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Publications [#208963] of Joshua Vogelstein

Papers Published

  1. Yuste, Rafael and MacLean, Jason and Vogelstein, Joshua T. and Paninski, Liam, Imaging action potentials with calcium indicators., Cold Spring Harbor protocols, vol. 2009 no. 8 (2011), pp. pdb.prot5316 [21807854]
    (last updated on 2012/09/19)

    The understanding of neuronal circuits has been, and will continue to be, greatly advanced by the simultaneous imaging of action potentials in neuronal ensembles. This protocol describes "bulk" loading of brain slices with acetoxymethyl (AM) ester calcium indicators in order to monitor action potential activity in large populations of neurons simultaneously. The imaging of calcium influx into neurons provides an indirect, but accurate, measure of action potential generation in individual neurons. Single-cell resolution, and thus the easy identification of every active cell, is the key advantage of the technique.
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