Papers Published

1. Graf, Robert N. and Wax, Adam, Nuclear morphology measurements using Fourier domain low coherence interferometry, Optics Express, vol. 13 no. 12 (2005), pp. 4693 - 4698 .
   (last updated on 2007/04/14)

   Abstract:
   We present a new common path configuration Fourier domain low coherence interferometry (fLCI) optical system and demonstrate its capabilities by presenting results which determine the size of cell nuclei in a monolayer of T84 epithelial cells. The optical system uses a white light source in a modified Michelson interferometer and a spectrograph for detection of the mixed signal and reference fields. Depth resolution is obtained from the Fourier transform of the measured spectrum which provides the axial spatial cross-correlation between the signal and reference fields. The spectral dependence of scattering by the samples is determined by windowing the spectrum to measure the scattering amplitude as a function of wavenumber. We present evidence that fLCI accurately measures the longitudinal profile of cell nuclei rather than the transverse profile. © 2005 Optical Society of America.

   Keywords:
   Coherent light;Interferometry;Morphology;Monolayers;Fourier transforms;Spectrographs;Optical correlation;Light scattering;