Journal Articles

1. Warnecke, TE; Lynch, MD; Lipscomb, ML; Gill, RT, Identification of a 21 amino acid peptide conferring 3-hydroxypropionic acid stress-tolerance to Escherichia coli., Biotechnology and Bioengineering, vol. 109 no. 5 (May, 2012), pp. 1347-1352 [doi] .
   (last updated on 2022/11/05)

   Abstract:
   We report the identification of a novel small open reading frame in Escherichia coli. The sORF (called iroK) encodes a 21 amino cid peptide, which when translated confers a 133% (ca. 20 g/L) increase in resistance to 3-hydroxypropionic acid. We show that iroK conferred tolerance is additive to previously identified tolerance mechanisms involving relief of inhibited metabolism, yet does not involve altered 3-HP transport. This result demonstrates the continued surprises that microbial genomes hold and emphasize the importance of comprehensive discovery methods in future strain and metabolic engineering efforts.