembled each other.