Dr. Wax's research interests include optical spectroscopy for early cancer detection, novel microscopy and interferometry techniques.

The study of intact, living cells with optical spectroscopy offers the opportunity to observe cellular structure, organization and dynamics in a way that is not possible with traditional methods. We have developed a set of novel spectroscopic techniques for measuring spatial, temporal and refractive structure on sub-hertz and sub-wavelength scales based on using low-coherence interferometry (LCI) to detect scattered light. We have applied these techniques in different types of cell biology experiments. In one experiment, LCI measurements of the angular pattern of backscattered light are used to determine non-invasively the structure of sub-cellular organelles in cell monolayers, and the components of epithelial tissue from freshly excised rat esophagus. This work has potential as a diagnostic method for early cancer detection. In another experiment, LCI phase measurements are used to examine volume changes of epithelial cells in a monolayer in response to environmental osmolarity changes. Although cell volume changes have been measured previously, this work demonstrates for the first time the volume of just a few cells (2 or 3) tracked continuously and in situ.

Contact Info:

Office Location:	2571 CIEMAS
Office Phone:	(919) 660-5143
Email Address:
Web Page:	http://bios.bme.duke.edu

Education:

Ph.D., Duke University, 1999

M.A., Duke University, 1996

B.S., Rensselaer Polytechnic Institute, 1993

BS, State University of New York, 1993

Curriculum Vitae

Research Interests:

Dr. Wax's research interests include optical spectroscopy for early cancer detection, novel microscopy and interferometry techniques.

Specialties:

Photonics
Medical Imaging
Cancer diagnostics and therapy
Sensing and Sensor Systems

Awards, Honors, and Distinctions

Fellow, American Institute for Medical and Biological Engineering
Fellow, International Society for Optics and Photonics
Fellow, Optical Society of America
Capers and Marion McDonald Award for Excellence in Teaching and Research, Duke University, Pratt School of Engineering
Chandran Research Award, Duke University
NIH Postdoctoral Fellowship, National Institutes of Health
NSF Early CAREER Award, National Science Foundation
ORAU Ralph E. Powe Junior Faculty Enhancement Award
W. H. Coulter Foundation Early Career Award, W. H. Coulter Foundation
Faculty Early Career Development (CAREER) Program, National Science Foundation

• PHYSICS 621.01, ADVANCED OPTICS Synopsis

  Wilkinson 126, MW 04:40 PM-05:55 PM

Representative Publications   (More Publications)

1. Pyhtila J. W., K. J. Chalut, J. D. Boyer, J. Keener, T. A. D’Amico, M. A. Gottfried, F. Gress, and A. Wax, In situ detection of nuclear atypia in Barrett’s esophagus using angle-resolved low coherence interferometry, Gastrointestinal Endoscopy, vol. 65 (2007), pp. 487-491 .
2. Chalut, KJ; Kresty, LA; Pyhtila, JW; Nines, R; Baird, M; Steele, VE; Wax, A, In situ assessment of intraepithelial neoplasia in hamster trachea epithelium using angle-resolved low-coherence interferometry., Cancer Epidemiology, Biomarkers & Prevention : a Publication of the American Association for Cancer Research, Cosponsored by the American Society of Preventive Oncology, vol. 16 no. 2 (February, 2007), pp. 223-227 [doi]  [abs].
3. Pyhtila, JW; Boyer, JD; Chalut, KJ; Wax, A, Fourier-domain angle-resolved low coherence interferometry through an endoscopic fiber bundle for light-scattering spectroscopy., Optics Letters, vol. 31 no. 6 (March, 2006), pp. 772-774 [OL.31.000772], [doi]  [abs].
4. Curry, Adam and Hwang, William L. and Wax, Adam, Epi-illumination through the microscope objective applied to darkfield imaging and microspectroscopy of nanoparticle interaction with cells in culture, Optics Express, vol. 14 no. 14 (2006), pp. 6535 - 6542 [OE.14.006535]  [abs].
5. Wax, A; Pyhtila, JW; Graf, RN; Nines, R; Boone, CW; Dasari, RR; Feld, MS; Steele, VE; Stoner, GD, Prospective grading of neoplastic change in rat esophagus epithelium using angle-resolved low-coherence interferometry., Journal of Biomedical Optics, vol. 10 no. 5 (September, 2005), pp. 051604 [1.2102767], [doi]  [abs].
6. Graf, Robert N. and Wax, Adam, Nuclear morphology measurements using Fourier domain low coherence interferometry, Optics Express, vol. 13 no. 12 (2005), pp. 4693 - 4698  [abs].
7. Pyhtila, J; Graf, R; Wax, A, Determining nuclear morphology using an improved angle-resolved low coherence interferometry system., Optics Express, vol. 11 no. 25 (December, 2003), pp. 3473-3484 [doi]  [abs].
8. Wax, A; Yang, C; Izatt, JA, Fourier-domain low-coherence interferometry for light-scattering spectroscopy., Optics Letters, vol. 28 no. 14 (July, 2003), pp. 1230-1232 [doi]  [abs].
9. Wax, A; Yang, C; Müller, MG; Nines, R; Boone, CW; Steele, VE; Stoner, GD; Dasari, RR; Feld, MS, In situ detection of neoplastic transformation and chemopreventive effects in rat esophagus epithelium using angle-resolved low-coherence interferometry., Cancer Research, vol. 63 no. 13 (July, 2003), pp. 3556-3559  [abs].
10. Wax, A; Yang, C; Backman, V; Badizadegan, K; Boone, CW; Dasari, RR; Feld, MS, Cellular organization and substructure measured using angle-resolved low-coherence interferometry., Biophysical Journal, vol. 82 no. 4 (April, 2002), pp. 2256-2264 [doi]  [abs].
11. Yang, C; Wax, A; Dasari, RR; Feld, MS, Phase-dispersion optical tomography, Optics Letters, vol. 26 no. 10 (May, 2001), pp. 686-688, The Optical Society [doi]  [abs].