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Papers Published

1. Shimoboji, T; Stayton, PS; Long, C; Chilkoti, A; Chen, G; Harris, JM; Hoffman, AS, Site-specific conjugation of a temperature-sensitive polymer to a genetically-engineered protein, Transactions of the Annual Meeting of the Society for Biomaterials in conjunction with the International Biomaterials Symposium, vol. 1 (December, 1996), pp. 897 .
   (last updated on 2024/07/16)

   Abstract:
   A novel method for sensitive environmental control of the molecular recognition process between a protein receptor and its ligand is presented. The method is based on the conjugation of a smart, temperature-sensitive polymer, poly(N-isopropylacrylamide), to a specific genetically-engineered site, thiol. It was shown that the site-specific conjugation of a temperature-sensitive polymer to a genetically engineered protein provides a very sensitive temperature control of the ligand-protein recognition process. Such molecular switches could have widespread applications in medicine and biotechnology.

   Keywords:
   Genetic engineering;Phase transitions;Thermal effects;Biotechnology;