Papers Published

1. Eucker, S. A. and Hoffman, B. D. and Natesh, R. and Ralston, J. and Armstead, W. M. and Margulies, S. S., Development of a fluorescent microsphere technique for rapid histological determination of cerebral blood flow, Brain Research, vol. 1326 (2010), pp. 128-134 .
   (last updated on 2011/11/11)

   Abstract:
   The purpose of this study was to develop a more efficient fluorescent microsphere method to facilitate the rapid use of the histological technique and to enable its use in large tissue regions. Using fluorescent plate/slide imaging technology and automated detection and analysis software, we were able to rapidly image, detect, and count 3 separate microsphere colors in 200 gm thick tissue sections from piglet brain. In resting newborn piglets (n=6) on isoflurane anesthesia, we measured a median total cerebral blood flow (CBF) of 105 ml/min/100 g (range 27-206 ml/min/100 g). Compared with other FM analysis methods, our method reduces the time required to determine blood flow, improves accuracy in lipid-rich tissues and large tissue regions and, unlike the radiolabeled microsphere method, can be combined with histological analysis. (C) 2010 Elsevier B.V. All rights reserved.

   Keywords:
   cerebral blood flow fluorescent microsphere pig validation pigs rat