Publications [#280516] of David J. Brady
- Balberg, M; Barbastathis, G; Fantini, S; Brady, DJ, Confocal imaging through scattering media with a volume holographic filter,
Smart Structures and Materials 2005: Active Materials: Behavior and Mechanics, vol. 3919
pp. 69-74, SPIE, San Jose, CA, USA [12.384180], [doi]
(last updated on 2019/11/22)
The use of a volume holographic filter as collector element in a confocal system imaging through scattering (turbid) material is described. We show that the penetration depth of the volume holographic system is de-coupled from the scatter noise discrimination properties, and is potentially more advantageous than the traditional confocal microscope. Since the volume-holographic filter is a matched-filter, the penetration depth is dependent on the mismatch of the refractive index of the sample being imaged relative to the recording conditions. We present a method to overcome this limitation based on using a pre-compensating index matching film during the recording of the volume hologram. An improvement of the penetration depth is shown experimentally.
Optical microscopy;Holography;Light scattering;Optical filters;Turbidity;