Publications [#232622] of Michael C. Fitzgerald

Journal Articles

  1. Yang, X; Wang, M; Fitzgerald, MC, "Analysis of protein folding and function using backbone modified proteins.", Bioorganic chemistryOctober,, 2004, 32(5), 438-449 [15381405], [doi].
    (last updated on 2026/01/23)

    Abstract:
    With the recent development of chemical and biological methods to introduce backbone modifications into the polypeptide chains of proteins, there have been a growing number of site-directed mutagenesis experiments focused on understanding the role of the polypeptide backbone in protein folding and function. The substitution of a main chain amide bond with an ester bond is now a popular mutation to investigate the role of the polypeptide backbone in ligand, binding, enzyme catalysis, and protein folding. Here we review the results of studies on some 25 ester-bond containing analogues from nine different protein systems. The structural, thermodynamic, and functional consequences of introducing backbone amide- to ester-bond mutations into these protein systems are discussed.