Wanda K. Neu, Professor of Biomedical Engineering and CNCS: Center for nonlinear and complex systems and Bass Fellow of Biomedical Engineering
Electroporation-Mediated Drug and Gene Delivery
Electroporation refers to the creation of small (1-40 nm) pores in the lipid bilayer of the membrane in response to large electric shocks. Electroporation occurs as an undesirable side effect in accidental contacts with high voltage wires or when defibrillation shocks are applied to the heart to prevent sudden cardiac death. However, electroporation has also important applications in biotechnology, as pores allow the introduction of drugs and DNA into cells. Still, this technique is largely empirical and the results are often variable and difficult to control. Our research in this area concentrates on the development of a model of electroporation that would allow theoretical investigation of the creation of pores during the shock, the flow of ions, drugs, and DNA through pores, and the resulting changes in intracellular concentrations.
Nonlinear Dynamics Approach to Cardiac Arrhythmias
Cardiac arrhythmias, serious and potentially fatal diseases, can only rarely be successfully controlled with medication. The only available treatment is defibrillation, the delivery of a large electric shock. Such shocks often have serious side effects. Our research investigates
the feasibility of alternative treatments for cardiac tachyarrhythmias that instead of large shocks use an appropriately timed train of small pulses. The approach is based on the feedback protocols that have been developed to control the dynamics of complex nonlinear systems and involves animal experiments, mathematical modeling, and computer simulations. These studies allow us to gain a better insight into the dynamics of cardiac rhythm and to assess the feasibility of bringing an episode of arrhythmia under control, terminate it, or even inhibit its occurrence.
| Office Location: | 1151 CIEMAS, 101 Science Drive, Durham, NC 27708 |
| Office Phone: | (919) 660-5105 |
| Email Address: |   |
| Web Page: | http://aiki.bme.duke.edu/wanda/ |
- Education:
Ph.D. Duke University 1987 M.S. Warsaw University of Technology 1978
- Specialties:
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Drug Delivery
Heart, Electrophysiology
- Research Interests:
Krassowska's research includes electroporation-mediated drug delivery and gene therapy, and control of cardiac arrhythmias using nonlinear dynamics
- Keywords:
- Action Potentials • Algorithms • Animals • Anisotropy • Arrhythmias, Cardiac • Biophysics • Cardiac Pacing, Artificial • Computer Simulation • Defibrillators, Implantable • Diastole • Dogs • Electric Countershock • Electric Stimulation • Electrodes • Electrodes, Implanted • Electrophysiologic Techniques, Cardiac • Electroporation • Endocardium • Equipment Design • Extracellular Space • Heart • Heart Atria • Heart Conduction System • Heart Rate • Heart Ventricles • Humans • Ions • Lipid Bilayers • Membrane Potentials • Models, Animal • Models, Biological • Models, Cardiovascular • Models, Neurological • Models, Theoretical • Muscle Cells • Myocardial Contraction • Needles • Nerve Fibers • Pericardium • Porous materials • Predictive Value of Tests • Rana catesbeiana • Signal Processing, Computer-Assisted • Statistics as Topic • Sulfonamides • Time Factors • Ventricular Function
- Recent Publications
(More Publications)
- Neu, WK, Analytical solution for time-dependent potentials in a fiber stimulated by an external electrode., Medical & Biological Engineering & Computing, vol. 54 no. 11 (November, 2016), pp. 1719-1725, Springer Verlag (Germany), ISSN 0140-0118 [doi] [abs]
- Neu, WK, Uncertainty in 1D and 3D models of a fiber stimulated by an external electrode, Lecture Notes in Computer Science (Including Subseries Lecture Notes in Artificial Intelligence and Lecture Notes in Bioinformatics), vol. 9656 (January, 2016), pp. 219-229, Springer International Publishing, ISBN 9783319317434 [doi] [abs]
- Neu, WK; Neu, JC, Modeling electroporation with moment dynamics equations (December, 2015)
- Neu, WK, Analytical solution for time-dependent potentials in a cylindrical fiber (December, 2015)
- Idriss, SF; Krassowska Neu, W; Varadarajan, V; Antonijevic, T; Gilani, SS; Starobin, JM, Feasibility of non-invasive determination of the stability of propagation reserve in patients, Computing in Cardiology, vol. 39 (December, 2012), pp. 353-356, ISSN 2325-8861 [abs]