Papers Published

1. Graf, RN; Wax, A, Nuclear morphology measurements using Fourier domain low coherence interferometry., Optics Express, vol. 13 no. 12 (June, 2005), pp. 4693-4698 [doi] .
   (last updated on 2023/06/01)

   Abstract:
   We present a new common path configuration Fourier domain low coherence interferometry (fLCI) optical system and demonstrate its capabilities by presenting results which determine the size of cell nuclei in a monolayer of T84 epithelial cells. The optical system uses a white light source in a modified Michelson interferometer and a spectrograph for detection of the mixed signal and reference fields. Depth resolution is obtained from the Fourier transform of the measured spectrum which provides the axial spatial cross-correlation between the signal and reference fields. The spectral dependence of scattering by the samples is determined by windowing the spectrum to measure the scattering amplitude as a function of wavenumber. We present evidence that fLCI accurately measures the longitudinal profile of cell nuclei rather than the transverse profile.

   Keywords:
   bio-optics;biomedical measurement;cellular biophysics;Fourier transform spectra;light scattering;Michelson interferometers;molecular biophysics;optical correlation;optical signal detection;signal resolution;size measurement;spectroscopic light sources;