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Papers Published

1. KL Staggs, KJ Austin, GA Johnson, MG Teixeira, CT Talbott, VA Dooley, TR Hansen, Complex induction of bovine uterine proteins by interferon-tau., Biology of reproduction, vol. 59 no. 2 (August, 1998), pp. 293-7 .
   (last updated on 2010/04/30)

   Abstract:
   Interferon-tau (IFN-tau) is released by the conceptus and induces two uterine proteins during early pregnancy: ubiquitin cross-reactive protein (UCRP) and granulocyte chemotactic protein-2 (GCP-2). The present experiments were designed to determine whether detection (Western blot) of cytosolic UCRP and release of GCP-2 could be used to examine IFN-tau signal transduction in cultured endometrial explants and primary epithelial cells. Recombinant (r) type 1 IFNs (rboIFN-tau and rboIFN-alpha; 5, 25, 100 nM) induced UCRP, but only rboIFN-tau induced GCP-2 in explant culture. Recombinant boIFN-tau and conceptus secretory proteins containing native IFN-tau induced UCRP and GCP-2 in cultured primary epithelial cells. All concentrations of rboIFN-alpha (25, 50, 100 nM) induced UCRP, but only the highest concentration induced GCP-2 in cultured primary epithelial cells. Interestingly, phorbol ester (100, 500, 1000 ng/ml) induced GCP-2, but it had no effect on UCRP. Because type 1 IFNs induce UCRP, IFN-tau probably interacts with the janus kinase (Jak)-associated IFN-alpha receptor to phosphorylate signal transducers and activators of transcription (STAT) and/or interferon regulatory factor-1 (IRF-1). However, IFN-tau-specific induction of GCP-2 may involve a variant type 1 receptor subunit or activators of transcription that are associated with protein kinase C and the Jak/STAT/IRF-1 pathway.

   Keywords:
   Animals • Blotting, Western • Cattle • Cells, Cultured • Chemokine CXCL6 • Chemokines, CXC • Chromatography, Affinity • Endometrium • Epithelial Cells • Estrus • Female • Interferon Type I • Interferon Type I, Recombinant • Pregnancy • Pregnancy Proteins • Protein Biosynthesis* • Stimulation, Chemical • Ubiquitins • Uterus • biosynthesis • drug effects • metabolism • metabolism* • pharmacology • pharmacology* • physiology