Publications [#278736] of Dennis A. Clements

Papers Published

1. Clements, DA; Wang, JK; Dionne, CA; Goldfarb, M, Activation of fibroblast growth factor (FGF) receptors by recombinant human FGF-5., Oncogene, vol. 8 no. 5 (May, 1993), pp. 1311-1316, ISSN 0950-9232 [8386828]
   (last updated on 2023/06/01)

   Abstract:
   We have purified biologically active recombinant human fibroblast growth factor 5 (FGF-5) from Escherichia coli. In the presence of heparin, recombinant FGF-5 is as active as native growth factor, demonstrating that glycosylation does not significantly potentiate FGF-5 activity. FGF-5 can bind and induce autophosphorylation of human FGF receptors (FGFR) 1 and 2. Competition binding studies show that the KD for FGF-5-FGFR-1 and FGF-5-FGFR-2 interactions are both between 0.5 and 1.5 x 10(-9) M.

   Keywords:
   3T3 Cells • Amino Acid Sequence • Animals • Base Sequence • Fibroblast Growth Factor 5 • Fibroblast Growth Factors • Humans • Mice • Molecular Sequence Data • Phosphorylation • Receptor Protein-Tyrosine Kinases* • Receptor, Fibroblast Growth Factor, Type 1 • Receptors, Cell Surface • Receptors, Fibroblast Growth Factor • Recombinant Proteins • drug effects • drug effects* • isolation & purification • metabolism • pharmacology • pharmacology*