Publications [#232568] of Michael C. Fitzgerald

Journal Articles

  1. Powell, KD; Fitzgerald, MC, "Quantitative determination of protein-peptide binding constants using a new, MALDI- and H/D-exchange based technique", Proceedings 50th Asms Conference on Mass Spectrometry and Allied TopicsDecember,, 2002, 445-446.
    (last updated on 2026/01/17)

    Abstract:
    A new MALDI-based technique, stability of unpurified proteins by rates of H/D exchange (SUPREX), for quantitative determination of protein-peptide binding constants was discussed. For the study the five different type of peptide sequence were used, which includes peptide #1, peptide #2, peptide #3, peptide #4 and peptide #5. The results show that phosphate may have a larger stabilizing effect on ligands with high K d values (weak binders) than on ligands with low K d values (strong binders). It was suggested that the S-protein binds to a number of peptide ligands.