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Papers Published

1. Zhang, Z; Ma, H; Hausner, DB; Chilkoti, A; Beebe Jr, TP, Pretreatment of amphiphilic comb polymer surfaces dramatically affects protein adsorption, Biomacromolecules, vol. 6 no. 6 (2005), pp. 3388-3396 [16283770], [doi] .
   (last updated on 2024/04/23)

   Abstract:
   New applications in regenerative biotechnology require the ability to understand and control protein- surface interactions on micrometer and submicrometer length scales. Evidence presented here shows that micropatterned amphiphilic comb polymer films exhibit a pretreatment-dependent behavior with respect to protein adsorption for the proteins fibronectin, laminin, and for serum. A micropatterned surface, consisting of protein-reactive regions, separated by comb polymer, was created and tested for protein adsorption using the surface-sensitive imaging tool TOF-SIMS. Immersion of micropatterned surfaces in solutions of fibronectin or laminin resulted in uniform protein coverage on both the comb polymer and protein-reactive regions. However, preimmersion of similarly patterned surfaces in water for 2 h prior to protein incubation was found to dramatically improve the protein-resistant properties of the comb polymer regions. These results are consistent with poly(ethylene glycol) (PEG) side chain reorientation and/or hydration and poly-(methyl methacrylate) (PMMA) backbone segregation away from the interface region. © 2005 American Chemical Society.

   Keywords:
   Surface treatment;Proteins;Adsorption;Biotechnology;Molecular dynamics;Plastic films;Materials testing;Secondary ion mass spectrometry;Polyethylene glycols;Polymethyl methacrylates;Molecular orientation;Interfaces (materials);