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Papers Published

1. Tjong, V; Yu, H; Hucknall, A; Chilkoti, A, Amplified fluorescence detection of DNA and RNA hybridization by surface initiated enzymatic polymerization (SIEP), Technical Proceedings of the 2013 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2013, vol. 3 (August, 2013), pp. 32-36 .
   (last updated on 2024/07/16)

   Abstract:
   Detection of nucleic acid on-chip has been of great interest due to its potential as research tools and for diagnostic applications. We present a new on-chip, isothermal signal amplification technique for the direct detection of unmodified DNA and RNA on microarrays using terminal deoxy nucleotidyl transferase (TdT), a template-independent DNA polymerase that catalyzes the sequential addition of deoxynucleotides (dNTPs) at the 3'-OH group of a DNA primer and yeast poly(A) polymerase (PaP), an RNA polymerase that catalyzes polyadenylation at the 3'-OH an RNA primer. We utilized their ability to catalyze the formation of long polynucleotide and TdT's ability to incorporate unnatural nucleotide substrates such as fluorescent nucleotides (F-dNTP) into a long polymer chain of single stranded DNA (ssDNA) from the 3'-OH of a target DNA or RNA that is tethered on the surface through hybridization. We obtained dose reponse curves of hybridized DNA and RNA with LOD in -1-10 pM.