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| Publications [#140862] of John D Hamilton
Papers Published
- IN Gopal, A Quinn, SC Henry, JD Hamilton, HF Staats, R Frothingham, Nasal peptide vaccination elicits CD8 responses and reduces viral burden after challenge with virulent murine cytomegalovirus.,
Microbiology and immunology, Japan, vol. 49 no. 2
(2005),
pp. 113-9, ISSN 0385-5600
(last updated on 2007/12/26)
Abstract:
Infection of BALB/c mice with murine cytomegalovirus (MCMV) leads to CD8 cell responses to an immunodominant epitope YPHFMPTNL. We presented this epitope as a nasal peptide vaccine in combination with cholera toxin adjuvant, and evaluated immune responses and protection from MCMV challenge. Vaccination of naive mice generated elevated numbers of peptide-specific interferon-gamma-secreting splenocytes (median 80/million, range 60 to 490), compared to control mice (median 2/million, range -4.5 to 8; P=0.008, Mann-Whitney test). Twelve days after challenge with virulent MCMV, vaccinated mice had a 1.1 log(10) reduction in salivary gland viral titer compared to unvaccinated controls (5.36+/-0.24 vs. 6.42+/-0.12, mean +/-SD log(10) plaque-forming-units; P <0.001, t -test). Mice with chronic MCMV infection had consistent responses to the peptide (183+/-24/million interferon-gamma-secreting splenocytes). Nasal peptide vaccination during chronic infection boosted peptide-specific responses in two of four mice to >900/million interferon-gamma-secreting splenocytes. Nasal peptide vaccination was immunogenic in naïve and MCMV-infected mice, and reduced viral burden in naive mice after virulent MCMV challenge. The nasal route may be useful for peptide presentation by novel human vaccines.
Keywords:
Adjuvants, Immunologic • Administration, Intranasal • Animals • CD8-Positive T-Lymphocytes • Cholera Toxin • Herpesviridae Infections • Immunodominant Epitopes • Interferon Type II • Lymphocyte Count • Mice • Mice, Inbred BALB C • Muromegalovirus • Salivary Glands • Spleen • Vaccination* • Vaccines, Synthetic • Viral Load • Viral Vaccines • administration & dosage • administration & dosage* • biosynthesis • immunology • immunology* • prevention & control* • virology
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