search www.ncbi.nlm.nih.gov.

Papers Published

1. GA Johnson, C Wilken, EA Van Kirk, EL Belden, WJ Murdoch, Toward regulation of gonadal function by a synthetic hybrid molecule composed of gonadotropin and Fc fragment of immunoglobulin G., American journal of reproductive immunology (New York, N.Y. : 1989), vol. 23 no. 1 (May, 1990), pp. 22-5 .
   (last updated on 2010/04/30)

   Abstract:
   A conjugate of human chorionic gonadotropin (HCG) and Fc fragment of immunoglobulin G was prepared by covalent cross-linking using the heterobifunctional reagent, N-succinimidyl 3-(2-pyridyldithio) propionate. Mouse Leydig tumor cells expressing receptors for luteinizing hormone were specifically lysed in vitro as a consequence of complement fixation via the Fc component of the hybrid molecule. Furthermore, administration of HCG-Fc to rams caused an acute depression in circulatory testosterone. This novel concept of targeted inhibition of gonadal function could prove to have future applications in control of reproductive processes.

   Keywords:
   Animals • Cell Survival • Chorionic Gonadotropin • Cross-Linking Reagents • Immunoglobulin Fc Fragments • Immunoglobulin G • Leydig Cells • Luteinizing Hormone • Male • Reproduction • Sheep • Testis • Testosterone • Tumor Cells, Cultured • blood • drug effects • drug effects* • immunology • metabolism • pharmacology*