Publications [#234178] of Eric J. Toone

Journal Articles

  1. Wilfong, EM; Locklear, U; Toone, EJ, "A single step purification for autolytic zinc proteinases.", Bioorganic & medicinal chemistry lettersJanuary,, 2010, 20(1), 280-282 [19942433], [doi].
    (last updated on 2024/11/19)

    Abstract:
    We describe a novel single-step method for the purification of stromelysin-1 catalytic domain (SCD) via immobilized metal affinity chromatography under denaturing conditions that inhibit proteolytic activity followed by on-column refolding and spontaneous autolysis of the fusion peptide to yield pure, active stromelysin-1 catalytic domain. The methodology provides a general approach for the rapid purification of large quantities of zinc proteinases.