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Publications [#229760] of Mohamed A. Noor

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Peer-reviewed journal articles published

1. Rasmussen, DA; Noor, MAF. "What can you do with 0.1x genome coverage? A case study based on a genome survey of the scuttle fly Megaselia scalaris (Phoridae).." BMC genomics 10 (August, 2009): 382. [doi]
   (last updated on 2025/06/14)

   Abstract:
   

   Background

   The declining cost of DNA sequencing is making genome sequencing a feasible option for more organisms, including many of interest to ecologists and evolutionary biologists. While obtaining high-depth, completely assembled genome sequences for most non-model organisms remains challenging, low-coverage genome survey sequences (GSS) can provide a wealth of biologically useful information at low cost. Here, using a random pyrosequencing approach, we sequence the genome of the scuttle fly Megaselia scalaris and evaluate the utility of our low-coverage GSS approach.

   Results

   Random pyrosequencing of the M. scalaris genome provided a depth of coverage (0.05x0.1x) much lower than typical GSS studies. We demonstrate that, even with extremely low-coverage sequencing, bioinformatics approaches can yield extensive information about functional and repetitive elements. We also use our GSS data to develop genomic resources such as a nearly complete mitochondrial genome sequence and microsatellite markers for M. scalaris.

   Conclusion

   We conclude that low-coverage genome surveys are effective at generating useful information about organisms currently lacking genomic sequence data.