Papers Published

1. RJ Schutte, L Xie, B Klitzman, WM Reichert, In vivo cytokine-associated responses to biomaterials., Biomaterials, vol. 30 no. 2 (January, 2009), pp. 160-8 [doi] .
   (last updated on 2010/09/23)

   Abstract:
   Cytokines, chemokines, and growth factors were analyzed periodically over eight weeks from the wound exudate fluid surrounding biomaterials implanted subcutaneously within stainless steel mesh cages. TNF-alpha, MCP-1, MIP-1alpha, IL-2, IL-6, IL-1beta, VEGF, IL-4, and IL-10 were measured from exudate samples collected from cages containing specimens of polyethylene (PE), polyurethane (PU), or organotin polyvinyl chloride (ot-PVC). Empty cages served as negative controls, and lipopolysaccharide (LPS) served as a positive control. Cytokine, chemokine, and growth factor concentrations decreased from the time of implantation to eight weeks post-implantation, and there was an overall increase in cytokine, chemokine, and growth factor production for material-containing cages compared to empty cages. However, cytokine production was only modestly affected by the different surface chemistries of the three implanted polymeric materials.

   Keywords:
   Animals • Biocompatible Materials • Chemokine CCL3 • Chemokines • Cytokines • Exudates and Transudates • Inflammation • Interleukin-1 • Interleukin-10 • Interleukin-2 • Interleukin-6 • Interleukin-8 • Lipopolysaccharides • Macrophage Inflammatory Proteins • Male • Materials Testing • Organotin Compounds • Polyethylene • Polyurethanes • Polyvinyl Chloride • Prostheses and Implants • Rats • Rats, Sprague-Dawley • Tumor Necrosis Factor-alpha • chemically induced • drug effects* • metabolism • metabolism* • pharmacology • pharmacology*