Math @ Duke
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Publications [#244170] of Stephanos Venakides
Papers Published
- Peralta, XG; Toyama, Y; Hutson, MS; Montague, R; Venakides, S; Kiehart
and, DP; Edwards, GS, Resiliency, coordination, and synchronization of dorsal closure during Drosophila
morphogenesis,
Biophysical Journal, vol. 92 no. 7
(April, 2007),
pp. 2583-2596, ISSN 0006-3495 [17218455], [doi]
(last updated on 2024/04/19)
Abstract: Tissue
dynamics
during
dorsal
closure,
a
stage
of
Drosophila
development,
provide
a
model
system
for
cell
sheet
morphogenesis
and
wound
healing.
Dorsal
closure
is
characterized
by
complex
cell
sheet
movements,
driven
by
multiple
tissue
specific
forces,
which
are
coordinated
in
space,
synchronized
in
time,
and
resilient
to
UV-laser
perturbations.
The
mechanisms
responsible
for
these
attributes
are
not
fully
understood.
We
measured
spatial,
kinematic,
and
dynamic
antero-posterior
asymmetries
to
biophysically
characterize
both
resiliency
to
laser
perturbations
and
failure
of
closure
in
mutant
embryos
and
compared
them
to
natural
asymmetries
in
unperturbed,
wild-type
closure.
We
quantified
and
mathematically
modeled
two
processes
that
are
upregulated
to
provide
resiliency.contractility
of
the
amnioserosa
and
formation
of
a
seam
between
advancing
epidermal
sheets,
i.e.,
zipping.
Both
processes
are
spatially
removed
from
the
laser-targeted
site,
indicating
they
are
not
a
local
response
to
laser-induced
wounding
and
suggesting
mechanosensitive
and/or
chemosensitive
mechanisms
for
upregulation.
In
mutant
embryos,
tissue
junctions
initially
fail
at
the
anterior
end
indicating
inhomogeneous
mechanical
stresses
attributable
to
head
involution,
another
developmental
process
that
occurs
concomitant
with
the
end
stages
of
closure.
Asymmetries
in
these
mutants
are
reversed
compared
to
wild-type,
and
inhomogeneous
stresses
may
cause
asymmetries
in
wild-type
closure.
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dept@math.duke.edu
ph: 919.660.2800
fax: 919.660.2821
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Mathematics Department
Duke University, Box 90320
Durham, NC 27708-0320
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