Publications [#280440] of David J. Brady
- McCain, ST; Gehm, ME; Wang, Y; Pitsianis, NP; Brady, DJ, Multimodal multiplex Raman spectroscopy optimized for in vivo chemometrics,
Progress in Biomedical Optics and Imaging Proceedings of Spie, vol. 6093
pp. 60930 -, SPIE, San Jose, CA, United States, ISSN 1605-7422 [12.646755], [doi]
(last updated on 2019/11/20)
We have designed and constructed a multimodal multiplex Raman spectrometer which uses multi-wavelength excitation to better detect signals in the presence of fluorescence by taking advantage of the shift-variance of the Raman signal with respect to excitation frequency. Coupled with partial-least-squares (PLS) regression, the technique applied to ethanol estimation in a tissue phantom achieves root-mean-squared-cross-validation errors (RMSCVE) of 9.2 mmol/L with a model formed with 2 principal components, compared to a single wavelength data set with equivalent energy where 7 principal components were used to achieve an RMSCVE of 39.1 mmol/L.
Raman spectroscopy;Signal processing;Electric excitation;Regression analysis;