Publications [#254634] of Myron L. Wolbarsht

Journal Articles

1. Stein, PJ; Henkens, RW; Yamanashi, BS; Wolbarsht, M (1976). Studies on brunescent cataracts. II. Fluorescence studies on normal and brunescent lens proteins. Ophthalmic Research, 8(5), 388-394.
   (last updated on 2018/09/26)

   Abstract:
   The fluorescence of both water-soluble and water-insoluble protein fractions from normal and brunescent lens was measured. Among the solvents used, sodium dodecyl sulfate and guanidinium chloride do not destroy the primary structure of the lens protein. The non-amino acid fluorescence intensity increases in the insoluble fractions of both normal and cataractous lenses. Similar results were obtained using digests of the lens protein solutions. The yellow color of brunescent cataracts is associated predominantly with the fraction that is insoluble even in dodecyl sulfate and dithiothreitol. It is suggested that the fluorescence and the yellow pigmentation associated with brunescent lenses do not result from the same compound.