Publications [#140787] of John D Hamilton

Papers Published

1. SC Henry, JD Hamilton, Detection of murine cytomegalovirus immediate early 1 transcripts in the spleens of latently infected mice., The Journal of infectious diseases, UNITED STATES, vol. 167 no. 4 (April, 1993), pp. 950-4, ISSN 0022-1899
   (last updated on 2007/12/26)

   Abstract:
   The spleens of mice latently infected with murine cytomegalovirus were examined by reverse transcription followed by the polymerase chain reaction to assay for transcriptional activity of the viral immediate early 1 gene. Transcripts were detected in 6 of 8 animals at a level between 50 and 500 copies in 200 ng of total cellular RNA. This level appears to be roughly 10-fold higher than that of viral genome even though viral DNA is more readily detectable. These data suggest that a significant fraction of latent murine cytomegalovirus is transcriptionally active in the spleen, which may be indicative of an abortive infection or possibly low-level persistence or a transient reactivation.

   Keywords:
   Amino Acid Sequence • Animals • Cytomegalovirus • Female • Mice • Mice, Inbred BALB C • Molecular Sequence Data • Spleen • Transcription, Genetic • genetics* • microbiology*